CN105273831A - Wall-breaking and surfactant-assisted method used for extracting grease from microalgae containing oil - Google Patents
Wall-breaking and surfactant-assisted method used for extracting grease from microalgae containing oil Download PDFInfo
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Abstract
The invention discloses a wall-breaking and surfactant-assisted method used for extracting grease from microalgae containing oil. The wall-breaking and surfactant-assisted method comprises following steps: (1) pH value of a suspension liquid of microalgae containing oil is adjusted to be 7.5 to 14, and is subjected to hydro-thermal treatment; (2) after hydro-thermal treatment, enzymatic hydrolysis is carried out in the presence of enzymes; and (3) after enzymatic hydrolysis, a surfactant is added for reaction, and an organic solvent is added for extraction and separation of grease. Grease extraction rate of the wall-breaking and surfactant-assisted method is high; operation conditions are mild; nutritional ingredients of microalgae can be maintained effectively; using amount of the organic solvent is small; the wall-breaking and surfactant-assisted method is friendly to the environment, is low in energy consumption, and is suitable for nannochloropsis oculata, chlorella, diatom, scenedesmus, and monoraphidium komarkova-legnerova; and application range is wide.
Description
Technical field
The present invention relates to micro-algae technical field of bioengineering, particularly, relate to a kind of broken wall of oil-containing micro-algae and the method for tensio-active agent assisted extraction grease.
Background technology
Micro-algae refers to the small algae colony that just can distinguish its form under the microscope, is most important primary productivity in biosphere.It is large that algae has biomass, and the advantages such as with short production cycle, of a great variety, oleaginousness is high, and adaptive capacity to environment is strong are the very good materials of biofuel.In addition also containing the compositions such as multivitamin, carotene, protein, lipid acid in micro-algae, is the source of active medicinal matter.
But microalgae grease is all wrapped in cell, the biologically active substance of micro-algae is as protein in addition, carotenoid, and the great majority such as microalgal polysaccharide are all present in biomass cells.Due to the less and tool cell wall structure of microalgae cell, the microalgae cell of most of kind has cellulosic cell walls, the microalgae cell wall of some kind is difficult to other composition of digest and decompose in addition in a large number, cell wall thickness, quality is hard, be unfavorable for the extraction of grease, also greatly reduce the bioavailability of micro-algae product.Therefore the broken wall of micro-algae and the extraction of grease are the keys utilizing grease in micro-algae and other useful component.
The extraction of the grease of oil-containing micro-algae is the gordian technique of biological diesel oil refining always.Usually hydrothermal method is adopted at present.Hydrothermal method normally carries out cell wall breaking, grease stripping by High Temperature High Pressure in the basic conditions, general temperature is 100-300 DEG C, energy consumption is high, the molten slurry of gained oil-containing micro-algae carries out oily water separation by centrifugal, as CN100460513C, but due to the molten slurry of the oleaginous microorganism obtained be the milk sap containing microorganism slag, oil phase and aqueous phase mix, be difficult to be separated, undertaken that oily water separation energy consumption is higher, grease extraction efficiency is lower by centrifugal.
At present, the method for fracturing cell walls has polishing, multigelation method and ultrasonication method etc.Wherein polishing complex operation, and the easy intracellular original liquid component of collateral damage, cytoclasis degree is lower, can not realize mass-producing.The problems such as multigelation method exists can not realize mass-producing, and running cost is high, and energy consumption is high.Ultrasonication method can destroy other active ingredient intracellular, and running cost is higher.
In addition, the extraction of microalgae grease is the gordian technique of micro-algae energy.Common Microalgae grease extraction method has supercritical carbon dioxide extraction method, soxhlet extraction methods, the methods such as organic solvent extraction.Supercritical carbon dioxide extraction method complicated operation, apparatus expensive, severe reaction conditions, not easily realizes mass-producing.It is large to there is organic solvent usage quantity in soxhlet extraction methods and organic solvent extraction, and toxicity is large, pollutes large, the shortcoming that oil extracting rate is not high.
