CN105267046B - A kind of quick method for repairing demineralization dentine - Google Patents
A kind of quick method for repairing demineralization dentine Download PDFInfo
- Publication number
- CN105267046B CN105267046B CN201510730463.8A CN201510730463A CN105267046B CN 105267046 B CN105267046 B CN 105267046B CN 201510730463 A CN201510730463 A CN 201510730463A CN 105267046 B CN105267046 B CN 105267046B
- Authority
- CN
- China
- Prior art keywords
- dentine
- demineralization
- calcium phosphate
- amorphous calcium
- remineralization
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Dental Preparations (AREA)
Abstract
The present invention relates to a kind of quick method for repairing demineralization dentine.The existing method for repairing demineralization dentine is use two kinds of dentin non-collagenous protein analogs, is realized by means of Portland cement slow-releasing system, but this square law device is excessively complicated, reaches the time need 4 months as long as needed for holostrome mineralising.The inventive method first by the use of the polyacrylic acid that simplicity is easy to get as conditioning agent, has the amorphous calcium phosphate particle of mobility using calcium ion and phosphonium ion as raw material, 15 20nm sizes of synthesis；Then the dentine by the use of glutamic acid as the remineralization demineralization within 2 days of mineralising accelerator, has recovered the natural hierarchical organization of demineralization dentine, and the mechanical property of the dentine after remineralization also reached with natural dentine similar in it is horizontal.Of the invention on the basis of same restoration grade structure and mechanical property is reached, device is simple compared with existing Remineralizing technique, takes short, more efficiently remineralization demineralization dentine this problem of solving.
Description
Technical field
The invention belongs to technical field of biological materials, is related to a kind of quick method for repairing demineralization dentine.
Background technology
In dentine bonding process, the exposed collagen of mixed layer substrate can not be fully wrapped around by binding agent, and exposure collagen meets with
The degraded of endogenous matrix metalloproteinase is to shorten the key factor of resin-dentine bonding complex durability.Moderate and depth
Degree carious tooth lesion is also mainly shown as the demineralization of dentin layer.Solve these problems and be directed to carry out again the dentine of demineralization
Mineralising.With going deep into for research, dentine microstructure, into contacting between ore deposit site and collagenous fibres, non-collagen exists
Effect and biomineralization process in dentine biomineralization are gradually disclosed by scientist, and human simulation in vitro, demineralization
The reparation field of dentine enters the epoch of bionical remineralization.The bionical remineralization mode of main flow is at present：With two kinds of tooth sheets
The bionical analog of matter non-collagen, success in the solution containing phosphate in source is being sustained using Portland Cement as calcium ion
Ground makes the dentine remineralization of acid etching demineralization, and generates mineral and the outer mineral of fiber in fiber.But such method takes
For four months, do not possess Clinical practicability.Moreover, the device is by calcium ion sustained-release source, phosphonium ion source, non-collagen class
Formed like thing etc., too complex, it is difficult to allow clinician to be freed from busy work.
In recent years for research of the nano-scale amorphous calcium phosphate granules as the presoma during being present in biomineralization
It is more and more deep, there is scholar's research to show to be up to 10-20% water content and interior atoms inside the particle between crystal and solution
Disorderly arranged " mobility " for imparting its similar liquids, amorphous granular by " mobility " in advance replicate template pattern
And structure, then gradual dehydration, crystallization, solidification generate the more stable crystalline state mineral of thermodynamics.Amorphous calcium phosphate it is basic
Component units are Ca9(PO4)6(Posner Cluster), this material are referred to as " small cluster ", and size is about 0.95 nm,
Can mutually it reunite for larger-sized mineral grain.
The content of the invention
It is an object of the invention to overcome the deficiencies of the prior art and provide a kind of quick method for repairing demineralization dentine.
The present invention central scope be regulate and control glutamic acid concentration so as to control Posner Cluster transformation time,
Connect the speed that have adjusted dentine biology remineralization, can greatly shorten in the method demineralization dentine remineralization needs when
Between.
