CN1055117C - Microbe, microbial product quick rotting agent and its preparing method - Google Patents
Microbe, microbial product quick rotting agent and its preparing method Download PDFInfo
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Abstract
The present invention relates to preparation of microorganisms and a product thereof. The product is formed by using a 301 bacterium agent as a basis which is added with seven kinds of microorganisms and five kinds of plant growth regulating agents and is shortened as an eight-into-one microorganism quick-putrefying agent. The eight-into-one microorganism quick-putrefying agent can rapidly putrefy crop straws into a fertilizer, the putrefying time is shortened by 1/3 of that of the 301 bacterium agent, and both the volume and the dosage are reduced by 4/5 of that of the 301 bacterium agent. The eight-into-one microorganism quick-putrefying agent has the advantages of fertilizer cultivation, disease prevention, phosphorus and potassium decomposition, nitrogen fixation and soil improvement after being used for composting and fertilization in soil; furthermore, the eight-into-one microorganism quick-putrefying agent can also decompose alkali, pesticides and fertilizer residues in the soil and toxin in agricultural products, so crops can have the advantages of high yield, high quality and nuisance free. The quick-putrefying agent also has the advantages of wide raw materials, low price, simple preparation and extremely easy popularization in rural areas.
Description
The invention belongs to the preparation of microorganism and products thereof.
Crop material is a kind of Biological resources of preciousness, China produces more than 500,000,000 ton per year, except that directly for people utilize, to the most economical rational purposes of remainder be do poultry, the fowl feed " is crossed also field of abdomen ", but the restriction that planned by animal husbandry development, also can not make whole residue stalks " cross abdomen ", a large amount of residue stalks account for presses many arable land, blocks the traffic contaminate environment, and be big steady suffering from of present rural fire, all wish to eliminate these public hazards throughout the country; Meanwhile, most organic wretched insufficiencies in 14.3 hundred million mu of arable lands of China, people are devoted to utilize microorganism with the rotten also field of stalk heap for many years, expectation increases soil nutrient and organic matter, improve the physicochemical property of soil, reach and combine the use of land with the nurturing of land, make agriculture production realize high yield, stable yields, high-quality, low cost, oligosaprobic purpose.Far away from 1940, Japanese microbiologist's this feel of island also utilized bacterium, actinomycetes and yeast to form composite fungus agent-enzymatic microorganism, introducing China in 1994, just use in the Weifang City at present, it is better to pile rotten effect, only its manufacturing technology complexity, cost is higher, promotes to be subjected to certain limitation.
The objective of the invention is to develop a kind of good and cheap, pile rotten effect bacterial classification preferably, and develop a kind of microniological proudcts, its volume is little, bacteria containing amount is high, is convenient to transportation and carries short quick rotting agent of rotten time of heap.
Content of the present invention is:
A kind of microorganism is characterized in that " 301 " bacterial classification is thermophilic sporotrichum (Sporotrichum Thermophile), is preserved in CGMCC, preserving number 0186 on December 22nd, 1992.
The source and the microbiological property of " 301 " bacterial classification:
" 301 " bacterial classification is the extremely strong mould of a kind of saprophytic property, is to use Co
60Gamma-rays 10 Wan Gerui shine thermophilic sporotrichum (Sporotrichum Thermophile), form through the separation and Culture seed selection, name to be " 301 " bacterial classification.Position in the classification of biology system is: a new bacterial strain in the Mycophyta deuteromycetes Moniliales Moniliaceae Sporotrichum thermophilic sporotrichum strain." 301 " bacterial classification bacterium colony on nutrient agar often is flannelette blanket shape, flocculence or particulate state, and color is a white or light yellow.Mycelia is than former female slightly weak point of planting, and width is 3～10 μ m, and a lot of branches are arranged, and in a tubular form, every joint is a cell.In the mycelia clump, generative hyphae is arranged, above living sporophore estranged, the stalk on the many conidiums of adnation, come in every shape.The spore of " 301 " bacterial classification is tawny, but slightly more shallow than original seed.Conidium is not of uniform size, is generally 2.8 * 4.0 μ m, but a small amount of megaspore is arranged, and is generally 5.0 * 8.0 μ m, and indivedual megaspores are 5.0 * 10 μ m.But do not find the sexual process of " 301 " bacterial classification at present as yet.It can also can be bred with conidium with the mycelia breeding, can breed a generation in general 24 hours." 301 " bacterial classification is a kind of high temperature modification bacterial classification, but adaptability is very strong, 30 ℃ of minimum requirements, and optimal temperature is 43～45 ℃, is up to 60～75 ℃, but also can be all not dead in the time of subzero 20 ℃.The moisture-proof power of mycelia is stronger, requires moisture content in medium 65～75%, and it is a kind of good gas fungi, and oxygen requirement is big, the normal CO of mycelia
2Content is 0.03%, and vegetative stage does not need illumination, is suitable for neutrality to the slight alkalinity environment in cultivation, and the pH value is 6.7～6.9, and optimum carbon nitrogen ratio is 30～40: 1.Can preservation in freezing or dry environment.
