CN105475986A - Preparation method of fermented yak bone paste - Google Patents
Preparation method of fermented yak bone paste Download PDFInfo
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- CN105475986A CN105475986A CN201510903302.4A CN201510903302A CN105475986A CN 105475986 A CN105475986 A CN 105475986A CN 201510903302 A CN201510903302 A CN 201510903302A CN 105475986 A CN105475986 A CN 105475986A
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Abstract
The invention discloses a preparation method of fermented yak bone paste. The preparation method is used for solving a difficult problem that absorptivity of calcium in yak bone paste is low, and fermentation function of compound lactobacillus is adopted so as to change calcium in yak bone past into ionized calcium which can be easily absorbed by human body. The preparation method comprises following steps: yak bone is made into bone paste with a particle size ranging from 100 to 200 meshes; sucrose and distilled water are added so as to prepare a fermentation liquid culture medium; the fermentation liquid culture medium is inoculated with 2 to 8% of compound lactobacillus after autoclaved sterilization and cooling; and fermentation is carried out for 24 to 48h in a incubator at 36 to 42 DEG C. The content of ionized calcium in the fermented yak bone paste is increased obviously; operation of the preparation method is simple; production period is short; production cost is low; quality is stable; and industrialized production can be realized easily.
Description
Technical field
The present invention relates to fermentation bone mud field of food, particularly relate to a kind of preparation method of the yak bone paste food that ferments.
Background technology
The yak resources of China enriches, and accounts for more than 90% of whole world yak sum, ranks first in the world.In today of pasture animal husbandry fast development, a large amount of Yak Bone is not used, and not only wastes resource but also cause huge pollution to environment.Yak Bone is growth and development stage Children and teenager, and the calcium nutrient supplementary source that middle-aged and senile osteoporosis patient is desirable.Fresh bone paste is the food source that China newly opens up in recent years, containing the nutritional labeling that many needed by human body are wanted, as protein, fat, vitamin, amino acid, ossein, calcium phosphorus etc.But people mainly utilize bone mud by the method for Physical Processing at present, through the bone mud of processing process like this, the absorptivity of human body to its absorptivity especially calcium is lower, is unfavorable for absorption of human body.Therefore this project adopts the method for lactobacillus-fermented to produce yak bone paste, and the chemical combination calcium in bone mud is changed be easy to into the ionized calcium that body absorbs, protein portion is converted into amino acid, thus improves its absorption rate.Be not only the comprehensive utilization of Yak Bone like this and develop ecological supplementation product and open up a new way, greatly improving the economic benefit of enterprise simultaneously, reduce the pollution to environment, thus there is good social benefit.
Summary of the invention
Technical problem to be solved by this invention is the difficult problem that the calcium absorptivity in bone mud is low, thus provides a kind of method utilizing the Fermentation Function of lactic acid bacteria to prepare fermentation yak bone paste.
The present invention adopts following technical scheme:
1. the preparation of yak bone paste: the Yak Bone choosing fresh clean, is cut into the bone block of suitable size by bone with cutting machine; Clear water cleans Yak Bone, and unhairing, dehematize dirt and other foreign matter, put into freezer at-20 DEG C of freezing more than 12h after rejecting broken bone, fat, ligament, periosteum; Be first the fritter of diameter 5mm ~ 10mm by Yak Bone coarse crushing with bone cutter, carry out fine powder with colloid mill again and be broken into bone mud, then bone mud is dropped into supermicro mill fine grinding, with to add when colloid mill and supermicro mill process bone mud simultaneously a certain amount of ice bits, control bone mud and be in low temperature, obtain the yak bone paste that granularity is 100 ~ 200 object ultramicro grinding.
2. the activation of lactic acid bacteria: accessed by compound lactobacillus in the MRS fluid nutrient medium of sterilizing respectively, cultivates that to insert refrigerator cold-storage after 12h for subsequent use for 40 DEG C.
3. yak bone paste zymotic fluid medium component is made up of fresh bone paste, sucrose and distilled water.
