CN105368719B - One plant of Trichoderma for preventing and treating clover anthracnose and its application - Google Patents
One plant of Trichoderma for preventing and treating clover anthracnose and its application Download PDFInfo
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Abstract
The Trichoderma for preventing and treating clover anthracnose the invention discloses one plant and its application.The present invention provides a kind of Trichoderma, the deposit number of the Trichoderma (Trichoderma koningii) is CGMCC No.11221.The present invention also provides a kind of complex micro organism fungicide, the active constituent of the complex micro organism fungicide includes Trichoderma as described above and Bacillus subtillis (Bacillus subtilis).The present invention also provides the application of Trichoderma as described above or microbial bacterial agent as described above in the prevention and treatment of clover anthracnose.Through the above technical solutions, the present invention can effectively prevent the generation of clover anthracnose.
Description
Technical field
The present invention relates to microorganism fields, and in particular, to a kind of Trichoderma (Trichoderma koningii) is contained
The microbial bacterial agent of the Trichoderma and they clover anthracnose prevention and treatment in application.
Background technique
Clover Medicago sativa L. is perennial leguminous plant, " King of Pasture " laudatory title is known as, because its nutrition is rich
Rich, yield height, palatability are good, and protein content is high and containing multivitamin needed for growth of animal in addition, therefore in herding
It plays an important role in industry development.
Clover anthracnose is one of alfalfa cultivation Major Diseases, seriously affects clover quality and yield.Clover anthrax
Most susceptible disease at sick main harm above-ground plant parts, especially near-earth vines.Just occur yellowish-brown dot at vines, after gradually
It is expanded into oblong, when scab expands, mutually converges, ring stem one week, causes branch aggrieved withered.Clover anthracnose is not
Few country has been distributed more widely, destructive disease, also has generation in many areas in China.The known disease for causing clover anthracnose
Opportunistic pathogen is mainly 3 kinds of common thorn disk spores, is clover thorn disk spore (Colletotrichum trifolii) respectively, destroys thorn
Disk spore (Colletotrichum destructivum) and tack thorn disk spore (Colletotrichum truncatum).Wherein
Tack thorn disk spore blazons all over the world, infects a variety of leguminous plants, also includes clover wherein being no lack of various leguminous forages.
In recent years, since the pollution problem of chemical pesticide is on the rise, biological control because its with pollution-free, noresidue,
The advantages that ecological environment and safer human health and be increasingly subject to people's attention, wherein antagonistic microbe is raw in plant disease
Application in object prevention and treatment is concerned.Trichoderma is one of biocontrol microorganisms most competitive in numerous antagonistic microbes, is certainly
A kind of filamentous fungi being widely present in right boundary, adaptable antagonist that is strong, generating is more, parasitics is wide and effect on environment
Small equal various features, the application potential in disease flocking biocontrol is paid attention to increasingly, and constantly has breakthrough.Currently, the antagonism of research
There are many Trichoderma type, mainly include Trichoderma harzianum (Trichoderma harzianum), hook-shaped trichoderma (Trichoderma
Hamatum), long shoot trichoderma (Trichoderma longibrachiatum) and Trichoderma viride (Trichoderma viride)
Deng.Known Trichoderma biological control mechanism mainly includes competing nutrition and the space, the hyperparasite to pathogen, generation of pathogen
Antibiotic inhibits or kills pathogen and plant is promoted to induce autoimmune etc..Controlling object is mainly that soil-borne pathogen causes
Plant disease, such as beans root rot (Fusarium graminerarum) and anthracnose (Colletotrichum
Truncatum), cotton wilt (Fusarium oxysporium) and the climing wilt disease of watermelon (Didymella bryoniae)
Deng.
Bacillus subtillis (Bacillus subtilis) is used as a kind of gram-positive bacterium, is distributed in nature
Extensively, a variety of antibiotic and enzyme with bacteriostatic activity can be secreted, there is very strong antibacterial disease prevention ability.Its biological control
Mechanism mainly includes with pathogen competition for nutrients, secreting antibacterial material and enzyme by colonizing the rhizosphere in plant, body surface or in vivo
Class etc. inhibits the growth of pathogen, while plant growth and induction plant can also be promoted to generate resistance etc..Controlling object is main
For plant disease caused by filamentous fungi, such as cotton wilt (Fusarium oxysporium) and verticillium wilt
(Verticillium dahliae), root rot (Fusarium graminerarum), rice sheath blight disease
(Rhizoctonia solani) and graw mold of tomato (Botrytis cinerea) etc..
