CN105382881B - Puncher for countercurrent immunoelectrophoresis agar plate - Google Patents
Puncher for countercurrent immunoelectrophoresis agar plate Download PDFInfo
- Publication number
- CN105382881B CN105382881B CN201510957454.2A CN201510957454A CN105382881B CN 105382881 B CN105382881 B CN 105382881B CN 201510957454 A CN201510957454 A CN 201510957454A CN 105382881 B CN105382881 B CN 105382881B
- Authority
- CN
- China
- Prior art keywords
- button
- sheet frame
- fixing sheet
- fixed
- fixed plate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B26—HAND CUTTING TOOLS; CUTTING; SEVERING
- B26F—PERFORATING; PUNCHING; CUTTING-OUT; STAMPING-OUT; SEVERING BY MEANS OTHER THAN CUTTING
- B26F1/00—Perforating; Punching; Cutting-out; Stamping-out; Apparatus therefor
- B26F1/02—Perforating by punching, e.g. with relatively-reciprocating punch and bed
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B26—HAND CUTTING TOOLS; CUTTING; SEVERING
- B26D—CUTTING; DETAILS COMMON TO MACHINES FOR PERFORATING, PUNCHING, CUTTING-OUT, STAMPING-OUT OR SEVERING
- B26D7/00—Details of apparatus for cutting, cutting-out, stamping-out, punching, perforating, or severing by means other than cutting
- B26D7/18—Means for removing cut-out material or waste
- B26D7/1845—Means for removing cut-out material or waste by non mechanical means
- B26D7/1863—Means for removing cut-out material or waste by non mechanical means by suction
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B26—HAND CUTTING TOOLS; CUTTING; SEVERING
- B26D—CUTTING; DETAILS COMMON TO MACHINES FOR PERFORATING, PUNCHING, CUTTING-OUT, STAMPING-OUT OR SEVERING
- B26D7/00—Details of apparatus for cutting, cutting-out, stamping-out, punching, perforating, or severing by means other than cutting
- B26D7/26—Means for mounting or adjusting the cutting member; Means for adjusting the stroke of the cutting member
- B26D7/2628—Means for adjusting the position of the cutting member
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- Life Sciences & Earth Sciences (AREA)
- Forests & Forestry (AREA)
- Engineering & Computer Science (AREA)
- Mechanical Engineering (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a puncher for a countercurrent immunoelectrophoresis agar plate. The puncher comprises a punching tube, a fixing plate, a fixing plate frame, a handle and a negative-pressure barrel, wherein the punching tube is communicated with the negative-pressure barrel through a hose; the inner side of the opposite side of the fixing plate frame is provided with a slide path; the two ends of the fixing plate can be arranged in the slide path in a sliding mode; at least one end of the fixing plate is provided with a stirring block; the stirring block is positioned at the outer side of the fixing plate frame; a slide slot for allowing the stirring block to slide is formed in the fixing plate frame; a displacement sensor is arranged in the slide path; and a displacement display screen is arranged on the fixing plate frame or the handle. The puncher can be used for punching a plurality of holes in the agar agarose gel plate once and can automatically suck out agar in the holes, so that the whole operation can be completed by only one hand, and therefore, the puncher is efficient and convenient.
Description
Technical field
The present invention relates to a kind of testing equipment, specifically a kind of counter immunoelectrophoresiss agar plate card punch.
Background technology
In counter immunoelectrophoretic test operation, needs are put in the agarose gel plate for having solidified on punching template drawing
Punched with glass tubing, then the agarose in hole is chosen with pin again.There is problems with present drill process：
1st, as the quantity punched is more, once need to make a call to tens holes, it is time-consuming using glass tubing punching one by one
Arduously, even and if have template drawing, but still have many holes train off, affect test result of determination.
2nd, after the completion of punching, in addition it is also necessary to which, with pin choosing the agarose in hole one by one, workload also compares
Greatly, and the agarose that is easily destroyed when agarose is chosen on agarose gel plate.
The content of the invention
For the problems referred to above, the present invention provides one kind and can once beat many holes to agarose gel plate, and can automatically by
The counter immunoelectrophoresiss agar plate card punch that agarose in hole is suctioned out.
