CN105434323A - Yeast fermentation compound composition and application thereof in whitening and moisturizing skincare products - Google Patents
Yeast fermentation compound composition and application thereof in whitening and moisturizing skincare products Download PDFInfo
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- CN105434323A CN105434323A CN201510980162.0A CN201510980162A CN105434323A CN 105434323 A CN105434323 A CN 105434323A CN 201510980162 A CN201510980162 A CN 201510980162A CN 105434323 A CN105434323 A CN 105434323A
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- yeast
- fermentation
- extract
- rhizoma polygonati
- polygonati odorati
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- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 238000010791 quenching Methods 0.000 description 1
- 229960001285 quercetin Drugs 0.000 description 1
- 235000005875 quercetin Nutrition 0.000 description 1
- 235000019172 retinyl palmitate Nutrition 0.000 description 1
- 229940108325 retinyl palmitate Drugs 0.000 description 1
- 239000011769 retinyl palmitate Substances 0.000 description 1
- NWMIYTWHUDFRPL-UHFFFAOYSA-N sapogenin Natural products COC(=O)C1(CO)C(O)CCC2(C)C1CCC3(C)C2CC=C4C5C(C)(O)C(C)CCC5(CCC34C)C(=O)O NWMIYTWHUDFRPL-UHFFFAOYSA-N 0.000 description 1
- 229930182490 saponin Natural products 0.000 description 1
- 150000007949 saponins Chemical class 0.000 description 1
- 235000017709 saponins Nutrition 0.000 description 1
- 238000010181 skin prick test Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 235000019333 sodium laurylsulphate Nutrition 0.000 description 1
- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
- 235000011152 sodium sulphate Nutrition 0.000 description 1
- YWIVKILSMZOHHF-QJZPQSOGSA-N sodium;(2s,3s,4s,5r,6r)-6-[(2s,3r,4r,5s,6r)-3-acetamido-2-[(2s,3s,4r,5r,6r)-6-[(2r,3r,4r,5s,6r)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2- Chemical compound [Na+].CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 YWIVKILSMZOHHF-QJZPQSOGSA-N 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 239000008117 stearic acid Substances 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 210000000434 stratum corneum Anatomy 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 230000035922 thirst Effects 0.000 description 1
- 229940042585 tocopherol acetate Drugs 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- XMLSXPIVAXONDL-UHFFFAOYSA-N trans-jasmone Natural products CCC=CCC1=C(C)CCC1=O XMLSXPIVAXONDL-UHFFFAOYSA-N 0.000 description 1
- LADGBHLMCUINGV-UHFFFAOYSA-N tricaprin Chemical compound CCCCCCCCCC(=O)OCC(OC(=O)CCCCCCCCC)COC(=O)CCCCCCCCC LADGBHLMCUINGV-UHFFFAOYSA-N 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000019155 vitamin A Nutrition 0.000 description 1
- 239000011719 vitamin A Substances 0.000 description 1
- 229940045997 vitamin a Drugs 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 239000000080 wetting agent Substances 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Biotechnology (AREA)
- Engineering & Computer Science (AREA)
- Dermatology (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses a yeast fermentation compound composition and an application thereof in whitening and moisturizing skincare products. The yeast fermentation compound composition is fermentation liquor obtained by adding a fragrant solomonseal rhizome extract and a jasmine flower extract into a yeast culture medium and performing fermentation by using yeasts. The appearance of the composition is clear and slight yellow, is unique in smell and has typical rich and pleasant fragrances of jasmine and fragrant solomonseal rhizome. The yeast fermentation compound composition is discovered to have a significant inhibitory action on tyrosinase activity and a good moisturizing effect. The fermentation compound composition can be used as an additive component, and is added into cosmetics to prepare external preparations according to a known method in the cosmetic industry, such as cosmetic dosage forms of solution, suspension, creams, emulsions, gels, powder, sprays and the like, thereby achieving effects of whitening and moisturizing.
Description
Technical field
The invention belongs to microbial fermentation technology and cosmetic technical field, particularly a kind of yeast fermentation compound and the application in whitening skin and preserving moisture skin care item thereof.
Background technology
The yeast extract produced after culture propagation contains abundant aminoacid, polypeptide, various vitamin and mineral, and these materials play a part indispensable in the treatment of skin, can be played the effects such as good whitening, moisturizing by different approaches.But yeast fermentation broth abnormal smells from the patient generally has distiller grains taste or special fermentation taste, and part population does not accept this yeast water taste, perfumer can add compound essence according to cosmetic product feature or location and carries out fragrance adjustment or cover.But often the later stage add this compound essence after abnormal smells from the patient pure and mild not or have increase zest may, cause product effect undesirable.
Current cosmetics add the production mainly synthetic and extracting from natural animal-plant of essence, spice.Because people often worry the essence of synthetic, the safety of spice, so be more prone to the essence, the perfume base that accept to obtain from natural animal-plant kind.Flos Jasmini Sambac is many at present to be extracted or organic solvent extraction jasmin essence by supercritical process, although high-efficient simple, often residual as organic solvents such as ethanol, easily brings zest to tender and lovely skin.
