CN105454091A - Experimental device for researching behavioral characteristics of bee under electrical stimulation - Google Patents
Experimental device for researching behavioral characteristics of bee under electrical stimulation Download PDFInfo
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- CN105454091A CN105454091A CN201610025585.1A CN201610025585A CN105454091A CN 105454091 A CN105454091 A CN 105454091A CN 201610025585 A CN201610025585 A CN 201610025585A CN 105454091 A CN105454091 A CN 105454091A
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- 230000000638 stimulation Effects 0.000 title claims abstract description 39
- 230000003542 behavioural effect Effects 0.000 title claims abstract description 27
- 238000005424 photoluminescence Methods 0.000 claims description 140
- 241000256844 Apis mellifera Species 0.000 claims description 107
- 230000015572 biosynthetic process Effects 0.000 claims description 3
- 238000012546 transfer Methods 0.000 claims description 3
- 230000007704 transition Effects 0.000 claims description 3
- 230000006399 behavior Effects 0.000 abstract description 22
- 238000002474 experimental method Methods 0.000 abstract description 6
- 238000000034 method Methods 0.000 abstract description 6
- 230000008569 process Effects 0.000 abstract description 5
- 230000006378 damage Effects 0.000 abstract description 3
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 9
- 238000011160 research Methods 0.000 description 7
- 229910052802 copper Inorganic materials 0.000 description 6
- 239000010949 copper Substances 0.000 description 6
- 238000010586 diagram Methods 0.000 description 5
- 230000004936 stimulating effect Effects 0.000 description 4
- 238000013461 design Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 238000002203 pretreatment Methods 0.000 description 2
- 241000015607 Apis mellifera carnica Species 0.000 description 1
- 241000040710 Chela Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 230000007087 memory ability Effects 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000001537 neural effect Effects 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K47/00—Beehives
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K47/00—Beehives
- A01K47/06—Other details of beehives, e.g. ventilating devices, entrances to hives, guards, partitions or bee escapes
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The invention provides an experimental device for researching behavioral characteristics of a bee under electrical stimulation. The device comprises a stimulation chamber which is internally provided with an electrical stimulation unit, a bee escape behavior information acquisition unit and a bee sound information acquisition unit, the electrical stimulation unit comprises a first electrical stimulation area and a second electrical stimulation area, on and off between the first electrical stimulation area or the second electrical stimulation area and a power supply are controlled through a main controller, the bee escape behavior information acquisition unit and the bee sound information acquisition unit are both connected with the main controller, and the main controller determines which area is energized according to the current position of the bee. According to the experimental device for researching the behavioral characteristics of the bee under the electrical stimulation, escape behaviors and sound behaviors of the bee under the electrical stimulation are researched, pretreatment does not need to be conducted on a subject bee before the experiment, the procedure is simplified, harm caused by pretreatment to the subject bee is avoided, and the experimental results are more reliable. Automatic control is adopted in the whole experimental process, and the influences of human operation errors and human disturbance on bee behaviors on the experimental results are avoided.
Description
Technical field
The invention belongs to insect studying technological domain, be specifically related to a kind of experimental provision studying honeybee behavioral trait under electro photoluminescence.
Background technology
Honeybee has surprising learning and memory ability, is to zoologize to learn the pattern species with memory neural basal.Electro photoluminescence is applied in research process as a kind of research means, but majority concentrates on the research of electrode implanted, and the research of electrode implanted is larger to honeybee injury.Within 2014, American scientist doctor ArianAvalos has carried out the experiment of honeybee electro photoluminescence, honeybee is placed on by he to be stimulated in chamber, honeybee is placed in closed cavity, in chamber, diapire arranges metal grate, the voltage of varying strength is used to stimulate honeybee, find when voltage exceedes certain threshold value, honeybee can stretch out chela pin, shows attack.Current research shows, honeybee, when being subject to some strength electro photoluminescence, can being fled from fast, and sound.Namely honeybee may show a series of behavior reaction under electro photoluminescence, but not yet has related equipment to study a series of behavioural characteristics of honeybee under electro photoluminescence at present.Given this, with reference to the research honeybee of ArianAvalos design by the experimental provision of attack during electro photoluminescence, invent a kind of new escape behavior of research honeybee under electro photoluminescence and the experimental provision of song behavior.
