CN105410894B - A kind of preparation method of the anti-oxidant capsicum ferment of no alcohol - Google Patents
A kind of preparation method of the anti-oxidant capsicum ferment of no alcohol Download PDFInfo
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- 235000002566 Capsicum Nutrition 0.000 title claims abstract description 45
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- 230000003078 antioxidant effect Effects 0.000 title claims abstract description 16
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- 240000008574 Capsicum frutescens Species 0.000 title 1
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- 238000010438 heat treatment Methods 0.000 claims abstract description 9
- BOTDANWDWHJENH-UHFFFAOYSA-N Tetraethyl orthosilicate Chemical compound CCO[Si](OCC)(OCC)OCC BOTDANWDWHJENH-UHFFFAOYSA-N 0.000 claims abstract description 8
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 claims abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 34
- 238000010792 warming Methods 0.000 claims description 26
- 239000008367 deionised water Substances 0.000 claims description 19
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- 230000001954 sterilising effect Effects 0.000 claims description 18
- 239000000706 filtrate Substances 0.000 claims description 17
- 238000011282 treatment Methods 0.000 claims description 16
- 239000000203 mixture Substances 0.000 claims description 14
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- 102000004190 Enzymes Human genes 0.000 claims description 13
- 229940088598 enzyme Drugs 0.000 claims description 13
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 12
- 239000013078 crystal Substances 0.000 claims description 12
- 239000000243 solution Substances 0.000 claims description 9
- 230000015572 biosynthetic process Effects 0.000 claims description 7
- 238000003756 stirring Methods 0.000 claims description 7
- 238000003786 synthesis reaction Methods 0.000 claims description 7
- 229920001817 Agar Polymers 0.000 claims description 6
- 241000186018 Bifidobacterium adolescentis Species 0.000 claims description 6
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 claims description 6
- 240000001046 Lactobacillus acidophilus Species 0.000 claims description 6
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims description 6
- 229930195725 Mannitol Natural products 0.000 claims description 6
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 6
- 241000194020 Streptococcus thermophilus Species 0.000 claims description 6
- 239000008272 agar Substances 0.000 claims description 6
- 229910001593 boehmite Inorganic materials 0.000 claims description 6
- 239000012153 distilled water Substances 0.000 claims description 6
- 238000001704 evaporation Methods 0.000 claims description 6
- FAHBNUUHRFUEAI-UHFFFAOYSA-M hydroxidooxidoaluminium Chemical compound O[Al]=O FAHBNUUHRFUEAI-UHFFFAOYSA-M 0.000 claims description 6
- 238000011081 inoculation Methods 0.000 claims description 6
- 238000009413 insulation Methods 0.000 claims description 6
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims description 6
- 239000000594 mannitol Substances 0.000 claims description 6
- 235000010355 mannitol Nutrition 0.000 claims description 6
- 238000005360 mashing Methods 0.000 claims description 6
- 239000000843 powder Substances 0.000 claims description 6
- 230000001105 regulatory effect Effects 0.000 claims description 6
- 238000002791 soaking Methods 0.000 claims description 6
- 235000011121 sodium hydroxide Nutrition 0.000 claims description 6
- 239000006228 supernatant Substances 0.000 claims description 6
- 239000012137 tryptone Substances 0.000 claims description 6
- 108010059892 Cellulase Proteins 0.000 claims description 5
- 229940106157 cellulase Drugs 0.000 claims description 5
- 239000000701 coagulant Substances 0.000 claims description 5
- 229910001220 stainless steel Inorganic materials 0.000 claims description 5
- 239000010935 stainless steel Substances 0.000 claims description 5
- 238000004659 sterilization and disinfection Methods 0.000 claims description 5
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- 230000007062 hydrolysis Effects 0.000 claims description 4
- 238000006460 hydrolysis reaction Methods 0.000 claims description 4
- 238000005406 washing Methods 0.000 claims description 3
- 241000193830 Bacillus <bacterium> Species 0.000 claims 1
- 239000008267 milk Substances 0.000 claims 1
- 210000004080 milk Anatomy 0.000 claims 1
- 235000013336 milk Nutrition 0.000 claims 1
- 230000004151 fermentation Effects 0.000 abstract description 8
- 239000000463 material Substances 0.000 abstract description 6
- 230000003026 anti-oxygenic effect Effects 0.000 abstract description 4
- 150000001298 alcohols Chemical class 0.000 abstract description 3
- 239000000356 contaminant Substances 0.000 abstract description 2
- 230000009286 beneficial effect Effects 0.000 abstract 1
- 238000004140 cleaning Methods 0.000 abstract 1
- 238000001035 drying Methods 0.000 abstract 1