Summary of the invention
Primary and foremost purpose of the present invention is that the shortcoming overcoming prior art is with not enough, provides a kind of yeast fermentation compound.
Another object of the present invention is to provide the application of described yeast fermentation compound in whitening skin and preserving moisture skin care item.
Object of the present invention is achieved through the following technical solutions: a kind of yeast fermentation compound, is add in yeast culture medium by Rhizoma Polygonati Odorati extract and Flos Jasmini Sambac extract, the fermentation liquid obtained after fermenting with yeast.
Described yeast for youth must one in candida mycoderma (Candidalambica) CGMCC2.1763 and saccharomyces cerevisiae (Saccharomycescerevisiae) CGMCC2.1 or two kinds.
Described Rhizoma Polygonati Odorati extract is water extract.
Described Flos Jasmini Sambac extract is water extract.
Described Rhizoma Polygonati Odorati extract and described Flos Jasmini Sambac extract 0.01:1 ~ 100:1 proportioning in mass ratio; Preferably press 1:1 ~ 20:1 proportioning.
Described yeast culture medium is the culture medium of applicable Yeast Growth breeding.
The condition of described fermentation is the condition of applicable Yeast Growth breeding; Be preferably as follows: pH value is 4.0 ~ 7.0, mixing speed is 60 ~ 250rpm, and ventilation is 1 ~ 4VVM, and fermentation temperature is 25 DEG C ~ 30 DEG C, and fermentation time is 2 days ~ 4 days.
Described Rhizoma Polygonati Odorati extract prepares preferably by following steps: by Fragrant Solomonseal Rhizome and water mixing, and supersound extraction, then heated and boiled is extracted, concentrated, obtains Rhizoma Polygonati Odorati extract.
The condition of described supersound extraction is preferably to be extracted 30 ~ 60 minutes in 50 ~ 60 DEG C, and ultrasonic power is preferably 100 ~ 500W, is more preferably 100 ~ 300W.
Described Flos Jasmini Sambac extract prepares preferably by following steps: by dried jasmine flower and water mixing, heating infiltrates, and filters, obtains Flos Jasmini Sambac extract.
The condition that described heating infiltrates is preferably 70 ~ 90 DEG C and infiltrates 2 ~ 4h.
The preparation method of described yeast fermentation compound, comprises following steps:
(1) by Fragrant Solomonseal Rhizome and water mixing, supersound extraction, then heated and boiled is extracted, concentrated, obtains Rhizoma Polygonati Odorati extract;
(2) by dried jasmine flower and water mixing, heating infiltrates, and filters, obtains Flos Jasmini Sambac extract;
(3) above-mentioned Rhizoma Polygonati Odorati extract is mixed with Flos Jasmini Sambac extract, stir, filter, degerming, obtain mixture; Mixture is mixed with the yeast culture medium through bacteria removing, obtains fermentation medium;
(4) yeast is inoculated in fermentation medium ferments;
(5) the Preliminary fermentation liquid obtained after fermentation is carried out solid-liquid separation, get liquid, obtain yeast fermentation compound.
Fragrant Solomonseal Rhizome described in step (1) pulverizes and sieves in advance and obtains after cleaning.
Step (1) and the water described in step (3) are preferably deionized water.
The consumption of the water described in step (1) is preferably equivalent to Rhizoma Polygonati Odorati quality 5 ~ 8 times.
The condition of the supersound extraction described in step (1) is preferably in 50 ~ 60 DEG C of supersound extraction 30 ~ 60 minutes, and ultrasonic power is preferably 100 ~ 500W, is more preferably 100 ~ 300W.
The time that heated and boiled described in step (1) is extracted is preferably 30 ~ 90 minutes.
Step (1) and the filtration described in step (2) are preferably by 8 layers of filtered through gauze.
Concentrated degree described in step (1) is preferably concentrated to density 1.01 ~ 1.20; Be more preferably 1.05 ~ 1.15.
The consumption of the water described in step (2) is preferably equivalent to dried jasmine flower quality 5 ~ 10 times; Be more preferably 8 ~ 10 times.
The condition that heating described in step (2) infiltrates is preferably and infiltrates 0.5 ~ 4h in 70 DEG C ~ 100 DEG C, is more preferably and infiltrates 2 ~ 4h in 70 DEG C ~ 90 DEG C.
Rhizoma Polygonati Odorati extract described in step (3) and described Flos Jasmini Sambac extract 0.01:1 ~ 100:1 proportioning in mass ratio; Preferably press 1:1 ~ 20:1 proportioning.
Filtration described in step (3) is preferably by 0.45 μm of filtering with microporous membrane.
Add that volume is preferably described yeast culture medium volume 10 ~ 20% of mixture described in step (3).
Degerming described in step (3) is degerming or by 110 ~ 121 DEG C of process sterilizing in 15 ~ 30 minutes by 0.2 ~ 0.22 μm of filtering with microporous membrane.
Yeast culture medium described in step (3) is the culture medium of applicable yeast growth breeding, is preferably YPDA culture medium.
Yeast described in step (4) for youth must one in candida mycoderma (Candidalambica) CGMCC2.1763 and saccharomyces cerevisiae (Saccharomycescerevisiae) CGMCC2.1 or two kinds.