Summary of the invention
The object of the invention is, in order to solve in prior art the defect lacking the device that the behavioural characteristic that shows under electro photoluminescence honeybee is studied, to provide a kind of experimental provision studying honeybee behavioral trait under electro photoluminescence to solve the problems referred to above.
The present invention is achieved through the following technical solutions above-mentioned technology contents:
Study an experimental provision for honeybee behavioral trait under electro photoluminescence, described device comprises stimulation chamber; Electro photoluminescence unit, honeybee escape behavior information acquisition unit, honeybee acoustic information collecting unit is furnished with in described stimulation chamber; Described electro photoluminescence unit comprises the first electro photoluminescence region and the second electro photoluminescence region; Described first electro photoluminescence region is connected with power supply respectively with the second electro photoluminescence region; Described first electro photoluminescence region or the break-make between the second electro photoluminescence region and described power supply adopt main controller controls; Described honeybee escape behavior information acquisition unit is connected with described master controller; Described honeybee escape behavior information acquisition unit collects honeybee position and is transferred to master controller; Described master controller according to described in the honeybee position that collects determine the power supply in connection first electro photoluminescence region or the second electro photoluminescence region; Described acoustic information collecting unit is connected with described master controller; Described acoustic information collecting unit collects the voice signal of honeybee and is transferred to described master controller.
Preferably, described stimulation chamber has roof and diapire.
Preferably, described first electro photoluminescence region is divided into first to power on stimulated zone and first time electro photoluminescence region; Second electro photoluminescence region is divided into second to power on stimulated zone and second time electro photoluminescence region; Described first stimulated zone and second stimulated zone that powers on that powers on is fixed on inside the roof of described stimulation chamber; Described first time electro photoluminescence region and second time electro photoluminescence region are fixed on the inside bottom wall of described stimulation chamber.
Preferably, described first stimulated zone and the described second stimulated zone area sum that powers on that powers on is area inside described stimulation chamber roof; Described first time electro photoluminescence region and described second time electro photoluminescence region area sum are area inside described stimulation chamber bottom.
Preferably, described first power on stimulated zone, the second time electro photoluminescence region of stimulated zone, first time electro photoluminescence region, second that power on includes positive pole, negative pole; Described positive pole, negative pole are staggered formation lattice-shaped; Described first the power on positive pole of stimulated zone and the positive pole in described first time electro photoluminescence region is electrically connected; Described second the power on positive pole of stimulated zone and the positive pole in described second time electro photoluminescence region is electrically connected.
Preferably, described honeybee escape behavior information acquisition unit comprises multiple infrared transmitting tube and the multiple infrared receiving tubes corresponding with described multiple infrared transmitting tube; Described multiple infrared transmitting tube is fixed on the same side in described first electro photoluminescence region and the second electro photoluminescence region; Described multiple infrared receiving tube is separately fixed at the opposite side in described first electro photoluminescence region and the second electro photoluminescence region, and described multiple infrared transmitting tube and described multiple infrared receiving tube are symmetrical arranged.
Preferably, the symmetric points line of described multiple infrared transmitting tube and multiple infrared receiving tube runs through described first electro photoluminescence region and the second electro photoluminescence region.
Preferably, described honeybee acoustic information collecting unit comprises microphone and filter circuit; Described microphone is fixed in described stimulation chamber; Described microphone changes voice signal into the signal of telecommunication and is transferred to filter circuit; Filtered electrical signal transfer is given described master controller by described filter circuit; Analog-signal transitions is data signal by described master controller.
Preferably, described master controller is connected with computer; The honeybee positional information received and acoustic information are transferred to computer by described master controller.
The present invention compared with prior art, has following beneficial effect:
Experimental provision of the present invention, does not need on pretreatment to carry out any pre-treatment to tested honeybee, substantially reduces the Preparatory work of experiment time, and avoid pre-treatment to damage to tested honeybee.The honeybee of free state freely can creep at stimulation chamber simultaneously, and the honeybee as far as possible in maintenance experimentation in the raw, avoids the impact of human factor, improves the reliability of experimental result.
Experimental provision of the present invention, adopts infrared tube and Electret condenser microphone to gather position and the acoustic information of honeybee, can determine the escape behavior of quantifier elimination honeybee under different electro photoluminescence and song behavior.