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 239000000047 product Substances 0.000 description 6
- 239000003643 water by type Substances 0.000 description 5
- 238000009835 boiling Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 208000024891 symptom Diseases 0.000 description 4
- 235000008534 Capsicum annuum var annuum Nutrition 0.000 description 3
- 240000008384 Capsicum annuum var. annuum Species 0.000 description 3
- 244000199885 Lactobacillus bulgaricus Species 0.000 description 3
- 235000013960 Lactobacillus bulgaricus Nutrition 0.000 description 3
- 229940004208 lactobacillus bulgaricus Drugs 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 241000186660 Lactobacillus Species 0.000 description 2
- 230000031709 bromination Effects 0.000 description 2
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- 125000000913 palmityl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 2
- 230000009759 skin aging Effects 0.000 description 2
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- 239000000499 gel Substances 0.000 description 1
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2220/00—Aspects relating to sorbent materials
- B01J2220/40—Aspects relating to the composition of sorbent or filter aid materials
- B01J2220/48—Sorbents characterised by the starting material used for their preparation
- B01J2220/4812—Sorbents characterised by the starting material used for their preparation the starting material being of organic character
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- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a kind of preparation method of the no anti-oxidant capsicum ferment of alcohol, belong to ferment preparation field.The ferment of the present invention is to hydrolyze that turbid must be mixed by material mixings such as tetraethyl orthosilicate, TPAOHs, gelled fluid is mixed to obtain with sodium hydroxide solution, cetyl trimethylammonium bromide, heating crystallization is stood, washs drying and roasting repeatedly, modified obtained fusel binding molecule sieve is standby；Take pimiento cleaning enzymolysis that enzymolysis liquid is made again, the probiotics on probiotic bacteria culture medium is seeded in capsicum enzymolysis liquid, anaerobic fermentation, when probiotics fermention produces ferment, it is effectively adsorbed by fusel binding molecule sieve, then it is centrifuged, is made without alcohol ferment.The beneficial effects of the invention are as follows：Preparation method of the present invention is simple, short without living contaminants, required fermentation time in preparation process；Products obtained therefrom steady quality is high without alcohols material, antioxygenic property.
Description
Technical field
The present invention relates to a kind of preparation method of the no anti-oxidant capsicum ferment of alcohol, belong to ferment preparation field.
Background technology
Ferment is one kind of comprehensive plant ferment, is to ferment to obtain by material of vegetable origins such as fruit and vegetables in ferment product
Ferment product.Ferment, also known as comprehensive plant organized enzyme, ferment are exactly being commonly called as " enzyme ".Start from earlier 1900s, nowadays untill
It is fashionable in American-European and Taiwan up to the history of more than 80 years.Ferment can strengthen human metabolism, remove incomplete in vivo
Metabolite, prompt activation physiological function, new cell is manufactured, be the optimal guarantee of intestinal health.Ferment is also beauty catalysis
Agent, its a variety of enzyme contained can suppress skin aging, participate in cuticula metabolism, repair wrinkle, delay skin aging speed.
Ferment mainly synthesizes in vivo, and the growth and pollution at age all can reduce internal ferment synthesis, and due to enzyme at high temperature
It can inactivate.The deficiency of human body endo enzyme can cause inferior health, aging acceleration, disease to produce, and for anti-aging, can manually extract
Ferment is taken as supplement.Ferment can adjust endotrophic equilibrium, plays antibacterial action, removes toxin, to keep the body of people to be good for
Health.Method prepares ferment using various fruits at present, and this fermentation process is easy to pollution microbes, and required fermentation time is grown,
Product ferment content after the completion of fermentation is low, it is easy to is inactivated by the digestion of stomach.And ferment product is most in Feedstock treating
Non-sterile sterilizing, fermented using the microorganism-tight of raw material institute's band in itself, cause unstable product quality, containing ethanol and its
His fusel, when serious, produces methanol during spontaneous fermentation, cause it is edible after be poisoned.Therefore work out a kind of ferment content it is high,
Without the high ferment production method of alcohol antioxygenic property, there is good development prospect in association area.