The technical solution taken by the invention to solve the above technical problem is：
The present invention synthesizes amorphous calcium phosphate particle first, accelerates the remineralization of demineralization dentine using mineralising accelerator.
Described synthesis amorphous calcium phosphate particle is specifically：
By the use of 8~10 g/L polyacrylic acid as the stabilizer of amorphous calcium phosphate, 400 mM glutamic acid solutions are as ore deposit again
Change accelerator.At 36~38 DEG C, the disodium hydrogen phosphate aqueous solution and 0.2~0.3 that 0.1~0.2 M, PH is 9.5 ± 0.1 is used
M calcium chloride waters are made into mother liquor；Using 50 ml syringes in 30~40 minutes by calcium chloride water by volume 1：1
Add into the disodium hydrogen phosphate aqueous solution, magnetic stirrer is used in course of reaction, and it is molten using 0.1 M sodium hydroxides
Liquid regulation and control pH is maintained at 9.5 ± 0.5 sections；So that calcium ion ultimate density is 9.0~11.0 mM, phosphonium ion it is final
Concentration is 5.0~7.0 mM, and the ultimate density of glutamic acid is 10~100 Mm.The ultimate density of polyacrylic acid is 300~1000
µg/mL；A diameter of 15~20 nm amorphous calcium phosphate particle is finally synthesized, is stored in the form of a solution after particle synthesis；
Described remineralization demineralization dentine is specifically：
Step 1. collects the Isolated Tooth of no dental caries, abrades enamel using turbine and is checked under Stereo microscope；Slowly
Fast ISOMET diamonds slicer takes the dentine piece that thickness is 0.45~1mm perpendicular to long axis of tooth；
Above-mentioned tooth piece is put into the phosphoric acid that mass concentration is 30~35% by step 2., and phosphoric acid is stirred with magnetic force in acid corrosion process
Sub- stirring is mixed, is taken out after 10 seconds, is rinsed with substantial amounts of redistilled water, it is de- in 2~5 micrometer ranges to obtain de- seam thickness
Ore deposit dentin layer；
Step 3. takes the amorphous calcium phosphate particle solution of the above-mentioned synthesis of 20~50 ml to be placed in sample bottle, by demineralization tooth
Essence is placed in containing in amorphous calcium phosphate particle solution, and blake bottle is sealed；
Above-mentioned sample bottle is placed in 35~38 DEG C of insulating boxs by step 4., is taken out after 2 days.
Beneficial effects of the present invention：With good biocompatibility；Meet proposed in demineralization dentine reparation " most
Limits preserve tissue of tooth " principle, remineralization can be carried out by template of existing demineralization fiber；Compared to traditional mineralising method, sheet
Method can recover the mineral content of demineralization dentine, Protection glue in a short time by the supreme carry out mineral growth in bottom
Original is not corroded by matrix metalloproteinase, prevents demineralization from further occurring, and is advantageous to maintain good resin-dentine bonding
Complex durability；The special hierarchical organization of dentine is recovered；Recover the mechanical property of dentine；It is bionical compared to existing
Mineralising method, raw material are easy to get, and system is simple；Importantly, the time required for mineralising is completed in this method greatly shortens, it is only
2 days, be a kind of breakthrough for remineralization demineralization dentine technology.
Brief description of the drawings
Fig. 1 a are the ESEM of demineralization dentin collagen in embodiment 1,2,3,4（SEM）Picture.
Fig. 1 b are the transmission electron microscope of demineralization dentin collagen in embodiment 1,2,3,4（TEM）Picture.
Fig. 2 a are that the different amorphous calcium phosphate reparations under the polyacrylic acid regulation and control that concentration is 50 ug/mL in embodiment 1 take off
The ESEM of ore deposit dentine（SEM）Picture；Fig. 2 b are under the polyacrylic acid regulation and control that concentration is 200 ug/mL in embodiment 2
Different amorphous calcium phosphates repair the ESEM of demineralization dentine（SEM）；Fig. 2 c are that concentration is 50 ug/mL in embodiment 1
Different amorphous calcium phosphates under polyacrylic acid regulation and control repair the transmission electron microscope of demineralization dentine（TEM）；Fig. 2 d are in embodiment 1
Different amorphous calcium phosphates under the polyacrylic acid regulation and control that concentration is 200 ug/mL repair the transmission electron microscope of demineralization dentine
（TEM）.