4. the fermentation of yak bone paste: by 4 kinds of compound lactobacillus with 2 ~ 8% ratio be aseptically inoculated in the good zymotic fluid of sterilizing, fresh bone paste 5 ~ 30% in zymotic fluid, sucrose 5 ~ 10%, 36 ~ 42 DEG C in incubator ferment 24 ~ 48h.
5. the primary quality measure of the fermentation yak bone paste obtained: the content of free calcium and amino-acid nitrogen.
6. assay method: free calcium catalyst adopts atomic absorption spectrophotometry; The mensuration of amino-acid nitrogen adopts potentiometric titration.
7. compared with the conventional method, the present invention uses compound lactobacillus-fermencucumber yak bone paste first, and the fermentation yak bone paste that this kind of method obtains takes full advantage of the advantage of lactic acid bacteria and yak bone paste, and the pH of goods is reduced, and the content of free calcium and amino-acid nitrogen raises.The technology of the present invention is simple to operate, with short production cycle, and cost is low, and steady quality easily realizes industrialization.
Detailed description of the invention
For a better understanding of the present invention, further illustrate below in conjunction with specific embodiment.
Embodiment 1:
(1) preparation of zymotic fluid: 100 order yak bone pastes 15%, sucrose 5%, water 80%, total fermentating liquid volume 100%, 121 DEG C of autoclaving 15min, are cooled to preference temperature for subsequent use;
(2) to ferment yak bone paste preparation: compound lactobacillus, numbering YF-L811, according to the ratio aseptic inoculation of inoculum concentration 3% in zymotic fluid, fermentation temperature 40 DEG C, fermentation time 24h.Measuring bone mud Free Calcium content after fermentation is 460.9mg/100mg, is 43 times of the front free calcium levels that ferments; The content of amino-acid nitrogen is 94.3mg/100mg, is 1.12 times before fermentation.
Embodiment 2:
(1) preparation of zymotic fluid: 100 order yak bone pastes 15%, sucrose 5%, water 80%, total fermentating liquid volume 100%, 121 DEG C of autoclaving 15min, are cooled to preference temperature for subsequent use;
(2) to ferment yak bone paste preparation: compound lactobacillus, numbering YF5700T, according to the ratio aseptic inoculation of inoculum concentration 3% in zymotic fluid, fermentation temperature 40 DEG C, fermentation time 24h.Measuring bone mud Free Calcium content after fermentation is 382.3mg/100mg, is 34 times of the front free calcium levels that ferments; The content of amino-acid nitrogen is 130.4mg/100mg, is 1.54 times before fermentation.
Embodiment 3:
(1) preparation of zymotic fluid: 150 order yak bone pastes 15%, sucrose 8%, water 77%, total fermentating liquid volume 100%, 121 DEG C of autoclaving 15min, are cooled to preference temperature for subsequent use;
(2) to ferment yak bone paste preparation: compound lactobacillus, code T S-H1346, according to the ratio aseptic inoculation of inoculum concentration 3% in zymotic fluid, fermentation temperature 38 DEG C, fermentation time 36h.Measuring bone mud Free Calcium content after fermentation is 687.25mg/100ml, is 64 times of the front free calcium levels that ferments; The content of amino-acid nitrogen is 144.8mg/100g, is 1.71 times before fermentation.
Embodiment 4:
(1) preparation of zymotic fluid: 150 order yak bone pastes 20%, sucrose 5%, water 75%, total fermentating liquid volume 100%, 121 DEG C of autoclaving 15min, are cooled to preference temperature for subsequent use;
(2) to ferment yak bone paste preparation: compound lactobacillus, code T S-H1346, according to the ratio aseptic inoculation of inoculum concentration 3% in zymotic fluid, fermentation temperature 38 DEG C, fermentation time 48h.Bone mud Free Calcium is measured after fermentation
Content is 482.3mg/100mg, is 59 times of the front free calcium levels that ferments; The content of amino-acid nitrogen is 122.4mg/100mg, is 1.59 times before fermentation.