The content of the invention
The technical problems to be solved by the invention：Prepare ferment for current method, be also easy to produce in fermentation process ethanol and
Other fusels, when serious, methanol is produced during spontaneous fermentation, cause edible the drawbacks of being poisoned, there is provided one kind is without alcohol antioxygen
Change the preparation method of capsicum ferment, the present invention is modified by material mixings such as tetraethyl orthosilicate, TPAOHs and is made
Fusel binding molecule is sieved, and when probiotics fermention produces ferment, it is effectively adsorbed by fusel binding molecule sieve, then right
It is centrifuged, and is made without alcohol ferment.Gained ferment steady quality of the invention is high without alcohols material, antioxygenic property.
In order to solve the above technical problems, the present invention is using technical scheme as described below：
（1）Count in parts by weight, choose 20~45 parts of tetraethyl orthosilicates, 30~40 parts of TPAOHs, 10~
The boehmite of 20 parts of deionized waters and 15~20 part of 80~100 mesh, at 20~30 DEG C, stirred by 2500~3000r/min mixed
Close, it is hydrolyzed 3~4h, be prepared into mixing turbid, after the completion for the treatment of its hydrolysis, count in parts by weight, choose 55~80 parts respectively
Mix turbid, the sodium hydrate aqueous solution that 10~15 parts of mass concentrations are 10% and 10~30 parts of cetyl trimethyl brominations
Ammonium, mix 40~60min and be prepared into mixing gelled fluid；
（2）After coagulant liquid to be mixed stands 10~12h, it is transferred in stainless steel synthesis reactor, is heated to 100~110
DEG C, make 44~45h of its crystallization, after the completion for the treatment of crystallization, be washed with deionized 2~3 times, then with mass fraction be 10% hydrochloric acid
Solution wash 2~3 times, then by deionized water wash to pH be 7.0 after, by crystal be placed in 60~80 DEG C of baking oven dry 20
~24h, after the completion of to be dried, crystal is placed in Muffle furnace, 500 DEG C are warming up to by 5 DEG C/min speed program, then insulation
5~6h is calcined, taking-up naturally cools to room temperature and obtains fusel binding molecule sieve, standby；
（3）After fresh pimiento is cleaned, in mass ratio 1:10 mix capsicum with distilled water, are placed in 80~90 DEG C
25~30min of lower high-temperature heating, after the completion of heat soaking to be added, smashed to pieces with high-speed tissue mashing machine, then in mass ratio 1:25, will
Mixed enzymolysis liquid is stirred with capsicum mixed liquor, and being warming up to 50~55 DEG C makes it digest 110~120min, treats that enzymolysis is completed
Afterwards, 90~95 DEG C of 10~15min of destroy the enzyme treatment are continuously heating to, are then filtered enzymolysis liquid through 200 eye mesh screens, collect filter
It is standby that liquid obtains capsicum enzymolysis liquid；
（4）Count in parts by weight, choose 20~65 parts of mannitol, 5~15 parts of tryptone, 15~20 parts of yeast
With 20~45 parts of agar, it is stirred to form culture medium, it is 5.0~5.5 to add deionized water and wash to pH, complete pH to be regulated
Afterwards, 120 DEG C of 10~20min of sterilizing are warming up to it probiotic bacteria culture medium is made, then by inoculation ratio 1:1:1:1, by Bao Jiali
Sub- lactobacillus, streptococcus thermophilus, lactobacillus acidophilus, bifidobacterium adolescentis are seeded in probiotic bacteria culture medium, 36.5~37.5
12~16h of quiescent culture at DEG C；
（5）After standby capsicum enzymolysis liquid is warming up into 95~100 DEG C of 10~15min of heat sterilizations processing, by culture medium
On probiotics be seeded to sterilizing capsicum enzymolysis liquid in, anaerobic fermentation filters simultaneously after 2~3 days to it at 36.5~37.5 DEG C
Collect filtrate, then in mass ratio 1:10, by step（2）Obtained fusel binding molecule sieve is added in above-mentioned filtrate, and stirring is inhaled
Attached 5~6h, the zymotic fluid completed will be then adsorbed in 4800~5000r/min, 15~20min of centrifugation, collection supernatant liquor
And rotate to moisture evaporating completely, ferment powder is collected, produces one kind without the anti-oxidant capsicum ferment of alcohol.
