CN105377280A - Medicine for blood enrichment and preparation method therefor - Google Patents

Medicine for blood enrichment and preparation method therefor Download PDF

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CN105377280A
CN105377280A CN201380078231.8A CN201380078231A CN105377280A CN 105377280 A CN105377280 A CN 105377280A CN 201380078231 A CN201380078231 A CN 201380078231A CN 105377280 A CN105377280 A CN 105377280A
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albiflorin
paeoniflorin
medicament
enriching blood
thing
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CN105377280B (en
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张建军
李伟
王景霞
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Beijing University of Chinese Medicine
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Beijing University of Chinese Medicine
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/65—Paeoniaceae (Peony family), e.g. Chinese peony
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/06—Antianaemics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine

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Abstract

A use of albiflorin in the preparation of blood-enriching medicines, and a preparation method of albiflorin are provided. Albiflorin is prepared according to the following method: extracting radix paeoniae alba crude drug with water or alcohol as solvent; refining the radix paeoniae alba extracting solution with macroporous resin; refining the macroporous resin refined substance with alumina; refining the alumina refined solution with activated carbon; refining the activated carbon refined substance with silica gel; adding an eluent, which is 8-20 times the volume of the activated carbon refined substance, to the activated carbon refined substance for dissolution, eluting with the eluent through a silica gel column, collecting fractions, performing thin-layer inspection, collecting the fractions containing paeoniflorin, the fractions containing paeoniflorin and albiflorin, and the fractions containing albiflorin respectively, recovering all the solvents, dissolving the extract in water, filtering, and drying the filtrate to obtain paeoniflorin, the mixture of paeoniflorin and albiflorin, and albiflorin, wherein the content of albiflorin is not less than 92%.

Description

Medicine for blood enrichment and preparation method therefor
It is a kind of for medicine for enriching blood and preparation method thereof
Technical field
The present invention relates to medicine of a kind of blood tonification effect and preparation method thereof, and in particular to albiflorin of blood tonification effect and preparation method thereof is played in the root of herbaceous peony, belongs to pharmaceutical technology field.
Background technology
The root of herbaceous peony is ranunculaceae plant Chinese herbaceous peony i Paeonia lacti flora Pal l.) dry root, be that the traditional Chinese medical science often uses antianaemics, bitter, acid, it is slightly cold, Return liver, the spleen channel, with nourishing blood for regulating menstruation, astringing YIN to stop sweating, soft liver analgesic, effect of suppressing liver-YANG, for blood deficiency chlorosis, irregular menstruation, spontaneous perspiration, night sweat, hypochondriac pain, stomachache, four limbs contraction pain, dizziness of having a headache.
The root of herbaceous peony contains one group of Monoterpenes, including Paeoniflorin, albiflorin etc..Also, Paeoniflorin has clear and definite blood tonification effect.Because albiflorin is difficult to isolate and purify, albiflorin is rarely reported in patent and document.It is therefore desirable to albiflorin single component is studied.
The content of the invention
It is a kind of for medicine for enriching blood and preparation method thereof present invention aims at providing, albiflorin of a kind of blood tonification effect and preparation method thereof is specifically provided.
Application of the albiflorin in medicament for enriching blood is prepared.
Application of the albiflorin in the medicine for preparing treatment Blood deficiency.
A kind of albiflorin for enriching blood, the medicine is made by the following method：
Step 1:Extract：White Peony Root is extracted using water or alcohol as solvent through conventional method, and extract solution obtains white paeony root extract standby through dense Shrink, filtering；
Step 2:Macroreticular resin is refined：White paeony root extract, by macroporous resin column, discards efflux；Resin column is rinsed with 0 20% alcoholic solvent, flushing liquor is discarded；Resin column is eluted with 30 70% alcoholic solvents, eluent is collected, alcohol is reclaimed through Jian Ya Nong Shrink, is dried under reduced pressure, macroreticular resin is obtained and refines thing.
Step 3:Aluminum oxide is refined：The smart thing of macroreticular resin is taken to add water or alcohol dissolving, root of herbaceous peony macroreticular resin refines thing and adds the aluminum oxide of 2-8 times of weight to mix, dries, and loads percolator, with eluent, collects whole effluxes, obtains aluminum oxide refined liquid standby；
Step 4:Absorbent charcoal fine purification：Aluminum oxide refined liquid collects efflux by activated-charcoal column, reclaims whole solvents, dries, obtain absorbent charcoal fine purification thing standby；
Step 5:Silica gel is refined：8 20 volume eluant, eluents are added to dissolve absorbent charcoal fine purification thing, pass through silicagel column, with eluent, collect stream part, thin layer is inspected, the stream part containing Paeoniflorin, Paeoniflorin and albiflorin, albiflorin is collected respectively, reclaims whole solvents, Paeoniflorin, Paeoniflorin and albiflorin mixture, albiflorin are obtained respectively.
Step 6:Paeoniflorin and the albiflorin mixture presses the method for above-mentioned steps 5, repeats refined, obtains Paeoniflorin, albiflorin.
It is preferred that, in step 1, root of herbaceous peony Extraction solvent is water, and extracting method is diacolation, 14 times of medicinal material amount/hours of flow velocity, collects the percolate of 6 15 times of root of herbaceous peony times of weight； It is preferred that, in step 2, the macroreticular resin is one kind in ME-1, D- 101, DA- 201, D- 301, AB-8；It is preferred that, in step 2, the alcoholic solution is the aqueous solution of methanol, ethanol or propyl alcohol.
It is preferred that, in step 2, macroreticular resin consumption is root of herbaceous peony 1-3 times of weight.Flow velocity is 0.5 3 times of amount of resin/hours.0 20% alcoholic solvent of the resin column with 15 macroreticular resin times of weight is rinsed, flushing liquor is discarded.The eluent of collection is 30 70% alcoholic solvents of 38 macroreticular resin times of weight；
It is preferred that, in step 3, root of herbaceous peony macroreticular resin refines thing and adds the aluminum oxide of 4 times of weight to mix；Collect the 4-8 times of weight that eluent is aluminum oxide；
It is preferred that, in step 4, activated-charcoal column refines thing 1-6 times of weight, preferably 2 times of weight equivalent to root of herbaceous peony macroreticular resin；The absorbent charcoal fine purification thing of preparation is more than 95% containing Paeoniflorin and albiflorin sum.
