CN105524823B - A kind of micro-fluidic chip - Google Patents
A kind of micro-fluidic chip Download PDFInfo
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- CN105524823B CN105524823B CN201410505797.0A CN201410505797A CN105524823B CN 105524823 B CN105524823 B CN 105524823B CN 201410505797 A CN201410505797 A CN 201410505797A CN 105524823 B CN105524823 B CN 105524823B
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Abstract
The invention discloses a kind of micro-fluidic chip, including：Liquid stream layer, comprising fluid course, to make the suspension of the particulate matter comprising different-diameter and carrier fluid while be circulated in the chip, the suspension and carrier fluid are in the fluid course interior edge direction layer distributed vertical with fluid flow direction；Layers of elastomeric film, include the elastic membrane being covered on fluid course；Pneumatic control layer, include an at least pneumatic control pipeline, tend to the deformation and/or displacement of fluid course to drive the regional area of elastic membrane to produce, so as to form the gap that can stop larger particles and pass through smaller particle thing and liquid in the first area of fluid course, the guiding structural for the second area that the larger particles being blocked can be imported to fluid course is formed simultaneously, and first and second region is respectively distributed in the region that suspension and carrier fluid flow through.The present invention is simple in construction, easily fabricated, is easy to use, and can realize enrichment, the separation of different-diameter particulate matter simultaneously, and is not easy to fail because blocking.
Description
Technical field
The invention belongs to microfluidic art, more particularly to a kind of micro-fluidic chip.
Background technology
As biological study is gradually deep into cell aspect, for the even single celled research of different type cell
Through as the profound important way for parsing various vital movement mechanism.For this reason, it may be necessary to sorted from a large amount of different type cells
Obtain specific cell type.Although flow cytometer is widely applied in terms of cell sorting, yet with needs
Specific fluorescent mark is carried out to analysis target, and equipment is expensive, cell requirements amount is big, it is difficult to meets various application demand.It is micro-
Fluidics is that last century the nineties grow up in analytical chemistry field, and it is led to microchannel network microstructure features
Cross micro-processing technology the function component image set such as microchannel, Micropump, micro-valve, micro- reservoir, micro- detecting element is the same into circuit,
It is integrated on chip material.Microflow control technique efficiency very high in terms of cell sorting, by making microtrabeculae, micro-pipe on chip
The structures such as road, microelectrode, a variety of different types of cells can be effectively separated in the short period, haemocyte sort,
There are multiple successful exemplary applications in terms of microorganism separation.
For example, document（Journal of Chromatography A, 1217 (2010) 1862–1866）Disclose one
The haemocyte separating chips based on microtrabeculae are planted, there is one group to form array, microtrabeculae row by multiple microtrabeculaes in the chip fluid pipe
Column direction is at an angle with liquid flow path direction（5~30°）, the gap between microtrabeculae allows the less erythroplastid stream of diameter in blood
Cross, and the erythroblast for hindering to be relatively large in diameter in blood passes through.The erythroblast being blocked i.e. edge under the promotion of liquid stream
Micro-pillar array to move to side duct wall, finally separated with less erythroplastid in liquid stream, so as to realize to different big
The separation of cellule.But the microtrabeculae to be played a role in filtering in the chip is arranged in fluid pipe, overall structure is fixed,
When occurring cell adherence on microtrabeculae or between microtrabeculae, when blocking filter gap, it is difficult to cleaning and dredging, follow-up diameter can be caused less
Cell can not be short by microtrabeculae gap, the failure of cell separation function, service life.In addition, because of micro-pillar array shape in the chip
It is fixed, Intercepting effects only are produced to the cell of special diameter, microtrabeculae gap can not be adjusted, when separation cell dia changes
When, it is necessary to separately use other microtrabeculae gaps chip, the cell dia scope of application is limited more；Postscript, using the chip only
The cell of different-diameter is separated, cell can not be enriched with simultaneously, and makes micro-pillar array to need more accurately micro- add
Work technology, and strictly control micro post diameter and gap, manufacture difficulty higher.
