CN105510109B - Blood sample sampling pretreatment device and its method - Google Patents
Blood sample sampling pretreatment device and its method Download PDFInfo
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- CN105510109B CN105510109B CN201510867804.6A CN201510867804A CN105510109B CN 105510109 B CN105510109 B CN 105510109B CN 201510867804 A CN201510867804 A CN 201510867804A CN 105510109 B CN105510109 B CN 105510109B
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- suction pipe
- blood sample
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
- G01N2001/386—Other diluting or mixing processes
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The present invention provides a kind of blood sample sampling pretreatment device and its methods, device includes blood sampling portion and sample mixing unit, blood sampling portion includes suction pipe and elastomer, elastic wall is equipped with compression and is open, the junction of compression opening and suction pipe is equipped with a sealing ring, and the both sides of sealing ring are equipped with the adhesive layer of seal compression opening and the junction of suction pipe；Sample mixing unit includes the mixing vessel for accommodating suction pipe, lower half in mixing vessel is equipped with the diaphragm seal punctured for the end of suction pipe, diaphragm seal and the wall of mixing vessel are formed with closed treatment fluid and house layer, the bottom of mixing vessel is additionally provided with houses the drain unit that layer is connected to treatment fluid, and the top of mixing vessel is equipped with the vessel port of size corresponding with the caliber of suction pipe.The apparatus structure is simple, fast and easy, and which obviate blood samples in mixed liquor manufacturing process and sample treatment solution to be in direct contact with external environment, reduces contaminated probability and biological safety height.
Description
Technical field
The present invention relates to the field of medical instrument technology, more particularly to a kind of blood sample sampling pretreatment device and its side
Method.
Background technology
Existing external diagnosis reagent micro blood collection of specimens, mostly use pipette and centrifuge tube carry out blood sample and
The mixing of sample treatment solution, but the hybrid mode can cause blood sample and sample treatment solution directly to be connect with external environment
It touches, and time of contact is long, is polluted so as to cause sample, biological safety is relatively low.
Invention content
The purpose of the present invention is to overcome the above shortcomings and to provide a kind of blood sample sampling pretreatment device and its method,
It by siphon principle, avoids blood sample and sample treatment solution and is in direct contact with external environment, when mixing and external environment
It is contaminated to effectively prevent sample for isolation.
To achieve the above object, there is provided a kind of blood samples to sample pretreatment dress for technical solution of the invention
It sets, including：The blood sampling portion being detachably connected and sample mixing unit；The blood sampling portion includes a hollow suction pipe,
One end of the suction pipe is communicated with elastomer, and the inside of the elastomer is enclosed with the pressure of the elastic wall of the resilient elastomer
Contracting space, the elastic wall are equipped with compression and are open, and the compression opening and the junction of the suction pipe are equipped with a sealing ring, and
The both sides of the sealing ring are equipped with the adhesive layer for sealing the compression opening and the junction of the suction pipe；The sample mixing unit
Include the mixing vessel for accommodating suction pipe, the lower half in the mixing vessel punctures close equipped with the end for the suction pipe
The wall of sealer, the diaphragm seal and the mixing vessel is formed with closed treatment fluid and houses layer, the bottom of the mixing vessel
Portion is additionally provided with houses the drain unit that layer is connected to the treatment fluid, and the top of the mixing vessel is equipped with the pipe with the suction pipe
Diameter corresponds to the vessel port of size, so that the elastomer is fixed in the vessel port.
Preferably, baffle is equipped in the mixing vessel of vessel port, the baffle is equipped with the corresponding suction
The connecting hole of pipe caliber, the baffle and the wall of the mixing vessel are formed with installation space, are equipped in the installation space
Guiding piece, the guiding piece middle part are equipped with the guiding groove of the corresponding suction pipe outer diameter.
Preferably, the baffle connecting hole peripheral wall is equipped with the elastic packaging flexible glue being arranged towards mixing vessel bottom direction
Body.