Now, environmental pollution is got worse, and biological control has great potentiality for the improvement of clover anthracnose, is utilized
Biocontrol microorganism is screened to the antagonism of clover Anthracnose Pathogen fungi, and true using microbial bacterial agent collective effect cause of disease
Bacterium is the effective way of clover anthracnose prevention and treatment.
Summary of the invention
The present inventor has screened one plant of Trichoderma, it is found that it can inhibit clover Anthracnose Pathogen bacterium significantly
Growth, effectively prevent the generation of clover anthracnose, result in the present invention.
The present invention provides a kind of Trichoderma, which is trichodermaharzianum (Trichoderma koningii
(TKmx)), deposit number is CGMCC No.11221.
The present invention also provides a kind of complex micro organism fungicide, the complex micro organism fungicide active constituent includes as above
The Trichoderma and article number is the Bacillus subtillis (Bacillus subtilis) of ACCC 03221.
The present invention also provides Trichodermas as described above or complex micro organism fungicide as described above in clover anthracnose
Purposes in prevention and treatment.
Through the above technical solutions, Trichoderma provided by the invention or complex micro organism fungicide have clover anthracnose
Higher preventive effect, and have the advantages that environmentally protective.
Other features and advantages of the present invention will the following detailed description will be given in the detailed implementation section.
Biomaterial preservation
Trichoderma (Trichoderma koningii) of the invention is the present inventor from Hebei province Huanghua City lucerne
It separated in Mu planting site soil, screen acquisition, deposit number is CGMCC No.11221, and the deposit date is Augusts 11 in 2015
Day, depositary institution is China Committee for Culture Collection of Microorganisms's common micro-organisms center, and address is located at Chaoyang District, Beijing City
The institute 3 of North Star West Road 1, classification naming are trichodermaharzianum (Trichoderma koningii).
Specific embodiment
Detailed description of the preferred embodiments below.It should be understood that described herein specific
Embodiment is merely to illustrate and explain the present invention, and is not intended to restrict the invention.
The present invention provides a kind of Trichoderma, which is trichodermaharzianum (Trichoderma koningii
(TKmx)), deposit number is CGMCC No.11221.
The present invention also provides a kind of complex micro organism fungicide, the complex micro organism fungicide active constituent includes as above-mentioned
The Trichoderma and article number is the Bacillus subtillis (Bacillus subtilis) of ACCC 03221.
Wherein, relative to the microbial bacterial agent of 1g, the content of the Trichoderma can be 105-109CFU, preferably 106-
108CFU。
Wherein, the CFU of the Trichoderma and Bacillus subtillis ratio is preferably 1:(10-20).
Wherein, the microbial bacterial agent can preparation method as follows be prepared:
(1) by the Trichoderma that deposit number is CGMCC No.11221 and the Ko subtilis bar that article number is ACCC 03221
The inoculated and cultured in activation medium obtains respective activated spawn to bacterium (Bacillus subtilis) strain respectively；
(2) activated spawn of Trichoderma is cultivated in trichoderma liquid fermentation medium, while by Bacillus subtillis
Activated strains cultivated in its liquid fermentation medium, the bacterium solution after obtaining respective fermentation；
(3) Trichoderma obtained step (2) and Bacillus subtillis bacterium solution are respectively through inert carrier absorption, drying and powder
It is broken, obtain the bacterium powder of each strain；
(4) the bacterium powder of Trichoderma and Bacillus subtillis is mixed in proportion, obtains complex micro organism fungicide.
Wherein, in step (1), strain state is not particularly limited after the activation culture condition of strain and culture, can be with
For typical conditions in Trichoderma and Bacillus subtillis incubation, such as cultivation temperature can be 28-32 DEG C, and incubation time can
Think 1-5 days.During culture, the state of activated spawn can be determined by conventional method, such as micro- sem observation.
Wherein, in step (2), the viable bacteria concentration in material after fermentation condition and fermentation is not specially required, Ke Yiwei
Common condition and concentration in Trichoderma and Bacillus subtillis fermentation process, as a kind of currently preferred embodiment,
In step (2), the condition of fermentation makes the viable bacteria concentration in the material after fermentation be (5-500) × 108CFU/mL, it is preferred at this
Under viable bacteria concentration in embodiment, the raw material that the bacterium solution after the fermentation of acquisition is prepared as microbial bacterial agent is used.Such as it sends out
The time of ferment can be 20-200 hours, and temperature can be 28-32 DEG C.
