CN105331539A - Cell cultivation model for artificial cornea reconstruction - Google Patents
Cell cultivation model for artificial cornea reconstruction Download PDFInfo
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- CN105331539A CN105331539A CN201510909305.9A CN201510909305A CN105331539A CN 105331539 A CN105331539 A CN 105331539A CN 201510909305 A CN201510909305 A CN 201510909305A CN 105331539 A CN105331539 A CN 105331539A
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- carrier bracket
- cell
- cell seeding
- model
- support housing
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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Abstract
The invention discloses a cell cultivation model for artificial cornea reconstruction. The cell cultivation model comprises a carrier bracket for cell cultivation and a supporting shell for supporting the carrier bracket. According to the designed model, firstly the carrier bracket is fixed to the supporting shell made of silica gel, then the carrier bracket is placed in a culture dish for cell cultivation, the supporting shell has a good supporting function on the carrier bracket, especially a thin-layer bracket, and the carrier bracket can be not prone to displacement and curl in the cultivation process.
Description
Technical field
The present invention relates to medical research technical field, be specifically related to a kind of when rebuilding for tissue engineering artificial corneal, any carrier bracket is planted the model of endothelial cell and/or corneal epithelial cell
Background technology
Cornea is positioned at eyes hyaline tissue foremost, is made up of epithelium layer, hypothallus and endothelial layer.Corneal epithelial cell (cornealepithelialcells), due to its specific position and structure, is easy to be subject to microorganism invasion, mechanical trauma, chemical burn and heating power scald etc.Limbal stem cell (corneallimbalepithelialcells) is the source that corneal epithelial cell upgrades and regenerates.Much serious eye surface diseases such as Stevens-Johnson syndrome and eye cicatricial pemphigoid, ocular infection and wound etc. all can cause limbal stem cell deficiency, and then cause corneal epithelium to lose regeneration and repair ability and blinding.Cornea innermost layer is made up of individual layer endothelial cell (cornealendothelialcells), its physical barriers and ionic pump function are most important for maintenance cornea normal thickness, half dewatering state and the transparency, and ECD must remain on the permeability that threshold value 400-500/more than mm2 could maintain cornea.
1,2because endothelial cell in body is bred limited in vivo, the major way of corneal endothelial layer injury repairing is the cell migration of the increase of endotheliocyte volume and periphery, loss too much can cause corneal edema, the transparency declines and then affects visual function, even causes corneal endothelium blind.
3according to the statistics of the World Health Organization, the annual whole world has 4,900,000 patients because keratopathy and blinding.For this part patient, corneal transplantation is unique healing means.In this populous nation of China, illness in eye is blind about 1,233 ten thousand people, accounts for 18% of whole world blind person's sum, is the country that blind person is maximum in the world.Wherein, keratopathy is the second largest diseases causing blindness of China, corneal blindness is China's the most scabrous bottleneck at present, patient is more than 3,000,000 people, and annual newly-increased 200,000 people, but be subject to the restriction of the factor such as shortage and donor's cornea cell inferior quality of donor's cornea material, be often only now about 10,000 people and recovered lost eyesight by corneal graft.
4
Nearly 5 years, China obtained comparatively great achievement in the research of the basis of keratopathy and clinicing aspect, and especially in tissue engineering comea research, clinical study has achieved good Preliminary Results.Tissue engineering comea is redeveloped into corneal blindness patient and brings new hope.The key of rebuilding utilizes seed cell and carrier bracket to build artificial cornea.The acquisition of current seed cell is quite ripe, carries out extensively for carrier bracket carries out corneal cell cultivation with various material with the experimental study transplanted.
5-8but, present stage the unripe and cell seeding model of specification.Major part research is directly positioned in culture dish by carrier bracket to carry out cell seeding.Use this implantation methods cell when rack surface repopulating cell easily to divide a word with a hyphen at the end of a line, grow to the another side of carrier bracket, the purity of cell cannot be ensured; And the corneal cell of easy damaged rack surface when gripping operates, causes cells in vivo density to decline.
9,10in addition, carrier bracket is in order to keep splendid transparency and suitable mechanical attributes, and it is inadequate that majority all has hardness, the shortcoming of repopulating cell and graft procedure difficulty.When particularly carrying out composition corneal transplantation, because only rebuild the cornea of segment thickness, so required carrier bracket thickness is very thin, during re-endothelialization, backing thickness is only 100 μm.So fragile carrier bracket carries out cell seeding and operates the addition difficult that seems.The gripping, upset etc. of support are processed to the cell even support of all very easily injured surface itself.And the effect of repopulating cell directly can affect the result for the treatment of that artificial cornea is transplanted in vivo.Even employ excellent seed cell, if cell purity and density do not reach requirement in planting process, the failure of transplanting all can be caused.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of novel cell seeding model, with the displacement avoiding carrier bracket to occur in cell seeding process, curls problem.
