CN105273068B - Colony induction signaling peptide AIP and its application - Google Patents
Colony induction signaling peptide AIP and its application Download PDFInfo
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- CN105273068B CN105273068B CN201510756056.4A CN201510756056A CN105273068B CN 105273068 B CN105273068 B CN 105273068B CN 201510756056 A CN201510756056 A CN 201510756056A CN 105273068 B CN105273068 B CN 105273068B
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
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- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/13—Fermented milk preparations; Treatment using microorganisms or enzymes using additives
- A23C9/1322—Inorganic compounds; Minerals, including organic salts thereof, oligo-elements; Amino-acids, peptides, protein-hydrolysates or derivatives; Nucleic acids or derivatives; Yeast extract or autolysate; Vitamins; Antibiotics; Bacteriocins
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Abstract
The invention discloses colony induction signaling peptide AIP and its applications.The present invention protects polypeptide I first, as shown in the sequence 8 of sequence table.The present invention also protects polypeptide II, for the polypeptide of amino acid shown in the sequence 8 containing ordered list.The present invention also protects a kind of preparation method of sour milk product, includes the following steps：Lactobacillus ljj 6 is seeded to liquid milk, is then fermented, polypeptide I or polypeptide II is then added, obtains sour milk product.The present invention has theory value for the research of quorum sensing phenomenon, and prepared by Yoghourt have great application value.
Description
Technical field
The present invention relates to colony induction signaling peptide AIP and its applications.
Background technology
Quorum sensing (Quorum Sensing, QS) refers to the expression of its gene of certain micro-organisms group by close with group
The phenomenon that spending relevant signaling molecule regulation and control.QS systems participate in a series of biological functions, such as：Thalline shine, antibiotic
Biosynthesis, the generation of virulence factor, the synthesis of exocellular polysaccharide, bacterium gather together, biofilm formation, plasmid engagement transfer etc..
QS systems are considered participating in the multinomial physiological function of regulation and control bacterium, which includes self-induction peptide
(autoinducingpeptide, AIP), Protein histidine kinase and induction regulatory protein, also known as three-component system.AIP makees
For signaling molecule, it is used to indicate cell density, when AIP reaches a certain critical concentration threshold, can be activated a series of specific
The expression of gene.The current presence that quorum sensing phenomenon has been observed in various bacteria, such as staphylococcus aureus,
Streptococcus mutans etc..
Some bacteriums of nature have intervention school-based, can be by monitoring colony induction signaling point in ambient enviroment
Sub- concentration, to perceive the quantity variation of other floras in itself and ambient enviroment, when signaling molecule concentration reaches certain threshold value
Just start thalline related gene expression, regulates and controls bacteria concentration and related biological function.
Yoghourt is the dairy products containing viable bacteria, after normal fermentation, product storage, transport, sale, it is edible before this mistake
Cheng Zhong, thalline are still to growth and breeding, and acidification phenomenon (postacidification) after generation, the i.e. pH value of Yoghourt continues to decline,
Down to occurring, consumer is unacceptable to cross tart flavour and aesthetic quality's decline.Caused by post-acidification of yoghurt phenomenon the result is that Yoghourt without
Method is stored for a long time, and Yoghourt storage period short defect annoyings the consumption of Yoghourt.
Invention content
The object of the present invention is to provide colony induction signaling peptide AIP and its applications.
The present invention protects polypeptide I first, as shown in the sequence 8 of sequence table.
The present invention also protects polypeptide II, amino acid shown in the sequence 8 containing ordered list.The polypeptide II is specifically such as sequence
Shown in the sequence 7 of table.Polypeptide II shown in the sequence 7 of sequence table is the precursor peptide of polypeptide I shown in the sequence 8 of sequence table.
The present invention also protects the application of the polypeptide I or the polypeptide II in preparing food.The food is concretely
Yoghourt.
The present invention also protects lactobacillus ljj-6 and application of the specific polypeptides in preparing food.The food is concretely
Yoghourt；The specific polypeptides are the polypeptide I or the polypeptide II.
The present invention also protects a kind of preparation method of sour milk product, includes the following steps：Lactobacillus ljj-6 is seeded to liquid
State milk, then ferments, and specific polypeptides are then added, and obtains sour milk product；The specific polypeptides are the polypeptide I or institute
State polypeptide II.