Wherein, the activation medium can be the conventional various cultures that can cultivate Trichoderma and Bacillus subtillis
Base, for example including but be not limited in PDA culture medium, beef-protein medium, broth bouillon and LB culture medium at least
It is a kind of.
Wherein, the trichoderma liquid fermentation medium can expand Trichoderma number for conventional various can ferment
Fluid nutrient medium, such as the fluid nutrient medium can contain starch, yeast powder, at least one of corn pulp；And the liquid
Culture medium can also contain the ZnSO of 0.01-0.02%4With the MgSO of 0.01-0.03%4, when supplementing Trichoderma fast-growth
Required inorganic salts.
Wherein, the Bacillus subtillis liquid fermentation medium can expand withered grass bud for conventional various can ferment
The fluid nutrient medium of born of the same parents' bacillus number, for example, the fluid nutrient medium can containing cornstarch, yeast extract, groundnut meal,
At least one of cottonseed meal；And the fluid nutrient medium can also contain 0.2-0.5% ammonium sulfate, 0.02-0.05% sulfuric acid
Magnesium, 0.01-0.05% sodium chloride, required inorganic salts when supplementing Bacillus subtillis fast-growth.
Wherein, as a kind of embodiment specifically preferred according to the invention, with the total of the trichoderma liquid fermentation medium
It on the basis of weight, is calculated with weight ratio, the trichoderma liquid fermentation medium contains 2.0-3.5% starch, 1.0-2.0% ferment
Female powder, 4.0-8.0% corn pulp, the CaCO of 0.1-0.5%3, the ZnSO of 0.01-0.02%4With the MgSO of 0.01-0.03%4。
Wherein, which can also contain the water having a margin and/or other ingredients.In the preferred embodiment, trichoderma on the one hand can be allowed
Bacterium fast-growth during the fermentation generates sufficient amount of chlamydospore, the material conduct after the fermentation on the other hand obtained
Microbial bacterial agent uses, and can preferably apply in the prevention and treatment of clover anthracnose.
Wherein, as a kind of embodiment specifically preferred according to the invention, the Bacillus subtillis liquid fermentation medium
Contain 1-1.5% cornstarch, 1-1.5% groundnut meal, 0.2-0.5% ammonium sulfate, 0.1-0.4% calcium carbonate, 0.02-
0.05% magnesium sulfate and 0.01-0.05% sodium chloride.Wherein, which can also contain the water having a margin and/or other ingredients.
In the preferred embodiment, it on the one hand can allow fast-growth in Bacillus subtillis fermentation process, generate sufficient amount of brood cell,
On the other hand the material after the fermentation obtained is used as microbial bacterial agent, can preferably be answered in the prevention and treatment of clover anthracnose
With.
Wherein, the bacterium solution after fermentation can be used directly as microbial bacterial agent, can also be by including carrier adsorption, drying
It is dry, crush and/or be granulated and etc. be further processed into more convenient storage the microbial bacterial agent of dosage form use.Inert carrier
Dosage can make viable bacteria content in Trichoderma bacterium powder reach (2-5) × 108CFU/g, viable bacteria contains in Bacillus subtillis bacterium powder
Amount respectively reaches (2-10) × 109CFU/g.Trichoderma and Bacillus subtillis bacterium powder are mixed；So that the Trichoderma and institute
The CFU ratio for stating Bacillus subtillis is 1:(10-20).The preparation of two kinds of microbial inoculums is not limited in complex micro organism fungicide preparation
Aforementioned proportion can be the mixing of other ratios.
Wherein, the inert carrier may include calcium carbonate, loess, diatomite, bentonite, talcum powder, mica powder, kaolinite
At least one of soil and calcite in powder.
The present invention also provides Trichodermas as described above or complex micro organism fungicide as described above in clover anthracnose
Purposes in prevention and treatment.
Wherein, in a preferred embodiment of the invention, multiple by Trichoderma as described above or as described above
Microbial bacterial agent is closed to apply in the prevention and treatment of clover anthracnose.