Experimental provision of the present invention, uses infrared tube to gather position instead of the photographed images collection of honeybee, device can be worked under dark surrounds, and reduce the workload of subsequent result analysis.
Experimental provision of the present invention, takes automatic control at whole experimentation, and structure is simple, easy to operate, avoids manual operation error and the behavior of artificial disturbance honeybee on the impact of experimental result.
Accompanying drawing explanation
Fig. 1 is a kind of perspective view studying honeybee experimental provision of behavioral trait under electro photoluminescence of the present invention;
Fig. 2 is a kind of stereochemical structure partition schematic diagram studying honeybee experimental provision of behavioral trait under electro photoluminescence of the present invention;
Fig. 3 is a kind of copper grid map studying the experimental provision of honeybee behavioral trait under electro photoluminescence of the present invention;
Fig. 4 is the stimulation chamber structure schematic diagram that infrared transmitting tube side is housed after a kind of experimental provision studying honeybee behavioral trait under electro photoluminescence of the present invention removes copper grid and roof;
Fig. 5 is the stimulation chamber structure schematic diagram that infrared receiving tube side is housed after a kind of experimental provision studying honeybee behavioral trait under electro photoluminescence of the present invention removes copper grid and roof;
Fig. 6 is a kind of hardware connection structure schematic diagram studying the experimental provision of honeybee behavioral trait under electro photoluminescence of the present invention;
Fig. 7 is slave computer software flow chart of the present invention;
Fig. 8 is upper computer software flow chart of the present invention.
Embodiment
For making to have a better understanding and awareness architectural feature of the present invention and effect of reaching, coordinating detailed description in order to preferred embodiment and accompanying drawing, being described as follows:
Study an experimental provision for honeybee behavioral trait under electro photoluminescence, described device comprises stimulation chamber, stimulates chamber to have roof and diapire.
As shown in Figure 1 and Figure 2, in stimulation chamber 1, electro photoluminescence unit, honeybee escape behavior information acquisition unit, honeybee acoustic information collecting unit is furnished with.Electro photoluminescence unit comprises the first electro photoluminescence region 11 and the second electro photoluminescence region 12; First electro photoluminescence region 11 is connected with power supply respectively with the second electro photoluminescence region 12, and the first electro photoluminescence region 11 or the break-make between the second electro photoluminescence region 12 and power supply can adopt main controller controls.Honeybee escape behavior information acquisition unit and acoustic information collecting unit are all connected with master controller.Honeybee escape behavior information acquisition unit collects honeybee C position and is transferred to master controller, and master controller determines the power supply in connection first electro photoluminescence region 11 or the second electro photoluminescence region 12 according to the honeybee position collected.Acoustic information collecting unit collects the voice signal of honeybee C and is transferred to master controller, and master controller processes voice signal.
In order to avoid electro photoluminescence unit exists blind area, the first electro photoluminescence region 11 is divided into first to power on stimulated zone 111 and first time electro photoluminescence region 112; Second electro photoluminescence region 12 is divided into second to power on stimulated zone 121 and second time electro photoluminescence region 122; First power on stimulated zone 111 and second power on stimulated zone 121 be fixed on stimulate chamber 1 roof inside; First time electro photoluminescence region 112 and second time electro photoluminescence region 122 are fixed on the inside bottom wall stimulating chamber 1.
Provided by the invention first stimulated zone 111 and second that powers on powers on stimulated zone 121 area sum for stimulate area inside chamber 1 roof; First time electro photoluminescence region 112 and second time electro photoluminescence region 122 area sum are for stimulate chamber 1 inside bottom wall area.Design like this, no matter honeybee C is stimulating which position in chamber, and no matter honeybee to creep inside roof or inside bottom wall is creeped, as long as energising, can carry out electro photoluminescence experiment, there will not be blind area to honeybee C.
As shown in Figure 3, first stimulated zone 121, the second time electro photoluminescence region 122 that power on, stimulated zone 111, first time electro photoluminescence region 112, second that power on includes positive pole a, negative pole b, and positive pole a, negative pole b are staggered formation lattice-shaped.The first positive pole a in positive pole a and first time electro photoluminescence region 112 powering on stimulated zone 111 is electrically connected; The second positive pole a in positive pole a and second time electro photoluminescence region 122 powering on stimulated zone 121 is electrically connected.
