CN105434319B - A kind of cosmetics and preparation method thereof based on phaffiafhodozyma raw material - Google Patents
A kind of cosmetics and preparation method thereof based on phaffiafhodozyma raw material Download PDFInfo
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- CN105434319B CN105434319B CN201510896328.0A CN201510896328A CN105434319B CN 105434319 B CN105434319 B CN 105434319B CN 201510896328 A CN201510896328 A CN 201510896328A CN 105434319 B CN105434319 B CN 105434319B
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- phaffiafhodozyma
- raw material
- astaxanthin
- yeast
- cosmetics
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- 229910021642 ultra pure water Inorganic materials 0.000 description 1
- 239000012498 ultrapure water Substances 0.000 description 1
- 230000029663 wound healing Effects 0.000 description 1
- 230000037303 wrinkles Effects 0.000 description 1
- 210000005253 yeast cell Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/31—Hydrocarbons
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/35—Ketones, e.g. benzophenone
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
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- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Dermatology (AREA)
- Emergency Medicine (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses a kind of cosmetics and preparation method thereof based on phaffiafhodozyma raw material.The cosmetics contain the astaxanthin from phaffiafhodozyma, or containing the astaxanthin from phaffiafhodozyma, the glucan from phaffiafhodozyma and from the yeast extract of phaffiafhodozyma.Raw material used in the present invention comes from phaffiafhodozyma, safe;Moreover, astaxanthin, glucan and the yeast extract collocation from phaffiafhodozyma use, the free radical scavenging ability of cosmetics can be further enhanced.The present invention is utilized to the deep development of phaffiafhodozyma, and the Economic Application value for improving phaffiafhodozyma is conducive to.
Description
Technical field
The present invention relates to cosmetic fields, more particularly to a kind of cosmetics based on phaffiafhodozyma raw material and its preparation
Method.
Background technology
Free radical is the effective system of defense of body, but free radical generates excessive, can attack life macromolecule substance and each
Kind organelle, can cause body in the various damages of molecular level, cellular level and histoorgan level, accelerate the aging of body
Process simultaneously induces various diseases.Free radical causes Skin Cell the damage of unrepairable, leads to skin aging;Free radical is to thin
The destruction of after birth lipid is lipofuscin precipitation, makes skin spots;Damage of the free radical to nuclear of epidermal cells can induce skin
Cancer.The environment that human survival has free radical everywhere in one is constantly injured by external freedom base, while mankind itself's body
It inside also constantly generates free radicals, accelerates the aging death of human body.Therefore, effective radicals scavenging is to pass for the mankind
Important.
Phaffiafhodozyma (Phaffiarhodozym), a kind of micro- life of obligate oxygen consumption that can synthesize a variety of carotenoid
Object.Wild phaffiafhodozyma can produce more than 10 kinds of carotenoid, wherein mainly astaxanthin.Thus, phaffiafhodozyma is production day
The bacterial strain that right astaxanthin generally uses.Due to the total nitrogen content about 48.2% of the primary yeast, protein content about 30.1%, total reducing sugar
Content about 40.3%, therefore can be also as the good raw material for preparing yeast extract and glucan, to make phaffiafhodozyma more
It is adequately exploited, further increases economic benefit.
Astaxanthin (Astaxanthin), 3,3- dihydroxy -4,4- diketo-β, beta carotene, molecular formula C40H52O4, phase
Not soluble in water to molecular weight 596.86, tool is fat-soluble, is a kind of terpenes unsaturated compounds, is widely present in living nature.
Due to the unsaturated ketone group and hydroxyl of conjugated double bond and conjugated double bond end in astaxanthin molecular structure, can attract freely
Base is to have extremely strong antioxidation.Many histoorgans such as skin, physiological function is because being often exposed to free radical
A large amount of free radicals are generated in environment or in metabolic process, are encroached on by free radical.Astaxanthin is fat-soluble good, has to cell membrane
Affinity easily propagates through blood barrier and reaches each histoorgan, can effectively remove the freedom generated in vivo by ultraviolet light irradiation
Base, adjusting reduces these is injured caused by photochemistry.Astaxanthin is a kind of highly effective bright protective agent, can be in skin
Putrescine is consumed when by light, can be used for preventing the skin aging caused by light radiation and cutaneum carcinoma.Astaxanthin cosmetics are developed, not only
Light radiation can be prevented, also has the effects that anti-cell aging.
Yeast extract (Yeast extract), be a kind of natural, nutrition, health yeast deep-processed food dispensing, by
The higher active ingredients composition of various nutritive values, including the polypeptide and amino acid, raw after nucleolysis that are generated after protein degradation
At nucleotide and B family vitamin, trace element etc..Wherein 30% or more amino acid content, 50% or more total protein, nucleosides
10% or more acid, it is living and other effects with moisturizing, tax, it is widely used in cosmetic field.Amino acid is natural in keratoderma
The main component of moisturizing factor (NMF), is easily absorbed by the skin, and the epidermis of aging is made to restore elasticity, delay skin aging.Nucleic acid and
Nucleotide is the central genetic substance of human body, has the effects that the protein aggregate velocity that boosts metabolism, improves, enhancing are immune
Function, enhancement of SOD activity, improve skin antiradical activities.In addition, the nutrition member such as vitamin and mineral a small amount of in product
Element can also provide sufficient nutrition for skin.