Yeast described in step (4) is preferably in the yeast of exponential phase.
The inoculum concentration of the yeast described in step (4) is 5 ~ 8 × 10 by the concentration of yeast in described fermentation medium
6cell/ml meter, is preferably 6 ~ 8 × 10
6cell/ml.
The condition of the fermentation described in step (4) is preferably as follows: pH value is 4.0 ~ 7.0, and mixing speed is 60 ~ 250rpm, and ventilation is 1 ~ 4VVM, and fermentation temperature is 25 DEG C ~ 30 DEG C, and fermentation time is 2 days ~ 4 days.
Solid-liquid separation described in step (5) is carried out preferably by following steps: obtain supernatant by seperator, and supernatant is again by 0.2 ~ 0.22 μm of filtering with microporous membrane removing yeast.
The yeast fermentation Chinese medicine composition containing skin-care effect Chinese medicine ingredients obtained as stated above as adding ingredient, can be applied to the production of cosmetics.According to method known in cosmetics industry yeast fermentation Chinese medicine composition of the present invention can be added in cosmetics and make external preparation, as State of cosmetics such as solution, suspension, cream kind, emulsion class, gel-like, powder, sprays.
Described yeast fermentation compound is preparing the application in whitening skin and preserving moisture skin care item.
Described yeast fermentation compound is preparing the application in whitening skin and preserving moisture skin care item, and wherein, the concentration of described yeast fermentation compound in described whitening skin and preserving moisture skin care item is mass percent 0 ~ 100%, containing endpoint value 0 and 100%; Be preferably mass percent 1 ~ 30%.
Described whitening skin and preserving moisture skin care item can also contain one in whitening agent, wetting agent, antioxidant, surfactant, alcohol compound and water or at least two kinds.
A kind of whitening skin and preserving moisture skin care item, containing above-mentioned yeast fermentation compound.
The present invention has following advantage and effect relative to prior art:
(1) Rhizoma Polygonati Odorati, there is yin nourishing, moisturize, relieving restlessness, the effect such as to quench the thirst.Rhizoma Polygonati Odorati is called as top grade in Compendium of Material Medica, is the demulcen of YIN nourishing panacea, medicine-food two-purpose.Modern medicine study also shows, Rhizoma Polygonati Odorati rich in proteins, crude fibre, nicotinic acid, convallarin, convallamarin, kaempferol, Quercetin, phlegmatic temperament, carbohydrate, vitamin A, zinc, manganese etc.Obvious to skin nourishment, whitening function.Flos Jasmini Sambac, pungent, sweet, cool, there is heat-clearing and toxic substances removing, dampness removing effect.Main containing benzyl alcohol or its lipid, jasmone, linalool, benzoic acid Lignum cinnamomi camphorae alcohol ester, jasmine lactone etc. in Flos Jasmini Sambac volatile material.Have and improve skin, moisten the effect of moisturizing skin, pleasant aroma, is easy to allow people accept simultaneously.The present invention is directly with the effective ingredient that water leaches from Flos Jasmini Sambac, Rhizoma Polygonati Odorati plant cell, biofermentation technique life is utilized to allow it coexist with yeast further, find that it does not have inhibition to yeast, yeast cometabolism biosynthesis pathway may be stimulated on the contrary to change, thus change fermented liquid abnormal smells from the patient, greatly reduce after fermentation that yeast fermentation broth is original distiller grains taste or special fermentation taste, overcome current yeast fermentation broth abnormal smells from the patient bad, or the defect easily causing zest to increase after adding jasmin essence.Culture propagation compound clear appearance provided by the invention, color are colourless or slightly micro-Huang, and abnormal smells from the patient uniqueness has the typical fragrance of jasmine and Rhizoma Polygonati Odorati concurrently, and fragrance enriches pleasant.The active substance contained by Rhizoma Polygonati Odorati can be made to transform further under fermentative microorganism effect simultaneously, as saponins macromolecular substances is converted into less sapogenin molecule etc., make nourishing yin effect stronger, more outstanding to skin effect.Result of the present invention also finds in surprise, the fermentation composition that Flos Jasmini Sambac extracting solution, Rhizoma Polygonati Odorati extracting solution are prepared through the process of yeast co-fermentation, add in cosmetics, cosmetics whitening skin and preserving moisture effect can be significantly improved, there is the effect of more detailed nourishing, skin whitening.
(2) the present invention provides a kind of new developing direction for Cosmetic Market.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited thereto.
Embodiment 1
(1) Rhizoma Polygonati Odorati is cleaned, pulverized 60 mesh sieves, add the deionized water being equivalent to Rhizoma Polygonati Odorati 5 times of quality, 50 DEG C ultrasonic (120W) extract 30 minutes, then heated and boiled extracts 60 minutes, 8 layers of filtered through gauze, are concentrated to density 1.08 (measure when temperature 60 C and obtain), obtain Rhizoma Polygonati Odorati extract.
(2) by the ratio mixing of dried jasmine flower and deionized water 1:8 in mass ratio, infiltrate in 80 DEG C and extract 2h, 8 layers of filtered through gauze, obtain filtrate.
