CN105462948A - Wheat based diet specific xylan degradation compound enzyme preparation and preparation method and application thereof - Google Patents
Wheat based diet specific xylan degradation compound enzyme preparation and preparation method and application thereof Download PDFInfo
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Abstract
The invention relates to a wheat based diet specific xylan degradation compound enzyme preparation. The active component of the wheat based diet specific xylan degradation compound enzyme preparation comprises xylanase and arabinfuranosidease and/or ferulic acid esterase. The invention aims at preparing the wheat based diet specific araboxylan degradation compound enzyme preparation according to the structural features of the wheat araboxylan, and the utilization effect of poultry to wheat is targetedly improved. By means of the specific xylan degradation compound enzyme preparation, the utilization effect of the wheat is improved, proliferation of beneficial bacteria such as intestinal lactic acid bacteria and the like is further facilitated, intestinal health is achieved, and the production performance of the poultry is greatly improved.
Description
Technical field
The invention belongs to livestock and poultry cultivation and feed-formulating technique field, be specifically related to a kind of Wheat Based Diet specificity xylan degrading compound enzymic preparation and its preparation method and application.
Background technology
China is livestock and poultry cultivation big country, along with the development of China's aquaculture, needs every year to consume a large amount of mixed feeds.Within 2014, China's fodder production amount reaches 1.5 ten thousand tons.Although wheat is mainly as the food sources of China resident, its protein content is high, usually has price advantage compared with corn, often as the common feedstuffs raw material of livestock and poultry, is widely used in the production of animal and fowl fodder.In the world, Europe, the country that the corn seeding areas such as Australia are less, wheat is also used to one of animal and fowl fodder raw material in a large number.Although wheat comparatively corn nourishment is worth high, wheat is rich in more antinutritional factor, mainly araboxylan (arabinoxylan, AX).Araboxylan comprises main chain and branched structure; main chain is through β-1 by wood sugar; 4-glycosidic link is formed by connecting, and branched structure mainly comprises pectinose (L-arabinose), ethanoyl (acetyl), forulic acid (ferulicacid), glucuronic acid (glucuronicacid), tonka bean camphor (p-coumaric) etc.
Owing to lacking the gene of expressing related degradation enzyme in livestock and poultry body, livestock and poultry self can not be degraded araboxylan.Araboxylan enters after in livestock and poultry body can form viscous chyme, causes the intestinal health problems such as pathogenic bacteria propagation and intestinal inflammation, reduces the utilization of nutrient, and then reduce the production performance of livestock and poultry.Therefore, screen and utilize suitable exogenous enzyme preparations to be zootrophic main direction of studying to the araboxylan of degrading in wheat always.At present, to degrade araboxylan by adding zytase in Wheat-Based Diets, the utilizing status improving Wheat Based Diet has become a kind of general way.But for the zytase that the zymin of wheat is mainly single on market, or by zytase and other carbohydrases, or proteolytic enzyme is composite forms prozyme together.
In the cereal such as wheat, main branched structure is arbinofuranose and forulic acid.Much research shows, these branched structures can by steric restriction effect, reduces zytase to the Degradation of araboxylan, and make the action effect of zytase and imperfection, the araboxylan major part in wheat is insoluble.
Degradable product due to araboxylan is Arabic xylo-oligosaccharide (arabixylanoligosaccharides, AXOS), and it is a kind of novel prebiotics, effectively can promote probiotic bacterium, as milk-acid bacteria, and the propagation of bifidus bacillus etc.There are some researches show, its prebiotic effect is even better than oligofructose.Therefore, how the araboxylan in the cereal such as wheat is effectively decomposed, while eliminating its antinutritional factor, generate more oligose, promote the probiotics propagation such as milk-acid bacteria, regulate and control the intestinal health of livestock and poultry, cause the extensive concern of people.In addition, zytase effect in vivo is also subject to feed manufacturing, the impact of the many factors such as animal varieties and feeding environment.Therefore, the hot issue that the action effect of zytase in livestock and poultry body has become current nutrition and zymin research how is improved.
