CN105378062A - 包含分离的肾细胞的类器官及其用途 - Google Patents
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- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/22—Urine; Urinary tract, e.g. kidney or bladder; Intraglomerular mesangial cells; Renal mesenchymal cells; Adrenal gland
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/44—Vessels; Vascular smooth muscle cells; Endothelial cells; Endothelial progenitor cells
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P13/00—Drugs for disorders of the urinary system
- A61P13/12—Drugs for disorders of the urinary system of the kidneys
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0684—Cells of the urinary tract or kidneys
- C12N5/0686—Kidney cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/25—Urinary tract cells, renal cells
- C12N2502/256—Renal cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/28—Vascular endothelial cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2513/00—3D culture
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/50—Proteins
- C12N2533/54—Collagen; Gelatin
Abstract
Description
标记物 | 隔室 | ZSF1p0%阳性 |
AQP2 | 集合管 | 42.36 |
DBA | 远端肾小管 | 56.93 |
LTA | 近端的/环/IMCD | 39.62 |
CK18 | 泛上皮 | 68.39 |
量度 | 正常的 | 未处理的 | NKO处理的 |
肾小管-间质损伤 | 0.0+0.1 | 1.4±0.4 | 1.8±0.4 |
肾小球损伤 | 0.0 | 0.8±0.3 | 1.2±0.5 |
Claims (52)
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
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US201361855146P | 2013-05-08 | 2013-05-08 | |
US61/855,146 | 2013-05-08 | ||
US201361855152P | 2013-05-09 | 2013-05-09 | |
US61/855,152 | 2013-05-09 | ||
PCT/US2014/037275 WO2014182885A2 (en) | 2013-05-08 | 2014-05-08 | Organoids comprising isolated renal cells and uses thereof |
Publications (1)
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CN105378062A true CN105378062A (zh) | 2016-03-02 |
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US (2) | US20160101133A1 (zh) |
EP (2) | EP2994528B1 (zh) |
JP (3) | JP6490669B2 (zh) |
KR (6) | KR102556951B1 (zh) |
CN (1) | CN105378062A (zh) |
AU (3) | AU2014262670B2 (zh) |
BR (1) | BR112015027996A8 (zh) |
CA (1) | CA2911291A1 (zh) |
DK (1) | DK2994528T3 (zh) |
FI (1) | FI2994528T3 (zh) |
HR (1) | HRP20240170T1 (zh) |
PL (1) | PL2994528T3 (zh) |
PT (1) | PT2994528T (zh) |
RU (1) | RU2722361C2 (zh) |
SI (1) | SI2994528T1 (zh) |
WO (1) | WO2014182885A2 (zh) |
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CN109415680A (zh) * | 2016-05-18 | 2019-03-01 | 学校法人庆应义塾 | 类器官培养用细胞培养基、培养方法及类器官 |
CN112912068A (zh) * | 2018-05-10 | 2021-06-04 | 伊井正明 | 包封有抑制素的纳米颗粒制剂、含有其的牙髓源性干细胞和含有该干细胞的细胞制剂 |
CN113056277A (zh) * | 2018-08-31 | 2021-06-29 | 蒂莫西.伯特伦 | 包含细胞衍生的囊泡的组合物及其用途 |
CN114732832A (zh) * | 2022-04-08 | 2022-07-12 | 宁夏医科大学总医院 | 一种防***的可植入物 |
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WO2011140441A2 (en) | 2010-05-06 | 2011-11-10 | Children's Hospital Medical Center | Methods and systems for converting precursor cells into intestinal tissues through directed differentiation |
AU2013334276B2 (en) | 2012-10-24 | 2019-07-04 | Prokidney | Renal cell populations and uses thereof |
KR102181336B1 (ko) | 2013-06-13 | 2020-11-24 | 오르제네시스 엘티디. | 세포 집단, 이의 전환분화 방법 및 이용 방법 |
AU2015267148B2 (en) | 2014-05-28 | 2021-07-29 | Children's Hospital Medical Center | Methods and systems for converting precursor cells into gastric tissues through directed differentiation |
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CA2969592C (en) | 2014-12-05 | 2023-02-14 | University Of Florida Research Foundation, Inc. | 3d printing using phase changing materials as support |
MA41296A (fr) | 2014-12-30 | 2017-11-07 | Orgenesis Ltd | Procédés de transdifférenciation et procédés d'utilisation de ceux-ci |
US11007705B2 (en) | 2015-02-13 | 2021-05-18 | University Of Florida Research Foundation, Inc. | High speed 3D printing system for wound and tissue replacement |
WO2016182969A1 (en) | 2015-05-08 | 2016-11-17 | University Of Florida Research Foundation, Inc. | Growth media for three-dimensional cell culture |
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WO2017041041A1 (en) * | 2015-09-03 | 2017-03-09 | The Brigham And Women's Hospital, Inc. | Three-dimensional differentiation of epiblast spheroids to kidney organoids models stage-specific epithelial physiology, morphogenesis, and disease |
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US10767164B2 (en) | 2017-03-30 | 2020-09-08 | The Research Foundation For The State University Of New York | Microenvironments for self-assembly of islet organoids from stem cells differentiation |
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WO2019006127A1 (en) * | 2017-06-28 | 2019-01-03 | Rutgers, The State University Of New Jersey | ORGANOIDS DERIVED FROM A SINGLE RENAL CELL |
KR102094929B1 (ko) * | 2018-07-06 | 2020-03-30 | 한국생명공학연구원 | 신장 스페로이드 및 그의 제조 방법 |
KR102118807B1 (ko) | 2018-11-26 | 2020-06-03 | 숭실대학교산학협력단 | 3차원 오가노이드 제조용 형상변화형 하이드로젤 몰드 조성물 및 이를 통해 제조된 몰드 |
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Publication number | Priority date | Publication date | Assignee | Title |
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CN109415680A (zh) * | 2016-05-18 | 2019-03-01 | 学校法人庆应义塾 | 类器官培养用细胞培养基、培养方法及类器官 |
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