CN105459243A - Processing method of cedarwood furniture panels with white rot bacterial plaque - Google Patents
Processing method of cedarwood furniture panels with white rot bacterial plaque Download PDFInfo
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- CN105459243A CN105459243A CN201610007430.5A CN201610007430A CN105459243A CN 105459243 A CN105459243 A CN 105459243A CN 201610007430 A CN201610007430 A CN 201610007430A CN 105459243 A CN105459243 A CN 105459243A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B27—WORKING OR PRESERVING WOOD OR SIMILAR MATERIAL; NAILING OR STAPLING MACHINES IN GENERAL
- B27M—WORKING OF WOOD NOT PROVIDED FOR IN SUBCLASSES B27B - B27L; MANUFACTURE OF SPECIFIC WOODEN ARTICLES
- B27M3/00—Manufacture or reconditioning of specific semi-finished or finished articles
- B27M3/18—Manufacture or reconditioning of specific semi-finished or finished articles of furniture or of doors
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B27—WORKING OR PRESERVING WOOD OR SIMILAR MATERIAL; NAILING OR STAPLING MACHINES IN GENERAL
- B27K—PROCESSES, APPARATUS OR SELECTION OF SUBSTANCES FOR IMPREGNATING, STAINING, DYEING, BLEACHING OF WOOD OR SIMILAR MATERIALS, OR TREATING OF WOOD OR SIMILAR MATERIALS WITH PERMEANT LIQUIDS, NOT OTHERWISE PROVIDED FOR; CHEMICAL OR PHYSICAL TREATMENT OF CORK, CANE, REED, STRAW OR SIMILAR MATERIALS
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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Abstract
The invention relates to a processing method of cedarwood furniture panels with white rot bacterial plaque. The method comprises the following steps: first, carrying out sterilization and humidification treatment on outsourcing cedarwood ecological panels by adopting high temperature steam, and killing other infectious microbes possibly carried by the outsourcing cedarwood ecological panels to prevent the phenomenon of infectious microbe infection; secondly, preparing a white rot fungi culture solution and finally preparing a white rot fungi bacterial solution; then spraying the white rot fungi bacterial solution to the surface of the cedarwood ecological panels painted with culture medium by using a sprayer, and sending to a constant-temperature constant-humidity chamber for cultivation of the white rot bacterial plaque; and finally, sending the cedarwood ecological panels with surfaces covered by the white rot bacterial plaque to a wood drier for drying, so as to produce a furniture panel material with unique decorative effect. The processing technology is simple and feasible, and the unique cedarwood furniture panels with white rot bacterial plaque can be produced by special technical processing by utilizing cedarwood panels with low cost. The technology can meet the requirement of distressing of furniture and antiquing home decoration materials in home decoration.
Description
Technical field
The invention belongs to wood processing field, be specifically related to a kind of processing method with white rot bacterial plaque China fir Furniture panel.
Background technology
Missing old times or old friends is a kind of feelings, when the life of people more tends to steadily, more cherishes the memory of the time that those pass.People often recall the past memories and wake some fine memories up, and the feelings of nature, back to nature are yearned in release.As popular several years ago, jeans are deliberately done old worn-off, nowadays furniture also sweeps " doing old wind ".Simple and unsophisticated furniture, elegant moulding, one " doing old " wind advocating the ancient rhythm of ancient customs is just popular.Timeworn furniture have more vicissitudes sense because the time is remote, meet modern trend, be subject to white collar and middle product consumer pursues, although furniture surface is outmoded, expensive.
Pseudo-classic style of missing old times or old friends praised highly especially by the furniture of American-European countries.Along with the development of China's furniture industry, the furniture exporting America and Europe account for most of furniture total amount.The antique imitation process meeting American-European customer demand has become the prevailing technology of furniture surface process and application, this antique-imitation technique is along with the reinforcement of international exchange, also accept by domestic a part of consumer group, simultaneously at the classic furniture of Chinese Style, with the covering with paint of antique traditional furniture, antique-imitation technique also has certain applications.
The fir wood plank that this processing and utilization is cheap, through special process process, produce the China fir Furniture panel of unique band white rot bacterial plaque, this technology can meet fitment material for family modelled after an antique in furniture and house decoration and make old needs, significantly promote the economic worth of fir wood plank, therefore there is vast potential for future development.
Summary of the invention
The object of the present invention is to provide a kind of processing method with white rot bacterial plaque China fir Furniture panel.The method utilizes cheap fir wood plank, through special process process, produce the China fir Furniture panel of unique band white rot bacterial plaque, this technology can meet fitment material for family modelled after an antique in furniture and house decoration and make old needs, significantly promotes the economic worth of fir wood plank.