As shown in Figure 4, Figure 5, honeybee escape behavior information acquisition unit comprises multiple infrared transmitting tube 21 and the multiple infrared receiving tubes 22 corresponding with multiple infrared transmitting tube 21, and multiple infrared transmitting tube 21 is fixed on the same side in the first electro photoluminescence region 11 and the second electro photoluminescence region 12.Multiple infrared receiving tube 22 is separately fixed at the opposite side in the first electro photoluminescence region 11 and the second electro photoluminescence region 12, and multiple infrared transmitting tube 21 and multiple infrared receiving tube 22 are symmetrical arranged.The symmetric points line of multiple infrared transmitting tube 21 and multiple infrared receiving tube 22 runs through the first electro photoluminescence region 11 and the second electro photoluminescence region 12.
Honeybee acoustic information collecting unit comprises microphone 31 and filter circuit; Microphone 31 is fixed on to stimulate in chamber 1, and microphone 31 changes voice signal into the signal of telecommunication and is transferred to filter circuit, and filter circuit is by the electrical signal transfer after filtration to master controller, and analog-signal transitions is data signal by master controller.
As shown in Figure 6, master controller is connected with computer B; The honeybee positional information received and acoustic information are transferred to computer B and preserve by master controller, to show various information, and subsequent data analysis.
Stimulate the size of chamber and shape experimentally can require difference and different, the present embodiment describes in detail for the stimulation chamber being of a size of 152mm ╳ 29mm ╳ 6mm (chamber inside dimension).
As shown in Figure 1, stimulate chamber 1 to be made up of transparent organic glass, on chamber, have a diameter to be the honeybee entrance 41 of 10mm, honeybee entrance 41 is for putting into or take out tested honeybee C.Honeybee entrance 41 has lid 42 to be covered with at ordinary times, and lid 42 is made up of transparent organic glass.
Stimulate chamber stereochemical structure to split schematic diagram as shown in Figure 1 and Figure 2, because the height of health when housing depth and honeybee creep is suitable, honeybee can not fly in chamber, can only creep back and forth in cavity.Stimulate in chamber and arrange electro photoluminescence unit, honeybee escape behavior information acquisition unit and honeybee acoustic information collecting unit.
Electro photoluminescence unit is stimulate inside the roof of chamber and stimulate inside chamber bottom, and the grid that fixing copper wire is made, the diameter of copper wire is 1.0mm, and grid distance is 1.0mm, and the width of grid is equal with cuboid chamber, and grid length is that rectangular cavity is identical.Both positive and negative polarity is staggered as shown in Figure 4, and the positive pole a of grid copper wire is in centre position, and disconnection divide into left and right two parts, and left and right two parts are the first above-mentioned electro photoluminescence region 11 and the second electro photoluminescence region 12.Roof copper grid and the unique difference of diapire copper grid are: the honeybee entrance 41 having 10mm in the middle of roof.Because the width of copper cash is 1mm, gap is also 1mm, and the height of honeybee is at 8-20mm.So when the positive pole a in the first electro photoluminescence region 11 or the second electro photoluminescence region 12 and 3-24V adjustable power of direct current A positive pole are connected, the honeybee in this region, no matter be creep at roof or creep at diapire all can be subject to electro photoluminescence, the honeybee being subject to electro photoluminescence can flee from electro photoluminescence region fast, another region be not energized in final arrival chamber.The presence or absence of the electro photoluminescence in two regions, and time length main controller controls.
As shown in Fig. 2, Fig. 4, Fig. 5, honeybee escape behavior information acquisition unit is stimulate inside the long limit of chamber, 24 pairs of infrared tubes of equidistant arrangement.Wherein while be infrared transmitting tube 21, another side is corresponding is with it infrared receiving tube 22, and the axis of symmetry of 24 pairs of infrared tubes runs through the first electro photoluminescence region 11 and the second electro photoluminescence region 12, and namely the axis of symmetry is parallel to the long limit stimulating chamber 1.The model of infrared transmitting tube 21 is IR928-6C-F, and the model of infrared receiving tube 22 is PT928-6C-F.When honeybee is in cavity in motion process, pass through or rest on certain position, the health of honeybee C can block the light that corresponding position infrared transmitting tube 21 sends, the infrared light that the infrared transmitting tube 21 that in this position cavity, the infrared receiving tube 22 of side does not receive offside sends, this receiving tube output low level.When honeybee C leaves certain position, the infrared receiving tube 22 of chamber side just can receive the infrared light sent from offside infrared transmitting tube 21 herein, i.e. infrared receiving tube 22 conducting export high level.The signal that the comprehensive 24 pairs of infrared receiving tubes 22 export, just can the quantitative study honeybee at a time position of honeybee C and motor behavior.
