CN105277541B - Zinc ion field fast detection method in a kind of water for eliminating reagent blank influence - Google Patents
Zinc ion field fast detection method in a kind of water for eliminating reagent blank influence Download PDFInfo
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- CN105277541B CN105277541B CN201510061826.3A CN201510061826A CN105277541B CN 105277541 B CN105277541 B CN 105277541B CN 201510061826 A CN201510061826 A CN 201510061826A CN 105277541 B CN105277541 B CN 105277541B
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Abstract
The invention discloses zinc ion field fast detection method in a kind of water for eliminating reagent blank influence, this method by the preparation of liquid-type zinc ion detection reagent, deduct reagent blank color and to be compareed blank tube color addition with sample cell again on standard color card when making, detection of the transparent plastic sheet for the standard color card of support material, with the advantages of method is simple, accuracy is high, easily operated；Detected using liquid-type zinc ion detection reagent, the time required to can effectively controlling whole detection process；Method by the way that blank tube color addition to be carried out to colorimetric control with sample cell again on standard color card, the fluctuation that can effectively eliminate reagent blank adversely affect to caused by testing result accuracy, are particularly suitable for zinc ion field quick detection in water.
Description
Technical field
The invention belongs to zinc ion scene in field of water quality detection, more particularly to a kind of water for eliminating reagent blank influence
Quick determination method.
Background technology
Zinc is one of the essential trace elements of the human body, is the trace element that content is most in brain.Water source can be made by zinc pollution
Enter human body into zinc overdose.Zinacalism clinical manifestation is refractory anemia, loss of appetite, is associated with serum fatty acid and shallow lake
Powder enzyme increases.Long-term excess ingestion zinc can influence copper metabolism, cause low copper, and zinc, copper ratio are too high, can influence cholesterol metabolic,
Hypercholesterolemia is formed, and HDL is reduced 20%-25%, ultimately results in atherosclerosis, hypertension, hat
Worry.
The detection of zinc ion is more using atomic absorption spectrophotometry, visible spectrophotometry, inductive etc. in water
Plasma-atomic emission spectrum/mass spectrography, electrochemical methods etc., these methods are both needed to taking back water sample into special laboratory,
Chemically examined and completed by the operating technology personnel of specialty, most of method also needs to the high large-scale instrumentation of price, it is impossible to meets
Quickly, easy Site Detection requirement, some special when and wheres also in the presence of sampling not in time, sampling can not be objectively responded
The situation of live Water quality.
Existing zinc ion Site Detection reagent is mostly the dry-type detection reagent based on colorimetric method in the market,
The detection reagent takes a certain amount of water sample mix, dissolve with dry-type detection reagent when measuring, react a period of time after
Specific color is produced, the concentration for drawing water sample test substance is directly compareed with standard color card.Such method is quick, conveniently, it is public
Many participations are high, but influence of the change to measurement result of reagent blank can not be eliminated during measurement, only constant in reagent blank
Just there is higher accuracy when constant.But the detection reagent of actually detected discovery, either liquid-type or dry-type,
That reagent blank becomes larger or diminished in storing process all inevitably be present.In addition, the inspection using dry-type
, it is necessary to first detection reagent is fully dissolved with water sample to be measured when test agent carries out water sample detection, then with the specific pollution in water sample
Thing carries out reaction and produces particular color.Because the TWS that Various Seasonal, different zones gather is not quite similar, dry-type is dissolved
Also it is not quite similar the time required to during detection reagent, is difficult to effectively control the time required to whole detection process, also to testing result
Accuracy adversely affects.
Therefore, demand it is a kind of using liquid-type detection reagent, reagent blank can be eliminated testing result is influenceed, using standard
The detection method of zinc ion in the quantitative water of the direct visual colorimetric determination of colour atla, for realizing, zinc ion field quick detection seems in water
It is particularly important.
