CN105502665A - Garbage leachate wastewater biological comprehensive treatment method - Google Patents
Garbage leachate wastewater biological comprehensive treatment method Download PDFInfo
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- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
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Abstract
The invention discloses a garbage leachate wastewater biological comprehensive treatment method. Specifically, four sewage treatment pools are built firstly, a sand filter strip is disposed in each sewage treatment pool, aquatic plant reed is planted on the sand filter strips, the fourth sewage pool is a sand pool, in which aquatic plant reed is planted, eichhornia crassipes and Ludwigia peploides are planted in the first, second and third sewage pools to culture Egyptian fish (Clarias leather), aquatic plant reed is planted in the fourth sewage sand pool, and a biological preparation has to be added before planting waterweed and culturing fish. The garbage leachate wastewater biological comprehensive treatment method provided by the invention has the advantages of low cost, green and environmental protection, and the planted plants can create economic benefits, thus changing the passive situation that sewage treatment just requires investment but has no income.
Description
Technical field
The invention belongs to field of Environment Protection, particularly a kind of refuse leachate wastewater biological integrated conduct method
Background technology
Percolate is in rubbish hydrolysis and ferments or rain drop erosion, the sewage that the processes such as the immersion of surface water and groundwater filter out.Have tremendous influence and destruction because water quality is very complicated to surrounding environment, be that universally acknowledged pollution threat is large, character is complicated, be difficult to the high-concentration waste water that processes, the main refuse leachate treatment process of current China has:
1. physico-chemical processes; The multiple methods such as the film dialysis such as chemical precipitation method, active carbon adsorption, chemical oxidization method, reverse osmosis method, air lift and wet oxidation process.Advantage is that effluent quality is more stable, and treatment effect is better, and shortcoming is that processing cost is higher, is not suitable for processing a large amount of waste water;
2. electricity flocculation EC method; It is world today's latest generation electrochemical water treatment technology, utilize principle of electrochemical reaction, producing electrochemical reaction electric energy conversion by impressed voltage effect is chemical energy, organic or inorganic pollutent in waste water is oxidized and reduction reaction, and then it is cohesion, floating except pollutent is separated from water body, advantage is the various noxious pollutant such as COD, heavy metal, SS, oil, phosphoric acid salt can effectively removed in waste water, greatly improve the biodegradability of waste water, shortcoming is that constantly to drop into management expectancy high simultaneously;
3. biological process; (having aerobic or anaerobism, Aerobic-anaerobic integrated process)
Aerobe method makes good use of oxygen animalcule to remove BOD, COD in sewage and some heavy metal ion etc.Main method is activated sludge process, SBR method, CASS method etc.;
Anaerobic biological process mainly contains anaerobic contact method, anaerobic biofilter, upflow sludge bed reactor etc., advantage is that floor space is little, power consumption is few, but also can generate energy (methane gas), halogen organic can be solved, the penetrating fluid process lower for phosphorus content is more applicable, shortcoming be for temperature and pH value requirement high, and generally can not directly to discharge through the penetrating fluid of anaerobic treatment;
Aerobic-anaerobic integrated process be integrate aerobic-anaerobic advantage to the clearance of BOD, COD all more than 90%, treatment effect is good, and shortcoming disposablely has high input and constantly drop into;
4. membrane processing method; Comparatively common a kind of processing mode, relatively good to the water treatment effect not containing organic substance, selectivity percolate can be divided into two not identical parts: concentrated solution and clear water, and pollutent is trapped within concentration liquid.Membrane processing method all has good removal effect for COD, BOD, the TN etc. in percolate, effluent quality can reach miscellaneous water standard direct reuse, shortcoming is that the membrane lifetime that process penetrating fluid adopts is short, easy pollution, cost is higher, and process is not thorough, the concentrated solution that film process produces also needs further process;
5. MVC method of evaporation: the process that volatile matter is separated with non-volatile substance, it is made up of two portions: one is that heated solution makes water boiling vaporization, and two is water vapors that constantly removing is vaporized.While employing MVC evaporation technology, be equipped with other corresponding assisting workflows, advantage is that energy consumption is low, water quality is excellent, bookkeeping is conveniently stablized, keep in repair easy and cost is low, shortcoming disposablely has high input and constantly drop into;
In present field of Environment Protection, the process of percolate or highly energy-consuming high investment are without the primary stage of return, just can complete by national relevant policies and financial support.
Summary of the invention
The present invention be directed to the deficiencies in the prior art, a kind of refuse leachate wastewater biological integrated conduct method is provided.
The technical scheme that the present invention solves the problems of the technologies described above is as follows:
1. a refuse leachate wastewater biological integrated conduct method, first builds four treatment tanks, is husky transition band between each, planting aquatic plants reed on husky transition band, and the 4th treatment tank is Sha Chi, planting aquatic plants reed in husky pond, each treatment tank is built into trapezoidal, first treatment tank is higher than second treatment tank, 4th the husky pond of sewage disposal is minimum by that analogy, first treatment tank plants Herba Eichhorniae and the chrysanthemum fire hose in treatment tank to three sewage lagoon, cultivate Egyptian fish (Egyptian Clarias fuscus, have another name called clarias leather, Egyptian pool carp louse), must drop into Mace tower desulfurization bacterium DesulfobacteriummacestiiCGMCC1.3477 before planting pasture and water and breeding fish, Bacillus subtilis subspecies Bacillussubtilissubsp.spizizeniiCGMCC1.268, scarlet Nocardia bacteria NocardiarubropertinctaCGMCC4.103, slowly to take root knurl Bradyrhizobiumjaponicum, CGMCC1.909, actinobacillus capsulatus Actinobacilluscapsulatus, ACCC11051, Bacillus licheniformis BacillulicheniformisCGMCC1.521 and bacillus megaterium Bacillusmegaterium, CGMCC1.1870, it is main biotechnological formulation, in described biotechnological formulation, the bacterium number of each bacterial classification all>=5.0 × 10
8cfu/ml.