Due to the specificity of araboxylan structure, single zytase effectively can not decompose araboxylan.And most of araboxylan is mainly insolubility in wheat.Therefore, low to the degradation efficiency of araboxylan in single zytase wheat.Chinese patent publication No. CN103725662A discloses a kind of Wheat ration enzyme containing alkalescent xylanase and preparation method thereof, although it considers the weakly alkaline environment in animal and bird intestines, but this invention still contains only single zytase, not containing the enzyme had for araboxylan branched structure.Therefore, not pointed to the structure of araboxylan, its degradation efficiency is still not high.
Summary of the invention
For above-mentioned technical problem, the present invention seeks to the constructional feature according to Wheat Arabinoxylan, the specific araboxylan degradation complex enzyme preparation of preparation Wheat Based Diet, improves the utilizing status of poultry to wheat targetedly.The present invention, by adopting Idiotype xylan compound enzymic preparation, not only improves the utilizing status of wheat, further promotes the propagation of the probioticss such as enteron aisle milk-acid bacteria, realize intestinal health, substantially improve the production performance of poultry.
Realize the object of the invention technical scheme as follows:
A kind of Wheat Based Diet specificity xylan degrading compound enzymic preparation, its activeconstituents comprises zytase, also comprises arabinofuranosidase and/or feruloyl esterase.
Preferably, above-mentioned Wheat Based Diet specificity xylan degrading compound enzymic preparation, its activeconstituents comprises zytase 11250-15000U/g, also comprises arabinofuranosidase 2080-3120U/g and/or feruloyl esterase 160-240U/g.
Further preferably, above-mentioned Wheat Based Diet specificity xylan degrading compound enzymic preparation, its activeconstituents comprises zytase 12000-13750U/g, also comprises arabinofuranosidase 2340-2860U/g and/or feruloyl esterase 180-220U/g.
Preferably, above-mentioned Wheat Based Diet specificity xylan degrading compound enzymic preparation, its activeconstituents comprises zytase 12500U/g, also comprises arabinofuranosidase 2600U/g and/or feruloyl esterase 200U/g.
Above-mentioned Wheat Based Diet specificity xylan degrading compound enzymic preparation, also comprises thinner.
Preferably, described thinner can be this area such as flour, defatted rice bran can thinner.
Preferably, described zytase is described zytase is β-Isosorbide-5-Nitrae-endo-xylanase.
Preferably, described zytase derives from Rumen Fungi (Neocallimastixpatriciarum), arabinofuranosidase derives from bacillus pumilus (Bacilluspumilus), feruloyl esterase derives from Clostridium thermocellum (Clostridiumthermocellum).
Zytase of the present invention, arabinofuranosidase, feruloyl esterase all can be obtained by existing ordinary method; Also can purchased from commercial goods product.
Alternatively, the enzyme activity unit of described zytase, arabinofuranosidase, feruloyl esterase is respectively 100000U/g, 4000U/g, 3200U/g.
The present invention also provides the preparation method of above-mentioned Wheat Based Diet specificity xylan degrading compound enzymic preparation, and described method comprises each raw material is mixed by prior art ordinary method and be get final product.Such as, described preparation method comprises and gets various enzyme as activeconstituents by proportioning, and/or adds thinner, mixes and get final product.
Particularly, the preparation method of above-mentioned Wheat Based Diet specificity xylan degrading compound enzymic preparation, comprise the following steps: get various enzyme as activeconstituents by proportioning, get 25g zytase (100000U/g), it is 200g that 130g arabinofuranosidase (4000U/g) and/or 12.5g feruloyl esterase (3200U/g) add defatted rice bran thinner total amount, mixes and get final product.
The present invention also comprises the application of above-mentioned compound enzymic preparation in Wheat Based Diet feed formulation.
Compound enzymic preparation of the present invention is applicable to feed-processing plant and plant's autogamy feed.Should mix with other raw material in feed during use, can in advance compound enzymic preparation of the present invention be mixed with a small amount of feed, remix in large quantities of feed, Direct-fed.Advise that the addition of described compound enzymic preparation in Wheat Based Diet feed is 100-200g/ ton; Preferably, when in Wheat Based Diet feed, wheat weighs less than 30%, described compound enzymic preparation addition is 100g/ ton; When Wheat Based Diet feed wheat weight is more than 30%, described compound enzymic preparation addition is 200g/ ton.