Preferably, the elastic packaging flexible glue body have hollow cyclic annular flexible glue ontology, and with the flexible glue ontology phase
Even and the tapered centrum interconnecting piece of volume.
Preferably, the centrum interconnecting piece includes the multiple arcs flap valve for contradicting setting.
Preferably, the junction of the elastomer and the suction pipe is formed with the L-shaped card slot in cross section, and the container is opened
Mouth is contradicted with the card slot and is connected.
Preferably, the sample mixing unit further includes the protection cap of sealing container opening, and the protection cap has collecting post
State the guard space of elastomer.
Preferably, the protection cap has the first lid of receiving elastomer, and be connected with first lid and
The second lid extended towards the mixing vessel bottom direction, the second lid ring set is on the side wall of mixing vessel, and institute
It states the second lid and is equipped with groove ring, the mixing vessel respective slot ring position is equipped with bulge loop.
Preferably, there is first lid the first lid mouth of corresponding vessel port, the first lid mouth to be equipped in
The easy abscission ring that portion is collapsed, the easy abscission ring size setting corresponding with the tube wall of the suction pipe, the easy abscission ring are equipped with rubber finger sleeve,
The opening plaster of the rubber finger sleeve is together on the easy abscission ring.
A kind of blood sample sampling preprocess method, includes the following steps：1) suction pipe is placed in blood sample by blood sampling
Middle carry out blood sampling；2) after blood sampling, sample mixing is carried out, has the suction pipe of blood sample to be inserted into mixing vessel acquisition,
Diaphragm seal is punctured by suction pipe, blood sample injection treatment fluid is housed layer to be mixed with treatment fluid.
Blood sample sampling pretreatment device provided by the present invention, by hollow suction pipe automatic sucking blood sample,
Encapsulation process liquid in sample mixing unit, has been reduced as far as the two and has been contacted with external environment, and blood sampling portion and sample are mixed
Conjunction portion is detachably connected the rapid abutting joint that can realize blood sampling and treatment fluid, when mixing, only need to suction pipe be punctured diaphragm seal,
It squeezes again in elastomer injection blood sample to mixed liquor, rocks for use after mixing vessel, needing only need to be by drain list when using
Member is opened, and mixed liquor can be discharged by squeezing elastomer again.The apparatus structure is simple, in fast and easy, mixed liquor manufacturing process
Almost completely cut off completely with external environment, reduces contaminated probability and biological safety is high.
Blood sample provided by the invention samples preprocess method, and being placed in progress blood in blood sample by suction pipe adopts
Acquisition is had the suction pipe of blood sample to be inserted into mixing vessel, punctures diaphragm seal by suction pipe, blood sample is injected treatment fluid by sample
Accommodating layer is mixed with treatment fluid, and whole process is isolated with external environment, and the mixed liquor produced will not be polluted by external environment
And biology is safe.
Description of the drawings
Fig. 1 is the structural schematic diagram of blood sample sampling pretreatment device of the present invention；
Fig. 2 is the structural schematic diagram of sample mixing unit in blood sample sampling pretreatment device of the present invention；
Fig. 3 is the enlarged drawing of compression opening and suction pipe junction in blood sample sampling pretreatment device of the present invention；
Fig. 4 is the flow frame diagram that blood sample of the present invention samples preprocess method.
Specific implementation mode
To facilitate the understanding of the structure of the invention, it illustrates with reference to the accompanying drawings and embodiments.
Fig. 1 is the structural schematic diagram of blood sample sampling pretreatment device of the present invention, and Fig. 2 samples for blood sample of the present invention
The structural schematic diagram of sample mixing unit in pretreatment unit, Fig. 3 are to compress to open in blood sample sampling pretreatment device of the present invention
The enlarged drawing of mouth and suction pipe junction.In conjunction with shown in Fig. 1 to Fig. 3, the present invention provides a kind of blood samples to sample pretreatment dress
It sets, including：The blood sampling portion 10 being detachably connected and sample mixing unit 20.