Glucan (Glucan) is a kind of glucose polymer extracted from yeast cell wall, because it has enhancing immune
Function is known as being biological effect response agent.Currently, glucan has realized industrialization, products application is in body-care food and moves
The immunoactivator of object feed.Research also found that yeast dextran can accelerate skin wound healing, have higher free radical clear
Removing solid capacity has fine performance of keeping humidity and crease-resistant, anti-senescence function to skin, there is good application prospect in terms of skin care item.Just
Step is studies have shown that phaffiafhodozyma cell wall alkali-insoluble glucan content is higher.
With the improvement of living standards, the demand that people are especially cosmetics in the cosmetics with given efficacy increasingly increases
Add.Pure natural raw material due to the use of safety, action temperature and it is with obvious effects the advantages that, increasingly obtain cosmetic Manufacture business and
The favor of consumer.Currently, effect ingredient in most of cosmetics is mainly chemical synthesis, and remove the makeup of free radical
Its radicals scavenging effect unobvious of product, and easily cause allergy.Therefore, cosmetics are prepared using phaffiafhodozyma raw material, more
Add safely and effectively.And pass through three kinds of astaxanthin, yeast extract and glucan raw materials for adding phaffiafhodozyma source simultaneously, tool
It has the advantage that:First, the function of phaffiafhodozyma can be made to be fully developed, second is that the inoxidizability of product can be enhanced,
Third, same oxidation resistant product, can reduce the addition of astaxanthin by additionally adding yeast extract and glucan, from
And reduce production cost.
There is no research report simultaneously using three kinds of astaxanthin in phaffiafhodozyma source, yeast extract and glucan raw materials come
Prepare the cosmetics for effectively removing free radical.
Invention content
The primary purpose of the present invention is that the shortcomings that overcoming the prior art and deficiency, provide a kind of former based on phaffiafhodozyma
The cosmetics of material.
Another object of the present invention is to provide the preparation methods of the cosmetics based on phaffiafhodozyma raw material.
The purpose of the invention is achieved by the following technical solution:A kind of cosmetics based on phaffiafhodozyma raw material, contain
From the astaxanthin of phaffiafhodozyma, or containing from phaffiafhodozyma astaxanthin, from phaffiafhodozyma
Glucan and yeast extract from phaffiafhodozyma.
The astaxanthin from phaffiafhodozyma, the glucan from phaffiafhodozyma and derive from red hair husband
The yeast extract of yeast is preferably made by the steps to obtain:
(1) pretreatment of cell:The yeast thalline for weighing phaffiafhodozyma is made into yeast bacteria suspension by certain solid-to-liquid ratio,
A certain amount of cellulase is added, mixing, it is 4~5 to adjust pH value, 4~12h of enzyme digestion reaction at 45~55 DEG C;After reaction,
Enzyme deactivation is lived, and is separated by solid-liquid separation, is taken precipitation, and precipitation is cleaned, pretreated yeast thalline is obtained;
(2) Astaxanthin extraction:Organic solvent is added in the pretreated yeast thalline that step (1) obtains, is protected from light oscillation
Extraction is separated by solid-liquid separation, and liquid is yeast pigment extract, and main component therein is astaxanthin;Solid is used to prepare yeast pumping
Extract;
(3) preparation of yeast extract:After the solid mixing that water and step (2) are obtained, it is subsequently added into extraction from yeast enzyme
And inorganic salts, in 55 DEG C of reactions, enzyme deactivation after reaction is lived, is separated by solid-liquid separation, and is yeast extract after liquid drying, and solid is used
In preparing glucan;
(4) prepared by glucan:The solid mixing that lye and step (3) are obtained is reacted, and is separated by solid-liquid separation, solid is cleaned
And it is dry to get to yeast dextran.
Red phaffia rhodozyma described in step (1) is preferably red 2.1557 bacterial strains of phaffia rhodozyma As or red phaffia rhodozyma
ATCC66270 bacterial strains.
Yeast thalline described in step (1) is the yeast thalline or wet yeast thalline by being dried.
The yeast thalline containing 10~20g in the preferably every 100mL reaction systems of the system of enzyme digestion reaction described in step (1)
With 0.5~6mL cellulase solutions.
Cellulase solution described in step (1) is cellulase crude enzyme liquid.
PH value described in step (1) is adjusted preferably by hydrochloric acid;More preferably adjusted by the hydrochloric acid of a concentration of 3M.
The temperature of enzyme digestion reaction described in step (1) is preferably 50 DEG C.
Organic solvent described in step (2) is preferably edible ethanol.
The dosage of organic solvent described in step (2) is preferably organic molten by 40~80mL is matched per g yeast dry myceliums
Agent calculates;It more preferably presses and is calculated per g yeast dry mycelium proportioning 60mL organic solvents.
Yeast pigment extract described in step (2) not only contains astaxanthin, also contains a small amount of lycopene and β-
Carrotene, using astaxanthin as main component.
The time of extraction described in step (2) is preferably 20~90min;More preferably 60~70min.
Water described in step (3) is preferably deionized water or ultra-pure water.
The dosage of water described in step (3) is preferably to be calculated by the solid proportioning 100ml water obtained per 10g steps (2).