The content of the invention
The purpose of the embodiment of the present invention in a kind of water for eliminating reagent blank influence is provided zinc ion scene it is quick
Detection method, to realize the live quick, Accurate Determining of zinc ion in water, prior art is overcome when using dry-type detection reagent
When being difficult to effective control detection time because of the difference of dissolution time, not deducting standard color card, the colorimetric of reagent blank because using
This standard color card is directly compareed with sample cell obtain a result, the fluctuation that can not eliminate reagent blank is made to testing result accuracy
The shortcomings that into adverse effect..
The present invention is achieved in that zinc ion field fast detection method in a kind of water for eliminating reagent blank influence
Including：
Step 1: prepare liquid-type zinc ion detection reagent, the detection reagent be containing 2- (5- Bromo-2-pyridylazos)-
The trihydroxy methyl amino first that 5- diethylamino phenols, TritonX-100, calgon, sodium citrate, the pH of ethylene glycol are 8.0
Alkane-hydrochloride buffer；
Step 2: taking 7 10ml glass colorimetric cylinders, the 1st colorimetric cylinder of setting is blank tube, 2-7 root colorimetric cylinders are mark
Quasi- pipe, the zinc ion standard liquid of deionized water and various concentrations is added in blank tube and standard pipe, adds step respectively
Liquid-type zinc ion detection reagent described in one, reaction a period of time；
Step 3: the color as caused by each tube reaction in the bottom up shooting step 2 of colorimetric cylinder, uses mapping software
After each standard pipe color is deducted blank tube color by photoshop7.0, by the color of gained and corresponding zinc ion standard liquid
Concentration be printed upon on water white transparency PVC plastic flitch, obtain deduct reagent blank after zinc ion standard color card；
Step 4: separately taking 2 10ml glass colorimetric cylinders, the 1st colorimetric cylinder of setting is blank tube, the 2nd colorimetric cylinder is sample
QC, deionized water and water sample to be measured are added in blank tube and sample cell respectively, adds the liquid-type described in step 1
Zinc ion detection reagent, reaction a period of time；
Step 5: the blank bottom of the tube that the standard color card described in step 3 is placed in step 4, from colorimetric bottom of the tube to
It is upper observation blank tube be superimposed with standard color card after color, and with sample cell color comparator, when blank tube is superimposed with standard color card
When color afterwards is with sample cell solid colour, the zinc ion concentration corresponding to standard color card, as water are read within a certain period of time
Zinc ion concentration in sample.
Further, in step 1, described liquid-type zinc ion detection reagent is 100mmol/LpH8.0 trihydroxy methyl ammonia
Methylmethane-hydrochloride buffer, the concentration of the 2- contained (5- Bromo-2-pyridylazos) -5- diethylamino phenols is 0.10-0.30g/L,
TritonX-100 concentration is 0.1-0.3ml/L, the concentration of calgon 0.2-1.0g/L, sodium citrate is 1.0-2.0g/
L, the concentration of ethylene glycol is 30-50ml/L, and compound method is：Weigh 0.10-0.30g 2- (5- Bromo-2-pyridylazos) -5- two
Ethylamino phenol, 0.2-1.0g calgons, 1.0-2.0g sodium citrates, add 100mmol/L pH8.0 trihydroxy methyl amino first
Alkane-hydrochloride buffer stirring and dissolving, 0.1-0.3ml TritonX-100,30-50ml ethylene glycol are added, is used after stirring and dissolving
100mmol/L pH8.0 tris-HCI buffers are settled to 1000ml.
Further, in step 2, the concentration of described zinc ion standard liquid be respectively 0.04mg/L, 0.08mg/L,
0.12mg/L, 0.16mg/L, 0.20mg/L, 0.24mg/L, compound method are：It is 99.99% that precision, which weighs 0.1000g contents,
Metallic zinc, with 1000ml is settled to after 10ml 50% nitric acid dissolving, the zinc ion storing solution that concentration is 100mg/L is obtained,
With deionized water by zinc ion storing solution be diluted to concentration be 0.04mg/L, 0.08mg/L, 0.12mg/L, 0.16mg/L,
0.20mg/L, 0.24mg/L zinc ion standard liquid.
Further, in step 2 and step 4, the zinc ion standard liquid of described addition deionized water and various concentrations
Volume be 10ml.