Honeybee sound collection unit comprises miniature electret microphone 31, the Butterworth second-order filter circuit that model is 3015.When honeybee is subject to electro photoluminescence, can sound, frequency is at below 600Hz, sound is received by Electret condenser microphone 31, and voice signal dress is changed into the signal of telecommunication, the Butterworth second-order filter circuit that the signal of telecommunication after changing is 1000Hz by cut-off frequency, the clutter of more than 1000Hz is removed, the signal removing clutter, with the a/d converter of the sampling frequency of 10kHz, converts data signal to, then passes to computer B by USB interface.
Master controller is the ATxmega64A3U of Atmel company, containing abundant I/O port, first the power on positive pole a in positive pole a and first time electro photoluminescence region 112 of stimulated zone 111 and the connection of adjustable power of direct current is controlled by the same I/O port of master controller, second the power on positive pole a in stimulated zone 121 and second time electro photoluminescence region 122 and the connection of adjustable power of direct current is controlled by another I/O port of master controller, the electro photoluminescence so controlling the first electro photoluminescence region 11 and the second electro photoluminescence region 12 is with or without, and stimulation time.The I/O port of infrared receiving tube 22 output and main controller is electrically connected simultaneously.Conducting when infrared receiving tube 22 receives infrared light, exports high level.Export when infrared receiving tube 22 does not receive infrared light as low level, high level and low level are all transferred to master controller by I/O port and finally pass to computer B and carry out analyzing, show, preserve.The signal of Electret condenser microphone 31 processes after filtering and is electrically connected with the AD functional module of master controller, and analog signal is converted to data signal.With USB functional module in main controller, communicated with computer B by USB interface, receive the stimulation number of times that computer B transmits, transmit positional information and the acoustic information of honeybee simultaneously to computer B.
Software section mainly comprises the slave computer software on master controller and the upper computer software on computer B.Slave computer software flow chart as shown in Figure 7, is responsible for controlling electro photoluminescence, is gathered the positional information calculating honeybee, gather the acoustic information of honeybee, and information is passed to host computer.Upper computer software flow chart as shown in Figure 8, is responsible for the communication of master controller and computer B, stimulation number of times is passed to master controller, receives simultaneously, display, preserves and the positional information of analyzing and processing honeybee and acoustic information.
The use of device: tackle a honeybee from beehive mouth, honeybee is put into stimulation chamber, regulates dc source, is transferred to the voltage swing needing to stimulate, the number of times of input stimulus on upper computer software, clicks and start, and namely device starts electro photoluminescence experiment.Device can detect honeybee position by infrared tube, and according to the honeybee position detected, gives honeybee electro photoluminescence in honeybee region, and the electric stimulating time at every turn given is 10s.The time interval between twice electro photoluminescence is 20s, until reach the stimulation number of times of setting, completes electro photoluminescence experiment.Complete to stimulation number of times from click, the infrared tube of device is gathering the positional information of honeybee always, and in device, Electret condenser microphone 31 is gathering honeybee positional information always.And these information are transferred to computer B by USB.Experimenter can know the behavioral trait of honeybee to respective intensities electro photoluminescence by the positional information and acoustic information analyzing honeybee.
More than show and describe general principle of the present invention, principal character and advantage of the present invention.The technical staff of the industry should understand; the present invention is not restricted to the described embodiments; the just principle of the present invention described in above-described embodiment and specification; the present invention also has various changes and modifications without departing from the spirit and scope of the present invention, and these changes and improvements all fall in claimed scope of the present invention.The protection domain of application claims is defined by appending claims and equivalent thereof.