The additive amount of extraction from yeast enzyme described in step (3) preferably matches 0.01~0.03g by per g yeast dry myceliums
Extraction from yeast enzyme calculates.
Inorganic salts described in step (3) are preferably potassium chloride.
The additive amount of inorganic salts described in step (3) is preferably inorganic by 0.01~0.03g is matched per g yeast dry myceliums
Salt calculates.
The mode of drying described in step (3) and step (4) includes that freeze-drying, spray drying or rotary evaporation are dry
It is dry.
Lye described in step (4) is preferably sodium hydroxide solution;More preferably a concentration of mass percent 2-6%'s
Sodium hydroxide solution.
The dosage of lye described in step (4) matches 100ml lye by the solid that every 12.5~50g steps (3) obtain
It calculates.
The condition of reaction described in step (4) is preferably 45~75 DEG C of 1~3h of reaction.
The method of separation of solid and liquid described in above-mentioned steps is centrifugal process or filtration method.
The condition that enzyme deactivation described in step (1) and step (3) is lived preferably handles 3~5min in 95~100 DEG C.
The astaxanthin from phaffiafhodozyma it is described based on the cosmetics of phaffiafhodozyma raw material in contain
Amount is preferably mass percent 0.002~0.004%.
The glucan from phaffiafhodozyma is described based in the cosmetics of phaffiafhodozyma raw material
Content is preferably mass percent 0.05~5%.
The yeast extract from phaffiafhodozyma is in the cosmetics based on phaffiafhodozyma raw material
In content be preferably mass percent 0.5~10%.
The cosmetics are preferably facial mask, Essence.
When the cosmetics are facial mask, preferably by as follows by mass percentage at being grouped as:
A phases:Purified Water 30%, emulsifier 1.5%, 1,3 butylene glycol 3%, glycerin polyether -263%, EDETATE SODIUM
0.05%;
B phases:Parahydroxyacet-ophenone 0.6%, glycine betaine 2%;
C phases:1,2- hexylene glycols 0.6%, astaxanthin-lipid complex 3.6~5.4%, the water work factor 1%, stimulation inhibit
The factor 1%, yeast extract 0~0.5%, beta glucan 0~0.05%, appropriate essence;
D phases:Refined water surplus.
The facial mask is preferably made by the steps to obtain:
(1) agitated kettle is added in the raw material in A phases, heating stirring makes each component be sufficiently uniformly dissolved to 80~85 DEG C, cold
But to 60 DEG C;
(2) agitated kettle is added in the raw material in B phases when 60 DEG C, is completely dissolved postcooling to 45 DEG C;
(3) raw material in C phases is sequentially added into agitated kettle at 45 DEG C, stirred evenly;
(4) the raw material mixing in D phases is then added, obtains facial mask.
When the cosmetics are Essence, preferably by as follows by mass percentage at being grouped as:
A ' phases:Purified Water 30%, emulsifier 1%, glycerin polyether -262%, EDETATE SODIUM 0.05%;
B ' phases:1,2- hexylene glycols 0.6%, preservative 0.3%, Sodium Hyaluronate 0.2%, hyaluronic acid 1%, astaxanthin-
Lipid complex 3.6~5.4%, stimulation inhibiting factor 1%, yeast extract 0~0.5, beta glucan 0~0.05, compound ammonia
Base acid 2%, appropriate essence, moisture retention water 2%;
C ' phases:Refined water surplus.
The Essence is preferably made by the steps to obtain:
(1) agitated kettle being added in the raw material in A ' phases, heating stirring makes each component be sufficiently uniformly dissolved to 80~85 DEG C,
It is cooled to 60 DEG C;
(2) agitated kettle is added in the raw material in B ' phases when 60 DEG C, is completely dissolved postcooling to 45 DEG C;
(3) raw material in C ' phases is sequentially added into agitated kettle at 45 DEG C, stirs evenly, obtains Essence.
Astaxanthin-the lipid complex, is made by the steps to obtain:Full of inert gas and dark surrounds
Middle to be mixed with grease from the astaxanthin of phaffiafhodozyma, centrifugation takes liquid, obtains astaxanthin-lipid complex.
The grease be preferably caprylic/capric triglyceride, isononyl isononanoate, isopropyl myristate, olive oil,
One kind in saualane, Sweet Almond Oil and glycerine or at least two.
The emulsifier is preferably Lecigel emulsifiers.
The water work factor is preferably the AQUAXYL water work factor.
The stimulation inhibiting factor is preferably BioAegis stimulation inhibiting factors.
The preparation method of the cosmetics based on phaffiafhodozyma raw material, includes the following steps:
(1) it will be mixed with grease from the astaxanthin of phaffiafhodozyma in full of inert gas and dark surrounds, from
The heart takes liquid, obtains astaxanthin-lipid complex;
(2) astaxanthin-lipid complex is added in cosmetics, mixing, obtains the change based on phaffiafhodozyma raw material
Cosmetic;Or by astaxanthin-lipid complex, from phaffiafhodozyma yeast extract and derive from phaffiafhodozyma
Glucan be added in cosmetics, mixing obtains the cosmetics based on phaffiafhodozyma raw material.
Inert gas described in step (1) is preferably nitrogen.