The content of the invention
In view of the shortcomings of the prior art, it is an object of the invention to propose a kind of particle that can realize different-grain diameter simultaneously
The separation and enrichment of thing, and micro-fluidic chip easy to process.
To realize aforementioned invention purpose, technical scheme provided by the invention includes：
A kind of micro-fluidic chip, including：
Liquid stream layer, comprising fluid course, to make the suspension of the particulate matter comprising different-diameter and carrier fluid simultaneously in described
Circulated in chip, also, the suspension and carrier fluid are in the fluid course interior edge direction layering vertical with fluid flow direction
Distribution；
Layers of elastomeric film, include the elastic membrane being covered on the fluid course；
Pneumatic control layer, comprising one or more pneumatic control pipeline, to drive the regional area of the elastic membrane to produce
Tend to the deformation and/or displacement of the fluid course, so as in the first area of the fluid course in the elastic membrane and
Being formed between fluid course bottom face can stop that particle diameter is more than the larger particles of a setting value and particle diameter is less than the setting
Gap that the smaller particle thing and liquid of value pass through, while formed and will can stopped by the gap in the fluid course
Larger particles import the guiding structural of the second area of the fluid course,
The first area and second area are respectively distributed in the region that the suspension and carrier fluid flow through.
As more preferable mode, the suspension and carrier fluid flow in the same direction in the fluid course.
Further, the pneumatic control pipeline includes thicker the pneumatic control pipeline and caliber that caliber is more than a set point value
Less than the thinner pneumatic control pipeline of the set point value, the thicker pneumatic control pipeline and thinner pneumatic control pipeline respectively with
The first area of the fluid course and second area intersect.
Further, the thicker pneumatic control pipeline gradually tilts from one first point to a second point, also, described
On suspension flow direction in fluid course, first point of upstream for being distributed in second point, and described first point is distributed in
In one region, second point is distributed in the intersection of first area and second area,
When the fluid of injection setting pressure into the thicker pneumatic control pipeline, the thicker pneumatic control pipeline drives
The elastic membrane is produced the deformation and/or displacement for tending to the fluid course bottom face, so as in the first area in
The gap and the guiding structural are formed between the elastic membrane and the fluid course simultaneously.
Further, the thicker pneumatic control pipeline is interconnected with the thinner pneumatic control pipeline.
Further, the thicker pneumatic control pipeline and the thinner pneumatic control pipeline are continuously coupled.
Further, the part intersected on the thicker pneumatic control pipeline with the fluid course has arc line shaped or straight
Linear profile.
Further, the pneumatic control pipeline include be interconnected and a continuously coupled thicker pneumatic control pipeline and
One thinner pneumatic control pipeline, the thicker pneumatic control pipeline above the first area out of described fluid course by, and
The thinner pneumatic control pipeline passes through above the second area out of described fluid course.
Further, the pneumatic control pipeline includes the one first thicker pneumatic control pipe for being interconnected and being sequentially connected
Road, a thinner pneumatic control pipeline and one second thicker pneumatic control pipeline, the first, second thicker pneumatic control pipeline point
By the way that and the thinner pneumatic control pipeline is from the fluid course above two first areas not out of described fluid course
By the way that the second area is distributed between two first areas above an interior second area.
Further, the pneumatic control pipeline includes the one first thinner pneumatic control pipe for being interconnected and being sequentially connected
Road, a thicker pneumatic control pipeline and one second thinner pneumatic control pipeline, the first, second thinner pneumatic control pipeline point
By the way that and the thicker pneumatic control pipeline is from the fluid course above two second areas not out of described fluid course
By the way that the first area is distributed between two second areas above an interior first area.
Relative to scheme of the prior art, advantages of the present invention includes：By setting pneumatic control above fluid pipe
Tubing giving sufficient strength（Drive pipeline）With elastic membrane, driving pipeline and elastic membrane in fluid pipe in the vertical direction of liquid flow path direction it is right
The particulate matter of different-diameter, such as cell granulations have different degrees of cut-off effect, so as to change major diameter particulate matter with liquid
The distribution on the vertical direction in direction is flowed, realizes the separation of major diameter particulate matter；Meanwhile can also within a certain period of time will be larger straight
The particulate matter retention in footpath is enriched in a specific region of fluid pipe, is then partially or fully discharged, is improved in fluid pipe
Particular fluid flow section in, the concentration of major diameter particulate matter, while realize separation and enrichment to major diameter particulate matter.