The amorphous calcium phosphate particle for the polyacrylic acid regulation and control synthesis that Fig. 3 a are 350 ug/mL in embodiment 3 was at two days
The displaing micro picture of collagenous fibres in the demineralization dentine of reparation；The polyacrylic acid regulation and control that Fig. 3 b are 350 ug/mL in embodiment 3 are closed
Into the demineralization dentine repaired at four days of amorphous calcium phosphate particle in collagenous fibres displaing micro picture；Fig. 3 c are embodiment
The demineralization dentine that the amorphous calcium phosphate particle of 350 ug/mL polyacrylic acid regulation and control synthesis was repaired at four days in 3 is indulged
Section picture；Fig. 3 d are the amorphous phosphorus that the polyacrylic acid of 350 ug/mL in embodiment 3 synthesizes with 10 mM Glu coordinated regulations
The displaing micro picture of collagenous fibres in the demineralization dentine that sour calcium particle was repaired at two days；Fig. 3 e are 350 ug/mL in embodiment 3
The demineralization dentine repaired at four days of polyacrylic acid and the amorphous calcium phosphate particle that 10 mM Glu coordinated regulations synthesize in
The displaing micro picture of collagenous fibres；The polyacrylic acid that Fig. 3 f are 350 ug/mL in embodiment 3 synthesizes with 10 mM Glu coordinated regulations
The longitudinal section picture of demineralization dentine repaired at four days of amorphous calcium phosphate particle.
The amorphous calcium phosphate particle for the polyacrylic acid regulation and control synthesis that Fig. 4 a are 350 ug/mL in embodiment 3 is when different
Between section it is infrared（FTIR）Picture；Fig. 4 b are that 350 ug/mL polyacrylic acid and 10 mM Glu coordinated regulations close in embodiment 4
Into amorphous calcium phosphate particle in the infrared of different time sections（FTIR）Picture；Fig. 4 c are the poly- of 350 ug/mL in embodiment 3
Transmission electron microscope of the amorphous calcium phosphate particle of acrylic acid regulation and control synthesis at 2 days（TEM）Picture；Fig. 4 d are 350 in embodiment 4
The transmission electron microscope of ug/mL polyacrylic acid and the amorphous calcium phosphate particle that 10 mM Glu coordinated regulations synthesize at 2 days
（TEM）Picture；Fig. 4 e are to cleave peak value in embodiment 3,4（Splitting function, SF）Calculate schematic diagram；In Fig. 4 f
The amorphous calcium phosphate particle for the polyacrylic acid regulation and control synthesis that broken line of keeping right is 350 ug/mL in embodiment 3 is in different time
SF collection of illustrative plates, the broken line that keeps left in Fig. 4 f synthesize for the polyacrylic acid of 350 ug/mL in embodiment 4 with 10 mM Glu coordinated regulations
Amorphous calcium phosphate particle different time SF collection of illustrative plates.
Different amorphous calcium phosphate particles under the polyacrylic acid regulation and control that lower curve is 50 ug/mL in embodiment 1 in Fig. 5 a
It is infrared at two days（FTIR）Collection of illustrative plates, under the polyacrylic acid regulation and control that upper curve is 200 ug/mL in embodiment 2 in Fig. 5 a not
It is infrared at two days with amorphous calcium phosphate particle（FTIR）Collection of illustrative plates；Fig. 5 b are the polyacrylic acid of 50 ug/mL in embodiment 1
Transmission electron microscope of the amorphous calcium phosphate particle at two days under regulation and control（TEM）Picture；Fig. 5 c are 200 ug/mL in embodiment 2
Polyacrylic acid regulation and control under transmission electron microscope of the amorphous calcium phosphate particle at two days（TEM）Picture.
