CN105288720B - Bonding proximate matter for vascular anastomosis - Google Patents
Bonding proximate matter for vascular anastomosis Download PDFInfo
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- CN105288720B CN105288720B CN201510675300.4A CN201510675300A CN105288720B CN 105288720 B CN105288720 B CN 105288720B CN 201510675300 A CN201510675300 A CN 201510675300A CN 105288720 B CN105288720 B CN 105288720B
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Abstract
This case is related to a kind of bonding proximate matter for vascular anastomosis, including absorbed layer and adhesive layer；Wherein, the absorbed layer includes polylactide glycolic acid copolymer, albumin and sterile water；The adhesive layer includes albumin, chitosan, hydroxypropyl methyl cellulose, gelatin, sterile water and optical absorption dyestuff, arbitrary two kind combination of the optical absorption dyestuff in indoles cyanogen chlorine, methylene blue, carbon black, flower cyanines uniformly dyeing material CY7.5 and Alexa Fluor 790.The optical absorption dyestuff of two kinds of different absorbing wavelengths is carried out organic assembling by this case, it makes it possible to cooperation dual-wavelength laser and carries out vascular anastomosis, two kinds of different principles of different laser penetration depth are used, in the energy progress vascular anastomosis that most more can be preferably provided close to previous anastomotic position, two kinds of dyestuff synergistic effects using laser.
Description
Technical field
The present invention relates to a kind of jointing material for vascular anastomosis, more particularly to what a kind of cooperation dual-wavelength laser used
Bonding proximate matter for laser vascular anastomosis operation.
Background technology
Vascular anastomosis refers to that will be broken ends of vessels connection, is allowed to restore again unobstructed operation method.In recent years, with
The incidence such as vasodilation disorders and vascular occlusive disease and injury of blood vessel gradually increase, to vasotransplantation, blood vessel
More stringent requirements are proposed for the technology of suture and repairing.The anastomosis of thin vessels and capilary is directly related to blood vessel distal end
Histoorgan can survive, occupied an important position in the fields such as vascular surgery and microsurgery.It is continuous with microsurgery
Development, the requirement to vascular anastomosis technology are being continuously improved, not only to have good instant patency rate, also to there is better long term
Patency rate and anti-drawing tension etc..
The identical common method of clinical vascular is sewing, and this method is suitble to large and mediumsize vessel, but in suture diameter
When tiny blood vessels less than lmm, not only difficulty is big, but also measures more, time-consuming, leverages the success rate of operation and wider
Using.People are with keen interest to that can simplify surgical procedure, the mis instruments equipment for shortening operating time and technical method, and then go out
A series of new vascular anastomosis method is showed.For example, blood vessel anastomosis clamp/skill of handling needles, tissue glue's method, tiretube process and laser vascular anastomosis
Method etc..
There is laser vascular anastomosis art the end of the seventies, the technology one appearance is attract attention immediately.Using sharp
When light carries out vascular anastomosis, the heat effect of laser can be such that albuminous degeneration in broken ends of fractured bone vascular cell cures, and then stick together.
The penetration depth of laser is shallower, can reduce the damage to endangium, is conducive to the reparation and reconstruction of blood vessel.Although people carry out real
It is different to test the technical equipment of research, experimental design, research object, observation detection means and result, but obtains consistent recognizing
Together.I.e. laser vascular anastomosis art is a kind of identical method of minimally-invasive vascular, and potential clinical value is huge.
Vascular anastomosis is carried out using laser to has the following advantages compared with traditional suture：First, operation is simple, and tissue lacks
The blood time is short.The 1/3-1/4 that the time needed for every blood vessel is about hand stitch is welded, it is easily operated；Secondly, to injury of blood vessel
It is small, it is conducive to the post-operative recovery of blood vessel.Reduce suture use so that reduce to the damage of blood vessel, while blood vessel foreign body reaction journey
Degree mitigates significantly, is conducive to the recovery of post-surgical vascular；Finally, vascular patency is high, can effectively reduce granulation tissue hyperplasia, significantly
The possibility that previous anastomotic local anomaly blood flow is formed is reduced, is conducive to keep blood vessel unobstructed for a long time.
