CN105453752A - Method for promoting rapid germination of rhizoma paridis seeds - Google Patents
Method for promoting rapid germination of rhizoma paridis seeds Download PDFInfo
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- CN105453752A CN105453752A CN201510816376.4A CN201510816376A CN105453752A CN 105453752 A CN105453752 A CN 105453752A CN 201510816376 A CN201510816376 A CN 201510816376A CN 105453752 A CN105453752 A CN 105453752A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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Abstract
The invention discloses a method for promoting rapid germination of rhizoma paridis seeds. The method is implemented mainly through the following steps: firstly washing fresh rhizoma paridis seeds, breaking dormancy by high temperature and promoting seed germination by using a chemical reagent. A hydroponics nutrient solution is used to promote germination and growth of seeds in the later stage, and seed dormancy breaking and integration of water and fertilizer are combined to a system, thus providing an approach to integration of water and fertilizer of rhizoma paridis seeds. The method is easy to operate and is suitable for large-scale seedling growing. By the method, seedling emergency of rhizoma paridis seeds in the next year after harvesting can be realized and is accomplished one year earlier than natural seedling emergency, seed seedling growing efficiency is raised, and seedling growth and rhizoma paridis growth cycles are shortened. The invention provides a certain technical support for industrial development of rhizoma paridis planting industry.
Description
Technical field
The invention belongs to medicinal material planting technology field, be specifically related to a kind of method promoting Paris polyphylla seed quick-speed germination.
Background technology
Paris polyphylla is Liliales Trilliaceae (Trilliaceae) Paris (Paris) plant.The whole world has 24 kinds, and China has 19 kinds, and wherein the Paris polyphylla kind in Yunnan occupies the hat in the whole nation, has 14 kinds.Paris polyphylla is Chinese medicine important simply, there is clearing heat and detoxicating, Sweeling-eliminating medicine powder stasis of blood effect, in order to treat venomous snake bite, furuncle swelling toxin, mumps etc., traditional medicine is mainly used in various sore, snake venom, ulcer, and modern pharmacological research proves that it has the effects such as anticancer, antimicrobial, antifertility, calmness, analgesia, relieving cough and asthma, hemostasis.Paris polyphylla is the important source material that life pellet, Yunnan Baiyao, Gong Xue Ning and other preparations taken by force by Chinese patent drug simultaneously.Along with the fast development of Chinese Medicine Industry, significantly increase progressively every year the demand of Paris polyphylla, every annual requirement is at more than 4000t, and annual increasing with 20% demand.In recent years, along with the increase to Paris polyphylla demand, the stock number of wild Paris polyphylla sharply reduces, and traditional artificial propagation cost drops into larger, the factors such as Paris polyphylla production environment exists selectivity by force in addition, and growth cycle is long, and the Paris polyphylla seed dormancy phase is long cause Paris polyphylla to plant industry development facing lots of obstruction.
At present about the modes of reproduction of Paris polyphylla, be domesticly mainly seed sexual propagation and rhizome stripping and slicing is nourished and generated, its propagation method is as follows:
Seed sexual propagation: after 10 to November Yunnan Paris polyphylla seed maturity, seed removes shell after plucking, and cleans and dries steam, carry out vernalization process by one deck matrix one deck seed storage in indoor.Husky Tibetan vernalization is adopted to carry out seed treatment, then drilling or disseminate on ready-made seedbed, the wide 120cm in seedbed, high 20cm, furrow width 30 centimetres, cover humus soil and the ash of 1:1 above, thick about 1.5 centimetres of earthing, then on moisture in the soil face lid layer pine needle or broken grass, water permeable, keep moistening, in April, the 3rd receives seedling.
Band terminal bud rhizome cutting propagation: winter is after Yunnan Paris polyphylla falls seedling, get Yunnan rhizoma paris rhizome, press perpendicular to rhizome major axes orientation, long 3 ~ 4cm place cutting (remainder can be used as commodity Paris polyphylla and sells) is saved to be with terminal bud part, wound dips in ash or quicklime, press field planting seeding row spacing 15cm × 15cm subsequently to cultivate, the 2nd year spring just can emerge.