Further, in step 2 and step 4, the volume of described addition liquid-type zinc ion detection reagent is 0.2ml.
Further, in step 2 and step 4, preferred reaction conditions are that 5-15 minutes are reacted in 5-40 DEG C of environment.
Further, in step 3, the thickness of described water white transparency PVC plastic flitch is 1-2mm.
Further, in step 5, described certain time is 5-10 minutes.
The zinc ion field fast detection method for eliminating reagent blank influence of the present invention has that method is simple, accuracy
The advantages of high, easily operated；Detected, can be effectively controlled needed for whole detection process using liquid-type zinc ion detection reagent
Time；Method by the way that blank tube color addition to be carried out to colorimetric control with sample cell again on standard color card, can effectively be eliminated
Influence of the reagent blank to testing result, is particularly suitable for zinc ion field quick detection in water.
Brief description of the drawings
Fig. 1 is zinc ion field fast detection method in the water provided in an embodiment of the present invention for eliminating reagent blank influence
Flow chart.
Embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, book is sent out
It is bright to be further elaborated.It should be appreciated that specific embodiment described herein is only to explain this present invention, not
For limiting the present invention.
Below in conjunction with the accompanying drawings and specific embodiment is further described to the application principle of the present invention.
As shown in figure 1, the present invention is achieved in that in a kind of water for eliminating reagent blank influence that zinc ion scene is fast
Fast detection method includes：
S101, liquid-type zinc ion detection reagent is prepared, the detection reagent is containing 2- (5- Bromo-2-pyridylazos) -5-
The trishydroxymethylaminomethane that diethylamino phenol, TritonX-100, calgon, sodium citrate, the pH of ethylene glycol are 8.0-
Hydrochloride buffer；
S102,7 10ml glass colorimetric cylinders are taken, the 1st colorimetric cylinder of setting is blank tube, 2-7 root colorimetric cylinders are standard
Pipe, the zinc ion standard liquid of deionized water and various concentrations is added in blank tube and standard pipe respectively, adds step
Liquid-type zinc ion detection reagent described in S101, reaction a period of time；
S103, the color as caused by each tube reaction in the bottom up shooting step S102 of colorimetric cylinder, use mapping software
After each standard pipe color is deducted blank tube color by photoshop7.0, by the color of gained and corresponding zinc ion standard liquid
Concentration be printed upon on water white transparency PVC plastic flitch, obtain deduct reagent blank after zinc ion standard color card；
S104,2 10ml glass colorimetric cylinders are separately taken, the 1st colorimetric cylinder of setting is blank tube, the 2nd colorimetric cylinder is sample
Pipe, deionized water and water sample to be measured are added in blank tube and sample cell respectively, adds the liquid-type described in step S101
Zinc ion detection reagent, reaction a period of time；
S105, the blank bottom of the tube that the standard color card described in step S103 is placed in step S104, by colorimetric bottom of the tube
Look up the color after blank tube is superimposed with standard color card, and with sample cell color comparator, when blank tube and reference colour card stack
When color after adding is with sample cell solid colour, the zinc ion concentration corresponding to standard color card is read within a certain period of time, is
Zinc ion concentration in water sample.
Further, in step S101, described liquid-type zinc ion detection reagent is 100mmol/LpH8.0 trihydroxy methyls
Aminomethane-hydrochloride buffer, the concentration of the 2- contained (5- Bromo-2-pyridylazos) -5- diethylamino phenols is 0.10-0.30g/
L, TritonX-100 concentration be 0.1-0.3ml/L, the concentration of calgon 0.2-1.0g/L, sodium citrate be 1.0-
2.0g/L, the concentration of ethylene glycol are 30-50ml/L, and compound method is：Weigh 0.10-0.30g 2- (5- Bromo-2-pyridylazos)-
5- diethylamino phenols, 0.2-1.0g calgons, 1.0-2.0g sodium citrates, add 100mmol/L pH8.0 trihydroxy methyl ammonia
Methylmethane-hydrochloride buffer stirring and dissolving, add 0.1-0.3ml TritonX-100,30-50ml ethylene glycol, stirring and dissolving
Afterwards 1000ml is settled to 100mmol/L pH8.0 tris-HCI buffers.