A kind of microbial product quick rotting agent, it is characterized in that based on " 301 " bacterial classification, add that 7 kinds of high-effective microorganisms are amylomyces, thermophilic actinomycete, organophosphorus bacterium, inorganic phosphorus bacteria, vinelandii, potassium bacterium and yeast, 5 kinds of growth regulators are iron protochloride, trimagnesium phosphate, saltpetre, cyanamid and Sodium Metabisulphate 65.
The prescription of quick rotting agent is (weight percent):
(1) " 301 " bacterial classification 50～55%
(2) amylomyces 4～7%
(3) thermophilic actinomycete 20～30%
(4) the organophosphorus bacterium 5～7%
(5) inorganic phosphorus bacteria 5～7%
(6) vinelandii 1～5%
(7) the potassium bacterium 5～7%
(8) yeast 4～6%
(9) 5 kinds of growth regulators 0.01%
The content ratio of various conditioning agents inside is in 5 kinds of growth regulators of quick rotting agent: (weight ratio)
Iron protochloride 69%
Trimagnesium phosphate 8.5%
Saltpetre 3.5%
Cyanamid 16%
Sodium Metabisulphate 65 3%
A kind of manufacture method of quick rotting agent comprises spawn culture and compost,
(1) technological process of " 301 " bacterial classification production:
" 301 " spawn culture three phases, existing division is as follows:
(1) first class inoculum (the female kind) is cultivated: first class inoculum expands numerous by the most original inclined-plane first class inoculum, a slant strains can expand and connects 30～40 first class inoculums, and substratum is PDA or wort, and pH value is 6.7～6.9, culture temperature is 43～45 ℃, and incubation time is 72～96 hours.
(2) second class inoculum is cultivated: first class inoculum is inoculated on the wheat substratum, the making method of wheat substratum is: take by weighing wheat 5kg earlier, in clear water, soaked 12 hours, putting into boiling water again boiled 30 minutes, waterloging out drains, and dries in the air in the ventilation to anhydrous, adds lime carbonate 15g again, gypsum 60g, wheat mixed thoroughly get final product wheat Cans packing, pH value 6.7～6.9, an one-level kind can connect two bottles, culture temperature is 43～45 ℃, and incubation time 6～8 days is in order to enlarge second class inoculum rapidly, available second class inoculum expands once numerous, and general one bottle of expansion connects 20 bottles.
(3) three-class strain is cultivated: produce for three grades of " 301 " bacterial classification and plant, be called for short " 301 " microbial inoculum, system makes carrier with cotton seed hulls before nineteen ninety-five, system makes carrier with dregs of beans or cotton dregs at present, big because of producing expense, generally all without autoclaving, can carry out on sterile concrete floor, operation steps is as follows:
A, take by weighing cotton dregs (or dregs of beans) 88kg, Semen Maydis powder 5kg, wheat bran 5kg, brown sugar 1kg, milk powder 1kg, 10 bottles in original seed, water 50kg.
B, earlier that brown sugar and milk powder is soluble in water, then this solution is added in cotton dregs Semen Maydis powder and the wheat bran, the limit adds the waterside and stirs, till 50kg water adds, material is mixed, divided three layers of composting, 60 centimetres of total thickness, on one, two layer, respectively spread 2 bottles in original seed, floating on the 3rd layer of top with spade, spread 6 bottles in original seed, and bacterial classification is pressed in the material with plank or spade, stamp some ventilation holes, cover straw screen or mat and preserve moisture, be incubated, through fermentation in 4～5 days, white hypha was covered with charge level, have sweet, sour, fragrance to get final product, through dried and crushed " 301 " microbial inoculum.
