CN105482953A - Production process of selenium-rich blueberry healthcare wine - Google Patents
Production process of selenium-rich blueberry healthcare wine Download PDFInfo
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Abstract
The invention belongs to the technical field of healthcare wine processing, and particularly relates to a production process of selenium-rich blueberry healthcare wine. The production process of the selenium-rich blueberry healthcare wine comprises the following steps that 1 culture activation is carried out; 2 fermentation liquor is prepared from brewing yeast in the step 1; 3 fermentation is carried out, wherein the fermentation liquor in the step 2 is inoculated with rich selenium; 4 filtering is carried out, wherein the brewing yeast is separated; 5 blueberries and Chinese herbal medicine are pretreated, a fermentation medium is obtained, the yeast in the step 4 is inoculated with the culture medium for fermentation, ultrasonic wall breaking, filtering, ageing, blending, membrane filtration and sterile filling are carried out, and the selenium-rich blueberry healthcare wine is obtained. The blueberries are adopted as a formulation basis, multiple kinds of Chinese herbal medicine with the effects of resisting oxidation and aging is used for compatibility, the content of organic selenium in the prepared healthcare wine is high, the special Chinese herbal medicine healthcare wine is rich in nutrition, convenient to eat and unique in flavor, and the needs of people for healthy healthcare food are met.
Description
Technical field
The invention belongs to health promoting wine processing technique field, be specifically related to a kind of production technique of rich selenium blueberry mixed health wine.
Background technology
Selenium maintains one of biogenic element of human normal, and scarce selenium can cause the generation of various diseases.In whole food, selenium content is general lower, the normal need of people can not be met near the selenium in natural food, the low-level of Food Selenium is taken in has potential threat to HUMAN HEALTH, ensure HUMAN HEALTH, prevent and treat diseases related, sufficient selenium just should be had to take in, seeming of diseases related is prevented to be even more important by absorbing enough selenium, and inorganic selenium toxicity higher can not utilize by the mankind, by plant and microorganism, inorganic selenium is changed into the focus that organoselenium is research at present, but the selenium content in plant materials is general lower, thus by organic selenium product that microbial transformation can provide high yield inexpensive for the mankind.The focus that selenium-enriched food exploitation has become in health food development.
In recent years, along with the raising day by day of standard of living, the health perception of people is more and more stronger, consumption and the purchasing power of health-product market also constantly increase, particularly herbal health care product contains extremely abundant nutritive ingredient, and not only wherein amino acid classes is complete, is rich in the various trace elements such as potassium, calcium, and VITAMIN, the dark favor by human consumer.Fermented type health promoting wine is not only containing the active nutrient component in vegetable Chinese herbal medicine, human body being had to health-care effect, and produce a large amount of various nutritive ingredients (as sugar, protein, essential amino acid etc.) during the fermentation, appropriateness is drunk and can be played good health-care effect, becomes the product of herbal health care field of food and even whole field of health care food most development prospect.
Confirm through scientific research at present, containing abundant nutritive and health protection components in Blueberry, especially the content of anthocyanidin is far above other vegetables and fruit.Often edible blue berry goods, can significantly vision enhancing, anti-eye strain, skin nutrition, delay cranial nerve aging, have therapeutic action, cardiac function enhancing, prevention senile dementia etc. to the disease of capillaries caused by diabetes.The function of American-European scientific research personnel to blue berry conducted extensive research in recent years, have developed much blue berry food, healthcare products and medicine.
CN103602553B discloses a kind of Compound fermentation blueberry health-care wine and production method thereof, blueberry, Blue berry leaf are selected in this invention, matrimony vine, wheat bran be raw material by complex ferment production technique production Composite fermentation type health promoting wine, in wherein said composite fermentation liquid, each component proportion is: Blue berry leaf extracting solution 17 ~ 23%, blueberry juice 6 ~ 10%, wolfberry juice 10 ~ 15%, wheat bran saccharified liquid 55 ~ 65%.This product is of high nutritive value, natural nutrient component in basic maintenance blueberry, Blue berry leaf, and be rich in the nutritive substance such as anthocyanidin, VITAMIN of needed by human body, there is vision enhancing, anti-eye strain, skin nutrition, moistening lung, strengthening spleen and nourishing stomach, delay cranial nerve aging, prevent the effects such as disease of capillaries, cardiac function enhancing, prevention senile dementia, antifatigue.Advantage of the present invention is: one is the technique adopting ultrasonic-assisted extraction, and by fermentable effect, active substance extraction yield is high; Two is adopt Blue berry leaf, blueberry, matrimony vine and wheat bran complex ferment, improves the trophic level of blueberry mixed health wine; Three is the technique adopting micro-pore-film filtration, and product clarity is high, degerming, good impurity removing effect, instead of traditional pasteurization, and product Middle nutrition component damages is little.
Summary of the invention
In order to solve above-mentioned technical problem, the invention provides a kind of is main raw material with blueberry, be aided with plurality of Chinese component as matrimony vine, Radix Et Caulis Acanthopanacis Senticosi, Ligusticum wallichii, Herba Epimedii, the bighead atractylodes rhizome etc., synergy, jointly play anti-oxidant, anti-ageing, to improve immunity of organisms effect, and health promoting wine of the present invention adopts the two stage fermentation zymotechnique of high reactivity yeast rich in selenium inorganic selenium to be converted into security and the better organoselenium of nutritional type, improves the organic selenium content in health promoting wine.
The production technique of rich selenium blueberry mixed health wine of the present invention, comprises following step:
(1) actication of culture: activate rich selenium yeast saccharomyces cerevisiae;
(2) fermented liquid preparation: preparation yeast saccharomyces cerevisiae one-step fermentation substratum, obtains fermented liquid;
(3) ferment: the rich selenium yeast saccharomyces cerevisiae after activation in step (1) is inoculated in the fermented liquid of step (2);
(4) filter after fermentation ends, be separated yeast saccharomyces cerevisiae;
(5) mixing solutions is prepared;
Each raw material is prepared by following portions by weight:
Blueberry 40 ~ 50 parts, matrimony vine 20 ~ 30 parts, Radix Et Caulis Acanthopanacis Senticosi 15 ~ 20 parts, Ligusticum wallichii 12 ~ 15 parts, Herba Epimedii 8 ~ 10 parts, the bighead atractylodes rhizome 8 ~ 10 parts, 6 ~ 8 parts, the dried rhizome of rehmannia, the stem of noble dendrobium 4 ~ 6 parts, polygala root 3 ~ 5 parts, Tuber Fleeceflower Root 5 ~ 10 parts, Root of Kirilow Rhodiola 15 ~ 20 parts, 5 ~ 10 parts, Radix Glycyrrhizae, sweet Stevia 10 ~ 15 parts;
Blueberry pre-treatment: get blueberry, adds water, and soaks, and making beating, obtains blueberry pulp;
Herbal medicine pre-treatment: get herbal component, mixing after pulverizing, add pure water, then add cellulase, lixiviate under ultrasonic assistant condition, obtains herbal liquid medicine;
(6) mixing obtaining blueberry pulp in step (5) with Chinese herbal medicine liquid phase, in the mixing solutions obtained, adding inorganic salts, obtain herbal medicine fermention medium;
(7) yeast saccharomyces cerevisiae after step (4) being filtered is inoculated into herbal medicine fermention medium by the inoculum size of 10%;
(8) to the substratum fermentation obtained in step (7), supersonic wave wall breaking, filtration, ageing, allotment, membrane filtration, sterile filling, rich selenium blueberry mixed health wine is obtained.
