CN105349342A - Method for preparing nutritional wine by using composition of fungi, algae and dendrobium - Google Patents

Method for preparing nutritional wine by using composition of fungi, algae and dendrobium Download PDF

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Publication number
CN105349342A
CN105349342A CN201510944440.7A CN201510944440A CN105349342A CN 105349342 A CN105349342 A CN 105349342A CN 201510944440 A CN201510944440 A CN 201510944440A CN 105349342 A CN105349342 A CN 105349342A
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China
Prior art keywords
stem
algae
noble dendrobium
composition
parts
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Chinese (zh)
Inventor
庄济诚
李江波
钱丽华
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SUZHOU YUANMENG NEW ENERGY ENVIRONMENTAL PROTECTION TECHNOLOGY Co Ltd
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SUZHOU YUANMENG NEW ENERGY ENVIRONMENTAL PROTECTION TECHNOLOGY Co Ltd
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Priority to CN201510944440.7A priority Critical patent/CN105349342A/en
Publication of CN105349342A publication Critical patent/CN105349342A/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12G—WINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
    • C12G3/00—Preparation of other alcoholic beverages
    • C12G3/02—Preparation of other alcoholic beverages by fermentation
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/06—Fungi, e.g. yeasts
    • A61K36/07—Basidiomycota, e.g. Cryptococcus
    • A61K36/076—Poria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/484—Glycyrrhiza (licorice)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/72—Rhamnaceae (Buckthorn family), e.g. buckthorn, chewstick or umbrella-tree
    • A61K36/725—Ziziphus, e.g. jujube
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/81—Solanaceae (Potato family), e.g. tobacco, nightshade, tomato, belladonna, capsicum or jimsonweed
    • A61K36/815—Lycium (desert-thorn)

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  • Health & Medical Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Botany (AREA)
  • Biotechnology (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Medical Informatics (AREA)
  • Veterinary Medicine (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The invention provides a method for preparing a nutritional wine by using a composition of fungi, algae and dendrobium. The method comprises the following specific steps: 1) mixing dendrobium, spirulina, a yeast culture medium and saccharification powder in proportion, adding water of which the weight is 5 times that of the nutrient substances, adding an enzyme, adjusting the pH value, setting the temperature between 25 and 35 DEG C, performing enzymolysis for 28-36h, then centrifuging at a speed of 4000r/30min, and taking supernatant fluid for later use; 2) levigating a traditional Chinese medicine composition, sieving through a 30-mesh sieve to obtain 15 parts of mixed powder, mixing 25 parts of white sugar, 105g of fructose and 350ml of pure water to obtain saccharification liquid, adding 0.5ml of yeast cultured on a potato culture medium in advance, and fermenting for 4-6 days at 15-25 DEG C to obtain fermentation broth; 3) preparing a breeding yeast culture medium formula containing 30% of potatoes, 1.5% of glucose, 0.5% of peptone, 0.1% of magnesium sulfate, 0.1% of calcium chloride and 0.05% of KH2PO4, and performing inoculation under an aseptic condition after cooling at a certain pressure; and 4) adding 2.6L of mixed supernatant fluid obtained after enzymolysis into the fermentation broth, fermenting at 15-25 DEG C, and then performing deactivation, filtration, standing and flavoring to obtain a finished product.