Grease described in step (1) is preferably caprylic/capric triglyceride, isononyl isononanoate, myristic acid isopropyl
One kind in ester, olive oil, saualane, Sweet Almond Oil and glycerine or at least two.
Mixing described in step (1) is preferably by high speed high-shear impeller, high pressure homogenizer or ultrasonic refiner
It is mixed.
The additive amount of astaxanthin-lipid complex described in step (2) is according to pure astaxanthin additive amount in cosmetics
0.002~0.004% is added.
The temperature environment of mixing described in step (2) is preferably 40~50 DEG C;More preferably 45 DEG C.
Mixing described in step (2) is preferably by high speed high-shear impeller, high pressure homogenizer or ultrasonic refiner
It is uniformly mixed.
Effect ingredient in most of cosmetics in the market is mainly chemical synthesis at present, anti-to easy to produce allergy
It answers.Relatively fewer about the product for removing free radical in the market, there are certain gaps in market.For most of market-oriented
Removing free radical cosmetics, radicals scavenging effect unobvious, the ability for resisting skin aging is poor, cannot be satisfied people
The demand that slows down aging, retain youth.Therefore there is an urgent need for develop the stronger raw material of natural free radical scavenging ability and answer
It uses in cosmetics.The present invention is added to cosmetic formulations by preparing the astaxanthin compound containing phaffiafhodozyma source
In, while the yeast extract and beta glucan in addition phaffiafhodozyma source are clear to prepare stronger free radical in cosmetic formulations
The cosmetics of removing solid capacity, its advantage is that:
(1) raw material used in the present invention comes from phaffiafhodozyma.Red phaffia rhodozyma is that the edible of the safety of FDA certifications is micro-
Biology is to produce the major microorganisms of natural astaxanthin, while phaffiafhodozyma is also used to prepare yeast extract and β-Portugal
Glycan.Therefore, the raw material (including astaxanthin, yeast extract and glucan) in phaffiafhodozyma source is all pure natural, safety
Property it is high.
(2) present invention used in phaffiafhodozyma source astaxanthin and yeast in other carotenoid as a mixture
In the presence of wherein main component is astaxanthin, while also containing some lycopenes and beta carotene.Astaxanthin be it is anti-oxidant it
King has extremely strong free radical scavenging ability.Contained lycopene and beta carotene also has certain radicals scavenging
Ability can prevent the generation of the lipid peroxide in skin surface and skin, to prevent skin occur inflammation, it is dimmed,
The aging phenomena such as wrinkle and relaxation.
(3) present invention adds the yeast extract and beta glucan in phaffiafhodozyma source in cosmetic formulations for the first time, this
Two kinds of raw materials also have certain free radical scavenging ability.By adding both raw materials, increase can further be cooperateed with to make up
The free radical scavenging ability of product.
(4) astaxanthin, three kinds of raw materials of glucan and yeast extract of the invention by adding phaffiafhodozyma source simultaneously,
The function of phaffiafhodozyma can be made to be fully developed, while the beta glucan used in the present invention is by the thick enzyme of cellulase
What liquid and protease hydrolytic obtained, it is water-soluble higher, it no longer needs to that by other modifications production can be applied directly to
In.
(5) astaxanthin, three kinds of raw materials of glucan and yeast extract of the invention by adding phaffiafhodozyma source simultaneously,
For same oxidation resistant product, the addition of astaxanthin can be reduced by additionally adding yeast extract and beta glucan,
To reduce the production cost of product.
Specific implementation mode
With reference to embodiment, the present invention is described in further detail, and embodiments of the present invention are not limited thereto.
Embodiment 1
(1) reaction system volume is 1L, contains red phaffia rhodozyma (ATCC66270) thalline 100g and 60mL cellulase, is used
3M salt acid for adjusting pH value is about 4.5, deionized water constant volume;6h is reacted in 50 DEG C under the mixing speed of 150rpm, boils 3min
Enzyme deactivation is lived.
(2) after reaction, 10000rpm centrifuges 10min, and washing of precipitate is clean.
(3) edible ethanol 60mL/g dry myceliums are added into yeast after enzymolysis, is vibrated on the shaking table that rotating speed is 150rpm
It is protected from light extraction 60min, 10000rpm and centrifuges 10min, collect supernatant, obtain Astaxanthin extraction rate 91.93% and (pass through spectrophotometric
Meter method measures content astaxanthin, and the astaxanthin amount with acid-heat treating process extraction is control, that is, gives tacit consent to the astaxanthin of hot acid method's extraction
For whole astaxanthin amounts, recovery rate is that the astaxanthin amount of this method extraction accounts for the percentage of whole astaxanthins).
(4) according to solid-to-liquid ratio 10% add deionized water to extraction pigment after bacterial sediment in, addition 3% potassium chloride and
3% extraction from yeast enzyme under the mixing speed of 150rpm for 24 hours in 55 DEG C of reactions boils 3min enzyme deactivations work.
(5) after reaction, 10000rpm centrifuges 10min, collects supernatant and drying, obtains red phaffia rhodozyma extracting
Object measures ammonia nitrogen yield 3.9% (the ammonia nitrogen yield that yeast extract is measured by formol titration).