Preferably, in above-mentioned step (1), rich for the high reactivity of preservation selenium yeast saccharomyces cerevisiae is activated 20-40min in the water of 30 ~ 40 DEG C.
Preferably, in above-mentioned step (2), the fermented liquid substratum of the yeast saccharomyces cerevisiae in preparation steps (1), is specially, glucose concn 3-6%, yeast extract paste concentration 0.6-1.5% in substratum, sterilizing 25-35min at 115-126 DEG C.
In above-mentioned step (3), rich for the high reactivity after activation selenium yeast saccharomyces cerevisiae is inoculated into fermented liquid, inoculative proportion 1.5-2.5%, under 28 ~ 32 DEG C of conditions, cultivates 48h, when cultivating 12h, 24h, 36h respectively to substratum in add the Sodium Selenite of 8-12g/L.
In above-mentioned step (5), blueberry pretreatment technology is: take blueberry, adds water, and the add-on of water is 2-5 times of blueberry weight, puts into 65-80 DEG C of water-bath and soaks 2 ~ 3h, then pull an oar;
The pretreatment technology of other herbal medicine above-mentioned is: get other above-mentioned herbal component, pulverized 40 mesh sieves, mixing, obtain herbal medicine medicinal powder, add water, the add-on of water is 6-10 times of Chinese herbal medicine grain weight amount, the water added is the pure water after adjusting pH7.5 ~ 8.0, then add cellulase, the add-on of cellulase is 1 ~ 2% of Chinese herbal medicine grain weight amount, lixiviate under ultrasonic assistant condition.
Preferred, in above-mentioned step (5), blueberry pretreatment technology is: take blueberry, adds water, and the add-on of water is 3 times of blueberry weight, puts into 75 DEG C of water-baths and soaks 2.5h, and making beating, obtains blueberry pulp;
The pretreatment technology of other herbal medicine above-mentioned is: get other above-mentioned herbal component, pulverized 40 mesh sieves, mixing, obtain herbal medicine medicinal powder, add water, the add-on of water is 6-10 times of Chinese herbal medicine grain weight amount, water is the pure water after adjusting pH7.8, then add cellulase, the add-on of cellulase is 1.5% of Chinese herbal medicine grain weight amount, and under ultrasonic assistant condition, lixiviate obtains herbal liquid medicine;
The condition of above-mentioned ultrasonic-assisted extraction is: ultrasonication 3 times, and each supersound process frequency is 20-100Hz, each sonication treatment time 2-8min, interval time 10-40s.
In above-mentioned step (6), inorganic salts and add-on thereof are specially: (NH
4)
2sO
41.5g/L, K
2hPO
41.5g/L and MgSO
41g/L, above " L " is often liter of mixing solutions.
In above-mentioned step (8), fermentation step specifically, temperature controlled fermentation under slow stirring, leavening temperature controls at 26 ~ 28 DEG C, and fermentation time is 36 ~ 48h;
Adopt ultrasonic wave mode to yeast broken wall 20 ~ 30min, more than mixing speed 300r/min during broken wall after fermentation ends;
Filtering solids insolubles, obtains wine liquid;
Wine liquid to be placed under the condition of 0 ~ 10 DEG C ageing 3 ~ 5 days;
Wine liquid after adjustment ageing makes its alcohol by volume concentration be 10 ~ 12%, then adds Xylitol, potassium sorbate, Tripotassium Citrate, oligomeric isomaltose, honey in wine liquid;
Each raw material is added by following weight proportion: 1 ~ 1.5g Xylitol, 3 ~ 5g potassium sorbate, 1 ~ 2g Tripotassium Citrate, 5 ~ 10g oligomeric isomaltose, 5 ~ 10g honey in often liter of wine liquid;
Membrane filtration: micro-pore-film filtration blueberry mixed health wine;
Sterile filling: filling and sealing obtains rich selenium blueberry mixed health wine in sterile filling production line.
Preferably, the production technique of rich selenium blueberry mixed health wine of the present invention, comprises following step:
(1) actication of culture: rich for the high reactivity of preservation selenium yeast saccharomyces cerevisiae is activated 30min in 37 DEG C of warm water;
(2) prepare fermented liquid: preparation yeast saccharomyces cerevisiae one-step fermentation substratum, in substratum, glucose concn 5%, yeast extract paste concentration 1%, 121 DEG C of sterilizing 30min, obtain fermented liquid;
(3) ferment: rich for high reactivity after activation in step (1) selenium yeast saccharomyces cerevisiae is inoculated in the fermented liquid of step (2), inoculative proportion is 2%, under 30 DEG C of conditions, cultivate 48h, when cultivating 12h, 24h, 36h respectively to substratum in add the Sodium Selenite of 10g/L;
(4) filter after fermentation ends, be separated yeast saccharomyces cerevisiae;
(5) mixing solutions is prepared;
Each raw material is prepared by following portions by weight:
Blueberry 45 parts, matrimony vine 25 parts, Radix Et Caulis Acanthopanacis Senticosi 18 parts, Ligusticum wallichii 14 parts, Herba Epimedii 9 parts, the bighead atractylodes rhizome 9 parts, 7 parts, the dried rhizome of rehmannia, the stem of noble dendrobium 5 parts, polygala root 4 parts, Tuber Fleeceflower Root 7 parts, Root of Kirilow Rhodiola 18 parts, 8 parts, Radix Glycyrrhizae, sweet Stevia 12 parts;
Blueberry pretreatment technology is: take blueberry, adds water, and the add-on of water is 3 times of blueberry weight, puts into 75 DEG C of water-baths and soaks 2.5h, and making beating, obtains blueberry pulp;
The pretreatment technology of herbal medicine is: get herbal component, pulverized 40 mesh sieves, mixing, obtain herbal medicine medicinal powder, add water, the add-on of water is 6-10 times of Chinese herbal medicine grain weight amount, water is the pure water after adjusting pH7.8, then add cellulase, the add-on of cellulase is 1.5% of Chinese herbal medicine grain weight amount, and under ultrasonic assistant condition, lixiviate obtains herbal liquid medicine;
The condition of above-mentioned ultrasonic-assisted extraction is: ultrasonication 3 times, and each supersound process frequency is 60Hz, each sonication treatment time 3min, interval time 10s, extraction temperature is 65 DEG C;
(6) obtain blueberry pulp and herbal medicine liquid-phase mixing by step (5), in the mixing solutions obtained, add inorganic salts, obtain herbal medicine fermention medium;
(7) yeast saccharomyces cerevisiae after filtration in step (4) is pressed the inoculum size inoculation herbal medicine fermention medium of 10%;
(8) fermentation, supersonic wave wall breaking, filtration, ageing, allotment, membrane filtration, sterile filling, obtains rich selenium blueberry mixed health wine;
Concrete steps are as follows: temperature controlled fermentation under slow stirring, and leavening temperature controls at 26 ~ 28 DEG C, and fermentation time is 36 ~ 48h;
Adopt ultrasonic wave mode to yeast broken wall 20 ~ 30min, more than mixing speed 300r/min during broken wall after fermentation ends;
Filtering solids insolubles, obtains wine liquid;
Wine liquid to be placed under 5 DEG C of conditions ageing 4 days;
Wine liquid after adjustment ageing makes its alcohol by volume concentration 11%, then adds Xylitol, potassium sorbate, Tripotassium Citrate, oligomeric isomaltose, honey in wine liquid;
Each raw material is added in following ratio: 1.2g Xylitol, 4g potassium sorbate, 1.5g Tripotassium Citrate, 8g oligomeric isomaltose, 8g honey in often liter of wine liquid;
Membrane filtration: the micro-pore-film filtration blueberry mixed health wine with aperture being 0.15 μm;
Sterile filling: filling and sealing obtains rich selenium blueberry mixed health wine in sterile filling production line.