CN102517146B discloses a kind of method that aqueous extraction algae oil assisted by tensio-active agent, under the condition forming ultra low interfacial tension and critical micro emulsion concentration, broken for excusing from death is split kettle frustule and contains novel gemini surfactants C
10h
21-18PO-2EO-SO
4the salt brine solution mixing of Na, under the condition of 20-25 DEG C, with certain tachyphylaxis regular hour, carries out centrifugation by the mixed solution obtained after reaction terminates, and upper strata is free lipid layer, and middle level is surfactant layer, and lower floor is water layer.The shortcoming of the method utilizes ultrasonication, and destroy other active ingredient intracellular, running cost is higher, and the method is only for splitting kettle algae, not for other algae, and narrow application range.
Therefore, need to explore a kind of grease extraction efficiency high, effectively can keep that the nutrition composition of micro-algae, operational condition are gentle, consumption of organic solvent is few, oil-containing micro-algae broken wall that environmental protection, energy consumption are low, applied widely and the method that grease extracts.
Summary of the invention
The object of the invention is to overcome existing oil-containing micro-algae broken wall and grease extract in original liquid component, severe reaction conditions in low, the destructible cell of grease extraction efficiency, consumption of organic solvent is large, environmental pollution is serious, energy consumption is high or the shortcoming such as narrow application range, provides a kind of method that broken wall of new oil-containing micro-algae and grease thereof extract.
The present inventor finds under study for action, oil-containing micro-algae is carried out hydrothermal treatment consists under alkaline condition and lower temperature condition and in conjunction with enzyme digestion reaction together broken wall, then, under the effect of tensio-active agent, a small amount of organic solvent is utilized can to realize the extraction of microalgae grease.The grease extraction efficiency of the method is high, operational condition is gentle, effectively can keep that the nutrition composition of micro-algae, consumption of organic solvent are few, environmental protection, energy consumption are low, and multiple micro-algaes such as micro-plan ball algae, chlorella, diatom, grid algae and single needle algae can be applicable to, applied widely.
Therefore, to achieve these goals, the invention provides a kind of broken wall of oil-containing micro-algae and the method for tensio-active agent assisted extraction grease, described method comprises the steps:
(1) be 7.5-14 by the suspension adjust ph of oil-containing micro-algae, carry out hydrothermal treatment consists;
(2), after hydrothermal treatment consists, under enzyme exists, enzyme digestion reaction is carried out;
(3) add tensio-active agent after enzyme digestion reaction to react, then add organic solvent and carry out extracting and isolating grease.
Preferably, the condition of hydrothermal treatment consists comprises: temperature is 50-120 DEG C, and the time is 0.5-10h.
Preferably, described tensio-active agent is Anionic-nonionic type tensio-active agent and/or nonionogenic tenside; More preferably, described Anionic-nonionic type tensio-active agent is
described nonionogenic tenside is
still more preferably, described tensio-active agent is
with
and
with
mol ratio be 1:4-6.
Preferably, relative to the suspension of described oil-containing micro-algae, the consumption of described tensio-active agent is 20-400mg/L.
Preferably, with the dry weight basis of described oil-containing micro-algae, relative to oil-containing micro-algae described in 1g, the consumption of described organic solvent is 1-30mL.
The broken wall of oil-containing micro-algae of the present invention and the method for tensio-active agent assisted extraction grease, owing to adopting hydrothermal treatment consists and enzyme digestion reaction combined wall breakage, hydrothermal treatment consists can be carried out under relatively mild condition, avoid High Temperature High Pressure, be conducive to the maintenance of the biological property of each nutrition unit in micro-algae, reduce cost, improve shell-broken effect; Owing to adding tensio-active agent, contribute to the precipitation of microalgae grease, greatly reduce the consumption of organic solvent, reduce further cost, and decrease the pollution to environment; The grease extraction efficiency of the inventive method can up to more than 80%, and grease extraction efficiency is high; The inventive method is simple to operate, and can be applicable to multiple micro-algaes such as micro-plan ball algae, chlorella, diatom, grid algae and single needle algae, applied widely, is easy to realize mass-producing.
