CN1054263C - Method for producing lilac variotin fungal preparation from waste liquor of sugar refinery - Google Patents
Method for producing lilac variotin fungal preparation from waste liquor of sugar refinery Download PDFInfo
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- CN1054263C CN1054263C CN95118469A CN95118469A CN1054263C CN 1054263 C CN1054263 C CN 1054263C CN 95118469 A CN95118469 A CN 95118469A CN 95118469 A CN95118469 A CN 95118469A CN 1054263 C CN1054263 C CN 1054263C
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Abstract
The present invention relates to a method for producing an insecticidal agent with sugar refinery waste. In the method, a purified lilac quasi-penicillium strain is inoculated on the oblique plane of a fungi culture medium, and the inoculated strain is inoculated in a culture fluid compounded with molasses diluted to one-eighth to one-sixteenth; after oscillatory culture for several days, the inoculated strain is further adsorbed onto a solid culture medium composed of molasses and bagasse and is cultivated at 25 to 30 DEG C for 5 to 15 days to form a lilac quasi-penicillium preparation used as the insecticidal agent which can be used for the nematode control of a plurality of agricultural crops and can effectively reduce the nematode population and increase the crop yield. Meanwhile, due to that the lilac quasi-penicillium strain is produced with the sugar refinery waste, the sugar refinery waste is recycled so that the environment pollution is reduced.
Description
The present invention relates to a kind of method of utilizing microbial fungi to produce insecticide.
Plant nematode makes crop production reduction, and by the most conservative estimation, national proportion of crop planting industry is lost 23,300,000,000 yuan every year approximately.At present to the control of nematodosis mainly based on chemical nematicide.Though certain effect is arranged, easily cause environmental pollution, and pollute agricultural product, cause the incident of poisoning behind the food agricultural produce to happen occasionally.Given this, people begin to look for new method in addition from ecological view, adopt the control of measure of biotic control substituted chemistry to become inexorable trend.The natural enemy kind of nematode is a lot, mainly contains fungi, bacterium, virus etc.A kind of inner parasitic epiphyte that destroys nematode is arranged in fungi---Paecilomyces lilacinus (Paecilomyces lilacinus), this bacterium can infect the ovum of root-knot nematode (Meloidogyne SPP.) and Cyst nematode important Plant nematodes such as (Globodera SPP. and Heterodera SPP.) and absorb material in its ovum, the incubation rate of ovum is reduced, the quantity of root insect gall and pieces of an egg reduces, and therefore uses its next control for harmful nematode and has great potential.
Purpose of the present invention aims to provide a kind of waste material with sugar refinery---and molasses and bagasse manufacturing can prevent and treat the method for the Paecilomyces lilacinus microbial inoculum of economic crops parasitic nematode, this method both can provide the nematicide medicine of high effect nontoxic, can solve the waste disposal problem in sugar refinery again.
Technical scheme of the present invention is as follows:
Step 1: one-level is cultivated, and common fungi culture medium is distributed into the inclined-plane, and inoculation Paecilomyces lilacinus bacterial classification was cultivated 5～15 days under 20～30 ℃ of temperature, and it is good cultivating 7 days with 28 ℃.
Step 2: secondary is cultivated, molasses are diluted to 1/8～1/16, or blackstrap mixes 1/10～1/20 molasses or other carbon sources, PH transfers to 5.5～7, with 6～6.5 the bests, after the mixing, inoculation one-level culture behind conventional autoclaving, shaken cultivation is 5～10 days under 25～30 ℃ of temperature, and optimum condition is 28 ℃ of following shaken cultivation 7 days.
Step 3: three grades of cultivations, to be diluted to 1/8～1/16 molasses or mix thoroughly by 1/10～1/20 blackstrap and the bagasse that adds molasses or other carbon sources, become to squeeze the powder ball that does not drip, behind moist heat sterilization, the secondary culture fluid is adsorbed up, under 25～30 ℃ of temperature, cultivated again 5～15 days, generally cultivate 10 days the bests with 28 ℃, air-dry back becomes the cake bulk, lavender is scattered easily, all can with powdery or block preservation of cake.
With the Paecilomyces lilacinus microbial inoculum that this method is produced, every gram sample contains 2.6 * 10
6～3.4 * 10
6Individual spore.Dosage by 4g/Kg soil was admixed the microbial inoculum pulverizing in the soil before crop is transplanted, and can effectively reduce insect density (pieces of an egg and female worm all reduce more than 90% than control group for potted plant tomato test, the insect gall of experimental group).The Paecilomyces lilacinus microbial inoculum is as a kind of novel biological nematocide, can be used for the nematoda control of many crops such as soybean, beet, potato, wheat class, corn, cotton, peanut, banana, citrus, pineapple, tea shoot, sugarcane, sweet potato, tobacco, red bayberry, Momordica grosvenori, kiwi fruit, peach, tomato, capsicum, eggplant, persimmon, carrot, grape, strawberry, fig, cucumber, pumpkin, muskmelon, watermelon, balsam pear, Kidney bean, not only improve output, and avoid using chemical nematicide.This microbial inoculum of liquid and waste slag produced manufacturing with sugar refinery can make waste resource recovery, has both reduced industrial cost, reduces environmental pollution again.
The invention will be further described below in conjunction with embodiment.