Paris polyphylla seed has the physiological property of embryo after-ripening, needs under field conditions (factors) could sprout through the time in two winters, and emergence rate only has about 10%, and seedling-growing time is long, and emergence rate is low; Some Domestic patent of invention or method part solve the problem of Yunnan Paris polyphylla seed germination difficulty, but there are the following problems: seed advanced decay is serious, germination rate is low, operation is too complicated or require too highly in actual production, to be difficult to scale to implement; Secondly rhizome segment plantation, a large amount of production raw material can be taken, cause cultivation to plant the aggravation of contradictions with pharmaceutical raw material, the rhizome in addition after segment, due to land for growing field crops moisture and difficult control of temperature, easy infection is gone rotten, and germplasm materials percent of loss is up to more than 50%, and reproduction rate is low, emerge irregular, inconvenience management, planting cost is also high, is unfavorable for the spread that Paris polyphylla is planted.
There are long kind medicine and medication tradition in Wenshan Prefecture, and unique national culture is that solid foundation has been established in the development of nationality medicine, and being one of area of Yunnan Province's Paris polyphylla plantation, is the important source material base of development " cloud medicine " industry.Solving the task of top priority of Paris polyphylla plantation industry development obstacle is exactly to solve the obstacle on Paris polyphylla seedling growing process.For this reason, we are badly in need of to provide a kind of new approaches to the quick cultivating seedling growing process of Paris polyphylla.
Summary of the invention
In order to solve the above-mentioned technical problem existed in prior art, the object of the invention is to provide a kind of method promoting Paris polyphylla seed quick-speed germination, and it has, and growing-seedling period is short, germination rate is high, cost is low, and shoot survival percent is high, easily with features such as large-scale productions.
The present invention realizes especially by following technical scheme:
Promote a method for Paris polyphylla seed quick-speed germination, comprise the following steps:
1) select to gather then Yunnan Paris polyphylla seed, remove by damage by disease and insect and immature seed, remove arillus, clean;
2) clean seed is placed in 38 ~ 42 DEG C of water-baths to soak, soak time 2 ~ 10h;
3) the Paris polyphylla seed interval, Yunnan after soaking is laid in uniformly is covered with 2 ~ 3 centimetres of husky receptacle, seed covers the river sand of 3 ~ 5 centimetres, keep moistening;
4) spray concentration is 2.78 × 10
-4~ 5.58 × 10
-4the 6-Furfurylaminopurine of mol/L and 0.99 × 10
-2~ 4.9 × 10
-2the potassium nitrate solution of mol/L, 17 ~ 24 DEG C of placements;
5), after 5 ~ 15 days, arrange according to the husky one deck seed of one deck in cultivation circulating frame, keep soil humidity 30%-40%, and spray Gram nutrient solution suddenly;
6) keep moistening, the river sand 0.5 centimetre of variable color in surface bleaches and just starts to drench nutrient solution, within 20 ~ 60 days, sprouts, within 30 ~ 90 days, grows radicle.
Further, step (1) is specially the ripe Yunnan Paris polyphylla red-coloured seeds of gathering, and puts shady and cool place's after-ripening 10 ~ 20 days, then puts into water and wash arillus by rubbing with the hands, and impurity is removed in rinsing, takes out the clean seed of sinking.
Further, step (2) is specially and clean seed is placed in water-bath, and temperature is set to 38-42 DEG C, tepidarium 6 hours, makes water logging not have all seeds.
Further, the density that in step (3), Yunnan Paris polyphylla seed tiles is for being 342/dm
2, the humidity of sand remains on 40% ~ 50%.
Further, after step (5) is specially 5 ~ 15 days, then start to spread seed with the sand cultivating circulating frame and to spread in bottom 2 ~ 3 centimetres, paving one deck kind subcovering one deck is husky, sand was advisable to cover seed, and seed is no more than five layers, finally adds a cover the sand of 3 ~ 5 centimetres, keep soil humidity at 30%-40%, spray Gram nutrient solution suddenly according to the moistening degree of river sand every 5-10 days.