Further, in step S102, the concentration of described zinc ion standard liquid be respectively 0.04mg/L, 0.08mg/L,
0.12mg/L, 0.16mg/L, 0.20mg/L, 0.24mg/L, compound method are：It is 99.99% that precision, which weighs 0.1000g contents,
Metallic zinc, with 1000ml is settled to after 10ml 50% nitric acid dissolving, the zinc ion storing solution that concentration is 100mg/L is obtained,
With deionized water by zinc ion storing solution be diluted to concentration be 0.04mg/L, 0.08mg/L, 0.12mg/L, 0.16mg/L,
0.20mg/L, 0.24mg/L zinc ion standard liquid.
Further, in step S102 and step S104, the zinc ion standard of described addition deionized water and various concentrations
The volume of solution is 10ml.
Further, in step S102 and step S104, the volume of described addition liquid-type zinc ion detection reagent is
0.2ml。
Further, in step S102 and step S104, preferred reaction conditions are that 5-15 minutes are reacted in 5-40 DEG C of environment.
Further, in step S103, the thickness of described water white transparency PVC plastic flitch is 1-2mm.
Further, in step S105, described certain time is 5-10 minutes.
The specific embodiment of the present invention：
Embodiment 1
Step 1: weigh 0.10g 2- (5- Bromo-2-pyridylazos) -5- diethylamino phenols, 0.2g calgons, 1.0g
Sodium citrate, add 100mmol/L pH8.0 tris-HCI buffer stirring and dissolvings, add 0.1ml
TritonX-100,30ml ethylene glycol, 100mmol/L pH8.0 tris-HCI buffers are used after stirring and dissolving
1000ml is settled to, 1000ml is obtained and contains 0.10g/L2- (5- Bromo-2-pyridylazos) -5- diethylamino phenols, 0.1ml/L
TritonX-100,0.2g/L calgon, 1.0g/L sodium citrates, the hydroxyls of 100mmol/L pH8.0 tri- of 30ml/L ethylene glycol
Aminomethane-hydrochloride buffer is liquid-type zinc ion detection reagent；
Step 2: taking 7 10ml glass colorimetric cylinders, the 1st colorimetric cylinder of setting is blank tube, 2-7 root colorimetric cylinders are mark
Quasi- pipe, added respectively in blank tube and standard pipe 10ml deionized waters and 10ml concentration be 0.04mg/L, 0.08mg/L,
0.12mg/L, 0.16mg/L, 0.20mg/L, 0.24mg/L zinc ion standard liquid, add the liquid-type described in step 1
Zinc ion detection reagent 0.2ml, reacted 15 minutes in 5 DEG C of environment；
Step 3: the color as caused by each tube reaction in the bottom up shooting step 2 of colorimetric cylinder, uses mapping software
After each standard pipe color is deducted blank tube color by photoshop7.0, by the color of gained and corresponding zinc ion standard liquid
Concentration be printed upon thickness be 1mm water white transparency PVC plastic flitch on, obtain deduct reagent blank after each concentration zinc ion mark
Quasi- colour atla；
Step 4: separately taking 2 10ml glass colorimetric cylinders, the 1st colorimetric cylinder of setting is blank tube, the 2nd colorimetric cylinder is sample
QC, 10ml deionized waters and 10ml water samples to be measured are added in blank tube and sample cell respectively, is added described in step 1
Liquid-type zinc ion detection reagent, reacted 15 minutes in 5 DEG C of environment；
Step 5: the blank bottom of the tube that the standard color card described in step 3 is placed in step 4, from colorimetric bottom of the tube to
It is upper observation blank tube be superimposed with standard color card after color, and with sample cell color comparator, when both solid colours, at 5 points
The zinc ion concentration corresponding to standard color card is read in clock, show that zinc ion concentration is 0.04mg/L in water sample.
The zinc ion concentration of water sample described in the present embodiment step 4 is detected with national standard method atomic absorption method,
As a result it is 0.041mg/L, it can be seen that the present embodiment methods described has consistent well with the testing result of atomic absorption method
Property.