Eluant, eluent used in above-mentioned steps 3 and step 5 can be made up of following one or more solvents mixing, and ratio can be according to equivalent to ethyl acetate：Methanol is 3-9:1 polarity is selected or mixed：
Liquid alcohols：Methanol, ethanol, propyl alcohol, propyl alcohol etc.；
Liquid esters：Methyl formate, Ethyl formate, methyl acetate, ethyl acetate, propyl acetate, butyl acetate, pentyl acetate etc.；
Liquid ketone：Acetone, butanone, pentanone etc.；
Liquid alkane class：Petroleum ether, pentane, hexane, heptane, octane etc.；
Liquid ethers：Ether, propyl ether, butyl, methyl tertiary butyl ether(MTBE) etc.；
Liquified Halon class：Chloroform, dichloromethane, carbon tetrachloride etc.；
For example：Pentanone；Butyl acetate-ethanol 8:1;Ethyl formate-ethanol 6:1;Butanone-isopropanol 7:1;Dichloromethane-acetone 3:1;Methylene chloride-methanol 5:1;Butanone-propyl alcohol 9:2;Methyl tertiary butyl ether(MTBE)-butanone 5:2 etc..
It is preferred that, it is that above-mentioned absorbent charcoal fine purification thing is 0.02-0.09g/cm than silicagel column cross-sectional area than applied sample amount in step 52, flow velocity is 1-4 times of silica gel amount/hour,
The thin layer inspection method of above-mentioned steps 5 can be the contained method of prior art, be preferably as follows method：
Take silicagel column respectively to elute stream part respectively, be used as need testing solution.Separately take Paeoniflorin, albiflorin reference substance, plus ethanol that every 1ml respectively solution containing lmg is made, be used as reference substance solution.According to thin-layered chromatography experiment, the above-mentioned μ 1 of need testing solution 20, standard solution Ι θ μ are drawn, is put respectively on same silica GF254 lamellae, with 4:1 acetate-methanol is solvent, is deployed, and takes out, dries, put and inspected under ultraviolet lamp (254nm).Inspected with thin layer, it is that ^ there are the vertical stream part Households tires of single interior tenth of the twelve Earthly Branches ^3 ^ to be to take aim at the interior tenth of the twelve Earthly Branches to have contained by single stream part；Two vertical stream part Households tires of standing grain, Nei You Lose be ^ W standing grain in tenth of the twelve Earthly Branches Beach not；Close respectively chaste tree stream part § Ah.
The corresponding relation of volume and weight of the present invention be ml withgRelation.
A kind of pharmaceutical preparation for enriching blood, amount of the per unit preparation containing albiflorin is that 90mg 270mg/ days derive calculating according to daily preparation total amount.The unit formulation refers to pharmaceutically conventional preparation unit, and such as capsule is every, and granule is every bag, and oral liquid is every milliliter etc.. It is preferred that, amount of the per unit preparation containing albiflorin is 120mg or 180mg or 240mg according to daily preparation total amount.Albiflorin of the present invention is used for medicament for enriching blood and preparation method thereof, with advantages below：
1. purify：The big carbohydrate of depolarization and Hydroxy peoniflorin, benzoylpaeoniflorin, tannin, organic acid, flavones, triterpene glycoside impurity with phenolic hydroxyl group and carboxyl are removed with alumina column respectively, the small pigment impurity of depolarization is removed with activated-charcoal column；Obtained albiflorin, Paeoniflorin mixture purity 95%, it is to avoid silicagel column may be reused on silica gel in impurity absorption, simplicity operation, can continuously be produced.
2. specific sample loading mode：Albiflorin, Paeoniflorin mixture are dissolved in eluant, eluent and add silicagel column, without drying process, silica gel is weaker to albiflorin, Paeoniflorin adsorption capacity, although the product purity of preparation is relatively low, but content still ＞ 90%, meets the standard of a kind new medicine bulk drug；And elution rate is high, production hour is few（Each loading is eluted about 2 hours）；Because silicagel column may be reused, only first time loading when there is part albiflorin, Paeoniflorin to elute, can all elute later, product yield is high.
3. the mixed solvent eluant, eluent of fixed proportion：Silicagel column is using the ratio of fixed mixed solvent as elution, and recovered solvent may be reused, and reduce cost；
4. industrial production is feasible：Because silicagel column is reusable, without dismounting, and loading uses solution loading, can pipeline, can realize production equipment totally-enclosed, control because it is inflammable, explosive caused by organic solvent volatilization, poison, the harm such as pollution.
5. the product being made, paeoniflorin content 95%, albiflorin content 92% meets a kind new medicine bulk drug standard.
6. albiflorin has blood tonification effect, and the blood tonification effect of albiflorin is better than Paeoniflorin.
7. the effective dose of albiflorin blood tonification effect is low, and albiflorin has stronger blood tonification effect.Experimental example 1
1 experiment material
1. 1 experimental animal
Male mice in kunming, 22 ± 2g of body weight.Animal ties up the magnificent experimental animal Co., Ltd of tonneau by Beijing and provided, licensing numbering SCXK (capital） 2012-0001.
1. 2 medicines
By reagent：Paeoniflorin, albiflorin.
It is prepared by Paeoniflorin, albiflorin（Prepared by embodiment 2）：
Determined through HPLC methods, paeoniflorin content is 98. 1%, albiflorin content is 93. 2%.
Positive drug：Siwu Tang particle, lucky Tai'an（Sichuan）Pharmaceutcal corporation, Ltd, lot number： 1206064.Determined through HPLC methods, paeoniflorin content is 0. 536%.
1. 3 instruments and reagent
The cellanalyzers of Beckman Coulter Ac. T 5, U.S. Beckman Coulter Inc.Beckman Coulter Ac. T 5diff Rinse, U.S. Beckman Coulter Inc, lot number： 03202C21926;Ac. T 5diff WBC Lyse, U.S. Beckman Coulter Inc, lot number： 16502B02838;Ac. T 5diff Fix, U.S. Beckman Coulter Inc, lot number： 17602E07007;Ac. T 5diff Hgb Lyse, U.S.'s Beckman Coulter Inc lot numbers： 04802A0170o
Experimental method
2.1 Paeoniflorins and the experiment of albiflorin group
After adaptability is fed, 90 mouse are randomly divided into 9 groups, i.e. normal group, model of blood dificiency group, Siwu Tang particle group, the dosage group of Paeoniflorin 123, albiflorin 12,3 dosage groups, every group 10 by body weight.The wherein daily gavage distilled water of Normal group, model group, the dosage group of Paeoniflorin 123 give respectively the mg kg of Paeoniflorin 120-1, 60 mg · kg—1, 30 mg · kg—1, albiflorin 12,3 dosage groups give respectively albiflorin 120mg kg-1, 60 mg · kg—1, 30 g-be administered by every mL of 10 g body weight gavage 0.2, once a day.