The present invention solves above-mentioned technical problem and is adopted the technical scheme that：A kind of counter immunoelectrophoresiss agar plate punching
Device, it is characterised in that it includes perforated pipe, fixed plate, fixing sheet frame, handle and negative pressure cylinder, the perforated pipe is fixed on described
In fixed plate, the fixed plate is arranged in the fixing sheet frame；The handle is fixed on above the fixing sheet frame, described negative
Pressure cylinder is fixed on the handle；
The negative pressure cylinder includes cylinder, piston rod, piston, the first spring and stopping means, and the stopping means include limit
Position seat, button and second spring；The limit base includes the through hole of horizontal blind hole and longitudinal direction, and the limit base is arranged on cylinder
Top, longitudinal through hole and cylinder it is coaxial；The button is located in horizontal blind hole, and the second spring is located at described pressing
The bottom of button, is provided with convex button on the button, is provided with the card coordinated with the convex button in the upper end of the piston rod
Groove；Button pass through hole is provided with the cylinder, and the button passes the button pass through hole outside cylinder；
The piston rod passes through longitudinal through hole, arranges piston in the lower end of piston rod, and first spring is nested with
On the piston rod, and the upper end of the first spring is fixed on limit base, the lower end of the first spring is fixed on piston rod bottom；Institute
State cylinder body bottom and be provided with multiple passages, the perforated pipe is connected with the passage by flexible pipe；
Slideway is provided with the inside of the fixing sheet frame relative edge, and the two ends of the fixed plate are slidably disposed on institute
State in slideway, one shifting block is set at least one end of fixed plate, the shifting block is located on the outside of fixing sheet frame, in the fixed plate
The chute slided for the shifting block is provided with frame；
The displacement transducer being connected with the fixed plate is provided with the slideway, in the fixing sheet frame or handle
It is provided with displacement display screen.
Further, the position of the button pass through hole is provided with a support, the support includes crossbeam, in the horizontal stroke
It is hinged with beam by button press bar, the front end for pressing button press bar is located at the front of the button, is provided with the on the handle
Two buttons, the end for pressing button press bar are connected by rope with the second button.
Preferably, the fixed plate has two, is disposed side by side in fixing sheet frame, in each fixed plate is provided with one
The distance between 18 perforated pipes of row, a diameter of 3mm of each perforated pipe, two perforated pipes are 4mm.
Preferably, the handle includes the vertical rod for being fixed on the fixing sheet frame two ends and the horizontal stroke for being connected to two rod ends
Bar, the negative pressure cylinder are fixed on the cross bar.
Further, the nut that the shifting block is included bolt and coordinated with bolt.
The invention has the beneficial effects as follows：
1st, it can once beat many holes on agarose gel plate, not only efficiency high, and it is neat to punch；Kong Houneng is beaten
The negative pressure produced using negative pressure cylinder is drawn onto the agarose in hole in perforated pipe automatically, and then operator with negative pressure cylinder will can beat
Agarose blowout in the pipe of hole, eliminates after punching the step of choose agarose with pin, save a large amount of labour forces and when
Between.
2nd, perforated pipe is fixed in fixed plate, and fixed plate can be slided in fixing sheet frame, and combine displacement transducer,
Show the distance between adjacent two rows perforated pipe on displacement display screen, adjust two row's perforated pipes between apart from when, have position
The displacement for moving display screen shows, realizes accurately spacing and adjust.
3rd, handle is set in fixing sheet frame, on the cross bar of handle, fixes negative pressure cylinder, make whole device light and handy, be easy to behaviour
Make；Support settings button depression bar being adopted on negative pressure cylinder, the second button being set on handle, the second button of pressing is capable of achieving button
The linkage of depression bar, it is only necessary to singlehanded to complete whole operation, it is very convenient.
Description of the drawings
In order to be illustrated more clearly that the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
Accompanying drawing to be used needed for having technology description is briefly described, it should be apparent that, drawings in the following description are the present invention
Part preferred embodiment, for those of ordinary skill in the art, on the premise of not paying creative work, can be with
Other accompanying drawings are obtained according to these accompanying drawings.