In the method, initial densities of the lactobacillus ljj-6 in the liquid milk is 104cfu/ml。
In the method, initial concentration of the specific polypeptides in fermentation system is 0.016 μM or more, preferably 0.016 μ
M-2μM。
The condition of the fermentation is concretely：37 DEG C of stationary cultures 12 hours.
The liquid milk is milk and/or goat milk.
The sour milk product that any description above method is prepared also belongs to protection scope of the present invention.
The present invention also protects the polypeptide I or the polypeptide II in promoting lactobacillus ljj-6 that quorum sensing phenomenon occurs
Application.The quorum sensing phenomenon is presented as the population density of regulation and control lactobacillus ljj-6.
The present invention also protects the application of the polypeptide I or the polypeptide II as bacteria quorum sensing signaling molecule.It is described to answer
In, the bacteria quorum sensing signaling molecule is the bacteria quorum sensing signaling molecule of lactobacillus ljj-6.
Lactobacillus delbruockii subspecies bulgaricus (Lactobacillusdelbrueckiisubsp.bulgaricus) ljj-
6, China Committee for Culture Collection of Microorganisms's common micro-organisms center has been preserved on 09 09th, 2015 (referred to as
CGMCC, address are：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica), preservation registration number
For CGMCC NO.11346.Lactobacillus delbruockii subspecies bulgaricus (Lactobacillus delbrueckiisubsp.bulgar
Icus) ljj-6, abbreviation lactobacillus ljj-6.
On the one hand, the culture that same time is carried out in MRS fluid nutrient mediums, at 12 hours, the nectar of lactobacillus ljj-6
Degree is similar with existing lactobacillus bulgaricus, and the strain density of lactobacillus ljj-6 is substantially less than existing Bao Jiali after 24 hours
Sub- lactobacillus.On the other hand, the culture of same time is carried out, acidity value and the addition of the Yoghourt that lactobacillus ljj-6 is obtained is added
The acidity value for the Yoghourt that existing lactobacillus bulgaricus obtains is not significantly different, but by after Yoghourt progress shelf life preservation, is added
The acidity value for entering the Yoghourt that lactobacillus ljj-6 is obtained is substantially less than the acidity that the Yoghourt that existing lactobacillus bulgaricus obtains is added
Value.The result shows that lactobacillus ljj-6 can solve post-acidification of yoghurt phenomenon, for the bulgarian milk with group's perceptual phenomenon
Bacillus strain.
The present invention has theory value for the research of quorum sensing phenomenon.The present invention can be used for improving in Yoghourt industry
Acidification phenomenon afterwards is worth Yoghourt industry with major application.
Description of the drawings
Fig. 1 is the acidity value result in embodiment 1.
Fig. 2 is the sequence alignment result in embodiment 2.
Fig. 3 is the Phylogenetic tree in embodiment 2.
Fig. 4 is the electrophoretogram using degenerate primer to progress PCR amplification in embodiment 2.
Fig. 5 is the electrophoretogram using special primer to progress PCR amplification in embodiment 2.
Fig. 6 is the bacterium solution OD in embodiment 3600nmIt is worth result.
Fig. 7 is the bacterium solution OD in embodiment 4600nmIt is worth result.
Specific implementation mode
Embodiment below facilitates a better understanding of the present invention, but does not limit the present invention.Experiment in following embodiments
Method is unless otherwise specified conventional method.Test material as used in the following examples is unless otherwise specified certainly
What routine biochemistry reagent shop was commercially available.Quantitative test in following embodiment is respectively provided with three repeated experiments, as a result makes even
Mean value.
The preparation method of MRS fluid nutrient mediums is as follows：10.0 grams of casein peptone, 10.0 grams of object of beef leaching, yeast is taken to carry
Take 5.0 grams of liquid, 5.0 grams of glucose, 5.0 grams of sodium acetate, 2.0 grams of lemon acid diamine, 1.0 grams of Tween 80, dipotassium hydrogen phosphate 2.0
Gram, 0.2 gram of epsom salt, 0.05 gram of seven water manganese sulfate and 20.0 grams of calcium carbonate, with distillation water dissolution and be settled to 1.0 liters,
Adjust pH to 6.8.
Lactobacillus bulgaricus LJJ：Cui Wenming etc., " analysis of Influential Factors of lactobacillus bulgaricus LJJ self-dissolvings ", food
With fermentation industry, the 7th phase (total 307th phase) of volume 39 in 2013,6-12.
Bacterial genomes DNA extraction kit (TIANGEN Bacteria DNA kit)：Tiangeng biochemical technology (Beijing)
Co., Ltd, catalog number DP302-02.