(3) by above-mentioned Rhizoma Polygonati Odorati extract and Flos Jasmini Sambac extract in mass ratio 1:1 mix, stir, through 0.45 μm of filtering with microporous membrane, through 121 DEG C of sterilizings 20 minutes, naturally cool to room temperature.
(4) will be inoculated in yeast complete medium (YPDA culture medium) by the cultured saccharomyces cerevisiae of shaking table (Saccharomycescerevisiae) CGMCC2.1 in advance, add the mixture that step (3) obtains again, add that volume is equivalent to yeast complete medium volume 1/10 of mixture, makes yeast concentration be 8 × 10
6cell/ml, ferments, and fermentation parameter is pH value is 4.0 ~ 7.0, and mixing speed is 60 ~ 250rpm, and ventilation is 1 ~ 4VVM, and fermentation temperature is 25 DEG C ~ 30 DEG C, and fermentation time is 3 days.
(5) be separated by the Preliminary fermentation liquid seperator obtained after fermentation (butterfly seperator, 6500 revs/min at full speed), collecting separation of supernatant, through 0.2 μm of filtering with microporous membrane, is yeast fermentation compound.Said composition clear appearance color is colourless, and abnormal smells from the patient uniqueness has the typical fragrance of jasmine and Rhizoma Polygonati Odorati concurrently, and fragrance enriches pleasant.
Embodiment 2
(1) Rhizoma Polygonati Odorati is cleaned, pulverized 60 mesh sieves, add the deionized water being equivalent to Rhizoma Polygonati Odorati 5 times of quality, 60 DEG C ultrasonic (120W) extract 60 minutes, then heated and boiled extracts 60 minutes, 8 layers of filtered through gauze, are concentrated to density 1.11 (measure when temperature 60 C and obtain), obtain Rhizoma Polygonati Odorati extract.
(2) by the ratio mixing of dried jasmine flower and deionized water 1:10 in mass ratio, infiltrate 4h in 70 DEG C, 8 layers of filtered through gauze, obtain filtrate.
(3) by above-mentioned Rhizoma Polygonati Odorati extract and Flos Jasmini Sambac extract in mass ratio 5:1 mix, stir, through 0.45 μm of filtering with microporous membrane, through 121 DEG C of sterilizings 30 minutes, naturally cool to room temperature.
(4) will be inoculated in yeast complete medium (YPDA culture medium) by the cultured saccharomyces cerevisiae of shaking table (Saccharomycescerevisiae) CGMCC2.1 in advance, add the mixture that step (3) obtains again, add that volume is equivalent to yeast complete medium volume 1/5 of mixture, makes yeast concentration be 7 × 10
6cell/ml, ferments, and fermentation parameter is pH value is 4.0 ~ 7.0, and mixing speed is 60 ~ 250rpm, and ventilation is 1 ~ 4VVM, and fermentation temperature is 25 DEG C ~ 30 DEG C, and fermentation time is 3 days.
(5) be separated by the Preliminary fermentation liquid butterfly seperator obtained after fermentation, collecting separation of supernatant, through 0.2 μm of filtering with microporous membrane, is yeast fermentation compound.
Embodiment 3
(1) Rhizoma Polygonati Odorati is cleaned, pulverized 60 mesh sieves, add the deionized water being equivalent to Rhizoma Polygonati Odorati 8 times of quality, 50 DEG C ultrasonic (200W) extract 30 minutes, then heated and boiled extracts 90 minutes, 8 layers of filtered through gauze, are concentrated to density 1.15 (measure when temperature 60 C and obtain), obtain Rhizoma Polygonati Odorati extract.
(2) by the ratio mixing of dried jasmine flower and deionized water 1:8 in mass ratio, infiltrate 3h in 90 DEG C, 8 layers of filtered through gauze, obtain filtrate.
(3) by above-mentioned Rhizoma Polygonati Odorati extract and Flos Jasmini Sambac extract in mass ratio 10:1 mix, stir, through 0.45 μm of filtering with microporous membrane, through 115 DEG C of sterilizings 15 minutes, naturally cool to room temperature.
(4) will the cultured youth of shaking table must be inoculated in yeast complete medium (YPDA culture medium) by candida mycoderma (Candidalambica) CGMCC2.1763 in advance, add the mixture that step (3) obtains again, add that volume is equivalent to yeast complete medium volume 15% of mixture, makes yeast concentration be 8 × 10
6cell/ml, ferments, and fermentation parameter is pH value is 4.0 ~ 7.0, and mixing speed is 60 ~ 250rpm, and ventilation is 1 ~ 4VVM, and fermentation temperature is 25 DEG C ~ 30 DEG C, and fermentation time is 2 days.
(5) the Preliminary fermentation liquid butterfly seperator obtained after fermentation is separated, collects separation of supernatant, through 0.2 μm of filtering with microporous membrane, obtain yeast fermentation compound.
Embodiment 4
(1) Rhizoma Polygonati Odorati is cleaned, pulverized 60 mesh sieves, add the deionized water being equivalent to Rhizoma Polygonati Odorati 6 times of quality, 60 DEG C ultrasonic (200W) extract 60 minutes, then heated and boiled extracts 90 minutes, 8 layers of filtered through gauze, are concentrated to density 1.10 (measure when temperature 60 C and obtain), obtain Rhizoma Polygonati Odorati extract.