The invention provides a kind of processing method with white rot bacterial plaque China fir Furniture panel, the method comprises the steps:
(1) outsourcing specification China fir ecological sheet material (2440mm × 1220mm × 18mm) (long × wide × thick), sends into lumber kiln and carries out high-temp steam sterilizing and add wet process, for subsequent use;
(2) get 24 g potato dextrose culture-mediums (PDb) and 15 ~ 20 grams of agar (Agar) load in 2000 milliliters of vials, add 1000 ml distilled waters, after stirring, put into high-temperature sterilization box and carry out sterilization treatment; Take out after 1 ~ 2 hour, pour in blank, cool after 5 ~ 15 minutes, be after solid shape until nutrient solution, cover cover plate and put into incubator, for subsequent use;
(3) check the situation of solid medium in incubator blank every day, as found, the culture medium in blank has miscellaneous bacteria to infect, and picks immediately and abandons;
(4) whiterot fungi bacterial classification provided by the raw ring of the Chinese Academy of Forestry, utilization connects acicula and connects bacterium to the culture medium in blank respectively, complete after connecing bacterium work and blank is put into incubator, every day takes out the growing state observing whiterot fungi, and the blank finding that there is miscellaneous bacteria infection must pick and abandon;
(5) until the mould bacterial plaque area of whiterot fungi develop into account for whole blank culture medium area 60 ~ 80% after, take out whiterot fungi blank, pour stainless steel pulverizer into and pulverize.In whiterot fungi: distilled water=1:100(mass ratio) ratio add distilled water, modulation whiterot fungi bacterium liquid, mixes well in the reagent bottle of the manual pesticide sprayer of rear loading, for subsequent use;
(6) by potato dextrose medium: agar: distilled water=24:18:1000 (mass ratio) prepares nutrient solution, send in high-temperature sterilization box after loading container and carry out sterilization treatment, take out after 1.5 hours, brush through high-temp steam sterilizing and add on the China fir ecological board of wet process equably with hairbrush;
(7) the China fir ecological sheet material of completing steps 6 is placed on table top, by China fir ecological sheet material area (m
2): whiterot fungi bacterium liquid is evenly sprayed on China fir ecological sheet material with manual pesticide sprayer by the ratio of whiterot fungi bacterium liquid (g)=1:1 ~ 2, after completing sprinkling, China fir ecological sheet material is sent between constant temperature and humidity, lie in a horizontal plane in (spraying area upward) on homemade metal level frame, every interlayer of metal level frame is divided into 8cm, place a China fir ecological sheet material for every layer, metal level frame extreme lower position is apart from the height 10-15cm on ground;
(8) situation of growing of 3 China fir ecological sheet material surface whiterot fungis is observed weekly, as found China fir ecological sheet material surface moisture overdrying, the relative humidity between adjustable thermostatic constant humidity;
(9) after the whiterot fungi growth area on China fir ecological sheet material surface to be found exceedes more than 80% of China fir ecological board surface area, China fir ecological sheet material is taken out between constant temperature and humidity, send into lumber kiln and carry out drying process, baking temperature 80 ~ 100 DEG C, 24 ~ 48 hours drying times, dried moisture control, 8 ~ 10%, makes band white rot bacterial plaque China fir Furniture panel.
In described step 1, outsourcing specification China fir ecological sheet material sends into the object that lumber kiln carries out high-temp steam sterilizing process is thoroughly kill other miscellaneous bacteria that outsourcing fir wood plank may carry, and prevents from occurring the phenomenon that miscellaneous bacteria infects in the process of cultivating whiterot fungi.The temperature of steam high-temperature sterilization controls at 120 DEG C, and the processing time controlled at 20 ~ 30 minutes.
In described step 1, outsourcing specification China fir ecological sheet material feeding lumber kiln carries out adding the object of wet process is that the moisture content of outsourcing China fir ecological sheet material is brought up to 30 ~ 60% from 10%, and the normal growth being conducive to later stage whiterot fungi is grown.
After completing the preparation of nutrient solution in described step 2, blank must send in incubator the incubation of carrying out 3 days by a definite date, and the temperature of incubator controls at 25 ± 2 DEG C, and relative humidity controls 85 ~ 90%.
Utilize in described step 4 and connect acicula respectively to the process of the culture medium inoculated whiterot fungi in blank, in order to prevent the infection of miscellaneous bacteria, whole seeded process must complete in aseptic operating room, the exhaust fan must opening fume hood ventilates, must with 1% alcohol liquid to workbench pasteurised completely, connecing in bacterium process, connecing acicula must sterilize with alcolhol burner calcination.