(2) source of amylomyces and biological characteristics thereof:
The source of amylomyces is identical with " 301 " bacterial classification, and it is the difference choosing system after the radiation, and formalness and " 301 " bacterial classification are approximate, on nutrient agar, be the flannelette blanket shape, color is a yellow-white, and mycelia is shorter than " 301 " bacterial classification, with " 301 " bacterial classification have 3 different: (1) does not have megaspore; (2) in 1～3 grade of cultivation, fragrant and sweet flavor is arranged; (3) mycelial growth is than a little less than " 301 " microbial inoculum.Amylomyces can be secreted saccharifying enzyme, with the further hydrolysis of polysaccharide, for microbial nutrition.The operation steps that the amylomyces three-class strain is produced is as follows:
(1) takes by weighing cotton dregs (or dregs of beans) 88kg, Semen Maydis powder 5kg, wheat bran 5kg, brown sugar 1kg, milk powder 1kg, 10 bottles in original seed, water 50kg.
(2) earlier brown sugar and milk powder are dissolved with less water, add cotton dregs, Semen Maydis powder and wheat bran again, water is filled up 5 0kg, in the basin alms bowl or at the clean water mire, pile up then, on one, two layer, respectively spread 2 bottles in original seed, cover the 3rd layer after, floating with spade, on heap, spread 6 bottles of original seeds then, and bacterial classification is pressed in the material with plank or spade, stamp some ventilation holes, cover straw screen or mat, through fermentation in 5～7 days, white hypha was covered with charge level, have fragrant and sweet flavor to get final product, through dried and crushed the saccharification microbial inoculum.
(3) substratum of thermophilic actinomycete:
(1) first class inoculum substratum:
Cottonseed cake powder 15 gram potassium primary phosphates 0.2 gram water 1000ml
Glucose 20 gram sodium-chlor 4 gram peptones 5 grams
Lime carbonate 10 gram sulfate of ammoniacs 2.5 gram agar 20 grams
The pH value is 72～7.4
(2) second class inoculum substratum:
Cottonseed cake powder 85kg brown sugar 1kg wheat bran 5kg
Calcium superphosphate 4kg Semen Maydis powder 5kg water 50kg
(3) three-class strain substratum:
Wheat bran 1000kg original seed 50kg brown sugar 20kg Guizhou calcium magnesium phosphorus 40kg water 200～240kg
(4) vinelandii (Ashby) substratum:
KH
2PO
4 0.2g CaCO
3?5g MgSO
4·7H
2O 0.2g
C
6H
12O
6?10g NaCl 0.2g CaSO
4·2H
2O 0.1g
H
2O 1000ml agar 18～20 grams
PH value 7.0
(5) organophosphorus bacteria culture medium:
C
6H
12O
6?10g MnSO
4·4H
2O 0.03g (NH
4)
2SO
4?0.5g
NaCl 0.3g MgSO
4·7H
2O 0.3g CaCO
3 5g
KCl 0.3g FeSO
47H
2O 0.03g Yelkin TTS 0.2g
H
2O 1000ml agar 18～20 grams
PH value 7～7.5
(6) inorganic phosphorus bacteria substratum:
C
6H
12O
6 10g MnSO
4·4H
2O 0.03g NaCl 0.3g
(NH
4)
2SO
4?0.5g MgSO
4.7H
2O 0.3g Ca
3(PO
4)
2 5g
H
2O 1000ml FeSO
47H
2O 0.03g agar 18～20 grams
pH 7.0～7.5
(7) potassium bacteria culture medium:
Potato 200g C
6H
12O
620g MgSO
47H
2O 0.3g
NaCl 0.3g KCl 0.3g CaCO
3 5g
H
2O 1000ml agar 18～20 grams
pH 7.0～7.5
(8) microzyme culture medium (summary)
More than five bacterial classifications oneself 1～3 grade of substratum is all arranged, must deposit by strict Dan Fandan, avoid mutual pollution.Different ratios adds in certain sequence when synthetic.The chemical additive consumption too much influences microbial survival, and the very few regulating effect that do not have of consumption must stir gently with spade when preparation, must not thump, and more can not beat with waddy, in order to avoid set off an explosion.
Advantage of the present invention:
(1) contain that bacterium is many, volume is little, heap is rotten fast;
(2) compost nutriment in a fertilizer height, conventional compost organic matter is 15～25%; Be 35～59 only with single " 301 " microbial inoculum compost organic matter; With quick rotting agent compost organic matter 45～60%.Also containing 8～10% nitrogen, phosphorus, potassium element in organic matter, though their content stable in properties less can all be absorbed by crop, is about ten times of the fat branch of generalization; High-effective microorganism in the quick rotting agent can both constantly be bred in compost or after being manured into soil, and the nitrogen in the energy fixed air can be converted into the utilizable phosphorus of crop to insoluble phosphorus in the soil, improves the plain nutrition of crop phosphorus.