Embodiment 1, the identification of lactobacillus ljj-6, preservation and application
One, the identification and preservation of lactobacillus ljj-6
08 month 2012, isolated one plant of bacterium, was named as ljj-6 from the traditional zymotic dairy product of Inner Mongol pastoral area.
The morphological feature of ljj-6：Gram-positive bacillus, thalline is thick and grows, thalline grow 2 μm~9 μm, it is 0.15 μm wide~
0.18 μm, single body is in elongated rod shape or chaining, and thalline both ends are slightly round, usually in single, parallel or short chain arrangement.
The physiological and biochemical property of ljj-6：Facultative anaerobic bacteria, most suitable cultivation temperature are 3 DEG C, and the pH of optimum growth is 6.5-
7.0, optimum culture medium is MRS culture mediums；Lactose that can be in fermented milk generates lactic acid, while generating special perfume (or spice)
Gas.
The coded sequence (see the sequence 9 of sequence table) of the 16sRNA of ljj-6 and the Lactobacillus delbrueckii Bao Jiali in GenBank
The coding sequence homology of the 16sRNA of subspecies ATCC11842 is 98%.
Lactobacillus delbruockii subspecies bulgaricus (Lactobacillus delbrueckii provided by the invention
Subsp.bulgaricus it is general to be preserved in China Committee for Culture Collection of Microorganisms on 09 09th, 2015 by) ljj-6
(abbreviation CGMCC, address are at logical microorganism center：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Chinese Academy of Sciences microorganism are ground
Study carefully institute), preservation registration number is CGMCC NO.11346.Lactobacillus delbruockii subspecies bulgaricus (Lactobacillus
Delbrueckiisubsp.bulgaricus) ljj-6, abbreviation lactobacillus ljj-6.
Two, the culture (MRS culture mediums) of lactobacillus ljj-6
1, packet transaction is following (carrying out repeating to test three times, repeat 5 reprocessings of every group of setting in testing every time)：
First group：Lactobacillus ljj-6 is seeded to MRS fluid nutrient mediums and its initial concentration is made to be 104Cfu/ml, 37 DEG C
Stationary culture 20 hours；
Second group：Lactobacillus bulgaricus LJJ is seeded to MRS fluid nutrient mediums and its initial concentration is made to be 104cfu/
Ml, 37 DEG C of stationary cultures 20 hours.
2, in step 1, when stationary culture 12 hours, the OD of detection detection cultivating system600nmValue.First group of OD600nmValue
It is 2.4 ± 0.1, second group of OD600nmValue is 2.4 ± 0.15.
3, after completing step 1, the OD of cultivating system is detected600nmValue.
First group of OD600nmValue is 2.8 ± 0.1, second group of OD600nmValue is 3.45 ± 0.15.
4, after completing step 1, the pH value of cultivating system is detected.
First group of pH value 4.4 ± 0.2, second group of pH value 4.3 ± 0.2, there was no significant difference between two groups.
The result shows that：In incubation, the initial growth speed of lactobacillus ljj-6 and lactobacillus bulgaricus LJJ do not have
There were significant differences；With the increase of incubation time, the bacteria concentration in cultivating system is higher and higher, and lactobacillus ljj-6 is due to having
Quorum sensing phenomenon is slack-off to the speed of growth, and lactobacillus bulgaricus LJJ is not due to having group's perceptual phenomenon to raw
Long speed is not slack-off.
Three, the culture (milk) of lactobacillus ljj-6
1, packet transaction is following (carrying out repeating to test three times, repeat 5 reprocessings of every group of setting in testing every time)：
First group：Lactobacillus ljj-6 is seeded to ternary plain chocolate and its initial concentration is made to be 104Cfu/ml, 37 DEG C quiet
Set culture 12 hours；
Second group：Lactobacillus bulgaricus LJJ is seeded to ternary plain chocolate and its initial concentration is made to be 104Cfu/ml,
37 DEG C of stationary cultures 12 hours.
It is known as Yoghourt after being vaccinated with the plain chocolate stationary culture of lactobacillus.
2, after completing step 1, the acidity value of Yoghourt is detected.
The acidity of first group, second group Yoghourt is 60 ° of T-80 ° of T.
3, after completing step 1, Yoghourt is subjected to shelf life preservation (shelf life preserves i.e. 4-6 DEG C of preservation), sampling daily is simultaneously
Detect acidity.