Description

A kind of method preparing nutriment wine with bacterium, algae, stem of noble dendrobium composition
Technical field
The present invention relates to a kind of method preparing nutriment wine with bacterium, algae, stem of noble dendrobium composition, dependent territory drinking production technical field.
Background technology
Air in recent years, water source soil pollution is day by day serious, adverse weather; The temperature difference, sleet is changeable, and impel the variations such as pathogeny bacterium to strengthen, burst disease (SARS, bird flu etc.), makes human body decline to the adaptive faculty of environment, is in sub-health state; In addition illegally make and sell interpolation toxic substance of poor quality without good businessman, impel people's resist the disease ability (immunizing power) to decline, accelerate disease (cancer) and development and old and feeble process occur.For this reason, we, while striving for clean air and water source, must strengthen autoprotection; Except appropriate exercise, harmless food products and trophic function food, become people preferred.
The application of nutritive food, pays close attention to both at home and abroad already, especially in recent years to carry out clinical nutrition, more attracts people's attention, and the medical institutions of China various places are all provided with and face product bed Nutriology Dept., are carried out by medical practitioner.
Summary of the invention
A kind of method preparing nutriment wine with bacterium, algae, stem of noble dendrobium composition of the present invention, concrete steps are:
1) stem of noble dendrobium, spirulina, yeast culture medium and saccharification powder are mixed in proportion, add 5 times to the water of above-listed nutrition weight, and add enzyme, regulate pH, between temperature setting 25 ~ 35 DEG C, after enzymolysis 28 ~ 36h, be placed in 4000r/30min centrifugal, get supernatant liquor, for subsequent use;
2) lead Chinese medicine composition levigate, and cross 30 mesh sieves, obtain powder mix 15 parts, white sugar 25 parts, fructose 10 5g, water purification 350ml is mixed into saccharified liquid, adds the yeast 0.5ml cultivated on potato culture in advance, 15 ~ 25 DEG C of indirect fermentations 4 ~ 6 days, obtains fermented liquid;
3) selecting yeast culture medium prescription: potato 30%, glucose 1.5%, peptone 0.5%, sulphur
Acid magnesium 0.1%, calcium chloride 0.1%, KH 2pO 40.05%, aseptic condition after cooling under a certain pressure
Lower inoculation;
4) fermented liquid adds the mixing supernatant 2.6L after enzymolysis, kill to live → filter after 15 ~ 25 DEG C of indirect fermentations → leave standstill → seasoning → finished product.
The weight part of described each material is: the stem of noble dendrobium 20 ~ 35 parts, spirulina 20 ~ 40 parts, Chinese medicine composition 5 ~ 10 parts, yeast culture medium 10 ~ 25 parts, enzyme 5 ~ 15 parts, 8 ~ 15 parts, saccharification powder.
Described Chinese medicine composition is the mixture of Radix Glycyrrhizae, matrimony vine, red date, Poria cocos.
Described enzyme is hemicellulase, cellulase, saccharifying enzyme and proteolytic enzyme.
Preferably, described step 1) middle pH=5.0 ~ 7.8.
Preferably, described step 1) in temperature be 30 DEG C.
Preferably, described step 1) in enzymolysis time be 32h.
Preferably, described step 3) in pressure be 1.176 × 10 5pa.
Preferably, described step 4) in fermentation time be 2 ~ 5 days.
Beneficial effect of the present invention is: scheme is complete; Operation is simple, easy handling; Cost of manufacture is cheap; Its product can effective accelerating wound healing.
Embodiment
Embodiment 1:
1) stem of noble dendrobium, spirulina, yeast culture medium and saccharification powder are mixed in proportion, add 5 times to the water of above-listed nutrition weight, and add hemicellulase, cellulase, saccharifying enzyme and proteolytic enzyme, pH is regulated to be 5.0, temperature sets between 25 DEG C, after enzymolysis 28h, is placed in 4000r/30min centrifugal, get supernatant liquor, for subsequent use; 2) lead Chinese medicine composition levigate, and cross 30 mesh sieves, obtain powder mix 15 parts, white sugar 25 parts, fructose 10 5g, water purification 350ml is mixed into saccharified liquid, adds the yeast 0.5ml cultivated on potato culture in advance, 15 DEG C of indirect fermentations 4 days, obtains fermented liquid; 3) selecting yeast culture medium prescription: potato 30%, glucose 1.5%, peptone 0.5%, magnesium sulfate 0.1%, calcium chloride 0.1%, KH 2pO 40.05%, 1.176 × 10 5inoculate under aseptic condition after cooling under Pa; 4) fermented liquid adds the mixing supernatant 2.6L after enzymolysis, kill after 2 days at 15 ~ 25 DEG C of indirect fermentations to live → filter → leave standstill → seasoning → finished product.