For solving the problems of the technologies described above, the invention discloses a kind of cell seeding model rebuild for artificial cornea, comprising one for the carrier bracket and of cell seeding for supporting the support housing of described carrier bracket.
Further improvement of the present invention is, described support housing comprises one first body and one second body, the outer rim of described carrier bracket forms a FX, described FX is fixedly clamped between described first body and the mouth of pipe of described second body, described carrier bracket just forms one first growing surface to the surface of described first body, and described carrier bracket just forms one second growing surface to the surface of described second body.
Further improvement of the present invention is, described support housing comprises one the 3rd body further, and described 3rd body is sheathed on outside described first body and described second body, and is coated and fixed on described FX.
Further improvement of the present invention is, described carrier bracket and described support housing are placed in a culture dish.
Further improvement of the present invention is, described FX is a closed ring region.
Further improvement of the present invention is, the width in described closed ring region is 1-2mm.
Model designed by the present invention is by being first fixed in the support housing of silica gel material by carrier bracket, and then be placed in culture dish and carry out cell seeding, support housing has good supporting role to carrier bracket especially thin layer support, carrier bracket in planting process can be made not easily to be shifted, to curl, and make the cell of planting also cannot travel to another growing surface of carrier bracket by the isolation of the first body and the second body, density and the purity of repopulating cell can be ensured.Meanwhile, the artificial cornea of this model construction has the annular FX of 1-2mm, and it can as operational zone in subsequent operations, and the operation such as gripping completes by annular FX, the corneal cell of not easily injured surface plantation.The more important thing is, by overturning simply this implant cast, the plantation of corneal endothelium and corneal epithelium can be completed simultaneously.Whole process need not operate carrier bracket separately, and little to the cell interference of plantation, success ratio is high.
Accompanying drawing explanation
Fig. 1 is the packaging assembly schematic diagram of cell seeding model of the present invention.
Fig. 2 is the STRUCTURE DECOMPOSITION schematic diagram of cell seeding model of the present invention.
Fig. 3 is the operating process schematic diagram of cell seeding model of the present invention.
Embodiment
For the benefit of to the understanding of principle of work of the present invention, be described below in conjunction with drawings and Examples.
With reference to shown in Fig. 1,2, the cell seeding model 1 for artificial cornea reconstruction of the present invention mainly comprises carrier bracket 10 and a support housing 20, wherein:
Carrier bracket 10 is for cell seeding, it comprises one first growing surface 11 and one second growing surface 12, the outer rim being positioned at the first growing surface 11 and the second growing surface 12 is provided with an annular FX 13 closed, as better embodiment of the present invention, this annular FX 13 width design closed is 1-2mm.
Support housing 20 is for prop carrier support 10, it comprises the first body 21, one second body 22 and one the 3rd body 23, above-mentioned FX 13 sandwiched be fixed on state the first body 21 and described second body 22 the mouth of pipe between thus supporting role is formed to carrier bracket 10, make it can not be shifted, curl, and keep the first growing surface 11 and one second growing surface 12 shaping, the first growing surface 11 just to the first body 21, second growing surface 12 just to the second body 22; Further, the 3rd body 23 is sheathed on outside described first body 21 and described second body 22, and is coated and fixed on described FX 13; As better embodiment of the present invention, this first body 21, second body 22 and the 3rd body 23 adopt silica gel material.
Coordinate described in Fig. 3, cell seeding model 1 of the present invention is when operating, first prop carrier support 10 is fixedly clamped in the first body 21, on second body 22 and the 3rd body 23, then prop carrier support 10 is placed in a culture dish 30 together with support housing 20, modes of emplacement is first by the second body 22 down, makes its mouth of pipe be supported in the bottom of culture dish 30; Then, in the first body 21, instill culture medium 40, the first growing surface 11 carries out the plantation of endothelial cell 50; 5-7 days after planting, overturns this cell seeding model 1, makes the first body 21 down and is supported in the bottom of culture dish 30, now in the second body 22, instilling culture medium 40, the second growing surface 12 carries out the plantation of corneal epithelial cell 60.
The present invention carries out cell seeding after using silicone tube model to be fixed carrier bracket again, and can play good supporting role to various support especially thin layer support, in planting process, carrier bracket is not easily shifted, curls; And because the iris action of silicone tube, the cell of plantation cannot travel to the another side of carrier bracket, ensure that the purity of cell.Simultaneously, because the carrier bracket of silicone tube FX does not have the plantation of cell, the artificial cornea of this model construction will have the annular operational zone of 1-2mm, go to carry out when gripping and graft procedure by annular, and the corneal cell of not easily injured surface plantation.The more important thing is, by overturning simply this implant cast, successively can complete corneal endothelium and the common plantation of corneal epithelium on support.Whole process operates carrier bracket without gripping, and little to the interference of repopulating cell, success ratio is high.Really have planting effect good, make simple and convenient, economical and practical, with low cost, the simple advantage of structure.