Other features and advantages of the present invention are described in detail in embodiment part subsequently.
Embodiment
Below the specific embodiment of the present invention is described in detail.Should be understood that, embodiment described herein, only for instruction and explanation of the present invention, is not limited to the present invention.
The invention provides a kind of broken wall of oil-containing micro-algae and the method for tensio-active agent assisted extraction grease, the method comprises the steps:
(1) be 7.5-14 by the suspension adjust ph of oil-containing micro-algae, carry out hydrothermal treatment consists;
(2), after hydrothermal treatment consists, under enzyme exists, enzyme digestion reaction is carried out;
(3) add tensio-active agent after enzyme digestion reaction to react, then add organic solvent and carry out extracting and isolating grease.
In the present invention, oil-containing micro-algae refers to from oil-containing micro-algae nutrient solution, collect the oil-containing micro-algae obtained slurries or its algae powder through super-dry.The inventive method is applicable to various oil-containing micro-algae, namely the kind of oil-containing micro-algae is had no particular limits, preferably have the produce oil engineering microalgae of larger industrial utilization, such as, oil-containing micro-algae can be selected from least one in micro-plan ball algae, chlorella, diatom, single needle algae and grid algae.
In step of the present invention (1), for the concentration of the suspension of oil-containing micro-algae, with the dry weight basis of oil-containing micro-algae, the content of oil-containing micro-algae in suspension is preferably 2-30 % by weight, is more preferably 15-30 % by weight.
In step of the present invention (1), suspension to the pH value regulating oil-containing micro-algae is 7.5-14, be preferably 8.5-12, for pH value condition, alkali can be added regulate, for alkali without particular requirement, the alkali that this area is conventional can be adopted, such as, alkali can be selected from one or more in oxyhydroxide, carbonate, supercarbonate and phosphoric acid salt.
In the present invention, pH value is recorded by pH determinator.
In the present invention, hydrothermal condition refers to and keeps liquid condition at a certain temperature.The inventive method carries out broken wall owing to adopting hydrothermal treatment consists and enzyme digestion reaction to combine, therefore, hydrothermal treatment consists can be carried out under relatively mild condition, in step of the present invention (1), the condition optimization of hydrothermal treatment consists comprises: temperature is 50-120 DEG C, be more preferably 60-100 DEG C, the time is 0.5-10h, is more preferably 2-6h.It will be understood by those skilled in the art that hydrothermal treatment consists is preferably under agitation carried out.
Temperature 100-300 DEG C of the hydrothermal treatment consists that the temperature of the inventive method hydrothermal treatment consists is more conventional greatly can be reduced energy consumption, and be conducive to the maintenance of the biological property of each nutrition unit in micro-algae.
In step of the present invention (2), carry out the various enzymes that enzyme that enzyme digestion reaction uses can use for this area routine, such as can be selected from least one in cellulase, polygalacturonase, proteolytic enzyme, N,O-Diacetylmuramidase and helicase, be preferably cellulase, polygalacturonase, proteolytic enzyme and N,O-Diacetylmuramidase.These enzymes all can by commercially available.
In step of the present invention (2), for the consumption of enzyme, with the dry weight basis of oil-containing micro-algae, the consumption of enzyme is preferably 50-5000IU/g, is more preferably 200-1200IU/g.When enzyme is the combination of several enzyme, for the consumption of various enzyme without particular requirement, as long as the preferred above-mentioned scope of total amount.
In step of the present invention (2), the condition optimization of enzyme digestion reaction comprises: temperature is 30-70 DEG C, is more preferably 40-60 DEG C; PH value is 3-7, is more preferably 4-5.5; Time, with till the condition that can meet complete crushing microalgae cells, is 2-10h, is more preferably 4-6h.For pH value condition, can acid adding regulate, for acid without particular requirement, can adopt the acid that this area is conventional, can be mineral acid, also can be organic acid, and such as, acid can be acetic acid, citric acid.