Described step（3）Middle mixed enzymolysis liquid is by cellulase, pectase and deionized water in mass ratio 2:1:10
It is stirred to be formed.
The application process of the present invention：It will be prepared without the anti-oxidant capsicum ferment of alcohol, it is cold to be added to 30~40mL temperature
But into 40~45 DEG C of boiling water, dilute and be well mixed orally, twice daily, once take 8~10g, had rosy cheeks after taking,
It is energetic, no any discomfort symptom.
Compared with other method, advantageous effects are the present invention：
（1）Preparation method of the present invention is simple, short without living contaminants, required fermentation time in preparation process；
（2）Products obtained therefrom steady quality is high without alcohols material, antioxygenic property.
Embodiment
Count in parts by weight first, choose 20~45 parts of tetraethyl orthosilicates, 30~40 parts of TPAOHs, 10~
The boehmite of 20 parts of deionized waters and 15~20 part of 80~100 mesh, at 20~30 DEG C, stirred by 2500~3000r/min mixed
Close, it is hydrolyzed 3~4h, be prepared into mixing turbid, after the completion for the treatment of its hydrolysis, count in parts by weight, choose 55~80 parts respectively
Mix turbid, the sodium hydrate aqueous solution that 10~15 parts of mass concentrations are 10% and 10~30 parts of cetyl trimethyl brominations
Ammonium, mix 40~60min and be prepared into mixing gelled fluid；Then after coagulant liquid to be mixed stands 10~12h, it is transferred to stainless
In steel synthesis reactor, 100~110 DEG C are heated to, makes 44~45h of its crystallization, after the completion for the treatment of crystallization, is washed with deionized 2
~3 times, then with mass fraction be 10% hydrochloric acid solution wash 2~3 times, then by deionized water wash to pH be 7.0 after, general
Crystal, which is placed in 60~80 DEG C of baking oven, dries 20~24h, after the completion of to be dried, crystal is placed in Muffle furnace, by 5 DEG C/min
Speed program be warming up to 500 DEG C, then insulation roasting 5~6h, taking-up naturally cool to room temperature obtain fusel binding molecule sieve, it is standby
With；After fresh pimiento is cleaned again, in mass ratio 1:10 mix capsicum with distilled water, are placed in high temperature at 80~90 DEG C
25~30min is heated, after the completion of heat soaking to be added, is smashed to pieces with high-speed tissue mashing machine, then in mass ratio 1:25, by mixed enzyme
Solution liquid is stirred with capsicum mixed liquor, and being warming up to 50~55 DEG C makes it digest 110~120min, waits after the completion of digesting, and continues
90~95 DEG C of 10~15min of destroy the enzyme treatment are warming up to, are then filtered enzymolysis liquid through 200 eye mesh screens, collection filtrate obtains peppery
Green pepper enzymolysis liquid is standby；Count in parts by weight again, choose 20~65 parts mannitol, 5~15 parts of tryptone, 15~20 parts
Yeast and 20~45 parts of agar, are stirred to form culture medium, and it is 5.0~5.5 to add deionized water and wash to pH, to be regulated
After complete pH, 120 DEG C of 10~20min of sterilizing are warming up to it probiotic bacteria culture medium is made, then by inoculation ratio 1:1:1:1, it will protect
Leah lactobacillus, streptococcus thermophilus, lactobacillus acidophilus, bifidobacterium adolescentis is added to be seeded in probiotic bacteria culture medium, 36.5~
12~16h of quiescent culture at 37.5 DEG C；Finally by standby capsicum enzymolysis liquid be warming up to 95~100 DEG C of heat sterilizations processing 10~
After 15min, the probiotics on culture medium is seeded in sterilizing capsicum enzymolysis liquid, the anaerobic fermentation 2~3 at 36.5~37.5 DEG C
After it, it is filtered and collects filtrate, then in mass ratio 1:10, standby fusel binding molecule sieve is added into above-mentioned filtrate
In, 5~6h of stirring and adsorbing, the zymotic fluid completed will be then adsorbed in 4800~5000r/min, 15~20min of centrifugation, receipts
Collect supernatant liquor and rotate to moisture evaporating completely, collect ferment powder, produce one kind without the anti-oxidant capsicum ferment of alcohol；Described
Mixed enzymolysis liquid is by cellulase, pectase and deionized water in mass ratio 2:1:10 are stirred to be formed.