Inorganic selenium is converted into organoselenium by yeast rich in selenium by rich selenium blueberry mixed health wine of the present invention, and inorganic selenium exists with oxidation state, by enteron aisle Passive intake, be reduced to selenide, then be transported to liver, the soluble form of synthesis Sethotope-selenium, or be transported to kidney and discharged by urine.In organoselenium, the valence state of selenium is normally with Se
2-valency exists, or is combined into complex state with other materials.The basic form of organoselenium is Sethotope, selenium cystine etc., exists, and is combined with amino acid, absorb with active form with reduction-state.The toxicity of organoselenium to animal is starkly lower than inorganic selenium, and is greater than inorganic selenium from the reserved of the natural organic selenium in meals in animal tissues.The experimental result of man and animal shows, the biopotency of the selenium in yeast rich in selenium is 10 ~ 20 times of inorganic selenium, and this just means that yeast rich in selenium is more suitable for the concentration improving selenium in animal food, and this will produce actively impact to the health care of the mankind.Rich selenium blueberry mixed health wine, based on anti-oxidant, the antidotal characteristic of organoselenium, is equipped with anti-oxidant, antidotal herbal medicine, thus exploitation has much the organoselenium blueberry mixed health wine of distinct Chinese characteristics.
The present invention take blueberry as prescription basis, compatibility is multiple have anti-oxidant, the herbal medicine of senile-resistant efficacy, the rich selenium fermentation by saccharomyces cerevisiae of high reactivity is utilized to have anti-oxidant, anti-ageing, improve the herbal medicine of the various health care functions such as immunity of organisms, prepare a kind of rich selenium blueberry mixed health wine newly, both blueberry was remained, the medical health care effect of the herbal medicine such as matrimony vine, expand the application approach of Chinese herbal health care wine, turn improve the organic selenium content in health promoting wine, a kind of nutritious, instant edible, the characteristic Chinese herbal health care wine of unique flavor, be applicable to multiple drunk by people, meet the demand of people to health-care food.
The invention provides a kind of rich selenium blueberry mixed health wine and production technique thereof, wherein the product of rich selenium blueberry mixed health wine has antifatigue, delays senility, improves the effects such as immunity of organisms, vision enhancing, anti-eye strain, skin nutrition, moistening lung, strengthening spleen and nourishing stomach, prevention disease of capillaries, cardiac function enhancing, prevention senile dementia; Transformation Application of the present invention not only can change drinks consumption result, meet the people to demand that is green, safe healthcare food, be of value to national health, and high-valuedly can make full use of Chinese herbal medicine resource, increase blueberry plant production development, increase the income of the blueberry growing area people, drive farmer richness.
The invention provides a kind of rich selenium blueberry mixed health wine and production technique thereof, it is advantageous that:
One is adopt the two stage fermentation zymotechnique of high reactivity yeast rich in selenium inorganic selenium to be converted into security and the better organoselenium of nutritional type, improve the organic selenium content in health promoting wine, in health promoting wine, the content of organoselenium reaches 300 ~ 350 μ g/L after testing, achieves unforeseeable technique effect;
Two is scientific compatibility blueberry and multiple herbal medicine, by the synergy between blueberry and herbal medicine, there is good antifatigue, delay senility, improve the effects such as immunity of organisms, vision enhancing, anti-eye strain, skin nutrition, moistening lung, strengthening spleen and nourishing stomach, prevention disease of capillaries, cardiac function enhancing, prevention senile dementia, safety has no side effect, instant.Different herbal medicine nature and flavor are different, effect also exists huge difference, complicated interaction relationship is there is after different herbal medicine combinations, and proportioning between herbal medicine is closer, different ratio also will make monarch relation that substantial change occurs, thus make the relation generation material alterations of composite Chinese herbal.
Complicated interaction relationship is there is to each other in the application by herbal medicine such as blueberry and matrimony vine, Radix Et Caulis Acanthopanacis Senticosi, Ligusticum wallichii, Herba Epimedii, the bighead atractylodes rhizome, the dried rhizome of rehmannia, the stem of noble dendrobium, polygala root, Tuber Fleeceflower Root, Root of Kirilow Rhodiola, Radix Glycyrrhizae, sweet Stevia, rational proportion, achieve unforeseeable technique effect, make the relation generation material alterations of rich selenium blueberry mixed health wine, product total antioxidant capacity reaches 721.14 ± 52.17U/mL, has good immunoregulatory activity; Adopt the indexs such as RNA isolation kit determination experiment mice skin tissue superoxide-dismutase (SOD), Selenoperoxidase (GSH-Px), mda (MDA) and oxyproline (Hyp), result shows that the indexs such as SOD, GSH-Px, Hyp are significantly higher than blank group (P<0.01), MDA, significantly lower than blank group (P<0.01), illustrates that product has the activity that delays senility preferably; Feed change composition or proportioning can reduce resistance of oxidation and the anti-ageing ability (P<0.01) of rich selenium blueberry mixed health wine;
Three is fermentation by high-activity yeast and the combined action of subsequent ultrasonic ripple broken wall, and significantly improve the extraction yield of saponin in herbal medicine, flavones, alkaloid isoreactivity material, detected result shows that saponin, flavones, alkaloid extraction rate reached are to more than 95%;
Four is the production technique adopting micro-pore-film filtration, and product clarity is high, degerming, good impurity removing effect, and sterilising effect is thorough, and instead of traditional pasteurization, product Middle nutrition component damages is little.
