CN105419932A - Extraction method of fish oil - Google Patents
Extraction method of fish oil Download PDFInfo
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- CN105419932A CN105419932A CN201510918548.9A CN201510918548A CN105419932A CN 105419932 A CN105419932 A CN 105419932A CN 201510918548 A CN201510918548 A CN 201510918548A CN 105419932 A CN105419932 A CN 105419932A
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/10—Production of fats or fatty oils from raw materials by extracting
- C11B1/106—Production of fats or fatty oils from raw materials by extracting using ultra-sounds
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/001—Refining fats or fatty oils by a combination of two or more of the means hereafter
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/008—Refining fats or fatty oils by filtration, e.g. including ultra filtration, dialysis
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/02—Refining fats or fatty oils by chemical reaction
- C11B3/04—Refining fats or fatty oils by chemical reaction with acids
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/02—Refining fats or fatty oils by chemical reaction
- C11B3/06—Refining fats or fatty oils by chemical reaction with bases
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/10—Refining fats or fatty oils by adsorption
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/16—Refining fats or fatty oils by mechanical means
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B5/00—Preserving by using additives, e.g. anti-oxidants
- C11B5/0021—Preserving by using additives, e.g. anti-oxidants containing oxygen
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Abstract
The invention discloses an extraction method of fish oil. The method at least comprises the following steps that 1, fish meat or fish viscera are smashed into meat paste, the meat paste is cooled rapidly and maintained for 10 min, then the cooled meat paste is melt at normal temperature, and the whole step is repeatedly conducted for 3-5 times; 2, water is added to the meat paste, a KOH solution is added, and ultrasonic waves are fed into the mixture; 3, centrifugation is conducted, and upper layer oily liquid is taken; 4, a citric acid solution is added to conduct acidizing treatment; 5, the KOH solution is added, stirring is conducted continuously, centrifugation is conducted, and upper layer oily liquid is taken; 6, activated carbon and activated carclazyte are added, constant-speed stirring is conducted for 20-30 min at 70-80 DEG C, and grease is separated through a filter press; 7, a mixture, with the volume being the same as that of the obtained grease, of ethyl alcohol and glycerol is added to the grease obtained in the sixth step, stirring is conducted to be uniform, distillation is conducted at 160-180 DEG C, and the fish oil is obtained. The extraction method of the fish oil has the advantages that the extraction rate of the fish oil is effectively increased, and the performance quality of the fish oil is effectively improved.
Description
Technical field
The present invention relates to a kind of method of purification, be specifically related to a kind of extracting method of fish oil.
Background technology
Fish oil is the general designation of the whole oily substances in fish body, it comprises body oil, liver oil and cerebrol, main fish oil is a kind of grease extracted from greasiness fish, and being rich in ω-3 is polyunsaturated fatty acid (DHA and EPA), has the health benefits such as anti-inflammatory, adjusting blood lipid.Sensu lato fish oil had both referred to the fish oil preparation of the forms such as capsule, referred to again the fat in fish body, and main function composition is ω-3 is wherein polyunsaturated fatty acid.
Existingly to prepare in the method for fish oil normal employing and adopt milling process, cooking process, solvent method or light alkali hydrolysis method to obtain crude fish oil more, but the method for above several method acquisition fish oil not that to yield poorly be exactly there is pollution.Add manufacturing cost virtually.In addition, in the process of refined fish oil, be easy to introduce foreign ion, cause fish oil to be polluted, be unfavorable for HUMAN HEALTH.
Therefore, a kind of output capacity extracting method that is high, that simultaneously do not introduce the fish oil of harmful ion becomes the key of dealing with problems.
Summary of the invention
As the result of various extensive and careful research and experiment, the present inventor has been found that: the flesh of fish is cooled to rapidly-180 ~-200 DEG C, it is made to melt at normal temperatures again, repeated multiple times, again supersound process is carried out to meat gruel, the weave construction of the flesh of fish can be destroyed, improve the stripping quantity of fish oil, and then the extraction yield of fish oil can be improved.
One object of the present invention is, provides a kind of extracting method of fish oil, can improve the stripping quantity of fish oil, and then can improve the extraction yield of fish oil.