The blood sampling portion 10 includes a hollow suction pipe 11, and one end of the suction pipe 11 is communicated with elastomer 12, institute
The inside for stating elastomer 12 is enclosed with the compression stroke of the resiliently elastic wall of the elastomer, and the elastic wall is equipped with compression
Opening 121, the compression opening 121 and the junction of the suction pipe 11 are equipped with a sealing ring 41, and the two of the sealing ring 41
Side is equipped with the adhesive layer 42 for sealing the compression opening 121 and the junction of the suction pipe, which avoids suction pipe 11 and bullet
Property body 12 directly bond, three layers of closed protection are realized by the form of adhesive layer 42- sealing ring 41- adhesive layers 42, by blood
The inner space of sampling unit 10 completely cuts off completely with external environment.In the present embodiment, the front end of the suction pipe 11 is set as puncture tip shape.
The sample mixing unit 20 includes the mixing vessel 21 for accommodating suction pipe 11, the lower half in the mixing vessel 21
Portion is equipped with the diaphragm seal 211 punctured for the end of the suction pipe 11, the wall shape of the diaphragm seal 211 and the mixing vessel 21
At there is closed treatment fluid to house layer 212, the bottom of the mixing vessel 21, which is additionally provided with, to be housed layer 212 with the treatment fluid and is connected to
Drain unit 213, the top of the mixing vessel 21 is equipped with the vessel port of size corresponding with the caliber of the suction pipe 11
214, so that the elastomer 12 is fixed in the vessel port 214.
Baffle 215 is equipped in the mixing vessel 21 of vessel port 213, the baffle 215 is equipped with corresponding institute
The connecting hole 2151 of 11 caliber of suction pipe is stated, the baffle 215 and the wall of the mixing vessel 21 are formed with installation space 216,
Guiding piece 218 is equipped in the installation space 216,218 middle part of the guiding piece is equipped with the guiding groove of the corresponding suction pipe outer diameter
2181.While suction pipe 11 wears mixing vessel 21, to be guided to suction pipe by guiding groove 2181.
It is soft that 2151 peripheral wall of connecting hole of the baffle 215 is equipped with the elastic packaging being arranged towards 21 bottom direction of mixing vessel
Colloid 217, in the present embodiment, the elastic packaging flexible glue body 217 has a hollow cyclic annular flexible glue ontology 2171, and with it is described
The centrum interconnecting piece 2172 that flexible glue ontology is connected and volume is tapered.Further, the centrum interconnecting piece 2172 includes contradicting to set
The multiple arcs flap valve set, when not plugging suction pipe, multiple arcs flap valve is in closed state, mixed to prevent external contaminants matter from entering
It closes in container 21, after plugging suction pipe 11, multiple arcs flap valve is wrapped on the tube wall of suction pipe 11, can also prevent external contaminants
Matter enters in mixing vessel.
Further, the elastomer 12 and the junction of the suction pipe 11 are formed with the L-shaped card slot in cross section, described
Vessel port 214 is contradicted with the card slot and is connected.The setting enables elastomer 12 to be seated 214 top of vessel port, and L
After the card slot of type contradicts vessel port 214, further, inner space is completely cut off and has been contacted with external environment.