(3) also contain in the quick rotting agent and stimulate the growth hormone of plant growth material, make that crop yield is huge to be increased, quality improving, we test on 18 planting fruit-trees, vegetable crop and have obtained peculiar effect in the recent period, and single-dose application is sustainable effectively.
(4) soil-borne disease can be killed or suppress to the eight-in-one quick rotting agent, as root rotof flax, banded sclerotial blight, canker of apple fruit, the late blight of potato, bacterial wilt of ginger, watermelon continuous cropping disease, asparagus stem wilt etc.
(5) can decompose soil alkaline and decomposition agricultural-food residual hazard,, reach the national feeding standard of 400ppm/kg basically as the cotton dregs residual hazard is reduced to 351ppm/kg by 1500ppm/kg.
(6) with " 301 " microbial inoculum compost, once can pile 1000～2000 kilograms of stalks, need with the grave heap, the centre also need be turned over and be smash once; Use the quick rotting agent compost, once can pile stalk 1000～250000kg, need not turn over and smash, cover (rickle need cover with soil) without earth.
Embodiment one:
Pile little fertilizer with the eight-in-one quick rotting agent.Take by weighing 1000 kilograms of stalks, be layered on the ground, water fully irrigates, and this is the compost key of success.Wide 1.6～the 2.0m in the bottom of compost, long 5～6m successively piles up with the branch grass that will wet.The thick 60cm of the first layer grass, after flattening with branch, 0.2 kilogram of quick rotting agent winnows, spread 1 kilogram of carbon-nitrogen ratio conditioning agent (urea), according to the preceding method heap second layer, spread bacterial classification and urea then, up pile wet stalk subsequently again, thick 30～40cm, 0.1 kilogram of the quick rotting agent of winnowing again, 0.5 kilogram in urea, pat with branch gently at the top, make in quick rotting agent and urea " implantation " grass, get the mudding heap then on the spot, can become thoroughly decomposed in general 20 days.If make the carbon-nitrogen ratio conditioning agent with human and animal excreta, its consumption be stalk heavy 10%.
Embodiment two:
Pile big compost with the eight-in-one quick rotting agent.Its principle and the little compost of heap are in full accord, but the stalk that raft is used is more, and 2000～250000 kilograms, to pile widely 3～4 meters, the ratio of quick rotting agent and carbon-nitrogen ratio conditioning agent also is consistent.But because of heap raft quick rotting agent amount is few, the utmost point is not easy to spread even, preferably the quick rotting agent breeding is enlarged 1～2 time.The concrete practice is: take by weighing each 25 kilograms in Semen Maydis powder, wheat bran and water, mix 1～1.5 kilogram of quick rotting agent, in cylinder or concrete floor top fermentation 24～48 hours, white hypha occurs and have fragrant and sweet flavor to get final product.Because of the bacterial classification amount big, the level of compost can be more than three layers, every layer also can suitably approach, but the top is certain with wet broken grass envelope heap, more than the thick 30cm, use the branch compacting, both, also need not turn over and smash, be preferably in heap top cover plot film without the mudding heap, in case heavy rain or heavy rain water heap, excess moisture influence fermentation.Heap top cover grass may rot insufficient, can push aside to give over to next compost and use.
Fruit tree and booth vegetable belong to expensive high benefit operation, need a lot of high-quality fertilizers, general each canopy needs chicken manure 3 sides, if an amount of stalk of adding and 1.5 side's chicken manures stack retting together also can obtain high-quality fertilizer, also available quick rotting agent ferments chicken manure, cottonseed cake and stalk together, adds an amount of chemical fertilizer and trace element then and makes all kinds of organic-biological compound fertilizers.
Claims (4)
1, a kind of microbial product quick rotting agent, it is characterized in that based on " 301 " bacterial classification, " 301 " bacterial classification is thermophilic sporotrichum Sporotrichum Thermophile, be preserved in CGMCC, preserving number 0186, add that 7 kinds of high-effective microorganisms are amylomyces, thermophilic actinomycete, organophosphorus bacterium, inorganic phosphorus bacteria, vinelandii, potassium bacterium and yeast, 5 kinds of growth regulators are iron protochloride, trimagnesium phosphate, saltpetre, cyanamid and Sodium Metabisulphate 65.