Embodiment 2
Step 1: weigh 0.20g 2- (5- Bromo-2-pyridylazos) -5- diethylamino phenols, 0.6g calgons, 1.5g
Sodium citrate, add 100mmol/L pH8.0 tris-HCI buffer stirring and dissolvings, add 0.2ml
TritonX-100,40ml ethylene glycol, 100mmol/L pH8.0 tris-HCI buffers are used after stirring and dissolving
1000ml is settled to, 1000ml is obtained and contains 0.20g/L2- (5- Bromo-2-pyridylazos) -5- diethylamino phenols, 0.2ml/L
TritonX-100,0.6g/L calgon, 1.5g/L sodium citrates, the hydroxyls of 100mmol/L pH8.0 tri- of 40ml/L ethylene glycol
Aminomethane-hydrochloride buffer is liquid-type zinc ion detection reagent；
Step 2: taking 7 10ml glass colorimetric cylinders, the 1st colorimetric cylinder of setting is blank tube, 2-7 root colorimetric cylinders are mark
Quasi- pipe, added respectively in blank tube and standard pipe 10ml deionized waters and 10ml concentration be 0.04mg/L, 0.08mg/L,
0.12mg/L, 0.16mg/L, 0.20mg/L, 0.24mg/L zinc ion standard liquid, add the liquid-type described in step 1
Zinc ion detection reagent 0.2ml, reacted 10 minutes in 20 DEG C of environment；
Step 3: the color as caused by each tube reaction in the bottom up shooting step 2 of colorimetric cylinder, uses mapping software
After each standard pipe color is deducted blank tube color by photoshop7.0, by the color of gained and corresponding zinc ion standard liquid
Concentration be printed upon thickness be 1.5mm water white transparency PVC plastic flitch on, obtain deduct reagent blank after each concentration zinc ion
Standard color card；
Step 4: separately taking 2 10ml glass colorimetric cylinders, the 1st colorimetric cylinder of setting is blank tube, the 2nd colorimetric cylinder is sample
QC, 10ml deionized waters and 10ml water samples to be measured are added in blank tube and sample cell respectively, is added described in step 1
Liquid-type zinc ion detection reagent, reacted 10 minutes in 20 DEG C of environment；
Step 5: the blank bottom of the tube that the standard color card described in step 3 is placed in step 4, from colorimetric bottom of the tube to
It is upper observation blank tube be superimposed with standard color card after color, and with sample cell color comparator, when both solid colours, 7.5
The zinc ion concentration corresponding to standard color card is read in minute, show that zinc ion concentration is 0.16mg/L in water sample.
The zinc ion concentration of water sample described in the present embodiment step 4 is detected with national standard method atomic absorption method,
As a result it is 0.165mg/L, it can be seen that the present embodiment methods described has consistent well with the testing result of atomic absorption method
Property.