For achieving the above object, the invention provides a kind of extracting method of fish oil, at least comprise the following steps:
A, the flesh of fish or internal organ smashed and makes meat gruel; Meat gruel is cooled to rapidly-180 ~-200 DEG C, keeps 10min, then make it melt at normal temperatures, 3 ~ 5 times repeatedly;
B, in the meat gruel after step a process, add the water of meat gruel weight 2 ~ 4 times, stir and make it to mix, then add KOH solution, adjust pH value to 7 ~ 8; Pass to ultrasonic wave to it again, 8 ~ 12KHz, process 30 ~ 180s, constantly stirs at 70 ~ 80 DEG C, keeps 30 ~ 60min;
C, after step b process, will carry out high speed centrifugation immediately, rotating speed is 4000 ~ 7000 turns; Get upper strata oily liquid;
D, add isopyknic water to gained oily liquid in step c, constantly stir and make it to mix, then add citric acid solution wherein and carry out acidification, and constantly stir, make solution PH remain on 5.5 ~ 6.5, stir 30 minutes;
E, in the solution after steps d process, add KOH solution, and constantly stir, adjustment solution Ph value to 7 ~ 8, centrifugal, get upper strata oily liquid;
Add the gac and atlapulgite that account for oily liquid weight 2% ~ 3% respectively in f, the oily liquid that obtains in step e, at 70 ~ 80 DEG C, at the uniform velocity stir 20 ~ 30min, be separated grease with pressure filter;
Add the mixture of isopyknic ethanol and glycerine in g, the grease that obtains in step f, stir, distill at 160 ~ 180 DEG C, obtain fish oil.
Preferably, the flesh of fish described in step a or internal organ be selected from mackerel, tuna, salmon, sturgeon, coilia, sardines, catfish, trout one or more.
Preferably, the volumetric molar concentration of the KOH solution described in step b is 1mol/L.
Preferably, the volumetric molar concentration of the citric acid solution described in steps d is 0.5mol/L.
Preferably, the volumetric molar concentration of the KOH solution described in step e is 1mol/L.
Preferably, in step f, add the superoxide-dismutase accounting for grease weight 0.2% ~ 0.3%.
Preferably, in the mixture of ethanol described in step g and glycerine, the volume ratio of ethanol and glycerine is 1: 1.
Preferably, the distillation temperature described in step g is 170 DEG C.
The invention has the beneficial effects as follows: the output that 1, effectively improve fish oil; 2, in the course of processing, add KOH solution but not NaOH solution, effectively reduce the existence of sodium salt in fish oil, reduce the harm of sodium salt to human body; 3, adopt citric acid solution to carry out acidification, improve the edible safety of fish oil.4, in decolorization, adopt gac and atlapulgite jointly to decolour, decolorizing effect is good, and adds superoxide-dismutase, effectively prevents fish oil to be oxidized, improves the colourity of fish oil; 5, distill after the mixture of fish oil and ethanol and glycerine being mixed, good deodorising effect can be obtained.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in further detail, can implement according to this with reference to specification sheets word to make those skilled in the art.
Embodiment 1
A, choose tuna the flesh of fish and internal organ, smashed and made meat gruel; Meat gruel is cooled to-190 DEG C rapidly, keeps 10min, then make it melt at normal temperatures, 4 times repeatedly;
B, in the meat gruel after step a process, add the water of meat gruel weight 3 times, stir and make it to mix, then add the KOH solution of 1mol/L, adjust pH value to 7.5; Pass to ultrasonic wave 10KHz to it again, process 120s, constantly stirs at 75 DEG C, keeps 45min;
C, after step b process, will carry out high speed centrifugation immediately, rotating speed is 6000 turns; Get upper strata oily liquid;
D, add isopyknic water to gained oily liquid in step c, constantly stir and make it to mix, then the citric acid solution adding 0.5mol/L wherein carries out acidification, and constantly stir, make solution PH remain on 6.0, stir 30 minutes;
E, in the solution after steps d process, add the KOH solution of 1mol/L, and constantly stir, adjustment solution Ph value to 7.5, centrifugal, get upper strata oily liquid;
Add the gac and atlapulgite that account for oily liquid weight 2.5% respectively in f, the oily liquid that obtains in step e, then add the superoxide-dismutase accounting for grease weight 0.25%, at 75 DEG C, at the uniform velocity stir 25min, be separated grease with pressure filter;
Add the mixture (wherein the volume ratio of ethanol and glycerine is 1: 1) of isopyknic ethanol and glycerine in g, the grease that obtains in step f, stir, distill at 170 DEG C, obtain fish oil.