(2) by the ratio mixing of dried jasmine flower and deionized water 1:10 in mass ratio, infiltrate 4h in 70 DEG C, 8 layers of filtered through gauze, obtain filtrate.
(3) by above-mentioned Rhizoma Polygonati Odorati extract and Flos Jasmini Sambac extract in mass ratio 15:1 mix, stir, through 0.45 μm of filtering with microporous membrane, through 121 DEG C of sterilizings 20 minutes, naturally cool to room temperature.
(4) will the cultured youth of shaking table must be inoculated in yeast complete medium (YPDA culture medium) by candida mycoderma (Candidalambica) CGMCC2.1763 in advance, add the mixture that step (3) obtains again, add that volume is equivalent to yeast complete medium volume 1/5 of mixture, makes yeast concentration be 8 × 10
6cell/ml, ferments, and fermentation parameter is pH value is 4.0 ~ 7.0, and mixing speed is 60 ~ 250rpm, and ventilation is 1 ~ 4VVM, and fermentation temperature is 25 DEG C ~ 30 DEG C, and fermentation time is 4 days.
(5) the Preliminary fermentation liquid butterfly seperator obtained after fermentation is separated, collects separation of supernatant, through 0.2 μm of filtering with microporous membrane, obtain yeast fermentation compound.
Embodiment 5
(1) Rhizoma Polygonati Odorati is cleaned, pulverized 60 mesh sieves, add the deionized water being equivalent to Rhizoma Polygonati Odorati 6 times of quality, 60 DEG C ultrasonic (300W) extract 30 minutes, then heated and boiled extracts 30 minutes, 8 layers of filtered through gauze, are concentrated to density 1.08 (measure when temperature 60 C and obtain), obtain Rhizoma Polygonati Odorati extract.
(2) by the ratio mixing of dried jasmine flower and deionized water 1:8 in mass ratio, infiltrate 2h in 80 DEG C, 8 layers of filtered through gauze, obtain filtrate.
(3) by above-mentioned Rhizoma Polygonati Odorati extract and Flos Jasmini Sambac extract in mass ratio 20:1 mix, stir, through 0.45 μm of filtering with microporous membrane, through 115 DEG C of sterilizings 10 minutes, naturally cool to room temperature.
(4) will be inoculated in yeast complete medium (YPDA culture medium) by the cultured saccharomyces cerevisiae of shaking table (Saccharomycescerevisiae) CGMCC2.1 in advance, add the mixture that step (3) obtains again, add that volume is equivalent to yeast complete medium volume 30% of mixture, makes yeast concentration be 6 × 10
6cell/ml, ferments, and fermentation parameter is pH value is 4.0 ~ 7.0, and mixing speed is 60 ~ 250rpm, and ventilation is 1 ~ 4VVM, and fermentation temperature is 25 DEG C ~ 30 DEG C, and fermentation time is 2 days.
(5) the Preliminary fermentation liquid butterfly seperator obtained after fermentation is separated, collects separation of supernatant, through 0.2 μm of filtering with microporous membrane, obtain yeast fermentation compound.
Reference examples 1
(1) ratio of dried jasmine flower and deionized water 1:10 is in mass ratio infiltrated 2h in 80 DEG C, 8 layers of filtered through gauze, obtain filtrate.
(2) will be inoculated in yeast complete medium by the cultured saccharomyces cerevisiae of shaking table (Saccharomycescerevisiae) CGMCC2.1 in advance, add the mixture that step (3) obtains again, add that volume is equivalent to yeast complete medium volume 1/10 of mixture, makes yeast concentration be 6 × 10
6cell/ml, ferments, and fermentation parameter is pH value is 4.0 ~ 7.0, and mixing speed is 60 ~ 250rpm, and ventilation is 1 ~ 4VVM, and fermentation temperature is 25 DEG C ~ 30 DEG C, and fermentation time is 2 days.
(3) the Preliminary fermentation liquid seperator obtained after fermentation is separated, collects separation of supernatant, through 0.2 μm of filtering with microporous membrane, obtain yeast fermentation compound.
Reference examples 2
(1) will the cultured youth of shaking table must be inoculated in yeast complete medium by candida mycoderma (Candidalambica) CGMCC2.1763 in advance, and make yeast concentration be 6 × 10
6cell/ml, ferments, and fermentation parameter is pH value is 4.0 ~ 7.0, and mixing speed is 60 ~ 250rpm, and ventilation is 1 ~ 4VVM, and fermentation temperature is 25 DEG C ~ 30 DEG C, and fermentation time is 2 days.
(2) the Preliminary fermentation liquid seperator obtained after fermentation is separated, collects separation of supernatant, through 0.2 μm of filtering with microporous membrane, obtain yeast fermentation liquor.
Reference examples 3
(1) Rhizoma Polygonati Odorati is cleaned, pulverized 60 mesh sieves, add the deionized water being equivalent to Rhizoma Polygonati Odorati 5 times of quality, 50 DEG C ultrasonic (120W) extract 30 minutes, then heated and boiled extracts 60 minutes, 8 layers of filtered through gauze, are concentrated to density 1.08 (measure when temperature 60 C and obtain), obtain Rhizoma Polygonati Odorati extract.