Compared with prior art, advantages of the present invention includes：By setting the pneumatic of different tube diameters above fluid pipe
Control pipeline（Drive pipeline）With elastic membrane, coordinated using the driving pipeline and elastic membrane of different tube diameters, can be to fluid pipe
In in the vertical direction of liquid flow path direction to the particulate matter of different-diameter, such as cell granulations produces different degrees of cut-off effect
Fruit, so as to change distribution of the different-diameter particulate matter on the direction vertical with liquid flow path direction, realize different-diameter particulate matter
Separation；Meanwhile by using the driving pipeline compared with Large Diameter Pipeline（That is, wider driving pipeline）There is certain angle to intersect with liquid flow direction
Design so that the big particulate matter of diameter can along it is wider driving pipeline profile be produced in the vertical direction with liquid flow path direction
Raw displacement, and separated with the less particulate matter of diameter, and then larger-diameter particulate matter can be retained within a certain period of time and be enriched with
In the particular fluid flow section of fluid pipe, the enrichment of major diameter particulate matter is realized, then by controlling pneumatic control pipeline
Pressurization sequential, can also be by the partially or fully release of the major diameter particulate matter in the specific region, i.e. while realize to major diameter
The separation of particulate matter and enrichment function.Moreover, if any particulate matter when being blocked on the gap of filtering, it is only necessary to remove driving tube
Air pressure in road, elastic membrane and the gap of fluid pipe wall can expand, and the particulate matter of blocking is gone out, therefore be not easy to lose because blocking
Effect；Furthermore the pressure that the diameter due to stopping particulate matter is depended in pneumatic control pipeline, therefore can be real by adjusting pressure
It is now wider to the stop of different-diameter particulate matter, applicable particulate matter scope；Postscript, due to the present invention chip in without microtrabeculae
Structure, more fine micro-processing technology requirement can be also avoided, reduce chip manufacturing difficulty.
Brief description of the drawings
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
There is the required accompanying drawing used in technology description to be briefly described, it should be apparent that, drawings in the following description are only this
Some embodiments described in invention, for those of ordinary skill in the art, on the premise of not paying creative work,
Other accompanying drawings can also be obtained according to these accompanying drawings.
Figure 1A is the top view of micro-fluidic chip in embodiment 1；
Figure 1B is the 1B along the line of micro-fluidic chip shown in Figure 1A cross-sectional view；
Fig. 2 is the top view of micro-fluidic chip in embodiment 2；
Fig. 3 is the top view of micro-fluidic chip in embodiment 3；
Fig. 4 A be embodiment 4 micro-fluidic chip in pneumatic control pipeline inject pressure medium when top view；
Fig. 4 B are the top views when removing the pressure medium in pneumatic control pipeline in micro-fluidic chip shown in Fig. 4 A；
Description of reference numerals：110 be liquid stream layer, and 120 be pneumatic control layer, and 130 be elastic membrane, 1001,2001,3001,
4001 be the cell granulations that is relatively large in diameter, and 1002,2002,3002,4002 be the less cell granulations of diameter, 1101,2101,
3101st, 4101 be fluid course, and 1201,2201,3201,4201 be the pneumatic control pipeline of narrower width, 1202,2202,
4202 be the pneumatic control pipeline of wider width, and 3202 be triangle pneumatic control pipeline, and A1, A2, A3, A4 are cell when injecting
Suspension region, B1, B2, B3, B4 are the distributed areas that cell is relatively large in diameter after separation, and C4 is the enrichment for being relatively large in diameter cell
Region, direction shown in arrow is liquid flow path direction in figure.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, detailed retouch is carried out to the technical scheme in the embodiment of the present invention
State, it is clear that described embodiment is only part of the embodiment of the present invention, rather than whole embodiments.Based on the present invention
In embodiment, the every other implementation that those of ordinary skill in the art are obtained on the premise of creative work is not made
Example, belongs to the scope of protection of the invention.