(6) washing of precipitate obtained after extracting is clean, and a concentration of mass percent 2% is added respectively by 50% solid-to-liquid ratio
Sodium hydroxide solution, in 45 DEG C handle 1h, 10000rpm centrifuge 10min, washing of precipitate is dried, 14.1g glucans are obtained
(glucan amount is measured by phend-sulphuric acid).
The screening of the arbitrary component of 2 astaxanthin compound of embodiment
(1) 20g caprylic/capric triglycerides, 20g isononyl isononanoates, 20g isopropyl myristates, 20g olives are taken respectively
Olive oil, 20g saualanes, 20g Sweet Almond Oils and 20g glycerine.
(2) carotenoid that will be prepared by embodiment 1 for 6% (mass fraction, i.e. 1.2g) with the amount of astaxanthin
It is added in each oil in step (1), it is mixed by high speed, high-shear impeller in room temperature (25 DEG C) nitrogen stream dark situation
It is even.
(3) 5000rpm centrifuges 10min, is separated by solid-liquid separation, takes liquid, obtains the compound of astaxanthin.
(4) measure each astaxanthin-lipid complex by spectrophotometry determines each oil to color in the absorbance of 474nm
The solvability of element.Wherein caprylic/capric triglyceride is maximum to the dissolving of pigment, OD474=292.35;Followed by nutmeg
Isopropyl propionate, OD474=57.4;Followed by isononyl isononanoate, OD474=19.52;Remaining oil is less to the dissolving of pigment,
OD474Respectively less than 15.
(5) content astaxanthin of astaxanthin-lipid complex is measured by efficient liquid phase.Wherein astaxanthin-caprylic/capric
The amount highest of astaxanthin in triglycerides compound is 539.4mg/L, and it is 0.056% to be equivalent to mass fraction;Followed by shrimp is green
Element-isopropyl myristate compound, content astaxanthin 128.4mg/L, it is 0.015% to be equivalent to mass fraction;Followed by
Astaxanthin-isononyl isononanoate compound, content astaxanthin 31.7mg/L are equivalent to 0.004%;Remaining oil prawn is green
The solubility of element is too small, is less than 0.0004%.
The DPPH free radical scavenging abilities of 3 astaxanthins of embodiment-caprylic/capric triglyceride compound determine
(1) astaxanthin of 539.4mg/L-caprylic/capric triglyceride compound mother liquor is diluted to shrimp with absolute ethyl alcohol
Green cellulose content be 5.4,7.2,9.6,12.8,17.1,22.76,30.34,40.46,53.94mg/L.
(2) the DPPH free radical abilities of astaxanthin compound under each concentration are determined with removing organic free radical DPPH methods
(Anarjan N,et al.Colloidal astaxanthin:Preparation,characterisation and
bioavailability evaluation,Food Chem.2012,135(3):1303-1309.).It obtains in the concentration range
The content of interior astaxanthin and its DPPH free radical scavenging activity are linear, and relational expression is:Y=1.397x+5.585, R2=
0.992, wherein Y are DPPH free radical scavenging activities (%), and X is astaxanthin concentration (mg/L).It can thus be appreciated that astaxanthin-octanoic acid/last of the ten Heavenly stems
The IC of acid glycerol three ester compound mother liquor50=31.8mg/L, while 22mg/L astaxanthins-caprylic/capric triglyceride compound
DPPH free radical scavenging activities be 36.5%.
The preparation of the facial mask of 4 astaxanthin-containings of embodiment-caprylic/capric triglyceride compound
The facial mask (total amount 100%) with following formulas is prepared by a conventional method:Composition of raw materials is as shown in table 1:
Table 1
Technological process:
(1) agitated kettle is added in the raw material in A phases, heating stirring makes each component be sufficiently uniformly dissolved to 80~85 DEG C, cold
But to 60 DEG C;
(2) agitated kettle is added in the raw material in B phases when 60 DEG C, is completely dissolved postcooling to 45 DEG C;
(3) raw material in C phases is sequentially added into agitated kettle at 45 DEG C, stirred evenly;
(4) the raw material mixing in D phases is then added, discharges, stands.
The facial mask for taking each concentration astaxanthin-containing-lipid complexes of 0.5g measures it with organic free radical DPPH methods are removed
DPPH free radical scavenging abilities.Wherein, the DPPH free radical scavenging activities of the facial mask of the lipid complex containing 3.6% astaxanthin-reach
34.38%;The DPPH free radical scavenging activities of the facial mask of the lipid complex containing 5.4% astaxanthin-reach 48.56%.
The facial mask of 5 astaxanthin-containings of embodiment-caprylic/capric triglyceride compound, yeast extract and beta glucan
It prepares
The facial mask (total amount 100%) with following formulas is prepared by a conventional method:Composition of raw materials is as shown in table 2:
Table 2
(1) technological process is the same as embodiment 4.
(2) facial mask for taking each concentration astaxanthin-containing compounds of 0.5g measures its DPPH with organic free radical DPPH methods are removed
Free radical scavenging ability.Wherein, the DPPH free radical scavenging activities of the facial mask of the lipid complex containing 3.6% astaxanthin-reach
50.9%;The DPPH free radical scavenging activities of the facial mask of the lipid complex containing 5.4% astaxanthin-reach 65.5%.