The weight part of described each material is: stem of noble dendrobium 20kg, spirulina 20kg, Chinese medicine composition 5kg, yeast culture medium 10kg, enzyme 5kg, saccharification powder 8kg.
Wherein, Chinese medicine composition is Radix Glycyrrhizae 1kg, matrimony vine 1kg, red date 1kg, Poria cocos 2kg.
Embodiment 2:
1) stem of noble dendrobium, spirulina, yeast culture medium and saccharification powder are mixed in proportion, add 5 times to the water of above-listed nutrition weight, and add hemicellulase, cellulase, saccharifying enzyme and proteolytic enzyme, pH is regulated to be 7.0, between temperature setting 25 ~ 35 DEG C, after enzymolysis 32h, be placed in 4000r/30min centrifugal, get supernatant liquor, for subsequent use; 2) lead Chinese medicine composition levigate, and cross 30 mesh sieves, obtain powder mix 15 parts, white sugar 25 parts, fructose 10 5g, water purification 350ml is mixed into saccharified liquid, adds the yeast 0.5ml cultivated on potato culture in advance, 20 DEG C of indirect fermentations 5 days, obtains fermented liquid; 3) selecting yeast culture medium prescription: potato 30%, glucose 1.5%, peptone 0.5%, magnesium sulfate 0.1%, calcium chloride 0.1%, KH 2pO 40.05%, 1.176 × 10 5inoculate under aseptic condition after cooling under Pa; 4) fermented liquid adds the mixing supernatant 2.6L after enzymolysis, kill after 3 days at 20 DEG C of indirect fermentations to live → filter → leave standstill → seasoning → finished product.
The weight part of described each material is: stem of noble dendrobium 25kg, spirulina 30kg, Chinese medicine composition 8kg, yeast culture medium 15kg, enzyme 10kg, saccharification powder 10kg.
Wherein, Chinese medicine composition is Radix Glycyrrhizae 1kg, matrimony vine 1kg, red date 1kg, Poria cocos 2kg.
Embodiment 3:
1) stem of noble dendrobium, spirulina, yeast culture medium and saccharification powder are mixed in proportion, add 5 times to the water of above-listed nutrition weight, and add hemicellulase, cellulase, saccharifying enzyme and proteolytic enzyme, pH is regulated to be 7.8, temperature sets between 35 DEG C, after enzymolysis 36h, is placed in 4000r/30min centrifugal, get supernatant liquor, for subsequent use; 2) lead Chinese medicine composition levigate, and cross 30 mesh sieves, obtain powder mix 15 parts, white sugar 25 parts, fructose 10 5g, water purification 350ml is mixed into saccharified liquid, adds the yeast 0.5ml cultivated on potato culture in advance, 25 DEG C of indirect fermentations 6 days, obtains fermented liquid; 3) selecting yeast culture medium prescription: potato 30%, glucose 1.5%, peptone 0.5%, magnesium sulfate 0.1%, calcium chloride 0.1%, KH 2pO 40.05%, 1.176 × 10 5inoculate under aseptic condition after cooling under Pa; 4) fermented liquid adds the mixing supernatant 2.6L after enzymolysis, kill after 5 days at 25 DEG C of indirect fermentations to live → filter → leave standstill → seasoning → finished product.
The weight part of described each material is: stem of noble dendrobium 35kg, spirulina 40kg, Chinese medicine composition 10kg, yeast culture medium 25kg, enzyme 15kg, saccharification powder 15kg.
Wherein, Chinese medicine composition is Radix Glycyrrhizae 1kg, matrimony vine 1kg, red date 1kg, Poria cocos 2kg.
Immune function experiment:
Get body weight 20 ~ 25g small white mouse 20, be divided into two groups at random: first group is filled with and fed water and food (comparing); Second group fills with the water and food of feeding quantity same with first group, increases 6ml nutriment wine (every day 2 times, each 3ml), detects respectively after 20 days:
Mouse leg belly is burnt with spirit lamp, the total area about 35%, the degree of depth is 5% II+wound, and with nutriment wine, be coated with abrasion, observe healing state suitable for reading and detect hemolysin, the items such as lymphocyte transformation, it is very fast that result fills with its wound healing of mouse of feeding nutriment wine, and hemolysin and lymphocyte etc. all have improvement in various degree.
More than show and describe ultimate principle of the present invention and principal character and advantage of the present invention.The technician of the industry should understand; the present invention is not restricted to the described embodiments; what describe in above-described embodiment and specification sheets just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.Application claims protection domain is defined by appending claims and equivalent thereof.