(timing since preserving shelf life, every 24 hours are 1 day to the result is shown in Figure 1, and abscissa is to preserve number of days；Ordinate
For acidity value, unit is ° T).With the extension of holding time, the difference between first group and second group starts to show, lactobacillus
The acidity value rising of the Yoghourt of ljj-6 fermentations is slower, when preservation to the 10th day, the acid of the Yoghourt of lactobacillus ljj-6 fermentations
Yoghourt of the angle value significantly lower than lactobacillus bulgaricus LJJ fermentations.
Embodiment 2, the clonal population inductive signal peptide gene from lactobacillus ljj-6
The gram-positive bacteria to be measured used in the present embodiment is lactobacillus ljj-6.
One, sequence is collected from the encoding gene of published gram-positive bacteria colony induction signaling peptide
By retrieve GenBank databases andThe Gram-positive bacteria quorum sensing included in database
The coded sequence of signal peptide, in conjunction with the code sequence for the gram-positive bacteria colony induction signaling peptide being published at present on periodicals and magazines
Row are collected into the DNA sequence dna of 15 encoding Gram-positive bacteria quorum sensing signal peptides altogether (containing complete sequence and segment).
Two, flora phylogenetic analysis
1, the bacterial strain information for 15 sequences that obtaining step one obtains respectively, is then obtained from GenBank databases respectively
The 16s rDNA sequences of bacterial strain are taken, 15 16s rDNA sequences are obtained.
2,15 16s rDNA sequences for obtaining the 16s rDNA sequences of gram-positive bacteria to be measured and step 1 carry out sequence
Row compare, and comparison result is shown in Fig. 2.
3, the comparison result based on step 2 builds Phylogenetic tree, sees Fig. 3.Gram-positive bacteria to be measured is in genetic distance
On, distance Lactobacillus casei W56, Pediococcusclausseni i, Lactobacillus
This four plants of gram-positive bacteria affiliations of paraplantarum and Lactobacillus plantarum ZJ316 are nearest,
It is 95% or more.
Three, the design of degenerate primer
The four plant gram-positive bacterias nearest with gram-positive bacteria affiliation to be measured screened using step 2 is bases
Plinth carries out sequence alignment to the coded sequence of its colony induction signaling peptide, searches its conservative region, be designed according to conserved sequence
To following degenerate primer to (annealing temperature is 47 DEG C)：
Sense primer (sequence 1 of sequence table)：5’-CASSATTGAACACCTYCT-3’；
Downstream primer (sequence 2 of sequence table)：5’-CGYCTCTGAWTGCYYTGA-3’；
S=G or C, W=A or T, Y=C or T.
Four, the clone of the Partial Fragment of the encoding gene of the colony induction signaling peptide of gram-positive bacteria to be measured
The genomic DNA for extracting gram-positive bacteria to be measured, using the degenerate primer of step 3 design to carrying out PCR amplification
(electrophoretogram of pcr amplification product is shown in Fig. 4) recycles pcr amplification product and is sequenced.Pcr amplification product is 167bp, such as sequence table
Sequence 3 shown in.Sequence 3 is the Partial Fragment of the encoding gene for the colony induction signaling peptide for encoding gram-positive bacteria to be measured.
BLAST is carried out in GenBank databases, finds the Lactobacillus in sequence 3 and NCBI
The sequence of the positions 1286023-1286189 of 11842 strain gene groups of delbrueckiisubsp.bulgaricusATCC is kissed
It closes.
Five, the clone of the colony induction signaling peptide full length sequence of gram-positive bacteria to be measured
1, on the basis of the Partial Fragment of the encoding gene of the colony induction signaling peptide of gram-positive bacteria to be measured, reference
The genome of upper 11842 bacterial strains of Lactobacillus delbruecki i subsp.bulgaricus ATCC of Genbank
Full-length gene order in DNA where 1286023-1286189 nucleotide devises group's sense of clone's coding strain to be tested
The following special primer of the DNA molecular of induction signal peptide full length sequence is to (annealing temperature is 55.6 DEG C)：
Sense primer (sequence 4 of sequence table)：5’-ACGCGTCGACATGGCTAAAGTAG-3’；
Downstream primer (sequence 5 of sequence table)：5’-ACATGCATGCGTCGTCGCTGAT-3’.
