CN105433300A - Processing method for producing active calcium from oyster shells - Google Patents
Processing method for producing active calcium from oyster shells Download PDFInfo
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- CN105433300A CN105433300A CN201511015427.XA CN201511015427A CN105433300A CN 105433300 A CN105433300 A CN 105433300A CN 201511015427 A CN201511015427 A CN 201511015427A CN 105433300 A CN105433300 A CN 105433300A
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- oyster shell
- processing method
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- calcium activated
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/618—Molluscs, e.g. fresh-water molluscs, oysters, clams, squids, octopus, cuttlefish, snails or slugs
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/06—Aluminium, calcium or magnesium; Compounds thereof, e.g. clay
- A61K33/10—Carbonates; Bicarbonates
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- Pharmacology & Pharmacy (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
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- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
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- Zoology (AREA)
- Marine Sciences & Fisheries (AREA)
- Inorganic Chemistry (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention provides a processing method for producing active calcium from oyster shells. According to the method, the oyster shells are used as raw materials, cleaned and subjected to enzymolysis, enzyme deactivation, filtration, drying and crushing, and the active calcium is obtained. The obtained active calcium can be used as a calcium enhancer for food or a raw material for drugs, resources are fully utilized, and waste is turned into wealth. The reaction condition is mild, the raw material source is wide, and the processing method is low in cost, simple to operate and applicable to industrial production and has very high economic value.
Description
Technical field
The present invention relates to marine product deep process technology field, be specifically related to the processing method that a kind of oyster shell produces calcium activated.
Background technology
Male whetstone delicious flavour, nutritious, have the good reputation of " ocean milk ", in its consumption, just make use of the edible portion of male whetstone, but the oyster shell occupying oyster 90% is often abandoned by as rubbish, bulk deposition is in places such as beach, not only occupy valuable soil and Tidal Flat Resources, cause the wasting of resources, also can rot because of it so that cause environmental pollution, have a strong impact on the development of male whetstone aquaculture.
The material composition of oyster shell mainly can be divided into two large classes, and a class has been the organic matter fractions of adjustment and framework effect, and another kind of is inanimate matter part.Organic matter fractions in oyster shell is mainly by protein, and glycoprotein and polysaccharide form; In inanimate matter part, the content of calcium carbonate accounts for more than 90%, oyster shell is also containing amino acid and iron, zinc, selenium and other trace elements such as glycine, methyl amimoacetic acid, methionine in addition, these amino acid and trace element have important physiological action to human body, therefore, oyster shell has potential using value in health food and medicine.
Summary of the invention
The object of this invention is to provide a kind of oyster shell and produce the processing method of calcium activated, adopt biology enzyme enzymatic isolation method, obtaining can for the bulk drug of the Creta Preparata of food or medicine, and components utilising resource, turns waste into wealth.
In order to achieve the above object, the technical scheme that the present invention takes is:
Oyster shell produces a processing method for calcium activated, comprises the steps:
(1) select the complete oyster shell that color and luster is good, individual greatly, matter is thick, removing is attached to earth and the epidermis color spot on surface, cleans up, drain excessive moisture and smash to pieces to 5 ~ 10mm for subsequent use with tissue mashing machine with distilled water;
(2) mixed by certain solid-to-liquid ratio with distilled water by the oyster shell smashed to pieces, regulate pH, heat temperature raising, then adds alkali protease, water bath with thermostatic control vibration alkali protease enzymolysis;
(3) after enzymolysis terminates, enzymolysis liquid goes out enzyme in a heated condition, filters, repeatedly uses distilled water flushing sediment, be washed till neutrality;
(4) sediment is dried, be cooled to 30 ~ 40 DEG C, then pulverize with micronizer, the ultra micro oyster shell powder after pulverizing is sieved and get final product.
As specializing, the solid-to-liquid ratio in described step (2) is 1:2 ~ 5.