Preferably, a kind of method promoting Paris polyphylla seed quick-speed germination, comprises the following steps:
1) select the ripe Yunnan Paris polyphylla red-coloured seeds of gathering then, remove by damage by disease and insect and immature seed, put shady and cool place's after-ripening 10 ~ 20 days, then put into water and wash arillus by rubbing with the hands, impurity is removed in rinsing, takes out the clean seed of sinking;
2) clean seed is placed in 38 ~ 42 DEG C of water-baths to soak, makes water logging not have all seeds, warm water invades bubble 6h;
3) be laid in uniformly at the Paris polyphylla seed interval, Yunnan after soaking and be covered with 2 ~ 3 centimetres of husky receptacle, seed density is 342/dm
2, seed covers the river sand of 3 ~ 5 centimetres, the husky humidity of river sand remains on 40% ~ 50%;
4) spray concentration is 2.78 × 10
-4~ 5.58 × 10
-4the 6-Furfurylaminopurine of mol/L and 0.99 × 10
-2~ 4.9 × 10
-2the potassium nitrate solution of mol/L, 18 ~ 24 DEG C of placements;
5) after 5 ~ 15 days, then start to spread seed with the sand cultivating circulating frame and to spread in bottom 2 ~ 3 centimetres, paving one deck kind subcovering one deck is husky, sand was advisable to cover seed, seed is no more than five layers, finally add a cover the sand of 3 ~ 5 centimetres, keep soil humidity at 30%-40%, sprayed Gram nutrient solution suddenly every 5-10 days;
6) keep moistening, the river sand 0.5 centimetre of variable color in surface bleaches and just starts to drench Gram nutrient solution suddenly, within 20 ~ 60 days, sprouts, within 30 ~ 90 days, grows radicle.
Beneficial effect of the present invention is: the present invention is simple and easy to do, with low cost, and the budding period of Yunnan Paris polyphylla was shortened to 4 ~ 8 months from 2 years, and germination rate brings up to 70 ~ 90% from 30 ~ 50%.The breeding that the present invention is Yunnan Paris polyphylla and large-scale planting provide technical guarantee.
Embodiment
Below in conjunction with embodiment, the present invention is described further, the following stated, only to preferred embodiment of the present invention, not do other forms of restriction to the present invention, any those skilled in the art may utilize the technology contents of above-mentioned announcement to be changed to the Equivalent embodiments of equal change.Everyly do not depart from the present invention program's content, any simple modification done following examples according to technical spirit of the present invention or equivalent variations, all drop in protection scope of the present invention.
Embodiment 1
Paris polyphylla fresh seed in Yunnan is bought in Maguan County Xu Laobanchu on September 20th, 2014, through academy of agricultural sciences of Wenshan Prefecture, biological Paris polyphylla seminar of institute and Chinese Academy of Sciences Kunming Institute of Zoology teacher Ji Yunheng are accredited as the fresh seed of maturation of Trilliaceae plant Yunnan Paris polyphylla, test and carry out in biotechnology research institute of Wenshan Prefecture of Yunnan Province laboratory year December in October, 2014 to 2014.
Promote a method for Paris polyphylla seed quick-speed germination, specifically comprise following steps:
1) adopt the ripe fresh seeds 500g of Yunnan Paris polyphylla, shell, cleaning seed removes seed coat.
2) number gets clean fresh seeds 900 three parts respectively, is labeled as A1 respectively, A2, A3, installs respectively with mesh bag.
3) seed cleaned is placed in water-bath, temperature is set to 38-42 DEG C, tepidarium 6 hours, makes water logging not have all seeds.
3) water-bath is complete, seed is placed in the basin having spread 5 centimetres of river sands, and every part of seed puts into a basin respectively, sprays reagent A (3.25 × 10
-4mol/L6-chaff aminopurine, 2.18 × 10
-2the potassium nitrate mixed solution of mol/L), make seed fully moistening.
4) cover the river sand of 3 centimetres, seal pelvic surface of sacrum with preservative film, cultivate in the incubator putting into temperature 18-24 DEG C, within second day, take off film, the river sand on surface is removed, and spray reagent A, makes seed fully moistening, then cover river sand and preservative film, later every day repeats, until take off film after 7-10.
5) be seeded in after taking off film in Turnround basket, pad last layer mesh bag, spread 5 centimetres of river sands, then tile seed, covers the river sand of 3 centimetres, be placed on the seedling rearing room of temperature 20-25 DEG C, keep river sand humidity 30%-40%, until Seed sprouting, namely seed one end starts there is projection, every 7-10 days drenches Gram nutrient solution suddenly and once, keeps river sand moisturizing.
6) within after taking off film 30 days, rate (referring to that seed one end starts to occur that haloing is sprouted to there being protrusion all to belong to) of sprouting is added up, within 60 days, 90 days, add up germination rate (referring to that root breakthrough protrusion has just started to see that the tip of a root stretches out to radicle plumular axis and all belonged to germination) respectively, shown in result table 1.