Embodiment
Below in conjunction with specific embodiment, the present invention is further described, so that those skilled in the art more understands the present invention, but does not therefore limit the present invention.
Embodiment 1
A production technique for rich selenium blueberry mixed health wine, it is characterized in that preparing by following method:
(1) actication of culture: rich for the high reactivity of preservation selenium yeast saccharomyces cerevisiae is activated 30min in the warm water of about 37 DEG C;
(2) fermented liquid preparation: preparation yeast saccharomyces cerevisiae one-step fermentation substratum, substratum glucose concn 5%, yeast extract paste concentration 1%, 121 DEG C of sterilizing 30min, obtain fermented liquid;
(3) ferment: be inoculated in the fermented liquid of step (2) by rich for high reactivity after activation selenium yeast saccharomyces cerevisiae, inoculative proportion 2%, cultivates 48h under the condition of 30 DEG C, when cultivating 12h, 24h, 36h respectively to substratum in add the Sodium Selenite of 10g/L;
(4) filter: filter after fermentation ends, be separated yeast saccharomyces cerevisiae;
(5) raw material is prepared by following portions by weight: blueberry 50 parts, matrimony vine 20 parts, Radix Et Caulis Acanthopanacis Senticosi 20 parts, Ligusticum wallichii 12 parts, Herba Epimedii 10 parts, the bighead atractylodes rhizome 8 parts, 8 parts, the dried rhizome of rehmannia, the stem of noble dendrobium 4 parts, polygala root 5 parts, Tuber Fleeceflower Root 5 parts, Root of Kirilow Rhodiola 20 parts, 5 parts, Radix Glycyrrhizae, sweet Stevia 15 parts;
(6) raw materials pretreatment:
Each raw material is taken by the parts by weight in step (5);
In above-mentioned raw materials, blueberry pretreatment technology is: the blueberry taking 50 parts, adds the water of 3 times of blueberry weight, is placed in 75 DEG C of water-baths and soaks 2.5h, then pull an oar;
In above-mentioned raw materials, the pretreatment technology of traditional Chinese medicinal components is: mix after raw material pulverizing, and crosses 40 mesh sieves, and obtain herbal medicine medicinal powder, add pure water, the add-on of water is about 8 times of herbal medicine medicinal powder, then adopts Na
2cO
3regulate the pH of pure water to be about 7.8, then add cellulase, add that weight accounts for herbal medicine weight 1.5% of cellulase, lixiviate under ultrasonic assistant condition, ultrasonication 6 times, each supersound process frequency is 60Hz, each sonication treatment time 3min, interval time 20s;
(7) herbal medicine fermention medium preparation: mix obtaining blueberry pulp in step (5) with pretreated Chinese herbal medicine fluid, inorganic salts is added in the mixing solutions obtained, obtain herbal medicine fermention medium, the Specific amounts that in often liter of mixing solutions, inorganic salt add is: 1.5g/L (NH
4)
2sO
4, 1.5g/LK
2hPO
4, 1g/LMgSO
4;
(8) inoculate: the yeast saccharomyces cerevisiae after step (4) being filtered inoculates herbal medicine fermention medium by the inoculum size of 10%;
Temperature controlled fermentation: temperature controlled fermentation under slow stirring, leavening temperature controls at about 27 DEG C, and fermentation time is about 40h;
Supersonic wave wall breaking: adopt ultrasonic wave mode to yeast broken wall 25min after fermentation ends, mixing speed about 400r/min during broken wall;
Filter: cross after fermentation ends and filter the solid insoluble such as Chinese herb residues and fermentation thalli;
Ageing: for promoting the stable and clarification just proposed, to be placed under the condition of about 5 DEG C ageing 4 days by wine liquid;
Allotment: the alcohol by volume concentration about 11% adjusting rich selenium blueberry mixed health wine, adds 1.5g/L Xylitol, 3g/L potassium sorbate, 2g/L Tripotassium Citrate, 5g/L oligomeric isomaltose, 10g/L honey;
Membrane filtration: the micro-pore-film filtration blueberry mixed health wine with aperture being 0.15 μm;
Sterile filling: filling and sealing obtains rich selenium blueberry mixed health wine in sterile filling production line.
Sensory evaluation scores:
Mark from following three aspects: local flavor 40 points, delicate fragrance alcohol and, aroma is strong, and all tastes is coordinated, and aroma is comparatively coordinated;
Mouthfeel 40 points, entrance is sweet, slightly tasty and refreshing bitter taste, and aftertaste is freely happy;
Structural state 20 points: as clear as crystal, uniformity, no suspended substance and throw out.
Through 50 expert analysis mode, the health promoting wine average in embodiment 1 is as follows:
Local flavor 39.85 points, mouthfeel 38.88 parts, structural state 18.58 points, adds up to score: 97.31 points.
In above-mentioned health promoting wine, the content of organoselenium is 330 μ g/L.
Resistance of oxidation is 764.14U/mL; (adopt total antioxidant capacity detection kit detection method, namely all adopt this method in (ABTS method) following examples, if no special instructions.
Select 100 women, wherein that 30-40 year is 30 people, 40-50 year be 50 people, 50-60 year be 20 people, above women has subhealth state problem in various degree, the bad black eye that cause of such as sleep quality, or the colour of skin is obscure; Or often easily suffer from the disease such as Common Cold, tonsillitis;
100 above women take health promoting wine 20mL of the present invention every night, and after one month, observe the colour of skin of these women, wherein 98 have the sign significantly having blast, and 90 sleeps take a favorable turn, and black eye alleviate;
Flu or inflammatory patients number are 0;
After two months, observe this 100 women, wherein 99 obvious blasts of skin, and also have 20 female skins to become and compact, 95 sleep states are good, and black eye alleviate;
Flu or inflammatory patients number are only 1 people.
Above-mentioned experiment shows, health promoting wine of the present invention has good antioxygenation, and the colour of skin for women highlights useful, but also improves immunity of organisms.
