CN105367250B - A kind of compound preparation improving Dendrobidium huoshanness alkaloid - Google Patents
A kind of compound preparation improving Dendrobidium huoshanness alkaloid Download PDFInfo
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- CN105367250B CN105367250B CN201510839195.3A CN201510839195A CN105367250B CN 105367250 B CN105367250 B CN 105367250B CN 201510839195 A CN201510839195 A CN 201510839195A CN 105367250 B CN105367250 B CN 105367250B
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Abstract
The invention discloses a kind of compound preparations for improving Dendrobidium huoshanness alkaloid, said preparation comprises the following raw materials by weight percent: ethephon (CEPHA),2-(chloroethyl) phosphonic acid 0.02 ~ 0.04%, jasmonic formicester 0.01 ~ 0.03%, tryptophan 0.002 ~ 0.005%, lysine 0.001 ~ 0.003%, citric acid 0.001 ~ 0.003%, succinylbenzoic acid 0.002 ~ 0.004%, potassium dihydrogen phosphate 0.005 ~ 0.008%, organosilicon auxiliary agent 0.1 ~ 0.3%, surplus are water.Annual dendrobium nobile harvests first 20 ~ 30 days, is taken shelter from rain with above-mentioned compound preparation and sprays Dendrobidium huoshanness seedling plant, and fountain height is 20 ~ 40 mL/m2, spray 1 time within 5 ~ 7 days, co-continuous sprinkling 2 ~ 4 times.Using compound preparation provided by the invention, the content of Dendrobidium huoshanness total alkaloid can be significantly improved.
Description
Technical field
The present invention relates to a kind of plant growth regulator technology, it is specifically related to a kind of Dendrobidium huoshanness alkaloid that improves
Compound preparation.
Background technique
Dendrobium nobile (Dendrobium) is the rare rare medicinal material in China, and nature and flavor are sweet, slightly cold, enters lung, stomach, kidney three and passes through, promotes the production of body fluid
Beneficial stomach, clearing heat and nourishing yin, moistening lung to arrest cough, tonifying kidney and improving eyesight.There are three types of is produced from Huoshan County of Anhui Province: Dendrobidium huoshanness, Dendrobium Moniliforme and iron sheet
Dendrobium nobile, wherein Dendrobidium huoshanness is the top grade in Chinese medicine dendrobium nobile.
Dendrobidium huoshanness (Dendrobium huoshanense), is commonly called as a meter dry measure used in former times, is orchid family Dendrobium herbaceos perennial,
Limitation be distributed in the counties such as Huoshan, Jinzhai County, Yuexi, Shucheng County and the Hubei Yingshan Mountains of Da Bie Mountain area, containing polysaccharide, alkaloid and
Flavones isoreactivity ingredient has enhancing immunocompetence, improving eyesight, anti-oxidant and other effects, is one of Anhui genunie medicinal materials.Chinese medicine dendrobium nobile
Main effective ingredient be the alkaloids such as dendrobine, nobilonine and dendramine, with it is important it is anti-oxidant, remove free radical
Function.Dendrobium nobile alkaloid have a variety of configurations, as sesquiterpenoids, octahydro indolizine class, nafoxidine class, indoles connection pyridine class and
Imidazoles etc..Studies have shown that dendrobium nobile alkaloid has the function of anti-sugar cataract, significant raising crystalline lens water-solubility protein,
Reduced glutathione content and superoxide dismutase activity reduce mda content；In addition, alkaloid also has immune tune
Section, anti-inflammatory, expansion blood vessel and blood coagulation resisting function etc., improve the function of hypomnesia.
However, one of the active constituent that alkaloid is important as Dendrobidium huoshanness, but very low in the intracorporal content of dendrobium nobile.According to
Fourth Asia equality people is the study found that alkaloid is the 0.029% of dry weight, only dendrobium stem in the Dendrobidium huoshanness of triennial
/ 20th or so of alkali content.Therefore, the content for improving Dendrobidium huoshanness alkaloid is both to improve Dendrobidium huoshanness medical value
Important channel, and a large amount of necessity for extracting Huoshan alkaloid activity ingredient has fabulous economic and social profit.
The study found that the synthesis of most of alkaloids is all to be related to series of intermediate products on the way from amino acid
And enzyme.In addition, the natural synthesis of alkaloid belongs to the scope of Secondary Metabolism of Plant, by the regulation of many factors, such as temperature height
Low, illumination is strong and weak and hormonal readiness etc..A large number of studies show that increasing the precursor substance of secondary metabolite, generation can be promoted
Thank to the accumulation of product.For example, application exogenous tryptophan, can promote the synthesis of indoles alkaloid；Research is it is also shown that plant swashs
Element, such as ethylene, methyl jasmonate, can regulate and control the synthesis of Secondary metabolites.For example, passing through regulation Asian puccoon endogenous ethylene
Synthesis, anti-tumor active ingredient can be regulated and controled --- the synthesis of shikonin.
Currently, there is not been reported about the research for improving Dendrobidium huoshanness alkaloid.The invention patent is raw according to dendrobium nobile
The structure feature and Secondary Metabolism of Plant regulatory mechanism of alkaloids, using the precursor small-molecule substance and external source of addition alkaloid synthesis
The mode of hormone, the preparation and Rational Application of preparation regulation dendrobium nobile alkaloid synthesis, to improve the content of alkaloid, to improve
The medical value of Dendrobidium huoshanness.
Summary of the invention
The purpose of the invention patent is to improve Dendrobidium huoshanness alkaloid and providing a compound preparation, using of the invention
Method can significantly improve the content of Dendrobidium huoshanness alkaloid, improve its medical value, and without expensive early investment, behaviour
Make easy.
The invention patent is achieved through the following technical solutions:
(1) a kind of compound preparation for improving Dendrobidium huoshanness alkaloid, comprises the following raw materials by weight percent: second
Alkene benefit 0.02 ~ 0.04%, jasmonic formicester 0.01 ~ 0.03%, tryptophan 0.002 ~ 0.005%, lysine 0.001 ~ 0.003%, lemon
Lemon acid 0.001 ~ 0.003%, succinylbenzoic acid 0.002 ~ 0.004%, potassium dihydrogen phosphate 0.005 ~ 0.008%, organosilicon auxiliary agent
0.1 ~ 0.3%, surplus is water.It is used after sufficiently dissolving and mix；(2) a kind of raising Dendrobidium huoshanness alkaloid described in
Compound preparation, can be ready-to-use, can also room temperature be kept in dark place 30 days or 4 DEG C save 1 year；(3) a kind of raising Huoshan stone described in
The compound preparation of dry measure used in former times alkaloid, method of administration are as follows: annual dendrobium nobile harvests first 20 ~ 30 days, is kept away with above-mentioned compound preparation
Rain sprays Dendrobidium huoshanness seedling plant, and fountain height is 20 ~ 40 mL/m2, spray 1 time within 5 ~ 7 days, co-continuous sprinkling 2 ~ 4 times；(4) this hair
A kind of compound preparation of bright raising Dendrobidium huoshanness alkaloid, is not limited to the production of Dendrobidium huoshanness, further includes various
The production of other dendrobium species.
Beneficial effects of the present invention:
Using a kind of compound preparation for improving Dendrobidium huoshanness alkaloid provided by the invention, Huoshan stone can be significantly improved
The content of dry measure used in former times total alkaloid, average content 0.182%, the Dendrobidium huoshanness seedling alkaloid than not spraying the compound preparation mention
It is 5.28 times high.Table is first is that influence of the sprinkling compound preparation of the present invention to Dendrobidium huoshanness alkaloid, the results showed that sprinkling is originally
The compound preparation that invention provides can significantly improve Dendrobidium huoshanness alkaloid.
Table one sprays influence of the compound preparation of the present invention to Dendrobidium huoshanness alkaloid.
Note: * * indicates that difference is extremely significant (P < 0.01, LSD).
Specific embodiment
Method therefor is conventional method unless otherwise instructed in following embodiments.It should be appreciated that described herein excellent
It selects embodiment to be merely to illustrate and explain the present invention, but is not intended to limit the present invention.
Embodiment 1:
Each raw material: ethephon (CEPHA),2-(chloroethyl) phosphonic acid 0.2g, methyl jasmonate 0.1g, tryptophan 0.02g, lysine are weighed according to following weight
0.01g, citric acid 0.01g, succinylbenzoic acid 0.02g, potassium dihydrogen phosphate 0.05g, organosilicon auxiliary agent 1.5g are dissolved in water and determine
Hold to 1000g(and be approximately equivalent to 1000mL) form compound preparation.It takes shelter from rain within first 20 days in harvesting and sprays above-mentioned compound preparation, every square
Rice Dendrobidium huoshanness seedling sprays 20mL, continues sprinkling after 5 days once, sprays 2 times altogether.Huoshan stone is processed into after harvesting according to a conventional method
Dry measure used in former times dry product weighs after 10g dry product is clayed into power and measures its total alkaloid content.
Embodiment 2:
Each raw material: ethephon (CEPHA),2-(chloroethyl) phosphonic acid 0.3g, methyl jasmonate 0.15g, tryptophan 0.03g, lysine are weighed according to following weight
0.018g, citric acid 0.015g, succinylbenzoic acid 0.04g, potassium dihydrogen phosphate 0.06g, organosilicon auxiliary agent 1g are dissolved in water and determine
Hold to 1000g(and be approximately equivalent to 1000mL) form compound preparation.It takes shelter from rain within first 26 days in harvesting and sprays above-mentioned compound preparation, every square
Rice Dendrobidium huoshanness seedling sprays 35mL, continues sprinkling after 7 days once, sprays 3 times altogether.Huoshan stone is processed into after harvesting according to a conventional method
Dry measure used in former times dry product weighs after 10g dry product is clayed into power and measures its total alkaloid content.
Embodiment 3:
Each raw material: ethephon (CEPHA),2-(chloroethyl) phosphonic acid 0.4g, methyl jasmonate 0.25g, tryptophan 0.04g, lysine are weighed according to following weight
0.02g, citric acid 0.018g, succinylbenzoic acid 0.03g, potassium dihydrogen phosphate 0.08g, organosilicon auxiliary agent 2g are dissolved in water and determine
Hold to 1000g(and be approximately equivalent to 1000mL) form compound preparation.It takes shelter from rain within first 30 days in harvesting and sprays above-mentioned compound preparation, every square
Rice Dendrobidium huoshanness seedling sprays 30mL, continues sprinkling after 6 days once, sprays 4 times altogether.
Claims (4)
1. a kind of compound preparation for improving Dendrobidium huoshanness alkaloid, which is characterized in that by the raw material of following weight percents
Composition: ethephon (CEPHA),2-(chloroethyl) phosphonic acid 0.02~0.04%, methyl jasmonate 0.01~0.03%, tryptophan 0.002~0.005%, lysine
0.001~0.003%, citric acid 0.001~0.003%, succinylbenzoic acid 0.002~0.004%, potassium dihydrogen phosphate
0.005~0.008%, organosilicon auxiliary agent 0.1~0.3%, surplus is water.
2. according to described in claim 1, which is characterized in that be made of the raw material of following weight percents: ethephon (CEPHA),2-(chloroethyl) phosphonic acid 0.03%, jasmine
Jasmine acid methyl esters 0.015%, tryptophan 0.003%, lysine 0.0018%, citric acid 0.0015%, succinylbenzoic acid
0.004%, potassium dihydrogen phosphate 0.006%, organosilicon auxiliary agent 0.1%, surplus is water.
3. the application of compound preparation described in accordance with the claim 1, which is characterized in that annual dendrobium nobile harvests first 20~30 days, uses
Above-mentioned compound preparation, which is taken shelter from rain, sprays Dendrobidium huoshanness seedling plant, and fountain height is 20~40mL/m2, spray 1 time within 5~7 days, co-continuous spray
It spills 2~4 times.
4. the application of compound preparation described in accordance with the claim 3, which is characterized in that the compound preparation is not limited to Dendrobidium huoshanness
Production, further include the production of various other dendrobium species.
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| CN107382539A (en) * | 2017-09-06 | 2017-11-24 | 汪建利 | Improve air ambient and promote biological agent of photosynthesis of plant and preparation method thereof |
| CN109105037B (en) * | 2018-10-11 | 2021-01-08 | 中国科学院昆明植物研究所 | Method for producing scopoletin by inducing tobacco |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1799335A (en) * | 2005-12-19 | 2006-07-12 | 东北林业大学 | Method for improving vinblastine content in madagascar periwinkle |
| WO2009066818A1 (en) * | 2007-11-20 | 2009-05-28 | Korea Research Institute Of Bioscience And Biotechnology | Composition and method for increasing resistance against plant pathogen by comprising bacterial genetic materials, and plant produced by the method |
| CN103109688A (en) * | 2013-02-06 | 2013-05-22 | 淮北师范大学 | Method for delaying sprout tumbling at high temperature and raising yield and alkaloid content of pinellia ternate |
| CN104186223A (en) * | 2014-08-15 | 2014-12-10 | 上海交通大学 | Method for disposing and improving alkaloid content in catharanthus roseus through plant growth regulators |
| CN104591813A (en) * | 2015-02-10 | 2015-05-06 | 湛社铭 | Organic amino acid fertilizer for rice yield increase and application method thereof |
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2015
- 2015-11-27 CN CN201510839195.3A patent/CN105367250B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1799335A (en) * | 2005-12-19 | 2006-07-12 | 东北林业大学 | Method for improving vinblastine content in madagascar periwinkle |
| WO2009066818A1 (en) * | 2007-11-20 | 2009-05-28 | Korea Research Institute Of Bioscience And Biotechnology | Composition and method for increasing resistance against plant pathogen by comprising bacterial genetic materials, and plant produced by the method |
| CN103109688A (en) * | 2013-02-06 | 2013-05-22 | 淮北师范大学 | Method for delaying sprout tumbling at high temperature and raising yield and alkaloid content of pinellia ternate |
| CN104186223A (en) * | 2014-08-15 | 2014-12-10 | 上海交通大学 | Method for disposing and improving alkaloid content in catharanthus roseus through plant growth regulators |
| CN104591813A (en) * | 2015-02-10 | 2015-05-06 | 湛社铭 | Organic amino acid fertilizer for rice yield increase and application method thereof |
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