Table 1 Seed sprouting rate, germination rate are added up
| 30d is sprouted rate (%) | 60d germination rate (%) | 90d germination rate (%) | |
| A1 | 89.01 | 65.00 | 85.10 |
| A2 | 85.55 | 62.25 | 89.80 |
| A3 | 82.50 | 60.89 | 82.24 |
Embodiment 2
Tall building, Yunnan ten thousand comprehensive agricultural development Co., Ltd is adopted to provide seed, band 200 jin, shell.
1) select Yunnan Paris polyphylla fresh seeds, shell, cleaning seed removes seed coat, removes by damage by disease and insect and immature seed, removes arillus, clean, and washing seed to the greatest extent has 67 jin.
2) be placed in 38 ~ 42 DEG C of warm water soak washing most seed, soak time 6 hours, makes water logging not have all seeds.
3) river sand of layer overlay 2 cm thick in a flat container, river sand is put one deck filter paper, then after water-bath Paris polyphylla seed be sprinkling upon uniformly on filter paper, seed tiling can not be in heaps.
4) 2.78 × 10 are sprayed to seed
-4mol/L6-chaff aminopurine and 3.8 × 10
-2the potassium nitrate mixed solution of mol/L, then mixes thoroughly with river sand, and uniform spreading, in container, at the thin sand of upper cover last layer, sprays same solution every day once to keep moistening, keeps temperature 18-23 DEG C, places 8 days.
5) after 8 days, seed broadcasting in the screen frame of river sand spreading 5 cm thicks, topmost one deck covers the river sand of 4cm left and right thickness, keeps soil humidity 30% ~ 40%, be placed on indoor, drench Hoagland ' s nutrient solution according to the moistening degree of river sand every 5 ~ 10 days.
Present case due to grain weight large, take the mode of sample investigation, method is as follows: therefrom optional 3 jin of seeds are sowed in three frames respectively, and be labeled as respectively before B1, B2, B3 sow and count respectively, add up germination rate after 100 days, result is as table 2.
Table 2 percentage of seedgermination is added up
| Repeat | B1 | B2 | B3 |
| The germination rate (%) of after planting 100 days | 73.92 | 85.12 | 80.00 |
Claims (6)
1. promote a method for Paris polyphylla seed quick-speed germination, it is characterized in that, comprise the following steps:
1) select to gather then Yunnan Paris polyphylla seed, remove by damage by disease and insect and immature seed, remove arillus, clean;
2) clean seed is placed in 38 ~ 42 DEG C of water-baths to soak, soak time 2 ~ 10h;
3) the Paris polyphylla seed interval, Yunnan after soaking is laid in uniformly is covered with 2 ~ 3 centimetres of husky receptacle, seed covers the river sand of 3 ~ 5 centimetres, keep moistening;
4) spray concentration is 2.78 × 10
-4~ 5.58 × 10
-4the 6-Furfurylaminopurine of mol/L and 0.99 × 10
-2~ 4.9 × 10
-2the potassium nitrate solution of mol/L, 18 ~ 24 DEG C of placements;
5), after 5 ~ 15 days, arrange according to the husky one deck seed of one deck in cultivation circulating frame, keep soil humidity 30%-40%, and spray Gram nutrient solution suddenly;
6) keep moistening, the river sand 0.5 centimetre of variable color in surface bleaches and just starts to drench nutrient solution, within 20 ~ 60 days, sprouts, within 30 ~ 150 days, grows radicle.
2. a kind of method promoting Paris polyphylla seed quick-speed germination according to claim 1, it is characterized in that: step (1) is specially the ripe Yunnan Paris polyphylla red-coloured seeds of gathering, put shady and cool place's after-ripening 10 ~ 20 days, put into water again and wash arillus by rubbing with the hands, impurity is removed in rinsing, takes out the clean seed of sinking.
3. a kind of method promoting Paris polyphylla seed quick-speed germination according to claim 1, it is characterized in that: step (2) is specially and clean seed is placed in water-bath, temperature is set to 38-42 DEG C, tepidarium 6 hours, makes water logging not have all seeds.
4. a kind of method promoting Paris polyphylla seed quick-speed germination according to claim 1, is characterized in that: in step (3), the density of Yunnan Paris polyphylla seed tiling is for being 342/dm
2, the humidity of sand remains on 40%-50%.
5. a kind of method promoting Paris polyphylla seed quick-speed germination according to claim 1, it is characterized in that: after step (5) is specially 5 ~ 15 days, then start to spread seed with the sand cultivating circulating frame and to spread in bottom 2 ~ 3 centimetres, paving one deck kind subcovering one deck is husky, sand was advisable to cover seed, and seed is no more than five layers, finally adds a cover the sand of 3 ~ 5 centimetres, keep soil humidity at 30%-40%, spray Gram nutrient solution suddenly according to the moistening degree of river sand every 5-10 days.