Beneficial effect
The present invention is mainly for the constructional feature of araboxylan in the grain ration such as wheat, utilize araboxylan side chain lytic enzyme can its branched structure of specific degraded, eliminate the steric restriction effect of branched structure, increase the affinity of zytase and araboxylan.Therefore, araboxylan side chain lytic enzyme and zytase compatibility are used, synergy can be played, promote the degraded of araboxylan, and generate more xylo-oligosaccharide, promote the propagation of intestinal beneficial bacterium especially milk-acid bacteria, suppress pathogenic bacteria, improve poultry intestinal tract environment, and improve intestinal health, thus improve the production performance of broiler chicken.
Advantage of the present invention:
(1) be used alone compared with zytase, the present invention can degrade araboxylan further, generates more xylo-oligosaccharide, promotes the increment of probiotics.
(2) for Wheat Based Diet, be comparatively used alone zytase, the present invention further increases the production performance of broiler chicken.
(3) be used alone compared with zytase, the present invention can also reduce Intestine of Broiler damage further, reduces enteron aisle chyme stickiness and pH value, improves the intestinal health of poultry.
Social benefit and economic benefit
1, main application: the poultry such as livestock and poultry use the zymin of adding during Wheat Based Diet, are particularly useful for broiler chicken, laying hen, meat duck, egg duck use the compound enzymic preparation added during wheat.
2, application benefit: with wheat be raw material configuration daily ration of broiler in add compound enzymic preparation of the present invention, make the average daily gain of broiler chicken 36d comparatively control group bring up to 55.04g by 48.97g; Feed-weight ratio drops to 1.77 by 1.97.And the average daily gain of interpolation xylanase treatment group brings up to 55.04g by 51.24g more separately, improves 12.40%; Feed-weight ratio is reduced to 1.77 by 1.88, reduces by 10.15%.The result of use of compound enzymic preparation of the present invention is better than being used alone zytase.
3, promotional value: in recent years, wheat is widely used as energy feed.At 2012-2013, China's mixed feed, pig, poultry, egg fowl consume wheat and by product 2,339 ten thousand, 1,986 ten thousand, 8,370,000,8,630,000 tons thereof respectively.But because wheat and by product thereof are rich in a large amount of antinutritional factor, mainly araboxylan.Therefore, the use of zymin, the especially interpolation of zytase, particularly important.Zytase for the healthy state improving livestock and poultry, reduces the death that diarrhoea and intestinal tract injury cause, for ensureing that livestock birds health cultivation has great importance in the interpolation of Wheat Based Diet.
According to China's feed enzyme preparation market analysis and Potential Prediction report (2014-2018), China's fodder enzyme preparation turnout will reach 200,000 tons.Zytase product is one of main zymin kind, although each zymin in market company produces numerous zytase products for the araboxylan in wheat in recent years, its homogeneity is comparatively strong, is all single zytase product.Zytase for be the backbone structure of araboxylan, and branched structure of can not degrading, its effect is comparatively limited.
Along with the expansion of China's broiler breeding scale, the usage quantity of aquaculture to wheat also increases thereupon.Therefore, promote the specificity arabinoxylanase preparation designed for Wheat Arabinoxylan structure, there are good practical value and wide market outlook.
Accompanying drawing explanation
Fig. 1 is the electron-microscope scanning figure of destarching wheat bran after different enzymolysis processing in experimental example 1.
Embodiment
Following examples for illustration of the present invention, but are not used for limiting the scope of the invention.Unreceipted concrete technology or condition person in embodiment, according to the technology described by the document in this area or condition, or carry out according to product description.Agents useful for same or the unreceipted production firm person of instrument, be and purchase available conventional products by regular distributor.Described in following examples and experimental example, zytase, arabinofuranosidase, feruloyl esterase derive from Rumen Fungi (Neocallimastixpatriciarum), bacillus pumilus (Bacilluspumilus), Clostridium thermocellum (Clostridiumthermocellum) respectively; Enzyme activity unit is respectively 100000U/g, 4000U/g, 3200U/g.