Embodiment 3
Step 1: weigh 0.30g 2- (5- Bromo-2-pyridylazos) -5- diethylamino phenols, 1.0g calgons, 2.0g
Sodium citrate, add 100mmol/L pH8.0 tris-HCI buffer stirring and dissolvings, add 0.3ml
TritonX-100,50ml ethylene glycol, 100mmol/L pH8.0 tris-HCI buffers are used after stirring and dissolving
1000ml is settled to, 1000ml is obtained and contains 0.30g/L2- (5- Bromo-2-pyridylazos) -5- diethylamino phenols, 0.3ml/L
TritonX-100,1.0g/L calgon, 2.0g/L sodium citrates, the hydroxyls of 100mmol/L pH8.0 tri- of 50ml/L ethylene glycol
Aminomethane-hydrochloride buffer is liquid-type zinc ion detection reagent；
Step 2: taking 7 10ml glass colorimetric cylinders, the 1st colorimetric cylinder of setting is blank tube, 2-7 root colorimetric cylinders are mark
Quasi- pipe, added respectively in blank tube and standard pipe 10ml deionized waters and 10ml concentration be 0.04mg/L, 0.08mg/L,
0.12mg/L, 0.16mg/L, 0.20mg/L, 0.24mg/L zinc ion standard liquid, add the liquid-type described in step 1
Zinc ion detection reagent 0.2ml, reacted 5 minutes in 40 DEG C of environment；
Step 3: the color as caused by each tube reaction in the bottom up shooting step 2 of colorimetric cylinder, uses mapping software
After each standard pipe color is deducted blank tube color by photoshop7.0, by the color of gained and corresponding zinc ion standard liquid
Concentration be printed upon thickness be 2mm water white transparency PVC plastic flitch on, obtain deduct reagent blank after each concentration zinc ion mark
Quasi- colour atla；
Step 4: separately taking 2 10ml glass colorimetric cylinders, the 1st colorimetric cylinder of setting is blank tube, the 2nd colorimetric cylinder is sample
QC, 10ml deionized waters and 10ml water samples to be measured are added in blank tube and sample cell respectively, is added described in step 1
Liquid-type zinc ion detection reagent, reacted 5 minutes in 40 DEG C of environment；
Step 5: the blank bottom of the tube that the standard color card described in step 3 is placed in step 4, from colorimetric bottom of the tube to
It is upper observation blank tube be superimposed with standard color card after color, and with sample cell color comparator, when both solid colours, at 10 points
The zinc ion concentration corresponding to standard color card is read in clock, show that zinc ion concentration is 0.24mg/L in water sample.
The zinc ion concentration of water sample described in the present embodiment step 4 is detected with national standard method atomic absorption method,
As a result it is 0.236mg/L, it can be seen that the present embodiment methods described has consistent well with the testing result of atomic absorption method
Property.
Embodiment 4
In order to further prove the creativeness of the present invention, the present invention has done following contrast test, has investigated the change of reagent blank
Change the influence to measurement result accuracy.Method as described in the step 1 of this law embodiment 1 prepares the detection examination of liquid-type zinc ion
Agent is test group, not deduct reagent blank, use the detection reagent of standard color card direct color comparison as control group, by two group reagents
4 DEG C of refrigerators are put respectively and 37 water-baths store 7 days, and to being with aas determination zinc ion concentration with portion
0.04mg/L water sample is measured, while replaces water sample to carry out reagent blank test with deionized water, with 721 spectrophotometers
Reagent blank value is recorded, cuvette optical path is 1cm during test, and test wavelength 560nm, reference solution is deionized water, as a result
It is shown in Table 1.
Table 1
As it can be seen from table 1 under identical experiment condition, with the gradual increase of reagent blank, the measure of test group
As a result keep constant, but the measurement result of control group substantially becomes big, shows that method of the present invention can effectively remove reagent sky
Influence of the white change to zinc ion test result.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
All any modification, equivalent and improvement made within refreshing and principle etc., should be included in the scope of the protection.
Claims (10)
1. zinc ion field fast detection method in a kind of water for eliminating reagent blank influence, it is characterised in that described can
Eliminating zinc ion field fast detection method in the water that reagent blank influences includes：
Step 1: preparing liquid-type zinc ion detection reagent, the detection reagent is containing 2- (5- Bromo-2-pyridylazos) -5- two
Trishydroxymethylaminomethane-salt that ethylamino phenol, TritonX-100, calgon, sodium citrate, the pH of ethylene glycol are 8.0
Acid buffer；
Step 2: taking 7 10ml glass colorimetric cylinders, the 1st colorimetric cylinder of setting is blank tube, 2-7 root colorimetric cylinders are standard pipe,
The zinc ion standard liquid of deionized water and various concentrations is added in blank tube and standard pipe respectively, adds the liquid of step 1
Build zinc ion detection reagent, is reacted；
Step 3: the color as caused by each tube reaction in the bottom up shooting step 2 of colorimetric cylinder, uses mapping software
After each standard pipe color is deducted blank tube color by photoshop7.0, by the color of gained and corresponding zinc ion standard liquid
Concentration be printed upon on water white transparency PVC plastic flitch, obtain deduct reagent blank after zinc ion standard color card；
Step 4: separately taking 2 10ml glass colorimetric cylinders, the 1st colorimetric cylinder of setting is blank tube, the 2nd colorimetric cylinder is sample cell,
Deionized water and water sample to be measured are added in blank tube and sample cell respectively, adds the liquid-type zinc ion described in step 1
Detection reagent, reacted；
Step 5: the blank bottom of the tube that the standard color card described in step 3 is placed in step 4, by the bottom up sight of colorimetric cylinder
Examine the color after blank tube is superimposed with standard color card, and with sample cell color comparator, after blank tube is superimposed with standard color card
When color is with sample cell solid colour, the zinc ion concentration corresponding to standard color card, as zinc ion concentration in water sample are read.