(2) by the ratio mixing of dried jasmine flower and deionized water 1:8 in mass ratio, infiltrate in 80 DEG C and extract 2h, 8 layers of filtered through gauze, obtain filtrate.
(3) by above-mentioned Rhizoma Polygonati Odorati extract and Flos Jasmini Sambac extract in mass ratio 1:1 mix, stir, through 0.45 μm of filtering with microporous membrane, through 121 DEG C of sterilizings 20 minutes, naturally cool to room temperature, obtain Rhizoma Polygonati Odorati extract and Flos Jasmini Sambac extract mixed liquor.
(4) will be inoculated in yeast complete medium (YPDA culture medium) by the cultured saccharomyces cerevisiae of shaking table (Saccharomycescerevisiae) CGMCC2.1 in advance, and make yeast concentration be 8 × 10
6cell/ml, ferments, and fermentation parameter is pH value is 4.0 ~ 7.0, and mixing speed is 60 ~ 250rpm, and ventilation is 1 ~ 4VVM, and fermentation temperature is 25 DEG C ~ 30 DEG C, and fermentation time is 3 days.
(5) the Preliminary fermentation liquid seperator obtained after fermentation is separated, collects separation of supernatant, through 0.2 μm of filtering with microporous membrane, obtain fermentation liquid stock solution.
(6) by Rhizoma Polygonati Odorati extract in step (3) and Flos Jasmini Sambac extract mixed liquor, add in the fermenation raw liquid that step (5) obtains again, in step (3) mixed liquor add that volume is equivalent to the fermenation raw liquid volume that step (5) obtains 1/10, obtain yeast mixture compound.
Effect example:
(1) detection of whitening effect: tyrosinase inhibition test: test philosophy: tryrosinase carries out catalytic reaction to DOPA, DOPA quinone can be generated, DOPA quinone maximum absorption wavelength is at 475nm, by measuring the absorptance of 475nm wavelength, the size of the concentration generating DOPA quinone can be reflected.When tryrosinase and whitening active ingredients are deposited in the solution simultaneously, the catalytic activity of tryrosinase by suppression to a certain extent, thus can reduce the generation of DOPA quinone.According to the contrast of front and back absorbance, the suppression degree of whitening active ingredients to tyrosinase activity can be judged, thus assess this kind of whitening composition white-skinned face function in vitro.
Reagent: (phosphate standard buffer solution takes the potassium dihydrogen phosphate (KH after 110 DEG C ~ 130 DEG C drying 2 ~ 3h to reagent 1:PBS respectively
2pO
4) 3.388g and sodium hydrogen phosphate (Na
2hPO
4) 3.533g is water-soluble and in volumetric flask, be diluted to 1L), pH value 6.86; Reagent 2: DOPA solution (with PBS preparation, mass fraction is 0.03%); Reagent 3: tyrosinase solution (with PBS preparation, concentration 200 μ g/ml); Reagent 4: yeast fermentation compound sample solution (embodiment and reference examples, identical extension rate).
Experimental technique: A test specimens: 3mlPBS+0.5ml tyrosinase solution; B test specimens: PBS; C test specimens: 1ml sample solution+2mlPBS+0.5ml tyrosinase solution; D test specimens: 1ml yeast fermentation compound sample solution+2.5mlPBS.A, B, C, D test after sample liquid prepares simultaneously and add DOPA solution 0.5ml respectively immediately, at 25 DEG C of isothermal reaction 5min, measure the absorbance at 475nm place.Often the parallel repetition of group test 3 times, averages.
The computational methods of tyrosinase inhibition rate:
Tyrosinase inhibition rate %=[(A-B)-(C-D)]/(A-B) × 100
The measurement result of tyrosinase inhibition test, as shown in table 1:
Table 1
Suppression ratio | |
Embodiment 1 | 53.72% |
Embodiment 2 | 56.10% |
Embodiment 3 | 55.84% |
Embodiment 4 | 54.37% |
Embodiment 5 | 55.92% |
Reference examples 1 | 40.25% |
Reference examples 2 | 37.18% |
Reference examples 3 | 44.92% |
(2) detection of moistening effect: keratodermatitis hydration rate is tested: test philosophy take capacitor as instrument probe, because water is the material that on skin, dielectric constant is maximum, when skin moisture content changes, the capacitance of skin also changes, so can by measuring skin pricktest capacitance, analyzing skin surface moisture content.Conventional instrument is CorneometerCM825.By measuring the change using the change of keratodermatitis capacitance before and after product to weigh water content of stratum corneum, thus moisture-keeping efficacy this kind of method evaluating cosmetics carries out quantification to the moisture of keratodermatitis, the change of skin moisture content can be reacted delicately, and favorable reproducibility, be one of method that current moisture-keeping cosmetics efficacy assessments is conventional.
Experimental technique: bend side at candidate left and right forearm and mark 3 × 3cm
2the square Experimental Area of size, using left arm as the test zone of moisturizer, the corresponding symmetrical region of right arm is blank, then detects the moisture at each test position with CorneometerCM825 test, repeats 5 times respectively, draw meansigma methods.By formula I calculated hydration rate.