Fig. 1 is the overall structure diagram of the present invention；
Fig. 2 is the negative pressure cylinder superstructure schematic diagram of the present invention；
Fig. 3 is the present invention looks up structural representation of the present invention；
Fig. 4 is the negative pressure cylinder cut-away view of the present invention；
Fig. 5 is fixing sheet frame end view of the present invention with displacement transducer.
In figure, 1 perforated pipe, 2 fixed plates, 21 connecting rods, 3 fixing sheet frames, slideway 31,32 chutes, 4 handles, 41 second are pressed
Button, 5 negative pressure cylinders, 51 cylinders, 511 passages, 52 piston rods, 521 draw-in grooves, 53 pistons, 54 first springs, 55 limit bases, 56 are pressed
Button, 561 convex buttons, 57 second springs, 6 flexible pipes, 7 shifting blocks, 71 bolts, 72 nuts, 8 displacement transducers, 9 display screens, 10 supports, 11
Button press bar is pressed in crossbeam, 12.
Specific embodiment
1-5 elaborates to the specific embodiment of the present invention below in conjunction with the accompanying drawings：
As shown in figure 1, the present invention includes perforated pipe 1, fixed plate 2, fixing sheet frame 3, handle 4 and negative pressure cylinder 5.Perforated pipe 1
It is arranged in fixed plate 2, fixed plate is arranged in fixing sheet frame 3, handle is arranged in fixing sheet frame 3, negative pressure cylinder 5 and perforated pipe
1 connection, the negative pressure produced using negative pressure cylinder 5 can be drawn onto agarose in perforated pipe, it is also possible to automatically by agarose from punching
Blowout in pipe.
The perforated pipe 1 is fixed in the fixed plate 2, and the fixed plate 2 is arranged in the fixing sheet frame 3.This
Bright first embodiment is that fixed plate 2 is directly anchored in fixing sheet frame 3.
But, in order to realize the distance between two row's perforated pipes 1, devise the second embodiment of the present invention：By fixed plate 2
Arrange two, side by side and it is slidable be arranged in fixing sheet frame 3, be provided with the punching of a row 18 in each fixed plate 2
The distance between pipe 1, a diameter of 3mm of each perforated pipe 1, two perforated pipes 1 are 4mm.Specific implementation is：Described solid
Slideway 31 is provided with the inside of 3 relative edge of fixed board frame, and the two ends of the fixed plate 2 are slidably disposed in the slideway 31,
One shifting block 7 is set in one end of fixed plate 2, or two ends are provided with shifting block 7, the shifting block 7 is located at outside fixing sheet frame 3
Side, is provided with the fixing sheet frame 3 and is connected with slideway 31, for the chute 32 that the shifting block 7 slides.Preferably, described group
The nut 72 that block 7 is included bolt 71 and coordinated with bolt 71.The connecting rod 21 of one end of bolt 71 and one end of fixed plate 2 is fixed
Connection, the other end are coordinated with nut 72 after stretching out chute 32.As long as tightening nuts during use, can be by fixed plate 2 and fixed plate
Frame 3 is fixed together.In order to accurately move fixed plate 2, the accuracy of punching distance is realized, set in the slideway 31
The displacement transducer 8 being connected with the fixed plate 2 is equipped with, displacement transducer 8 can adopt capacitive displacement transducer.Described solid
Displacement display screen 9 is provided with fixed board frame 3 or handle 4, the display screen 9 in Fig. 1 has been arranged on the lower end of handle 4.For displacement
Measured value is shown sensor technology on a display screen, belongs to prior art, in setting using displacement sensor displacement
Generally adopt for upper, repeat no more here.
The handle 4 is fixed on above the fixing sheet frame 3, and the negative pressure cylinder 5 is fixed on the handle 4.Described
Handss 4 include the vertical rod for being fixed on 3 two ends of the fixing sheet frame and the cross bar for being connected to two rod ends, and the negative pressure cylinder 5 is fixed
On the cross bar, in order to hold pleasant convenience during handle, sponges cover is provided with the cross bar of handle.Certainly, handle
4 can also be using structures such as arcs.