In described step 4, every day observes whiterot fungi growing state, in order to prevent the infection of miscellaneous bacteria, must not open blank and observing whiterot fungi growing state.
Prepare in the process of whiterot fungi bacterium liquid in described step 5, the stainless steel pulverizer pulverized for whiterot fungi has to pass through alcohol disinfecting before use, prepare whiterot fungi bacterium liquid distilled water used must ensure, definitely without mycotic infection, also to have to pass through alcohol disinfecting before use for the pesticide sprayer spraying whiterot fungi bacterium liquid.
In described step 6 preparation and brushing nutrient solution process in, the consumption of nutrient solution must ensure to be used while allocating, prevent from causing brushing not as good as forming gel because preparing too much nutrient solution, produce unnecessary loss, fir wood plank one side nutrient solution brushing amount controls: 260 ~ 300g/m
2.
Complete one side in described step 7 to spray between the China fir ecological sheet material feeding constant temperature and humidity of whiterot fungi bacterium liquid, the temperature between constant temperature and humidity controls at 25 ± 2 DEG C, and relative humidity controls 85 ~ 90%; The height placing the metal frame extreme lower position distance ground of China fir ecological board is greater than 10cm, prevents other miscellaneous bacterias ground from infecting.
After finding in described step 9 that the whiterot fungi growth area on China fir ecological sheet material surface exceedes more than 80% of China fir ecological board surface area; China fir ecological board is sent into lumber kiln and carry out drying process; for protecting the neat of the white rot bacterial plaque of China fir ecological board surface formation and integrality, notice that in dry run, China fir ecological board must be contained in the interior drying separately of independently metallic spacer layer.
The invention has the advantages that: a kind of processing method with white rot bacterial plaque China fir Furniture panel is provided.The method utilizes cheap fir wood plank, through special process process, produce the China fir Furniture panel of unique band white rot bacterial plaque, this technology can meet fitment material for family modelled after an antique in furniture and house decoration and make old needs, significantly promotes the economic worth of fir wood plank.
Detailed description of the invention
Embodiment 1
A kind of concrete steps of the processing method with white rot bacterial plaque China fir Furniture panel are:
Step 1: outsourcing specification China fir ecological sheet material (2440mm × 1220mm × 18mm) (long × wide × thick), sends into lumber kiln and carries out high-temp steam sterilizing and add wet process, for subsequent use;
Step 2: get 24 g potato dextrose culture-mediums (PDb) and 15 grams of agar (Agar) load in 2000 milliliters of vials, adds 1000 ml distilled waters, after stirring, puts into high-temperature sterilization box and carry out sterilization treatment; Take out after 1 hour, pour in blank, cool after 5 minutes, be after solid shape until nutrient solution, cover cover plate and put into incubator, for subsequent use;
Step 3: the situation checking solid medium in incubator blank every day, as found, the culture medium in blank has miscellaneous bacteria to infect, and picks immediately and abandons;
Step 4: whiterot fungi bacterial classification provided by the raw ring of the Chinese Academy of Forestry, utilization connects acicula and connects bacterium to the culture medium in blank respectively, complete after connecing bacterium work and blank is put into incubator, every day takes out the growing state observing whiterot fungi, and the blank finding that there is miscellaneous bacteria infection must pick and abandon;
Step 5: until the mould bacterial plaque area of whiterot fungi develop into account for whole blank culture medium area 60% after, take out whiterot fungi blank, pour stainless steel pulverizer into and pulverize.In whiterot fungi: distilled water=1:100(mass ratio) ratio add distilled water, modulation whiterot fungi bacterium liquid, mixes well in the reagent bottle of the manual pesticide sprayer of rear loading, for subsequent use;
Step 6: by potato dextrose medium: agar: distilled water=24:18:1000 (mass ratio) prepares nutrient solution, send in high-temperature sterilization box after loading container and carry out sterilization treatment, take out after 1.5 hours, brush through high-temp steam sterilizing and add on the China fir ecological board of wet process equably with hairbrush;
Step 7: be placed on table top by the China fir ecological sheet material of completing steps 6, by China fir ecological sheet material area (m
2): whiterot fungi bacterium liquid is evenly sprayed on China fir ecological sheet material with manual pesticide sprayer by the ratio of whiterot fungi bacterium liquid (g)=1:1, after completing sprinkling, China fir ecological sheet material is sent between constant temperature and humidity, lie in a horizontal plane in (spraying area upward) on homemade metal level frame, every interlayer of metal level frame is divided into 8cm, place a China fir ecological sheet material for every layer, metal level frame extreme lower position is apart from the height 10cm on ground;
Step 8: the situation of growing of observing weekly 3 China fir ecological sheet material surface whiterot fungis, as found China fir ecological sheet material surface moisture overdrying, the relative humidity between adjustable thermostatic constant humidity;
Step 9: the whiterot fungi growth on China fir ecological sheet material surface to be found is after area exceedes more than 80% of China fir ecological board surface area, China fir ecological sheet material is taken out between constant temperature and humidity, send into lumber kiln and carry out drying process, baking temperature 80 DEG C, 48 hours drying times, dried moisture control, 8%, makes band white rot bacterial plaque China fir Furniture panel.