In step of the present invention (3), tensio-active agent is preferably Anionic-nonionic type tensio-active agent and/or nonionogenic tenside.Anionic-nonionic type tensio-active agent is preferably
nonionogenic tenside is preferably
The present inventor surprisingly finds under study for action, when tensio-active agent is
with
combination, and
with
mol ratio when being 1:4-6, greatly can improve the grease extraction efficiency of oil-containing micro-algae, therefore, more preferably, tensio-active agent is
with
combination, and
with
mol ratio be 1:4-6.
The principle of tensio-active agent assisted extraction grease is: the ester group of grease molecules can be adsorbed on (as polypeptide, glycolipid etc.) in the remnant tissue after microalgae wall breaking by interaction of hydrogen bond, surfactant molecule produces stronger electrostatic interaction by hydrophilic head base and these remnant tissues, can adsorb with competing property of grease molecules, grease molecules is replaced; Grease molecules is because its hydrophobic property, in water, there is high interfacial energy and tend to be adsorbed in the cell tissue of relative hydrophobic, surfactant molecule can form aggregate in aqueous and produce hydrophobic microcell, strong increase-volume, dispersive ability is had to grease molecules, reduce oil water interfacial tension, grease molecules is entered in the aqueous solution from cell tissue transfer.
In the present invention, relative to the suspension of oil-containing micro-algae, the consumption of tensio-active agent is preferably 20-400mg/L, is more preferably 100-400mg/L.
In step of the present invention (3), add the condition optimization that tensio-active agent carries out reacting and comprise: temperature is 20-80 DEG C, is more preferably 40-60 DEG C, and the time is 1-3h, is more preferably 1.5-2.5h.What those skilled in the art should understand that is, play a role better to make tensio-active agent, add tensio-active agent when reacting, the modes such as preferred employing vibration, stirring make tensio-active agent fully contact with algae liquid, to make the grease molecules of oil-containing micro-algae enter better in the aqueous solution, with the extraction of more convenient organic solvent.
In the present invention, the various organic solvents that organic solvent can be commonly used for this area, are preferably trichloromethane, normal hexane, sherwood oil.
In the inventive method, owing to adding tensio-active agent, grease molecules is more easily by organic solvent extraction, therefore, greatly reduce the consumption of organic solvent, with the dry weight basis of oil-containing micro-algae, relative to 1g oil-containing micro-algae, the consumption of organic solvent is preferably 1-30mL, is more preferably 2-10mL.
And, the present inventor finds under study for action, the grease in micro-algae is extracted in prior art, what adopt is the mode that polar organic solvent and non-polar organic solvent share, adopt the inventive method, add tensio-active agent, only extract just can reach with non-polar organic solvent and share the better extraction effect of extraction than prior art Semi-polarity organic solvent and non-polar organic solvent, therefore, further illustrate the inventive method and greatly can reduce consumption of organic solvent.
What those skilled in the art should understand that is, in order to make organic solvent oil and grease extracting molecule better, when adding organic solvent and extracting, preferably adopt the modes such as stirring, vibration, ultrasonic wave that organic solvent is fully contacted with algae liquid, the time of contact is generally 25-35min.
In the inventive method, add after organic solvent extracts, grease molecules enters in organic solvent, isolates the organic solvent containing grease, can adopt the various methods that this area is conventional, such as centrifugal, stratification etc., preferably centrifugal.For centrifugal actual conditions without particular requirement, the normal condition of this area can be adopted, such as, can under 4000-6000rpm centrifugal 5-15min.
After being separated the organic solvent obtained containing grease, it will be understood by those skilled in the art that and also need organic solvent to remove, to obtain purer microalgae grease.The method removing organic solvent can be the thinkable various method of those skilled in the art, such as volatilization, distillation, underpressure distillation etc. naturally, is preferably underpressure distillation.
