CN105400852B - A method of neomycin is produced using amino acid fermentation bacteria residue substitution fermentation raw material - Google Patents
A method of neomycin is produced using amino acid fermentation bacteria residue substitution fermentation raw material Download PDFInfo
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- CN105400852B CN105400852B CN201510991909.2A CN201510991909A CN105400852B CN 105400852 B CN105400852 B CN 105400852B CN 201510991909 A CN201510991909 A CN 201510991909A CN 105400852 B CN105400852 B CN 105400852B
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- amino acid
- bacteria residue
- neomycin
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- acid fermentation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/44—Preparation of O-glycosides, e.g. glucosides
- C12P19/46—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical bound to a cyclohexyl radical, e.g. kasugamycin
- C12P19/48—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical bound to a cyclohexyl radical, e.g. kasugamycin the cyclohexyl radical being substituted by two or more nitrogen atoms, e.g. destomycin, neamin
- C12P19/50—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical bound to a cyclohexyl radical, e.g. kasugamycin the cyclohexyl radical being substituted by two or more nitrogen atoms, e.g. destomycin, neamin having two saccharide radicals bound through only oxygen to adjacent ring carbon atoms of the cyclohexyl radical, e.g. ambutyrosin, ribostamycin
- C12P19/52—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical bound to a cyclohexyl radical, e.g. kasugamycin the cyclohexyl radical being substituted by two or more nitrogen atoms, e.g. destomycin, neamin having two saccharide radicals bound through only oxygen to adjacent ring carbon atoms of the cyclohexyl radical, e.g. ambutyrosin, ribostamycin containing three or more saccharide radicals, e.g. neomycin, lividomycin
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Abstract
A method of neomycin being produced using amino acid fermentation bacteria residue substitution fermentation raw material, method includes the following steps: 1) preparing amino acid fermentation bacteria residue: amino acid fermentation bacteria residue is made in the waste water after amino acids production after primary filtration；2) by amino acid fermentation bacteria residue made from step 1) as the ingredient of the fermentation medium in neomycin fermenting and producing, part is substituted or replacing whole soybean powder is as fermentation raw material production neomycin.A kind of method using amino acid fermentation bacteria residue substitution fermentation raw material production neomycin provided by the invention, it can solve the high bacteria residue processing cost generated after amino acid production by ferment method, pollution environment, neomycin fermenting and producing fermentation raw material problem at high cost, make the waste utilization of amino acid fermentation bacteria residue and reduce neomycin fermenting and producing cost, the performance of enterprises greatly improves.
Description
Technical field
It is especially a kind of using the substitution fermentation raw material production of amino acid fermentation bacteria residue the present invention relates to neomycin production field
The method of neomycin.
Background technique
Production strain is inoculated on culture medium through multistage purebred training by fermentation industry mostly using agricultural and sideline product resource as raw material
It supports, final production goes out the target products such as amino acid, antibiotic, therefore after target product extracts, the yield of waste water is larger, adds
A large amount of solid matrix is had in upper production process to be discharged into waste water, brings pressure to environmental protection of enterprise.Currently, correlation section of China
It grinds universities and colleges, amino acids production enterprise and has done a large amount of work around the treatment process of amino acid wastewater and comprehensive utilization, propose
Many new treatment process and resource utilization scheme, improvements of amino acid wastewater be just gradually intended to new treatment process with entirely
Process resourcesization are using the Comprehensive Management Model that combines, to finally on the basis of solving water pollution, maximum resource
Using amino acid fermentation waste liquid, good economic benefit and social benefit are created.
The solid content that waste water in amino acids production obtains after primary filtration, i.e. amino acid bacteria residue, main component are bacterium
Filament, the degradation product of the culture medium not utilized and the metabolite, culture medium that are generated in fermentation process, in extraction process
Flocculant, acidulant of addition etc., wherein containing many valuable resources: the solid matters such as amino acid thallus, protein suspend
Object, plurality of inorganic salt, organic acid and reduced sugar etc., domestic enterprise mainly has using more resource utilization method at present: mentioning
Compound organic and inorganic fertilizer is produced after taking mycoprotein；Produce fodder yeast albumen after, recycle ammonium sulfate production organic and inorganic fertilizer and
Livestock fodder additives etc. are produced, there are also certain methods production biological pesticide, production biological flocculants etc..Its processing mode is mostly
It needs by filtering, concentration, drying (or spontaneously dry), therefore although bacteria residue brings certain economy as by-product
Benefit, but after comprehensively considering its processing cost, produce little effect in Business Economic Benefit promotion, it is even unable to make ends meet, it is right
In enterprise usually only under the pressure of the thing for mitigating environmental protection pressure and being compelled to do.It need to seek new amino acid bacteria residue processing way
Diameter mitigates the environmental protection pressure of enterprise, and then promotes Business Economic Benefit.