Embodiment 2
The production technique of rich selenium blueberry mixed health wine, its concrete steps are as follows:
(1) actication of culture: rich for the high reactivity of preservation selenium yeast saccharomyces cerevisiae is activated 30min in about 37 DEG C warm water;
(2) fermented liquid preparation: preparation yeast saccharomyces cerevisiae one-step fermentation substratum, substratum glucose concn 5%, yeast extract paste concentration 1%, 121 DEG C of sterilizing 30min, obtain fermented liquid;
(3) one-step fermentation: be inoculated in the fermented liquid of step (2) by rich for high reactivity after activation selenium yeast saccharomyces cerevisiae, inoculative proportion 2%, cultivates 48h under 30 DEG C of conditions, when cultivating 12h, 24h, 36h respectively to substratum in add the Sodium Selenite of 10g/L;
(4) filter: filter after fermentation ends, be separated yeast saccharomyces cerevisiae;
(5) raw material blueberry 40 parts, matrimony vine 30 parts, Radix Et Caulis Acanthopanacis Senticosi 15 parts, Ligusticum wallichii 15 parts, Herba Epimedii 8 parts, the bighead atractylodes rhizome 10 parts, 6 parts, the dried rhizome of rehmannia, the stem of noble dendrobium 6 parts, polygala root 3 parts, Tuber Fleeceflower Root 10 parts, Root of Kirilow Rhodiola 15 parts, 10 parts, Radix Glycyrrhizae, sweet Stevia 10 parts of ratio compatibilities are prepared in following portions by weight;
(6) raw materials pretreatment:
Each raw material is taken by the parts by weight in step (5):
Stating blueberry pretreatment technology in raw material is: the blueberry taking 50 parts, adds the water of 3 times, is placed in 75 DEG C of water-baths and soaks 2.5h, then pull an oar;
In above-mentioned raw materials, the pretreatment technology of medicinal herb components is: pulverized 40 mesh sieves after raw material mixing, mixing, obtain herbal medicine medicinal powder, add pure water, the add-on of water is about 6 times of herbal medicine medicinal powder, adopts Na
2cO
3regulate pure water pH to be about 7.8, then add and account for the cellulase that herbal medicine mass ratio is 1%, lixiviate 20min under ultrasonic assistant condition, extraction temperature about 65 DEG C, ultrasonication 6 times, each supersound process frequency is 60Hz, each sonication treatment time 3min, interval time 20s;
(7) herbal medicine fermention medium preparation: mixed by pretreated herbal medicine, adds 1.5g/L (NH
4)
2sO
4, 1.5g/LK
2hPO
4, 1g/LMgSO
4;
(8) inoculate: the yeast saccharomyces cerevisiae after step (4) being filtered inoculates herbal medicine fermention medium by the inoculum size of 10%;
(9) temperature controlled fermentation: temperature controlled fermentation under slow stirring, leavening temperature controls at 28 DEG C, and fermentation time is 36 ~ 48h;
(10) supersonic wave wall breaking: adopt ultrasonic wave mode to yeast broken wall 20min, more than mixing speed 300r/min during broken wall after fermentation ends;
(11) filter: cross after fermentation ends and filter the solid insoluble such as Chinese herb residues and fermentation thalli;
(12) ageing: for promoting the stable and clarification just proposed, is placed in ageing about 4 days under the condition of about 3 DEG C by wine liquid;
(13) allocate: the alcohol by volume concentration 12% adjusting rich selenium blueberry mixed health wine, adds 1g/L Xylitol, 5g/L potassium sorbate, 1g/L Tripotassium Citrate, 10g/L oligomeric isomaltose, 5g/L honey; Often liter of health promoting wine that " L " refers to;
(14) membrane filtration: the micro-pore-film filtration blueberry mixed health wine with aperture being 0.15 μm;
(15) sterile filling: filling and sealing obtains rich selenium blueberry mixed health wine in sterile filling production line.
Sensory evaluation scores:
Mark from following three aspects: local flavor 40 points, delicate fragrance alcohol and, aroma is strong, and all tastes is coordinated, and aroma is comparatively coordinated;
Mouthfeel 40 points, entrance is sweet, slightly tasty and refreshing bitter taste, and aftertaste is freely happy;
Structural state 20 points: as clear as crystal, uniformity, no suspended substance and throw out.
Through 50 expert analysis mode, the health promoting wine average in embodiment 2 is as follows:
Local flavor 38.85 points, mouthfeel 39.88 parts, structural state 18.08 points, adds up to score: 96.81 points.
In above-mentioned health promoting wine, the content of organoselenium is 350 μ g/L.
Resistance of oxidation is 780.25U/mL;
Select 100 women, wherein that 30-40 year is 30 people, 40-50 year be 50 people, 50-60 year be 20 people, above women has subhealth state problem in various degree, the bad black eye that cause of such as sleep quality, or the colour of skin is obscure; Or often easily suffer from the disease such as Common Cold, tonsillitis;
100 above women take health promoting wine 20mL of the present invention every night, and after one month, observe the colour of skin of these women, wherein 96 have the sign significantly having blast, and 93 sleeps take a favorable turn, and black eye alleviate;
Flu or inflammatory patients number are 0;
After two months, observe this 100 women, wherein 98 obvious blasts of skin, and also have 23 female skins to become and compact, 95 sleep states are good, and black eye alleviate;
Flu or inflammatory patients number are only 2 people.
Above-mentioned experiment shows, health promoting wine of the present invention has good antioxygenation, and the colour of skin for women highlights useful, but also improves immunity of organisms.