Embodiment 1
A kind of Wheat Based Diet specificity xylan degrading compound enzymic preparation, its activeconstituents comprises zytase 12500U/g, arabinofuranosidase 2600U/g, feruloyl esterase 200U/g; Wherein said zytase is β-Isosorbide-5-Nitrae-endo-xylanase.
The present embodiment also provides the preparation method of this Wheat Based Diet specificity xylan degrading compound enzymic preparation, comprise: get various enzyme as activeconstituents by proportioning, get 25g zytase, it is 200g that 130g arabinofuranosidase and 12.5g feruloyl esterase add defatted rice bran thinner to total amount, mixes and get final product.
Embodiment 2
A kind of Wheat Based Diet specificity xylan degrading compound enzymic preparation, its activeconstituents comprises zytase 11250U/g, arabinofuranosidase 2080U/g, feruloyl esterase 160U/g; Wherein said zytase is β-Isosorbide-5-Nitrae-endo-xylanase.
The present embodiment also provides the preparation method of this Wheat Based Diet specificity xylan degrading compound enzymic preparation, comprises the following steps: get various enzyme as activeconstituents by proportioning, mix and get final product.
Embodiment 3
A kind of Wheat Based Diet specificity xylan degrading compound enzymic preparation, its activeconstituents comprises zytase 15000U/g, arabinofuranosidase 3120U/g, feruloyl esterase 240U/g; Wherein said zytase is β-Isosorbide-5-Nitrae-endo-xylanase.
The present embodiment also provides the preparation method of this Wheat Based Diet specificity xylan degrading compound enzymic preparation, comprises the following steps: get various enzyme as activeconstituents by proportioning, mix and get final product.
Embodiment 4
A kind of Wheat Based Diet specificity xylan degrading compound enzymic preparation, its activeconstituents comprises zytase 13750U/g, arabinofuranosidase 2860U/g, feruloyl esterase 220U/g; Wherein said zytase is β-Isosorbide-5-Nitrae-endo-xylanase; This compound enzymic preparation also comprises thinner defatted rice bran.
The present embodiment also provides the preparation method of this Wheat Based Diet specificity xylan degrading compound enzymic preparation, comprises the following steps: get various enzyme as activeconstituents by proportioning, add thinner defatted rice bran, mix and get final product.
Embodiment 5
A kind of Wheat Based Diet specificity xylan degrading compound enzymic preparation, its activeconstituents comprises zytase 12000U/g, arabinofuranosidase 2340U/g, feruloyl esterase 180U/g; Wherein said zytase is β-Isosorbide-5-Nitrae-endo-xylanase; This compound enzymic preparation also comprises thinner flour.
The present embodiment also provides the preparation method of this Wheat Based Diet specificity xylan degrading compound enzymic preparation, comprises the following steps: get various enzyme as activeconstituents by proportioning, add thinner flour, mix and get final product.
Embodiment 6
A kind of Wheat Based Diet specificity xylan degrading compound enzymic preparation, its activeconstituents comprises zytase 12500U/g, arabinofuranosidase 2600U/g; Wherein said zytase is β-Isosorbide-5-Nitrae-endo-xylanase.
Embodiment 7
A kind of Wheat Based Diet specificity xylan degrading compound enzymic preparation, its activeconstituents comprises zytase 12500U/g, feruloyl esterase 200U/g; Wherein said zytase is β-Isosorbide-5-Nitrae-endo-xylanase.
Embodiment 8
A kind of Wheat Based Diet specificity xylan degrading compound enzymic preparation, its activeconstituents comprises zytase 11250U/g, feruloyl esterase 160U/g; Wherein said zytase is β-Isosorbide-5-Nitrae-endo-xylanase.