As specializing, described step (2) neutral and alkali protease is that 2000 ~ 5000U/g adds by enzyme-to-substrate ratio.
As specializing, in described step (2), regulate pH to 8.0 ~ 9.5.
As specializing, described step is heated to 50 ~ 70 DEG C in (2).
As specializing, after adding alkali protease in described step (2), be incubated enzymolysis 3 ~ 5h.
As specializing, in described step (3), enzymolysis liquid is heated to 95 ~ 100 DEG C, and go out enzyme 15 ~ 20min.
As specializing, in described step (4), sediment is heated to 105 ~ 110 DEG C and dries 5 ~ 8h.
As specializing, the aperture of sieving in described step (4) is 200 orders.
Compared with prior art, beneficial effect of the present invention:
The present invention take oyster shell as raw material, adopts biology enzyme enzymatic isolation method, obtains for the bulk drug of the Creta Preparata of food or medicine, to make full use of resource, turning waste into wealth.Reaction condition is gentle, and raw material sources are wide, and cost is low, simple to operate, can be applicable to suitability for industrialized production, has very high economic worth.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is further described.
Embodiment 1
(1) select the complete oyster shell that color and luster is good, individual greatly, matter is thick, removing is attached to earth and the epidermis color spot on surface, cleans up, drain excessive moisture and smash to pieces to 5 ~ 10mm for subsequent use with tissue mashing machine with distilled water;
(2) be that 1:2 mix with distilled water by solid-to-liquid ratio by the oyster shell smashed to pieces, regulate pH=8.0, be heated to 50 DEG C, then add enzyme-to-substrate than the alkali protease for 2000U/g, water bath with thermostatic control vibration alkali protease enzymolysis 3h;
(3) after enzymolysis terminates, enzymolysis liquid goes out enzyme 20min under 95 DEG C of conditions, filters, repeatedly uses distilled water flushing sediment, be washed till neutrality;
(4) sediment 105 DEG C is dried 5h, be cooled to 30 DEG C, then pulverize with micronizer, the ultra micro oyster shell powder after pulverizing is sieved by 200 mesh sieve and get final product.
Embodiment 2
(1) select the complete oyster shell that color and luster is good, individual greatly, matter is thick, removing is attached to earth and the epidermis color spot on surface, cleans up, drain excessive moisture and smash to pieces to 5 ~ 10mm for subsequent use with tissue mashing machine with distilled water;
(2) be that 1:2.5 mix with distilled water by solid-to-liquid ratio by the oyster shell smashed to pieces, regulate pH=8.2, be heated to 53 DEG C, then add enzyme-to-substrate than the alkali protease for 2300U/g, water bath with thermostatic control vibration alkali protease enzymolysis 3.5h;
(3) after enzymolysis terminates, enzymolysis liquid goes out enzyme 18min under 96 DEG C of conditions, filters, repeatedly uses distilled water flushing sediment, be washed till neutrality;
(4) sediment 108 DEG C is dried 5.5h, be cooled to 34 DEG C, then pulverize with micronizer, the ultra micro oyster shell powder after pulverizing is sieved by 200 mesh sieve and get final product.
Embodiment 3
(1) select the complete oyster shell that color and luster is good, individual greatly, matter is thick, removing is attached to earth and the epidermis color spot on surface, cleans up, drain excessive moisture and smash to pieces to 5 ~ 10mm for subsequent use with tissue mashing machine with distilled water;
(2) be that 1:3 mix with distilled water by solid-to-liquid ratio by the oyster shell smashed to pieces, regulate pH=8.4, be heated to 55 DEG C, then add enzyme-to-substrate than the alkali protease for 2500U/g, water bath with thermostatic control vibration alkali protease enzymolysis 4h;
(3) after enzymolysis terminates, enzymolysis liquid goes out enzyme 15min under 98 DEG C of conditions, filters, repeatedly uses distilled water flushing sediment, be washed till neutrality;
(4) sediment 110 DEG C is dried 6h, be cooled to 35 DEG C, then pulverize with micronizer, the ultra micro oyster shell powder after pulverizing is sieved by 200 mesh sieve and get final product.