When application laser carries out vascular anastomosis, in addition to the laser used, it is also necessary to which a certain amount of identical agent cooperation is completed.
These agent that coincide are largely bioadhesive, for example fibrin, fibrinogen etc., but the effect of these adhesives is inadequate
Ideal, it is best in order to make laser vascular anastomosis achieve the effect that, also require accurate alignment previous anastomotic edge.And blood vessel is flexible
Structure cannot achieve accurate docking, this will have a direct impact on identical quality.Therefore, it using necessary blood vessel inner supporting structure, carries
The quality for rising vascular anastomosis is necessary.But when carrying out vascular anastomosis just with the laser of single wavelength, work as optical maser wavelength
Long (1 μm or more), the heat effect of laser is fairly obvious, and penetration depth is too shallow, is unfavorable for coincideing for blood vessel deep tissues；And if
Using short wavelength laser (such as 532nm), its penetration depth is although deep, but for superficial vein function and effect unobvious,
Therefore, the two is organically combined and is just provided with meaning.
Invention content
For the shortcomings of the prior art, it is an object of the invention to be directed to dual-wavelength laser anastomosis of blood vessel
Develop a matched bonding proximate matter.
To achieve the above object, the invention is realized by the following technical scheme：
A kind of bonding proximate matter for vascular anastomosis, including absorbed layer and adhesive layer；
Wherein, the absorbed layer includes Poly(D,L-lactide-co-glycolide, albumin and sterile water；
The adhesive layer includes albumin, chitosan, hydroxypropyl methyl cellulose, gelatin, sterile water and optical absorption
Dyestuff, the optical absorption dyestuff are selected from indoles cyanogen chlorine, methylene blue, carbon black, flower cyanines uniformly dyeing material CY7.5 and Alexa Fluor
Arbitrary two kinds of combinations in 790.
Preferably, the bonding proximate matter for vascular anastomosis, wherein in the absorbed layer, polylactic acid-glycolic base second
The quality volume fraction of acid copolymer is 10~15%.
Preferably, the bonding proximate matter for vascular anastomosis, wherein in the absorbed layer, the quality of albumin
Volume fraction is 30~45%.
Preferably, the bonding proximate matter for vascular anastomosis, wherein in the adhesive layer, the quality of albumin
Volume fraction is 48~54%.
Preferably, the bonding proximate matter for vascular anastomosis, wherein in the adhesive layer, the quality of chitosan
Volume fraction is 3~8%.
Preferably, the bonding proximate matter for vascular anastomosis, wherein in the adhesive layer, hydroxypropyl methyl is fine
The quality volume fraction of dimension element is 1~2.5%.
Preferably, the bonding proximate matter for vascular anastomosis, wherein in the adhesive layer, the mass body of gelatin
Fraction is 1~1.5%.
Preferably, the bonding proximate matter for vascular anastomosis, wherein in the adhesive layer, optical absorption dyestuff
Quality volume fraction be 0.25~3%.
The beneficial effects of the invention are as follows：The optical absorption dyestuff of two kinds of different absorbing wavelengths is carried out organic assembling by this case,
It makes it possible to cooperation dual-wavelength laser and carries out vascular anastomosis, used two kinds of different principles of different laser penetration depth,
In the energy progress blood vessel kiss that most more can be preferably provided close to previous anastomotic position, two kinds of dyestuff synergistic effects using laser
It closes.
Description of the drawings
Fig. 1 is the structural schematic diagram of the bonding proximate matter for vascular anastomosis.
Specific implementation mode
Present invention will be described in further detail below with reference to the accompanying drawings, to enable those skilled in the art with reference to specification text
Word can be implemented according to this.
Fig. 1 is referred to, this case provides the bonding proximate matter for vascular anastomosis of an embodiment, including absorbed layer 1 and bonding
Layer 2；This case to the specific wavelength selection of dual-wavelength laser there is no limit, can be selected according to actual needs, the present embodiment with
Wavelength be 808nm and 1064nm laser as a reference example.