2. zinc ion field fast detection method in the water of reagent blank influence, its feature can be eliminated as claimed in claim 1
It is, in step 1, described liquid-type zinc ion detection reagent is 100mmol/LpH8.0 trishydroxymethylaminomethanes-hydrochloric acid
Buffer solution, the concentration of the 2- contained (5- Bromo-2-pyridylazos) -5- diethylamino phenols is 0.10-0.30g/L, TritonX-100
Concentration be 0.1-0.3ml/L, the concentration of calgon 0.2-1.0g/L, sodium citrate is 1.0-2.0g/L, ethylene glycol
Concentration is 30-50ml/L.
3. zinc ion field fast detection method in the water of reagent blank influence, its feature can be eliminated as claimed in claim 2
It is, tris-HCI buffer compound method is：Weigh 0.10-0.30g2- (5- Bromo-2-pyridylazos)-
5- diethylamino phenols, 0.2-1.0g calgons, 1.0-2.0g sodium citrates, add 100mmol/L pH8.0 trihydroxy methyl ammonia
Methylmethane-hydrochloride buffer stirring and dissolving, add 0.1-0.3ml TritonX-100,30-50ml ethylene glycol, stirring and dissolving
Afterwards 1000ml is settled to 100mmol/L pH8.0 tris-HCI buffers.
4. zinc ion field fast detection method in the water of reagent blank influence, its feature can be eliminated as claimed in claim 1
Be, in step 2, the concentration of described zinc ion standard liquid be respectively 0.04mg/L, 0.08mg/L, 0.12mg/L,
0.16mg/L、0.20mg/L、0.24mg/L。
5. zinc ion field fast detection method in the water of reagent blank influence, its feature can be eliminated as claimed in claim 4
It is, zinc ion standard liquid compound method is：The metallic zinc that 0.1000g contents are 99.99% is weighed, with 10ml's 50%
1000ml is settled to after nitric acid dissolving, concentration is obtained and is 100mg/L zinc ion storing solution, then stored up zinc ion with deionized water
Standby liquid is diluted to the zinc ion that concentration is 0.04mg/L, 0.08mg/L, 0.12mg/L, 0.16mg/L, 0.20mg/L, 0.24mg/L
Standard liquid.
6. zinc ion field fast detection method in the water of reagent blank influence, its feature can be eliminated as claimed in claim 1
It is, in step 2, the zinc ion standard liquid volumes of described addition deionized water and various concentrations is 10ml, step 4
In, the volume of described addition deionized water is 10ml.
7. zinc ion field fast detection method in the water of reagent blank influence, its feature can be eliminated as claimed in claim 1
It is, in step 2 and step 4, the volume of described addition liquid-type zinc ion detection reagent is 0.2ml.
8. zinc ion field fast detection method in the water of reagent blank influence, its feature can be eliminated as claimed in claim 1
It is, in step 2 and step 4, reaction condition is that 5-15 minutes are reacted in 5-40 DEG C of environment.
9. zinc ion field fast detection method in the water of reagent blank influence, its feature can be eliminated as claimed in claim 1
It is, in step 3, the thickness of described water white transparency PVC plastic flitch is 1-2mm.
10. zinc ion field fast detection method in the water of reagent blank influence, its feature can be eliminated as claimed in claim 1
It is, in step 5, reaction 5-10 minutes read the zinc ion concentration corresponding to standard color card.
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