Embodiment 4
(1) select the complete oyster shell that color and luster is good, individual greatly, matter is thick, removing is attached to earth and the epidermis color spot on surface, cleans up, drain excessive moisture and smash to pieces to 5 ~ 10mm for subsequent use with tissue mashing machine with distilled water;
(2) be that 1:4 mix with distilled water by solid-to-liquid ratio by the oyster shell smashed to pieces, regulate pH=8.7, be heated to 60 DEG C, then add enzyme-to-substrate than the alkali protease for 3000U/g, water bath with thermostatic control vibration alkali protease enzymolysis 4.5h;
(3) after enzymolysis terminates, enzymolysis liquid goes out enzyme 15min under 100 DEG C of conditions, filters, repeatedly uses distilled water flushing sediment, be washed till neutrality;
(4) sediment 108 DEG C is dried 7h, be cooled to 37 DEG C, then pulverize with micronizer, the ultra micro oyster shell powder after pulverizing is sieved by 200 mesh sieve and get final product.
Embodiment 5
(1) select the complete oyster shell that color and luster is good, individual greatly, matter is thick, removing is attached to earth and the epidermis color spot on surface, cleans up, drain excessive moisture and smash to pieces to 5 ~ 10mm for subsequent use with tissue mashing machine with distilled water;
(2) be that 1:4 mix with distilled water by solid-to-liquid ratio by the oyster shell smashed to pieces, regulate pH=9.0, be heated to 65 DEG C, then add enzyme-to-substrate than the alkali protease for 4000U/g, water bath with thermostatic control vibration alkali protease enzymolysis 5h;
(3) after enzymolysis terminates, enzymolysis liquid goes out enzyme 18min under 98 DEG C of conditions, filters, repeatedly uses distilled water flushing sediment, be washed till neutrality;
(4) sediment 110 DEG C is dried 7.5h, be cooled to 30 DEG C, then pulverize with micronizer, the ultra micro oyster shell powder after pulverizing is sieved by 200 mesh sieve and get final product.
Embodiment 6
(1) select the complete oyster shell that color and luster is good, individual greatly, matter is thick, removing is attached to earth and the epidermis color spot on surface, cleans up, drain excessive moisture and smash to pieces to 5 ~ 10mm for subsequent use with tissue mashing machine with distilled water;
(2) be that 1:5 mix with distilled water by solid-to-liquid ratio by the oyster shell smashed to pieces, regulate pH=9.5, be heated to 70 DEG C, then add enzyme-to-substrate than the alkali protease for 5000U/g, water bath with thermostatic control vibration alkali protease enzymolysis 4.5h;
(3) after enzymolysis terminates, enzymolysis liquid goes out enzyme 17min under 97 DEG C of conditions, filters, repeatedly uses distilled water flushing sediment, be washed till neutrality;
(4) sediment 110 DEG C is dried 8h, be cooled to 35 DEG C, then pulverize with micronizer, the ultra micro oyster shell powder after pulverizing is sieved by 200 mesh sieve and get final product.
The above, it is only present pre-ferred embodiments, not technical scope of the present invention is imposed any restrictions, thus every above embodiment is done according to technical spirit of the present invention any trickle amendment, equivalent variations and modification, all still belong in the scope of technical solution of the present invention.
Claims (9)
1. oyster shell produces a processing method for calcium activated, it is characterized in that, comprises the steps:
(1) select the complete oyster shell that color and luster is good, individual greatly, matter is thick, removing is attached to earth and the epidermis color spot on surface, cleans up, drain excessive moisture and smash to pieces to 5 ~ 10mm for subsequent use with tissue mashing machine with distilled water;
(2) mixed by certain solid-to-liquid ratio with distilled water by the oyster shell smashed to pieces, regulate pH, heat temperature raising, then adds alkali protease, water bath with thermostatic control vibration alkali protease enzymolysis;
(3) after enzymolysis terminates, enzymolysis liquid goes out enzyme in a heated condition, filters, repeatedly uses distilled water flushing sediment, be washed till neutrality;
(4) sediment is dried, be cooled to 30 ~ 40 DEG C, then pulverize with micronizer, the ultra micro oyster shell powder after pulverizing is sieved and get final product.