Example 1
Count in parts by weight first, choose 20 parts of tetraethyl orthosilicates, 40 parts of TPAOHs, 20 parts of deionized waters
With the boehmite of 20 part of 80 mesh, at 20 DEG C, it is stirred by 2500r/min, it is hydrolyzed 3h, be prepared into mixing turbid, treat
After the completion of it is hydrolyzed, count in parts by weight, choose 80 parts of mixing turbids respectively, the sodium hydroxide water that 10 parts of mass concentrations are 10%
Solution and 10 parts of cetyl trimethylammonium bromides, mix 40min and be prepared into mixing gelled fluid；Then coagulant liquid to be mixed
After standing 10h, it is transferred in stainless steel synthesis reactor, is heated to 100 DEG C, make its crystallization 44h, after the completion for the treatment of crystallization, spend
Ion water washing 2 times, then washed 2 times with the hydrochloric acid solution that mass fraction is 10%, then it is 7.0 to be washed by deionized water to pH
Afterwards, crystal is placed in 60 DEG C of baking oven and dries 20h, after the completion of to be dried, crystal is placed in Muffle furnace, by 5 DEG C/min's
Speed program is warming up to 500 DEG C, then insulation roasting 5h, and taking-up naturally cools to room temperature and obtains fusel binding molecule sieve, standby；Again
After fresh pimiento is cleaned, in mass ratio 1:10 mix capsicum with distilled water, are placed in being heated at high temperature at 80 DEG C
25min, after the completion of heat soaking to be added, smashed to pieces with high-speed tissue mashing machine, then in mass ratio 1:25, by mixed enzymolysis liquid with it is peppery
Green pepper mixed liquor is stirred, and being warming up to 50 DEG C makes it digest 110min, waits after the completion of digesting, is continuously heating to 90 DEG C of destroy the enzyme treatments
10min, then enzymolysis liquid is filtered through 200 eye mesh screens, it is standby that collection filtrate obtains capsicum enzymolysis liquid；Again in parts by weight
Meters, 60 parts of mannitol, 5 parts of tryptone, 15 parts of yeast and 20 parts of agar are chosen, is stirred to form culture medium,
It is 5.0 to add deionized water and wash to pH, after complete pH to be regulated, 120 DEG C of sterilizing 10min is warming up to it probiotic's culture is made
Base, then by inoculation ratio 1:1:1:1, lactobacillus bulgaricus, streptococcus thermophilus, lactobacillus acidophilus, bifidobacterium adolescentis are connect
Kind is into probiotic bacteria culture medium, the quiescent culture 12h at 36.5 DEG C；Standby capsicum enzymolysis liquid is finally warming up to 95 DEG C of heating
After sterilization treatment 10min, the probiotics on culture medium is seeded in sterilizing capsicum enzymolysis liquid, the anaerobic fermentation 2 at 36.5 DEG C
After it, it is filtered and collects filtrate, then in mass ratio 1:10, standby fusel binding molecule sieve is added into above-mentioned filtrate
In, stirring and adsorbing 5h, the zymotic fluid for adsorbing completion is then centrifuged into 15min in 4800r/min, collect supernatant liquor and simultaneously revolve
Moisture evaporating completely is gone to, collects ferment powder, produces one kind without the anti-oxidant capsicum ferment of alcohol；Described mixed enzymolysis liquid be by
Cellulase, pectase and deionized water in mass ratio 2:1:10 are stirred to be formed.
It will be prepared without the anti-oxidant capsicum ferment of alcohol, be added to 30mL temperature and be cooled in 40 DEG C of boiling water, dilute
It is well mixed oral, twice daily, 8g is once taken, had rosy cheeks after taking, be energetic, no any discomfort symptom.