2 one kinds of refuse leachate wastewater biological integrated conduct methods, comprise the following steps:
1) treatment tank is built:
(1) four treatment tanks are first built, it is husky transition band between each, 4th sewage lagoon is Sha Chi, sewage lagoon is built into trapezoidal first treatment tank higher than second treatment tank, 4th the husky pond of sewage is minimum by that analogy, first treatment tank, second treatment tank, the size of the 3rd sewage lagoon is according to being: the day amount of disposing of sewage × 15 calculating, antiseep must be carried out in each pond, in case effluent seepage contaminate environment, the design of intake is in the bottom in pond, water outlet design is on the top in pond, the water-in of husky transition band is one end, water outlet is the other end, the length of husky transition band is equal with the length of sewage lagoon, width is 20 meters, the degree of depth is: day the amount of disposing of sewage × 3) 10 to calculate, pond is built into trapezoidal, first treatment tank higher than second treatment tank by that analogy, 4th the husky pond of sewage is minimum, dark 10 meters of 4th sewage Sha Chi pond, area is 2 times of first treatment tank, water-in is one end, water outlet is the other end.
(2) treatment tank plantation and cultivation:
First treatment tank, second treatment tank, 3rd sewage lagoon plantation Herba Eichhorniae and the chrysanthemum fire hose, cultivate Egyptian fish (Egyptian Clarias fuscus, have another name called clarias leather, Egyptian pool carp louse), planting aquatic plants reed on husky transition band, planting aquatic plants reed in the 4th the husky pond of sewage.
2) preparation of seed culture fluid:
(1) Bacillus subtilis subspecies Bacillussubtilissubsp.spizizeniiCGMCC1.268, actinobacillus capsulatus Actinobacilluscapsulatus, ACCC11051, Bacillus licheniformis BacillulicheniformisCGMCC1.521, bacillus megaterium Bacillusmegaterium, CGMCC1.1870, the preparation of seed culture fluid: be aseptically inoculated in respectively in liquid nutrient medium, described liquid nutrient medium is: the content of each component in 1.0L distilled water is: peptone 10.0g, extractum carnis 3.0g, sodium-chlor 5.0g, pH value is adjusted to 7.0, cultivate 48 hours under 28 DEG C ~ 32 DEG C conditions, bacterium number>=5.0 × 10 obtained
8the seed culture fluid of cfu/ml.
(2) preparation of Mace tower desulfurization bacterium DesulfobacteriummacestiiCGMCC1.3477 seed culture fluid: be inoculated under aseptic anaerobic condition in liquid nutrient medium, described liquid nutrient medium is:
Solution A: K2HPO40.5g, NH4Cl1.0g, Na2SO41.0g, CaCl22H2O0.1g, MgSO47H2O2.0g, DL-LACTIC ACID sodium 2.0g, yeast extract 1.0g, Resazurin1.0mg, distilled water 980.0ml;
Solution B: FeSO47H2O0.5g, distilled water 10.0ml;
Solution C: Thioglycolic acid sodium salt 0.1g, vitamins C 0.1g, distilled water 10.0ml;
Solution A is chilled to room temperature after boiling several minutes, adds anaerobic N2 gas.With the pH to 7.8 of NaOH regulator solution B and C, and packing under N2 (at any time wave and culture base) is in anaerobism pipe.Sterilizing 15 points under 121 DEG C of conditions.Anaerobic culturel is cultivated 48 hours under 32 DEG C ~ 45 DEG C conditions, bacterium number>=5.0 × 10 obtained
8the seed culture fluid of cfu/ml.
(3) to take root slowly knurl Bradyrhizobiumjaponicum, the preparation of CGMCC1.909 seed culture fluid: be aseptically inoculated in liquid nutrient medium, described liquid nutrient medium is: the content of each component in 1.0L distilled water is: KH2PO40.2g, K2HPO40.8g, MgSO47H2O0.2g, CaSO42H2O0.1g, FeCl3 trace g, Na2MoO42H2O trace g, yeast extract 0.5g, N.F,USP MANNITOL 20.0g, pH7.2, cultivate 48 hours under 28 DEG C ~ 32 DEG C conditions, bacterium number>=5.0 × 10 obtained
8the seed culture fluid of cfu/ml.
(4) CGMCC1.268, the preparation of scarlet Nocardia bacteria NocardiarubropertinctaCGMCC4.103 seed culture fluid: be aseptically inoculated in liquid nutrient medium, described liquid nutrient medium is: the content of each component in 1.0L distilled water is: glucose 10.0g; Asparagine 0.5g; K2HPO40.5g; Cultivate 48 hours for pH7.2-7.428 DEG C ~ 32 DEG C, bacterium number>=5.0 × 10 obtained
8the seed culture fluid of cfu/ml.