As shown in figure 4, the negative pressure cylinder 5 includes cylinder 51, piston rod 52, piston 53, the first spring 54 and stopping means,
The stopping means include limit base 55, button 56 and second spring 57；The limit base 55 includes horizontal blind hole and longitudinal direction
Through hole, the limit base 55 is arranged on above cylinder 51, and longitudinal through hole and cylinder 51 are coaxial.The button 56 is located at
In horizontal blind hole, the second spring 57 is located at the bottom of the button 56, is provided with convex button 561 on the button 56,
The draw-in groove 521 coordinated with the convex button 561, as shown in Figure 4,521 piston of draw-in groove is provided with the upper end of the piston rod 52
Draw-in groove of the part for bar 52 in horn-like formation, certainly, draw-in groove can be designed directly to around the piston rod annular slot of a week.
Button pass through hole is provided with the cylinder 51, and the button 56 passes the button pass through hole outside cylinder 51.
The piston rod 52 passes through longitudinal through hole, arranges piston, first spring in the lower end of piston rod 52
54 are nested with piston rod 52, and the upper end of the first spring 54 is fixed on limit base 55, and the lower end of the first spring 54 is fixed on
52 bottom of piston rod.51 bottom of the cylinder is provided with multiple passages 511, the perforated pipe 1 is logical with described by flexible pipe 6
Pore 511 is connected.
In order to realize the piston 53 of singlehanded i.e. releasable negative pressure cylinder 5, the position of the button pass through hole is provided with one
Individual support 10, support 10 are included in the symmetrically arranged horizon bar in the button pass through hole left and right sides, are vertically arranged in horizon bar front end
Vertical bar, and the crossbeam 11 on two vertical bar tops of connection.It is hinged with the crossbeam 11 by button press bar 12, the button
The front end of depression bar 12 is located at the front of the button 56, is provided with the second button 41 on the handle 4, described by button press bar 12
End be connected by rope with the second button 41.During use, the thumb for holding the handss of handle presses the second button 41, and drive is pressed
12 action of button press bar, by the front end push button 56 of button press bar 12, by piston release.
During use, first piston rod 52 is pressed, the convex button 561 on button 56 is kept off in the draw-in groove 521 of piston rod 52.To beat
Hole pipe 1 is directed at the agarose gel plate for having solidified, and after pressure perforated pipe 1, press...with one's finger down the second button 41, by button press bar 12
Front end presses the button 56, and convex button 561 removes draw-in groove 521, and bar 53 living resets under the effect of the first spring 54, in cylinder forms negative
Pressure, the agarose in perforated pipe 1 is drawn onto in perforated pipe.After removing the equipment, lower piston rod 52 is pressed quickly, will using air-flow
Agarose blowout in perforated pipe.If necessary to adjust the distance between two row's perforated pipes 1, then nut 72 is unclamped i.e. slidably solid
Fixed board, according to the display of display screen 9, reaches tightening nuts 72 after correct position.
The above combines accompanying drawing and the preferred embodiment of the present invention and embodiment is described above in detail, but the present invention is not
The above-described embodiment and examples are confined to, for those skilled in the art, without departing from structure of the present invention
On the premise of think of, some improvement and modification can also be made, these improve and modification also should be regarded as protection scope of the present invention.
Claims (5)
1. a kind of counter immunoelectrophoresiss agar plate card punch, it is characterised in that it include perforated pipe, fixed plate, fixing sheet frame,
Handss and negative pressure cylinder, the perforated pipe are fixed in the fixed plate, and the fixed plate is arranged in the fixing sheet frame；Described
Handss are fixed on above the fixing sheet frame, and the negative pressure cylinder is fixed on the handle；
The negative pressure cylinder includes cylinder, piston rod, piston, the first spring and stopping means, the stopping means include limit base,
Button and second spring；The limit base includes the through hole of horizontal blind hole and longitudinal direction, and the limit base is arranged on above cylinder,
Longitudinal through hole and cylinder are coaxial；The button is located in horizontal blind hole, and the second spring is located at the button
Bottom, is provided with convex button on the button, is provided with the draw-in groove coordinated with the convex button in the upper end of the piston rod；Institute
State, the button passes the button pass through hole outside cylinder；
The piston rod passes through longitudinal through hole, arranges piston in the lower end of piston rod, and first spring is nested with is living
On stopper rod, and the upper end of the first spring is fixed on limit base, and the lower end of the first spring is fixed on piston rod bottom；In the cylinder
Body bottom is provided with multiple passages, and the perforated pipe is connected with the passage by flexible pipe；
Slideway is provided with the inside of the fixing sheet frame relative edge, and the two ends of the fixed plate are slidably disposed on the cunning
In road, one shifting block is set at least one end of fixed plate, the shifting block is located on the outside of fixing sheet frame, in the fixing sheet frame
It is provided with the chute slided for the shifting block；
The displacement transducer being connected with the fixed plate is provided with the slideway, is arranged in the fixing sheet frame or handle
There is displacement display screen.