Mouse successive administration 7 days, then carries out the modeling of two weeks by a definite date.Model group, Siwu Tang particle group, Paeoniflorin dosage group, peony lactone dosage group every other day by eyeground vein clump the mL of bloodletting 0.3, next day warm water swim 20 min, while daily watch material by 75 g-kg-1Administration is continued to during body weight control, free water, modeling.In eye socket blood sampling 40 in the 0th 7 14 day, blood picture is detected.
2.2 statistical method
Experimental data carries out one-way AN0VA statistical analysis using SPSS softwares, is as a result represented with ± s.
3 experimental results
3.1.1 the influence of Paeoniflorin, albiflorin to red blood cell number
Modeling the 7th day, compared with blank group, the red blood cell number of model group is substantially reduced（P〈0.001).Compared with model group, positive drug group be Siwu Tang particle group, 120mg * kg-1Albiflorin, 120mg * kg-1The red blood cell number rise of Paeoniflorin, difference highly significant（P〈0.01)； 60mg · kg—1Albiflorin, 30mg kg-1Albiflorin red blood cell is raised, significant difference（P〈0.05).With Paeoniflorin compared with dosage group, 30mg kg-1The red blood cell number rise of albiflorin is obvious（P〈0.05).
Modeling the 14th day, compared with blank group, the red blood cell number of model group is substantially reduced（P〈0.001).Compared with model group, positive drug group be Siwu Tang particle group, 120mg * kg-1The red blood cell number rise of albiflorin, difference is extremely notable（P ＜ 0.001),
60mg * kg— 1Albiflorin, 30mg * kg-1The red blood cell number of albiflorin has the difference (Ρ ＜ 001) of highly significant,
120mg · kg—1Also there were significant differences for Paeoniflorin red blood cell (Ρ ＜ 0 05).With Paeoniflorin compared with dosage group, 120mg kg-1Albiflorin, 30mg * kg-1The red blood cell number rise of albiflorin is obvious（P〈0.05).It the results are shown in Table 1
The Paeoniflorin of table 1., influence of the albiflorin to mouse red blood cell
Dosage the 0th day the 7th day the 14th day
(mg-kg-1) (1012.L— ') (1012.L— ') (1012.L— ')
The Siwu Tang particle 2.5g-kg of 8 7.99+0.82 9.35 ± 0.63***, 8.42 ± 0.38*** models of blank 8 8.50 1.26 7.02 ± 0.43 6.09 ± 0.5918 8.88+1.48,8.27+0.56** 7.55 ± 0.96*** albiflorin 1 120 8 8.92 0.75 8.18 ± 0.45**, 7.39 ± 0.56***# albiflorins 2 60 8,7.93 ± 0.72* of 9.62+0.61,7.10 ± 0.62**
The 9.22+0.68 7.50 ± 0.60 6.51 ± 0.55 of the 6.99 ± 0.54**# of ± 1.29 7.89 ± 0.84*# of albiflorin 3 30 8 8.45 Paeoniflorins, 1 120 8 9.03 6.71 ± 0.52* of ± 0.88 7.90 ± 0.39** Paeoniflorins 2 60 8
Paeoniflorin 3 30 8 8.98 1.35 7.29 ± 0.58 6.26 ± 0.67
Note：With model group than the * * P of * P ＜ 0.05<0.01 ***P<0.001, albiflorin is with Paeoniflorin with dosage group than the ##P of #P ＜ 0.05<0.01 leakage P ＜ 0.001 3.1.2 the influence of Paeoniflorin, albiflorin to content of hemoglobin
Modeling the 7th day, compared with blank group, the content of hemoglobin of model group is substantially reduced（P〈0.001).Compared with model group, positive drug group be Siwu Tang particle group, 120mg * kg-1Albiflorin, 60mg * kg-1The content of hemoglobin rise of albiflorin is obvious, difference highly significant（P〈0.01); 30mg * kg—1Albiflorin, 120mg * kg-1The hemoglobin difference rise of Paeoniflorin is obvious, significant difference（P〈0.05).With Paeoniflorin compared with dosage group, the content of hemoglobin of albiflorin is without significant difference（P〉0.05).
Modeling the 14th day, compared with blank group, the content of hemoglobin of model group is substantially reduced（P〈0.001).Compared with model group, positive drug group be Siwu Tang particle group, 120mg * kg-1The content of hemoglobin rise difference of albiflorin is extremely notable
(Ρ〈0· 001),60mg · kg— 1Albiflorin, 30mg kg-1The hemoglobin difference highly significant (P ＜ 0.01) of albiflorin,
120mg * kg— 1Paeoniflorin content of hemoglobin significant difference (P ＜ 0.05);With Paeoniflorin same dose group ratio, 30mg kg-1The hemoglobin significant difference of albiflorin group（P〈0.05).It the results are shown in Table 2.
The Paeoniflorin of table 2., influence of the albiflorin to mouse hemoglobin
Group ,-N the 0th day（G/L) the 7th day（G/L) the 14th day（G/L) the # of the 119.00 ± 11.50*** of ± 15.86 127 ± 10.85*** of blank 8 112.5 models, 8 123.5 ± 13.75 105.00 ± 9.01 88.89 ± 10.54 Siwu Tang particle, 8 127.78 111.10 ± 14.74*** of ± 17.70 122.20 ± 8.33** albiflorins, 1 120 8 130.5 108.75 ± 6.41*** of ± 9.56 121.87 ± 10.67** albiflorins, 2 60 8 140.5 106.88 ± 9.32** of ± 9.84 118.13 ± 9.98** albiflorins, 3 30 8 125.56 ± 17.04 117.50 ± 6.56* 105.63 ± 9.43
101.25 ± 8.35* of ± 11.49 116.25 ± the 14.08* of Paeoniflorin 1 120 8 132.22
Paeoniflorin 2 60 8 135.0 ± 10.8 112.22 ± 12.02 98.75 ± 9.16
Paeoniflorin 3 30 8 131.11 ± 21.03 111.88 ± 8.43 92.22 ± 10.64
Note：With model group than * P ＜ 0.05, * * P<0.01, * * * P<0.001, albiflorin is with Paeoniflorin with dosage group than #P ＜ 0.05, ##P<0.01, leakage P ＜ 0.001
This experiment employs compound bleeding method model of blood dificiency, as a result display model group mouse peripheral blood as middle red blood cell and the reduction of hemoglobin number it is obvious, illustrate model establishment.Modeling the 7th day and the 14th day, positive drug group is Siwu Tang particle group, each dosage group red blood cell number of albiflorin and content of hemoglobin rise, that is the effective dose of albiflorin Paeoniflorin only has high dose group for 30mg kg-red blood cell number and content of hemoglobin is raised, i.e. the effective dose of Paeoniflorin is that 120mg kg-^ is contrasted with dosage group, modeling the 7th day, 30mg * kg-1The red blood cell number of albiflorin is higher than Paeoniflorin（P〈0.05);14th day,
120mg · kg— 1The red blood cell number of albiflorin is higher than Paeoniflorin（P ＜ 0.05), 30mg kg-1The red blood cell number of albiflorin, content of hemoglobin are all higher than Paeoniflorin（P〈0.05).Therefore, albiflorin is same with Paeoniflorin has blood tonification effect, and the blood tonification effect of albiflorin is better than Paeoniflorin.