Embodiment 2
A, choose salmon the flesh of fish or internal organ, smashed and made meat gruel; Meat gruel is cooled to-200 DEG C rapidly, keeps 10min, then make it melt at normal temperatures, 3 times repeatedly;
B, in the meat gruel after step a process, add the water of meat gruel weight 4 times, stir and make it to mix, then add the KOH solution of 1mol/L, adjust pH value to 7; Pass to ultrasonic wave to it again, 12KHz, process 30s, constantly stirs at 80 DEG C, keeps 30min;
C, after step b process, will carry out high speed centrifugation immediately, rotating speed is 7000 turns; Get upper strata oily liquid;
D, add isopyknic water to gained oily liquid in step c, constantly stir and make it to mix, then the citric acid solution adding 0.5mol/L wherein carries out acidification, and constantly stir, make solution PH remain on 6.5, stir 30 minutes;
E, in the solution after steps d process, add the KOH solution of 1mol/L, and constantly stir, adjustment solution Ph value to 7, centrifuging and taking upper strata oily liquid;
Add the gac and atlapulgite that account for oily liquid weight 3% respectively in f, the oily liquid that obtains in step e, then add the superoxide-dismutase accounting for grease weight 0.2%, at 70 DEG C, at the uniform velocity stir 30min, be separated grease with pressure filter;
Add the mixture (wherein the volume ratio of ethanol and glycerine is 1: 1) of isopyknic ethanol and glycerine in g, the grease that obtains in step f, stir, distill at 180 DEG C, obtain fish oil.
Embodiment 3
A, choose sardines the flesh of fish or internal organ, smashed and made meat gruel; Meat gruel is cooled to-180 DEG C rapidly, keeps 10min, then make it melt at normal temperatures, 5 times repeatedly;
B, in the meat gruel after step a process, add the water of meat gruel weight 2 times, stir and make it to mix, then add the KOH solution of 1mol/L, adjust pH value to 8; Pass to ultrasonic wave to it again, 8KHz, process 180s, constantly stirs at 70 DEG C, keeps 60min;
C, after step b process, will carry out high speed centrifugation immediately, rotating speed is 4000 turns; Get upper strata oily liquid;
D, add isopyknic water to gained oily liquid in step c, constantly stir and make it to mix, then the citric acid solution adding 0.5mol/L wherein carries out acidification, and constantly stir, make solution PH remain on 5.5, stir 30 minutes;
E, in the solution after steps d process, add the KOH solution of 1mol/L, and constantly stir, adjustment solution Ph value to 8, centrifuging and taking upper strata oily liquid;
Add the gac and atlapulgite that account for oily liquid weight 2% respectively in f, the oily liquid that obtains in step e, then add the superoxide-dismutase accounting for grease weight 0.3%, at 80 DEG C, at the uniform velocity stir 20min, be separated grease with pressure filter;
Add the mixture (wherein the volume ratio of ethanol and glycerine is 1: 1) of isopyknic ethanol and glycerine in g, the grease that obtains in step f, stir, distill at 160 DEG C, obtain fish oil.
Embodiment 4
A, choose catfish the flesh of fish or internal organ, smashed and made meat gruel; Meat gruel is cooled to-185 DEG C rapidly, keeps 10min, then make it melt at normal temperatures, 5 times repeatedly;
B, in the meat gruel after step a process, add the water of meat gruel weight 2.5 times, stir and make it to mix, then add the KOH solution of 1mol/L, adjust pH value to 7.2; Pass to ultrasonic wave to it again, 9KHz, process 60s, constantly stirs at 78 DEG C, keeps 50min;
C, after step b process, will carry out high speed centrifugation immediately, rotating speed is 4500 turns; Get upper strata oily liquid;
D, add isopyknic water to gained oily liquid in step c, constantly stir and make it to mix, then the citric acid solution adding 0.5mol/L wherein carries out acidification, and constantly stir, make solution PH remain on 6.2, stir 30 minutes;
E, in the solution after steps d process, add the KOH solution of 1mol/L, and constantly stir, adjustment solution Ph value to 7.8, centrifuging and taking upper strata oily liquid;
Add the gac and atlapulgite that account for oily liquid weight 2.4% respectively in f, the oily liquid that obtains in step e, then add the superoxide-dismutase accounting for grease weight 0.28%, at 80 DEG C, at the uniform velocity stir 23min, be separated grease with pressure filter;
Add the mixture (wherein the volume ratio of ethanol and glycerine is 1: 1) of isopyknic ethanol and glycerine in g, the grease that obtains in step f, stir, distill at 165 DEG C, obtain fish oil.