Embodiment 9
A kind of Wheat Based Diet specificity xylan degrading compound enzymic preparation, its activeconstituents comprises zytase 12000U/g, arabinofuranosidase 2340U/g; Wherein said zytase is β-Isosorbide-5-Nitrae-endo-xylanase.
Embodiment 10
A kind of Wheat Based Diet specificity xylan degrading compound enzymic preparation, its activeconstituents comprises zytase 15000U/g, feruloyl esterase 240U/g; Wherein said zytase is β-Isosorbide-5-Nitrae-endo-xylanase.
Embodiment 11
A kind of Wheat Based Diet specificity xylan degrading compound enzymic preparation, its activeconstituents comprises zytase 15000U/g, the wherein said zytase of arabinofuranosidase 3120U/g is β-Isosorbide-5-Nitrae-endo-xylanase.
Embodiment 12
A kind of Wheat Based Diet specificity xylan degrading compound enzymic preparation, its activeconstituents comprises zytase 13750U/g, feruloyl esterase 220U/g; Wherein said zytase is β-Isosorbide-5-Nitrae-endo-xylanase; This compound enzymic preparation also comprises thinner defatted rice bran.
Embodiment 13
A kind of Wheat Based Diet specificity xylan degrading compound enzymic preparation, its activeconstituents comprises zytase 13750U/g, arabinofuranosidase 2860U/g; Wherein said zytase is β-Isosorbide-5-Nitrae-endo-xylanase; This compound enzymic preparation also comprises thinner defatted rice bran.
Embodiment 14
A kind of Wheat Based Diet specificity xylan degrading compound enzymic preparation, its activeconstituents comprises zytase 11250U/g, feruloyl esterase 160U/g; Wherein said zytase is β-Isosorbide-5-Nitrae-endo-xylanase; This compound enzymic preparation also comprises thinner flour.
Embodiment 15
A kind of Wheat Based Diet specificity xylan degrading compound enzymic preparation, its activeconstituents comprises zytase 11250U/g, arabinofuranosidase 2080U/g; Wherein said zytase is β-Isosorbide-5-Nitrae-endo-xylanase; This compound enzymic preparation also comprises thinner flour.
Described in embodiment 6-15, Wheat Based Diet specificity xylan degrading compound enzymic preparation is prepared from all according to a conventional method.
Experimental example 1
The present invention, according to the structure of araboxylan in wheat, designs the compound enzymic preparation of selective degradation araboxylan.By using wheat solubility araboxylan, soluble araboxylan, beech xylan and destarching wheat bran as substrate, by synchronism synergy and continuity synergy mode, research zytase and arabinofuranosidase, the synergy of feruloyl esterase when degrading araboxylan.Result shows, arabinofuranosidase and feruloyl esterase can promote that zytase is to the Degradation of araboxylan further, promotes the release (experimental result is in table 1) of reducing sugar.
The synergy of table 1. zytase and arabinofuranosidase, feruloyl esterase
X: zytase; A: arabinofuranosidase; F: feruloyl esterase; DS: cofactor.
In addition, for destarching wheat bran, the synergy of three kinds of zymins can destroy the structure on wheat bran surface further, and make cellular structure more obvious, experimental result is shown in Fig. 1.Fig. 1 is the electron-microscope scanning figure of destarching wheat bran after different enzymolysis processing, wherein a: contrast (need not any ferment treatment); B: zytase; C: zytase+arabinofuranosidase; D: zytase+feruloyl esterase; E: zytase+arabinofuranosidase+feruloyl esterase.Enzymolysis processing process is add three kinds of enzymes respectively in the substrate (destarching wheat bran) of 1% in mass concentration, and in each process end reaction system, zymin enzyme is lived as follows: b: zytase 25U/mL; C: zytase 25U/mL+ arabinofuranosidase 5.2U/mL; D: zytase 25U/mL+ feruloyl esterase 0.4U/mL; E: zytase 25U/mL+ arabinofuranosidase 5.2U/mL+ feruloyl esterase 0.4U/mL.