For blood tonification effect of the research albiflorin to other model of blood dificiency, we cause mouse model of blood dificiency using radiation method, detect murine interleukin number, inquire into the blood tonification effect of albiflorin, see experimental example 2.
Experimental example 2
1 experiment material
1.1 Experimental Animals Male kunming mices 60,20 ± 2g of body weight.By Beijing, the magnificent animal experimental center of dimension tonneau is provided.The animal quality certification is numbered：SCXK (capital） 2012-0001. 1. 2 test medicine albiflorins
It is prepared by albiflorin（Prepared by embodiment 2）：Determined through HPLC methods, albiflorin content is 93. 2%.Positive drug SIWU KELI, lucky Tai'an（Sichuan）Pharmaceutcal corporation, Ltd, product batch number： 1206064.
1. 3 instruments and reagent Beckman Coulter Ac. T5 cellanalyzers, U.S. Beckman Coulter Inc.Beckman Coulter Ac. T 5diff Rinse, U.S. Beckman Coulter Inc, lot number： 03202C21926;Ac. T 5diff WBC Lyse, U.S. Beckman Coulter Inc, lot number： 16502B02838;Ac. T 5diff Fix, U.S. Beckman Coulter Inc, lot number： 17602E07007;Ac. T 5diff Hgb Lyse, U.S. Beckman Coulter Inc, lot number： 04802A0170.Y ray irradiations source is provided by Academy of Military Sciences's radiation.
Experimental method
2. 1 packet and administration
60 mouse are randomly divided into 6 groups, i.e. normal group, model of blood dificiency group, SIWU KELI group, 3 dosage groups of albiflorin, every group 10.It is administered by every 2ml of 10g body weight gavage 0., once a day.The daily gavage of Normal group, model group gives distilled water, albiflorin each group give respectively 120mg* kg -60mg- kg -30mg- kg -1Albiflorin.2. 2 model preparation methods
Animal adaptability is fed 5 days, the prevention administration before modeling, successive administration 7 days, is used within the 8th day6°C o gamma rays whole body irradiation 1 time, the 5Gy of irradiation dose 3., the Gy min of close rate 1. 60-1, make deficiency of blood model of a syndrome.Mouse gavage, continuous gavage 14d immediately after irradiation.Radiation day is calculated the 0th day, the 3rd after modeling, 7,10,14 days, and eye socket takes the μ 1 of blood 50, detects peripheral hemogram leukocyte count.
2. the processing of 3 statistical results
One-way AN0VA statistical analysis is carried out using SPSS softwares, as a result represented with x ± s.
3 results
Influence of the albiflorin to leukocyte count
Murine interleukin number substantially has decline after modeling, and model group leucocyte difference compared with blank group is extremely notable (P ＜ 0. 001);The leukocyte count of each irradiation group is also decreased significantly compared with blank group, illustrates modeling success；Modeling the 7th day, SIWU KELI group, 120mg- kg-1The leukocyte count of albiflorin is raised substantially compared with model group, significant difference（05), modeling the 10th day and the 14th day, the leucocyte of albiflorin each group has rise trend to P ＜ 0., and no significant difference (P ＞ 0. 05) the results are shown in Table 3. The albiflorin of table 5 causes the influence of deficiency of blood murine interleukin number to radiation technique
14 days 10 days group mg* kg of dosage the 3rd day 7 day-1 N (109 - L 1) (109 · L 1) (109 · L- (109The SIWU KELI 2.5g- kg of L- blank 8 8.72 ± 1.19***, 7.48 ± 0.72***, 9.20 ± 0.74***, 7.91 ± 1.18*** models 8 1.16 ± 0.23 1.65 ± 0.33 3.00 ± 0.57 3.80 ± 0.71-1± 0.17 2.20 ± the 0.60* 3.35 ± 0.67 4.00 ± 1.07 of 8 1.44 3.60 ± 0.43* of ± 0.93 2.35 ± 0.48*, 4.50 ± 0.56 albiflorins 120 8 1.12
60 8 1.12±0.32 2.09±0.39 3.05±0.37 3.80±0.77
30 8 1.12±0.25 2.10±0.35 3.14±0.63 3.90±0.67
* with model group than P ＜ 0.05, * is with model group than P ＜ 0.01, * * cause mouse model of blood dificiency than 0.001 experiment of P ＜ with model group using radiation method, find that murine interleukin number is significantly reduced after modeling, illustrate that model is set up.Give after albiflorin treatment, in modeling the 7th day, compared with model group, 120 mg* kg-1Leukocyte count rise is obvious, and difference has a statistical significance (P ＜ 0.05), modeling the 10th day and the 14th day, and the leucocyte of albiflorin each group also has rise trend.As a result show, albiflorin has the leukocyte count of rise radiation cause deficiency of blood mouse, with blood tonification effect.Brief description of the drawings
In order that present disclosure is more likely to be clearly understood, below according to specific embodiment of the invention and with reference to accompanying drawing, the present invention is further detailed explanation, wherein：
Fig. 1 is present invention process schematic flow sheet.
Embodiment
Embodiment 1:
Root of herbaceous peony 5kg is taken, is soaked 2 hours, decocts and extracts 2 times, 1 hour every time, add water 12 times, add water for the second time 10 times for the first time, decoction liquor merges, Jian Ya Nong Shrink, filtering obtains white paeony root extract 10L standby；
Above-mentioned white paeony root extract, by device 20kgME-l macroporous resin columns, discards efflux；With 20L water rinse resin posts, flushing liquor is discarded；Resin column is eluted with 40% alcohol solvent, 25L eluents are collected, alcohol is reclaimed through Jian Ya Nong Shrink, is dried under reduced pressure.Obtain the refined thing 256g of macroreticular resin standby.