Example 2
Count in parts by weight first, choose 45 parts of tetraethyl orthosilicates, 30 parts of TPAOHs, 10 parts of deionized waters
With the boehmite of 15 part of 90 mesh, at 25 DEG C, it is stirred by 2800r/min, it is hydrolyzed 3.5h, be prepared into mixing turbid,
After the completion for the treatment of its hydrolysis, count in parts by weight, choose 55 parts of mixing turbids respectively, the sodium hydroxide that 15 parts of mass concentrations are 10%
The aqueous solution and 30 parts of cetyl trimethylammonium bromides, mix 50min and be prepared into mixing gelled fluid；Then gel to be mixed
After liquid stands 11h, it is transferred in stainless steel synthesis reactor, is heated to 105 DEG C, make its crystallization 44.5h, after the completion for the treatment of crystallization,
It is washed with deionized 3 times, then is washed 3 times with the hydrochloric acid solution that mass fraction is 10%, then is washed by deionized water to pH
After 7.0, by crystal be placed in 70 DEG C of baking oven dry 22h, after the completion of to be dried, crystal is placed in Muffle furnace, by 5 DEG C/
Min speed program is warming up to 500 DEG C, then insulation roasting 5.5h, and taking-up naturally cools to room temperature and obtains fusel binding molecule sieve,
It is standby；After fresh pimiento is cleaned again, in mass ratio 1:10 mix capsicum with distilled water, are placed in high temperature at 85 DEG C and add
Hot 28min, after the completion of heat soaking to be added, smashed to pieces with high-speed tissue mashing machine, then in mass ratio 1:25, by mixed enzymolysis liquid with
Capsicum mixed liquor is stirred, and being warming up to 53 DEG C makes it digest 115min, waits after the completion of digesting, is continuously heating at 93 DEG C of enzyme deactivations
12min is managed, is then filtered enzymolysis liquid through 200 eye mesh screens, it is standby that collection filtrate obtains capsicum enzymolysis liquid；Again in parts by weight
Meters, 20 parts of mannitol, 15 parts of tryptone, 20 parts of yeast and 45 parts of agar are chosen, is stirred to form culture medium,
It is 5.3 to add deionized water and wash to pH, after complete pH to be regulated, 120 DEG C of sterilizing 15min is warming up to it probiotic's culture is made
Base, then by inoculation ratio 1:1:1:1, lactobacillus bulgaricus, streptococcus thermophilus, lactobacillus acidophilus, bifidobacterium adolescentis are connect
Kind is into probiotic bacteria culture medium, the quiescent culture 14h at 37 DEG C；Standby capsicum enzymolysis liquid finally is warming up into 98 DEG C of heating to go out
After bacterium processing 13min, the probiotics on culture medium is seeded in sterilizing capsicum enzymolysis liquid, the anaerobic fermentation after 3 days at 37 DEG C,
It is filtered and collects filtrate, then in mass ratio 1:10, standby fusel binding molecule sieve is added in above-mentioned filtrate, stirring
5h is adsorbed, the zymotic fluid for adsorbing completion is then centrifuged into 15min in 4800r/min, supernatant liquor is collected and simultaneously rotates to water
Divide evaporating completely, collect ferment powder, produce one kind without the anti-oxidant capsicum ferment of alcohol；Described mixed enzymolysis liquid is by cellulose
Enzyme, pectase and deionized water in mass ratio 2:1:10 are stirred to be formed.
It will be prepared without the anti-oxidant capsicum ferment of alcohol, be added to 35mL temperature and be cooled in 42 DEG C of boiling water, dilute
It is well mixed oral, twice daily, 9g is once taken, had rosy cheeks after taking, be energetic, no any discomfort symptom.