The weight percentage of the seed culture fluid of described each bacterial classification is: Mace tower desulfurization bacterium 10% ~ 30%, Bacillus subtilis subspecies 10% ~ 30%, scarlet Nocardia bacteria 10% ~ 30%, slowly to take root knurl 10% ~ 30%, actinobacillus capsulatus 10% ~ 30%, Bacillus licheniformis 10% ~ 30%, bacillus megaterium 10% ~ 30%.
Above-mentioned multi-cultur es microbial compound preparation preparation method, step is as follows:
(1) aseptically, by Mace tower desulfurization bacterium DesulfobacteriummacestiiCGMCC1.3477, Bacillus subtilis subspecies Bacillussubtilissubsp.spizizeniiCGMCC1.268, scarlet Nocardia bacteria NocardiarubropertinctaCGMCC4.103, slowly to take root knurl Bradyrhizobiumjaponicum, CGMCC1.909, actinobacillus capsulatus Actinobacilluscapsulatus, ACCC11051, Bacillus licheniformis BacillulicheniformisCGMCC1.521, bacillus megaterium Bacillusmegaterium, CGMCC1.1870, be linked into respectively in substratum, inoculum size is 5 ﹪ of substratum weight, air flow 0.5 ~ 1.0L/Lmin, stirring velocity 150 ~ 200rpm, tank pressure 0.5kg/cm
2, oxygen consumption cultivates 48 hours, bacterium number>=5.0 × 10 in the bacterium liquid obtained
8cfu/mL, Mace tower desulfurization bacterium DesulfobacteriummacestiiCGMCC1.3477 does not need ventilation and is stirred in normal pressure anaerobically fermenting.
Above-mentioned substratum percentage is: starch 5 ﹪, soybean cake powder 7.5 ﹪, dipotassium hydrogen phosphate 0.2 ﹪, ammonium sulfate 0.1 ﹪, magnesium sulfate 0.05 ﹪, iron trichloride 0.005 ﹪, calcium carbonate 0.05 ﹪, yeast powder 0.01 ﹪, boric acid 0.005 ﹪, pH value are adjusted to 7.2 ~ 7.4.
(2) composite
Each bacterium liquid step (1) prepared according to weight percentage is: Mace tower desulfurization bacterium 10% ~ 30%, Bacillus subtilis subspecies 10% ~ 30%, scarlet Nocardia bacteria 10% ~ 30%, slowly to take root knurl 10% ~ 30%, actinobacillus capsulatus 10% ~ 30%, Bacillus licheniformis 10% ~ 30%, bacillus megaterium 10% ~ 30%. aseptically mixes, and obtains multi-cultur es microbial compound preparation.
Bacterial classification function selected by the present invention is as follows:
(1) Mace tower desulfurization bacterium DesulfobacteriummacestiiCGMCC1.3477, the biological thiobacillus (Thiobacillus) mostly of chemosynthetic autotroph, can oxidation of sulfureted hydrogen, elementary sulfur, thiosulphate and tetrathionate etc., and form sulfuric acid, and from then on obtain energy in process.
(2) Bacillus subtilis subspecies Bacillussubtilissubsp.spizizeniiCGMCC1.268 has very strong restraining effect to harmful microorganisms such as the vibrios in aquatic products, intestinal bacteria and baculoviruss, the function of a large amount of chitinase can be secreted, the cell walls of chitinase decomposable asymmetric choice net pathogenic fungi and Antifungi disease, decompose the hazardous and noxious substances in culturing pool, purify water; There is stronger proteolytic enzyme, lipase, diastatic activity, ight soil, organism etc. in decomposing pool, there is the short grained effect of rubbish in very strong cleaning water; Can improve water turbidity problem, water quality is clear by muddy change, has very strong Function For Purifying Water.
(3) scarlet Nocardia bacteria NocardiarubropertinctaCGMCC4.103, this genus bacterium is distributed widely in nature, and it is the producing strains of Multiple Classes of Antibiotics.In addition, also play a role in oil dewaxing, sewage purification.
(4) take root slowly knurl Bradyrhizobiumjaponicum, CGMCC1.909 is aerobic, take oxygen as the strict breathing pattern of terminal electron acceptor.Chemoheterotrophic bacteria, utilize various carbohydrate and organic acid as carbon source, ammonium salt, nitrate and some amino acids can be used as nitrogenous source, so the ammonia nitrogen that can consume in sewage purifies water, can fix atmospheric nitrogen to bonding state nitrogen (ammonia) by host plant is utilized.Some bacterial strains are fixed my waterplant of atmospheric nitrogen with unbound state under given conditions and are provided nutrition.
5. actinobacillus capsulatus Actinobacilluscapsulatus, ACCC11051, the production of antibiotics of application, produces various zymin (proteolytic enzyme, amylase and cellulase etc.), VITAMIN (B12) and organic acid etc.Also can be used for the aspects such as Steroid Transformation, hydro carbons fermentation, oil dewaxing and sewage disposal.Many organism can be decomposed, comprise the compound of aromatic compound, paraffin, rubber, Mierocrystalline cellulose, the strong toxicity such as complex compound and some cyanogen such as wooden.Therefore, actinomycetes, not only in nature material cycle, more have positive effect in the biological treatment of sewage and organic solid waste.