In described step 1, outsourcing specification China fir ecological sheet material sends into the object that lumber kiln carries out high-temp steam sterilizing process is thoroughly kill other miscellaneous bacteria that outsourcing fir wood plank may carry, and prevents from occurring the phenomenon that miscellaneous bacteria infects in the process of cultivating whiterot fungi.The temperature of steam high-temperature sterilization controls at 120 DEG C, and the processing time controlled at 20 minutes.
In described step 1, outsourcing specification China fir ecological sheet material feeding lumber kiln carries out adding the object of wet process is that the moisture content of outsourcing China fir ecological sheet material is brought up to 30 ~ 60% from 10%, and the normal growth being conducive to later stage whiterot fungi is grown.
After completing the preparation of nutrient solution in described step 2, blank must send in incubator the incubation of carrying out 3 days by a definite date, and the temperature of incubator controls at 25 ± 2 DEG C, and relative humidity controls 85%.
Utilize in described step 4 and connect acicula respectively to the process of the culture medium inoculated whiterot fungi in blank, in order to prevent the infection of miscellaneous bacteria, whole seeded process must complete in aseptic operating room, the exhaust fan must opening fume hood ventilates, must with 1% alcohol liquid to workbench pasteurised completely, connecing in bacterium process, connecing acicula must sterilize with alcolhol burner calcination.
In described step 4, every day observes whiterot fungi growing state, in order to prevent the infection of miscellaneous bacteria, must not open blank and observing whiterot fungi growing state.
Prepare in the process of whiterot fungi bacterium liquid in described step 5, the stainless steel pulverizer pulverized for whiterot fungi has to pass through alcohol disinfecting before use, prepare whiterot fungi bacterium liquid distilled water used must ensure, definitely without mycotic infection, also to have to pass through alcohol disinfecting before use for the pesticide sprayer spraying whiterot fungi bacterium liquid.
In described step 6 preparation and brushing nutrient solution process in, the consumption of nutrient solution must ensure to be used while allocating, prevent from causing brushing not as good as forming gel because preparing too much nutrient solution, produce unnecessary loss, fir wood plank one side nutrient solution brushing amount controls: 260g/m
2.
Complete one side in described step 7 to spray between the China fir ecological sheet material feeding constant temperature and humidity of whiterot fungi bacterium liquid, the temperature between constant temperature and humidity controls at 25 ± 2 DEG C, and relative humidity controls 85%; Place the height 10cm of metal frame extreme lower position apart from ground of China fir ecological board, prevent other miscellaneous bacterias ground from infecting.
After finding in described step 9 that the whiterot fungi growth area on China fir ecological sheet material surface exceedes more than 80% of China fir ecological board surface area; China fir ecological board is sent into lumber kiln and carry out drying process; for protecting the neat of the white rot bacterial plaque of China fir ecological board surface formation and integrality, notice that in dry run, China fir ecological board must be contained in the interior drying separately of independently metallic spacer layer.