Claims (9)

1. prepare a method for nutriment wine with bacterium, algae, stem of noble dendrobium composition, it is characterized in that, concrete steps are:
1) stem of noble dendrobium, spirulina, yeast culture medium and saccharification powder are mixed in proportion, add 5 times to the water of above-listed nutrition weight, and add enzyme, regulate pH, between temperature setting 25 ~ 35 DEG C, after enzymolysis 28 ~ 36h, be placed in 4000r/30min centrifugal, get supernatant liquor, for subsequent use;
2) Chinese medicine composition is levigate, and cross 30 mesh sieves, obtain powder mix 15 parts, white sugar 25 parts, fructose 10 5g, water purification 350ml is mixed into saccharified liquid, adds the yeast 0.5ml cultivated on potato culture in advance, 15 ~ 25 DEG C of indirect fermentations 4 ~ 6 days, obtains fermented liquid;
3) selecting yeast culture medium prescription: potato 30%, glucose 1.5%, peptone 0.5%, magnesium sulfate 0.1%, calcium chloride 0.1%, KH 2pO 40.05%, inoculate under aseptic condition after cooling under a certain pressure;
4) fermented liquid adds the mixing supernatant 2.6L after enzymolysis, kill to live → filter after 15 ~ 25 DEG C of indirect fermentations → leave standstill → seasoning → finished product.
2. a kind of method preparing nutriment wine with bacterium, algae, stem of noble dendrobium composition as claimed in claim 1, it is characterized in that, the weight part of described each material is: the stem of noble dendrobium 20 ~ 35 parts, spirulina 20 ~ 40 parts, Chinese medicine composition 5 ~ 10 parts, yeast culture medium 10 ~ 25 parts, enzyme 5 ~ 15 parts, 8 ~ 15 parts, saccharification powder.
3. a kind of method preparing nutriment wine with bacterium, algae, stem of noble dendrobium composition as claimed in claim 1, it is characterized in that, described Chinese medicine composition is the mixture of Radix Glycyrrhizae, matrimony vine, red date, Poria cocos.
4. a kind of method preparing nutriment wine with bacterium, algae, stem of noble dendrobium composition as claimed in claim 1, it is characterized in that, described enzyme is hemicellulase, cellulase, saccharifying enzyme and proteolytic enzyme.
5. a kind of method preparing nutriment wine with bacterium, algae, stem of noble dendrobium composition as claimed in claim 1, is characterized in that, described step 1) in pH=5.0 ~ 7.8.
6. a kind of method preparing nutriment wine with bacterium, algae, stem of noble dendrobium composition as claimed in claim 1, is characterized in that, described step 1) in temperature be 30 DEG C.
7. a kind of method preparing nutriment wine with bacterium, algae, stem of noble dendrobium composition as claimed in claim 1, is characterized in that, described step 1) in enzymolysis time be 32h.
8. a kind of method preparing nutriment wine with bacterium, algae, stem of noble dendrobium composition as claimed in claim 1, is characterized in that, described step 3) in pressure be 1.176 × 10 5pa.
9. a kind of method preparing nutriment wine with bacterium, algae, stem of noble dendrobium composition as claimed in claim 1, is characterized in that, described step 4) in fermentation time be 2 ~ 5 days.
CN201510944440.7A 2015-12-16 2015-12-16 Method for preparing nutritional wine by using composition of fungi, algae and dendrobium Pending CN105349342A (en)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6119479A (en) * 1984-07-05 1986-01-28 Daiichi Seimo Kk Preparation of agar liquor
CN102634431A (en) * 2012-04-24 2012-08-15 汪永辉 Method for brewing dendrobium nobile wine
CN103103057A (en) * 2012-12-18 2013-05-15 徐州鸿宇农业科技有限公司 Preparation method of silkworm caterpillar fungus nutritive liqueur
JP2013183662A (en) * 2012-03-07 2013-09-19 Fisheries Research Agency Method for producing alcoholic beverage from aquatic plant resource, and product

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6119479A (en) * 1984-07-05 1986-01-28 Daiichi Seimo Kk Preparation of agar liquor
JP2013183662A (en) * 2012-03-07 2013-09-19 Fisheries Research Agency Method for producing alcoholic beverage from aquatic plant resource, and product
CN102634431A (en) * 2012-04-24 2012-08-15 汪永辉 Method for brewing dendrobium nobile wine
CN103103057A (en) * 2012-12-18 2013-05-15 徐州鸿宇农业科技有限公司 Preparation method of silkworm caterpillar fungus nutritive liqueur

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Application publication date: 20160224