CN105424671A - Preparation method of algae suspension stable sample for fluorescent measurement - Google Patents
Preparation method of algae suspension stable sample for fluorescent measurement Download PDFInfo
- Publication number
- CN105424671A CN105424671A CN201610014072.0A CN201610014072A CN105424671A CN 105424671 A CN105424671 A CN 105424671A CN 201610014072 A CN201610014072 A CN 201610014072A CN 105424671 A CN105424671 A CN 105424671A
- Authority
- CN
- China
- Prior art keywords
- algae liquid
- sample
- algae
- frond
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Engineering & Computer Science (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention discloses a preparation method of an algae suspension stable sample for fluorescent measurement, and belongs to the technical field of algae liquid measurement. The method includes the steps of preparing an algae liquid sample through to-be-sampled algae liquid, measuring chlorophyll in the algae liquid sample through a fluorescent method, conducting centrifuging treatment on the to-be-sampled algae liquid, and preparing an algae liquid sample through algae obtained through centrifuging treatment and a suspension stabilizer, wherein the suspension stabilizer is prepared from agar, xanthan gum and carrageenan. By adding the suspension stabilizer to the detected algae liquid in the fluorescent detecting process, all parameters are measured through florescence, the fluorescence value is small in attenuation rate and fluctuation, it is shown that the algae is high in suspension stability and stable in system, and the algae liquid measurement is more accurate.
Description
Technical field
The invention belongs to the technical field of water sample measuring chlorophyll content, relate to a kind of method of fluorescence spectrometry water sample chlorophyll content, be the preparation method of the suspending stabilized sample of a kind of frond for fluorescence measurement more specifically, this method adds suspension, the stability of algae liquid, obtains finely dispersed algae liquid sample.
Background technology
Water body Determination of Chlorophyll a content is routine monitoring project in water quality environmental monitoring, is one of body eutrophication index, reacts amount of algae and water quality condition in water body to a certain extent.Chlorophyll-a Content Accurate Measurement is particularly important.Fluorescence spectrophotometry is due to highly sensitive, simple to operate, can continuously in real time, monitoring, and achieving the real-time in-situ analysis of water body Determination of Chlorophyll, is the method the most conventional that experimental determination chlorophyll a realizes the quantitative measurement to phytoplankton.
There are the problems such as frustule dispersion is uneven, free settling, suspension stability difference in actual sample chlorophyll measuring process, cause fluoroscopic examination Chlorophyll-a Content accuracy of measurement not high, cannot use fluorescent value accurate response algae liquid concentration, this is a problem of water sample chlorophyll measuring always.When finding in experimentation that prior art fluorescence detection directly measures algae liquid sample and actual water sample, it is inaccurate that frustule sedimentation obviously, measured value changes greatly the data obtained in time, correctly cannot judge water quality environment, therefore correct measurement water quality environment is most important.Therefore, reduce the settling velocity of the frustule disperseed, the suspension, the stability that increase algae liquid obtain the emphasis that finely dispersed algae liquid sample becomes research.
Summary of the invention
When the present invention is for solving prior art fluorescence spectrometry water body sample chlorophyll content, because frustule is uneven in water body, free settling, suspension system unstable, the problem that measurement result is inaccurate, error is large, provide the preparation method of the suspending stabilized sample of a kind of frond for fluorescence measurement, efficiently solve the problems referred to above.
The present invention is the technical scheme realizing the employing of its object:
The preparation method of the suspending stabilized sample of a kind of frond for fluorescence measurement, first adopt and prepare algae liquid sample for examination algae liquid, then by fluorescence method, chlorophyll is measured, first will carry out centrifugal treating for examination algae liquid, then centrifugal treating is obtained frond and suspension stabilizer is configured to algae liquid sample, wherein said suspension stabilizer is made up of agar, xanthans and carragheen.
Get and be placed in hydro-extractor for examination algae liquid, with the centrifugal 15-20min of the rotating speed of 3500-4500r/min, then outwell supernatant, obtain frond.
Agar in described suspension stabilizer: xanthans: the content of carragheen is than being (2-10): (10-30): (30-50), and in sample algae liquid, the concentration of agar is 0.06%.