Wherein, absorbed layer 1 includes Poly(D,L-lactide-co-glycolide, albumin and sterile water；
Adhesive layer 2 includes albumin, chitosan, hydroxypropyl methyl cellulose, gelatin, sterile water and optical absorption dye
Material, the optical absorption dyestuff are selected from indoles cyanogen chlorine, methylene blue, carbon black, flower cyanines uniformly dyeing material CY7.5 and Alexa Fluor 790
In it is arbitrary two kinds combination.For example, optical absorption dyestuff can be the combination of indoles cyanogen chlorine and carbon black, can also be carbon black and flower
The combination of cyanines uniformly dyeing material CY7.5, but the accounting of two kinds of dye combinations can not be limited, because the ratio of the two is can basis
The actual needs of anastomosis of blood vessel carry out it is freely regulated, made by adjust also be a kind of adjustment of adaptability.
Although all containing water in absorbed layer 1 and adhesive layer 2, it is a kind of solution to seem, actually depositing due to polymer
So that its entirety is rendered as a kind of semisolid paste state, is based on this, it can just be made as a kind of bonding proximate matter.It is viscous
Optical absorption dyestuff in knot layer 2 has been primarily involved in absorption laser energy, and is translated into thermal energy, is the anastomosis procedures of blood vessel
Most direct thermal energy is provided.The thermal energy of generation can be such that albumin, chitosan, hydroxypropyl methyl cellulose, gelatin is formed in turn
A kind of reticular structure of complexity simultaneously generates certain stickiness, provides adhesion strength for the identical of blood vessel, prevents the blood vessel after coincideing
There is a phenomenon where leak or rupture.Therefore, albumin, chitosan, hydroxypropyl methyl cellulose and gelatin are that collaboration is deposited each other
Indivisible organic whole plays an important role to the identical intensity of anastomosis of blood vessel.1 main component of absorbed layer is poly- breast
The mixture of acid-co-glycolic acid and albumin, both compounds more have rigidity, its function is as one
Protective layer is supported, the fracture and distortion of adhesive layer can be prevented.
In order to further increase effect of the above-mentioned formula to vascular anastomosis, need to limit each component content therein
It is fixed.
In the above-described embodiments, in absorbed layer 1, the quality volume fraction of Poly(D,L-lactide-co-glycolide (m/v ×
100%) it is preferably 10~15%.If exceeding this range, it will the semi-solid state for influencing absorbed layer influences its clothes to blood vessel
Paste effect.
In the above-described embodiments, in absorbed layer 1, the quality volume fraction of albumin is preferably 30~45%.If exceeding this
Range, it will the rigidity for influencing absorbed layer 1 makes it lose protective effect.
In the above-described embodiments, in adhesive layer 2, the quality volume fraction of albumin is preferably 48~54%.The albumin
Preferably it is bovine serum albumin(BSA).If the concentration of albumin exceeds preferred range, will cause fine with chitosan, hydroxypropyl methyl
The synergistic effect of dimension element and gelatin reduces, and so that stickiness is reduced, can not also form effectively firm network structure.
In the above-described embodiments, in adhesive layer 2, the quality volume fraction of chitosan is preferably 3~8%.If chitosan
Concentration exceeds preferred range, will cause to reduce with the synergistic effect of albumin, hydroxypropyl methyl cellulose and gelatin, makes stickiness
It reduces, can not also form effectively firm network structure.
In the above-described embodiments, in adhesive layer 2, the quality volume fraction of hydroxypropyl methyl cellulose is preferably 1~
2.5%.If the concentration of hydroxypropyl methyl cellulose exceeds preferred range, the association with albumin, chitosan and gelatin will be caused
Effect effect reduces, and stickiness is made to reduce, and can not also form effectively firm network structure.
In the above-described embodiments, in adhesive layer 2, the quality volume fraction of gelatin is preferably 1~1.5%.If gelatin is dense
Degree exceeds preferred range, will cause to reduce with the synergistic effect of albumin, chitosan and hydroxypropyl methyl cellulose, makes stickiness
It reduces, can not also form effectively firm network structure.