It will be understood by those skilled in the art that the consumption in order to reduce organic solvent further, in actual production, removing again containing the organic solvent in the organic solvent of grease after organic solvent can being recycled several times.
Embodiment
The present invention is further illustrated for following embodiment, but therefore do not limit the present invention.
In following embodiment and comparative example:
The weight of weight/micro-algae dry powder of the grease of grease extraction efficiency=extraction.
PH value is recorded by pH determinator (Mettler-Toledo Instrument (Shanghai) Co., Ltd., SG23).
Stirring velocity is 30rpm.
Embodiment 1
The broken wall of the present embodiment for illustration of oil-containing micro-algae of the present invention and the method for tensio-active agent assisted extraction grease.
(1) micro-for 5g plan ball algae dry powder is put into triangular flask, add water be mixed with 25 % by weight suspension, and adjust ph is to 8.5, at 80 DEG C, stir 4h;
(2) regulate pH value to 5, in micro-plan ball algae dry powder, (Beijing letter profit is precious along Science and Technology Ltd. all to add cellulase according to the amount of 100IU/g, 2000IU/g, lower with), (Beijing letter profit is precious along Science and Technology Ltd. for polygalacturonase, 20000IU/g, lower with), (Beijing letter profit is precious along Science and Technology Ltd. for aspartic protease, 50000IU/g, lower same) and N,O-Diacetylmuramidase (Chemical Reagent Co., Ltd., Sinopharm Group, 20000IU/g, lower same), at 50 DEG C, react 5h;
(3) relative to the suspension of micro-plan ball algae, add according to the amount of 200mg/L
with
with
mol ratio be 1:5, stirring reaction 2h at 50 DEG C, then add 20ml normal hexane, stirs 30min, centrifugal 10min under 5000rpm, the organic solvent layer containing grease above getting, then 100-200Pa, underpressure distillation at 68 DEG C, constant weight is dry, and obtain grease, the extraction yield of grease is in table 1.
Embodiment 2
The broken wall of the present embodiment for illustration of oil-containing micro-algae of the present invention and the method for tensio-active agent assisted extraction grease.
(1) 5g chlorella dry powder is put into triangular flask, add water be mixed with 15 % by weight suspension, and adjust ph is to 10, at 60 DEG C, stir 6h;
(2) regulate pH value to 4, in chlorella dry powder, all add cellulase, polygalacturonase, aspartic protease and N,O-Diacetylmuramidase according to the amount of 200IU/g, at 40 DEG C, react 6h;
(3) relative to the suspension of chlorella, add according to the amount of 100mg/L
with
and
with
mol ratio be 1:4, stirring reaction 2.5h at 40 DEG C, then add 10ml trichloromethane, stirs 25min, centrifugal 15min under 4000rpm, the organic solvent layer containing grease above getting, then 100-200Pa, underpressure distillation at 60 DEG C, constant weight is dry, and obtain grease, the extraction yield of grease is in table 1.
Embodiment 3
The broken wall of the present embodiment for illustration of oil-containing micro-algae of the present invention and the method for tensio-active agent assisted extraction grease.
(1) 5g grid algae dry powder is put into triangular flask, add water be mixed with 30 % by weight suspension, and adjust ph is to 12, at 100 DEG C, stir 2h;
(2) regulate pH value to 5.5, in grid algae dry powder, all add cellulase, polygalacturonase, aspartic protease and N,O-Diacetylmuramidase according to the amount of 300IU/g, at 60 DEG C, react 4h;
(3) relative to the suspension of grid algae, add according to the amount of 400mg/L
with
with
mol ratio be 1:6, stirring reaction 1.5h at 60 DEG C, then add 50ml sherwood oil (Beijing Chemical Plant, analytical pure), stir 35min, centrifugal 5min under 6000rpm, the organic solvent layer containing grease above getting, then 100-200Pa, underpressure distillation at 50 DEG C, constant weight is dry, and obtain grease, the extraction yield of grease is in table 1.
Embodiment 4
The broken wall of the present embodiment for illustration of oil-containing micro-algae of the present invention and the method for tensio-active agent assisted extraction grease.