The smart thing of above-mentioned macroreticular resin adds 2 times of amount methanol dissolvings, plus aluminum oxide 1.5kg to mix, and dries, loads percolator, butanone-isopropanol (7:1) elute, collect 9L effluxes, obtain aluminum oxide refined liquid standby；
Above-mentioned aluminum oxide refined liquid, by filling 1.5kgActivated carbon（100 mesh）Post, collects efflux, reclaims whole solvents, dries, obtain absorbent charcoal fine purification thing 98g standby；
Above-mentioned absorbent charcoal fine purification thing 20g adds eluant, eluent butyl acetate-ethanol (8：1) 200mL dissolves, and passes through silicagel column（Diameter 30cm, 4kg), with eluent, stream part is collected, thin layer is inspected, the stream part containing Paeoniflorin, Paeoniflorin and albiflorin, albiflorin is collected respectively, recycling design, then extract, filtering are dissolved with water, filtrate is dried, and Paeoniflorin, Paeoniflorin and albiflorin mixture, albiflorin are obtained respectively.Above-mentioned Paeoniflorin and albiflorin mixture is mixed with above-mentioned absorbent charcoal fine purification thing After conjunction, by silicagel column, with eluent, stream part is collected, thin layer is inspected, carry out repeating refined with this, obtain the 6g of Paeoniflorin 68., the 4g of albiflorin 20., Paeoniflorin and the 4g of albiflorin mixture 5..
Content assaying method：Chromatographic condition and system suitability：It is filler with octadecylsilane chemically bonded silica；The % phosphoric acid solutions of methanol -0. 1（Plus 1% sodium hydroxide solution be adjusted to 4. 2) (34:66) it is mobile phase；Detection wavelength is 230nm.Number of theoretical plate is calculated by Paeoniflorin peak should be not less than 2500.The preparation of reference substance solution：Precision weighs albiflorin reference substance plus every lml 02mg containing albiflorin 0. solution is made in 50% ethanol, produces.The preparation of need testing solution：This product powder 0. lg is taken, it is accurately weighed, put in 1000ml measuring bottles, plus 50% alcohol 95 0ml ultrasounds make dissolving, plus 50% ethanol be shaken up to scale, produce.Determination method：It is accurate respectively to draw above-mentioned photograph product solution and each 10ul of need testing solution, liquid chromatograph is injected, determines, produces.
Determined using foregoing Paeoniflorin and albiflorin content HPLC detection methods：Albiflorin content is 92. 3%.Pill is made in the albiflorin that the above method is obtained, and is taken to the patient with Blood deficiency, daily 2 ball, and the amount containing albiflorin is 45mg in every pill, is continuously taken 20 days, drug effect is obvious.
Embodiment 2:
Root of herbaceous peony 5kg is taken, add water 20L in percolator, immersion carries out diacolation after 2 hours, collect 40L percolates, subtract the dense Shrink of pressure, filtering obtains white paeony root extract 10L standby；
Above-mentioned white paeony root extract, passes through device 10kgD-101 macroporous resin columns, discard efflux；With 45L water rinse resin posts, flushing liquor is discarded；Resin column is eluted with 50% alcohol solvent, 60L eluents are collected, alcohol is reclaimed through Jian Ya Nong Shrink, is dried under reduced pressure.Obtain the refined thing 247g of macroreticular resin standby.
The smart thing of above-mentioned macroreticular resin adds 50% ethanol 250mL to dissolve, plus aluminum oxide lkg is mixed, and is dried, and loads percolator, with ethyl acetate：Methanol（6 :1) it is eluent, collects 6L effluxes, obtain aluminum oxide refined liquid standby；
Above-mentioned aluminum oxide refined liquid collects efflux by the post of the 5kg activated carbons of device 0., and recycling design is dried, obtains absorbent charcoal fine purification thing 99g standby；
Above-mentioned absorbent charcoal fine purification thing 15g adds eluant, eluent（Ethyl acetate：Methanol=8:1) 200mL dissolves, and passes through silicagel column（Diameter 30cm, 4kg), with eluent, stream part is collected, thin layer is inspected, the stream part containing Paeoniflorin, Paeoniflorin and albiflorin, albiflorin is collected respectively, recycling design, then extract, filtering are dissolved with water, filtrate is dried, and Paeoniflorin, Paeoniflorin and albiflorin mixture, albiflorin are obtained respectively.After above-mentioned Paeoniflorin and albiflorin mixture is mixed with above-mentioned absorbent charcoal fine purification thing, pass through silicagel column, with eluent, collect stream part, thin layer is inspected, carry out repeating refined with this, obtain the 6g of Paeoniflorin 70., the 2g of albiflorin 24., Paeoniflorin and the 6g of albiflorin mixture 4..
The albiflorin content HPLC detection methods of embodiment 1 are used to determine albiflorin content for 93. 2%.
Capsule is made in the albiflorin that the above method is obtained, and is taken to patient, daily capsule 3, and the amount containing albiflorin is 40mg in every capsule, is continuously taken 20 days, drug effect is obvious.
Embodiment 3:
Root of herbaceous peony 5kg, plus 50% ethanol is taken to soak 2 hours, refluxing extraction 2 times 1 hour every time, adds 10 times of alcohol for the first time, Second plus 8 times of alcohol, extract solution merge, and ethanol is recovered under reduced pressure, and Jian Ya Nong Shrink, filtering obtains white paeony root extract 20L standby；Above-mentioned white paeony root extract, by device 20kgD-201 macroporous resin columns, discards efflux；With 60L10% alcohol flushing resin posts, flushing liquor is discarded；Resin column is eluted with 40% alcohol solvent, 80L eluents are collected, alcohol is reclaimed through Jian Ya Nong Shrink, is dried under reduced pressure.Obtain the refined thing 239g of macroreticular resin standby.
The smart thing of above-mentioned macroreticular resin is added water 500mL dissolvings, plus aluminum oxide 2kg is mixed, and is dried, is loaded percolator, with methylene chloride-methanol（5 :1) it is eluent, collects 6L effluxes, obtain aluminum oxide refined liquid standby；
Above-mentioned aluminum oxide refined liquid collects efflux by the post of the 8kg activated carbons of device 0., and recycling design is dried, obtains absorbent charcoal fine purification thing 93g standby；
Above-mentioned absorbent charcoal fine purification thing 30g adds eluant dichloromethane-methanol（5 :1) 400mL dissolves, and passes through silicagel column（Diameter 30cm, 5kg), with eluent, stream part is collected, thin layer is inspected, the stream part containing Paeoniflorin, Paeoniflorin and albiflorin, albiflorin is collected respectively, recycling design, then extract, filtering are dissolved with water, filtrate is dried, and Paeoniflorin, Paeoniflorin and albiflorin mixture, albiflorin are obtained respectively.After above-mentioned Paeoniflorin and albiflorin mixture is mixed with above-mentioned absorbent charcoal fine purification thing, pass through silicagel column, with eluent, collect stream part, thin layer is inspected, carry out repeating refined with this, obtain the lg of Paeoniflorin 66., the 2g of albiflorin 21., Paeoniflorin and the 2g of albiflorin mixture 2..