The amorphous calcium phosphate particle for the polyacrylic acid regulation and control synthesis that Fig. 6 is 350 ug/mL in embodiment 3 is when different
350 ug/mL polyacrylic acid and 10 mM Glu are assisted in the nano impress intensity and embodiment 4 of the demineralization dentine of interior reparation
The nano impress intensity for the demineralization dentine repaired with the amorphous calcium phosphate particle of regulation and control synthesis in different time.
Embodiment
The present invention is expanded on further with reference to the accompanying drawings and examples：
Embodiment 1：
It is equipped with 0.25M calcium chloride（CaCl2）The aqueous solution, 0.15M disodium hydrogen phosphates（Na2HPO4）The aqueous solution, 10g/
L polyacrylic acid（PAA）The aqueous solution does the mother liquor of compound experiment.Take 2.4 mL Na2HPO4 mother liquors and 3.30mL PAA female
Liquid mixes, and redistilled water dilution, adjusts pH to 9.5 ± 0.1, the h of stirring at normal temperature 0.5 with the 1M NaOH aqueous solution, always
Volume is 30 mL.2.4 mL CaCl2 mother liquors are taken to be diluted with water to 30 mL.30 mL calcium liquid 30 are added with buret
Enter 30 mL phosphorus solutions, magnetic stirrer during dropwise addition, 0.1M NaOH solution regulation and control pH is maintained at 9.5 ±
0.5 section.Finally, calcium ion concentration is 10.0 mM, and phosphate ion concentration is 6.0 mM, and PAA concentration is 50 ug/mL.Ensure
Whole dropwise addition process continues 25~30 minutes.The Isolated Tooth without dental caries is collected, is grown with ISOMET diamonds slicer at a slow speed perpendicular to tooth
Axle first cuts off enamel, then cuts the dentine piece that thickness is 0.5~1 mm；Then with 500 # -2000 # sand paper one by one
Polishing, a large amount of redistilled water cleanings；Above-mentioned tooth piece is put into the phosphoric acid that mass concentration is 35%, takes out after 10 seconds, use again
Substantial amounts of redistilled water rinses, and obtains de- demineralization dentine model of the seam thickness in 2~3 micrometer ranges；Take on 15 ml
The amorphous calcium phosphate particle solution for stating synthesis is placed in blake bottle, and demineralization dentine model is placed in into amorphous calcium phosphate particle
In solution；Above-mentioned blake bottle is placed in 37.5 DEG C of insulating boxs, taken out after 2,4 days.By the dentine sample of remineralization
It is divided into two, half is dried with the mode of alcohol serial dehydration, does ESEM（SEM）With the sight of transmission electron microscope (TEM)
Examine, second half does not make dewater treatment, directly carries out nano impress detection.
