CN105494931A - Method for extracting amino acid from stems and leaves of peanut - Google Patents
Method for extracting amino acid from stems and leaves of peanut Download PDFInfo
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- CN105494931A CN105494931A CN201510878861.4A CN201510878861A CN105494931A CN 105494931 A CN105494931 A CN 105494931A CN 201510878861 A CN201510878861 A CN 201510878861A CN 105494931 A CN105494931 A CN 105494931A
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- amino acid
- peanut
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- leaves
- acid powder
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Abstract
The invention discloses a method for extracting amino acid from stems and leaves of peanut. The method comprises the following steps: adding water to stems and leaves of peanut for three times of hot-water extraction and controlling the temperature, wherein the amount of the added water is 5-7 times of that of the stems and leaves of peanut each time; merging extracting solutions obtained in three times; filtering and concentrating; controlling the volume and the feed ratio of the stems and leaves of peanut; adding ethanol; controlling the concentration of ethanol; separating the sediment; concentrating the supernatant; when no ethanol exists, performing column chromatography separation to obtain amino acid powder. The method aims to extract free amino acid from the raw materials (the stems and the leaves of peanut), the effective protein value of the amino acid powder extracted by the method is high, the production process is simple, the operation is easy, the cost is low, and the extracted amino acid powder is an efficient, nontoxic and safe feed fortifying additive and can be directly used for feeding addition of high-quality meat livestock.
Description
Technical field
The invention belongs to amino acid preparing technical field, be specifically related to one from peanut stem leaf, extract amino acid whose method.
Background technology
Green blade has become protein source maximum in the world.Utilize fresh plant stem-leaf to extract leaf protein, supplement human food and animal feed albumen as dietary protein origin, caused the attention of countries in the world.China's plant resources abundant species is the treasure-house extracting plant leaf protein.
Along with the change of human diet structure, pursue commodity matter, trend that low-fat meat, fish, eggs have become aquaculture development, the fodder strengthening agent of various high protein, high-energy, high effective element arises at the historic moment, aquaculture is also transitioned into scientific, standardization raising from home cage, wherein the most directly, the most rapidly can supplement all kinds of amino acid whose shortage in penkeeping process with amino acid feed hardening agent, without hydrolysis directly by gastrointestinal absorption, protein for high nutrition synthesizes, and plays the most completely, nutritive effect the most thoroughly.Therefore, the hardening agent of various products of amino acid has also entered into feed market gradually.
Summary of the invention
The object of the invention is from peanut stem leaf raw material, to extract in order to providing a kind of the method extracting amino acid powder from peanut stem leaf that effective protein score, PS is high, production process is simple, easy to operate, cost is low.
Object of the present invention realizes by following measure:
A kind of method extracting amino acid powder from peanut stem leaf contains following step: first peanut stem leaf is added hydro-thermal and carry 3 times, each heating 2-4 hour, and temperature is 80-90 DEG C, and each amount of water is 5-7 times of peanut stem leaf; Merge No. three extracts, filtrations, concentrate volumes to when being l:l with peanut stem leaf rate of charge, add ethanol again, controlling concentration of alcohol in solution is 60-70%, sediment separate out after leaving standstill, concentrated supernatant to during without ethanol content, then obtains amino acid powder through column chromatography for separation.
Object of the present invention also realizes by following measure:
Described extract from peanut stem leaf in the method for amino acid powder adopt column chromatography for separation mode namely to adopt ion exchange resin and after resin again through active carbon, aluminium oxide, macroreticular resin class chromatographic material, the i.e. separable amino acid powder obtaining the content range of 10-40%.
The present invention compared to existing technology tool has the following advantages:
1. natural peanut stem leaf flooding is removed impurity with alcohol settling by the present invention, recycle different column chromatography for separation and obtain the amino acid powder of Available Amino Acids content from the different stage of 10-40%: thus production process of the present invention is simple, easy to operate, abundant raw material source, with low cost.
2. the total amount of the free amino acid in raw material of the present invention is 2-3%, possesses 18 seed amino acids of protein synthesis, and collocation rationally, and wherein eight kinds of necessary amino acid whose content are higher; Utilizing effective protein score, PS of the product of this plant production to reach 35% ± 2%, is a kind of efficient, nontoxic, and the feed enhancement additive of safety, the nursing that can directly act on high-quality meat livestock is added.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described in further detail, can implement according to this with reference to description word to make those skilled in the art.