In addition, the sample mixing unit 20 further includes the protection cap 30 of sealing container opening, the protection cap 30, which has, to be received
Hold the guard space of the elastomer 12.In the present embodiment, the protection cap 30 has the first lid 31 of receiving elastomer, with
And be connected with first lid 31 and towards the second lid 32 that 21 bottom direction of the mixing vessel extends, described second
32 ring set of lid is on the side wall of mixing vessel 21, and second lid 32 is equipped with groove ring 321, the mixing vessel 21
321 position of respective slot ring is equipped with bulge loop 22.Further, first lid 31 has the first of corresponding vessel port 214
Lid mouth, the first lid mouth are equipped with the easy abscission ring that is collapsed towards middle part, the pipe of the elastomer 12 of the easy abscission ring and cylindrical shape
Wall corresponds to size setting, and the easy abscission ring is equipped with rubber finger sleeve, and the opening plaster of the rubber finger sleeve is together on the easy abscission ring.When
When 214 upper cover of vessel port closes protection cap 30, elastomer 12 passes through the hollow open of easy abscission ring, directly causes easy abscission ring by sealing container
Opening 214 is contradicted, and quickly breaks easy abscission ring, final latex set is wrapped in vessel port.The setting is further advanced by breast
Gum cover is sealed the connecting pin of elastomer 12 and vessel port 214, further reduces contaminated possibility.It need to lift
It is noted that there is drain unit 213 drain pipe set on 21 bottom of mixing vessel, the drain pipe to be equipped with and be detachably connected
Drain plug.
Fig. 4 is the flow frame diagram that blood sample of the present invention samples preprocess method, referring to Fig. 4, the present invention also provides
A kind of blood sample samples preprocess method, includes the following steps：
Suction pipe is placed in blood sample and carries out blood sampling by step S1, blood sampling.
Step S2 after blood sampling, carries out sample mixing, has the suction pipe of blood sample to be inserted into mixing vessel acquisition, leads to
It crosses suction pipe and punctures diaphragm seal, blood sample injection treatment fluid is housed layer to be mixed with treatment fluid.
The concrete application details of blood sample sampling pretreatment device provided by the invention is described in detail below.
First, the suction pipe 11 in blood sampling portion 10 is placed in blood sample, by siphon principle, blood sample is inhaled automatically
Enter in suction pipe 11, the suction pipe 11 for sucking blood sample is inserted into mixing vessel 21 by vessel port, is guided when suction pipe 11 enters
When slot 2181, the inner wall of guiding piece 218 is contacted with suction pipe 11, with guide suction pipe 11 quickly plant, suction pipe 11 is continued it is downward, directly
Diaphragm seal 211 is punctured to the front end of suction pipe 11, so that suction pipe 11 is inserted in treatment fluid and houses in layer 212, at this point, elastomer 12 is just
It is seated in the opening of sealing container 214.When need not temporarily mix blood sample and treatment fluid, directly protection cap 30 be located in
In vessel port 214, during this, elastomer 12 passes through the hollow open of easy abscission ring, and easy abscission ring is open institute by sealing container 214
It breaks, is wrapped in vessel port 214 to rubber finger sleeve, at this point, 32 ring set of the second lid is on the side wall of mixing vessel,
And second lid 32 is equipped with the engaging corresponding with the bulge loop of mixing vessel of groove ring.It needs to mix blood sample and treatment fluid
When, protection cap is taken out, elastomer 12 is then squeezed, blood sample is injected in treatment fluid, by rocking mixing vessel manually
21 so that mixed liquor is uniformly mixed, finally by the discharge mixed liquor of drain unit 213 of mixing vessel 21.
Blood sample sampling pretreatment device provided by the present invention, by hollow suction pipe automatic sucking blood sample,
Encapsulation process liquid in sample mixing unit, has been reduced as far as the two and has been contacted with external environment, and blood sampling portion and sample are mixed
Conjunction portion is detachably connected the rapid abutting joint that can realize blood sampling and treatment fluid, when mixing, only need to suction pipe be punctured diaphragm seal,
It squeezes again in elastomer injection blood sample to mixed liquor, rocks for use after mixing vessel, needing only need to be by drain list when using
Member is opened, and mixed liquor can be discharged by squeezing elastomer again.Using closing drain plug is finished, residual samples are made together with device
For offal treatment, influence of the sample to environment is effectively reduced.The apparatus structure is simple, fast and easy, mixed liquor manufacturing process
In almost completely cut off completely with external environment, it is high to reduce contaminated probability and biological safety.