Embodiment 1 refers to Figure 1A ~ Figure 1B, and the micro-fluidic chip is made up of the structure sheaf of three stackings.Bottom is liquid stream layer
110, upper strata is pneumatic control layer 120, and flexible film 130 is separated by between two layers.There is fluid pipe 1101 in liquid stream layer 110, manage
Road is wide 100 microns, high 20 microns, and there is interface at both ends with being connected outside chip.There is width smaller in pneumatic control layer 120（10 is micro-
Rice）Pneumatic control pipeline 1201 and width it is larger（100 microns）Pneumatic control passage 1202 above fluid pipe 1101
Pass through, two pneumatic control pipeline communications, wherein pneumatic control passage 1202 intersect at an angle, its residual air with fluid pipe 1101
Dynamic wide control pipeline is 100 microns.Wherein pneumatic control pipeline 1202 passes through above liquid stream input channel 1102.Pneumatic control
Tubing giving sufficient strength is connected with the external world, and when injecting gases at high pressure into pneumatic control pipeline, the elastic membrane under pneumatic control pipeline can be to
Under bend, extrude elastic membrane below fluid pipe, when removing gases at high pressure, elastic membrane reply.This is micro-fluidic neck
Domain known technology.
Principle and action relationships explanation：
The principle that chip separation is relatively large in diameter cell is, by the cell suspension and current-carrying of the big cellule containing different-diameter
After liquid injects fluid pipe from side simultaneously, two kinds of liquid laminar flows in fluid pipe, side is cell suspension, is distributed in
A1 regions, wherein being mixed with two kinds of big cellules, opposite side is current-carrying liquid, acellular.When to pneumatic control pipeline inject high pressure
During gas, the elastic membrane of pneumatic control pipeline lower zone is bent to fluid pipe, injects gases at high pressure especially by control
Pressure, the elastic membrane of the lower section of pneumatic control pipeline 1202 is bent to fluid pipe, but keep certain with fluid pipe bottom
Gap, it is allowed to which liquid and diameter circulate compared with cellule, but can stop the major diameter cell in cell suspension, now pneumatic control pipe
Because diameter is smaller, lower section elastic membrane degree of crook is low in road 1201, can allow for liquid in the fluid pipe of lower section and various thin
Born of the same parents pass through.Due to pneumatic control pipeline 1202 and liquid stream pipeline at an angle, the cell being relatively large in diameter being trapped is along pneumatically
The boundary slip of control pipeline 1202, in the vertical direction of liquid flow path direction, it is subjected to displacement, is eventually entered into containing load to current-carrying liquid side
Flow liquid side so that the different cell of diameter is separated.
Embodiment 2 refers to Fig. 2, and the micro-fluidic chip is made up of three structure sheafs stacked on top of one another.Bottom is liquid stream layer, on
Layer is pneumatic control layer, flexible film between two layers.There is fluid pipe 2101 in liquid stream layer, pipeline is wide 100 microns, and high 20 is micro-
There is interface at rice, both ends with being connected outside chip.There are above fluid pipe 2,101 two 100 microns of width in pneumatic control layer
Pneumatic control passage 2202 is connected with each other, and there is the narrow pipeline 2201 of a width junction, on the centre of fluid pipe 2101
Orientation is put, 10 microns of width, and two pneumatic control passages 2202 are at an angle with the symmetrical chiasma of fluid pipe 1101.Pneumatic control
Tubing giving sufficient strength is connected with the external world, when injecting gases at high pressure into pneumatic control pipeline 2202, the elastic membrane under pneumatic control pipeline
It can downwards bend, extrude the fluid pipe below elastic membrane, when removing gases at high pressure, elastic membrane is replied.But pipeline
2201 because width is smaller, and elastic membrane deformation is not enough to extruding lower section fluid pipe.