6. Bacillus licheniformis BacillulicheniformisCGMCC1.521, there is abundant proteolytic enzyme, lipase, amylase, cellulase etc., it can intensive decomposition carbon system, nitrogen system, phosphorus system, sulphur system pollutent, decompose complicated polysaccharide, protein and water soluble organic substance, form dominant microflora in water surrounding.
7. bacillus megaterium Bacillusmegaterium, CGMCC1.1870, very strong restraining effect is had to harmful microorganisms such as the vibrios in aquatic products, intestinal bacteria and baculoviruss, it has the function of secreting a large amount of chitinase, the cell walls of chitinase decomposable asymmetric choice net pathogenic fungi and Antifungi disease, and there is stronger proteolytic enzyme, lipase, diastatic activity, can promote that the organism in decomposition sewage lagoon and hazardous and noxious substances purify water, there is very strong Function For Purifying Water;
Beneficial effect of the present invention is as follows:
1. the present invention is without the need to investing various filter plant.
2. the present invention is without the need to investing sewage treatment plant.
3. employment of the present invention is few, and cost of labor is low.
4. the Herba Eichhorniae in the present invention can be used for fertilizer, and reed is economical as there being certain income.
5. sewage work of the present invention beautiful environment, can go sightseeing to play.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail, but the present invention is not only confined to following examples.
In following embodiment, described bacterial classification is provided by Chinese agriculture Microbiological Culture Collection administrative center and China General Microbiological culture presevation administrative center.
Embodiment 1
A kind of refuse leachate wastewater biological integrated conduct method, first building four treatment tanks, is husky transition band between each, planting aquatic plants reed on husky transition band, and the 4th treatment tank is Sha Chi, planting aquatic plants reed in husky pond, each treatment tank is built into trapezoidal, first treatment tank is higher than second treatment tank, 4th the husky pond of sewage disposal is minimum by that analogy, first treatment tank plants Herba Eichhorniae and the chrysanthemum fire hose in treatment tank to three sewage lagoon, cultivate Egyptian fish (Egyptian Clarias fuscus, have another name called clarias leather, Egyptian pool carp louse), must drop into Mace tower desulfurization bacterium DesulfobacteriummacestiiCGMCC1.3477 before planting pasture and water and breeding fish, Bacillus subtilis subspecies Bacillussubtilissubsp.spizizeniiCGMCC1.268, scarlet Nocardia bacteria NocardiarubropertinctaCGMCC4.103, slowly to take root knurl Bradyrhizobiumjaponicum, CGMCC1.909, actinobacillus capsulatus Actinobacilluscapsulatus, ACCC11051, Bacillus licheniformis BacillulicheniformisCGMCC1.521 and bacillus megaterium Bacillusmegaterium, CGMCC1.1870, it is main biotechnological formulation, in described biotechnological formulation, the bacterium number of each bacterial classification all>=5.0 × 10
8cfu/ml.
Embodiment 2
A kind of refuse leachate wastewater biological integrated conduct method, step is as follows:
1) treatment tank is built:
(1) four treatment tanks are first built, it is husky transition band between each, 4th sewage lagoon is Sha Chi, sewage lagoon is built into trapezoidal first treatment tank higher than second treatment tank, 4th the husky pond of sewage is minimum by that analogy, first treatment tank, second treatment tank, the size of the 3rd sewage lagoon is according to being: the day amount of disposing of sewage × 15 calculating, antiseep must be carried out in each pond, in case effluent seepage contaminate environment, the design of intake is in the bottom in pond, water outlet design is on the top in pond, the water-in of husky transition band is one end, water outlet is the other end, the length of husky transition band is equal with the length of sewage lagoon, width is 20 meters, the degree of depth is: day the amount of disposing of sewage × 3) 10 to calculate, pond is built into trapezoidal, first treatment tank higher than second treatment tank by that analogy, 4th the husky pond of sewage is minimum, dark 10 meters of 4th sewage Sha Chi pond, area is 2 times of first treatment tank, water-in is one end, water outlet is the other end.
(2) treatment tank plantation and cultivation:
First treatment tank, second treatment tank, 3rd sewage lagoon plantation Herba Eichhorniae and the chrysanthemum fire hose, cultivate Egyptian fish (Egyptian Clarias fuscus, have another name called clarias leather, Egyptian pool carp louse), planting aquatic plants reed on husky transition band, planting aquatic plants reed in the 4th the husky pond of sewage.
2) preparation of seed culture fluid:
(1) Bacillus subtilis subspecies Bacillussubtilissubsp.spizizeniiCGMCC1.268, actinobacillus capsulatus Actinobacilluscapsulatus, ACCC11051, Bacillus licheniformis BacillulicheniformisCGMCC1.521, bacillus megaterium Bacillusmegaterium, CGMCC1.1870, the preparation of seed culture fluid: be aseptically inoculated in respectively in liquid nutrient medium, described liquid nutrient medium is: the content of each component in 1.0L distilled water is: peptone 10.0g, extractum carnis 3.0g, sodium-chlor 5.0g, pH value is adjusted to 7.0, cultivate 48 hours under 28 DEG C of conditions, bacterium number>=5.0 × 10 obtained
8the seed culture fluid of cfu/ml.