Summary of the invention
Technical problem to be solved by the invention is to provide a kind of using the substitution fermentation raw material production of amino acid fermentation bacteria residue
The method of neomycin can solve the high bacteria residue processing cost generated after amino acid production by ferment method, pollution environment, neomycin fermentation
Fermentation raw material problem at high cost is produced, the waste utilization of amino acid fermentation bacteria residue is made and reduces neomycin fermenting and producing cost,
The performance of enterprises greatly improves.
In order to solve the above technical problems, the technical scheme adopted by the invention is that: it is a kind of to be replaced using amino acid fermentation bacteria residue
For the method for fermentation raw material production neomycin, method includes the following steps:
1) it prepares amino acid fermentation bacteria residue: amino acid fermentation bacterium is made in the waste water after amino acids production after primary filtration
Slag；
2) by amino acid fermentation bacteria residue made from step 1) as the ingredient of the fermentation medium in neomycin fermenting and producing,
Part substitution or replacing whole soybean powder are as fermentation raw material production neomycin.
In step 1), the waste water after amino acids production is carried out by primary filtration using ceramic membrane.
Amino acid fermentation bacteria residue made from step 1) is detected, 10% ammonia is not less than using dry biomass ratio
Base acid bacteria residue, total nitrogen analysis knot fruit≤45% in amino acid bacteria residue.
In step 2), the mass ratio that amino acid fermentation bacteria residue substitutes soybean powder is the 50-100% of soybean powder quality.
The each component parts by weight of fermentation medium are as follows in step 2):
A kind of method using amino acid fermentation bacteria residue substitution fermentation raw material production neomycin provided by the invention, beneficial to effect
Fruit is as follows:
1, it with the soybean powder in amino acid fermentation bacteria residue substitution fermentation raw material, is generated as neomycin fermentation nitrogen source direct
Economic benefit is as follows:
As can be seen from the above table, neomycin fermentation raw material cost is calculated with 2014 yearly plan valences, amino acid bacteria residue all replaces
After soybean powder, fermentating formula cost decline 38%, although above calculate does not take into account bacteria residue and transportation cost, its
The environmental protection treatment cost of bacteria residue is not eaten up part of similarly, therefore, after doing fermentation raw material using amino acid bacteria residue substitution soybean powder,
Neomycin fermentating formula cost declines to a great extent.
2, amino acid fermentation residue, environmental protection treatment pressure is big, and the processing that needs to inject capital into, and after present simple filtration, does
For neomycin fermentation nitrogen source, substitution soybean powder completely can both be returned as the new way of the resource utilization to amino acid bacteria residue
A large amount of utilities are received, the waste strength generated in production process can also be made to substantially reduce, mitigate next step sewage treatment
Burden, obtains good environmental benefit and social benefit.
Specific embodiment
Embodiment one
A method of neomycin being produced using amino acid fermentation bacteria residue substitution fermentation raw material, this method includes following step
It is rapid:
1) it prepares amino acid fermentation bacteria residue: amino acid fermentation bacterium is made in the waste water after amino acids production after primary filtration
Slag；
2) by amino acid fermentation bacteria residue made from step 1) as the ingredient of the fermentation medium in neomycin fermenting and producing,
Part substitution or replacing whole soybean powder are as fermentation raw material production neomycin.
In step 1), the waste water after amino acids production is carried out by primary filtration using ceramic membrane.
Amino acid fermentation bacteria residue made from step 1) is detected, 10% ammonia is not less than using dry biomass ratio
Base acid bacteria residue, total nitrogen analysis knot fruit≤45% in amino acid bacteria residue.
In step 2), the mass ratio that amino acid fermentation bacteria residue substitutes soybean powder is the 50% of soybean powder quality.
The each component of fermentation medium is as follows in step 2):
Embodiment two
A method of neomycin being produced using amino acid fermentation bacteria residue substitution fermentation raw material, this method includes following step
It is rapid:
1) it prepares amino acid fermentation bacteria residue: amino acid fermentation bacterium is made in the waste water after amino acids production after primary filtration
Slag；
2) by amino acid fermentation bacteria residue made from step 1) as the ingredient of the fermentation medium in neomycin fermenting and producing,
Part substitution or replacing whole soybean powder are as fermentation raw material production neomycin.
In step 1), the waste water after amino acids production is carried out by primary filtration using ceramic membrane.
Amino acid fermentation bacteria residue made from step 1) is detected, 10% ammonia is not less than using dry biomass ratio
Base acid bacteria residue, total nitrogen analysis knot fruit≤45% in amino acid bacteria residue.
In step 2), the mass ratio that amino acid fermentation bacteria residue substitutes soybean powder is the 75% of soybean powder quality.
The each component of fermentation medium is as follows in step 2):
Embodiment three
Embodiment one
A method of neomycin being produced using amino acid fermentation bacteria residue substitution fermentation raw material, this method includes following step
It is rapid:
1) it prepares amino acid fermentation bacteria residue: amino acid fermentation bacterium is made in the waste water after amino acids production after primary filtration
Slag；
2) by amino acid fermentation bacteria residue made from step 1) as the ingredient of the fermentation medium in neomycin fermenting and producing,
Part substitution or replacing whole soybean powder are as fermentation raw material production neomycin.