2. counter immunoelectrophoresiss agar plate card punch according to claim 1, it is characterised in that in the button pass through hole
Position is provided with a support, and the support includes crossbeam, is hinged with by button press bar on the crossbeam, described by button press bar
Front end is located at the front of the button, is provided with the second button on the handle, and the end by button press bar is pressed with second
Button is connected by rope.
3. counter immunoelectrophoresiss agar plate card punch according to claim 1, it is characterised in that the fixed plate has two
Bar, is disposed side by side in fixing sheet frame, is provided with 18 perforated pipes of a row, the diameter of each perforated pipe in each fixed plate
It is 4mm for the distance between 3mm, two perforated pipes.
4. counter immunoelectrophoresiss agar plate card punch according to claim 1, it is characterised in that the handle includes fixing
The fixing sheet frame two ends vertical rod and be connected to the cross bar of two rod ends, the negative pressure cylinder is fixed on the cross bar.
5. counter immunoelectrophoresiss agar plate card punch according to claim 1, it is characterised in that the shifting block includes bolt
And the nut coordinated with bolt.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510957454.2A CN105382881B (en) | 2015-12-18 | 2015-12-18 | Puncher for countercurrent immunoelectrophoresis agar plate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510957454.2A CN105382881B (en) | 2015-12-18 | 2015-12-18 | Puncher for countercurrent immunoelectrophoresis agar plate |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105382881A CN105382881A (en) | 2016-03-09 |
| CN105382881B true CN105382881B (en) | 2017-03-22 |
Family
ID=55415984
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510957454.2A Expired - Fee Related CN105382881B (en) | 2015-12-18 | 2015-12-18 | Puncher for countercurrent immunoelectrophoresis agar plate |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105382881B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106881747A (en) * | 2017-04-05 | 2017-06-23 | 巩红霞 | A kind of agar gel negative pressure forming hole method and perforating device |
| CN109203080A (en) * | 2018-09-27 | 2019-01-15 | 佛山科学技术学院 | A kind of agar perforating device |
| CN111660365B (en) * | 2020-05-28 | 2022-02-22 | 许子涵 | Agarose gel plate punching device and control method thereof |
| CN111660366B (en) * | 2020-05-28 | 2023-08-15 | 许子涵 | Agarose gel plate punching structure |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3708055A (en) * | 1971-06-28 | 1973-01-02 | Kraftco Corp | Dispensing method and apparatus |
| CN201251580Y (en) * | 2008-08-29 | 2009-06-03 | 河北科技大学 | Punching external member box of agar plate |
| CN102848413B (en) * | 2012-09-20 | 2015-09-09 | 秦皇岛瑞晶太阳能科技有限公司 | SLD cuts laydown machine automatically |
| CN203019467U (en) * | 2012-11-27 | 2013-06-26 | 承美机械有限公司 | Buffering structure of vacuum forming cutting machine |
| CN204209742U (en) * | 2014-10-24 | 2015-03-18 | 浙江洁美电子科技股份有限公司 | The device of dust on a kind of automatic removing carrier band cutting tool |
| CN204604382U (en) * | 2015-05-15 | 2015-09-02 | 四川农业大学 | Adjustable gel cutting cutter |
| CN205255138U (en) * | 2015-12-18 | 2016-05-25 | 青岛农业大学 | Counter immunoelectrophoresis agar plate hole puncher |
-
2015
- 2015-12-18 CN CN201510957454.2A patent/CN105382881B/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN105382881A (en) | 2016-03-09 |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170322 Termination date: 20201218 |
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| CF01 | Termination of patent right due to non-payment of annual fee |