Embodiment 1: the Paecilomyces lilacinus bacterial classification inoculation of purifying is arrived on potato-glucose agar medium (PDA) inclined-plane, cultivated 7 days down at 28 ℃; Add water 87.5ml with the molasses 12.5ml in sugar refinery and be mixed with culture fluid, PH transfers to 6.3, mixes after the above-mentioned one-level culture of inoculation behind the conventional autoclaving, and shaken cultivation is 7 days under 28 ℃ of conditions; Mix thoroughly with bagasse after again the molasses of 12.5ml being mixed 87.5ml water, the addition of bagasse is adsorbed up the secondary culture fluid behind moist heat sterilization to squeeze the degree of being that do not drip, and cultivates under 28 ℃ 10 days again, and air-dry back becomes the cake bulk.Take by weighing the 1g microbial inoculum, place and stir diffusingly in the high-speed tissue mashing machine, wash out constant volume 500ml with clear water then, draw a small amount of microscopy counting on blood counting chamber that drips, every as calculated g microbial inoculum contains 3.5 * 10
6Individual spore.
Embodiment 2: the one-level of Paecilomyces lilacinus bacterium, secondary are cultivated with embodiment 1, get the 8.3ml molasses and add 91.7ml water in three grades of cultivations, adding bagasse is mixed thoroughly to squeezing and is not dripped, and behind moist heat sterilization the secondary culture fluid is adsorbed up, cultivated under 28 ℃ 10 days, air-dry back becomes the cake bulk again.Every after measured gram microbial inoculum contains 3.0 * 10
6Individual spore.
Embodiment 3: the proportioning of three grades of cultures as different from Example 1, and get the 6.3ml molasses and add 93.7ml water, add bagasse and mix thoroughly, all the other methods and condition are with embodiment 1, and every after measured gram microbial inoculum contains 2.3 * 10
6Individual spore.
Embodiment 4: inoculation Paecilomyces lilacinus bacterium bacterial classification on potato-glucose agar medium (PDA) inclined-plane, cultivated 15 days down at 20 ℃; Add 91.7ml water with the 8.3ml molasses and make liquid medium, PH transfers to 5.5, the culture on inoculation PDA behind the autoclaving, and shaken cultivation is 10 days under 25 ℃ of temperature; Add 87.5ml water again with bagasse with the 12.5ml molasses, the above-mentioned culture of inoculation was cultivated 15 days under 25 ℃ of temperature again behind the moist heat sterilization, and air-dry back becomes the cake bulk.
Embodiment 5: inoculation Paecilomyces lilacinus bacterium bacterial classification on the PDA medium slant, cultivated 5 days down at 30 ℃, be inoculated in the culture fluid that is made into by 6.3ml molasses and 93.7ml water, PH transfers to 7, in 30 ℃ of following shaken cultivation 5 days, get the 12.5ml molasses, 87.5ml water is made solid culture with bagasse, inoculates above-mentioned culture, cultivated 5 days down in 30 ℃.
Embodiment 6: Paecilomyces lilacinus microbial inoculum one-level is cultivated with embodiment 1, in secondary and the three grades of cultivations molasses is changed into the 90ml blackstrap adding the 10ml molasses, and all the other conditions are constant.
Embodiment 7: Paecilomyces lilacinus microbial inoculum one-level is cultivated with embodiment 1, in secondary and three grades of cultivations molasses is made into to add the 5ml molasses with the 95ml blackstrap, and all the other conditions are constant.
Claims (2)
1. method of producing the Paecilomyces lilacinus microbial inoculum with waste material from sugar refinery is characterized in that:
Step 1: one-level is cultivated, and dresses up the inclined-plane with common fungi culture medium, and inoculation Paecilomyces lilacinus bacterial classification was cultivated 5～15 days under 20～30 ℃ of temperature;
Step 2: secondary is cultivated, and molasses are diluted to 1/8～1/16, or blackstrap mixes 1/10～1/20 molasses, and PH transfers to 5.5～7, after the mixing, and inoculation one-level culture behind conventional autoclaving, shaken cultivation is 5～10 days under 25～30 ℃ of temperature;
Step 3: three grades of cultivations, with being diluted to 1/8～1/16 molasses or adding molasses or blackstrap and bagasse by 1/10～1/20 and mix thoroughly,, behind moist heat sterilization, the secondary culture fluid is adsorbed up to squeeze the degree of being that do not drip, cultivated 5～15 days under 25～30 ℃ of temperature, air-dry back becomes the cake bulk again.
2. method of producing the Paecilomyces lilacinus microbial inoculum with waste material from sugar refinery as claimed in claim 1 is characterized in that the one-level cultivation was with 28 ℃ of cultivations 7 days; The culture fluid that secondary is cultivated is diluted to 1/8 with molasses, and PH transfers to 6～6.5, and condition of culture is 28 ℃, shaken cultivation 7 days; Three grades of cultivations are diluted to 1/8,28 ℃ with molasses and cultivated 7 days down.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN95118469A CN1054263C (en) | 1995-10-27 | 1995-10-27 | Method for producing lilac variotin fungal preparation from waste liquor of sugar refinery |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN95118469A CN1054263C (en) | 1995-10-27 | 1995-10-27 | Method for producing lilac variotin fungal preparation from waste liquor of sugar refinery |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1149394A CN1149394A (en) | 1997-05-14 |
| CN1054263C true CN1054263C (en) | 2000-07-12 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN95118469A Expired - Fee Related CN1054263C (en) | 1995-10-27 | 1995-10-27 | Method for producing lilac variotin fungal preparation from waste liquor of sugar refinery |
Country Status (1)
| Country | Link |
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| CN (1) | CN1054263C (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101845398B (en) * | 2010-06-17 | 2012-07-11 | 中国热带农业科学院环境与植物保护研究所 | Method for culturing paecilomyces lilacinus |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1991002051A1 (en) * | 1989-08-03 | 1991-02-21 | The Australian Technological Innovation Corporation | Myconematicide |
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1995
- 1995-10-27 CN CN95118469A patent/CN1054263C/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1991002051A1 (en) * | 1989-08-03 | 1991-02-21 | The Australian Technological Innovation Corporation | Myconematicide |
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| CN1149394A (en) | 1997-05-14 |
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