In step 1), the waste water after amino acids production is carried out by primary filtration using ceramic membrane.
Amino acid fermentation bacteria residue made from step 1) is detected, 10% ammonia is not less than using dry biomass ratio
Base acid bacteria residue, total nitrogen analysis knot fruit≤45% in amino acid bacteria residue.
In step 2), the mass ratio that amino acid fermentation bacteria residue substitutes soybean powder is the 100% of soybean powder quality.
The each component of fermentation medium is as follows in step 2):
Example IV
Amino acid bacteria residue substitutes soybean powder fermenting and producing neomycinsulphate Experimental Comparison
| It ferments duration (hour) | Put tank potency (U/mL) | Component C | |
| Control group | 156 | 16913 | Meet regulation |
| Test group first (50% substitution) | 156 | 17658 | Meet regulation |
| Test group second batch (50% substitution) | 156 | 17191 | Meet regulation |
| Test group third batch (50% substitution) | 156 | 17215 | Meet regulation |
| Test group the 4th batch (75% substitution) | 156 | 17012 | Meet regulation |
| Experimental group the 5th batch (100% substitution) | 159 | 16977 | Meet regulation |
Embodiment five
Fermentation process is the prior art, and key step is as follows:
1) sand spore is sealed, it is dry, slant pore suspension is made after culture；
2) slant pore suspension is put into the seeding tank for preset seed culture medium at a temperature of 35 degrees Celsius
Culture obtains culture solution in 32~40 hours；
3) seed culture fluid obtained through step 2) is sent into preset be inoculated in the fermentor of fermentation medium and in
It ferments 140~160 hours at a temperature of 35 degrees Celsius and obtains fermentation liquid；
4) culture for terminating fermentation liquid, is put into adsorption tanks for fermentation liquid, i.e. completion fermentation step.
Wherein, seed culture medium and fermentation medium are all made of the technical solution of embodiment one-three.
Claims (3)
1. a kind of method using amino acid fermentation bacteria residue substitution fermentation raw material production neomycin, it is characterised in that this method includes
Following steps:
1) it prepares amino acid fermentation bacteria residue: amino acid fermentation bacteria residue is made in the waste water after amino acids production after primary filtration；
2) by amino acid fermentation bacteria residue made from step 1) as the ingredient of the fermentation medium in neomycin fermenting and producing, part
Substitution or replacing whole soybean powder are as fermentation raw material production neomycin；
Amino acid fermentation bacteria residue made from step 1) is detected, 10% amino acid is not less than using dry biomass ratio
Bacteria residue, total nitrogen analysis knot fruit≤45% in amino acid bacteria residue；
In step 2, the mass ratio that amino acid fermentation bacteria residue substitutes soybean powder is the 50-100% of soybean powder quality.
2. a kind of method using amino acid fermentation bacteria residue substitution fermentation raw material production neomycin according to claim 1,
It is characterized by: the waste water after amino acids production is carried out primary filtration using ceramic membrane in step 1).
3. a kind of method using amino acid fermentation bacteria residue substitution fermentation raw material production neomycin according to claim 1,
It is characterized in that each component parts by weight of fermentation medium are as follows in step 2:
750 soybean powder 0-225 amino acid fermentation bacteria residue 225-450 of rice flour
60 oral glucose of corn pulp, 210 amylase 3
75 calcium carbonate of ammonium sulfate, 67.5 sodium chloride 67.5
1.5 sodium dihydrogen phosphate of potassium dihydrogen phosphate, 7.5 silicone oil 15.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101126064A (en) * | 2007-07-18 | 2008-02-20 | 武汉烁森生态科技有限公司 | Biological modified method for erythromycin bacterium slag and reuse thereof |
| CN103461648A (en) * | 2013-09-23 | 2013-12-25 | 精晶药业股份有限公司 | Method for preparing feed by virtue of secondary fermentation of amino acid fermentation hyphae |
| CN103667398A (en) * | 2013-12-27 | 2014-03-26 | 宜昌三峡制药有限公司 | Method for producing neomycin by replacing part of fermenting raw materials with soybean meal |
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- 2015-12-24 CN CN201510991909.2A patent/CN105400852B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101126064A (en) * | 2007-07-18 | 2008-02-20 | 武汉烁森生态科技有限公司 | Biological modified method for erythromycin bacterium slag and reuse thereof |
| CN103461648A (en) * | 2013-09-23 | 2013-12-25 | 精晶药业股份有限公司 | Method for preparing feed by virtue of secondary fermentation of amino acid fermentation hyphae |
| CN103667398A (en) * | 2013-12-27 | 2014-03-26 | 宜昌三峡制药有限公司 | Method for producing neomycin by replacing part of fermenting raw materials with soybean meal |
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