Blood sample provided by the invention samples preprocess method, and being placed in progress blood in blood sample by suction pipe adopts
Acquisition is had the suction pipe of blood sample to be inserted into mixing vessel, punctures diaphragm seal by suction pipe, blood sample is injected treatment fluid by sample
Accommodating layer is mixed with treatment fluid, and whole process is isolated with external environment, and the mixed liquor produced will not be polluted by external environment
And biology is safe.
The present invention has been described in detail with reference to the accompanying drawings, those skilled in the art can be according to upper
It states and bright many variations example is made to the present invention.Thus, certain details in embodiment should not constitute limitation of the invention, this
Invention will be using the range that the appended claims define as protection domain.
Claims (10)
1. a kind of blood sample sampling pretreatment device, which is characterized in that including：The blood sampling portion being detachably connected and sample
Present mixing section；
The blood sampling portion includes a hollow suction pipe, and one end of the suction pipe is communicated with elastomer, the elastomer it is interior
Portion is enclosed with the compression stroke of the elastic wall of the resilient elastomer, and the elastic wall is equipped with compression and is open, the compression
The junction of opening and the suction pipe is equipped with a sealing ring, and the both sides of the sealing ring are equipped with and seal the compression opening and institute
State the adhesive layer of the junction of suction pipe；
The sample mixing unit includes the mixing vessel for accommodating suction pipe, and the lower half in the mixing vessel is equipped with described in confession
It is accommodating that the wall of the diaphragm seal that the end of suction pipe punctures, the diaphragm seal and the mixing vessel is formed with closed treatment fluid
Layer, the bottom of the mixing vessel is additionally provided with houses the drain unit that layer is connected to, the top of the mixing vessel with the treatment fluid
Portion is equipped with the vessel port of size corresponding with the caliber of the suction pipe, so that the elastomer is fixed in the vessel port.
2. blood sample sampling pretreatment device according to claim 1, which is characterized in that close to the described of vessel port
Baffle is equipped in mixing vessel, the baffle is equipped with the connecting hole of the corresponding suction pipe caliber, and the baffle is mixed with described
The wall of container is formed with installation space, and guiding piece is equipped in the installation space, is equipped in the middle part of the guiding piece described in corresponding to
The guiding groove of suction pipe outer diameter.
3. blood sample sampling pretreatment device according to claim 2, which is characterized in that the baffle connecting hole peripheral wall
It is equipped with the elastic packaging flexible glue body being arranged towards mixing vessel bottom direction.
4. blood sample sampling pretreatment device according to claim 3, which is characterized in that the elastic packaging flexible glue body
With hollow cyclic annular flexible glue ontology, and it is connected with the flexible glue ontology and centrum interconnecting piece that volume is tapered.
5. blood sample sampling pretreatment device according to claim 4, which is characterized in that the centrum interconnecting piece includes
Contradict the multiple arcs flap valve of setting.
6. blood sample sampling pretreatment device according to claim 5, which is characterized in that the elastomer and the suction
The junction of pipe is formed with the L-shaped card slot in cross section, and the vessel port is contradicted with the card slot and connected.
7. blood sample sampling pretreatment device according to claim 6, which is characterized in that the sample mixing unit is also wrapped
The protection cap of sealing container opening is included, the protection cap has the guard space for accommodating the elastomer.
8. blood sample sampling pretreatment device according to claim 7, which is characterized in that the protection cap has receiving
First lid of elastomer, and be connected with first lid and extend towards the mixing vessel bottom direction second
Lid, the second lid ring set is on the side wall of mixing vessel, and second lid is equipped with groove ring, and the mixing is held
Device respective slot ring position is equipped with bulge loop.
9. blood sample sampling pretreatment device according to claim 8, which is characterized in that first lid have pair
The first lid mouth of vessel port, the first lid mouth is answered to be equipped with the easy abscission ring collapsed towards middle part, the easy abscission ring and the suction
The tube wall of pipe corresponds to size setting, and the easy abscission ring is equipped with rubber finger sleeve, and the opening plaster of the rubber finger sleeve is together in the easy abscission ring
On.