Example 3
Count in parts by weight first, choose 30 parts of tetraethyl orthosilicates, 35 parts of TPAOHs, 17 parts of deionized waters
With the boehmite of 18 part of 100 mesh, at 30 DEG C, it is stirred by 3000r/min, it is hydrolyzed 4h, be prepared into mixing turbid, treat
After the completion of it is hydrolyzed, count in parts by weight, choose 60 parts of mixing turbids respectively, the sodium hydroxide water that 13 parts of mass concentrations are 10%
Solution and 27 parts of cetyl trimethylammonium bromides, mix 60min and be prepared into mixing gelled fluid；Then coagulant liquid to be mixed
After standing 12h, it is transferred in stainless steel synthesis reactor, is heated to 110 DEG C, make its crystallization 45h, after the completion for the treatment of crystallization, spend
Ion water washing 3 times, then washed 3 times with the hydrochloric acid solution that mass fraction is 10%, then it is 7.0 to be washed by deionized water to pH
Afterwards, crystal is placed in 80 DEG C of baking oven and dries 24h, after the completion of to be dried, crystal is placed in Muffle furnace, by 5 DEG C/min's
Speed program is warming up to 500 DEG C, then insulation roasting 6h, and taking-up naturally cools to room temperature and obtains fusel binding molecule sieve, standby；Again
After fresh pimiento is cleaned, in mass ratio 1:10 mix capsicum with distilled water, are placed in being heated at high temperature at 90 DEG C
30min, after the completion of heat soaking to be added, smashed to pieces with high-speed tissue mashing machine, then in mass ratio 1:25, by mixed enzymolysis liquid with it is peppery
Green pepper mixed liquor is stirred, and being warming up to 55 DEG C makes it digest 120min, waits after the completion of digesting, is continuously heating to 95 DEG C of destroy the enzyme treatments
15min, then enzymolysis liquid is filtered through 200 eye mesh screens, it is standby that collection filtrate obtains capsicum enzymolysis liquid；Again in parts by weight
Meters, 35 parts of mannitol, 10 parts of tryptone, 18 parts of yeast and 37 parts of agar are chosen, is stirred to form culture medium,
It is 5.5 to add deionized water and wash to pH, after complete pH to be regulated, 120 DEG C of sterilizing 20min is warming up to it probiotic's culture is made
Base, then by inoculation ratio 1:1:1:1, lactobacillus bulgaricus, streptococcus thermophilus, lactobacillus acidophilus, bifidobacterium adolescentis are connect
Kind is into probiotic bacteria culture medium, the quiescent culture 16h at 37.5 DEG C；Standby capsicum enzymolysis liquid finally is warming up into 100 DEG C to add
After heat sterilization processing 15min, the probiotics on culture medium is seeded in sterilizing capsicum enzymolysis liquid, the anaerobic fermentation at 37.5 DEG C
After 3 days, it is filtered and collects filtrate, then in mass ratio 1:10, standby fusel binding molecule sieve is added into above-mentioned filtrate
In, stirring and adsorbing 6h, the zymotic fluid for adsorbing completion is then centrifuged into 20min in 5000r/min, collect supernatant liquor and simultaneously revolve
Moisture evaporating completely is gone to, collects ferment powder, produces one kind without the anti-oxidant capsicum ferment of alcohol；Described mixed enzymolysis liquid be by
Cellulase, pectase and deionized water in mass ratio 2:1:10 are stirred to be formed.
It will be prepared without the anti-oxidant capsicum ferment of alcohol, be added to 40mL temperature and be cooled in 45 DEG C of boiling water, dilute
It is well mixed oral, twice daily, 10g is once taken, had rosy cheeks after taking, be energetic, no any discomfort symptom.