Experimental technique described in following embodiment, if no special instructions, is conventional method, described reagent and material, if no special instructions, all can obtain from commercial channels.
Embodiment
Get the peanut stem leaf dried in the shade and be cut into a cun section, put in extractor, add 6 times to the water of raw material, heating extraction 3 times, each 3 hours, it was 85 DEG C that temperature controls, merge 3 aqueous extracts, when to be concentrated into volume and raw material ratio be 1:1, add industrial alcohol, the concentration of alcohol controlling solution is 63%, filter after leaving standstill, the ethanol purge of filter residue 60-65% once, merges cleaner liquid, reclaims in ethanol to concentrate and can't smell ethanol taste.Above-mentioned concentrate is added in processed good strongly acidic cation-exchange post, first wash the non-absorbed portion of removing ion exchange resin with water, use the ammoniacal liquor wash-out of 0.5N again, collect and ninhydrin is positive part, be concentrated into dry, pulverize 120 mesh sieves, obtain the amino acid feed hardening agent that amino acid content is 15-20%.
Claims (2)
1. from peanut stem leaf, extract a method for amino acid powder, it is characterized in that the method contains following step: first peanut stem leaf is added hydro-thermal and carry 3 times, each heating 2-4 hour, temperature is 80-90 DEG C, and each amount of water is 5-7 times of peanut stem leaf; Merge No. three extracts, filter, concentrate volume to when being 1:1 with peanut stem leaf rate of charge, then add ethanol, controlling concentration of alcohol in solution is 60-70%, sediment separate out after leaving standstill, and when concentrated supernatant is extremely without ethanol, then obtains amino acid powder through column chromatography for separation.
2. from peanut stem leaf, extract the method for amino acid powder as claimed in claim 1, it is characterized in that the method adopt column chromatography for separation mode namely to adopt ion exchange resin and after resin again through active carbon, aluminium oxide, macroreticular resin class chromatographic material, the i.e. separable amino acid powder obtaining the content range of 10-40%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510878861.4A CN105494931A (en) | 2015-12-04 | 2015-12-04 | Method for extracting amino acid from stems and leaves of peanut |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510878861.4A CN105494931A (en) | 2015-12-04 | 2015-12-04 | Method for extracting amino acid from stems and leaves of peanut |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105494931A true CN105494931A (en) | 2016-04-20 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510878861.4A Withdrawn CN105494931A (en) | 2015-12-04 | 2015-12-04 | Method for extracting amino acid from stems and leaves of peanut |
Country Status (1)
| Country | Link |
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| CN (1) | CN105494931A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108186429A (en) * | 2017-10-20 | 2018-06-22 | 新疆于田瑰觅生物科技股份有限公司 | A kind of method that amino acid is prepared from rose slag |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1232021A (en) * | 1998-04-14 | 1999-10-20 | 中国科学院西北高原生物研究所 | Process for extracting all amino acid powder from plant |
| CN103642862A (en) * | 2013-12-02 | 2014-03-19 | 济南开发区星火科学技术研究院 | Method for separating and extracting composite amino acid from hibiscus syriacus leaf |
| CN103652307A (en) * | 2013-12-03 | 2014-03-26 | 山西医科大学 | Method for extracting leaf proteins from broussonetia papyrifera leaf dry powder and comprehensive utilization of waste |
-
2015
- 2015-12-04 CN CN201510878861.4A patent/CN105494931A/en not_active Withdrawn
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1232021A (en) * | 1998-04-14 | 1999-10-20 | 中国科学院西北高原生物研究所 | Process for extracting all amino acid powder from plant |
| CN103642862A (en) * | 2013-12-02 | 2014-03-19 | 济南开发区星火科学技术研究院 | Method for separating and extracting composite amino acid from hibiscus syriacus leaf |
| CN103652307A (en) * | 2013-12-03 | 2014-03-26 | 山西医科大学 | Method for extracting leaf proteins from broussonetia papyrifera leaf dry powder and comprehensive utilization of waste |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108186429A (en) * | 2017-10-20 | 2018-06-22 | 新疆于田瑰觅生物科技股份有限公司 | A kind of method that amino acid is prepared from rose slag |
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Application publication date: 20160420 |
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| WW01 | Invention patent application withdrawn after publication |