Hydration rate=(test value-blank value)/blank value × 100%
(formula I).
The testing result of moistening effect, as shown in table 2:
Table 2
Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Embodiment 5 | Reference examples 1 | Reference examples 2 | Reference examples 3 | |
1h | 79% | 81% | 73% | 78% | 79% | 66% | 62% | 67% |
2h | 65% | 60% | 66% | 59% | 63% | 52% | 47% | 55% |
4h | 47% | 51% | 49% | 47% | 48% | 46% | 43% | 48% |
6h | 43% | 46% | 43% | 42% | 44% | 43% | 40% | 43% |
8h | 39% | 41% | 38% | 40% | 40% | 39% | 38% | 40% |
Application Example
(1) cleansing milk formula (by mass percentage) as shown in table 3:
Table 3
Cleansing milk processing technology is as follows:
1. polyoxyethylenated alcohol sodium sulfate in A phase, sodium lauryl sulphate, cocoanut fatty acid diethanolamide, cocamido propyl betaine, dodecayl dimethyl amine oxide, glycerol and Methylisothiazolinone are dissolved in deionized water, are heated to 70 DEG C while stirring;
2. 70 DEG C are heated to while stirring by after lanoline in B phase and stearic acid mixing;
3. by step 2. gains slowly add step 1. in gains, 65 DEG C are stirred homogenizing and cool to 50 DEG C, added by the yeast fermentation compound in C phase, continue to stir;
4. stop stirring after temperature is down to 35 DEG C, discharging, obtains yeast fermentation compound cleansing milk.
(2) astringent formula (by mass percentage) as shown in table 4:
Table 4
Astringent processing technology is as follows:
1. 1,3 butylene glycol, Methylisothiazolinone, glycyrrhizic acid dipotassium, hyaluronate sodium, yeast fermentation compound and deionized water in B phase are mixed, are stirred to dissolving;
2. glycerol, linolenic acid, tween 80, vitamin A palmitate and Vitamin E acetate in A phase are stirred all
3. by step 2. gains join step 1. in gains, stir, filter and get final product.
(3) preparation of whitening skin and preserving moisture frost
The present invention gets above-mentioned yeast fermentation compound and is mixed with whitening skin and preserving moisture frost, formula (by mass percentage) as shown in table 5:
Table 5
The processing technology of whitening skin and preserving moisture frost is as follows:
1. in oil phase pot, add the caprylic/capric triglyceride in A phase, Jojoba oil, Butyrospermum parkii fruit fat, Cyclomethicone, glyceryl stearate, cetearyl alcohol, PEG-20 methyl semi-solid fat acid esters and methyl semi-solid fat acid esters, be heated to 85 DEG C while stirring;
2. in aqueous phase pot, add each composition in B phase, be heated to 85 DEG C while stirring;
3. in emulsifying pot, homogenizing is stirred to oil pumping phase respectively after emulsifying pot evacuation and aqueous phase;
4. after temperature is down to 65 DEG C, adds C phase triethanolamine homogenizing again, after being cooled to 45 DEG C, adds the Methylisothiazolinone in D phase, under stirring, add E phase yeast fermentation compound;
5. stop stirring after temperature is down to 36 DEG C, discharging, obtains yeast fermentation compound whitening skin and preserving moisture frost.
Respectively by yeast fermentation compound obtained moisturizing skin whitener called after whitening skin and preserving moisture frost 1, whitening skin and preserving moisture frost 2, whitening skin and preserving moisture frost 3, whitening skin and preserving moisture frost 4, the whitening skin and preserving moisture frost 5 respectively as stated above of embodiment 1 ~ 5.
The above-mentioned moisturizing skin whitener prepared is carried out clinic trial observation.
1. volunteer's condition:
(1) women, the age (trimester of pregnancy or women breast-feeding their children except) between 18 ~ 60 years old; Face pigmentation in various degree, problem skin 35 example such as dark yellow, dry.
(2) without serious systemic disease, without immunodeficiency or autoimmune disease person, recipient site do not accept skin treating, beauty treatment and other may affect the test of result;
(3) without activeness anaphylactic disease person;
(4) without the extremely sensitive person of body constitution;
(5) hormone medicine and immunosuppressant person was not used in nearly one month;
(6) present or nearest three months recipient site do not participate in other clinical experiment persons.
Observational technique: before sleep every night after cleaning skin, coats above-mentioned whitening skin and preserving moisture frost.Continuous use 35 days is a course for the treatment of.Criterion of therapeutical effect: (1) is effective: dark yellow the disappearing of pigment reaches more than 90%, skin has obvious whitening to moisten smooth feeling; (2) effective: pigment is dark yellow disappears more than 50%, and skin is moistened smooth compared with whitening; (3) invalid: unchanged before and after treatment.Result is as shown in table 6:
Table 6
Effective | Effectively | Invalid | Total effective rate | |
Whitening skin and preserving moisture frost 1 | 29 | 1 | 5 | 85.71% |
Whitening skin and preserving moisture frost 2 | 27 | 2 | 6 | 82.86% |
Whitening skin and preserving moisture frost 3 | 28 | 3 | 4 | 88.57% |
Whitening skin and preserving moisture frost 4 | 27 | 4 | 4 | 88.57% |
Whitening skin and preserving moisture frost 5 | 28 | 4 | 3 | 91.43% |
There is no zest or the allergic symptom such as pruritus, erythema feedback in 35 examples (often group) under observation or describe.