The preparation of 6 astaxanthin-containings of embodiment-caprylic/capric triglyceride compound Essence
The Essence (total amount 100%) with following formulas is prepared by a conventional method:Composition of raw materials is as shown in table 3:
Table 3
Technological process:
(1) agitated kettle is added in the raw material in A phases, heating stirring makes each component be sufficiently uniformly dissolved to 80~85 DEG C, cold
But to 45 DEG C;
(2) raw material in B phases is sequentially added into agitated kettle when 45 DEG C, stirred evenly;
(3) raw material in C phases is then added, stirs evenly, discharges, stand.
(4) the raw material mixing in D phases is then added, discharges, stands.
The Essence for taking each concentration astaxanthins of 0.5g-grease compounded object plane measures it with organic free radical DPPH methods are removed
DPPH free radical scavenging abilities.Wherein, the DPPH free radical scavenging activities of the Essence of the lipid complex containing 3.6% astaxanthin-reach
To 40.5%;The DPPH free radical scavenging activities of the Essence of the lipid complex containing 5.4% astaxanthin-reach 53.4%.
The preparation of the Essence of 7 astaxanthin-containings of embodiment-caprylic/capric triglyceride compound and beta glucan
The Essence (total amount 100%) with following formulas is prepared by a conventional method:Composition of raw materials is as shown in table 4:
Table 4
(1) technological process is the same as example 6.
(2) Essence for taking each concentration astaxanthins of 0.5g-grease compounded object plane is measured with organic free radical DPPH methods are removed
Its DPPH free radical scavenging ability.Wherein, the DPPH free radical scavenging activities of the Essence of the lipid complex Han 3.6% astaxanthin-
Reach 57.1%;The DPPH free radical scavenging activities of the Essence of the lipid complex containing 5.4% astaxanthin-reach 66.8%.
The DPPH free radical scavenging abilities of 1 various concentration ascorbic acid (VE) of comparative example determine
(1) by the ascorbic acid mother liquor of 268mg/L with absolute ethyl alcohol be diluted to ascorbic acid content be 4.19,8.38,
16.75、33.5、67.0、134.0mg/L。
(2) the DPPH free radical scavenging abilities of each concentration ascorbic acid are measured with removing organic free radical DPPH methods.It obtains
The content of ascorbic acid and its DPPH free radical scavenging activity are linear in the concentration range, and relational expression is:Y=0.684x
+5.529,R2=0.992, wherein Y are DPPH free radical scavenging activities (%), and X is ascorbic acid concentrations (mg/L).It can thus be appreciated that anti-
The IC50=65.02mg/L of bad hematic acid mother liquor.
Comparative example 2 contains only the DPPH free radical scavenging abilities of beta glucan and yeast extract facial mask
(1) formula for pressing embodiment 5, does not add astaxanthin-lipid complex, same process flow contains only yeast in preparation
The facial mask of extract and beta glucan.
(2) this facial mask of 0.5g is taken, its DPPH free radical scavenging ability is measured with organic free radical DPPH methods are removed.Obtain this
The DPPH free radical scavenging activities of facial mask reach 10.8%.
Comparative example 3 contains only the DPPH free radical scavenging abilities of beta glucan and yeast extract Essence
(1) formula for pressing embodiment 7, does not add astaxanthin-lipid complex, same process flow contains only yeast in preparation
The Essence of extract and beta glucan.
(2) this Essence of 0.5g is taken, its DPPH free radical scavenging ability is measured with organic free radical DPPH methods are removed.It obtains
The DPPH free radical scavenging activities of this Essence reach 12.3%.
The DPPH free radical scavenging abilities of 4 astaxanthin standard items (being purchased from Sigma) of comparative example
(1) the astaxanthin solution of a concentration of 22mg/L of astaxanthin is prepared with absolute ethyl alcohol.
(2) its DPPH free radical scavenging ability is measured with removing organic free radical DPPH methods.Obtain the DPPH of this solution certainly
Reach 28.9% by base clearance rate.
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, it is other it is any without departing from the spirit and principles of the present invention made by changes, modifications, substitutions, combinations, simplifications,
Equivalent substitute mode is should be, is included within the scope of the present invention.
Claims (10)
1. a kind of cosmetics based on phaffiafhodozyma raw material, it is characterised in that:Containing from phaffiafhodozyma astaxanthin,
Glucan from phaffiafhodozyma and the yeast extract from phaffiafhodozyma.
2. the cosmetics according to claim 1 based on phaffiafhodozyma raw material, it is characterised in that:Described derives from red hair
The astaxanthin of husband's yeast, described takes out from the glucan of phaffiafhodozyma and the yeast from phaffiafhodozyma
Extract is made by the steps to obtain:
(1) pretreatment of cell:The yeast thalline for weighing phaffiafhodozyma is made into yeast bacteria suspension by certain solid-to-liquid ratio, addition
A certain amount of cellulase, mixing, it is 4~5 to adjust pH value, 4~12h of enzyme digestion reaction at 45~55 DEG C;After reaction, enzyme deactivation
It is living, it is separated by solid-liquid separation, takes precipitation, precipitation is cleaned, pretreated yeast thalline is obtained;
(2) Astaxanthin extraction:Organic solvent is added in the pretreated yeast thalline that step (1) obtains, is protected from light mechanical shaking extraction,
It is separated by solid-liquid separation, liquid is yeast pigment extract, and main component therein is astaxanthin;Solid is used to prepare yeast extract;
(3) preparation of yeast extract:After the solid mixing that water and step (2) are obtained, it is subsequently added into extraction from yeast enzyme and nothing
Machine salt, in 55 DEG C of reactions, enzyme deactivation after reaction is lived, is separated by solid-liquid separation, and is yeast extract after liquid drying, solid is for making
Standby glucan;
(4) prepared by glucan:The solid mixing that lye and step (3) are obtained is reacted, and is separated by solid-liquid separation, solid is cleaned and done
It is dry to get to yeast dextran.