2, the genomic DNA for extracting gram-positive bacteria to be measured, the special primer designed using step 1 is to carrying out PCR expansions
Increase (electrophoretogram of pcr amplification product is shown in Fig. 5), recycle pcr amplification product and is sequenced.Pcr amplification product is 1008bp, such as sequence
(being open reading frame from the nucleotide of 5 ' end 24-986) shown in the sequence 6 of table, it is more shown in the sequence 7 of polynucleotide
Peptide (is made of) 320 amino acid residues, i.e. the colony induction signaling peptide full length sequence of strain to be tested.
Six, the colony induction signaling peptide maturation peptide sequence analysis of strain to be tested
According to current existing document report, in gram-positive bacteria intervention school-based, there is biological activity, hair
Wave signaling molecule effect is typically the small peptide of 15-30 amino acid residue composition, and the precursor peptide of gram-positive bacteria transcription must
Effect of the palpus Jing Guo internal digestion, generates the mature peptide of short-movie section, can just dissociate and play the effect of signal transduction to outside thalline.
Colony induction signaling peptide full length sequence is analyzed using SignalP 4.1, obtains 26 amino acid residues composition
Ripe peptide sequence (see the sequence 8 of sequence table).
Polypeptide shown in sequence 8 by sequence table is named as colony induction signaling peptide AIP, abbreviation AIP.
The functional verification and application of embodiment 3, AIP
1, polypeptide (AIP) shown in the sequence 8 of artificial synthesized sequence table.
2, packet transaction is following (carrying out repeating to test three times, repeat 5 reprocessings of every group of setting in testing every time)：
First group：Lactobacillus ljj-6 is seeded to the MRS fluid nutrient mediums containing 2 μM of AIP and makes its initial concentration be
104Cfu/ml, 37 DEG C of stationary cultures 32 hours；
Second group：Lactobacillus ljj-6 is seeded to MRS fluid nutrient mediums and its initial concentration is made to be 104Cfu/ml, 37 DEG C
Stationary culture 32 hours.
In incubation, every 2 hours sampling monitoring bacterium solutions OD600nmValue, records its changing rule.
As a result see Fig. 6.As a result it shows：It is added in the cultivating system of 2 μM of AIP, the lactobacillus ljj-6 speeds of growth are slower；
When culture is to 16h, it is not added with the presented apparent cloudy state of cultivating system of AIP, but identical incubation time contains 2
Macroscopic muddiness is not presented for the cultivating system of μM AIP；It adds AIP and is not added with lactobacillus ljj- in the cultivating system of AIP
There is 6 growth curve apparent difference (to be not added in the cultivating system of AIP, OD600nmValue first increases, and reaches highest when to 20h
Point then slowly reduces；In the cultivating system for adding AIP, OD600nmValue although overall also have raised trend, curve
The gradient is significantly lower than the cultivating system for being not added with AIP, and curve is integrally than shallower.The result shows that addition AIP can regulate and control newborn bar
The population density of bacterium ljj-6, polypeptide shown in sequence 8 are colony induction signaling peptide really.
Embodiment 4, AIP inhibit the concentration threshold of lactobacillus ljj-6 population densities
1, polypeptide (AIP) shown in the sequence 8 of artificial synthesized sequence table.
2, packet transaction is following (carrying out repeating to test three times, repeat 5 reprocessings of every group of setting in testing every time)：
First group：Lactobacillus ljj-6 is seeded to the MRS fluid nutrient mediums containing 2 μM of AIP and makes its initial concentration be
104Cfu/ml, 37 DEG C of stationary cultures 32 hours；
Second group：Lactobacillus ljj-6 is seeded to the MRS fluid nutrient mediums containing 0.4 μM of AIP and makes its initial concentration be
104Cfu/ml, 37 DEG C of stationary cultures 32 hours；
Third group：Lactobacillus ljj-6 is seeded to the MRS fluid nutrient mediums containing 0.08 μM of AIP and makes its initial concentration be
104Cfu/ml, 37 DEG C of stationary cultures 32 hours；
4th group：Lactobacillus ljj-6 is seeded to the MRS fluid nutrient mediums containing 0.016 μM of AIP and makes its initial concentration
It is 104Cfu/ml, 37 DEG C of stationary cultures 32 hours；
5th group：Lactobacillus ljj-6 is seeded to the MRS fluid nutrient mediums containing 0.0032 μM of AIP and makes its initial concentration
It is 104Cfu/ml, 37 DEG C of stationary cultures 32 hours；
6th group：Lactobacillus ljj-6 is seeded to MRS fluid nutrient mediums and its initial concentration is made to be 104Cfu/ml, 37 DEG C
Stationary culture 32 hours.