Agar in described suspension stabilizer: xanthans: the content ratio of carragheen is 3:7.5:17.5, and in sample algae liquid, the concentration of agar is 0.06%.
Described is chlorella pyrenoidosa algae liquid for examination algae liquid, as laboratory cultures algae liquid sample, before centrifugal treating, is first seeded in the conical flask filling sterilizing BG-11 nutrient solution by chlorella pyrenoidosa in chlorella pyrenoidosa algae liquid and cultivates.
Described takes from man-made lake water sample for examination algae liquid, as actual water sample, when taking from man-made lake water sample for during for examination algae liquid, will immediately for the preparation of algae liquid sample after sampling, or as placed, add 1mL1% magnesium carbonate suspending liquid to prevent chlorophyll acidifying in every premium on currency sample, and keep in Dark Place in 4 DEG C of refrigerators.
The invention has the beneficial effects as follows: the present invention is by fluorescence detection, the creationary algae liquid by detection adds suspension stabilizer, by each parameter of fluorometric assay, the attenuation rate of fluorescent value is little, it is little to fluctuate, show that the suspension stability of algae liquid is high, algae liquid system is stablized, more accurate to the mensuration of algae liquid.
Through long-term creative research, inventor finds between different suspension stabilizers, even if the viscosity of solution is identical or close, liquid suspension stability is not identical yet, even difference is with large, but it is relevant remarkable between suspension to stability, the mensuration of suspension stability on algae liquid of algae liquid has great impact, agar is selected by long-term creative research the present invention, xanthans, carragheen is suspension stabilizer, also be auxiliary rheological agents, be mainly used in the viscosity improving and increase liquid, thus change the physical behavior of solution, keep the stability of fluid, there is emulsification, homogeneous, stablize and present the effect of suspended state.And in prior art, suspension stability is not considered to the mensuration of algae liquid, it is substantially all being considered the problem of impurity and absorbance and is being undertaken by the precision of measuring apparatus, without any the problem of algae liquid suspension.
Embodiment
When the present invention is for solving prior art fluorescence spectrometry water body chlorophyll, because frustule is uneven in water body, free settling, suspension system unstable, the problem that measurement result is inaccurate, error is large, provide the preparation method of the suspending stabilized sample of a kind of frond for fluorescence measurement, efficiently solve the problems referred to above, below in conjunction with specific embodiment, the invention will be further described.
One, experimental apparatus and reagent
Instrument: illumination box (E-30B0 U.S. Percival); Visible spectrophotometer (722 type Shanghai Spectrum Apparatus Co., Ltd.); Hydro-extractor (Z323 type Hermle); Ultraviolet spectrophotometer (UV-2600 Shanghai Techcomp Instrument Ltd.); Fluorospectrophotometer (Shimadzu Corporation of RF-5301 Japan).
Reagent: hydrochloric acid 1mol.L
-1; Nutrient culture media is sterilizing BG-11 nutrient solution; 10g.L
-1magnesium carbonate suspending liquid.
Suspending agent: agar, food-grade; Xanthans, food-grade; Carragheen, food-grade.
Fluorospectrophotometer fluorometric assay setting parameter: excitation wavelength is 436.0nm; Emission wavelength is 672.0nm; Excite and launch slit width and be 5.0nm; Sensitivity: high; Response time: Auto.
Embodiment 1. is for examination algae with chlorella pyrenoidosa
The chlorella pyrenoidosa chosen (buy from Chinese Academy of Sciences's wildlife matter storehouse---algae kind storehouse) be algae kind, algae kind is seeded in the conical flask filling sterilizing BG-11 nutrient solution, then conical flask is placed in E-30B0 illumination box to cultivate, condition of culture is: cultivation temperature 25 DEG C, illumination condition 2000lx, set of time be 12h daytime/12h night, damp condition 75%RH, algae kind quiescent culture, shaking conical flask every day and change conical flask position 2 times simultaneously, taking out as supplying examination algae liquid after ten days simultaneously;
Get 25mL and be placed in hydro-extractor for examination algae liquid, with the centrifugal 15min of the rotating speed of 4000r/min, then supernatant is outwelled, obtain frond, then the frond obtained and suspension stabilizer, water are configured to algae liquid sample, having configured 100mL algae liquid sample is example, and wherein the concentration of agar is 0.06%, the concentration of xanthans is 0.2%, and the concentration of carragheen is 0.4%; Then by fluorescence method, algae liquid is measured.