(2) preparation of Mace tower desulfurization bacterium DesulfobacteriummacestiiCGMCC1.3477 seed culture fluid: be inoculated under aseptic anaerobic condition in liquid nutrient medium, described liquid nutrient medium is:
Solution A: K
2hPO
40.5g, NH
4cl1.0g, Na
2sO
41.0g, CaCl
22H
2o0.1g, MgSO
47H
2o2.0g, DL-LACTIC ACID sodium 2.0g, yeast extract 1.0g, Resazurin1.0mg, distilled water 980.0ml;
Solution B: FeSO
47H
2o0.5g, distilled water 10.0ml;
Solution C: Thioglycolic acid sodium salt 0.1g, vitamins C 0.1g, distilled water 10.0ml;
Solution A is chilled to room temperature after boiling several minutes, adds anaerobic N
2gas.With the pH to 7.8 of NaOH regulator solution B and C, and packing under N2 (at any time wave and culture base) is in anaerobism pipe.Sterilizing 15 points under 121 DEG C of conditions.Anaerobic culturel is cultivated 48 hours under 32 DEG C of conditions, bacterium number>=5.0 × 10 obtained
8the seed culture fluid of cfu/ml.
(3) to take root slowly the preparation of knurl Bradyrhizobiumjaponicum, CGMCC1.909 seed culture fluid:
Aseptically be inoculated in liquid nutrient medium, described liquid nutrient medium is: the content of each component in 1.0L distilled water is: KH
2pO
40.2g, K
2hPO
40.8g, MgSO
47H
2o0.2g, CaSO
42H
2o0.1g, FeCl
3trace g, Na
2moO
42H
2o trace g, yeast extract 0.5g, N.F,USP MANNITOL 20.0g, pH7.2, cultivate 48 hours, bacterium number>=5.0 × 10 obtained under 28 DEG C of conditions
8the seed culture fluid of cfu/ml.
(4) CGMCC1.268, the preparation of scarlet Nocardia bacteria NocardiarubropertinctaCGMCC4.103 seed culture fluid: be aseptically inoculated in liquid nutrient medium, described liquid nutrient medium is: the content of each component in 1.0L distilled water is: glucose 10.0g; Asparagine 0.5g; K2HPO40.5g; Cultivate 48 hours for pH7.2-7.428 DEG C, bacterium number>=5.0 × 10 obtained
8the seed culture fluid of cfu/ml.
The weight percentage of the seed culture fluid of described each bacterial classification is: Mace tower desulfurization bacterium 10%, Bacillus subtilis subspecies 10%, scarlet Nocardia bacteria 10%, slowly to take root knurl 10%, actinobacillus capsulatus 10%, Bacillus licheniformis 10%, bacillus megaterium 10%.
Above-mentioned multi-cultur es microbial compound preparation preparation method, step is as follows:
(1) aseptically, by Mace tower desulfurization bacterium DesulfobacteriummacestiiCGMCC1.3477, Bacillus subtilis subspecies Bacillussubtilissubsp.spizizeniiCGMCC1.268, scarlet Nocardia bacteria NocardiarubropertinctaCGMCC4.103, slowly to take root knurl Bradyrhizobiumjaponicum, CGMCC1.909, actinobacillus capsulatus Actinobacilluscapsulatus, ACCC11051, Bacillus licheniformis BacillulicheniformisCGMCC1.521, bacillus megaterium Bacillusmegaterium, CGMCC1.1870, be linked into respectively in substratum, inoculum size is 5 ﹪ of substratum weight, air flow 0.5L/Lmin, stirring velocity 150rpm, tank pressure 0.5kg/cm
2, oxygen consumption cultivates 48 hours, bacterium number>=5.0 × 10 in the bacterium liquid obtained
8cfu/mL, Mace tower desulfurization bacterium DesulfobacteriummacestiiCGMCC1.3477 does not need ventilation and is stirred in normal pressure anaerobically fermenting.
Above-mentioned substratum percentage is: starch 5 ﹪, soybean cake powder 7.5 ﹪, dipotassium hydrogen phosphate 0.2 ﹪, ammonium sulfate 0.1 ﹪, magnesium sulfate 0.05 ﹪, iron trichloride 0.005 ﹪, calcium carbonate 0.05 ﹪, yeast powder 0.01 ﹪, boric acid 0.005 ﹪, pH value are adjusted to 7.2 ~ 7.4.
(2) composite
Each bacterium liquid step (1) prepared according to weight percentage is: Mace tower desulfurization bacterium 10%, Bacillus subtilis subspecies 10%, scarlet Nocardia bacteria 10%, slowly to take root knurl 10%, actinobacillus capsulatus 10%, Bacillus licheniformis 10%, bacillus megaterium 10%. aseptically mixes, and obtains multi-cultur es microbial compound preparation.
Embodiment 2
A kind of refuse leachate wastewater biological integrated conduct method, step is as follows:
1) treatment tank is built:
With the step 1 of embodiment 1) identical.
2) preparation of seed culture fluid:
(1) Bacillus subtilis subspecies Bacillussubtilissubsp.spizizeniiCGMCC1.268, actinobacillus capsulatus Actinobacilluscapsulatus, ACCC11051, Bacillus licheniformis BacillulicheniformisCGMCC1.521, bacillus megaterium Bacillusmegaterium, CGMCC1.1870, the preparation of seed culture fluid: be aseptically inoculated in respectively in liquid nutrient medium, described liquid nutrient medium is: the content of each component in 1.0L distilled water is: peptone 10.0g, extractum carnis 3.0g, sodium-chlor 5.0g, pH value is adjusted to 7.0, cultivate 48 hours under 32 DEG C of conditions, bacterium number>=5.0 × 10 obtained
8the seed culture fluid of cfu/ml.