Embodiment 2：
It is equipped with 0.25 M calcium chloride（CaCl2）The aqueous solution, 0.15M disodium hydrogen phosphates（Na2HPO4）The aqueous solution, 10 g/
L polyacrylic acid（PAA）The aqueous solution does the mother liquor of compound experiment.Take 2.4 mL Na2HPO4 mother liquors and 1.2 mL PAA female
Liquid mixes, and redistilled water dilution, adjusts pH to 9.5 ± 0.1, the h of stirring at normal temperature 0.5 with the 1M NaOH aqueous solution, always
Volume is 30 mL.Take 2.4 mL CaCl2Mother liquor is diluted with water to 30 mL.30 mL calcium liquid 30 are added with buret
Enter 30 mL phosphorus solutions, magnetic stirrer during dropwise addition, 0.1M NaOH solution regulation and control pH is maintained at 9.5 ±
0.5 section.Finally, calcium ion concentration is 10.0 mM, and phosphate ion concentration is 6.0 mM, and PAA concentration is 200 ug/mL.Ensure
Whole dropwise addition process continues 25~30 minutes.The Isolated Tooth without dental caries is collected, is grown with ISOMET diamonds slicer at a slow speed perpendicular to tooth
Axle first cuts off enamel, then cuts the dentine piece that thickness is 0.5~1mm；Then beaten one by one with 500 # -2000 # sand paper
Mill, a large amount of redistilled water cleanings；Above-mentioned tooth piece is put into the phosphoric acid that mass concentration is 35%, taken out after 10 seconds, again with big
The redistilled water of amount rinses, and obtains de- demineralization dentine model of the seam thickness in 2~3 micrometer ranges；Take 15 ml above-mentioned
The amorphous calcium phosphate particle solution of synthesis is placed in blake bottle, and it is molten that demineralization dentine model is placed in into amorphous calcium phosphate particle
In liquid；Above-mentioned blake bottle is placed in 37.5 DEG C of insulating boxs, taken out after 2,4 days.By the dentine sample one of remineralization
It is divided into two, half is dried with the mode of alcohol serial dehydration, does ESEM（SEM）With the observation of transmission electron microscope (TEM),
Second half does not make dewater treatment, directly carries out nano impress detection.
Embodiment 3：
It is equipped with 0.25M calcium chloride（CaCl2）The aqueous solution, 0.15M disodium hydrogen phosphates（Na2HPO4）The aqueous solution, 10g/L
Polyacrylic acid（PAA）The aqueous solution does the mother liquor of compound experiment.Take 2.4 mL Na2HPO4Mother liquor and 2.1 mL PAA mother liquors mix
Close, redistilled water dilution, pH to 9.5 ± 0.1, the h of stirring at normal temperature 0.5, cumulative volume are adjusted with the 1M NaOH aqueous solution
For 30 mL.2.4 mL CaCl2 mother liquors are taken to be diluted with water to 30 mL.30 mL calcium liquid 30 are added with buret
30 mL phosphorus solutions, magnetic stirrer during dropwise addition, 0.1M NaOH solution regulation and control pH are maintained at 9.5 ± 0.5
Section.Calcium ion, phosphonium ion ultimate density are respectively 10.0 mM and 6.0 mM.Finally, calcium ion concentration is 10.0 mM,
Phosphate ion concentration is 6.0 mM, and PAA concentration is 350 ug/mL.Ensure that whole dropwise addition process continues 25~30 minutes.Collect without dental caries
Isolated Tooth, first cut off enamel perpendicular to long axis of tooth with ISOMET diamonds slicer at a slow speed, then it is 0.5~1mm to cut thickness
Dentine piece；Then polished one by one with 500 # -2000 # sand paper, a large amount of redistilled water cleanings；Above-mentioned tooth piece is put
Enter in the phosphoric acid that mass concentration is 35%, taken out after 10 seconds, rinsed again with substantial amounts of redistilled water, obtain de- seam thickness
Demineralization dentine model in 2~3 micrometer ranges；The amorphous calcium phosphate particle solution of the above-mentioned synthesis of 15ml is taken to be placed in culture
In bottle, demineralization dentine model is placed in amorphous calcium phosphate particle solution；Above-mentioned blake bottle is placed on 37.5 DEG C of insulating boxs
In, taken out after 7 days.The dentine sample of remineralization is divided into two, half is dried with the mode of alcohol serial dehydration,
Do ESEM（SEM）With the observation of transmission electron microscope (TEM), second half does not make dewater treatment, directly carries out nano impress detection.