Embodiment 2
A kind of concrete steps of the processing method with white rot bacterial plaque China fir Furniture panel are:
Step 1: outsourcing specification China fir ecological sheet material (2440mm × 1220mm × 18mm) (long × wide × thick), sends into lumber kiln and carries out high-temp steam sterilizing and add wet process, for subsequent use;
Step 2: get 24 g potato dextrose culture-mediums (PDb) and 20 grams of agar (Agar) load in 2000 milliliters of vials, adds 1000 ml distilled waters, after stirring, puts into high-temperature sterilization box and carry out sterilization treatment; Take out after 2 hours, pour in blank, cool after 15 minutes, be after solid shape until nutrient solution, cover cover plate and put into incubator, for subsequent use;
Step 3: the situation checking solid medium in incubator blank every day, as found, the culture medium in blank has miscellaneous bacteria to infect, and picks immediately and abandons;
Step 4: whiterot fungi bacterial classification provided by the raw ring of the Chinese Academy of Forestry, utilization connects acicula and connects bacterium to the culture medium in blank respectively, complete after connecing bacterium work and blank is put into incubator, every day takes out the growing state observing whiterot fungi, and the blank finding that there is miscellaneous bacteria infection must pick and abandon;
Step 5: until the mould bacterial plaque area of whiterot fungi develop into account for whole blank culture medium area 80% after, take out whiterot fungi blank, pour stainless steel pulverizer into and pulverize.In whiterot fungi: distilled water=1:100(mass ratio) ratio add distilled water, modulation whiterot fungi bacterium liquid, mixes well in the reagent bottle of the manual pesticide sprayer of rear loading, for subsequent use;
Step 6: by potato dextrose medium: agar: distilled water=24:18:1000 (mass ratio) prepares nutrient solution, send in high-temperature sterilization box after loading container and carry out sterilization treatment, take out after 1.5 hours, brush through high-temp steam sterilizing and add on the China fir ecological board of wet process equably with hairbrush;
Step 7: be placed on table top by the China fir ecological sheet material of completing steps 6, by China fir ecological sheet material area (m
2): whiterot fungi bacterium liquid is evenly sprayed on China fir ecological sheet material with manual pesticide sprayer by the ratio of whiterot fungi bacterium liquid (g)=1:2, after completing sprinkling, China fir ecological sheet material is sent between constant temperature and humidity, lie in a horizontal plane in (spraying area upward) on homemade metal level frame, every interlayer of metal level frame is divided into 8cm, place a China fir ecological sheet material for every layer, metal level frame extreme lower position is apart from the height 15cm on ground;
Step 8: the situation of growing of observing weekly 3 China fir ecological sheet material surface whiterot fungis, as found China fir ecological sheet material surface moisture overdrying, the relative humidity between adjustable thermostatic constant humidity;
Step 9: the whiterot fungi growth on China fir ecological sheet material surface to be found is after area exceedes more than 80% of China fir ecological board surface area, China fir ecological sheet material is taken out between constant temperature and humidity, send into lumber kiln and carry out drying process, baking temperature 100 DEG C, 24 hours drying times, dried moisture control, 10%, makes band white rot bacterial plaque China fir Furniture panel.
Preferably, in described step 1, outsourcing specification China fir ecological sheet material sends into the object that lumber kiln carries out high-temp steam sterilizing process is thoroughly kill other miscellaneous bacteria that outsourcing fir wood plank may carry, and prevents from occurring the phenomenon that miscellaneous bacteria infects in the process of cultivating whiterot fungi.The temperature of steam high-temperature sterilization controls at 120 DEG C, and the processing time controlled at 30 minutes.
Preferably, in described step 1, outsourcing specification China fir ecological sheet material feeding lumber kiln carries out adding the object of wet process is that the moisture content of outsourcing China fir ecological sheet material is brought up to 60% from 10%, and the normal growth being conducive to later stage whiterot fungi is grown.
Preferably, after completing the preparation of nutrient solution in described step 2, blank must send in incubator the incubation of carrying out 3 days by a definite date, and the temperature of incubator controls at 25 ± 2 DEG C, and relative humidity controls 90%.
Preferably, utilize in described step 4 and connect acicula respectively to the process of the culture medium inoculated whiterot fungi in blank, in order to prevent the infection of miscellaneous bacteria, whole seeded process must complete in aseptic operating room, the exhaust fan must opening fume hood ventilates, must with 1% alcohol liquid to workbench pasteurised completely, connecing in bacterium process, connect acicula must with alcolhol burner calcination sterilization.
Preferably, in described step 4, every day observes whiterot fungi growing state, in order to prevent the infection of miscellaneous bacteria, must not open blank and observing whiterot fungi growing state.
Preferably, prepare in the process of whiterot fungi bacterium liquid in described step 5, the stainless steel pulverizer pulverized for whiterot fungi has to pass through alcohol disinfecting before use, prepare whiterot fungi bacterium liquid distilled water used must ensure, definitely without mycotic infection, also to have to pass through alcohol disinfecting before use for the pesticide sprayer spraying whiterot fungi bacterium liquid.
Preferably, in described step 6 preparation and brushing nutrient solution process in, the consumption of nutrient solution must ensure to be used while allocating, prevent because of prepare too much nutrient solution cause brushing not as good as formed gel, produce unnecessary loss, fir wood plank one side nutrient solution brushing amount controls: 300g/m
2.
Preferably, complete one side in described step 7 and spray between the China fir ecological sheet material feeding constant temperature and humidity of whiterot fungi bacterium liquid, the temperature between constant temperature and humidity controls at 25 ± 2 DEG C, and relative humidity controls 90%; Place the height 15cm of metal frame extreme lower position apart from ground of China fir ecological board, prevent other miscellaneous bacterias ground from infecting.