(2) preparation of Mace tower desulfurization bacterium DesulfobacteriummacestiiCGMCC1.3477 seed culture fluid: be inoculated under aseptic anaerobic condition in liquid nutrient medium, described liquid nutrient medium is:
Solution A: K
2hPO
40.5g, NH
4cl1.0g, Na
2sO
41.0g, CaCl
22H
2o0.1g, MgSO
47H
2o2.0g, DL-LACTIC ACID sodium 2.0g, yeast extract 1.0g, Resazurin1.0mg, distilled water 980.0ml;
Solution B: FeSO47H2O0.5g, distilled water 10.0ml;
Solution C: Thioglycolic acid sodium salt 0.1g, vitamins C 0.1g, distilled water 10.0ml;
Solution A is chilled to room temperature after boiling several minutes, adds anaerobic N2 gas.With the pH to 7.8 of NaOH regulator solution B and C, and packing under N2 (at any time wave and culture base) is in anaerobism pipe.Sterilizing 15 points under 121 DEG C of conditions.Anaerobic culturel is cultivated 48 hours under 45 DEG C of conditions, bacterium number>=5.0 × 10 obtained
8the seed culture fluid of cfu/ml.
(3) to take root slowly the preparation of knurl Bradyrhizobiumjaponicum, CGMCC1.909 seed culture fluid: be aseptically inoculated in liquid nutrient medium, described liquid nutrient medium is: the content of each component in 1.0L distilled water is: KH
2pO
40.2g, K
2hPO
40.8g, MgSO
47H
2o0.2g, CaSO
42H
2o0.1g, FeCl
3trace g, Na
2moO
42H
2o trace g, yeast extract 0.5g, N.F,USP MANNITOL 20.0g, pH7.2, cultivate 48 hours, bacterium number>=5.0 × 10 obtained under 32 DEG C of conditions
8the seed culture fluid of cfu/ml.
(4) CGMCC1.268, the preparation of scarlet Nocardia bacteria NocardiarubropertinctaCGMCC4.103 seed culture fluid: be aseptically inoculated in liquid nutrient medium, described liquid nutrient medium is: the content of each component in 1.0L distilled water is: glucose 10.0g; Asparagine 0.5g; K
2hPO
40.5g; Cultivate 48 hours for pH7.2-7.432 DEG C, bacterium number>=5.0 × 10 obtained
8the seed culture fluid of cfu/ml.
The weight percentage of the seed culture fluid of described each bacterial classification is: Mace tower desulfurization bacterium 30%, Bacillus subtilis subspecies 30%, scarlet Nocardia bacteria 30%, slowly to take root knurl 30%, actinobacillus capsulatus 30%, Bacillus licheniformis 30%, bacillus megaterium 30%.
Above-mentioned multi-cultur es microbial compound preparation preparation method, step is as follows:
(1) aseptically, by Mace tower desulfurization bacterium DesulfobacteriummacestiiCGMCC1.3477, Bacillus subtilis subspecies Bacillussubtilissubsp.spizizeniiCGMCC1.268, scarlet Nocardia bacteria NocardiarubropertinctaCGMCC4.103, slowly to take root knurl Bradyrhizobiumjaponicum, CGMCC1.909, actinobacillus capsulatus Actinobacilluscapsulatus, ACCC11051, Bacillus licheniformis BacillulicheniformisCGMCC1.521, bacillus megaterium Bacillusmegaterium, CGMCC1.1870, be linked into respectively in substratum, inoculum size is 5 ﹪ of substratum weight, air flow 0.5 ~ 1.0L/Lmin, stirring velocity 200rpm, tank pressure 0.5kg/cm
2, oxygen consumption cultivates 48 hours, bacterium number>=5.0 × 10 in the bacterium liquid obtained
8cfu/mL, Mace tower desulfurization bacterium DesulfobacteriummacestiiCGMCC1.3477 does not need ventilation and is stirred in normal pressure anaerobically fermenting.
Above-mentioned substratum percentage is: starch 5 ﹪, soybean cake powder 7.5 ﹪, dipotassium hydrogen phosphate 0.2 ﹪, ammonium sulfate 0.1 ﹪, magnesium sulfate 0.05 ﹪, iron trichloride 0.005 ﹪, calcium carbonate 0.05 ﹪, yeast powder 0.01 ﹪, boric acid 0.005 ﹪, pH value are adjusted to 7.2 ~ 7.4.
(2) composite
Each bacterium liquid step (1) prepared according to weight percentage is: Mace tower desulfurization bacterium 30%, Bacillus subtilis subspecies 30%, scarlet Nocardia bacteria 30%, slowly to take root knurl 30%, actinobacillus capsulatus 30%, Bacillus licheniformis 30%, bacillus megaterium 30%. aseptically mixes, and obtains multi-cultur es microbial compound preparation.