In the above-described embodiments, in adhesive layer 2, the quality volume fraction of optical absorption dyestuff is preferably 0.25~3%.If
Its content is relatively low, then enough heats are generated in formula system that can not be where it；If content is higher, heat mistake will be caused
Greatly, it has a negative impact to blood vessel surface tissue.
Although the embodiments of the present invention have been disclosed as above, but its is not only in the description and the implementation listed
With it can be fully applied to various fields suitable for the present invention, for those skilled in the art, can be easily
Realize other modification, therefore without departing from the general concept defined in the claims and the equivalent scope, the present invention is simultaneously unlimited
In specific details and legend shown and described herein.
Claims (6)
1. a kind of bonding proximate matter for vascular anastomosis, including absorbed layer and adhesive layer；
Wherein, the absorbed layer includes Poly(D,L-lactide-co-glycolide, albumin and sterile water；
The adhesive layer includes albumin, chitosan, hydroxypropyl methyl cellulose, gelatin, sterile water and optical absorption dyestuff,
The optical absorption dyestuff is in indoles cyanogen chlorine, methylene blue, carbon black, flower cyanines uniformly dyeing material CY7.5 and Alexa Fluor 790
Arbitrary two kinds of combinations；
In the absorbed layer, the quality volume fraction of Poly(D,L-lactide-co-glycolide is 10~15%；
In the adhesive layer, the quality volume fraction of albumin is 48~54%.
2. the bonding proximate matter according to claim 1 for vascular anastomosis, which is characterized in that in the absorbed layer, white egg
White quality volume fraction is 30~45%.
3. the bonding proximate matter according to claim 1 for vascular anastomosis, which is characterized in that in the adhesive layer, shell is poly-
The quality volume fraction of sugar is 3~8%.
4. the bonding proximate matter according to claim 3 for vascular anastomosis, which is characterized in that in the adhesive layer, hydroxypropyl
The quality volume fraction of ylmethyl cellulose is 1~2.5%.
5. the bonding proximate matter according to claim 4 for vascular anastomosis, which is characterized in that in the adhesive layer, gelatin
Quality volume fraction be 1~1.5%.
6. the bonding proximate matter according to claim 5 for vascular anastomosis, which is characterized in that in the adhesive layer, optics
The quality volume fraction for absorbing dyestuff is 0.25~3%.
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| CN201510675300.4A CN105288720B (en) | 2015-10-19 | 2015-10-19 | Bonding proximate matter for vascular anastomosis |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510675300.4A CN105288720B (en) | 2015-10-19 | 2015-10-19 | Bonding proximate matter for vascular anastomosis |
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| CN105288720A CN105288720A (en) | 2016-02-03 |
| CN105288720B true CN105288720B (en) | 2018-07-27 |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1935269A (en) * | 1998-06-18 | 2007-03-28 | 澳大利亚微研究基金会 | Compound for tissue repair and application thereof |
| CN102238971A (en) * | 2008-09-19 | 2011-11-09 | 宾夕法尼亚大学董事会 | Solder formulation and use in tissue welding |
| CN102989035A (en) * | 2011-09-10 | 2013-03-27 | 浙江海正药业股份有限公司 | Hydrogel applicable to angiostomy and preparation method thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8062330B2 (en) * | 2007-06-27 | 2011-11-22 | Tyco Healthcare Group Lp | Buttress and surgical stapling apparatus |
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2015
- 2015-10-19 CN CN201510675300.4A patent/CN105288720B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1935269A (en) * | 1998-06-18 | 2007-03-28 | 澳大利亚微研究基金会 | Compound for tissue repair and application thereof |
| CN102238971A (en) * | 2008-09-19 | 2011-11-09 | 宾夕法尼亚大学董事会 | Solder formulation and use in tissue welding |
| CN102989035A (en) * | 2011-09-10 | 2013-03-27 | 浙江海正药业股份有限公司 | Hydrogel applicable to angiostomy and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 激光血管吻合技术研究进展;尹焕才等;《现代生物医学进展》;20131230;第13卷(第36期);7179-7181、7149 * |
| 血管直接粘接吻合方法的实验研究;范恒华等;《中华显微外科杂志》;20070825;第30卷(第4期);291-293 * |
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