Determined using with the albiflorin content HPLC detection methods of embodiment 1：Albiflorin content is 93. 0%.Granule is made in the albiflorin that the above method is obtained, and is taken to the patient with Blood deficiency, daily 3 bags of granule, and the amount containing albiflorin is 90mg in every bag of granule, is taken 7 days, drug effect is obvious.
Embodiment 4:
Root of herbaceous peony 5kg, plus 70% ethanol is taken to soak 1 hour, refluxing extraction 2 times 1 hour every time, adds 9 times of alcohol, second plus 7 times of alcohol, extract solution merge, ethanol is recovered under reduced pressure, Jian Ya Nong Shrink, filtering obtains white paeony root extract 15L standby for the first time；Above-mentioned white paeony root extract, passes through device 10kgThe post of D-101 macroreticular resins, discards efflux；With 60L10% alcohol flushing resin posts, flushing liquor is discarded；Resin column is eluted with 40% alcohol solvent, 80L eluents are collected, alcohol is reclaimed through Jian Ya Nong Shrink, is dried under reduced pressure.Obtain the refined thing 248g of macroreticular resin standby.
The smart thing of above-mentioned macroreticular resin is added water 500mL dissolvings, plus aluminum oxide 2kg is mixed, and is dried, is loaded percolator, with ethyl acetate-acetone（6 :1) it is eluent, collects 6L effluxes, obtain aluminum oxide refined liquid standby；
Above-mentioned aluminum oxide refined liquid collects efflux by the post of the 0kg activated carbons of device 1., and recycling design is dried, obtains absorbent charcoal fine purification thing 95g standby；
Above-mentioned absorbent charcoal fine purification thing 30g adds eluant ethyl acetate-acetone（6 :1) 400mL dissolves, and passes through silicagel column（Diameter 30cm, 5kg), with eluent, stream part is collected, thin layer is inspected, the stream part containing Paeoniflorin, Paeoniflorin and albiflorin, albiflorin is collected respectively, recycling design, then extract, filtering are dissolved with water, filtrate is dried, and Paeoniflorin, Paeoniflorin and albiflorin mixture, albiflorin are obtained respectively.After above-mentioned Paeoniflorin and albiflorin mixture is mixed with above-mentioned absorbent charcoal fine purification thing, pass through silicagel column, with eluent, collect stream part, thin layer is inspected, carry out repeating refined with this, obtain the 4g of Paeoniflorin 67., the 9g of albiflorin 22., Paeoniflorin and the lg of albiflorin mixture 3.. Determined using with the albiflorin content HPLC detection methods of embodiment 1：Albiflorin content is 92. 2%.Tablet is made in the albiflorin that the above method is obtained, and is taken to patient, daily 4, tablet, and the amount containing albiflorin is 60mg in the tablet of every, is continuously taken 7 days, drug effect is obvious.
Embodiment 5:
Root of herbaceous peony 5kg is taken, add water 30L in percolator, immersion carries out diacolation after 2 hours, collect 50L percolates, subtract the dense Shrink of pressure, filtering obtains white paeony root extract 20L standby；
Above-mentioned white paeony root extract, by the post of the 5kgD-101 macroreticular resins of device 7., discards efflux；With 30L water rinse resin posts, flushing liquor is discarded；Resin column is eluted with 40% alcohol solvent, 60L eluents are collected, alcohol is reclaimed through Jian Ya Nong Shrink, is dried under reduced pressure.Obtain the refined thing 248g of macroreticular resin standby.
The smart thing of above-mentioned macroreticular resin is added water 500mL dissolvings, plus the 5kg of aluminum oxide 1. is mixed, and is dried, is loaded percolator, with chloroform-methanol（7 :1) it is eluent, collects 6L effluxes, obtain aluminum oxide refined liquid standby；
Above-mentioned aluminum oxide refined liquid collects efflux by the post of the 0kg activated carbons of device 1., and recycling design is dried, obtains absorbent charcoal fine purification thing 94g standby；
Above-mentioned absorbent charcoal fine purification thing 30g adds eluant, eluent chloroform-methanol（9 :1) 300mL dissolves, and passes through silicagel column（Diameter 30cm, 5kg), with eluent, stream part is collected, thin layer is inspected, the stream part containing Paeoniflorin, Paeoniflorin and albiflorin, albiflorin is collected respectively, recycling design, then extract, filtering are dissolved with water, filtrate is dried, and Paeoniflorin, Paeoniflorin and albiflorin mixture, albiflorin are obtained respectively.After above-mentioned Paeoniflorin and albiflorin mixture is mixed with above-mentioned absorbent charcoal fine purification thing, pass through silicagel column, with eluent, collect stream part, thin layer is inspected, carry out repeating refined with this, obtain the lg of Paeoniflorin 65., the 2g of albiflorin 23., Paeoniflorin and the 7g of albiflorin mixture 2..
Determined using with the albiflorin content HPLC detection methods of embodiment 1：Albiflorin content is 95. 0%.Capsule is made in the albiflorin that the above method is obtained, and is taken to patient, daily 2, and the amount containing albiflorin is 135mg in every pill, is continuously taken 5 days, drug effect is obvious.
Embodiment 6:
Root of herbaceous peony 5kg, plus 70% ethanol is taken to soak 2 hours, refluxing extraction 3 times 1 hour every time, adds 6 times of 8 times of alcohol, second and third time plus alcohol, extract solution merges for the first time, and ethanol is recovered under reduced pressure, and Jian Ya Nong Shrink, filtering obtains white paeony root extract 10L standby；Above-mentioned white paeony root extract, by the post of device 15kgME-l macroreticular resins, discards efflux；With 45L10% alcohol flushing resin posts, flushing liquor is discarded；Resin column is eluted with 70% alcohol solvent, 80L eluents are collected, alcohol is reclaimed through Jian Ya Nong Shrink, is dried under reduced pressure.Obtain the refined thing 266g of macroreticular resin standby.