Principle and action relationships explanation：
The size cell granulations of well mixed distribution separates when the chip can be to injection, makes to be relatively large in diameter cell in liquid
The flowing of flow tube road center.Principle is, when injecting gases at high pressure, the elastic membrane of pipeline lower zone to pneumatic control pipeline 2202
Bent to fluid pipe, control injection gas pressure, the elastic membrane of the lower section of pneumatic control pipeline 2202 is bent to fluid pipe,
But keep certain interval with fluid pipe bottom, it is allowed to which liquid and diameter circulate compared with cellule, but can stop in cell suspension
Major diameter cell, now the pipeline 2201 of the junction of two pneumatic control pipeline 2202 due to diameter it is smaller, lower section elastic membrane bending
Degree is low, can allow for the liquid in the fluid pipe of lower section and various cells to pass through.Due to pneumatic control pipeline 2202 and liquid stream
At an angle, the cell being relatively large in diameter being trapped is along the edge of pneumatic control pipeline 1202 from fluid pipe two is lateral for pipeline
Centre is slided, the fluid pipe center below junction pipeline 2201, the barrier effect of pneumatic control pipeline is broken away from, with liquid stream stream
Go out, queue is formed in region shown in the B2 of fluid pipe center, and the less cell of diameter is by pneumatic control pipeline due to not stopped,
Still more it is evenly distributed in liquid stream pipeline, although there may also be the less cell of a small amount of diameter in B2 regions, leads to
Multiple structures of connecting are crossed, the less cell of a small amount of diameter can finally be excluded from liquid stream pipeline central region.
Embodiment 3 refers to Fig. 3, and the micro-fluidic chip is made up of three structure sheafs stacked on top of one another.Bottom is liquid stream layer,
Upper strata is pneumatic control layer, flexible film between two layers.There is fluid pipe 3101 in liquid stream layer, pipeline is wide 100 microns, and high 20 is micro-
There is interface at rice, both ends with being connected outside chip.There are above fluid pipe 3,101 two 10 microns of width in pneumatic control layer
Pneumatic control passage 3201 is connected with each other by a triangle Pneumatic pipe 3202, and triangle Pneumatic pipe 3202 is located at liquid stream
The middle upper part position of pipeline 3101, three sides are 60 microns, a symmetry axis and the medium line of fluid pipe 1101 of delta-shaped region
Overlap.Pneumatic control pipeline is connected with the external world, when injecting gases at high pressure, triangle pneumatic tube into pneumatic control pipeline 3201
Elastic membrane under road 3202 can bend downwards, extrude the fluid pipe below elastic membrane, when removing gases at high pressure, elasticity
Film is replied.But pipeline 3201, because width is smaller, elastic membrane deformation is not enough to extruding lower section fluid pipe.
Principle and action relationships explanation：
The size cell granulations of A3 regions mixed distributions separates when the chip can be to injection, makes to be relatively large in diameter cell and exists
Flow fluid pipe both sides.Principle is, when injecting gases at high pressure, the bullet of pipeline lower zone to triangle Pneumatic pipe 3202
Property film bent to fluid pipe, control injection gas pressure, make the elastic membrane of the lower section of triangle Pneumatic pipe 3202 to fluid flow tube
Road is bent, but keeps certain interval with fluid pipe bottom, it is allowed to which liquid and diameter circulate compared with cellule, but can stop that cell hangs
Major diameter cell in liquid, now two pneumatic control pipelines 3201 due to diameter it is smaller, lower section elastic membrane degree of crook is low, can
The liquid in the fluid pipe of lower section and various cells is allowed to pass through.Because the both sides of triangle Pneumatic pipe 3202 and liquid stream pipeline are in
Certain angle, the cell being relatively large in diameter being trapped break away along the edge of triangle Pneumatic pipe 3202 from fluid pipe mediad two
Dynamic, the fluid pipe below two pneumatic control pipelines 3201, i.e. fluid pipe both sides, the stop for breaking away from pneumatic control pipeline are made
With, flowed out with liquid stream, queue is formed in region shown in the B3 of fluid pipe both sides, and the less cell of diameter is not due to being controlled pneumatically
Tubing giving sufficient strength stops, is still more evenly distributed in liquid stream pipeline, although may also have a small amount of diameter less in B3 regions
Cell, but by multiple structures of connecting, the less cell of a small amount of diameter can finally be excluded from major diameter cell.