Preferably; after finding in described step 9 that the whiterot fungi growth area on China fir ecological sheet material surface exceedes more than 80% of China fir ecological board surface area; China fir ecological board is sent into lumber kiln and carry out drying process; for protecting the neat of the white rot bacterial plaque of China fir ecological board surface formation and integrality, notice that in dry run, China fir ecological board must be contained in the interior drying separately of independently metallic spacer layer.
Embodiment 3
A kind of concrete steps of the processing method with white rot bacterial plaque China fir Furniture panel are:
Step 1: outsourcing specification China fir ecological sheet material (2440mm × 1220mm × 18mm) (long × wide × thick), sends into lumber kiln and carries out high-temp steam sterilizing and add wet process, for subsequent use;
Step 2: get 24 g potato dextrose culture-mediums (PDb) and 17 grams of agar (Agar) load in 2000 milliliters of vials, adds 1000 ml distilled waters, after stirring, puts into high-temperature sterilization box and carry out sterilization treatment; Take out after 1.5 hours, pour in blank, cool after 10 minutes, be after solid shape until nutrient solution, cover cover plate and put into incubator, for subsequent use;
Step 3: the situation checking solid medium in incubator blank every day, as found, the culture medium in blank has miscellaneous bacteria to infect, and picks immediately and abandons;
Step 4: whiterot fungi bacterial classification provided by the raw ring of the Chinese Academy of Forestry, utilization connects acicula and connects bacterium to the culture medium in blank respectively, complete after connecing bacterium work and blank is put into incubator, every day takes out the growing state observing whiterot fungi, and the blank finding that there is miscellaneous bacteria infection must pick and abandon;
Step 5: until the mould bacterial plaque area of whiterot fungi develop into account for whole blank culture medium area 80% after, take out whiterot fungi blank, pour stainless steel pulverizer into and pulverize.In whiterot fungi: distilled water=1:100(mass ratio) ratio add distilled water, modulation whiterot fungi bacterium liquid, mixes well in the reagent bottle of the manual pesticide sprayer of rear loading, for subsequent use;
Step 6: by potato dextrose medium: agar: distilled water=24:18:1000 (mass ratio) prepares nutrient solution, send in high-temperature sterilization box after loading container and carry out sterilization treatment, take out after 1.5 hours, brush through high-temp steam sterilizing and add on the China fir ecological board of wet process equably with hairbrush;
Step 7: be placed on table top by the China fir ecological sheet material of completing steps 6, by China fir ecological sheet material area (m
2): whiterot fungi bacterium liquid is evenly sprayed on China fir ecological sheet material with manual pesticide sprayer by the ratio of whiterot fungi bacterium liquid (g)=1:2, after completing sprinkling, China fir ecological sheet material is sent between constant temperature and humidity, lie in a horizontal plane in (spraying area upward) on homemade metal level frame, every interlayer of metal level frame is divided into 8cm, place a China fir ecological sheet material for every layer, metal level frame extreme lower position is apart from the height 10cm on ground;
Step 8: the situation of growing of observing weekly 3 China fir ecological sheet material surface whiterot fungis, as found China fir ecological sheet material surface moisture overdrying, the relative humidity between adjustable thermostatic constant humidity;
Step 9: the whiterot fungi growth on China fir ecological sheet material surface to be found is after area exceedes more than 90% of China fir ecological board surface area, China fir ecological sheet material is taken out between constant temperature and humidity, send into lumber kiln and carry out drying process, baking temperature 90 DEG C, 36 hours drying times, dried moisture control, 9%, makes band white rot bacterial plaque China fir Furniture panel.
Preferably, in described step 1, outsourcing specification China fir ecological sheet material sends into the object that lumber kiln carries out high-temp steam sterilizing process is thoroughly kill other miscellaneous bacteria that outsourcing fir wood plank may carry, and prevents from occurring the phenomenon that miscellaneous bacteria infects in the process of cultivating whiterot fungi.The temperature of steam high-temperature sterilization controls at 120 DEG C, and the processing time controlled at 25 minutes.
Preferably, in described step 1, outsourcing specification China fir ecological sheet material feeding lumber kiln carries out adding the object of wet process is that the moisture content of outsourcing China fir ecological sheet material is brought up to 50% from 10%, and the normal growth being conducive to later stage whiterot fungi is grown.
Preferably, after completing the preparation of nutrient solution in described step 2, blank must send in incubator the incubation of carrying out 3 days by a definite date, and the temperature of incubator controls at 25 ± 2 DEG C, and relative humidity controls 90%.