Two, the foundation of chlorella pyrenoidosa algae liquid typical curve
According to molecular absorption spectrum and the fluorescence spectrum transition principle of material, be 436nm in excitation wavelength, chlorophyll a sends the fluorescence of 672nm, and when exciting light is stablized, fluorescence intensity only has correlationship with the concentration of algae liquid, can this excite with emission wavelength under measure the concentration of algae liquid.But have during measurement less of the too low fluorescent value of algae liquid concentration that error is not allowed to ignore, algae liquid excessive concentration time fluorescent value be that slow ascendant trend even declines and causes numerical value inaccurate because there occurs concentration quenching.Therefore optimum D (663nm) scope of its correspondence and the fluorescence intensity level scope of correspondence to be determined according to the typical curve of chlorella pyrenoidosa.
The chlorella pyrenoidosa algae liquid of taking the logarithm growth period, dilutes with the BG-11 nutrient culture media of sterilizing, and extension rate is 1-20, measures the absorbance of algae sample series with 722 type visible spectrophotometers in the chlorophyll a characteristic absorption wavelength place of 663nm; Under the fluorometric assay parameter set, measure the fluorescence intensity level of algae sample series with RF5301 fluorospectrophotometer, draw the situation of change of fluorescence intensity level with its concentration (the absorbance reaction by chlorophyll a characteristic wave strong point) of chlorella pyrenoidosa.
Utilize 722 type spectrophotometers and the Shimadzu RF5301 fluorescent spectrophotometer assay variable concentrations live body absorbance of algae sample at optimal absorption wavelength place and the relation of fluorescence intensity, when having shown that the D (663nm) of chlorella pyrenoidosa sample is in 0.3 ~ 0.8 scope and fluorescence intensity level be good positive correlation, corresponding fluorescence intensity scope is 40 ~ 77.
Three, the characteristic absorption of suspension stabilizer is determined
Use UV-2600 type ultraviolet-visible pectrophotometer under room temperature, scan 0.1% agar solution, 0.3% xanthan gum solution and 0.5% carrageenan solutions, the absorbance in 220 ~ 900nm wavelength coverage, determines without characteristic absorption.Therefore the interpolation of agar, xanthans and carragheen on frond spectrodensitometry result without impact.
Four, time of repose impact that fluorescent value is measured
The object of this experiment is: see whether frustule precipitates within a certain period of time, whether suspension effect is good: get 25mL chlorella pyrenoidosa algae liquid, 100mL is settled to the BG-11 nutrient culture media of sterilizing, the BG-11 nutrient culture media of sterilizing is as blank reference, use Shimadzu RF5301 fluorospectrophotometer under the fluorometric assay parameter set, record initial fluorescent intensity value F
0=89.383, the fluorescence intensity of an algae stoste is measured every 1min.Set up the relation curve between interval time of measurement (T) and fluorescent value (F), set up the relation table between interval time of measurement and fluorescent value, in table 1
Relation between table 1 interval time of measurement and fluorescent value
As shown in Table 1, fluorescent value changes greatly with interval time of measurement, and during T=1min, the attenuation rate of fluorescent value reaches △ F/F
0=8.75%, there is comparatively big error in measured value.This is because frustule sedimentation in standing 1min is very fast, after 5min, algae stoste system tends towards stability substantially, must shake up, requires fast, to reduce the error because frustule causes because of sedimentation when measuring before therefore requiring working sample.
Five, the algae liquid sample stability research of suspension stabilizer
Choose 3 kinds of suspension stabilizers, first test with single suspension stabilizer, agar powder (0.02-0.10g/100mL), xanthans (0.10-0.30g/100mL) and carragheen (0.30-0.50g/100mL).According to the using dosage standard of the frustule content in algae liquid sample and relevant suspension stabilizer, choose variable concentrations gradient and carry out parallel laboratory test.After tentatively determining the stabilizing agent of applicable chlorella pyrenoidosa, then carry out refinement experiment, determine each stabilizing agent addition and suspension effect, as shown in table 2-table 4.