Above-described embodiment is the present invention's preferably embodiment; but embodiments of the present invention are not restricted to the described embodiments; change, the modification done under other any does not deviate from spirit of the present invention and principle, substitute, combine, simplify; all should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (10)
1. a yeast fermentation compound, is characterized in that: be add in yeast culture medium by Rhizoma Polygonati Odorati extract and Flos Jasmini Sambac extract, the fermentation liquid obtained after fermenting with yeast.
2. yeast fermentation compound according to claim 1, is characterized in that:
Described Rhizoma Polygonati Odorati extract is water extract;
Described Flos Jasmini Sambac extract is water extract;
Described yeast culture medium is the culture medium of applicable Yeast Growth breeding;
The condition of described fermentation is the condition of applicable Yeast Growth breeding.
3. yeast fermentation compound according to claim 2, is characterized in that:
Described Rhizoma Polygonati Odorati extract is for prepare as follows: by Fragrant Solomonseal Rhizome and water mixing, supersound extraction, and then heated and boiled is extracted, concentrated, obtains Rhizoma Polygonati Odorati extract;
Described Flos Jasmini Sambac extract is for prepare as follows: by dried jasmine flower and water mixing, heating infiltrates, and filters, obtains Flos Jasmini Sambac extract.
4. yeast fermentation compound according to claim 1, is characterized in that:
Described Rhizoma Polygonati Odorati extract and described Flos Jasmini Sambac extract 0.01:1 ~ 100:1 proportioning in mass ratio;
Described yeast for youth must one in candida mycoderma (Candidalambica) CGMCC2.1763 and saccharomyces cerevisiae (Saccharomycescerevisiae) CGMCC2.1 or two kinds;
The condition of described fermentation is as follows: pH value is 4.0 ~ 7.0, and mixing speed is 60 ~ 250rpm, and ventilation is 1 ~ 4VVM, and fermentation temperature is 25 DEG C ~ 30 DEG C, and fermentation time is 2 days ~ 4 days.
5. the preparation method of the yeast fermentation compound described in any one of Claims 1 to 4, is characterized in that comprising following steps:
(1) by Fragrant Solomonseal Rhizome and water mixing, supersound extraction, then heated and boiled is extracted, concentrated, obtains Rhizoma Polygonati Odorati extract;
(2) by dried jasmine flower and water mixing, heating infiltrates, and filters, obtains Flos Jasmini Sambac extract;
(3) above-mentioned Rhizoma Polygonati Odorati extract is mixed with Flos Jasmini Sambac extract, stir, filter, degerming, obtain mixture; Mixture is mixed with the yeast culture medium through bacteria removing, obtains fermentation medium;
(4) yeast is inoculated in fermentation medium ferments;
(5) the Preliminary fermentation liquid obtained after fermentation is carried out solid-liquid separation, get liquid, obtain yeast fermentation compound.
6. the preparation method of yeast fermentation compound according to claim 5, is characterized in that:
The condition of the supersound extraction described in step (1) is in 50 ~ 60 DEG C, 100 ~ 500W supersound extraction 30 ~ 60 minutes;
The time that heated and boiled described in step (1) is extracted is 30 ~ 90 minutes;
Concentrated degree described in step (1) is for being concentrated to density 1.01 ~ 1.20;
The consumption of the water described in step (2) is for being equivalent to dried jasmine flower quality 5 ~ 10 times;
The condition that heating described in step (2) infiltrates is infiltrate 0.5 ~ 4h in 70 DEG C ~ 100 DEG C;
Rhizoma Polygonati Odorati extract described in step (3) and described Flos Jasmini Sambac extract 0.01:1 ~ 100:1 proportioning in mass ratio;
Add that volume is described yeast culture medium volume 10 ~ 20% of mixture described in step (3).
7. the preparation method of yeast fermentation compound according to claim 6, is characterized in that:
Described ultrasonic power is in 50 ~ 60 DEG C, 100 ~ 300W supersound extraction 30 ~ 60 minutes;
The condition that described heating infiltrates is infiltrate 2 ~ 4h in 70 DEG C ~ 90 DEG C;
Described Rhizoma Polygonati Odorati extract and described Flos Jasmini Sambac extract 1:1 ~ 20:1 proportioning in mass ratio;
Described yeast culture medium is YPDA culture medium.
8. the yeast fermentation compound described in any one of Claims 1 to 4 is preparing the application in whitening skin and preserving moisture skin care item.
9. yeast fermentation compound according to claim 8 is preparing the application in whitening skin and preserving moisture skin care item, it is characterized in that: the concentration of described yeast fermentation compound in described whitening skin and preserving moisture skin care item is mass percent 1 ~ 30%.
10. whitening skin and preserving moisture skin care item, is characterized in that: containing the yeast fermentation compound described in any one of Claims 1 to 4.
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