By the hydrolysising product analysis to araboxylan, result shows comparatively to be used alone zytase, and the compatibility of three kinds of zymins uses and can promote that araboxylan is hydrolyzed further, and generate more xylo-oligosaccharide, experimental result is in table 2.
The product composition of table 2. Wheat Arabinoxylan after different enzymolysis processing
X: zytase; A: arabinofuranosidase; F: feruloyl esterase.
In addition, tested by the In Vitro Fermentation of araboxylan hydrolysate, result shows, araboxylan is after the synergy of three kinds of zymins, its hydrolysate has better prebiotic effect, effectively can promote the propagation (table 3, table 4) of subtilis and milk-acid bacteria.
Table 3. solubility araboxylan is after different enzymolysis processing
athe prebiotic effect of product
Table 4. insolubility araboxylan is after different enzymolysis processing
bthe prebiotic effect of product
Each symbol in table 3, table 4, alphabetical implication are as follows:
X: zytase; A: arabinofuranosidase; F: feruloyl esterase
A solubility araboxylan through zytase and itself and arabinofuranosidase, feruloyl esterase hydrolytic action;
B insolubility araboxylan through zytase and itself and arabinofuranosidase, feruloyl esterase hydrolytic action;
Glu: glucose; FOS: Nutriflora P; WE-AX: refer to solubility araboxylan, insolubility araboxylan in table 3, table 4 respectively.
*, allometry (to glucose);
-, can not grow; +, 1% – 10% grows; ++, 11% – 20% grows;
+++, 21% – 30% grows; ++++, 31% – 40% grows;
+++ ++, 41% – 60% grows.
Experimental example 2 improves the utilization ratio of broiler chicken to wheat
This experiment is by selecting the male broiler chicken of 1 age in days, select wheat-dregs of beans daily ration (wheat content 64.53%), add single zytase (group B) respectively, zytase and arabinofuranosidase (group C), zytase and feruloyl esterase (group D), and embodiment 1 compound enzymic preparation (group E), not add the above-mentioned wheat-dregs of beans daily ration of any enzyme for blank (group A), carry out broiler feeding experiment, test and proceed to 36 ages in days by 1 age in days.Experimental design in table 5, the unit of activity of this zymin of data representation in every kg wheat-dregs of beans daily ration in each zymin bracket in table 5.Experimental result is in Table 6-8.
The different zymin of table 5 is formed and consumption
Test observation determines chicken group production performance, chyme stickiness and pH value, intestinal tract injury scoring.
The different zymin of table 6 is on the impact of meat chicken production performance
ADG: average daily gain; ADFI: average daily ingestion amount; FCR: feed-weight ratio; Note: the different letter of shoulder mark represents significant difference (P<0.05)
Result shows, compared with control group, add the production performance that zytase significantly improves broiler chicken, make the day weight gain of 21d broiler chicken and feed-weight ratio bring up to 32.43g from 30.99g, feed-weight ratio reduces to 1.47 from 1.52; Make 36d broiler chicken day weight gain and feed-weight ratio bring up to 51.24g from 48.97g, feed-weight ratio reduces to 1.88 from 1.97.The interpolation of arabinofuranosidase and feruloyl esterase further improves the production performance of broiler chicken, and best with the effect of adding three kinds of enzymes simultaneously.Compared with xylanase (treatments B), the meat chicken production performance of the prozyme (process E) of three kinds of enzyme compositions is not only significantly better than control group, equally higher than treatments B group.The day weight gain of E group 21d and 36d broiler chicken and feed-weight ratio comparatively B component be you can well imagine high and are reduced 6.23%, 5.92%; 12.40%, 10.15%.