Embodiment 3
A production technique for rich selenium blueberry mixed health wine, it is characterized in that preparing by following method:
(1) actication of culture: rich for the high reactivity of preservation selenium yeast saccharomyces cerevisiae is activated 30min in about 37 DEG C warm water;
(2) fermented liquid preparation: preparation yeast saccharomyces cerevisiae one-step fermentation substratum, substratum glucose concn 5%, yeast extract paste concentration 1%, 121 DEG C of sterilizing 30min;
(3) one-step fermentation: rich for high reactivity after activation selenium is inoculated into fermented liquid, inoculative proportion 2%, cultivates 48h under 30 DEG C of conditions, when cultivating 12h, 24h, 36h respectively to substratum in add the Sodium Selenite of 10g/L;
(4) filter: filter after fermentation ends, be separated yeast saccharomyces cerevisiae;
(5) raw material is prepared by following portions by weight: blueberry 45 parts, matrimony vine 25 parts, Radix Et Caulis Acanthopanacis Senticosi 18 parts, Ligusticum wallichii 14 parts, Herba Epimedii 9 parts, the bighead atractylodes rhizome 9 parts, 7 parts, the dried rhizome of rehmannia, the stem of noble dendrobium 5 parts, polygala root 4 parts, Tuber Fleeceflower Root 8 parts, Root of Kirilow Rhodiola 18 parts, 8 parts, Radix Glycyrrhizae, sweet Stevia 12 parts;
(6) raw materials pretreatment:
Each raw material is taken by the parts by weight in step (5);
1. in above-mentioned raw materials, blueberry pretreatment technology is: the blueberry taking 45 parts, adds the water of 3 times, puts into 75 DEG C of water-baths and soaks 2 ~ 3h, then pull an oar;
2. the pretreatment technology of said herbal medicine raw material is: mix after raw material pulverizing, and crosses 40 mesh sieves, and obtain herbal medicine medicinal powder, add pure water, the add-on of water is about 6 times of herbal medicine medicinal powder, adopts Na
2cO
3regulate pure water pH to be about 7.5, then add and account for the cellulase that herbal medicine mass ratio is 1.5%, lixiviate 25min under ultrasonic assistant condition, extraction temperature 60 ~ 70 DEG C, obtains herbal liquid medicine;
(7) herbal medicine fermention medium preparation: by blueberry pulp after pre-treatment and herbal liquid medicine mixing, add 1.5g/L (NH in often liter of mixing solutions
4)
2sO
4, 1.5g/LK
2hPO
4, 1g/LMgSO
4;
(8) inoculate: the yeast saccharomyces cerevisiae after step (4) being filtered inoculates herbal medicine fermention medium by the inoculum size of 10%;
(9) temperature controlled fermentation: temperature controlled fermentation under slow stirring, leavening temperature controls at 26 ~ 28 DEG C, and fermentation time is 36 ~ 48h;
(10) supersonic wave wall breaking: adopt ultrasonic wave mode to yeast broken wall 30min, more than mixing speed 300r/min during broken wall after fermentation ends;
(11) filter: cross after fermentation ends and filter the solid insoluble such as Chinese herb residues and fermentation thalli;
(12) ageing: for promoting the stable and clarification just proposed, to be placed under the condition of about 4 DEG C ageing 3 days by wine liquid;
(13) allocate: the alcohol by volume concentration about 10% adjusting rich selenium blueberry mixed health wine, adds 1.2g/L Xylitol, 4g/L potassium sorbate, 1.5g/L Tripotassium Citrate, 7.5g/L oligomeric isomaltose, 7.5g/L honey;
(14) membrane filtration: the micro-pore-film filtration blueberry mixed health wine with aperture being 0.15 μm;
(15) sterile filling: filling and sealing obtains rich selenium blueberry mixed health wine in sterile filling production line.
Sensory evaluation scores:
Mark from following three aspects: local flavor 40 points, delicate fragrance alcohol and, aroma is strong, and all tastes is coordinated, and aroma is comparatively coordinated;
Mouthfeel 40 points, entrance is sweet, slightly tasty and refreshing bitter taste, and aftertaste is freely happy;
Structural state 20 points: as clear as crystal, uniformity, no suspended substance and throw out.
Through 50 expert analysis mode, the health promoting wine average in embodiment 1 is as follows:
Local flavor 39.35 points, mouthfeel 38.86 parts, structural state 18.56 points, adds up to score: 96.77 points.
In above-mentioned health promoting wine, the content of organoselenium is 350 μ g/L.
Resistance of oxidation is 753.78U/mL;
Select 100 women, wherein that 30-40 year is 30 people, 40-50 year be 50 people, 50-60 year be 20 people, above women has subhealth state problem in various degree, the bad black eye that cause of such as sleep quality, or the colour of skin is obscure; Or often easily suffer from the disease such as Common Cold, tonsillitis;
100 above women take health promoting wine 20mL of the present invention every night, and after one month, observe the colour of skin of these women, wherein 97 have the sign significantly having blast, and 92 sleeps take a favorable turn, and black eye alleviate;
Flu or inflammatory patients number are 0;
After two months, observe this 100 women, wherein 97 obvious blasts of skin, and also have 22 female skins to become and compact, 96 sleep states are good, and black eye alleviate;
Flu or inflammatory patients number are only 1 people.
Above-mentioned experiment shows, health promoting wine of the present invention has good antioxygenation, and the colour of skin for women highlights useful, but also improves immunity of organisms.
The invention provides a kind of rich selenium blueberry mixed health wine and preparation technology thereof, carried out toxicology test and the safety evaluation of rich selenium blueberry mixed health wine by animal experiment.Chmice acute Oral toxicity test-results shows, this rich selenium blueberry mixed health wine is greater than high dose group 75mg/kgbw to chmice acute per os maximum tolerated dose; Genetic toxicity test mouse marrow cell micro nuclear test and mouse inbred strain two test-results are feminine gender, show that this rich selenium blueberry mixed health wine is without mutagenesis; From rat 30d feeding study, the behavior of rat sign is normal, figure is healthy, blood and blood parameters also change without exception, and therefore the dosage (150 times of human body recommended amounts) of 75mg/kgbw belongs to safe dose; Genetic toxicity test and rat 30d feeding study result are feminine gender; Mouse experiment shows, this rich selenium blueberry mixed health wine removes lipid peroxide by GSH-Pxl enzymatic reaction, and selenium is similar with the effect of vitamin-E in growth in humans's breeding function, to maintenance organization elasticity, delay the oxidation of unsaturated fatty acids, prevent early stage aging from all having active effect.The resistance of oxidation of selenium to peroxidase exceeds 500 times than vitamin-E.Selenium and vitamin-E constitute oxidation resistant two defense pathway of animal body, and both play a role jointly, and selenium can supplement mutually with vitamin-E antioxygenation.It is not oxidized that vitamin-E controls unsaturated fatty acids on phosphatide, becomes oxidation resistant the first line of defence.Superoxide, with the reduction of the form catalysis lipid peroxide of GSH-Px, is resolved into alcohol and water rapidly by selenium in whole cell, makes the membrane structure in cell from the infringement of superoxide, plays second defence line.Therefore determine that this rich selenium blueberry mixed health wine belongs to non-toxic type food, crowd can be safe relieved edible.
The invention provides a kind of rich selenium blueberry mixed health wine and production technique thereof, wherein the product feature of blueberry mixed health wine be that the wine degree of blueberry mixed health wine is 10% ~ 12%/(
v/v), sugar degree≤15g/L, total acid (in oxysuccinic acid) 6 ~ 10g/L, volatile acid (with acetometer) <1.2g/L, sugar-free extract>=17g/L, organic selenium content 300 ~ 350 μ g/L; Blueberry mixed health wine color and luster is red-brown, crystal clear, no suspended substance and throw out; Entrance is soft pure and honest, comfortable tasty and refreshing, and all tastes are coordinated.
Above embodiment only for illustration of technical scheme of the present invention, but not is limited; Although be described in detail by invention with reference to previous embodiment, for the person of ordinary skill of the art, still the technical scheme described in previous embodiment can be modified, or equivalent replacement has been carried out to wherein portion of techniques feature; And to these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of the present invention's technical scheme required for protection.