2, quick rotting agent as claimed in claim 1 is characterized in that its prescription is (weight percent):
(1) " 301 " bacterial classification 50-55%
(2) amylomyces 4-7%
(3) thermophilic actinomycete 20-30%
(4) organophosphorus bacterium 5-7%
(5) inorganic phosphorus bacteria 5-7%
(6) vinelandii 1-5%
(7) potassium bacterium 5-7%
(8) yeast 4-6%
(9) 5 kinds of growth regulators 0.01%
3, quick rotting agent as claimed in claim 2 is characterized in that the percentage composition of various conditioning agents inside in 5 kinds of growth regulators is: (weight ratio)
Iron protochloride 69%
Trimagnesium phosphate 8.5%
Saltpetre 3.5%
Cyanamid 16%
Sodium Metabisulphate 65 3%
4, a kind of manufacture method of quick rotting agent comprises spawn culture and compost, it is characterized in that:
(1) technological process of " 301 " bacterial classification production:
" 301 " spawn culture three phases, existing division is as follows:
(1) first class inoculum (the female kind) is cultivated: first class inoculum expands numerous by the most original inclined-plane first class inoculum, slant strains can expand and meets 30-40 and prop up first class inoculum, and substratum is PDA or wort, and pH value is 6.7-6.9, culture temperature is 43-45 ℃, and incubation time is 72-96 hour;
(2) second class inoculum is cultivated: first class inoculum is inoculated on the wheat substratum, the making method of wheat substratum is: take by weighing wheat 5kg earlier, in clear water, soaked 12 hours, putting into boiling water again boiled 30 minutes, waterloging out drains, and dries in the air in the ventilation to anhydrous, adds lime carbonate 15g again, gypsum 60g, wheat mixed thoroughly get final product wheat Cans packing, pH value 6.7-6.9, an one-level kind can connect two bottles, culture temperature is 43-45 ℃, incubation time 6-8 days, in order to enlarge second class inoculum rapidly, available second class inoculum expands once numerous, and general one bottle of expansion connects 20 bottles;
(3) three-class strain is cultivated: produce for three grades of " 301 " bacterial classification and plant, be called for short " 301 " microbial inoculum system and make carrier with dregs of beans or cotton dregs, can carry out on sterile concrete floor, operation steps is as follows:
A, take by weighing cotton dregs (or dregs of beans) 88kg, Semen Maydis powder 5kg, wheat bran 5kg, brown sugar 1kg, milk powder 1kg, 10 bottles in original seed, water 50kg;
B, earlier that brown sugar and milk powder is soluble in water, then this solution is added in cotton dregs Semen Maydis powder and the wheat bran, the limit adds the waterside and stirs, till 50kg water adds, material is mixed, divided three layers of composting, 60 centimetres of total thickness, on one, two layer, respectively spread 2 bottles in original seed, floating on the 3rd layer of top with spade, spread 6 bottles in original seed, and bacterial classification is pressed in the material with plank or spade, stamp some ventilation holes, cover straw screen or mat and preserve moisture, be incubated, through fermentation in 4-5 days, white hypha was covered with charge level, have sweet, sour, fragrance to get final product, through dried and crushed " 301 " microbial inoculum;
(2) operation steps of amylomyces three-class strain production is as follows:
(1) takes by weighing cotton dregs (or dregs of beans) 88kg, Semen Maydis powder 5kg, wheat bran 5kg, brown sugar 1kg, milk powder 1kg, 10 bottles in original seed, water 50kg;
(2) earlier brown sugar and milk powder are dissolved with less water, add cotton dregs, Semen Maydis powder and wheat bran again, water is filled up 50kg, in the basin alms bowl or at the clean water mire, pile up then, on one, two layer, respectively spread 2 bottles in original seed, cover the 3rd layer after, floating with spade, on heap, spread 6 bottles of original seeds then, and bacterial classification is pressed in the material with plank or spade, stamp some ventilation holes, cover straw screen or mat, through fermentation in 5-7 days, white hypha was covered with charge level, have fragrant and sweet flavor to get final product, through dried and crushed the saccharification microbial inoculum;
(3) substratum of thermophilic actinomycete:
(1) first class inoculum substratum:
Cottonseed cake powder 15 gram potassium primary phosphates 0.2 gram water 1000ml
Glucose 20 gram sodium-chlor 4 gram peptones 5 grams
Lime carbonate 10 gram sulfate of ammoniacs 2.5 gram agar 20 grams
The pH value is 7.2-7.4
(2) second class inoculum substratum
Cottonseed cake powder 85kg brown sugar 1kg wheat bran 5kg
Calcium superphosphate 4kg Semen Maydis powder 5kg water 50kg
(3) three-class strain substratum
Wheat bran 1000kg original seed 50kg brown sugar 20kg
Guizhou calcium magnesium phosphorus 40kg water 200-240kg
(4) vinelandii (Ashby) substratum
KH
2PO
4 0.2g CaCO
3?5g MgSO
4·7H
2O 0.2g
C
6H
12O
610g NaCl 0.2g CaSO
4·2H
2O 0.1g
H
2O 1000ml agar 18-20 gram
PH value 7.0 (five) organophosphorus bacteria culture mediums
C
6H
12O
6 10g MnSO
4·4H
2O 0.03g (NH
4)
2SO
4?0.5g
NaCl 0.3g MgSO
4·7H
2O 0.3g CaCO
3 5g
KCl 0.3g FeSO
47H
2O 0.03g Yelkin TTS 0.2g
H
2O 1000ml agar 18-20 gram
PH value 7-7.5 (six) inorganic phosphorus bacteria substratum
C
6H
12O
6 10g MnSO
4·4H
2O 0.03g NaCl 0.3g
(NH
4)
2SO
4 0.5g MgSO
4·7H
2O 0.3g Ca
3(PO
4)
2 5g
H
2O 1000ml FeSO
47H
2O 0.03g agar 18-20g
PH value 7.0-7.5 (seven) potassium bacteria culture medium
Potato 200g C
6H
12O
620g MgSO
47H
2O 0.3g
NaCl 0.3g KCl 0.3g CaCO
3 5g
H
2O 1000ml agar 18-20 gram
PH value 7.0-7.5
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN97116992A CN1055117C (en) | 1997-10-10 | 1997-10-10 | Microbe, microbial product quick rotting agent and its preparing method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN97116992A CN1055117C (en) | 1997-10-10 | 1997-10-10 | Microbe, microbial product quick rotting agent and its preparing method |
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| Publication Number | Publication Date |
|---|---|
| CN1180103A CN1180103A (en) | 1998-04-29 |
| CN1055117C true CN1055117C (en) | 2000-08-02 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN97116992A Expired - Fee Related CN1055117C (en) | 1997-10-10 | 1997-10-10 | Microbe, microbial product quick rotting agent and its preparing method |
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| CN (1) | CN1055117C (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015170961A1 (en) * | 2014-05-08 | 2015-11-12 | Malaysian Palm Oil Board | A composition to biologically control ganoderma disease and method of making thereof |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1118564C (en) * | 1998-12-25 | 2003-08-20 | 李雅君 | Micro-organism, micro-organism products, high-efficiency organic and inorganic micro-organism compound fertilizer |
| CN100425577C (en) * | 2003-04-10 | 2008-10-15 | 周清易 | Technology for manufacturing granular bio-fertilizer utilizing organic waste by granulating firstly and fermenting secondly |
| CN102559549B (en) * | 2011-12-27 | 2013-11-06 | 兰州大学 | Arid desert halophilic-basiphilic microbial agent and preparation method and application thereof |
| CN104272978B (en) * | 2014-10-30 | 2016-07-06 | 湖南省宇秀生物科技有限公司 | A kind of Pleurotus eryngii solid spawn liquefaction process |
| CN105036823A (en) * | 2015-07-24 | 2015-11-11 | 潍坊友容实业有限公司 | Thermophilic sporotrichum decomposition accelerator and application of thermophilic sporotrichum in saline-alkali soil straw decaying soil improvement |
| CN107827646A (en) * | 2017-11-16 | 2018-03-23 | 何建国 | One kind is loosened the soil biological organic fertilizer |
| CN107935781A (en) * | 2017-12-18 | 2018-04-20 | 何建国 | A kind of alkaline land modifying agent and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1078491A (en) * | 1993-02-09 | 1993-11-17 | 中国农业科学院 | A kind of method of quick rotting straws of crops and the microorganism of use thereof |
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1997
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1078491A (en) * | 1993-02-09 | 1993-11-17 | 中国农业科学院 | A kind of method of quick rotting straws of crops and the microorganism of use thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015170961A1 (en) * | 2014-05-08 | 2015-11-12 | Malaysian Palm Oil Board | A composition to biologically control ganoderma disease and method of making thereof |
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| Publication number | Publication date |
|---|---|
| CN1180103A (en) | 1998-04-29 |
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