3. the cosmetics according to claim 2 based on phaffiafhodozyma raw material, it is characterised in that:Described in step (1)
Phaffiafhodozyma is 2.1557 bacterial strains of phaffiafhodozyma As or phaffiafhodozyma ATCC66270 bacterial strains.
4. the cosmetics according to claim 2 based on phaffiafhodozyma raw material, it is characterised in that:
Yeast thalline described in step (1) is the yeast thalline or wet yeast thalline by being dried;
The system of enzyme digestion reaction described in step (1) be per 100mL reaction systems in containing 10~20g yeast thalline and 0.5~
6mL cellulase solutions;
Cellulase described in step (1) is cellulase crude enzyme liquid;
The temperature of enzyme digestion reaction described in step (1) is 50 DEG C;
Organic solvent described in step (2) is edible ethanol;
The dosage of organic solvent described in step (2) is to match 40~80mL organic solvents by every g yeast dry mycelium to calculate;
The time of extraction described in step (2) is 20~90min;
The dosage of water described in step (3) is to match 100ml water by the solid that every 10g steps (2) obtain to calculate;
The additive amount of extraction from yeast enzyme described in step (3) is to match 0.01~0.03g extraction from yeast by every g yeast dry mycelium
Enzyme calculates;
Inorganic salts described in step (3) are potassium chloride;
The additive amount of inorganic salts described in step (3) is to match 0.01~0.03g inorganic salts by every g yeast dry mycelium to calculate;
Lye described in step (4) is sodium hydroxide solution;
The solid proportioning 100ml lye that the dosage of lye described in step (4) is obtained by every 12.5~50g steps (3) calculates;
The condition of reaction described in step (4) is 45~75 DEG C of 1~3h of reaction.
5. the cosmetics according to claim 1 based on phaffiafhodozyma raw material, it is characterised in that:
The astaxanthin from phaffiafhodozyma is in the content based in the cosmetics of phaffiafhodozyma raw material
For mass percent 0.002~0.004%;
The glucan from phaffiafhodozyma is in the content based in the cosmetics of phaffiafhodozyma raw material
For mass percent 0.05~5%;
The yeast extract from phaffiafhodozyma is described based in the cosmetics of phaffiafhodozyma raw material
Content is mass percent 0.5~10%.
6. according to the cosmetics based on phaffiafhodozyma raw material described in any one of Claims 1 to 5, it is characterised in that:Described
Cosmetics are facial mask or Essence.
7. the cosmetics according to claim 6 based on phaffiafhodozyma raw material, it is characterised in that:
When the cosmetics are facial mask, by as follows by mass percentage at being grouped as:
A phases:Purified Water 30%, emulsifier 1.5%, 1,3 butylene glycol 3%, glycerin polyether -26 3%, EDETATE SODIUM 0.05%;
B phases:Parahydroxyacet-ophenone 0.6%, glycine betaine 2%;
C phases:1,2- hexylene glycols 0.6%, astaxanthin-lipid complex 3.6~5.4%, the water work factor 1%, stimulation inhibiting factor
1%, yeast extract 0~0.5%, beta glucan 0~0.05%, appropriate essence;Wherein, yeast extract and beta glucan
Dosage is not 0;
D phases:Refined water surplus;
When the cosmetics are Essence, by as follows by mass percentage at being grouped as:
A ' phases:Purified Water 30%, emulsifier 1%, glycerin polyether -26 2%, EDETATE SODIUM 0.05%;
B ' phases:1,2- hexylene glycols 0.6%, preservative 0.3%, Sodium Hyaluronate 0.2%, hyaluronic acid 1%, astaxanthin-grease
Compound 3.6~5.4%, stimulation inhibiting factor 1%, yeast extract 0~0.5%, beta glucan 0~0.05%, compound ammonia
Base acid 2%, appropriate essence, moisture retention water 2%;Wherein, the dosage of yeast extract and beta glucan is not 0;
C ' phases:Refined water surplus;
Astaxanthin-the lipid complex is made by the steps to obtain:It will in full of inert gas and dark surrounds
Astaxanthin from phaffiafhodozyma is mixed with grease, and centrifugation takes liquid, obtains astaxanthin-lipid complex.