Claims (9)
1. a production technique for rich selenium blueberry mixed health wine, comprises following step:
(1) actication of culture: activate rich selenium yeast saccharomyces cerevisiae;
(2) fermented liquid preparation: preparation yeast saccharomyces cerevisiae one-step fermentation substratum, obtains fermented liquid;
(3) ferment: the rich selenium yeast saccharomyces cerevisiae after activation in step (1) is inoculated in the fermented liquid of step (2);
(4) filter after fermentation ends, be separated yeast saccharomyces cerevisiae;
(5) mixing solutions is prepared;
Each raw material is prepared by following portions by weight:
Blueberry 40 ~ 50 parts, matrimony vine 20 ~ 30 parts, Radix Et Caulis Acanthopanacis Senticosi 15 ~ 20 parts, Ligusticum wallichii 12 ~ 15 parts, Herba Epimedii 8 ~ 10 parts, the bighead atractylodes rhizome 8 ~ 10 parts, 6 ~ 8 parts, the dried rhizome of rehmannia, the stem of noble dendrobium 4 ~ 6 parts, polygala root 3 ~ 5 parts, Tuber Fleeceflower Root 5 ~ 10 parts, Root of Kirilow Rhodiola 15 ~ 20 parts, 5 ~ 10 parts, Radix Glycyrrhizae, sweet Stevia 10 ~ 15 parts;
Blueberry pre-treatment: get blueberry, adds water, and soaks, and making beating, obtains blueberry pulp;
Herbal medicine pre-treatment: get other above-mentioned herbal component, mixing after pulverizing, add pure water, then add cellulase, lixiviate under ultrasonic assistant condition, obtains Chinese herbal medicine fluid;
(6) obtain blueberry pulp and herbal medicine liquid-phase mixing by step (5), in the mixing solutions obtained, add inorganic salts, obtain herbal medicine fermention medium;
(7) by the yeast saccharomyces cerevisiae after step (4) filtering separation by 10% inoculum size be inoculated into herbal medicine fermention medium;
(8) to the substratum fermentation obtained in step (7), supersonic wave wall breaking, filtration, ageing, allotment, membrane filtration, sterile filling, rich selenium blueberry mixed health wine is obtained.
2. the production technique of a kind of rich selenium blueberry mixed health wine as claimed in claim 1, is characterized in that, in described step (1), rich for the high reactivity of preservation selenium yeast saccharomyces cerevisiae is activated 20-40min in the water of 30 ~ 40 DEG C.
3. the production technique of a kind of rich selenium blueberry mixed health wine as claimed in claim 1, it is characterized in that, in described step (2), yeast saccharomyces cerevisiae one-step fermentation substratum in preparation steps (1), actual conditions is, glucose concn 3-6%, yeast extract paste concentration 0.6-1.5% in substratum, sterilizing 25-35min at 115-126 DEG C.
4. the production technique of a kind of rich selenium blueberry mixed health wine as claimed in claim 1, it is characterized in that, in described step (3), rich for high reactivity after activation selenium yeast saccharomyces cerevisiae is inoculated into fermented liquid, inoculative proportion 1.5-2.5%, under 28 ~ 32 DEG C of conditions, cultivate 48h, when cultivating 12h, 24h, 36h respectively to substratum in add the Sodium Selenite of 8-12g/L.
5. the production technique of a kind of rich selenium blueberry mixed health wine as claimed in claim 1, it is characterized in that, in described step (5), the pretreated concrete steps of blueberry are: take blueberry, add water, the add-on of described water is 2-5 times of blueberry weight, is placed in 65-80 DEG C of water-bath and soaks 2 ~ 3h, then pull an oar, obtain blueberry pulp;
The pretreatment technology of herbal medicine is: get remaining herbal component by following parts by weight, matrimony vine 20 ~ 30 parts, Radix Et Caulis Acanthopanacis Senticosi 15 ~ 20 parts, Ligusticum wallichii 12 ~ 15 parts, Herba Epimedii 8 ~ 10 parts, the bighead atractylodes rhizome 8 ~ 10 parts, 6 ~ 8 parts, the dried rhizome of rehmannia, the stem of noble dendrobium 4 ~ 6 parts, polygala root 3 ~ 5 parts, Tuber Fleeceflower Root 5 ~ 10 parts, Root of Kirilow Rhodiola 15 ~ 20 parts, 5 ~ 10 parts, Radix Glycyrrhizae, sweet Stevia 10 ~ 15 parts pulverized 40 mesh sieves, mixing, obtain herbal medicine medicinal powder, add water, the add-on of water is 6-10 times of Chinese herbal medicine grain weight amount, described water is the pure water after adjusting pH7.5 ~ 8.0, then cellulase is added, the add-on of described cellulase is 1 ~ 2% of Chinese herbal medicine grain weight amount, under ultrasonic assistant condition, lixiviate obtains herbal liquid medicine.
6. the production technique of a kind of rich selenium blueberry mixed health wine as claimed in claim 5, is characterized in that, in described step (5), blueberry pretreatment technology is: take blueberry, adds water, and the add-on of described water is 3 times of blueberry weight, put into 75 DEG C of water-baths and soak 2.5h, making beating, obtains blueberry pulp;
The pretreatment technology of herbal medicine is: get herbal component, pulverized 40 mesh sieves, mixing, obtain herbal medicine medicinal powder, add water, the add-on of water is 6-10 times of Chinese herbal medicine grain weight amount, described water is the pure water after adjusting pH7.8, then add cellulase, the add-on of described cellulase is 1.5% of Chinese herbal medicine grain weight amount, and under ultrasonic assistant condition, lixiviate obtains herbal liquid medicine;
The condition of described ultrasonic-assisted extraction is: ultrasonication 2-8 time, and each supersound process frequency is 20-100Hz, each sonication treatment time 2-8min, interval time 10-40s.
7. the production technique of a kind of rich selenium blueberry mixed health wine as claimed in claim 1, is characterized in that, in described step (6), inorganic salts and add-on thereof are specially: (NH
4)
2sO
41.5g/L, K
2hPO
41.5g/L and MgSO
41g/L, the add-on of above inorganic salts is the weight for adding in often liter of mixing solutions.
8. the production technique of a kind of rich selenium blueberry mixed health wine as claimed in claim 1, is characterized in that, in described step (8), fermentation step specifically, temperature controlled fermentation under slow stirring, leavening temperature controls at 26 ~ 28 DEG C, and fermentation time is 36 ~ 48h;
Adopt ultrasonic wave mode to yeast broken wall 20 ~ 30min, more than mixing speed 300r/min during broken wall after fermentation ends;
Filtering solids insolubles, obtains wine liquid;
Wine liquid to be placed under the condition of 0 ~ 10 DEG C ageing 3 ~ 5 days;
Wine liquid after adjustment ageing makes its alcohol by volume concentration be 10 ~ 12%, then adds Xylitol, potassium sorbate, Tripotassium Citrate, oligomeric isomaltose, honey in wine liquid;
Each raw material is added by following weight proportion: 1 ~ 1.5g Xylitol, 3 ~ 5g potassium sorbate, 1 ~ 2g Tripotassium Citrate, 5 ~ 10g oligomeric isomaltose, 5 ~ 10g honey in often liter of wine liquid;
Membrane filtration: micro-pore-film filtration blueberry mixed health wine;
Sterile filling: filling and sealing obtains rich selenium blueberry mixed health wine in sterile filling production line.