Embodiment 5
A, choose catfish the flesh of fish or internal organ, smashed and made meat gruel; Meat gruel is cooled to-195 DEG C rapidly, keeps 10min, then make it melt at normal temperatures, 3 times repeatedly;
B, in the meat gruel after step a process, add the water of meat gruel weight 3.5 times, stir and make it to mix, then add the KOH solution of 1mol/L, adjust pH value to 7.8; Pass to ultrasonic wave to it again, 11KHz, process 120s, constantly stirs at 74 DEG C, keeps 45min;
C, after step b process, will carry out high speed centrifugation immediately, rotating speed is 6500 turns; Get upper strata oily liquid;
D, add isopyknic water to gained oily liquid in step c, constantly stir and make it to mix, then the citric acid solution adding 0.5mol/L wherein carries out acidification, and constantly stir, make solution PH remain on 5.9, stir 30 minutes;
E, in the solution after steps d process, add the KOH solution of 1mol/L, and constantly stir, adjustment solution Ph value to 7.3, centrifuging and taking upper strata oily liquid;
Add the gac and atlapulgite that account for oily liquid weight 2.6% respectively in f, the oily liquid that obtains in step e, then add the superoxide-dismutase accounting for grease weight 0.26%, at 71 DEG C, at the uniform velocity stir 27min, be separated grease with pressure filter;
Add the mixture (wherein the volume ratio of ethanol and glycerine is 1: 1) of isopyknic ethanol and glycerine in g, the grease that obtains in step f, stir, distill at 166 DEG C, obtain fish oil.
Comparative example 1
The invention provides a kind of extracting method of fish oil, at least comprise the following steps:
A, choose tuna the flesh of fish and internal organ, smashed and made meat gruel;
B, in the meat gruel after step a process, add the water of meat gruel weight 3 times, stir and make it to mix, then add the NaOH solution of 1mol/L, adjust pH value to 7.5;
C, after step b process, will carry out high speed centrifugation immediately, rotating speed is 6000 turns; Get upper strata oily liquid;
D, add isopyknic water to gained oily liquid in step c, constantly stir and make it to mix, then the sulphuric acid soln adding 0.5mol/L wherein carries out acidification, and constantly stir, make solution PH remain on 6.0, stir 30 minutes;
E, in the solution after steps d process, add the NaOH solution of 1mol/L, and constantly stir, adjustment solution Ph value to 7.5, centrifugal, get upper strata oily liquid;
Add the gac and atlapulgite that account for oily liquid weight 2.5% respectively in f, the oily liquid that obtains in step e, at 75 DEG C, at the uniform velocity stir 25min, be separated grease with pressure filter;
Add isopyknic water in g, the grease that obtains in step f, stir, distill at 170 DEG C, obtain fish oil.
Comparative example 2
A, choose tuna the flesh of fish and internal organ, smashed and made meat gruel; Meat gruel is cooled to-190 DEG C rapidly, keeps 10min, then make it melt at normal temperatures, 4 times repeatedly;
B, in the meat gruel after step a process, add the water of meat gruel weight 3 times, stir and make it to mix, then add the NaOH solution of 1mol/L, adjust pH value to 7.5;
C, after step b process, will carry out high speed centrifugation immediately, rotating speed is 6000 turns; Get upper strata oily liquid;
D, add isopyknic water to gained oily liquid in step c, constantly stir and make it to mix, then the sulphuric acid soln adding 0.5mol/L wherein carries out acidification, and constantly stir, make solution PH remain on 6.0, stir 30 minutes;
E, in the solution after steps d process, add the NaOH solution of 1mol/L, and constantly stir, adjustment solution Ph value to 7.5, centrifugal, get upper strata oily liquid;
Add the gac and atlapulgite that account for oily liquid weight 2.5% respectively in f, the oily liquid that obtains in step e, at 75 DEG C, at the uniform velocity stir 25min, be separated grease with pressure filter;
Add isopyknic water in g, the grease that obtains in step f, stir, distill at 170 DEG C, obtain fish oil.