The smart thing of above-mentioned macroreticular resin adds 50% methanol 250mL to dissolve, plus aluminum oxide 2kgMix, dry, load percolator, with butyl acetate-methanol（8 :1) it is eluent, collects 5L effluxes, obtain aluminum oxide refined liquid standby；
Above-mentioned aluminum oxide refined liquid collects efflux by the post of the 5kg activated carbons of device 1., and recycling design is dried, obtains absorbent charcoal fine purification thing 97g standby；
Above-mentioned absorbent charcoal fine purification thing 15g adds eluant, eluent butyl acetate-methanol（8 :1) 225mL dissolves, and passes through silicagel column（Diameter 30cm, 4kg), with eluent, stream part is collected, thin layer is inspected, the stream part containing Paeoniflorin, Paeoniflorin and albiflorin, albiflorin is collected respectively, recycling design, then extract, filtering are dissolved with water, filtrate is dried, and Paeoniflorin, Paeoniflorin and albiflorin mixture, albiflorin are obtained respectively.After above-mentioned Paeoniflorin and albiflorin mixture is mixed with above-mentioned absorbent charcoal fine purification thing, pass through silicagel column, with eluent, collect stream part, thin layer is inspected, carry out repeating refined with this, obtain the 7g of Paeoniflorin 66., the 8g of albiflorin 21., Paeoniflorin and the 6g of albiflorin mixture 5..
Determined using with the albiflorin content HPLC detection methods of embodiment 1：Albiflorin content is 93. 5%.Tablet is made in the albiflorin that the above method is obtained, and is taken to patient, daily 2, tablet, and the amount containing albiflorin is 90mg in the tablet of every, is continuously taken 7 days, drug effect is obvious.
Embodiment 7:
The oral liquid being made up of albiflorin, takes to patient, 2 bottles of oral liquid daily, every bottle of 10ml, and the amount containing albiflorin is 105mg in every bottle, is continuously taken 15 days, drug effect is obvious.
Embodiment 8:
The injection being made up of albiflorin, is injected to the patient with Blood deficiency, and the amount containing albiflorin is 240mg in daily injection, and every injection contains albiflorin 120mg, daily injection 2, is injected 7 days, drug effect is obvious.

Claims (31)

  1. Claims
    1. application of the albiflorin in medicament for enriching blood is prepared.
    2. the application as described in claim 1, it is characterised in that application of the albiflorin in the medicine for preparing treatment Blood deficiency.
    3. application as claimed in claim 1 or 2, it is characterised in that amount of the per unit preparation containing albiflorin is 90mg 270mg according to daily preparation total amount.
    4. application as claimed in claim 3, it is characterised in that amount of the per unit preparation containing albiflorin is 120mg or 180mg or 240mg according to daily preparation total amount.
    5. a kind of albiflorin for medicament for enriching blood, it is characterised in that albiflorin is made by the following method：
    Step 1:Extract：White Peony Root is extracted using water or alcohol as solvent through conventional method, and extract solution obtains white paeony root extract standby through dense Shrink, filtering；
    Step 2:Macroreticular resin is refined：White paeony root extract, by macroporous resin column, discards efflux；Resin column is rinsed with 0 20% alcoholic solvent, flushing liquor is discarded；Resin column is eluted with 30 70% alcoholic solvents, eluent is collected, alcohol is reclaimed through Jian Ya Nong Shrink, is dried under reduced pressure, macroreticular resin is obtained and refines thing.
    Step 3:Aluminum oxide is refined：The smart thing of macroreticular resin is taken to add water or alcohol dissolving, root of herbaceous peony macroreticular resin refines thing and adds the aluminum oxide of 2-8 times of weight to mix, dries, and loads percolator, with eluent, collects whole effluxes, obtains aluminum oxide refined liquid standby；
    Step 4:Absorbent charcoal fine purification：Aluminum oxide refined liquid collects efflux by activated-charcoal column, reclaims whole solvents, dries, obtain absorbent charcoal fine purification thing standby；
    Step 5:Silica gel is refined：8 20 volume eluant, eluents are added to dissolve absorbent charcoal fine purification thing, pass through silicagel column, with eluent, stream part is collected, thin layer is inspected, the stream part containing Paeoniflorin, Paeoniflorin and albiflorin, albiflorin is collected respectively, whole solvents are reclaimed, then respectively with each stream part extract of water dissolving, filtering, filtrate is dried, and Paeoniflorin, Paeoniflorin and albiflorin mixture, albiflorin are obtained respectively.
    Step 6:Paeoniflorin and the albiflorin mixture presses the method for above-mentioned steps 5, repeats refined, obtains Paeoniflorin, albiflorin.
    6. being used for the albiflorin of medicament for enriching blood as claimed in claim 5, it is characterised in that in step 1, root of herbaceous peony Extraction solvent is water, and extracting method is diacolation, 14 times of medicinal material amount/hours of flow velocity.
    7. the albiflorin for medicament for enriching blood as described in claim 5, it is characterised in that the macroreticular resin is one kind in ME-1, D- 101, DA- 201, D- 301, AB-8.
    8. being used for the albiflorin of medicament for enriching blood as claimed in claim 5, it is characterised in that in step 2, the alcoholic solution is the aqueous solution of methanol, ethanol or propyl alcohol.
    9. it is used for the albiflorin of medicament for enriching blood as claimed in claim 5, it is characterised in that in step 2, macroreticular resin consumption For root of herbaceous peony 1-3 times of weight, flow velocity is 0. 53 times of amount of resin/hours；0 20% alcoholic solvent of the resin column with 15 macroreticular resin times of weight is rinsed, flushing liquor is discarded；The eluent of collection is 30 70% alcoholic solvents of 38 macroreticular resin times of weight.
    10. being used for the albiflorin of medicament for enriching blood as claimed in claim 5, it is characterised in that in step 3, root of herbaceous peony macroreticular resin refines thing and adds the aluminum oxide of 4 times of weight to mix；Collect the 4-8 times of weight that eluent is aluminum oxide.
    11. it is used for the albiflorin of medicament for enriching blood as claimed in claim 5, it is characterised in that activated-charcoal column refines thing 1-6 times of weight equivalent to root of herbaceous peony macroreticular resin；The absorbent charcoal fine purification thing of preparation is more than 95% containing Paeoniflorin and albiflorin sum.
    12. it is used for the albiflorin of medicament for enriching blood as claimed in claim 12, it is characterised in that activated-charcoal column refines the times of weight of thing 2 equivalent to root of herbaceous peony macroreticular resin.
    13. it is used for the albiflorin of medicament for enriching blood as claimed in claim 5, it is characterised in that with acetate-methanol 3-9 in step 3 and step 5:1 elution.
    14. it is used for the albiflorin of medicament for enriching blood as claimed in claim 13, it is characterised in that step 3 and step 5 acetate-methanol are 6: 1.
