CN105325333A

CN105325333A - Method for artificially hatching river crab eggs

Info

Publication number: CN105325333A
Application number: CN201510722386.1A
Authority: CN
Inventors: 蒋敏
Original assignee: Yingshang Runhai Livestock And Poultry Feeding Co Ltd
Current assignee: Yingshang Runhai Livestock And Poultry Feeding Co Ltd
Priority date: 2015-10-31
Filing date: 2015-10-31
Publication date: 2016-02-17

Abstract

The invention relates to the technical field of aquaculture, in particular to a method for artificially hatching river crab eggs. The method comprises the steps of collecting fertilized eggs laid from genital pores of mother river crabs after spawning by mating of the mother river crabs; enabling a hatching solution to fill a hatching pond, and moving the fertilized eggs into the hatching pond; irradiating the fertilized eggs by He-Ne laser during a hatching process; inoculating diatom in a cultivating pond; moving zoaea larvas into the cultivating pond after the fertilized eggs grow into the zoaea larvas, and irradiating the zoaea larvas for 10 to 12 hours every day by using a fluorescent lamp of which the illumination intensity is 1500 to 1970 lx during a cultivating process; evacuating megalopas into other cultivating ponds after the zoaea larvas grow into the megalopas; during a cultivating period, mixing and twisting shrimp meal, fish meal, bone meal and glutinous rice flour into particles of which the particle diameter is smaller than 1mm by adding water according to a weight ratio of 1 to 2 to 0.2 to 0.5, and applying and spreading the particles into the cultivating ponds for twice a day and 500 to 800 g per square meter for once; moving young crabs into a fresh water zone after the megalopas grow into the young crabs.

Description

A kind of river crab ovum artificial incubation method

Technical field

The present invention relates to technical field of aquaculture, be specifically related to a kind of river crab ovum artificial incubation method.

Background technology

River crab also cries " crab " and " eriocheir sinensis ", and be a kind of large-scale crustacean, the carapace that head and chest are combined into is square circular, and quality is hard.Front end body has a pair, side has two to very hard sharp crab tooth, crab a pair appendage is foremost the foot of a chela, surface covers with fine hair, 4 pairs of steps are had after the foot of a chela, flat-sided and longer, abdominal appendage is degenerated, the male and female of river crab can distinguish from its belly, female belly is rounded, male belly is triangle, river crab often lives in caves in river, river, the mud bank of lakes and marhshes, nocturnalism, with fish, shrimp, spoil and cereal are food, annual autumn, normal migration was laid eggs to the river mouth gone to sea, Second Year hatching in 3 ~ May, after developing into young crab, trace back again rivers and on, educate at fresh water relaying supervention and grow up.River crab Fresh & Tender in Texture is the food be simply loved by the people.River crab grows in fresh water, breed in the seawater, after juvenile crab enters fresh water, generally in the river bend that bait is abundant, live about 18 months in lake, sexual gland is ripe gradually, ripe river crab started to lay eggs to the neritic zone of sea water and fresh water mixing in the time of the year when autumn changes into winter, breeding migration that Here it is, the female crab of post-coitum just can lay eggs soon, ovum is a string to be attached on the abdominal appendage hair of female solution, be deposited in belly, until hatchling, although river crab once can produce tens thousand of to 1,000,000 ovum, but female crab abdominal space is limited, incubating oosperm quantity is also limited, and just to can grow up to juvenile crab through 4 months in natural world fertilized egg.

Summary of the invention

In order to solve river crab oogenesis nursery in prior art, to there is the cycle long, and hatching survival rate is affected by environment comparatively greatly, and the unmanageable shortcoming of the young after hatching, the invention provides a kind of river crab ovum artificial incubation method.

The technical solution used in the present invention is, a kind of river crab ovum artificial incubation method, and its incubation step is as follows:

After female river crab mate and oviposit, collect the fertilized egg discharged from female crab gonopore;

Artemia hatching solution is passed in hatchery, fertilized egg is moved to hatchery, hatching density is 3-5 grain/ml, in hatchery, dissolved oxygen remains on 10-12mg/L, incubation temperature remains on 23-26 DEG C, and artemia hatching solution is mixed by the weight ratio of 100:5:0.2:0.5:5:8:2:0.1:7 by water, protein hydrolysate, alpha-linolenic acid, B B-complex, glucose, sodium chloride, calcium gluconae, zinc sulphate, sodium alginate;

In hatching process, use he-ne laser irradiation fertilized egg, operation wavelength is 632.8nm, and laser output power is 5mW;

Diatom is inoculated in raising pond, inoculum density is 8000-1 ten thousand/mL, after development of fertilized ova becomes Magna zoea larva, Magna zoea larva is moved to and brings up pond, bringing up lake salt degree is 15%-18%, and raising temperature is 22-25 DEG C, and bringing up density is 2000-3000/square metre, bring up pond dissolved oxygen and remain on 10-12mg/L, in raising process, intensity of illumination is that the fluorescent lamp of 1500-1970lx irradiates Magna zoea larva 10-12 hour every day;

After Magna zoea larva develops into megalops larva, megalops larva is evacuated in all the other raising ponds, bringing up density is 300-500/square metre, raising salinity is 10%-12%, raising temperature is 14-16 DEG C, bring up pond dissolved oxygen and remain on 18-20mg/L, in raising process, intensity of illumination is that the fluorescent lamp of 2000-2570lx irradiates megalops larva 10-12 hour every day, during raising, by shrimp med, fish meal, bone meal, glutinous rice flour kneads into by the weight ratio mixing and water adding of 1:2:0.2:0.5 the graininess that particle diameter is below 1mm, execute and spill in culturing pool, execute every day and spill 2 times, execute at every turn and spill 500-800g/ square metre,

Change in quality into after young crab until megalops larva, young crab is moved to fresh water area.