In incubation, every 2 hours sampling monitoring bacterium solutions OD600nmValue, records its changing rule.
As a result see Fig. 7.Under non-AIP environment or 0.0032 μM of concentration, growth curve has obvious lactobacillus ljj-6
First increase the trend reduced afterwards, the peak of growth is reached in 20h, it is contemplated that had in experimentation thalline itself secretion
Signal peptide be diffused into ambient enviroment, it is possible to assert that 0.0032 μM of AIP initially adds concentration and is not up to lactobacillus
The threshold concentration of environmental signal peptide when ljj-6 generation quorum sensings.Under 0.016 μM of AIP concentration, growth curve although
Have a slow downward trend after first increasing, but the growth curve and the growth curve under 2 μM of AIP concentration have no it is obviously poor
It is different, the threshold concentration of signal peptide in environment when quorum sensing occurs thus may determine that 0.016 μM of AIP is lactobacillus ljj-6.
Embodiment 5 prepares Yoghourt using lactobacillus ljj-6 and AIP
1, polypeptide (AIP) shown in the sequence 8 of artificial synthesized sequence table.
2, packet transaction is following (carrying out repeating to test three times, repeat 5 reprocessings of every group of setting in testing every time)：
First group：Lactobacillus ljj-6 is seeded to ternary plain chocolate and its initial concentration is made to be 104Cfu/ml, 37 DEG C quiet
Set culture 12 hours；
Second group：Lactobacillus ljj-6 is seeded to ternary plain chocolate and its initial concentration is made to be 104Cfu/ml, 37 DEG C quiet
AIP is added in system after setting culture 12 hours and its initial concentration is made to be 2 μM.
It is known as Yoghourt after being vaccinated with the plain chocolate stationary culture of lactobacillus.
2, after completing step 1, the acidity value of Yoghourt is detected.
The acidity of first group, second group Yoghourt is 60 ° of T-80 ° of T.
3, after completing step 1, Yoghourt is subjected to shelf life preservation (shelf life preserves i.e. 4-6 DEG C of preservation), sampling daily is simultaneously
Detect acidity.
When preservation to the 10th day, the acidity value of first group of Yoghourt is 95 ° of T, and the acidity value of second group of Yoghourt is 80 ° of T.
Claims (10)
1. polypeptide I, amino acid sequence is as shown in SEQ ID No.8.
2. application of the polypeptide I described in claim 1 in preparing food.
3. the application of specific polypeptides and specific strain in preparing food；The specific polypeptides are polypeptide I described in claim 1；
The specific strain is lactobacillus delbruockii subspecies bulgaricus（Lactobacillusdelbrueckiisubsp.bulgaricu s）Ljj-6, deposit number are CGMCC NO.11346.
4. a kind of preparation method of sour milk product, includes the following steps：Specific strain is seeded to liquid milk, is then sent out
Then specific polypeptides are added in ferment, obtain sour milk product；The specific strain is lactobacillus delbruockii subspecies bulgaricus
（Lactobacillusdelbrueckiisubsp.bulgaricus）Ljj-6, deposit number are CGMCC NO.11346；Institute
It is polypeptide I described in claim 1 to state specific polypeptides.
5. method as claimed in claim 4, it is characterised in that：In the method, the specific strain is in the liquid milk
Initial density be 104cfu/ml。
6. method as described in claim 4 or 5, it is characterised in that：In the method, the specific polypeptides are in fermentation system
Initial concentration be 0.016 μM or more.
7. method as described in claim 4 or 5, it is characterised in that：In the method, the specific polypeptides are in fermentation system
Initial concentration be 0.016 μM -2 μM.
8. the sour milk product that any the method is prepared in claim 4 to 7.
9. application of the polypeptide I described in claim 1 in promoting specific strain that quorum sensing phenomenon occurs；The specific strain
For lactobacillus delbruockii subspecies bulgaricus（Lactobacillusdelbrueckiisubsp.bulgaricus）Ljj-6 is protected
It is CGMCC NO.11346 to hide number.
10. application of the polypeptide I described in claim 1 as bacteria quorum sensing signaling molecule；The bacterium is specially Lactobacillus delbrueckii
Subspecies bulgaricus（Lactobacillusdelbrueckiisubsp.bulgaricus）Ljj-6, deposit number CGMCC
NO.11346。
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