    15. it is used for the albiflorin of medicament for enriching blood as claimed in claim 13, it is characterised in that acetate-methanol is replaced by following solvents：
    Liquid alcohols：Methanol, ethanol, propyl alcohol, isopropanol；Liquid esters：Methyl formate, Ethyl formate, methyl acetate, ethyl acetate, propyl acetate, butyl acetate, pentyl acetate；Liquid ketone：Acetone, butanone, pentanone；Liquid alkane class：Petroleum ether, pentane, hexane, heptane, octane；Liquid ethers：Ether, propyl ether, butyl, methyl tertiary butyl ether(MTBE)；Liquified Halon class：Chloroform, dichloromethane, carbon tetrachloride, iodomethane, bromoethane；
    Above-mentioned solvent carries out selecting a selection according to the principle suitable with acetate-methanol polarity or multi-solvents are mixed.
    16. the albiflorin for medicament for enriching blood as described in claim 5-15 is any, albiflorin content 92%.
    17. the preparation method of a kind of albiflorin for medicament for enriching blood, it is characterised in that this method comprises the following steps：
    Step 1:Extract：White Peony Root is extracted using water or alcohol as solvent through conventional method, and extract solution obtains white paeony root extract standby through dense Shrink, filtering；
    Step 2:Macroreticular resin is refined：White paeony root extract, by macroporous resin column, discards efflux；Resin column is rinsed with 0 20% alcoholic solvent, flushing liquor is discarded；Resin column is eluted with 30 70% alcoholic solvents, eluent is collected, alcohol is reclaimed through Jian Ya Nong Shrink, is dried under reduced pressure, macroreticular resin is obtained and refines thing.
    Step 3:Aluminum oxide is refined：The smart thing of macroreticular resin is taken to add water or alcohol dissolving, root of herbaceous peony macroreticular resin refines thing and adds the aluminum oxide of 2-8 times of weight to mix, dries, and loads percolator, with eluent, collects whole effluxes, obtains aluminum oxide refined liquid standby；
    Step 4:Absorbent charcoal fine purification：Aluminum oxide refined liquid collects efflux by activated-charcoal column, reclaims whole solvents, dries, Obtain absorbent charcoal fine purification thing standby；
    Step 5:Silica gel is refined：8 20 volume eluant, eluents are added to dissolve absorbent charcoal fine purification thing, pass through silicagel column, with eluent, stream part is collected, thin layer is inspected, the stream part containing Paeoniflorin, Paeoniflorin and albiflorin, albiflorin is collected respectively, whole solvents are reclaimed, then respectively with each stream part extract of water dissolving, filtering, filtrate is dried, and Paeoniflorin, Paeoniflorin and albiflorin mixture, albiflorin are obtained respectively.
    Step 6:Paeoniflorin and the albiflorin mixture presses the method for above-mentioned steps 5, repeats refined, obtains Paeoniflorin, albiflorin.
    18. being used for the albiflorin of medicament for enriching blood as claimed in claim 17, it is characterised in that in step 1, root of herbaceous peony Extraction solvent is water, and extracting method is diacolation, 14 times of medicinal material amount/hours of flow velocity.
    19. it is used for the albiflorin of medicament for enriching blood as claimed in claim 17, it is characterised in that the macroreticular resin is one kind in ME-1, D- 101, DA- 201, D- 301, AB-8.
    20. being used for the albiflorin of medicament for enriching blood as claimed in claim 17, it is characterised in that in step 2, the alcoholic solution is the aqueous solution of methanol, ethanol or propyl alcohol.
    21. being used for the albiflorin of medicament for enriching blood as claimed in claim 17, it is characterised in that in step 2, macroreticular resin consumption is root of herbaceous peony 1-3 times of weight, and flow velocity is 0. 53 times of amount of resin/hours；0 20% alcoholic solvent of the resin column with 15 macroreticular resin times of weight is rinsed, flushing liquor is discarded；The eluent of collection is 30 70% alcoholic solvents of 38 macroreticular resin times of weight.
    22. being used for the albiflorin of medicament for enriching blood as claimed in claim 17, it is characterised in that in step 3, root of herbaceous peony macroreticular resin refines thing and adds the aluminum oxide of 4 times of weight to mix；Collect the 4-8 times of weight that eluent is aluminum oxide.
    23. it is used for the albiflorin of medicament for enriching blood as claimed in claim 17, it is characterised in that activated-charcoal column refines thing 1-6 times of weight equivalent to root of herbaceous peony macroreticular resin；The absorbent charcoal fine purification thing of preparation is more than 95% containing Paeoniflorin and albiflorin sum.
    24. it is used for the albiflorin of medicament for enriching blood as claimed in claim 23, it is characterised in that activated-charcoal column refines the times of weight of thing 2 equivalent to root of herbaceous peony macroreticular resin.
    25. it is used for the albiflorin of medicament for enriching blood as claimed in claim 17, it is characterised in that with acetate-methanol 3-9 in step 3 and step 5:1 elution.
    26. it is used for the albiflorin of medicament for enriching blood as claimed in claim 25, it is characterised in that step 3 and step 5 acetate-methanol are 6: 1.
    27. it is used for the preparation method of the albiflorin of medicament for enriching blood as claimed in claim 25, it is characterised in that acetate-methanol is replaced by following solvents：
    Liquid alcohols：Methanol, ethanol, propyl alcohol, isopropanol；Liquid esters：Methyl formate, Ethyl formate, methyl acetate, Ethyl acetate, propyl acetate, butyl acetate, pentyl acetate；Liquid ketone：Acetone, butanone, pentanone；Liquid alkane class：Petroleum ether, pentane, hexane, heptane, octane；Liquid ethers：Ether, propyl ether, butyl, methyl tertiary butyl ether(MTBE)；Liquified Halon class：Chloroform, dichloromethane, carbon tetrachloride, iodomethane, bromoethane；
    Above-mentioned solvent carries out selecting a selection according to the principle suitable with acetate-methanol polarity or multi-solvents are mixed.
    28. the preparation method of the albiflorin for medicament for enriching blood as described in claim 17-27 is any, it is characterised in that albiflorin content 92%.
    29.-kind it is used for the pharmaceutical preparation enriched blood, it is characterised in that amount of the per unit preparation containing albiflorin is 90mg 270mg according to daily preparation total amount.
    30. preparation as claimed in claim 29, it is characterised in that said preparation clinic acceptable pill, capsule, granule, tablet, oral liquid or injection.
    31. application of the albiflorin in preparing for medicament for enriching blood as described in any in claim 5-16,29,30.
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Publication number Priority date Publication date Assignee Title
WO2021098826A1 (en) * 2019-11-22 2021-05-27 张作光 Use of albiflorin or paeoniflorin in the prevention and/or treatment of renal anemia

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