Wherein, in a kind of embodiment specifically preferred according to the invention, in the using in farmland Trichoderma or such as of plantation clover
The upper complex micro organism fungicide；Wherein, it plants Trichoderma or complex micro organism fungicide as described above and thin before clover
Soil mixes, and applies in planting soil；Or in the clover seeding stage, by the Trichoderma or complex micro organism fungicide as described above
It mixes, is applied in planting soil with fine earth.
Present invention be described in more detail by the following examples:
Embodiment 1
The present embodiment is for illustrating the culture of Trichoderma of the invention and the preparation of microbial bacterial agent.
The Trichoderma (Trichoderma koningii (TKmx)) that deposit number is CGMCC No.11221 is inoculated into
Activation medium PDA culture medium (takes the potato 20g after peeling to boil 20 minutes and takes juice, by juice and 2g glucose, 1.5g agar
It is made into 100ml, pH6.8-7.0 with water, is packed into 2 eggplant bottles, each 50ml, inclined-plane processed after sterilizing) on, it is cultivated 6 days at 28 DEG C.
By activated inclined-plane Trichoderma strain be seeded to sterilizing liquid fermentation medium (by weight, containing 2.5% starch,
1.5% yeast powder, 5.0% corn pulp, 0.4% CaCO3, 0.01% ZnSO4, 0.03% MgSO4), 28 DEG C of cultures 144
Hour, until 90% mycelia all forms chlamydospore, obtain Trichoderma liquor.
The above-mentioned Trichoderma liquor of 10000mL is centrifuged, wet thallus is collected, is sufficiently mixed with appropriate carrier diatomaceous earth.It will
Uniformly mixed material is placed in 50 DEG C of baking ovens, is toasted 4-7 hour, is made its water content less than 5%.It is counted using plate culture
Method detects number of viable in bacterium powder, and then adjusting the trichoderma viable count up to containing in every gram of microbial inoculum using diatomite is 500,000,000, institute
Obtained material is the Trichoderma microbial bacterial agent of the present embodiment.
Embodiment 2
The present embodiment is used to illustrate the preparation of complex micro organism fungicide of the invention.
The Bacillus subtillis (Bacillus subtilis) that article number is ACCC 03221 is inoculated into LB culture medium
On (NaCl the and 15g/L agar of the yeast extract of tryptone, 5g/L containing 10g/L, 10g/L) inclined-plane, cultivated at 30 DEG C
18 hours, the strain activated.The liquid fermentation medium that activated strain is seeded to sterilizing (by weight, is contained
1% cornstarch, 1.5% groundnut meal, 0.5% ammonium sulfate, 0.4% calcium carbonate, 0.03% magnesium sulfate, 0.02% sodium chloride)
In, being cultivated in 35 DEG C to viable bacteria concentration is 5-10 × 109CFU/mL obtains Bacillus subtillis bacterium solution.
The above-mentioned Bacillus subtillis bacterium solution of 10000mL is centrifuged or is filtered, collect wet thallus, then with appropriate carrier
Diatomite mixing.Uniform material will be sufficiently mixed to be placed in 60 DEG C of baking ovens, 4-7 hour is toasted, be lower than its water content
5%.Using thalline quantity in plate culture counting method detection bacterium powder, then adjusted using diatomite until containing in every gram of microbial inoculum
Bacillus subtillis viable count be 2,000,000,000.
Trichoderma bacterium powder and the present embodiment that embodiment 1 obtains are obtained into Bacillus subtillis bacterium powder by weight 1:3
Mixing, obtained material is the complex micro organism fungicide of the present embodiment.
Comparative example 1
Complex micro organism fungicide is prepared according to the method for embodiment 2, unlike, Trichoderma uses document (Xiao Ye
Deng antagonism of the Trichoderma to several plant pathogen, " Agricultural University Of Hunan's journal ", 01 phase in 2007) in Kazakhstan thatch wood
Mould (Trichoderma harzianum) No. 26 numbers.
Comparative example 2
Complex micro organism fungicide is prepared according to the method for embodiment 2, unlike, the wax for being ACCC11109 with article number
Shape bacillus (Bacillus cereus) replaces the Bacillus subtillis (Bacillus that article number is ACCC 03221
subtilis)。
Testing example 1
This testing example measures the effect of microbial bacterial agent of the invention in prevention and treatment clover anthracnose in field trials
Fruit.
Experimental plot is located at base tested by the herding of the Langfang City Chinese Academy of Agricultural Sciences of Hebei province, will die of the clover of anthracnose
The tissue mashing of plant, with etc. weight water impregnate 48 hours, the seed-soaking liquid containing anthracnose pathogenic bacteria is obtained by filtration.It will be pale reddish brown
Clover (middle lucerne 1 is provided, catalog number 00068 by national planting forage grass mid-term library) seed is soaked in above-mentioned containing charcoal
24 hours in the seed-soaking liquid of subcutaneous ulcer disease pathogenic bacteria, the dosage of seed-soaking liquid is enough to flood alfalfa seed, the seed after being impregnated.
Alfalfa seed after immersion is seeded in farmland, trichoderma, the embodiment 2 that embodiment 1 is prepared are set
At complex micro organism fungicide that the complex micro organism fungicide and comparative example 1,2 being prepared are prepared and blank control five
Reason, respectively sets 3 repetitions, totally 15 cells, random alignment.After clover emerges 10 days, embodiment 1 is prepared respectively wood
The complex micro organism fungicide that mould agent, embodiment 2 and comparative example 1,2 are prepared is sufficiently mixed with sterilizing fine earth, is made in fine earth
Viable count content reaches 2 × 106CFU/g, with handling, every square metre of 100g fine earth uniformly applies farmland, with equivalent weight diatom
Soil carries out identical soil of mixing and handles as control.Field management is conventional.Clover anthracnose incidence is investigated after growth 40 days.Clover
The anthracnose state of an illness is divided into 5 ranks, and be immunized (0 grade): disease refers to 0；Disease-resistant (1 grade): disease refers to 0.1-20.0；Resistance to disease (2 grades): disease refers to
20.1-40.0；Susceptible (3 grades): disease refers to 40.1-60.0；Height sense (4 grades): disease refers to 60.0 or more or completely withered.Disease index=
∑ (onset grade typical value × this grade strain number)/(the morbidity other typical value × total strain number of most heavy duty) × 100；Control efficiency=
(control disease index-processing disease index)/control disease index × 100%.The results are shown in Table 1.
Table 1
| Processing | Disease index | Control efficiency |
| Control | 90.31±5.74 | - |
| 1 trichoderma of embodiment | 19.65±3.17 | 78.24% |
| 2 complex micro organism fungicide of embodiment | 15.82±2.85 | 82.48% |
| 1 complex micro organism fungicide of comparative example | 35.28±5.03 | 60.93% |
| 2 complex micro organism fungicide of comparative example | 29.63±4.52 | 67.19% |
By the data of table 1 as it can be seen that trichoderma bacteria agent of the invention, complex micro organism fungicide all have clover anthracnose
Significant control efficiency, and complex micro organism fungicide of the invention is higher than the preventive effect of trichoderma bacteria agent.
The preferred embodiment of the present invention has been described above in detail, still, during present invention is not limited to the embodiments described above
Detail within the scope of the technical concept of the present invention can be with various simple variants of the technical solution of the present invention are made, this
A little simple variants all belong to the scope of protection of the present invention.
It is further to note that specific technical features described in the above specific embodiments, in not lance
In the case where shield, can be combined in any appropriate way, in order to avoid unnecessary repetition, the present invention to it is various can
No further explanation will be given for the combination of energy.
In addition, various embodiments of the present invention can be combined randomly, as long as it is without prejudice to originally
The thought of invention, it should also be regarded as the disclosure of the present invention.
Claims (11)
1. a kind of Trichoderma, it is characterised in that: the Trichoderma is trichodermaharzianum bacterium (Trichoderma koningii), preservation
Number is CGMCC No.11221.
2. a kind of complex micro organism fungicide, it is characterised in that: the complex micro organism fungicide active constituent contains claim 1
The Trichoderma and article number is the Bacillus subtillis (Bacillus subtilis) of ACCC 03221.
3. complex micro organism fungicide according to claim 2, it is characterised in that: described relative to the microbial bacterial agent of 1g
The content of Trichoderma is 105-109CFU。
4. complex micro organism fungicide according to claim 3, it is characterised in that: described relative to the microbial bacterial agent of 1g
The content of Trichoderma is 106-108CFU。
5. the complex micro organism fungicide according to any one of claim 2-4, it is characterised in that: the Trichoderma and institute
The CFU ratio for stating Bacillus subtillis is 1:(10-20).
6. the complex micro organism fungicide according to any one of claim 2-4, it is characterised in that: the microbial bacterial agent packet
The preparation method for including following steps is prepared:
(1) Bacillus subtillis (Bacillus for being ACCC 03221 by Trichoderma described in claim 1 and article number
Subtilis) strain is inoculated in activation medium respectively and cultivates to obtain respective activated spawn；
(2) activated spawn of Trichoderma is cultivated in trichoderma liquid fermentation medium, while by the work of Bacillus subtillis
Change bacterial strain to cultivate in its liquid fermentation medium, the bacterium solution after obtaining respective fermentation；
(3) Trichoderma obtained step (2) and Bacillus subtillis bacterium solution through inert carrier absorption, drying and crush respectively,
Obtain the bacterium powder of each strain；
(4) the bacterium powder of Trichoderma and Bacillus subtillis is mixed in proportion, obtains complex micro organism fungicide.
7. complex micro organism fungicide according to claim 6, it is characterised in that: the dosage of inert carrier makes Trichoderma bacterium
Viable bacteria content reaches (2-5) × 10 in powder8CFU/g, viable bacteria content reaches (2-10) × 10 in Bacillus subtillis bacterium powder9CFU/
G mixes the bacterium powder of Trichoderma and Bacillus subtillis, so that the CFU of the Trichoderma and Bacillus subtillis ratio is 1:
(10-20)。
8. complex micro organism fungicide according to claim 6, it is characterised in that: the inert carrier includes calcium carbonate, Huang
At least one of soil, diatomite, talcum powder, mica powder, kaolin and calcite in powder.
9. complex micro organism fungicide according to claim 6, it is characterised in that: with the trichoderma liquid fermentation medium
Total weight on the basis of, by weight, the trichoderma liquid fermentation medium contains 2.0-3.5% starch, 1.0-2.0% ferment
Female powder, 4.0-8.0% corn pulp, 0.1-0.5% CaCO3, 0.01-0.02% ZnSO4With the MgSO of 0.01-0.03%4。
10. complex micro organism fungicide according to claim 6, it is characterised in that: with Bacillus subtillis liquid hair
On the basis of the total weight of ferment culture medium, by weight, liquid fermentation medium used in Bacillus subtillis contains 1-1.5% jade
Rice starch, 1-1.5% groundnut meal, 0.2-0.5% ammonium sulfate, 0.1-0.4% calcium carbonate, 0.02-0.05% magnesium sulfate and
0.01-0.05% sodium chloride.
11. microbial bacterial agent described in any one of Trichoderma described in claim 1 or claim 2-10 is in clover charcoal
Purposes in the prevention and treatment of subcutaneous ulcer disease.
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| CN111838190A (en) * | 2019-04-24 | 2020-10-30 | 山东大学苏州研究院 | Biocontrol microbial inoculum for preventing and treating stem base rot and gummosis as well as preparation method and application thereof |
| CN114747478B (en) * | 2021-01-15 | 2023-10-13 | 冯永德 | Composite breeding method for pasture |
| CN113443944A (en) * | 2021-08-02 | 2021-09-28 | 山西农业大学高寒区作物研究所(山西省农业科学院高寒区作物研究所) | Special organic compound fertilizer for alfalfa |
Citations (3)
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|---|---|---|---|---|
| US5314691A (en) * | 1989-11-09 | 1994-05-24 | The Regents Of The University Of California | Biological control of phytophthora on plants and in soil with Myrothecium roridum ATCC 20963, 20964 or 20965 |
| CN102823467A (en) * | 2012-09-21 | 2012-12-19 | 农业部环境保护科研监测所 | Method for controlling legume root rot by using trichoderma viride |
| CN104498369A (en) * | 2014-12-05 | 2015-04-08 | 中国农业科学院植物保护研究所 | Trichoderma koningii and bacterial agent containing same and application thereof in prevention of cylindrocarpon destructans |
-
2015
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5314691A (en) * | 1989-11-09 | 1994-05-24 | The Regents Of The University Of California | Biological control of phytophthora on plants and in soil with Myrothecium roridum ATCC 20963, 20964 or 20965 |
| CN102823467A (en) * | 2012-09-21 | 2012-12-19 | 农业部环境保护科研监测所 | Method for controlling legume root rot by using trichoderma viride |
| CN104498369A (en) * | 2014-12-05 | 2015-04-08 | 中国农业科学院植物保护研究所 | Trichoderma koningii and bacterial agent containing same and application thereof in prevention of cylindrocarpon destructans |
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