6. a kind of method promoting Paris polyphylla seed quick-speed germination according to any one of Claims 1 to 5, is characterized in that:
1) select the ripe Yunnan Paris polyphylla red-coloured seeds of gathering then, remove by damage by disease and insect and immature seed, put shady and cool place's after-ripening 10 ~ 20 days, then put into water and wash arillus by rubbing with the hands, impurity is removed in rinsing, takes out the clean seed of sinking;
2) clean seed is placed in 38 ~ 42 DEG C of water-baths to soak, makes water logging not have all seeds, warm water invades bubble 6h;
3) be laid in uniformly at the Paris polyphylla seed interval, Yunnan after soaking and be covered with 2 ~ 3 centimetres of husky receptacle, seed density is 342/dm
2, seed covers the river sand of 3 ~ 5 centimetres, the husky humidity of river sand remains on 40% ~ 50%;
4) spray concentration is 2.78 × 10
-4~ 5.58 × 10
-4the 6-Furfurylaminopurine of mol/L and 0.99 × 10
-2~ 4.9 × 10
-2the potassium nitrate solution of mol/L, 18 ~ 24 DEG C of placements;
5) after 5 ~ 15 days, then start to spread seed with the sand cultivating circulating frame and to spread in bottom 2 ~ 3 centimetres, paving one deck kind subcovering one deck is husky, sand was advisable to cover seed, seed is no more than five layers, finally add a cover the sand of 3 ~ 5 centimetres, keep soil humidity at 30%-40%, sprayed Gram nutrient solution suddenly every 5-10 days;
6) keep moistening, the river sand 0.5 centimetre of variable color in surface bleaches and just starts to drench Gram nutrient solution suddenly, within 20 ~ 60 days, sprouts, within 30 ~ 150 days, grows radicle.
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| CN201510816376.4A CN105453752A (en) | 2015-11-23 | 2015-11-23 | Method for promoting rapid germination of rhizoma paridis seeds |
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|---|---|---|---|
| CN201510816376.4A CN105453752A (en) | 2015-11-23 | 2015-11-23 | Method for promoting rapid germination of rhizoma paridis seeds |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106171409A (en) * | 2016-07-06 | 2016-12-07 | 李贵东 | A kind of Rhizoma Paridis virus-free fast-growing method for culturing seedlings |
| CN107079692A (en) * | 2017-04-27 | 2017-08-22 | 安徽省霍山县农伯乐开发有限公司 | A kind of Paris polyphylla seed seedling-raising method |
| CN109496492A (en) * | 2018-12-30 | 2019-03-22 | 云南明远中药材种植有限公司 | A method of promote paris polyphylla seed to sprout |
| CN112194524A (en) * | 2020-09-30 | 2021-01-08 | 云南华隆重楼种植科技有限公司 | Preparation and use methods of nutrient solution for paris polyphylla |
| CN113080001A (en) * | 2021-04-19 | 2021-07-09 | 福建坤加建设有限公司 | Garden orchid seedling growing method |
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| CN102487627A (en) * | 2011-12-09 | 2012-06-13 | 四川农业大学 | Method for effectively breaking dormancy of Paris polyphylla seeds |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106171409A (en) * | 2016-07-06 | 2016-12-07 | 李贵东 | A kind of Rhizoma Paridis virus-free fast-growing method for culturing seedlings |
| CN107079692A (en) * | 2017-04-27 | 2017-08-22 | 安徽省霍山县农伯乐开发有限公司 | A kind of Paris polyphylla seed seedling-raising method |
| CN109496492A (en) * | 2018-12-30 | 2019-03-22 | 云南明远中药材种植有限公司 | A method of promote paris polyphylla seed to sprout |
| CN112194524A (en) * | 2020-09-30 | 2021-01-08 | 云南华隆重楼种植科技有限公司 | Preparation and use methods of nutrient solution for paris polyphylla |
| CN113080001A (en) * | 2021-04-19 | 2021-07-09 | 福建坤加建设有限公司 | Garden orchid seedling growing method |
| CN113080001B (en) * | 2021-04-19 | 2023-04-07 | 福建坤加建设有限公司 | Garden orchid seedling growing method |
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Application publication date: 20160406 |