Claims (3)
1. a refuse leachate wastewater biological integrated conduct method, it is characterized in that: first build four treatment tanks, is husky transition band between each, planting aquatic plants reed on husky transition band, 4th treatment tank is Sha Chi, planting aquatic plants reed in husky pond, each treatment tank is built into trapezoidal, first treatment tank is higher than second treatment tank, 4th husky pond is minimum by that analogy, first treatment tank plants Herba Eichhorniae and the chrysanthemum fire hose in treatment tank to three sewage lagoon, cultivate Egyptian fish (Egyptian Clarias fuscus, have another name called clarias leather, Egyptian pool carp louse), must drop into Mace tower desulfurization bacterium DesulfobacteriummacestiiCGMCC1.3477 before planting pasture and water and breeding fish, Bacillus subtilis subspecies Bacillussubtilissubsp.spizizeniiCGMCC1.268, scarlet Nocardia bacteria NocardiarubropertinctaCGMCC4.103, slowly to take root knurl Bradyrhizobiumjaponicum, CGMCC1.909, actinobacillus capsulatus Actinobacilluscapsulatus, ACCC11051, Bacillus licheniformis BacillulicheniformisCGMCC1.521 and bacillus megaterium Bacillusmegaterium, CGMCC1.1870, biotechnological formulation, in described biotechnological formulation, the bacterium number of each bacterial classification all>=5.0 × 10
8cfu/ml.
2. a kind of refuse leachate wastewater biological integrated conduct method as claimed in claim 1, is characterized in that, comprise the following steps:
1) treatment tank is built:
(1) four treatment tanks are first built, it is husky transition band between each, 4th sewage lagoon is Sha Chi, sewage lagoon is built into trapezoidal first treatment tank higher than second treatment tank, 4th the husky pond of sewage is minimum by that analogy, first treatment tank, second treatment tank, the size of the 3rd sewage lagoon is according to being: the day amount of disposing of sewage × 15 calculating, antiseep must be carried out in each pond, in case effluent seepage contaminate environment, the design of intake is in the bottom in pond, water outlet design is on the top in pond, the water-in of husky transition band is one end, water outlet is the other end, the length of husky transition band is equal with the length of sewage lagoon, width is 20 meters, the degree of depth is: day the amount of disposing of sewage × 3 10 to calculate, pond is built into trapezoidal, first treatment tank higher than second treatment tank by that analogy, 4th the husky pond of sewage is minimum, dark 10 meters of 4th sewage Sha Chi pond, area is 2 times of first treatment tank, water-in is one end, water outlet is the other end,
(2) treatment tank plantation and cultivation:
First treatment tank, second treatment tank, 3rd sewage lagoon plantation Herba Eichhorniae and the chrysanthemum fire hose, cultivate Egyptian fish (Egyptian Clarias fuscus, have another name called clarias leather, Egyptian pool carp louse), planting aquatic plants reed on husky transition band, planting aquatic plants reed in the 4th the husky pond of sewage.
2) preparation of seed culture fluid:
(1) Bacillus subtilis subspecies Bacillussubtilissubsp.spizizeniiCGMCC1.268, actinobacillus capsulatus Actinobacilluscapsulatus, ACCC11051, Bacillus licheniformis BacillulicheniformisCGMCC1.521, bacillus megaterium Bacillusmegaterium, CGMCC1.1870, the preparation of seed culture fluid: be aseptically inoculated in respectively in liquid nutrient medium, described liquid nutrient medium is: the content of each component in 1.0L distilled water is: peptone 10.0g, extractum carnis 3.0g, sodium-chlor 5.0g, pH value is adjusted to 7.0, cultivate 48 hours under 28 DEG C ~ 32 DEG C conditions, bacterium number>=5.0 × 10 obtained
8the seed culture fluid of cfu/ml,
(2) preparation of Mace tower desulfurization bacterium DesulfobacteriummacestiiCGMCC1.3477 seed culture fluid: be inoculated under aseptic anaerobic condition in liquid nutrient medium, described liquid nutrient medium is:
Solution A: K2HPO40.5g, NH4Cl1.0g, Na2SO41.0g, CaCl22H2O0.1g, MgSO47H2O2.0g, DL-LACTIC ACID sodium 2.0g, yeast extract 1.0g, Resazurin1.0mg, distilled water 980.0ml;
Solution B: FeSO47H2O0.5g, distilled water 10.0ml;
Solution C: Thioglycolic acid sodium salt 0.1g, vitamins C 0.1g, distilled water 10.0ml;
Solution A is chilled to room temperature after boiling several minutes, adds anaerobic N2 gas; With the pH to 7.8 of NaOH regulator solution B and C, and point be filled in anaerobism pipe under N2, sterilizing 15 points under 121 DEG C of conditions; Anaerobic culturel is cultivated 48 hours under 32 DEG C ~ 45 DEG C conditions, bacterium number>=5.0 × 10 obtained
8the seed culture fluid of cfu/ml;
(3) to take root slowly knurl Bradyrhizobiumjaponicum, the preparation of CGMCC1.909 seed culture fluid: be aseptically inoculated in liquid nutrient medium, described liquid nutrient medium is: the content of each component in 1.0L distilled water is: KH2PO40.2g; K2HPO40.8g; MgSO47H2O0.2g; CaSO42H2O0.1g; FeCl3 trace g; Na2MoO42H2O trace g; Yeast extract 0.5g; N.F,USP MANNITOL 20.0g; PH7.2, cultivates 48 hours for 28 DEG C ~ 32 DEG C, bacterium number>=5.0 × 10 obtained
8the seed culture fluid of cfu/ml;
(4) CGMCC1.268, the preparation of scarlet Nocardia bacteria NocardiarubropertinctaCGMCC4.103 seed culture fluid: be aseptically inoculated in liquid nutrient medium, described liquid nutrient medium is: the content of each component in 1.0L distilled water is: glucose 10.0g; Asparagine 0.5g; K2HPO40.5g; Cultivate 48 hours for pH7.2-7.428 DEG C ~ 32 DEG C, bacterium number>=5.0 × 10 obtained
8the seed culture fluid of cfu/ml;
The weight percentage of the seed culture fluid of described each bacterial classification is: Mace tower desulfurization bacterium 10% ~ 30%, Bacillus subtilis subspecies 10% ~ 30%, scarlet Nocardia bacteria 10% ~ 30%, slowly to take root knurl 10% ~ 30%, actinobacillus capsulatus 10% ~ 30%, Bacillus licheniformis 10% ~ 30%, bacillus megaterium 10% ~ 30%.
