CN105368700B - Pit microorganism in situ culture apparatus and method - Google Patents
Pit microorganism in situ culture apparatus and method Download PDFInfo
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- CN105368700B CN105368700B CN201510931179.7A CN201510931179A CN105368700B CN 105368700 B CN105368700 B CN 105368700B CN 201510931179 A CN201510931179 A CN 201510931179A CN 105368700 B CN105368700 B CN 105368700B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/12—Well or multiwell plates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/02—Membranes; Filters
- C12M25/04—Membranes; Filters in combination with well or multiwell plates, i.e. culture inserts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- Bioinformatics & Cheminformatics (AREA)
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- General Health & Medical Sciences (AREA)
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- Virology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of pit microorganism in situ culture apparatus and method.Pit microorganism in situ culture apparatus, including cell body and pressing plate, it is removably installed on the cell body at the top of groove at least provided with a groove, pressing plate on cell body, groove is in communication with the outside at least provided with a through hole, through hole on cell body and/or pressing plate.Culture dish is positioned in groove, then fixed by pressing plate, pit microorganism in situ culture apparatus is placed directly in pit, through hole makes poor unstrained spirits be connected with culture dish, to carry out pit microorganism in situ culture experiment.For ease of operation, at least two sections of cell body connections can be formed into longer culture apparatus, the both ends of pit microorganism in situ culture apparatus set plug and handle respectively.Pit microorganism in situ culture apparatus and method are suitable for the Culture in situ experiment of aromatic Chinese spirit fermentation pit microorganism, and microorganism can be cultivated during pit spontaneous fermentation, and can independently determine incubation time and culture position, simple in construction and easy to operate.
Description
Technical field
The present invention relates to a kind of Culture in situ device and method suitable for aromatic Chinese spirit fermentation pit microorganism.
Background technology
It is well known that brewageing for aromatic Chinese spirit belongs to open-sky technique, composite bacteria fermentation.In brewed spirit process
In, a large amount of microorganisms enter fermentation system in environment, solid, liquid, gas boundary carry out complicated metabolism, energy conversion and
Event information transmission.By long-term domestication, microorganism has adapted to the extreme yeastings such as anoxic, hyperosmosis, low pH, is formed
Specific white wine microbial population, white wine microorganism interknit in fermentation system, interacted, and cooperate with ferment, antagonism altogether
Competition, decide the product quality of white wine.The research of white wine microorganism has to understanding brewed spirit mechanism, lifting white wine quality
Very important meaning.
At present, scientific research personnel generally uses microorganism pure culture technigne, and microorganism generally is limited into specific Nutrient medium
Cultivated under conditions of the parameter constants such as matter, temperature, pH, this artificial training mode is assisted jointly originally between often resulting in microorganism
The Natural Survival mode of work is collapsed, the extreme nutrition change in habitat, active material and the interruption of communication and ecological niche great change etc., is led
Cause microbial drug tolerance low.The limitation of pure culture technigne becomes the bottleneck of researching white spirit microbial diversity, and it is studied
As a result the present situation of white wine microbial diversity can not be reflected comprehensively so that the largely microbial resources quilt with very big application value
Bury.
In recent years, researcher recognizes some educable factors of restriction micro-organisms, and it is micro- to have explored some cultures
The new method of biology.2002, Kaeberlein etc. devised diffusion chamber cultivation, and the film of diffusion chamber can make environment composition in box
Interior to be swapped between environment, the clump count that can be turned out is higher than 300 times or so of control group, is also separated to two kinds and has previously been thought that
It is not educable microorganism.This method largely simulating natural environment, due to influence the growth of microorganism growth because
Son can pass freely through film, ensure that effect between microbiologic population and exchange, so as to improve the Culturability of microorganism.
2005, microorganism journal 05 phase in 2005《Primary Study of the near-nature forest pure culture method to Bacteria Culture》In one text, Ye Jiangyu
Etc. devising a kind of porose culture dish, in culture dish covered with do not allow bacterium by but nutriment can flow freely
Miillpore filter.The culture dish is put into separated Institute of Micro-biology during culture needs in habitat, is provided to be cultured bacterium with original
The possibility that raw environment is linked up so that the part bacterium of this ecological relationship of strong depend-ence is possible to be cultured out, overcomes biography
The defects of system pure culture is difficult to provide external source activity material.
At present, it there is no the device that Culture in situ is carried out to liquor fermentation pit microorganism.
The content of the invention
The technical problems to be solved by the invention are to provide a kind of pit microorganism in situ culture apparatus.
The technical solution adopted for the present invention to solve the technical problems is：Pit microorganism in situ culture apparatus, including groove
Body and pressing plate, the pressing plate is set at least provided with a groove, the top of groove on the cell body, the pressing plate is removably pacified
On the cell body at the top loaded on groove, at least provided with a through hole on the cell body and/or pressing plate, the through hole is by groove and outside
Boundary connects.
Culture dish is positioned in groove, then fixed by pressing plate, pit microorganism in situ culture apparatus is directly placed
In in pit, the through hole on cell body or pressing plate makes poor unstrained spirits be connected with culture dish, to carry out pit microorganism in situ culture experiment.
Specifically, the pressing plate is fixedly installed on the cell body of groove top by screw.
It is further that the both ends of the cell body set the attachment structure being adapted respectively.In order to different deep in pit
Degree carries out Culture in situ experiment, and above-mentioned at least two section pit microorganism in situ culture apparatuses are connected by attachment structure, formed
Longer pit microorganism in situ culture apparatus.
It is further that the through hole is only arranged on pressing plate, at least two grooves is set on cell body, and each groove is each other
Independent and along cell body length direction is arranged.
It is further that the pit microorganism in situ culture apparatus also includes plug, and one end of the plug is in circular cone
Or pyramid-shaped, the other end of plug set the attachment structure being adapted with the cell body either end.Plug is connected to cell body
One end, by plug can more easily by pit microorganism in situ culture apparatus insert pit in poor unstrained spirits in.
Further that the pit microorganism in situ culture apparatus also includes handle, the middle part of handle set with it is described
The attachment structure that cell body either end is adapted.By handle, it is easy to insert pit microorganism in situ culture apparatus in pit
Poor unstrained spirits in, and be also convenient for after culture terminates, effortlessly the poor unstrained spirits by pit microorganism in situ culture apparatus out of pit
Middle extraction.
Specifically, the attachment structure is the female adapter and male connection being adapted.
It is further that the pit microorganism in situ culture apparatus is made of stainless steel.
The present invention also provides a kind of by any one above-mentioned pit microorganism in situ culture apparatus progress pit microorganism
The method of Culture in situ：First pass through pressing plate culture dish is fixedly installed in groove, then by pit microorganism in situ culture apparatus
It is put into the interior carry out Culture in situ of pit.
Specifically, the height of the culture dish and the depth of groove are consistent, and the side wall of groove and bottom are without through hole, culture
The bottom of ware sets at least one hole and lays miillpore filter in the bottom of culture dish；Culture dish is positioned in groove, culture dish
Bottom be located at pressing plate side, then culture dish is fixedly installed in groove by pressing plate.
The beneficial effects of the invention are as follows：Pit microorganism in situ culture apparatus and method can be trained under the in situ environment of pit
Microorganism is supported, can independently determine incubation time and culture position, it is simple in construction, it is easy to operate.Be advantageous to further investigate white wine
Microorganism, reflects the diversity of pit microorganism comprehensively, and establishes microbial resources storehouse.
Brief description of the drawings
Fig. 1 is the structural representation of pit microorganism in situ culture apparatus of the present invention；
Fig. 2 is the side view of the culture apparatus of pit microorganism in situ shown in Fig. 1；
Fig. 3 is the structural representation of another embodiment of pit microorganism in situ culture apparatus of the present invention.
Parts, position and numbering in figure：Cell body 1, groove 2, pressing plate 3, screw 4, plug 5, handle 6.
Embodiment
The invention will be further described below in conjunction with the accompanying drawings.
As depicted in figs. 1 and 2, pit microorganism in situ culture apparatus of the present invention, including cell body 1 and pressing plate 3, the cell body
At least provided with a groove 2 on 1, three grooves 2 are set as shown；The top of groove 2 sets pressing plate 3, and pressing plate 3 is removably pacified
On the cell body 1 at the top loaded on groove 2, specifically, pressing plate 3 is fixedly installed in by screw 4 on the cell body 1 at the top of groove 2；Will
Culture dish is positioned in groove 2, then pressing plate 3 is fixedly mounted by screw 4, culture dish is fixed in groove 2.In order that
Culture dish connects with external world's holding, and groove 2 is in communication with the outside at least provided with a through hole, through hole on cell body 1 and/or pressing plate 3.
Through hole is set on pressing plate 3 as shown, and the side wall of groove 2 and bottom are without through hole.Through hole on pressing plate 3, which does not influence culture dish, to be fixed
In in groove 2, while retain the through hole of pressing plate 3 and have larger diameter, be easy to inside culture dish with fully carrying out thing with external environment condition
Matter, information interchange.
On cell body 1 set three cylindrical grooves 2, three grooves 2 be respectively arranged at the same side of cell body 1 and along
The length direction arrangement of cell body 1, the size dimension of three grooves 2 are consistent.The top of groove 2 sets pressing plate 3, and pressing plate 3 can be one
An individual overall or groove 2 corresponds to a pressing plate 3 respectively.Through hole is set on pressing plate 3, through hole is circular hole, circular hole it is straight
Footpath is less than the diameter of the cross section of groove 2.In order to ensure that pressing plate 3 can be with the culture dish in fastening grooves 2, while it is easy to individually remove
Each culture dish, each groove 2 are separately provided pressing plate 3, and four screws 4 are uniformly arranged around pressing plate 3.To avoid pressing plate 3 from hindering
Hinder the connection between outside inside culture dish, a circular hole is set on pressing plate 3.
Above-mentioned pit microorganism in situ culture apparatus is placed directly in the poor unstrained spirits in pit, you can carry out training in situ
Support.
The both ends of cell body 1 are shaft-like, and middle part is flat-shaped, and wherein the middle part of cell body 1 is the region for setting groove 2.For the ease of
Different depth, diverse location in pit carry out Culture in situ experiment, and the both ends of cell body 1 set the connection knot being adapted respectively
Structure.At least two section cell bodies 1 are joined end to end, form longer pit microorganism in situ culture apparatus.Specifically, the both ends of cell body 1
Attachment structure be respectively the female adapter and male connection that are adapted.
As shown in figure 3, for the ease of cell body 1 is inserted in the poor unstrained spirits in pit, pit microorganism in situ culture apparatus is also
Including plug 5, one end of plug 5 is in circular cone or pyramid-shaped, and the other end of plug 5 is the internal thread being adapted with the termination of cell body 1
Joint or male connection.Pit microorganism in situ culture apparatus also includes handle 6, and the middle part of handle 6 sets the end with cell body 1
The female adapter or male connection that head is adapted, and the joint of the joint of handle 6 and plug 5 differs.By handle 6,
It is easy to insert pit microorganism in situ culture apparatus in the poor unstrained spirits in pit, and is also convenient for after culture terminates, effortlessly
It will be extracted in poor unstrained spirits of the pit microorganism in situ culture apparatus out of pit.Three sections of cell bodies 1 are joined end to end respectively, reconnected
Plug 5 and handle 6, it is possible to which easily the arbitrary levels in pit upper, middle and lower-ranking grain unstrained spirits, any fermentation period progress are micro-
The Culture in situ operation of biology, it is possible to be greatly enhanced the Culturability of microorganism.
Whole pit microorganism in situ culture apparatus is made of stainless steel, i.e. cell body 1, pressing plate 3, screw 4, plug 5, hand
Handle 6 is made of stainless steel.
The present invention also provides a kind of by the progress pit microorganism in situ culture of above-mentioned pit microorganism in situ culture apparatus
Method, first pass through pressing plate 3 and culture dish be fixedly installed in groove 2, then pit microorganism in situ culture apparatus is put into cellar for storing things
Culture in situ is carried out in the poor unstrained spirits in pond.Wherein, the height of culture dish is consistent with the depth of groove 2, i.e., when culture dish covers adaptation
Culture dish lid after total height it is consistent with the depth of groove 2, with ensure can pressing plate 3 culture dish is carried out it is effectively fixed；It is recessed
Without through hole, the bottom of culture dish sets at least one hole, such as is set in the bottom even of culture dish for the side wall of groove 2 and bottom
5 to 6 circular holes, and lay miillpore filter in the bottom of culture dish；Culture dish is positioned in groove 2, and the bottom of culture dish is located at
Side where pressing plate 3, then culture dish is fixedly installed in groove 2 by pressing plate 3.
Pit microorganism in situ culture apparatus and method are suitable for the Culture in situ of aromatic Chinese spirit fermentation pit microorganism
Experiment, can cultivate microorganism during pit spontaneous fermentation, and can independently determine incubation time and culture position, structure
Simply, it is easy to operate.
Claims (8)
1. pit microorganism in situ culture apparatus, it is characterised in that：Including cell body (1) and pressing plate (3), cell body (1) both ends
To be shaft-like, to be flat-shaped, the both ends of cell body (1) set the attachment structure being adapted respectively at middle part, the middle part of cell body (1) at least provided with
One groove (2), the top of groove (2) set the pressing plate (3), and the pressing plate (3) is fixedly installed in groove by screw (4)
(2) on the cell body (1) at top, at least provided with a through hole on the cell body (1) and/or pressing plate (3), the through hole is by groove
(2) it is in communication with the outside.
2. pit microorganism in situ culture apparatus as claimed in claim 1, it is characterised in that：The through hole is only arranged at pressing plate
(3) on, at least two grooves (2) are set, each groove (2) is independently of one another and along the length direction of cell body (1) on cell body (1)
Arrangement.
3. pit microorganism in situ culture apparatus as claimed in claim 1, it is characterised in that：The pit microorganism in situ training
Foster device also includes plug (5), and one end of the plug (5) is in circular cone or pyramid-shaped, the other end of plug (5) set with it is described
The attachment structure that cell body (1) either end is adapted.
4. pit microorganism in situ culture apparatus as claimed in claim 1, it is characterised in that：The pit microorganism in situ training
Foster device also includes handle (6), and the middle part of handle (6) sets the attachment structure being adapted with the cell body (1) either end.
5. the pit microorganism in situ culture apparatus as described in claim 1 or 3 or 4 any claims, it is characterised in that：Institute
It is the female adapter and male connection being adapted to state attachment structure.
6. pit microorganism in situ culture apparatus as claimed in claim 1, it is characterised in that：The pit microorganism in situ training
Foster device is made of stainless steel.
7. the method for pit microorganism in situ culture, it is characterised in that：Pass through any claim of the claims 1 to 6 institute
The pit microorganism in situ culture apparatus stated carries out pit microorganism in situ culture：First pass through pressing plate (3) and fix culture dish and pacify
Loaded in groove (2), then pit microorganism in situ culture apparatus is put into the interior carry out Culture in situ of pit.
8. the method for pit microorganism in situ culture as claimed in claim 7, it is characterised in that：The height of the culture dish with
The depth of groove (2) is consistent, the side wall of groove (2) and bottom without through hole, the bottom of culture dish set at least one hole and
The bottom laying miillpore filter of culture dish；Culture dish is positioned in groove (2), and the bottom of culture dish is located at pressing plate side,
Culture dish is fixedly installed in groove (2) by pressing plate (3) again.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510931179.7A CN105368700B (en) | 2015-12-15 | 2015-12-15 | Pit microorganism in situ culture apparatus and method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510931179.7A CN105368700B (en) | 2015-12-15 | 2015-12-15 | Pit microorganism in situ culture apparatus and method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105368700A CN105368700A (en) | 2016-03-02 |
| CN105368700B true CN105368700B (en) | 2017-12-08 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510931179.7A Active CN105368700B (en) | 2015-12-15 | 2015-12-15 | Pit microorganism in situ culture apparatus and method |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5417576A (en) * | 1993-11-12 | 1995-05-23 | Iowa Methodist Medical Center | Means and method for microbiological growth and in situ observation with microscopes |
| CN104789449A (en) * | 2015-04-27 | 2015-07-22 | 国家***第一海洋研究所 | In-situ culturing device for deep-sea microbes |
| CN104807672A (en) * | 2015-05-08 | 2015-07-29 | 泸州品创科技有限公司 | Multi-point vinasse sampler and sampling method |
-
2015
- 2015-12-15 CN CN201510931179.7A patent/CN105368700B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5417576A (en) * | 1993-11-12 | 1995-05-23 | Iowa Methodist Medical Center | Means and method for microbiological growth and in situ observation with microscopes |
| CN104789449A (en) * | 2015-04-27 | 2015-07-22 | 国家***第一海洋研究所 | In-situ culturing device for deep-sea microbes |
| CN104807672A (en) * | 2015-05-08 | 2015-07-29 | 泸州品创科技有限公司 | Multi-point vinasse sampler and sampling method |
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| Publication number | Publication date |
|---|---|
| CN105368700A (en) | 2016-03-02 |
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