8. the cosmetics according to claim 7 based on phaffiafhodozyma raw material, it is characterised in that:
When the cosmetics are facial mask, it is made by the steps to obtain:
(1) agitated kettle is added in the raw material in A phases, heating stirring makes each component be sufficiently uniformly dissolved, is cooled to 80~85 DEG C
60℃;
(2) agitated kettle is added in the raw material in B phases when 60 DEG C, is completely dissolved postcooling to 45 DEG C;
(3) raw material in C phases is sequentially added into agitated kettle at 45 DEG C, stirred evenly;
(4) the raw material mixing in D phases is then added, obtains facial mask;
When the cosmetics are Essence, it is made by the steps to obtain:
(1) agitated kettle is added in the raw material in A ' phases, heating stirring makes each component be sufficiently uniformly dissolved to 80~85 DEG C, cooling
To 45 DEG C;
(2) agitated kettle is added in the raw material in B ' phases when 45 DEG C, be completely dissolved;
(3) agitated kettle is added at 45 DEG C in the raw material in C ' phases, stirs evenly, obtains Essence.
9. the preparation method of the cosmetics based on phaffiafhodozyma raw material described in claim 1, it is characterised in that including walking as follows
Suddenly:
(1) it will be mixed with grease from the astaxanthin of phaffiafhodozyma in full of inert gas and dark surrounds, centrifuge, take
Liquid obtains astaxanthin-lipid complex;
(2) by astaxanthin-lipid complex, the yeast extract from phaffiafhodozyma and the Portugal from phaffiafhodozyma
Glycan is added in cosmetics, mixing, obtains the cosmetics based on phaffiafhodozyma raw material.
10. the preparation method of the cosmetics according to claim 9 based on phaffiafhodozyma raw material, it is characterised in that:
Inert gas described in step (1) is nitrogen;
Grease described in step (1) is caprylic/capric triglyceride, isononyl isononanoate, isopropyl myristate, olive
One kind in oil, saualane, Sweet Almond Oil and glycerine or at least two;
The temperature environment of mixing described in step (2) is 40~50 DEG C.
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CN106074200A (en) * | 2016-08-02 | 2016-11-09 | 深圳大学 | A kind of biodiesel extracts natural astaxanthin and the formula of facial film thereof and preparation method |
CN106511134B (en) * | 2016-12-13 | 2019-09-17 | 泉州师范学院 | A kind of astaxanthin facial mask nutrient solution and preparation method thereof |
CN106974844A (en) * | 2017-03-15 | 2017-07-25 | 广州有传生物科技有限公司 | Whitening spot-eliminating composition and its preparation method and application |
CN108324609A (en) * | 2018-03-13 | 2018-07-27 | 云南爱尔康生物技术有限公司 | A kind of moisture retention water of astaxanthin-containing and preparation method thereof |
CN108517345B (en) * | 2018-04-20 | 2021-07-27 | 杭州优玛达生物科技有限公司 | Thermophilic thermus strain and microzyme combined fermentation method |
CN109966189B (en) * | 2019-04-26 | 2021-12-10 | 北京科技大学 | Antioxidant moisturizing microorganism living cell preparation and skin care product containing same |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101897431A (en) * | 2010-06-21 | 2010-12-01 | 中国农业大学 | Method for simultaneously preparing yeast extract and beta-1,3-glucan |
CN103815223A (en) * | 2014-01-23 | 2014-05-28 | 山东百龙创园生物科技有限公司 | Nutrition health-care composition containing astaxanthin |
CN103865798A (en) * | 2014-03-18 | 2014-06-18 | 厦门汇盛生物有限公司 | Method for wall breaking of phaffia rhodozyma through enzymolysis and application thereof |
CN104274428A (en) * | 2013-07-09 | 2015-01-14 | 浙江新维普添加剂有限公司 | Preparation method for oil-dispersible carotenoid preparation |
CN104622713A (en) * | 2015-01-26 | 2015-05-20 | 杭州易文赛生物技术有限公司 | Cosmetic composition and preparation method thereof |
CN105053519A (en) * | 2015-08-06 | 2015-11-18 | 厦门汇盛生物有限公司 | Method for increasing phaffia rhodozyma astaxanthin |
-
2015
- 2015-12-07 CN CN201510896328.0A patent/CN105434319B/en not_active Expired - Fee Related
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101897431A (en) * | 2010-06-21 | 2010-12-01 | 中国农业大学 | Method for simultaneously preparing yeast extract and beta-1,3-glucan |
CN104274428A (en) * | 2013-07-09 | 2015-01-14 | 浙江新维普添加剂有限公司 | Preparation method for oil-dispersible carotenoid preparation |
CN103815223A (en) * | 2014-01-23 | 2014-05-28 | 山东百龙创园生物科技有限公司 | Nutrition health-care composition containing astaxanthin |
CN103865798A (en) * | 2014-03-18 | 2014-06-18 | 厦门汇盛生物有限公司 | Method for wall breaking of phaffia rhodozyma through enzymolysis and application thereof |
CN104622713A (en) * | 2015-01-26 | 2015-05-20 | 杭州易文赛生物技术有限公司 | Cosmetic composition and preparation method thereof |
CN105053519A (en) * | 2015-08-06 | 2015-11-18 | 厦门汇盛生物有限公司 | Method for increasing phaffia rhodozyma astaxanthin |
Non-Patent Citations (1)
Title |
---|
可溶性酵母葡聚糖的制备及其活性研究;余奕珂等;《中国生化药物杂志》;20101231;第31卷(第4期);第254-257页 * |
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