5.1 agar are to the research of algae liquid sample stability
Get the chlorella pyrenoidosa liquid that 5 parts of upgrowth situations are identical, every part of 25mL, centrifugal 15min under 4000r/min rotating speed, then take out the agar solution A that frond uses variable concentrations respectively
1 #, A
2 #, A
3 #, A
4 #, A
5 #be settled to 100mL, as algae liquid sample.Use Shimadzu RF5301 fluorospectrophotometer under the fluorometric assay parameter determined, measure the fluorescence intensity level of a sample every 1min, and ask its attenuation rate, and record the time that attenuation rate reaches 5%.
Table 2 agar is on the impact of algae liquid sample suspension stability
As shown in Table 2, make suspension stabilizer with agar powder, make its addition within the scope of 0.08-0.10g/100mL, chlorella pyrenoidosa can be made to suspend evenly, the good stability of algae suspension system.
5.2 xanthans are to the research of algae liquid sample stability
Get the chlorella pyrenoidosa liquid that 5 parts of upgrowth situations are identical, every part of 25mL, at 4000r.min
-1centrifugal 15min under rotating speed, then takes out the xanthan gum solution C that frond uses variable concentrations respectively
1 #, C
2 #, C
3 #, C
4 #, C
5 #be settled to 100mL, use Shimadzu RF5301 fluorospectrophotometer under the fluorometric assay parameter determined, measure the fluorescence intensity level of a sample every 1min, and ask its attenuation rate, and record the time that attenuation rate reaches 5%.
Table 3 xanthans is on the impact of algae liquid sample suspension stability
As shown in Table 3, make suspension stabilizer with xanthans, make its addition be 0.25-0.30g/100mL, chlorella pyrenoidosa can be made to suspend evenly, algae suspension system good stability.
5.3 carragheens are to the research of algae liquid sample stability
Get the chlorella pyrenoidosa liquid that 5 parts of upgrowth situations are identical, every part of 25mL, at 4000r.min
-1centrifugal 15min under rotating speed, then takes out the carrageenan solutions D that frond uses variable concentrations respectively
1 #, D
2 #, D
3 #, D
4 #, D
5 #be settled to 100mL, use Shimadzu RF5301 fluorospectrophotometer under the fluorometric assay parameter determined, measure the fluorescence intensity level of a sample every 1min, and ask its attenuation rate, and record the time that attenuation rate reaches 5%.
Table 4 carragheen is on the impact of algae liquid sample suspension stability
As shown in Table 4, make suspension stabilizer with carragheen, make its addition be 0.45-0.50g/100mL, chlorella pyrenoidosa can be made to suspend evenly, and algae suspension system good stability, transparency is good.
The algae liquid sample stability research of 5.4 composite suspension stabilizing agents
On the basis of experiment of single factor, synthetic study is carried out to agar, xanthans, carragheen three kinds of suspension stabilizers.Combine its mobility and suspending stabilized ability with agar and xanthans strong, but be long placed in and there will be supernatant.Configuration composite suspension agent, namely agar, xanthans, carragheen carry out synthetic study, optimize algae liquid sample stabilising system.
Table 5 composite suspension agent orthogonal test factor and level
Table 6 composite suspension agent orthogonal experiments
According to the result of table 5, table 6 orthogonal test, by range analysis R
1> R
2> R
3known, in composite suspension agent, the factor that the suspending stabilized influential effect of algae liquid is descending is followed successively by trial stretch: agar > xanthans > carragheen, formula A
3b
2c
2for optimum combination, i.e. agar 0.06%, xanthans 0.15%, carragheen 0.35%, now the rate of change of fluorescent value is-1.16%.