Comparative example 3
A, choose tuna the flesh of fish and internal organ, smashed and made meat gruel;
B, in the meat gruel after step a process, add the water of meat gruel weight 3 times, stir and make it to mix, then add the NaOH solution of 1mol/L, adjust pH value to 7.5; Pass to ultrasonic wave 10KHz to it again, process 120s, constantly stirs at 75 DEG C, keeps 45min;
C, after step b process, will carry out high speed centrifugation immediately, rotating speed is 6000 turns; Get upper strata oily liquid;
D, add isopyknic water to gained oily liquid in step c, constantly stir and make it to mix, then the sulphuric acid soln adding 0.5mol/L wherein carries out acidification, and constantly stir, make solution PH remain on 6.0, stir 30 minutes;
E, in the solution after steps d process, add the NaOH solution of 1mol/L, and constantly stir, adjustment solution Ph value to 7.5, centrifugal, get upper strata oily liquid;
Add the gac and atlapulgite that account for oily liquid weight 2.5% respectively in f, the oily liquid that obtains in step e, at 75 DEG C, at the uniform velocity stir 25min, be separated grease with pressure filter;
Add isopyknic water in g, the grease that obtains in step f, stir, distill at 170 DEG C, obtain fish oil.
Comparative example 4
A, choose tuna the flesh of fish and internal organ, smashed and made meat gruel;
B, in the meat gruel after step a process, add the water of meat gruel weight 3 times, stir and make it to mix, then add the KOH solution of 1mol/L, adjust pH value to 7.5;
C, after step b process, will carry out high speed centrifugation immediately, rotating speed is 6000 turns; Get upper strata oily liquid;
D, add isopyknic water to gained oily liquid in step c, constantly stir and make it to mix, then the sulphuric acid soln adding 0.5mol/L wherein carries out acidification, and constantly stir, make solution PH remain on 6.0, stir 30 minutes;
E, in the solution after steps d process, add the KOH solution of 1mol/L, and constantly stir, adjustment solution Ph value to 7.5, centrifugal, get upper strata oily liquid;
Add the gac accounting for oily liquid weight 5% in f, the oily liquid that obtains in step e, then add the superoxide-dismutase accounting for grease weight 0.25%, at 75 DEG C, at the uniform velocity stir 25min, be separated grease with pressure filter;
Add isopyknic water in g, the grease that obtains in step f, stir, distill at 170 DEG C, obtain fish oil.
Comparative example 5
A, choose tuna the flesh of fish and internal organ, smashed and made meat gruel;
B, in the meat gruel after step a process, add the water of meat gruel weight 3 times, stir and make it to mix, then add the NaOH solution of 1mol/L, adjust pH value to 7.5;
C, after step b process, will carry out high speed centrifugation immediately, rotating speed is 6000 turns; Get upper strata oily liquid;
D, add isopyknic water to gained oily liquid in step c, constantly stir and make it to mix, then the citric acid solution adding 0.5mol/L wherein carries out acidification, and constantly stir, make solution PH remain on 6.0, stir 30 minutes;
E, in the solution after steps d process, add the NaOH solution of 1mol/L, and constantly stir, adjustment solution Ph value to 7.5, centrifugal, get upper strata oily liquid;
Add the atlapulgite accounting for oily liquid weight 5% in f, the oily liquid that obtains in step e, at 75 DEG C, at the uniform velocity stir 25min, be separated grease with pressure filter;
Add the mixture (wherein the volume ratio of ethanol and glycerine is 1: 1) of isopyknic ethanol and glycerine in g, the grease that obtains in step f, stir, distill at 170 DEG C, obtain fish oil.