Claims (2)
1. a kind of preparation method of the anti-oxidant capsicum ferment of no alcohol, it is characterised in that specifically preparation process is：
（1）Count in parts by weight, choose 20~45 parts of tetraethyl orthosilicates, 30~40 parts of TPAOHs, 10~20 parts
The boehmite of deionized water and 15~20 part of 80~100 mesh, at 20~30 DEG C, it is stirred, makes by 2500~3000r/min
It hydrolyzes 3~4h, is prepared into mixing turbid, after the completion for the treatment of its hydrolysis, counts in parts by weight, and it is turbid to choose 55~80 parts of mixing respectively
The sodium hydrate aqueous solution and 10~30 parts of cetyl trimethylammonium bromides that liquid, 10~15 parts of mass concentrations are 10%, mixing
40~60min of stirring is prepared into mixing gelled fluid；
（2）After coagulant liquid to be mixed stands 10~12h, it is transferred in stainless steel synthesis reactor, is heated to 100~110 DEG C, makes
44~45h of its crystallization, after the completion for the treatment of crystallization, be washed with deionized 2~3 times, then with mass fraction be 10% hydrochloric acid solution
Washing 2~3 times, then by deionized water wash to pH be 7.0 after, by crystal be placed in 60~80 DEG C of baking oven dry 20~
24h, after the completion of to be dried, crystal is placed in Muffle furnace, 500 DEG C are warming up to by 5 DEG C/min speed program, then insulation roasting
5~6h is burnt, taking-up naturally cools to room temperature and obtains fusel binding molecule sieve, standby；
（3）After fresh pimiento is cleaned, in mass ratio 1:10 mix capsicum with distilled water, are placed at 80~90 DEG C high
Temperature 25~30min of heating, after the completion of heat soaking to be added, is smashed to pieces with high-speed tissue mashing machine, and then in mass ratio 1:25, it will mix
Enzymolysis liquid is stirred with capsicum mixed liquor, and being warming up to 50~55 DEG C makes it digest 110~120min, waits after the completion of digesting, after
It is continuous to be warming up to 90~95 DEG C of 10~15min of destroy the enzyme treatment, then enzymolysis liquid is filtered through 200 eye mesh screens, filtrate is collected and obtains
Capsicum enzymolysis liquid is standby；
（4）Count in parts by weight, choose 20~65 parts of mannitol, 5~15 parts of tryptone, 15~20 parts of yeast and 20
~45 parts of agar, it is stirred to form culture medium, it is 5.0~5.5 to add deionized water and wash to pH, after complete pH to be regulated,
120 DEG C of 10~20min of sterilizing are warming up to it probiotic bacteria culture medium is made, then by inoculation ratio 1:1:1:1, by bulgarian milk
Bacillus, streptococcus thermophilus, lactobacillus acidophilus, bifidobacterium adolescentis are seeded in probiotic bacteria culture medium, at 36.5~37.5 DEG C
12~16h of quiescent culture；
（5）After standby capsicum enzymolysis liquid is warming up into 95~100 DEG C of 10~15min of heat sterilizations processing, by culture medium
Probiotics is seeded in sterilizing capsicum enzymolysis liquid, and anaerobic fermentation was filtered and collected to it after 2~3 days at 36.5~37.5 DEG C
Filtrate, then in mass ratio 1:10, by step（2）Obtained fusel binding molecule sieve is added in above-mentioned filtrate, and stirring and adsorbing 5~
6h, the zymotic fluid for adsorbing completion is then centrifuged into 15~20min in 4800~5000r/min, collect supernatant liquor and simultaneously revolve
Moisture evaporating completely is gone to, collects ferment powder, produces one kind without the anti-oxidant capsicum ferment of alcohol.
A kind of 2. preparation method of no anti-oxidant capsicum ferment of alcohol according to claim 1, it is characterised in that：Described is mixed
It is by cellulase, pectase and deionized water in mass ratio 2 to close enzymolysis liquid:1:10 are stirred to be formed.
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| CN101629133A (en) * | 2009-05-02 | 2010-01-20 | 滕传文 | Beverage wine brewed by combining liquid state complete fermentation and solid state fermentation distillation |
| CN103073915A (en) * | 2013-02-07 | 2013-05-01 | 湖南威嘉生物科技有限公司 | Process for extracting and separating capsanthin and capsaicin by using biological enzyme |
| CN103621977A (en) * | 2013-12-17 | 2014-03-12 | 宁夏红川农业科技有限公司 | Fermented chilli sauce |
| JP5592583B1 (en) * | 2014-03-24 | 2014-09-17 | 佐太郎 登川 | Feed or health food and method for producing the same |
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| CN101629133A (en) * | 2009-05-02 | 2010-01-20 | 滕传文 | Beverage wine brewed by combining liquid state complete fermentation and solid state fermentation distillation |
| CN103073915A (en) * | 2013-02-07 | 2013-05-01 | 湖南威嘉生物科技有限公司 | Process for extracting and separating capsanthin and capsaicin by using biological enzyme |
| CN103621977A (en) * | 2013-12-17 | 2014-03-12 | 宁夏红川农业科技有限公司 | Fermented chilli sauce |
| JP5592583B1 (en) * | 2014-03-24 | 2014-09-17 | 佐太郎 登川 | Feed or health food and method for producing the same |
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