Embodiment 2. actual water sample is for supplying examination algae
Gather the artificial lake water 4L of Hebei University of Science and Technology as actual water sample, be equally divided into 4 parts, obtain water sample 1, water sample 2, water sample 3, water sample 4, being placed in hydro-extractor carries out centrifugal, outwells supernatant and obtains frond, frond and suspension stabilizer, water is configured, constant volume is to 100mL, abundant stirring, makes frustule be evenly distributed in suspension liquid, obtains algae liquid sample.With the fluorescence intensity level of RF5301 fluorescent spectrophotometer assay algae liquid sample, by algae content in its fluorescent value intensity reaction water body, and according to the rate of change of fluorescence intensity level and the relative standard deviation of measured value, the suspension stability of reflection algae liquid sample solution.
The mensuration of actual water sample
The water sample 1-4 adding suspension stabilizer is measured by fluorescence spectrophotometry with the actual water sample 0 of not adding suspension stabilizer, and studies the stability of suspension system, as shown in table 7 below.
Relation between table 7 actual water sample interval time of measurement and fluorescent value
Can obtain from table 7, by the stability of the fluctuation situation reaction suspension system of fluorescent value, obtain in the experiment of 1h time stability, its fluorescent value of the actual water sample fluctuation not adding suspension stabilizer is larger, and the rate of change adding its fluorescent value of actual water sample of suspension stabilizer in ± 3.5%, precision is higher within 1.5%, the interpolation of suspension stabilizer can make frustule suspend evenly.
Claims (6)
1. the preparation method of the suspending stabilized sample of the frond for fluorescence measurement, first adopt and prepare algae liquid sample for examination algae liquid, then by fluorescence method, chlorophyll is measured, it is characterized in that: first will carry out centrifugal treating for examination algae liquid, then centrifugal treating is obtained frond and suspension stabilizer is configured to algae liquid sample, wherein said suspension stabilizer is made up of agar, xanthans and carragheen.
2. the preparation method of the suspending stabilized sample of a kind of frond for fluorescence measurement according to claim 1, it is characterized in that: get and be placed in hydro-extractor for examination algae liquid, with the centrifugal 15-20min of the rotating speed of 3500-4500r/min, then outwell supernatant, obtain frond.
3. the preparation method of the suspending stabilized sample of a kind of frond for fluorescence measurement according to claim 1, it is characterized in that: agar in described suspension stabilizer: xanthans: the content of carragheen is than being (2-10): (10-30): (30-50), and in sample algae liquid, the concentration of agar is 0.06%.
4. the preparation method of the suspending stabilized sample of a kind of frond for fluorescence measurement according to claim 1, it is characterized in that: agar in described suspension stabilizer: xanthans: the content ratio of carragheen is 3:7.5:17.5, and in sample algae liquid, the concentration of agar is 0.06%.
5. the preparation method of the suspending stabilized sample of a kind of frond for fluorescence measurement according to claim 1, it is characterized in that: described is chlorella pyrenoidosa algae liquid for examination algae liquid, as laboratory cultures algae liquid sample, before centrifugal treating, first chlorella pyrenoidosa in chlorella pyrenoidosa algae liquid is seeded in the conical flask filling sterilizing BG-11 nutrient solution and cultivates.