3. a kind of refuse leachate wastewater biological integrated conduct method according to claim 2, is characterized in that, described multi-cultur es microbial compound preparation preparation method, and step is as follows:
(1) aseptically, by Mace tower desulfurization bacterium DesulfobacteriummacestiiCGMCC1.3477, Bacillus subtilis subspecies Bacillussubtilissubsp.spizizeniiCGMCC1.268, scarlet Nocardia bacteria NocardiarubropertinctaCGMCC4.103, slowly to take root knurl Bradyrhizobiumjaponicum, CGMCC1.909, actinobacillus capsulatus Actinobacilluscapsulatus, ACCC11051, Bacillus licheniformis BacillulicheniformisCGMCC1.521, bacillus megaterium Bacillusmegaterium, CGMCC1.1870, be linked into respectively in substratum, inoculum size is 5 ﹪ of substratum weight, air flow 0.5 ~ 1.0L/Lmin, stirring velocity 150 ~ 200rpm, tank pressure 0.5kg/cm
2, oxygen consumption cultivates 48 hours, bacterium number>=5.0 × 10 in the bacterium liquid obtained
8cfu/mL, Mace tower desulfurization bacterium DesulfobacteriummacestiiCGMCC1.3477 does not need ventilation and is stirred in normal pressure anaerobically fermenting,
Substratum percentage described above is: starch 5 ﹪, soybean cake powder 7.5 ﹪, dipotassium hydrogen phosphate 0.2 ﹪, ammonium sulfate 0.1 ﹪, magnesium sulfate 0.05 ﹪, iron trichloride 0.005 ﹪, calcium carbonate 0.05 ﹪, yeast powder 0.01 ﹪, boric acid 0.005 ﹪, pH value are adjusted to 7.2 ~ 7.4;
(2) composite
Each bacterium liquid step (1) prepared according to weight percentage is: Mace tower desulfurization bacterium 10% ~ 30%, Bacillus subtilis subspecies 10% ~ 30%, scarlet Nocardia bacteria 10% ~ 30%, slowly to take root knurl 10% ~ 30%, actinobacillus capsulatus 10% ~ 30%, Bacillus licheniformis 10% ~ 30%, bacillus megaterium 10% ~ 30%. aseptically mixes, and obtains multi-cultur es microbial compound preparation.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6043022A (en) * | 1996-05-21 | 2000-03-28 | Board Of Control Of Michigan Technological University | Apparatus and method for the generation and use of ferric ions |
| CN101428927A (en) * | 2008-08-04 | 2009-05-13 | 上海环境工程技术有限公司 | Combined process for treatment and qualified discharge of garbage leachate |
| CN101434907A (en) * | 2008-12-18 | 2009-05-20 | 天津大学 | Microbial preparation for processing refuse leachate and preparation |
| CN102391966A (en) * | 2011-11-17 | 2012-03-28 | 苏柯汉（潍坊）生物工程有限公司 | Composite bacteria for treating food waste percolate and preparation method thereof |
| CN104560857A (en) * | 2015-01-26 | 2015-04-29 | 鹭滨环保科技（上海）有限公司 | Ruffondia bacillus brevis and construction method thereof and application of ruffondia bacillus brevis in treatment of high-concentration ammonia-nitrogen wastewater |
-
2015
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6043022A (en) * | 1996-05-21 | 2000-03-28 | Board Of Control Of Michigan Technological University | Apparatus and method for the generation and use of ferric ions |
| CN101428927A (en) * | 2008-08-04 | 2009-05-13 | 上海环境工程技术有限公司 | Combined process for treatment and qualified discharge of garbage leachate |
| CN101434907A (en) * | 2008-12-18 | 2009-05-20 | 天津大学 | Microbial preparation for processing refuse leachate and preparation |
| CN102391966A (en) * | 2011-11-17 | 2012-03-28 | 苏柯汉（潍坊）生物工程有限公司 | Composite bacteria for treating food waste percolate and preparation method thereof |
| CN104560857A (en) * | 2015-01-26 | 2015-04-29 | 鹭滨环保科技（上海）有限公司 | Ruffondia bacillus brevis and construction method thereof and application of ruffondia bacillus brevis in treatment of high-concentration ammonia-nitrogen wastewater |
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