Claims (10)
1. study an experimental provision for honeybee behavioral trait under electro photoluminescence, it is characterized in that: described device comprises stimulation chamber; Electro photoluminescence unit, honeybee escape behavior information acquisition unit, honeybee acoustic information collecting unit is furnished with in described stimulation chamber; Described electro photoluminescence unit comprises the first electro photoluminescence region and the second electro photoluminescence region; Described first electro photoluminescence region is connected with power supply respectively with the second electro photoluminescence region; Described first electro photoluminescence region or the break-make between the second electro photoluminescence region and described power supply adopt main controller controls; Described honeybee escape behavior information acquisition unit is connected with described master controller; Described honeybee escape behavior information acquisition unit collects honeybee position and is transferred to master controller; Described master controller according to described in the honeybee position that collects determine the power supply in connection first electro photoluminescence region or the second electro photoluminescence region; Described acoustic information collecting unit is connected with described master controller; Described acoustic information collecting unit collects the voice signal of honeybee and is transferred to described master controller.
2. a kind of experimental provision studying honeybee behavioral trait under electro photoluminescence according to claim 1, is characterized in that: described stimulation chamber has roof and diapire.
3. a kind of experimental provision studying honeybee behavioral trait under electro photoluminescence according to claim 2, is characterized in that: described first electro photoluminescence region is divided into first to power on stimulated zone and first time electro photoluminescence region; Second electro photoluminescence region is divided into second to power on stimulated zone and second time electro photoluminescence region; Described first stimulated zone and second stimulated zone that powers on that powers on is fixed on inside the roof of described stimulation chamber; Described first time electro photoluminescence region and second time electro photoluminescence region are fixed on the inside bottom wall of described stimulation chamber.
4. a kind of experimental provision studying honeybee behavioral trait under electro photoluminescence according to claim 3, is characterized in that: described first stimulated zone and the described second stimulated zone area sum that powers on that powers on is area inside described stimulation chamber roof; Described first time electro photoluminescence region and described second time electro photoluminescence region area sum are area inside described stimulation chamber bottom.
5. a kind of experimental provision studying honeybee behavioral trait under electro photoluminescence according to claim 3, is characterized in that: described first power on stimulated zone, the second time electro photoluminescence region of stimulated zone, first time electro photoluminescence region, second that power on includes positive pole, negative pole; Described positive pole, negative pole are staggered formation lattice-shaped.
6. a kind of experimental provision studying honeybee behavioral trait under electro photoluminescence according to claim 5, is characterized in that: the described first the power on positive pole of stimulated zone and positive pole in described first time electro photoluminescence region is electrically connected; Described second the power on positive pole of stimulated zone and the positive pole in described second time electro photoluminescence region is electrically connected.
7. a kind of experimental provision studying honeybee behavioral trait under electro photoluminescence according to claim 1, is characterized in that: described honeybee escape behavior information acquisition unit comprises multiple infrared transmitting tube and the multiple infrared receiving tubes corresponding with described multiple infrared transmitting tube; Described multiple infrared transmitting tube is fixed on the same side in described first electro photoluminescence region and the second electro photoluminescence region; Described multiple infrared receiving tube is separately fixed at the opposite side in described first electro photoluminescence region and the second electro photoluminescence region, and described multiple infrared transmitting tube and described multiple infrared receiving tube are symmetrical arranged.
8. a kind of experimental provision studying honeybee behavioral trait under electro photoluminescence according to claim 7, is characterized in that: the symmetric points line of described multiple infrared transmitting tube and multiple infrared receiving tube runs through described first electro photoluminescence region and the second electro photoluminescence region.
9. a kind of experimental provision studying honeybee behavioral trait under electro photoluminescence according to claim 1, is characterized in that: described honeybee acoustic information collecting unit comprises microphone and filter circuit; Described microphone is fixed in described stimulation chamber; Described microphone changes voice signal into the signal of telecommunication and is transferred to filter circuit; Filtered electrical signal transfer is given described master controller by described filter circuit; Analog-signal transitions is data signal by described master controller.
10. a kind of experimental provision studying honeybee behavioral trait under electro photoluminescence according to claim 1, is characterized in that: described master controller is connected with computer; The honeybee positional information received and acoustic information are transferred to computer by described master controller.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110521675A (en) * | 2019-09-18 | 2019-12-03 | 清华大学 | Y type labyrinth for the experiment of honeybee praxiology research |
| CN111165433A (en) * | 2020-01-09 | 2020-05-19 | 杭州魔象智能科技有限公司 | Experimental device and experimental method for researching motion characteristics of living insect stinging needle |
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