6. the preparation method of the suspending stabilized sample of a kind of frond for fluorescence measurement according to claim 1, it is characterized in that: described takes from man-made lake water sample for examination algae liquid, as actual water sample, when taking from man-made lake water sample for during for examination algae liquid, will immediately for the preparation of algae liquid sample after sampling, or as placed, add 1mL1% magnesium carbonate suspending liquid in every premium on currency sample, and keep in Dark Place in 4 DEG C of refrigerators.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610014072.0A CN105424671B (en) | 2016-01-11 | 2016-01-11 | A kind of preparation method of the suspending stabilized sample of frond for fluorescence measurement |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610014072.0A CN105424671B (en) | 2016-01-11 | 2016-01-11 | A kind of preparation method of the suspending stabilized sample of frond for fluorescence measurement |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105424671A true CN105424671A (en) | 2016-03-23 |
| CN105424671B CN105424671B (en) | 2018-06-19 |
Family
ID=55503020
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610014072.0A Active CN105424671B (en) | 2016-01-11 | 2016-01-11 | A kind of preparation method of the suspending stabilized sample of frond for fluorescence measurement |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105424671B (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101570785A (en) * | 2009-06-10 | 2009-11-04 | 南京大学 | Method for detecting potential inherent toxicity of organic pollutants in water body |
| CN103076297A (en) * | 2012-12-27 | 2013-05-01 | 河北科技大学 | Method for quickly and real-timely measuring water chlorophyll through replacing chlorophyll standard substance with microcystis aeruginosa |
| CN105044065A (en) * | 2015-08-07 | 2015-11-11 | 河北科技大学 | Preparation method for algae solution used for determining chlorophyll content through fluorescence method |
-
2016
- 2016-01-11 CN CN201610014072.0A patent/CN105424671B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101570785A (en) * | 2009-06-10 | 2009-11-04 | 南京大学 | Method for detecting potential inherent toxicity of organic pollutants in water body |
| CN103076297A (en) * | 2012-12-27 | 2013-05-01 | 河北科技大学 | Method for quickly and real-timely measuring water chlorophyll through replacing chlorophyll standard substance with microcystis aeruginosa |
| CN105044065A (en) * | 2015-08-07 | 2015-11-11 | 河北科技大学 | Preparation method for algae solution used for determining chlorophyll content through fluorescence method |
Non-Patent Citations (1)
| Title |
|---|
| 蒋建新: "《功能性多糖胶开发与应用》", 31 January 2013 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105424671B (en) | 2018-06-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Daley et al. | Direct counts of aquatic bacteria by a modified epifluorescence technique 1 | |
| Johan et al. | Laboratory measurement: Chlorophyll-a concentration measurement with acetone method using spectrophotometer | |
| Engel et al. | Effects of sea surface warming on the production and composition of dissolved organic matter during phytoplankton blooms: results from a mesocosm study | |
| Zhang et al. | Effects of long-term fertilization on soil humic acid composition and structure in Black Soil | |
| Zhao et al. | Nitrate assay for plant tissues | |
| Douglas et al. | Sedimentary environment influences ecosystem response to nutrient enrichment | |
| CN103175768B (en) | Fast detecting biological cell is fluorescent staining kit and the application thereof of state anyway | |
| CN108384539A (en) | A kind of green fluorescence carbon quantum dot, preparation method and applications | |
| CN105548064B (en) | The method for producing multiple nutritional components and titer of antibodies variation during antibiotic using near infrared ray microbial fermentation | |
| CN111253930B (en) | Fluorescent gold nanocluster and preparation method and application thereof | |
| Vasconcelos et al. | Role of polyphenols in copper complexation in red wines | |
| CN107271590A (en) | A kind of method of headspace gas chromatography quick detection anti-bacteria paper biocidal property | |
| Proctor et al. | Determination of inorganic phosphate in sea water by an iso-butanol extraction procedure | |
| Pausz et al. | Role of ultraviolet radiation on phytoplankton extracellular release and its subsequent utilization by marine bacterioplankton | |
| CN105044065A (en) | Preparation method for algae solution used for determining chlorophyll content through fluorescence method | |
| CN110243794A (en) | A kind of fluorescence probe for detecting sulfur dioxide and its application based on graphene quantum dot | |
| Hosseini et al. | A novel pH optical sensor using methyl orange based on triacetylcellulose membranes as support | |
| CN104597258A (en) | Method for detecting 17beta-estradiol by employing colorimetric method based on nucleic acid aptamer | |
| CN101586145A (en) | Analyzing method for detecting activity of soil xylanase | |
| CN105021605A (en) | Product and method used for detecting dissolved oxygen | |
| CN101586146B (en) | Analyzing method for detecting activity of soil xylanase | |
| CN103267748A (en) | Preparation method of microcystis aeruginosa liquid for determination of atrazine biotoxicity | |
| CN109852664B (en) | Method for detecting single algae biomass in water body of water bloom or red tide | |
| Ulusoy et al. | Micelle-mediated extraction and flame atomic absorption spectrometric method for determination of trace cobalt ions in beverage samples | |
| Vincent et al. | Photoadaptation to intermittent light across the St. Lawrence Estuary freshwater-saltwater transition zone |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |