CN105359969A - Fast propagation method of moringa stem segments - Google Patents
Fast propagation method of moringa stem segments Download PDFInfo
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- CN105359969A CN105359969A CN201410448829.8A CN201410448829A CN105359969A CN 105359969 A CN105359969 A CN 105359969A CN 201410448829 A CN201410448829 A CN 201410448829A CN 105359969 A CN105359969 A CN 105359969A
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Abstract
The invention provides a fast propagation method of moringa stem segments. Stems are acquired from moringa plants, the moringa stems are inoculated to an induction culture medium after being disinfected, then the induction culture medium is transferred to a rooting culture medium, and therefore whole plants can be obtained; tender buds growing through induction can also be divided into many stem segments, and the purpose of fast propagation is achieved through repeated culture. The plants obtained through the method are small in progeny variation, and support is provided for fast development of the moringa industry.
Description
Technical field
The present invention relates to biological self reproducing technical field, be specifically related to a kind of method of Moringa stem section Fast-propagation.
Background technology
Moringa tree (Classification system: MoringatreeOleiferaLamarch, English name: Moringatree), also known as drumstick tree, horseradish tree, belong to Moringa tree section, for perennial evergreen dungarunga is to megaphanerophyte, existing knownly have 14 kinds, edible cultivation have sajina to set and African Moringa sets two kinds of kinds.Originate in the torrid zone, the arid of south subtropics or semiarid zone, have very strong adaptability to soil condition and rainfall, strong stress resistance.Moringa complete stool all can utilize, and nutriment kind is many.Moringa blade, flower, fruit pod are rich in protein, various vitamin and mineral nutrient element, and it is reported that wherein protein content is 2 times of milk, Vc is 7 times of oranges and tangerines, V
abe 4 times of carrot, calcium content is 4 times of milk, and potassium is 3 times of banana, and iron is 3 times of spinach, and each seed amino acid in addition containing needed by human and trace element etc., have augment nutritional and Dietotherapy health function as vegetables and food; Moringa seed contains active coagulated components, there is the specific function of purifying waste water, therefore be widely used in the multiple fields such as agricultural, industry, livestock breeding, medicine, beauty treatment, be a kind of rare dish integrating delicious food, nutrition and health care, be described as " tree of miracle ".
The method of domestic breeding Moringa mainly planting seed at present, this method is subject to the restriction in season, and germination rate and reproduction coefficient lower, variation is large, is difficult to carry out scale, standardized production, is difficult to the spread carrying out improved seeds.
Summary of the invention
The invention provides one efficiently, Moringa seedling breeding method fast.Moringa stem section culture refers to clip stem segment with axillary bud from Moringa plant, is seeded on axillary bud deriving medium after sterilization, can induction buds sprouting; Through inducing the tender shoots grown can be divided into again many stem sections, again induce into sprouting.Namely form a complete plant after each sprouting sends out roots on root media, can field be transplanted to.So repeatedly induce, thus reach the object of rapid expansion breeding.Adopt the mode of axillary bud deriving to carry out numerous soon, occur that the probability of variation is extremely low, therefore also can ensure the genetic stability of offspring by Fast-propagation excellent strain in a large number.This technology not only can obtain a large amount of Moringa seedling in the short time, is also the effective means that elite germplasm is preserved, bred and promote.
The present invention adopts following technical scheme for achieving the above object:
A method for Moringa stem section Fast-propagation, is characterized in that: specifically comprise the following steps:
(1) from the stem section of clip band axillalry bud Moringa wood plant;
(2) the stem section of the band axillalry bud of clip is carried out disinfection;
(3) stem segment with axillary bud after sterilization is seeded in Fiber differentiation on axillary bud deriving medium, cultivates and grow tender shoots in about 30 days;
(4) by inducing the tender shoots grown to be divided into many stem sections again, the operation of step (3) is then repeated;
(5) when bud growing way is comparatively strong, transfer them in root media, wait after sending out roots, then be transplanted to field, so repeat to cultivate, thus reach the object of Fast-propagation.
The method of described Moringa stem section Fast-propagation, it is characterized in that: described disinfects, concrete operations are: first in the ethanolic solution of 75%, soak 0.5min-1.0min, and then soak 5min-8min with the HgCl2 solution of 0.2%, and period ceaselessly shakes.
The method of described Moringa stem section Fast-propagation, is characterized in that: described bud inducement medium refers to: MS medium+6-BA0.3mg/L+NAA0.1mg/L.
The method of described Moringa stem section Fast-propagation, is characterized in that: described root media refers to: 1/2MS medium+6-BA0.9mg/L+NAA0.1mg/L+ carbon dust 1g/L.
Beneficial effect of the present invention:
The inventive method solves the low problem of Moringa seed reproductive efficiency, and not by the restriction of season, time, the progeny of plants obtained by this inventive method variation is little, for the fast development of Moringa provides support.
Embodiment
First get Moringa plants stems section, immersion treatment 0.5min in the ethanol of 75%, use the HgCl of 0.2% afterwards again
2
Immersion treatment 8min, period ceaselessly shakes.After disinfecting, be cut into about about 1cm's with aseptic scalpel
Stem segment with axillary bud, is seeded on bud inducement medium, about about 20d afterwards, and can induce the tender shoots that 4cm-8cm is long, the tender shoots grown can be divided into again many stem sections, again induces into sprouting, and its inductivity reaches more than 80%; When bud growing way is comparatively strong, transfer them in root media, about 10d can see that the tip of a root grows, and after 10d, average every strain can grow 2-3 bar
Healthy and strong root system, then have the whole plant of root and sprouting to be transplanted to field after hardening by long.
Claims (4)
1. a method for Moringa stem section Fast-propagation, is characterized in that: specifically comprise the following steps:
(1) from the stem section of clip band axillalry bud Moringa plant;
(2) the stem section of the band axillalry bud of clip is carried out disinfection;
(3) stem segment with axillary bud after sterilization is seeded in Fiber differentiation on axillary bud deriving medium, cultivates about 20d long
Go out tender shoots;
(4) by inducing the tender shoots grown to be divided into many stem sections again, the operation of step (3) is then repeated;
(5) when bud growing way is comparatively strong, transfer them in root media, wait after sending out roots, then be transplanted to field after hardening, so repeat to cultivate, thus reach the object of Fast-propagation.
2. the method for Moringa stem section Fast-propagation according to claim 1, it is characterized in that: described disinfects, concrete operations are: first in the ethanolic solution of 75%, soak 0.5min-1.0min, and then with 0.2% HgCl
2solution soaks 5min-8min, and period ceaselessly shakes.
3. the method for Moringa stem section Fast-propagation according to claim 1, is characterized in that: described bud inducement medium refers to: MS medium+6-BA0.3mg/L+NAA0.1mg/L.
4. the method for Moringa stem section Fast-propagation according to claim 1, is characterized in that: described root media refers to: 1/2MS medium+6-BA0.9mg/L+NAA0.1mg/L+ carbon dust 1g/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410448829.8A CN105359969A (en) | 2014-08-27 | 2014-08-27 | Fast propagation method of moringa stem segments |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410448829.8A CN105359969A (en) | 2014-08-27 | 2014-08-27 | Fast propagation method of moringa stem segments |
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| CN105359969A true CN105359969A (en) | 2016-03-02 |
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| CN201410448829.8A Pending CN105359969A (en) | 2014-08-27 | 2014-08-27 | Fast propagation method of moringa stem segments |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105961197A (en) * | 2016-05-09 | 2016-09-28 | 华南农业大学 | High efficiency regeneration method of Moringa oleifera |
| CN106718941A (en) * | 2017-02-15 | 2017-05-31 | 钦州市林业科学研究所 | A kind of construction method of Moringa regenerating system |
| CN108040535A (en) * | 2018-01-19 | 2018-05-18 | 云南省德宏热带农业科学研究所 | A kind of Moringa green cultivation management method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102150619A (en) * | 2011-03-09 | 2011-08-17 | 云南省农业科学院花卉研究所 | Moringa embryo callus induction and plant regeneration method |
-
2014
- 2014-08-27 CN CN201410448829.8A patent/CN105359969A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102150619A (en) * | 2011-03-09 | 2011-08-17 | 云南省农业科学院花卉研究所 | Moringa embryo callus induction and plant regeneration method |
Non-Patent Citations (2)
| Title |
|---|
| 武新琴等: "不同激素配比对辣木组培苗生长的影响", 《山西农业大学》 * |
| 黎国运等: "辣木组培育苗技术研究总结", 《林业科技》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105961197A (en) * | 2016-05-09 | 2016-09-28 | 华南农业大学 | High efficiency regeneration method of Moringa oleifera |
| CN106718941A (en) * | 2017-02-15 | 2017-05-31 | 钦州市林业科学研究所 | A kind of construction method of Moringa regenerating system |
| CN106718941B (en) * | 2017-02-15 | 2019-04-19 | 钦州市林业科学研究所 | A kind of construction method of Moringa regenerating system |
| CN108040535A (en) * | 2018-01-19 | 2018-05-18 | 云南省德宏热带农业科学研究所 | A kind of Moringa green cultivation management method |
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Application publication date: 20160302 |
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