Data analysis
Respectively extraction yield, peroxide value, iodine value, acid value, detection whether containing sodium salt and sensory properties are carried out to the fish oil obtained in embodiment 1-5 and comparative example 1-5.Detected result is in table one:
Table one experimental data table
As shown in Table 1, whether embodiment 1-5 in extraction yield, peroxide value, iodine value, acid value, be all better than comparative example 1-5 containing the performance such as sodium salt and sensory properties, as can be seen here, method disclosed by the invention can effectively improve the extraction yield of fish oil, peroxide value, iodine value, acid value, whether containing the performance such as sodium salt and sensory properties.
As mentioned above, the extracting method of a kind of fish oil of the present invention, improves the extraction yield of fish oil effectively; In the course of processing, add KOH solution but not NaOH solution, effectively reduce the existence of sodium salt in fish oil, reduce the harm of sodium salt to human body; Adopt citric acid solution to carry out acidification, improve the edible safety of fish oil; In decolorization, adopt gac and atlapulgite jointly to decolour, decolorizing effect is good, and adds superoxide-dismutase, effectively prevents fish oil to be oxidized, improves the colourity of fish oil; Distill after the mixture of fish oil and ethanol and glycerine is mixed, good deodorising effect can be obtained.
Although embodiment of the present invention are open as above, but it is not restricted to listed in bright book and embodiment utilization, it can be applied to various applicable the field of the invention completely, for those skilled in the art, can easily realize other amendment, therefore do not deviating under the universal that claim and equivalency range limit, the present invention is not limited to specific details.
Claims (8)
1. an extracting method for fish oil, is characterized in that, at least comprises the following steps:
A, the flesh of fish or internal organ smashed and makes meat gruel; Meat gruel is cooled to rapidly-180 ~-200 DEG C, keeps 10min, then make it melt at normal temperatures, 3 ~ 5 times repeatedly;
B, in the meat gruel after step a process, add the water of meat gruel weight 2 ~ 4 times, stir and make it to mix, then add KOH solution, adjust pH value to 7 ~ 8; Pass to ultrasonic wave to it again, 8 ~ 12KHz, process 30 ~ 180s, constantly stirs at 70 ~ 80 DEG C, keeps 30 ~ 60min;
C, after step b process, will carry out high speed centrifugation immediately, rotating speed is 4000 ~ 7000 turns; Get upper strata oily liquid;
D, add isopyknic water to gained oily liquid in step c, constantly stir and make it to mix, then add citric acid solution wherein and carry out acidification, and constantly stir, make solution PH remain on 5.5 ~ 6.5, stir 30 minutes;
E, in the solution after steps d process, add KOH solution.And constantly stir, adjustment solution Ph value to 7 ~ 8, centrifugal, get upper strata oily liquid;
Add the gac and atlapulgite that account for oily liquid weight 2% ~ 3% respectively in f, the oily liquid that obtains in step e, at 70 ~ 80 DEG C, at the uniform velocity stir 20 ~ 30min, be separated grease with pressure filter;
Add the mixture of isopyknic ethanol and glycerine in g, the grease that obtains in step f, stir, distill at 160 ~ 180 DEG C, obtain fish oil.
2. the extracting method of fish oil as claimed in claim 1, wherein, the flesh of fish described in step a or internal organ be selected from mackerel, tuna, salmon, sturgeon, coilia, sardines, catfish, trout one or more.
3. the extracting method of fish oil as claimed in claim 2, wherein, the volumetric molar concentration of the KOH solution described in step b is 1mol/L.
4. the extracting method of fish oil as claimed in claim 1 or 2, wherein, the volumetric molar concentration of the citric acid solution described in steps d is 0.5mol/L.
5. the extracting method of fish oil as claimed in claim 4, wherein, the volumetric molar concentration of the KOH solution described in step e is 1mol/L.
6. the extracting method of fish oil as claimed in claim 1, wherein, adds the superoxide-dismutase accounting for grease weight 0.2% ~ 0.3% in step f.
7. the extracting method of fish oil as claimed in claim 1, wherein, in the mixture of ethanol described in step g and glycerine, the volume ratio of ethanol and glycerine is 1: 1.
8. the extracting method of fish oil as claimed in claim 1, wherein, the distillation temperature described in step g is 170 DEG C.
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