Embodiment 4 refers to Fig. 4 A ~ Fig. 4 B, and the micro-fluidic chip is made up of three structure sheafs stacked on top of one another.Bottom is liquid
Fluid layer, upper strata are pneumatic control layers, flexible film between two layers.There is fluid pipe 4101 in liquid stream layer, pipeline is wide 100 microns,
High 20 microns, there is interface at both ends with being connected outside chip.There are two width 100 in pneumatic control layer above fluid pipe 4101
The pneumatic control passage 4202 of micron is connected with each other, and there is the narrow pipeline 4201 of a width junction, positioned at fluid pipe 4101
Middle upper part position, 10 microns of width, two pneumatic control passages 4202 are at an angle with the symmetrical chiasma of fluid pipe 4101.
Pneumatic control pipeline is connected with the external world, when injecting gases at high pressure into pneumatic control pipeline 4202, under pneumatic control pipeline
Elastic membrane can be bent downwards, extrude the fluid pipe below elastic membrane, and when removing gases at high pressure, elastic membrane is replied.But
For pipeline 2201 because width is smaller, elastic membrane deformation is not enough to extruding lower section fluid pipe.
Principle and action relationships explanation：
The size cell granulations of well mixed distribution is separated and is enriched with when the chip can be to injection, makes to be relatively large in diameter carefully
Born of the same parents are in fluid pipe center, agglomerating flowing.Principle is, when injecting gases at high pressure to pneumatic control pipeline 4202, below pipeline
The elastic membrane in region is bent to fluid pipe, control injection gas pressure, makes pneumatic control pipeline 4202 and the pipeline of junction
The elastic membrane of 2201 lower sections is bent to fluid pipe, but keeps certain interval with fluid pipe bottom, it is allowed to liquid and diameter compared with
Cellule circulates, but can stop the major diameter cell in cell suspension.Because pneumatic control pipeline 2202 and liquid stream pipeline are in one
Determine angle, the cell being relatively large in diameter being trapped slides along the edge of pneumatic control pipeline 1202 from the lateral center of fluid pipe two,
Fluid pipe center aggregation below junction pipeline 2201, as B4 regions show in Fig. 4 A, then removes pneumatic control pipe and arrives
Middle air pressure, the large granule cells of aggregation are just all discharged into fluid pipe center and flowing are enriched with finite region, such as schemed
In 4B shown in C4 regions, and the less cell of diameter by pneumatic control pipeline due to not stopped, still more uniform to be distributed
In liquid stream pipeline, although may also have the less cell of a small amount of diameter in B2 regions, by multiple structures of connecting, most
The less cell of a small amount of diameter can be excluded from the major diameter cell of aggregation eventually.
Obviously, microfluidic chip structure of the invention is simple, easily fabricated, is easy to use, and can realize simultaneously different straight
Enrichment, the separation of footpath particulate matter, and stable work in work, are not easy to fail because blocking, have wide application prospect.
It should be noted that herein, such as first and second or the like relational terms are used merely to a reality
Body or operation make a distinction with another entity or operation, and not necessarily require or imply and deposited between these entities or operation
In any this actual relation or order.Moreover, term " comprising ", "comprising" or its any other variant are intended to
Nonexcludability includes, so that process, method, article or equipment including a series of elements not only will including those
Element, but also the other element including being not expressly set out, or it is this process, method, article or equipment also to include
Intrinsic key element.In the absence of more restrictions, the key element limited by sentence "including a ...", it is not excluded that
Other identical element also be present in process, method, article or equipment including the key element.
Described above is only the embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (8)
- A kind of 1. micro-fluidic chip, it is characterised in that including：Liquid stream layer, comprising fluid course, to make the suspension of the particulate matter comprising different-diameter and carrier fluid simultaneously in the chip Interior circulation, also, the suspension and carrier fluid are in the fluid course interior edge direction layer distributed vertical with fluid flow direction；Layers of elastomeric film, include the elastic membrane being covered on the fluid course；Pneumatic control layer, comprising one or more pneumatic control pipeline, to drive the regional area of the elastic membrane to produce trend The deformation and/or displacement of the fluid course, so that in the elastic membrane and liquid stream in the first area of the fluid course Being formed between passage bottom face can stop that particle diameter is more than the larger particles of a setting value and particle diameter is less than the setting value The gap that smaller particle thing and liquid pass through, at the same in the fluid course formed will can be stopped by the gap it is larger Particulate matter imports the guiding structural of the second area of the fluid course,The first area and second area are respectively distributed in the region that the suspension and carrier fluid flow through；The pneumatic control pipeline includes caliber and is less than described select more than thicker the pneumatic control pipeline and caliber of a set point value The thinner pneumatic control pipeline of value, the thicker pneumatic control pipeline and thinner pneumatic control pipeline respectively with the fluid course First area and second area intersect；The thicker pneumatic control pipeline gradually tilts from one first point to a second point, also, in the fluid course On suspension flow direction, first point of upstream for being distributed in second point, and described first point is distributed in first area, second Point is distributed in the intersection of first area and second area,When the fluid of injection setting pressure into the thicker pneumatic control pipeline, the thicker pneumatic control pipeline drives institute State elastic membrane and produce the deformation and/or displacement for tending to the fluid course bottom face, so that in described in the first area The gap and the guiding structural are formed between elastic membrane and the fluid course simultaneously.
- 2. micro-fluidic chip according to claim 1, it is characterised in that the suspension and carrier fluid are in the fluid course Flow in the same direction.
- 3. according to the micro-fluidic chip any one of claim 1-2, it is characterised in that the thicker pneumatic control pipeline It is interconnected with the thinner pneumatic control pipeline.
- 4. micro-fluidic chip according to claim 3, it is characterised in that the thicker pneumatic control pipeline with it is described thinner Pneumatic control pipeline is continuously coupled.
- 5. the micro-fluidic chip according to claim 1 or 4, it is characterised in that on the thicker pneumatic control pipeline with it is described The part that fluid course intersects has arc line shaped or linear profile.
- 6. the micro-fluidic chip according to claim 1 or 4, it is characterised in that the pneumatic control pipeline includes being interconnected And a continuously coupled thicker pneumatic control pipeline and a thinner pneumatic control pipeline, the thicker pneumatic control pipeline is from described By and second area of the thinner pneumatic control pipeline out of described fluid course above first area in fluid course Top passes through.
- 7. the micro-fluidic chip according to claim 1 or 4, it is characterised in that the pneumatic control pipeline includes being interconnected And one first thicker pneumatic control pipeline, a thinner pneumatic control pipeline and the one second thicker pneumatic control pipe being sequentially connected Road, the first, second thicker pneumatic control pipeline respectively above two first areas out of described fluid course by, and The thinner pneumatic control pipeline is above the second area out of described fluid course by the way that the second area is distributed in this Between two first areas.
- 8. the micro-fluidic chip according to claim 1 or 4, it is characterised in that the pneumatic control pipeline includes being interconnected And one first thinner pneumatic control pipeline, a thicker pneumatic control pipeline and the one second thinner pneumatic control pipe being sequentially connected Road, the first, second thinner pneumatic control pipeline respectively above two second areas out of described fluid course by, and The thicker pneumatic control pipeline is above the first area out of described fluid course by the way that the first area is distributed in this Between two second areas.
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| CN103834554A (en) * | 2012-11-28 | 2014-06-04 | 中国科学院苏州纳米技术与纳米仿生研究所 | Microfluidic microbial culture detection chip |
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| US7763453B2 (en) * | 2005-11-30 | 2010-07-27 | Micronics, Inc. | Microfluidic mixing and analytic apparatus |
| ITTO20100068U1 (en) * | 2010-04-20 | 2011-10-21 | Eltek Spa | MICROFLUID AND / OR EQUIPMENT DEVICES FOR MICROFLUID DEVICES |
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| CN103834554A (en) * | 2012-11-28 | 2014-06-04 | 中国科学院苏州纳米技术与纳米仿生研究所 | Microfluidic microbial culture detection chip |
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