The broken wall of oil-containing micro-algae and the extraction of grease is carried out according to the method for embodiment 1, unlike, in step (3), tensio-active agent only adopts
the extraction yield of grease is in table 1.
Embodiment 5
The broken wall of the present embodiment for illustration of oil-containing micro-algae of the present invention and the method for tensio-active agent assisted extraction grease.
The broken wall of oil-containing micro-algae and the extraction of grease is carried out according to the method for embodiment 1, unlike, in step (3), tensio-active agent only adopts
the extraction yield of grease is in table 1.
Embodiment 6
The broken wall of the present embodiment for illustration of oil-containing micro-algae of the present invention and the method for tensio-active agent assisted extraction grease.
The broken wall of oil-containing micro-algae and the extraction of grease is carried out according to the method for embodiment 1, unlike, in step (3), relative to the suspension of micro-plan ball algae, add tensio-active agent according to the amount of 20mg/L.The extraction yield of grease is in table 1.
Comparative example 1
The broken wall of oil-containing micro-algae and the extraction of grease is carried out according to the method for embodiment 1, unlike, in step (3), do not add tensio-active agent, directly carry out the extraction of organic solvent.The extraction yield of grease is in table 1.
Comparative example 2
The broken wall of oil-containing micro-algae and the extraction of grease is carried out according to the method for embodiment 1, unlike, save step (2).The extraction yield of grease is in table 1.
Table 1
| Sequence number | The extraction yield of grease |
| Embodiment 1 | 94.3 |
| Embodiment 2 | 95.9 |
| Embodiment 3 | 96.7 |
| Embodiment 4 | 85.9 |
| Embodiment 5 | 86.1 |
| Embodiment 6 | 84.3 |
| Comparative example 1 | 63.2 |
| Comparative example 2 | 38.9 |
Embodiment 1-6 and comparative example 1-2 is compared and can be found out, the inventive method adopts hydrothermal treatment consists broken wall together with enzyme digestion reaction, and then under the effect of tensio-active agent, utilize the grease in organic solvent extraction oil-containing micro-algae, extraction yield is high.
Embodiment 1 is compared can be found out with embodiment 4 and embodiment 5 respectively, and tensio-active agent is
with
combination, and
with
mol ratio when being 1:4-6, greatly can improve the grease extraction efficiency of oil-containing micro-algae further; Embodiment 1 and embodiment 6 are compared and can be found out, relative to the suspension of oil-containing micro-algae, the consumption of tensio-active agent is 100-400mg/L, can improve the grease extraction efficiency of oil-containing micro-algae further.
The broken wall of oil-containing micro-algae of the present invention and the method for tensio-active agent assisted extraction grease, owing to adopting hydrothermal treatment consists and enzyme digestion reaction combined wall breakage, hydrothermal treatment consists can be carried out under relatively mild condition, avoid High Temperature High Pressure, be conducive to the maintenance of the biological property of each nutrition unit in micro-algae, reduce cost, improve shell-broken effect; Owing to adding tensio-active agent, contribute to the precipitation of microalgae grease, greatly reduce the consumption of organic solvent, reduce further cost, and decrease the pollution to environment; The grease extraction efficiency of the inventive method can up to more than 80%, and grease extraction efficiency is high; The inventive method is simple to operate, and can be applicable to multiple micro-algaes such as micro-plan ball algae, chlorella, diatom, grid algae and single needle algae, applied widely, is easy to realize mass-producing.
More than describe the preferred embodiment of the present invention in detail; but the present invention is not limited to the detail in above-mentioned embodiment, within the scope of technical conceive of the present invention; can carry out multiple simple variant to technical scheme of the present invention, these simple variant all belong to protection scope of the present invention.
It should be noted that in addition, each concrete technical characteristic described in above-mentioned embodiment, in reconcilable situation, can be combined by any suitable mode, in order to avoid unnecessary repetition, the present invention illustrates no longer separately to various possible array mode.