It is pre-processed 10. being sampled according to the blood sample of blood sample sampling pretreatment device described in claim 1 to 9 any one
Method, which is characterized in that include the following steps：
1) suction pipe is placed in blood sample and carries out blood sampling by blood sampling；
2) after blood sampling, sample mixing is carried out, has the suction pipe of blood sample to be inserted into mixing vessel acquisition, is punctured by suction pipe
Blood sample injection treatment fluid is housed layer and is mixed with treatment fluid by diaphragm seal.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510867804.6A CN105510109B (en) | 2015-11-30 | 2015-11-30 | Blood sample sampling pretreatment device and its method |
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| CN201510867804.6A CN105510109B (en) | 2015-11-30 | 2015-11-30 | Blood sample sampling pretreatment device and its method |
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| CN105510109A CN105510109A (en) | 2016-04-20 |
| CN105510109B true CN105510109B (en) | 2018-08-10 |
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| CN201510867804.6A Active CN105510109B (en) | 2015-11-30 | 2015-11-30 | Blood sample sampling pretreatment device and its method |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10159973B2 (en) * | 2016-08-31 | 2018-12-25 | Toshiba Medical Systems Corporation | Sample liquid preparing apparatus, test kit, and sample liquid preparing method |
| CN107569240A (en) * | 2017-08-30 | 2018-01-12 | 台州市黄岩方野科技发展有限公司 | A kind of micro blood-taking tube of preset reagent and blood sampling and the method for adding reagent |
| CN110292961B (en) * | 2019-07-02 | 2021-07-20 | 英诺维尔智能科技（苏州）有限公司 | Sealing structure of pipette tip |
| CN111956272B (en) * | 2020-09-03 | 2023-03-24 | 河南省中医院（河南中医药大学第二附属医院） | Cervical cancer epithelial cell sampling device |
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| CN1495420A (en) * | 1998-03-06 | 2004-05-12 | �����ɡ���ά | Automatic resealed container cap |
| JP3709554B2 (en) * | 2000-11-20 | 2005-10-26 | 時男 桐山 | Liquid introduction device for liquid chromatography column |
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|---|---|---|---|---|
| JP3963563B2 (en) * | 1998-04-08 | 2007-08-22 | 株式会社アイ・デザイン | Blood collection tube |
| JP4158095B2 (en) * | 2003-01-15 | 2008-10-01 | 株式会社クニムネ | Urine sample collection and storage equipment |
| JP2010139433A (en) * | 2008-12-12 | 2010-06-24 | Olympus Corp | Blood collecting device and blood collecting method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1495420A (en) * | 1998-03-06 | 2004-05-12 | �����ɡ���ά | Automatic resealed container cap |
| JP3709554B2 (en) * | 2000-11-20 | 2005-10-26 | 時男 桐山 | Liquid introduction device for liquid chromatography column |
| CN203929425U (en) * | 2014-05-21 | 2014-11-05 | 艾博生物医药(杭州)有限公司 | A kind of sample collection bottle |
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| CN105510109A (en) | 2016-04-20 |
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Address after: 430074 No.18, East bio Garden Road, Donghu New Technology Development Zone, Wuhan City, Hubei Province Patentee after: Wuhan Jinghong Technology Co., Ltd Address before: 430074, building 7-5, 6 Industrial Park, Kanto New Technology Development Zone, Wuhan, Hubei, East Lake Patentee before: Wuhan Jinghong Wanfangtang Medical Technology Co., Ltd. |
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Denomination of invention: Blood sample sampling pretreatment device and method Effective date of registration: 20191220 Granted publication date: 20180810 Pledgee: Bank of Hankou Limited by Share Ltd Financial Services Center Pledgor: Wuhan Jinghong Technology Co., Ltd Registration number: Y2019980001183 |