Preferably, utilize in described step 4 and connect acicula respectively to the process of the culture medium inoculated whiterot fungi in blank, in order to prevent the infection of miscellaneous bacteria, whole seeded process must complete in aseptic operating room, the exhaust fan must opening fume hood ventilates, must with 1% alcohol liquid to workbench pasteurised completely, connecing in bacterium process, connect acicula must with alcolhol burner calcination sterilization.
Preferably, in described step 4, every day observes whiterot fungi growing state, in order to prevent the infection of miscellaneous bacteria, must not open blank and observing whiterot fungi growing state.
Preferably, prepare in the process of whiterot fungi bacterium liquid in described step 5, the stainless steel pulverizer pulverized for whiterot fungi has to pass through alcohol disinfecting before use, prepare whiterot fungi bacterium liquid distilled water used must ensure, definitely without mycotic infection, also to have to pass through alcohol disinfecting before use for the pesticide sprayer spraying whiterot fungi bacterium liquid.
Preferably, in described step 6 preparation and brushing nutrient solution process in, the consumption of nutrient solution must ensure to be used while allocating, prevent because of prepare too much nutrient solution cause brushing not as good as formed gel, produce unnecessary loss, fir wood plank one side nutrient solution brushing amount controls: 300g/m
2.
Preferably, complete one side in described step 7 and spray between the China fir ecological sheet material feeding constant temperature and humidity of whiterot fungi bacterium liquid, the temperature between constant temperature and humidity controls at 25 ± 2 DEG C, and relative humidity controls 90%; Place the height 10cm of metal frame extreme lower position apart from ground of China fir ecological board, prevent other miscellaneous bacterias ground from infecting.
Preferably; after finding in described step 9 that the whiterot fungi growth area on China fir ecological sheet material surface exceedes more than 80% of China fir ecological board surface area; China fir ecological board is sent into lumber kiln and carry out drying process; for protecting the neat of the white rot bacterial plaque of China fir ecological board surface formation and integrality, notice that in dry run, China fir ecological board must be contained in the interior drying separately of independently metallic spacer layer.
The band white rot bacterial plaque China fir Furniture panel prepared by the present invention, there is feature simple to operate, easy to learn, the method can utilize cheap fir wood plank, through special process process, produce band white rot bacterial plaque China fir Furniture panel, this technology can meet fitment material for family modelled after an antique in furniture and house decoration and make old needs, significantly promotes the economic worth of fir wood plank, therefore has vast potential for future development.
The foregoing is only preferred embodiment of the present invention, all equalizations done according to the present patent application the scope of the claims change and modify, and all should belong to covering scope of the present invention.
Claims (10)
1. the processing method with white rot bacterial plaque China fir Furniture panel, is characterized in that, comprise the steps:
(1) outsourcing specification China fir ecological sheet material, length × wide × thick=2440mm × 1220mm × 18mm, sends into lumber kiln and carries out high-temp steam sterilizing and add wet process, for subsequent use;
(2) get 24 g potato dextrose culture-mediums and 15 ~ 20 grams of agar load in 2000 milliliters of vials, add 1000 ml distilled waters, after stirring, put into high-temperature sterilization box and carry out sterilization treatment; Take out after 1 ~ 2 hour, pour in blank, cool after 5 ~ 15 minutes, be after solid shape until nutrient solution, cover cover plate and put into incubator, for subsequent use;
(3) check the situation of solid medium in incubator blank every day, as found, the culture medium in blank has miscellaneous bacteria to infect, and picks immediately and abandons;
(4) utilization connects acicula and connects bacterium to the culture medium in blank respectively, and the bacterium of employing is whiterot fungi, and complete after connecing bacterium work and blank is put into incubator, every day takes out the growing state observing whiterot fungi, and the blank finding that there is miscellaneous bacteria infection must pick and abandon;
(5) until the mould bacterial plaque area of whiterot fungi develop into account for whole blank culture medium area 60 ~ 80% after, take out whiterot fungi blank, pour stainless steel pulverizer into pulverize, in whiterot fungi: the ratio of distilled water mass ratio=1:100 adds distilled water, modulation whiterot fungi bacterium liquid, mix well in the reagent bottle of the manual pesticide sprayer of rear loading, for subsequent use;
(6) by potato dextrose medium: agar: distilled water mass ratio=24:18:1000 prepares nutrient solution, send in high-temperature sterilization box after loading container and carry out sterilization treatment, take out after 1.5 hours, brush through high-temp steam sterilizing and add on the China fir ecological board of wet process equably with hairbrush;
(7) the China fir ecological sheet material of completing steps 6 is placed on table top, in China fir ecological sheet material area: whiterot fungi bacterium liquid is evenly sprayed on China fir ecological sheet material with manual pesticide sprayer by the ratio of whiterot fungi bacterium liquid quality=1:1 ~ 2, after completing sprinkling, China fir ecological sheet material is sent between constant temperature and humidity, lie in a horizontal plane on homemade metal level frame, spraying area upward, every interlayer of metal level frame is divided into 8cm, place a China fir ecological sheet material for every layer, metal level frame extreme lower position is apart from the height 10-15cm on ground;
(8) situation of growing of 3 China fir ecological sheet material surface whiterot fungis is observed weekly, as found China fir ecological sheet material surface moisture overdrying, the relative humidity between adjustable thermostatic constant humidity;
(9) after the whiterot fungi growth area on China fir ecological sheet material surface to be found exceedes more than 80% of China fir ecological board surface area, China fir ecological sheet material is taken out between constant temperature and humidity, send into lumber kiln and carry out drying process, baking temperature 80 ~ 100 DEG C, 24 ~ 48 hours drying times, dried moisture control, 8 ~ 10%, makes band white rot bacterial plaque China fir Furniture panel.