The present invention adopts artificial incubation crab ovum, for fertilized egg provides unified environment of hatching, improve crab egg hatching rate and hatching survival rate, hatching survival rate can reach 98.6%, and artificial incubation simultaneously also shortens the crab egg hatching cycle, is easy to management between the incubation period, Magna zoea larva can be developed into through 8-10 days after fertilized egg moves to hatchery, Magna zoea larva can develop into megalops larva through cultivation in about 15 days, and megalops larva can develop into young crab after cultivating through 3-5 days.

Embodiment

Artemia hatching solution is passed in hatchery, fertilized egg is moved to hatchery, hatching density is 4/ml, in hatchery, dissolved oxygen remains on 11mg/L, incubation temperature remains on 25 DEG C, and artemia hatching solution is mixed by the weight ratio of 100:5:0.2:0.5:5:8:2:0.1:7 by water, protein hydrolysate, alpha-linolenic acid, B B-complex, glucose, sodium chloride, calcium gluconae, zinc sulphate, sodium alginate;

Diatom is inoculated in raising pond, inoculum density 9000/mL, after development of fertilized ova becomes Magna zoea larva, Magna zoea larva is moved to and brings up pond, bringing up lake salt degree is 16%, and bringing up temperature is 24 DEG C, and bringing up density is 2500/square metre, bring up pond dissolved oxygen and remain on 11mg/L, in raising process, intensity of illumination is that the fluorescent lamp of 1800lx irradiates Magna zoea larva 11 hours every day;

After Magna zoea larva develops into megalops larva, megalops larva is evacuated in all the other raising ponds, it is 400/square metre that megalops larva brings up density, it is 11% that megalops larva brings up salinity, it is 15 DEG C that megalops larva brings up temperature, bring up pond dissolved oxygen and remain on 19mg/L, in megalops larva raising process, intensity of illumination is that the fluorescent lamp of 2400lx irradiates megalops larva 11 hours every day, during megalops larva is brought up, by shrimp med, fish meal, bone meal, glutinous rice flour kneads into by the weight ratio mixing and water adding of 1:2:0.2:0.5 the graininess that particle diameter is below 1mm, execute and spill in culturing pool, execute every day and spill 2 times, execute at every turn and spill 600g/ square metre, change in quality into after young crab until megalops larva, young crab is moved to fresh water area.

Claims

1. a river crab ovum artificial incubation method, is characterized in that, its incubation step is as follows:

After a, female river crab mate and oviposit, collect the fertilized egg discharged from female crab gonopore;

B, artemia hatching solution is passed in hatchery, fertilized egg is moved to hatchery, hatching density is 3-5 grain/ml, in hatchery, dissolved oxygen remains on 10-12mg/L, incubation temperature remains on 23-26 DEG C, and artemia hatching solution is mixed by the weight ratio of 100:5:0.2:0.5:5:8:2:0.1:7 by water, protein hydrolysate, alpha-linolenic acid, B B-complex, glucose, sodium chloride, calcium gluconae, zinc sulphate, sodium alginate;

C, in hatching process, use he-ne laser irradiation fertilized egg, operation wavelength is 632.8nm, and laser output power is 5mW;

D, raising pond in inoculate diatom, inoculum density is 8000-1 ten thousand/mL, after development of fertilized ova becomes Magna zoea larva, Magna zoea larva is moved to and brings up pond, bringing up lake salt degree is 15%-18%, and raising temperature is 22-25 DEG C, and bringing up density is 2000-3000/square metre, bring up pond dissolved oxygen and remain on 10-12mg/L, in raising process, intensity of illumination is that the fluorescent lamp of 1500-1970lx irradiates Magna zoea larva 10-12 hour every day;

E, after Magna zoea larva develops into megalops larva, megalops larva is evacuated in all the other raising ponds, bringing up density is 300-500/square metre, raising salinity is 10%-12%, raising temperature is 14-16 DEG C, bring up pond dissolved oxygen and remain on 18-20mg/L, in raising process, intensity of illumination is that the fluorescent lamp of 2000-2570lx irradiates megalops larva 10-12 hour every day, during raising, by shrimp med, fish meal, bone meal, glutinous rice flour kneads into by the weight ratio mixing and water adding of 1:2:0.2:0.5 the graininess that particle diameter is below 1mm, execute and spill in culturing pool, execute every day and spill 2 times, execute at every turn and spill 500-800g/ square metre,

F, change in quality into after young crab until megalops larva, young crab is moved to fresh water area.