9. the production technique of a kind of rich selenium blueberry mixed health wine as claimed in claim 1, comprises following step:
(1) actication of culture: rich for the high reactivity of preservation selenium yeast saccharomyces cerevisiae is activated 30min in 37 DEG C of warm water;
(2) prepare fermented liquid: preparation yeast saccharomyces cerevisiae one-step fermentation substratum, in substratum, glucose concn 5%, yeast extract paste concentration 1%, 121 DEG C of sterilizing 30min, obtain fermented liquid;
(3) ferment: rich for high reactivity after activation in step (1) selenium yeast saccharomyces cerevisiae is inoculated in the fermented liquid of step (2), inoculative proportion is 2%, under 30 DEG C of conditions, cultivate 48h, when cultivating 12h, 24h, 36h respectively to substratum in add the Sodium Selenite of 10g/L;
(4) filter after fermentation ends, be separated yeast saccharomyces cerevisiae;
(5) mixing solutions is prepared;
Each raw material is prepared by following portions by weight:
Blueberry 45 parts, matrimony vine 25 parts, Radix Et Caulis Acanthopanacis Senticosi 18 parts, Ligusticum wallichii 14 parts, Herba Epimedii 9 parts, the bighead atractylodes rhizome 9 parts, 7 parts, the dried rhizome of rehmannia, the stem of noble dendrobium 5 parts, polygala root 4 parts, Tuber Fleeceflower Root 7 parts, Root of Kirilow Rhodiola 18 parts, 8 parts, Radix Glycyrrhizae, sweet Stevia 12 parts;
Blueberry pretreatment technology is: take blueberry, adds water, and the add-on of described water is 3 times of blueberry weight, puts into 75 DEG C of water-baths and soaks 2.5h, and making beating, obtains blueberry pulp;
The pretreatment technology of herbal medicine is: get herbal component, pulverized 40 mesh sieves, mixing, obtain herbal medicine medicinal powder, add water, the add-on of water is 6-10 times of Chinese herbal medicine grain weight amount, described water is the pure water after adjusting pH7.8, then add cellulase, the add-on of described cellulase is 1.5% of Chinese herbal medicine grain weight amount, lixiviate under ultrasonic assistant condition;
The condition of described ultrasonic-assisted extraction is: ultrasonication 6 times, and each supersound process frequency is 60Hz, each sonication treatment time 3min, interval time 20s;
(6) mixing obtaining blueberry pulp in step (5) with Chinese herbal medicine liquid phase, in the mixing solutions obtained, adding inorganic salts, obtain herbal medicine fermention medium;
(7) yeast saccharomyces cerevisiae after filtration in step (4) is pressed the inoculum size inoculation herbal medicine fermention medium of 10%;
(8) fermentation, supersonic wave wall breaking, filtration, ageing, allotment, membrane filtration, sterile filling, obtains rich selenium blueberry mixed health wine;
Concrete steps are as follows: temperature controlled fermentation under slow stirring, and leavening temperature controls at 26 ~ 28 DEG C, and fermentation time is 36 ~ 48h;
Adopt ultrasonic wave mode to yeast broken wall 20 ~ 30min after fermentation ends, mixing speed 300-500r/min during broken wall;
Filtering solids insolubles, obtains wine liquid;
Wine liquid to be placed under 5 DEG C of conditions ageing 4 days;
Wine liquid after adjustment ageing makes its alcohol by volume concentration 11%, then adds Xylitol, potassium sorbate, Tripotassium Citrate, oligomeric isomaltose, honey in wine liquid;
Each raw material is added in following ratio: 1.2g Xylitol, 4g potassium sorbate, 1.5g Tripotassium Citrate, 8g oligomeric isomaltose, 8g honey in often liter of wine liquid;
Membrane filtration: the micro-pore-film filtration blueberry mixed health wine with aperture being 0.15 μm;
Sterile filling: filling and sealing obtains rich selenium blueberry mixed health wine in sterile filling production line.
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| CN105820912A (en) * | 2016-05-05 | 2016-08-03 | 安徽味多多餐饮管理有限公司 | Selenium-rich blueberry healthcare wine and preparing method thereof |
| CN105820899A (en) * | 2016-04-25 | 2016-08-03 | 芜湖市好亦快食品有限公司三山分公司 | Water caltrop leaf eyesight-improving honey wine and preparation method thereof |
| CN106036490A (en) * | 2016-05-26 | 2016-10-26 | 长沙湘资生物科技有限公司 | Blueberry natto and preparation method thereof |
| CN106221994A (en) * | 2016-08-01 | 2016-12-14 | 王永怡 | Utilize the process of Chinese herbal medicine brewing health-care wine |
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| CN105820899A (en) * | 2016-04-25 | 2016-08-03 | 芜湖市好亦快食品有限公司三山分公司 | Water caltrop leaf eyesight-improving honey wine and preparation method thereof |
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| CN106036490A (en) * | 2016-05-26 | 2016-10-26 | 长沙湘资生物科技有限公司 | Blueberry natto and preparation method thereof |
| CN106221994A (en) * | 2016-08-01 | 2016-12-14 | 王永怡 | Utilize the process of Chinese herbal medicine brewing health-care wine |
| CN106307491A (en) * | 2016-08-19 | 2017-01-11 | 铜仁市金农绿色农业科技有限公司 | Method for preparing dendrobium officinale enzymes |
| CN107312680A (en) * | 2017-08-23 | 2017-11-03 | 董军 | A kind of selenium-rich blueberry mixed health wine |
| CN108559713A (en) * | 2017-12-28 | 2018-09-21 | 广东顺德酒厂有限公司 | A kind of saccharomyces cerevisiae and its application |
| CN108559713B (en) * | 2017-12-28 | 2021-08-24 | 广东顺德酒厂有限公司 | Saccharomyces cerevisiae and application thereof |
| CN108165410A (en) * | 2018-02-07 | 2018-06-15 | 浦城县元之蓝家庭农场 | A kind of blueberry wine and preparation method thereof |
| CN111218367A (en) * | 2020-03-09 | 2020-06-02 | 杭州汇之宝农业科技有限公司 | Production process of selenium-rich blueberry health wine |
| CN111357988A (en) * | 2020-04-22 | 2020-07-03 | 智昌药业(云南)有限公司 | Selenium-rich composite enzyme and preparation process thereof |
| CN116162518A (en) * | 2022-12-13 | 2023-05-26 | 西北农林科技大学 | Medlar wine rich in organic selenium and preparation method thereof |
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