2. a kind of processing method with white rot bacterial plaque China fir Furniture panel according to claim 1, is characterized in that: in step 1, the temperature of high-temp steam sterilizing controls at 120 DEG C, and the processing time controlled at 20 ~ 30 minutes.
3. a kind of processing method with white rot bacterial plaque China fir Furniture panel according to claim 1, it is characterized in that: the object that the specification of outsourcing described in step 1 China fir ecological sheet material feeding lumber kiln carries out adding wet process is that the moisture content of outsourcing China fir ecological sheet material is brought up to 30 ~ 60% from 10%, and the normal growth being conducive to later stage whiterot fungi is grown.
4. a kind of processing method with white rot bacterial plaque China fir Furniture panel according to claim 1, it is characterized in that: after completing the preparation of nutrient solution described in step 2, blank must send in incubator the incubation of carrying out 3 days by a definite date, the temperature of incubator controls at 25 ± 2 DEG C, and relative humidity controls 85 ~ 90%.
5. a kind of processing method with white rot bacterial plaque China fir Furniture panel according to claim 1, it is characterized in that: utilize described in step 4 and connect acicula respectively to the process of the culture medium inoculated whiterot fungi in blank, in order to prevent the infection of miscellaneous bacteria, whole seeded process must complete in aseptic operating room, the exhaust fan must opening fume hood ventilates, must with 1% alcohol liquid to workbench pasteurised completely, connecing in bacterium process, connect acicula must with alcolhol burner calcination sterilization.
6. a kind of processing method with white rot bacterial plaque China fir Furniture panel according to claim 1, is characterized in that: every day described in step 4 observes whiterot fungi growing state, in order to prevent the infection of miscellaneous bacteria, must not open blank and observing whiterot fungi growing state.
7. a kind of processing method with white rot bacterial plaque China fir Furniture panel according to claim 1, it is characterized in that: prepare in the process of whiterot fungi bacterium liquid described in step 5, the stainless steel pulverizer pulverized for whiterot fungi has to pass through alcohol disinfecting before use, prepare whiterot fungi bacterium liquid distilled water used must ensure, definitely without mycotic infection, also to have to pass through alcohol disinfecting before use for the pesticide sprayer spraying whiterot fungi bacterium liquid.
8. a kind of processing method with white rot bacterial plaque China fir Furniture panel according to claim 1, it is characterized in that: described in step 6 preparation and brushing nutrient solution process in, the consumption of nutrient solution must ensure to be used while allocating, prevent from causing brushing not as good as forming gel because preparing too much nutrient solution, produce unnecessary loss, fir wood plank one side nutrient solution brushing amount controls: 260 ~ 300g/m
2.
9. a kind of processing method with white rot bacterial plaque China fir Furniture panel according to claim 1, it is characterized in that: complete one side described in step 7 and spray between the China fir ecological sheet material feeding constant temperature and humidity of whiterot fungi bacterium liquid, temperature between constant temperature and humidity controls at 25 ± 2 DEG C, and relative humidity controls 85 ~ 90%; Place the height 10-15cm of metal frame extreme lower position apart from ground of China fir ecological board, prevent other miscellaneous bacterias ground from infecting.
10. a kind of processing method with white rot bacterial plaque China fir Furniture panel according to claim 1; it is characterized in that: after finding described in step 9 that the whiterot fungi growth area on China fir ecological sheet material surface exceedes more than 80% of China fir ecological board surface area; China fir ecological board is sent into lumber kiln and carry out drying process; for protecting the neat of the white rot bacterial plaque of China fir ecological board surface formation and integrality, notice that in dry run, China fir ecological board must be contained in the interior drying separately of independently metallic spacer layer.
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