Below embodiment is to invention has been detailed description by reference to the accompanying drawings, and those skilled in the art can make many variations example to the present invention according to the above description.Thus, some details in embodiment should not form limitation of the invention, the present invention by the scope that defines using appended claims as protection domain.
Claims (7)
1., for the cell seeding model that artificial cornea is rebuild, it is characterized in that comprising one for the carrier bracket and of cell seeding for supporting the support housing of described carrier bracket.
2. cell seeding model as claimed in claim 1, it is characterized in that: described support housing comprises one first body and one second body, the outer rim of described carrier bracket forms a FX, described FX is fixedly clamped between described first body and the mouth of pipe of described second body, described carrier bracket just forms one first growing surface to the surface of described first body, and described carrier bracket just forms one second growing surface to the surface of described second body.
3. cell seeding model as claimed in claim 2, it is characterized in that: described support housing comprises one the 3rd body further, described 3rd body is sheathed on outside described first body and described second body, and is coated and fixed on described FX.
4. cell seeding model as claimed in claim 1, is characterized in that: described carrier bracket and described support housing are placed in a culture dish.
5. cell seeding model as claimed in claim 2, is characterized in that: described FX is a closed ring region.
6. cell seeding model as claimed in claim 2, is characterized in that: the width in described closed ring region is 1-2mm.
7. cell seeding model as claimed in claim 1, is characterized in that: support housing is silica gel material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510909305.9A CN105331539A (en) | 2015-12-10 | 2015-12-10 | Cell cultivation model for artificial cornea reconstruction |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510909305.9A CN105331539A (en) | 2015-12-10 | 2015-12-10 | Cell cultivation model for artificial cornea reconstruction |
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| CN105331539A true CN105331539A (en) | 2016-02-17 |
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| CN201510909305.9A Pending CN105331539A (en) | 2015-12-10 | 2015-12-10 | Cell cultivation model for artificial cornea reconstruction |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020247420A1 (en) * | 2019-06-03 | 2020-12-10 | Advanced Solutions Life Sciences, Llc | System and method for fabricating a cornea |
| CN114369532A (en) * | 2021-12-31 | 2022-04-19 | 中国科学院苏州纳米技术与纳米仿生研究所 | Micro-fluidic cell culture device for simulating filtering bubbles and application thereof |
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| KR20050117690A (en) * | 2004-06-11 | 2005-12-15 | 주식회사 바이오랜드 | Device for holding a membrane used in culturing cells |
| CN102807965A (en) * | 2012-08-28 | 2012-12-05 | 陕西瑞盛生物科技有限公司 | Method for preparing tissue engineered cornea and device of method |
| CN105238691A (en) * | 2015-11-13 | 2016-01-13 | 张志华 | Cell plantation model for artificial cornea reconstruction |
-
2015
- 2015-12-10 CN CN201510909305.9A patent/CN105331539A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20050117690A (en) * | 2004-06-11 | 2005-12-15 | 주식회사 바이오랜드 | Device for holding a membrane used in culturing cells |
| CN102807965A (en) * | 2012-08-28 | 2012-12-05 | 陕西瑞盛生物科技有限公司 | Method for preparing tissue engineered cornea and device of method |
| CN105238691A (en) * | 2015-11-13 | 2016-01-13 | 张志华 | Cell plantation model for artificial cornea reconstruction |
| CN106047699A (en) * | 2015-11-13 | 2016-10-26 | 上海市第人民医院 | Cell implantation model for artificial corneal reconstruction |
Non-Patent Citations (1)
| Title |
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| 张超等: "组织工程角膜生物材料载体制备的比较性研究", 《中国修复重建外科杂志》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020247420A1 (en) * | 2019-06-03 | 2020-12-10 | Advanced Solutions Life Sciences, Llc | System and method for fabricating a cornea |
| US11571496B2 (en) | 2019-06-03 | 2023-02-07 | Advanced Solutions Life Sciences, Llc | System and method for fabricating a cornea |
| CN114369532A (en) * | 2021-12-31 | 2022-04-19 | 中国科学院苏州纳米技术与纳米仿生研究所 | Micro-fluidic cell culture device for simulating filtering bubbles and application thereof |
| CN114369532B (en) * | 2021-12-31 | 2023-10-03 | 中国科学院苏州纳米技术与纳米仿生研究所 | Microfluidic cell culture device simulating filtration bleb and application thereof |
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Application publication date: 20160217 |