In addition, also can carry out arbitrary combination between various different embodiment of the present invention, as long as it is without prejudice to thought of the present invention, it should be considered as content disclosed in this invention equally.
Claims (14)
1. the broken wall of oil-containing micro-algae and a method for tensio-active agent assisted extraction grease, it is characterized in that, described method comprises the steps:
(1) be 7.5-14 by the suspension adjust ph of oil-containing micro-algae, carry out hydrothermal treatment consists;
(2), after hydrothermal treatment consists, under enzyme exists, enzyme digestion reaction is carried out;
(3) add tensio-active agent after enzyme digestion reaction to react, then add organic solvent and carry out extracting and isolating grease.
2. method according to claim 1, wherein, in step (1), with the dry weight basis of described oil-containing micro-algae, the content of described oil-containing micro-algae in described suspension is 2-30 % by weight.
3. method according to claim 1, wherein, in step (1), the condition of hydrothermal treatment consists comprises: temperature is 50-120 DEG C, and the time is 0.5-10h.
4. method according to claim 1, wherein, described enzyme is selected from least one in cellulase, polygalacturonase, proteolytic enzyme, N,O-Diacetylmuramidase and helicase.
5. method according to claim 4, wherein, with the dry weight basis of described oil-containing micro-algae, the consumption of enzyme is 50-5000IU/g, is preferably 200-1200IU/g.
6. method according to claim 1, wherein, in step (2), the condition of described enzyme digestion reaction comprises: temperature is 30-70 DEG C, is preferably 40-60 DEG C; PH value is 3-7, is preferably 4-5.5; Time is 2-10h.
7. method according to claim 1, wherein, described tensio-active agent is Anionic-nonionic type tensio-active agent and/or nonionogenic tenside.
8. method according to claim 7, wherein, described Anionic-nonionic type tensio-active agent is
described nonionogenic tenside is
9. method according to claim 8, wherein, described tensio-active agent is
with
and
with
mol ratio be 1:4-6.
10., according to the method in claim 7-9 described in any one, wherein, relative to the suspension of described oil-containing micro-algae, the consumption of described tensio-active agent is 20-400mg/L.
11. methods according to claim 1, wherein, in step (3), add the condition that tensio-active agent carries out reacting and comprise: temperature is 20-80 DEG C, and the time is 1-3h.
12. methods according to claim 1, wherein, described organic solvent is selected from trichloromethane, normal hexane, sherwood oil.
13. methods according to claim 1, wherein, with the dry weight basis of described oil-containing micro-algae, relative to oil-containing micro-algae described in 1g, the consumption of described organic solvent is 1-30mL.
14. methods according to claim 1, wherein, described oil-containing micro-algae is at least one in micro-plan ball algae, chlorella, diatom, single needle algae and grid algae.
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| CN118028051A (en) * | 2024-04-11 | 2024-05-14 | 山东悦翔生物有限公司 | Method for extracting grease from microalgae |
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| CN107916191A (en) * | 2017-12-29 | 2018-04-17 | 烟台智祺化学技术有限公司 | A kind of production method for not adding sulfur dioxide organic wine |
| CN108624403A (en) * | 2018-04-28 | 2018-10-09 | 武汉工程大学 | A kind of method of low temperature non-organic solvent extraction energy insect oils |
| CN109734264A (en) * | 2018-11-20 | 2019-05-10 | 江南大学 | A method of promoting the release of bloom blue algae content |
| CN114292702A (en) * | 2021-12-31 | 2022-04-08 | 河南省商业科学研究所有限责任公司 | Method for extracting peony essential oil by using surfactant aqueous solution and solid alkali |
| CN114292702B (en) * | 2021-12-31 | 2023-06-16 | 河南省商业科学研究所有限责任公司 | Method for extracting peony essential oil by using surfactant aqueous solution and solid alkali |
| CN118028051A (en) * | 2024-04-11 | 2024-05-14 | 山东悦翔生物有限公司 | Method for extracting grease from microalgae |
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