Embodiment 5:
(1) preparation of zymotic fluid: 100 order yak bone pastes 15%, sucrose 5%, water 80%, total fermentating liquid volume 100%, 121 DEG C of autoclaving 15min, are cooled to preference temperature for subsequent use;
(2) to ferment yak bone paste preparation: compound lactobacillus, numbering YO-MIX300, according to the ratio aseptic inoculation of inoculum concentration 3% in zymotic fluid, fermentation temperature 40 DEG C, fermentation time 36h.Measuring bone mud Free Calcium content after fermentation is 582.3mg/100mg, is 54 times of the front free calcium levels that ferments; The content of amino-acid nitrogen is 134.4mg/100mg, is 1.58 times before fermentation.
Embodiment 6:
(1) preparation of zymotic fluid: 200 order yak bone pastes 10%, sucrose 8%, water 82%, total fermentating liquid volume 100%, 121 DEG C of autoclaving 15min, are cooled to preference temperature for subsequent use;
(2) to ferment yak bone paste preparation: compound lactobacillus, code T S-H1346, according to the ratio aseptic inoculation of inoculum concentration 3% in zymotic fluid, fermentation temperature 38 DEG C, fermentation time 48h.Measuring bone mud Free Calcium content after fermentation is 593.3mg/100mg, is 48 times of the front free calcium levels that ferments; The content of amino-acid nitrogen is 128.4mg/100mg, is 1.09 times before fermentation.
Claims (6)
1. the preparation method of the yak bone paste that ferments, it is characterized in that: Yak Bone being made granularity is 100 ~ 200 object bone mud, add sucrose and distilled water makes zymotic fluid, the compound lactobacillus of inoculation 2 ~ 8% after the cooling of zymotic fluid autoclaving, ferment 24 ~ 48h in the incubator of 36 ~ 42 DEG C.
2. the preparation method of fermentation yak bone paste according to claim 1, is characterized in that: the described yak bone paste handled well obtains by the following method: the Yak Bone choosing fresh clean, with cutting machine, bone is cut into the bone block of suitable size; Clear water cleans Yak Bone, and unhairing, dehematize dirt and other foreign matter, put into freezer at-20 DEG C of freezing more than 12h after rejecting broken bone, fat, ligament, periosteum; Be first the fritter of diameter 5mm ~ 10mm by Yak Bone coarse crushing with bone cutter, carry out fine powder with colloid mill again and be broken into bone mud, then bone mud is dropped into supermicro mill fine grinding, with to add when colloid mill and supermicro mill process bone mud simultaneously a certain amount of ice bits, control bone mud and be in low temperature, obtain the yak bone paste that granularity is 100 ~ 200 object ultramicro grinding.
3. the preparation method of fermentation yak bone paste according to claim 1, is characterized in that: zymotic fluid used is made up of yak bone paste, sucrose and distilled water.
4. the preparation method of the fermentation yak bone paste according to claim 1 or 3, is characterized in that: in zymotic fluid, the ratio of yak bone paste is 5 ~ 30%, and the ratio of sucrose is 5 ~ 10%.
5. the preparation method of the fermentation yak bone paste according to claim, is characterized in that: the sterilising conditions of zymotic fluid culture medium is: 121 DEG C, 15min.
6. the preparation method of fermentation yak bone paste according to claim 1, is characterized in that: the activation of compound lactobacillus is by being inoculated in the MRS fluid nutrient medium of sterilizing, cultivates 12h for 40 DEG C.
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| CN201510903302.4A CN105475986A (en) | 2015-12-09 | 2015-12-09 | Preparation method of fermented yak bone paste |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106490494A (en) * | 2016-10-12 | 2017-03-15 | 内蒙古蒙肽生物工程有限公司 | Produce the process of bone mud fermentation liquid |
| CN112450428A (en) * | 2020-10-23 | 2021-03-09 | 内蒙古蒙肽生物工程有限公司 | Bone mud fermentation liquor and preparation method thereof |
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2015
- 2015-12-09 CN CN201510903302.4A patent/CN105475986A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106490494A (en) * | 2016-10-12 | 2017-03-15 | 内蒙古蒙肽生物工程有限公司 | Produce the process of bone mud fermentation liquid |
| CN112450428A (en) * | 2020-10-23 | 2021-03-09 | 内蒙古蒙肽生物工程有限公司 | Bone mud fermentation liquor and preparation method thereof |
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Application publication date: 20160413 |