2. oyster shell according to claim 1 produces the processing method of calcium activated, and it is characterized in that, the solid-to-liquid ratio in described step (2) is 1:2 ~ 5.
3. oyster shell according to claim 1 produces the processing method of calcium activated, and it is characterized in that, described step (2) neutral and alkali protease is that 2000 ~ 5000U/g adds by enzyme-to-substrate ratio.
4. oyster shell according to claim 1 produces the processing method of calcium activated, it is characterized in that, regulates pH to 8.0 ~ 9.5 in described step (2).
5. oyster shell according to claim 1 produces the processing method of calcium activated, and it is characterized in that, described step is heated to 50 ~ 70 DEG C in (2).
6. oyster shell according to claim 1 produces the processing method of calcium activated, it is characterized in that, is incubated enzymolysis 3 ~ 5h after adding alkali protease in described step (2).
7. oyster shell according to claim 1 produces the processing method of calcium activated, it is characterized in that, in described step (3), enzymolysis liquid is heated to 95 ~ 100 DEG C, and go out enzyme 15 ~ 20min.
8. oyster shell according to claim 1 produces the processing method of calcium activated, it is characterized in that, in described step (4), sediment is heated to 105 ~ 110 DEG C and dries 5 ~ 8h.
9. oyster shell according to claim 1 produces the processing method of calcium activated, and it is characterized in that, the aperture of sieving in described step (4) is 200 orders.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201511015427.XA CN105433300A (en) | 2015-12-29 | 2015-12-29 | Processing method for producing active calcium from oyster shells |
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| CN201511015427.XA CN105433300A (en) | 2015-12-29 | 2015-12-29 | Processing method for producing active calcium from oyster shells |
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| CN105433300A true CN105433300A (en) | 2016-03-30 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106072661A (en) * | 2016-06-21 | 2016-11-09 | 陆兴弟 | A kind of shellfish solution calcium ion extraction process |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101731666A (en) * | 2010-02-10 | 2010-06-16 | 鞍山嘉鲜农业发展有限公司 | Fishbone bioactive polypeptide calcium powder and preparation method |
| CN102442871A (en) * | 2010-10-12 | 2012-05-09 | 青岛大学 | Preparation method of composite calcium amino acid chelate by hems of shells and scallops as raw materials |
| CN102703554A (en) * | 2012-06-07 | 2012-10-03 | 广西还珠海洋生物科技有限公司 | Method for preparing oyster polypeptide from oyster shell |
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- 2015-12-29 CN CN201511015427.XA patent/CN105433300A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101731666A (en) * | 2010-02-10 | 2010-06-16 | 鞍山嘉鲜农业发展有限公司 | Fishbone bioactive polypeptide calcium powder and preparation method |
| CN102442871A (en) * | 2010-10-12 | 2012-05-09 | 青岛大学 | Preparation method of composite calcium amino acid chelate by hems of shells and scallops as raw materials |
| CN102703554A (en) * | 2012-06-07 | 2012-10-03 | 广西还珠海洋生物科技有限公司 | Method for preparing oyster polypeptide from oyster shell |
Non-Patent Citations (2)
| Title |
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| 潘道东: "《功能性食品添加剂》", 31 December 2006, 中国轻工业出版社 * |
| 邱澄宇: "《水产品加工新技术与营销》", 30 September 2011, 《金盾出版社》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106072661A (en) * | 2016-06-21 | 2016-11-09 | 陆兴弟 | A kind of shellfish solution calcium ion extraction process |
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Application publication date: 20160330 |