Table 7 zymin is on the impact of 36d Intestine of Broiler chyme and viscosity
Note: the different letter of shoulder mark represents significant difference (P<0.05)
Table 8 zymin is on the impact of 36d Intestine of Broiler Injury score
Note: the different letter of shoulder mark represents significant difference (P<0.05)
The interpolation of zytase significantly can reduce broiler chicken Jejunal chyme stickiness, and the interpolation of arabinofuranosidase can reduce chyme viscosity further, and still with additive effect best (table 7) while three kinds of zymins.Now, the Jejunal chyme stickiness of process E group comparatively control group is compared with treatments B, declines 30.87%, 20.16% respectively.Compared with control group, arabinofuranosidase and feruloyl esterase and zytase compatibility use, and significantly can reduce broiler chicken caecum pH value, best with the additive effect of three kinds of zymins equally.In addition, the interpolation of zytase can also reduce the intestinal tract injury scoring of broiler chicken, and the interpolation of arabinofuranosidase and feruloyl esterase can reduce intestinal tract injury scoring further, and best with additive effect while three kinds of zymins.
Although above the present invention is described in detail with a general description of the specific embodiments, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, all belong to the scope of protection of present invention.
Claims (10)
1. a Wheat Based Diet specificity xylan degrading compound enzymic preparation, is characterized in that, its activeconstituents comprises zytase, also comprises arabinofuranosidase and/or feruloyl esterase.
2. compound enzymic preparation according to claim 1, is characterized in that, its activeconstituents comprises zytase 11250-15000U/g, also comprises arabinofuranosidase 2080-3120U/g and/or feruloyl esterase 160-240U/g;
Preferably, its activeconstituents comprises zytase 12000-13750U/g, also comprises arabinofuranosidase 2340-2860U/g and/or feruloyl esterase 180-220U/g.
3. compound enzymic preparation according to claim 2, is characterized in that, its activeconstituents comprises zytase 12500U/g, also comprises arabinofuranosidase 2600U/g and/or feruloyl esterase 200U/g.
4. the compound enzymic preparation according to any one of claim 1-3, is characterized in that, also comprises thinner; Preferably, described thinner is flour and/or defatted rice bran.
5. the compound enzymic preparation according to any one of claim 1-3, is characterized in that, described zytase is β-Isosorbide-5-Nitrae-endo-xylanase.
6. the compound enzymic preparation according to any one of claim 1-3, it is characterized in that, described zytase derives from Rumen Fungi (Neocallimastixpatriciarum), arabinofuranosidase derives from bacillus pumilus (Bacilluspumilus), feruloyl esterase derives from Clostridium thermocellum (Clostridiumthermocellum).
7. the compound enzymic preparation according to any one of claim 1-3, is characterized in that, the enzyme activity unit of described zytase, arabinofuranosidase, feruloyl esterase is respectively 100000U/g, 4000U/g, 3200U/g.
8. the preparation method of compound enzymic preparation described in any one of claim 1-7, is characterized in that, comprise the following steps: get various enzyme as activeconstituents by proportioning, and/or add thinner, mix and get final product.
9. the application of compound enzymic preparation in Wheat Based Diet feed formulation described in any one of claim 1-7.
10. application according to claim 9, is characterized in that, the addition of described compound enzymic preparation in Wheat Based Diet feed is 100-200g/ ton; Preferably, when in Wheat Based Diet feed, wheat weighs less than 30%, described compound enzymic preparation addition is 100g/ ton; When Wheat Based Diet feed wheat weight is more than 30%, described compound enzymic preparation addition is 200g/ ton.
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| CN107637759A (en) * | 2017-10-31 | 2018-01-30 | 南京师范大学 | The wheat bran flour and wheat bran steamed bun of application and its preparation of the arabinosidase in wheat-bran dietary fiber is prepared |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107287175A (en) * | 2017-08-15 | 2017-10-24 | 青岛澳蓝明东生物科技有限公司 | A kind of complex enzyme formulation and its application for xylose production |
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| CN107637759A (en) * | 2017-10-31 | 2018-01-30 | 南京师范大学 | The wheat bran flour and wheat bran steamed bun of application and its preparation of the arabinosidase in wheat-bran dietary fiber is prepared |
| CN109679934A (en) * | 2019-01-07 | 2019-04-26 | 济南百斯杰生物工程有限公司 | A kind of complex enzyme composition of efficient degradation corn xylan |
| CN109679934B (en) * | 2019-01-07 | 2022-06-28 | 济南百斯杰生物工程有限公司 | Complex enzyme composition for degrading corn xylan |
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