Embodiment 4：
It is equipped with 0.25M calcium chloride（CaCl2）The aqueous solution, 0.15M disodium hydrogen phosphates（Na2HPO4）The aqueous solution, 10g/L
Polyacrylic acid（PAA）The aqueous solution does the mother liquor of compound experiment.Take 2.4 mL Na2HPO4Mother liquor and 2.1 mL PAA mother liquors,
The mM Glu mother liquors of 0.75 ml 400 mix, redistilled water dilution, with the 1M NaOH aqueous solution adjust pH to 9.5 ±
0.1, the h of stirring at normal temperature 0.5, cumulative volume are 30 mL.Take 2.4 mL CaCl2Mother liquor is diluted with water to 30 mL.With titration
30 mL calcium liquid 30 are added 30 mL phosphorus solutions, magnetic stirrer during dropwise addition, 0.1M NaOH solution by pipe
Regulation and control pH is maintained at 9.5 ± 0.5 sections.Calcium ion, phosphonium ion ultimate density are respectively 10.0 mM and 6.0 mM.Most
Eventually, calcium ion concentration is 10.0 mM, and phosphate ion concentration is 6.0 mM, and PAA concentration is 350 ug/mL, and Glu concentration is 10 mM.
Ensure that whole dropwise addition process continues 25~30 minutes.Collect the Isolated Tooth without dental caries, with ISOMET diamonds slicer at a slow speed perpendicular to
Long axis of tooth first cuts off enamel, then cuts the dentine piece that thickness is 0.5~1mm；Then with 500 # -2000 # sand paper by
One polishing, a large amount of redistilled water cleanings；Above-mentioned tooth piece is put into the phosphoric acid that mass concentration is 35%, taken out after 10 seconds, again
Rinsed with substantial amounts of redistilled water, obtain de- demineralization dentine model of the seam thickness in 2~3 micrometer ranges；Take 15ml
The amorphous calcium phosphate particle solution of above-mentioned synthesis is placed in blake bottle, and demineralization dentine model is placed in into amorphous calcium phosphate
In grain solution；Above-mentioned blake bottle is placed in 37.5 DEG C of insulating boxs, taken out after 7 days.The dentine sample one of remineralization is divided
For two, half is dried with the mode of alcohol serial dehydration, does ESEM（SEM）With the observation of transmission electron microscope (TEM), separately
Half does not make dewater treatment, directly carries out nano impress detection.
Fig. 1 is the ESEM of demineralization dentin collagen in embodiment 1,2,3,4（SEM）And transmission electron microscope（TEM）Picture.
It can be seen that remaining collagenous fibres rack after demineralization, contrast is low in transmission electron microscope imaging.
Fig. 2 and Fig. 3 (a, b, c) is embodiment 1,2,3, the different amorphous phosphoric acid under middle various concentrations polyacrylic acid regulation and control
Calcium repairs the ESEM of demineralization dentine（SEM）Picture.It can be seen that dentin collagen stromal surface forms layer mineral, it is single
Collagenous fibres obtain mineralising, and collagenous fibres are still exposed.
Fig. 3（d,e,f）The amorphous calcium phosphate reparation of polyacrylic acid and 10 Mm Glu coordinated regulations for 350 ug/mL
The scanning electron microscope (SEM) photograph of demineralization dentine, it is seen that demineralization dentine holostrome has completed mineralising in fiber in two days.It can be seen that
Mineralized layer mineral density and mineral order are suitable with the natural dentine of substrate, and demineralization dentine has obtained good reparation.
Fig. 4 is that 350 ug/mL polyacrylic acid, 350 ug/mL polyacrylic acid and 10 Mm Glu are adjusted in embodiment 3,4
The amorphous calcium phosphate particle for controlling synthesis is infrared（FTIR）And splitting peak value spectrogram.Transmission electron microscope（TEM）Picture.350 ug/mL
Polyacrylic acid and 10 Mm Glu regulation and control synthesis amorphous calcium phosphate particle be changed into hydroxyapatite in second day phase, and
The amorphous calcium phosphate particle of 350 ug/mL polyacrylic acid control synthesis was mutually changed into hydroxyapatite at the 4th day.
The amorphous calcium phosphate particle for the polyacrylic acid regulation and control synthesis that Fig. 5 is 50,200 ug/mL in embodiment 1,2 is infrared
（FTIR）Collection of illustrative plates, and transmission electron microscope（TEM）Picture.It can be seen that amorphous calcium phosphate particle was mutually changed into hydroxyl phosphorus at 24 hours
Lime stone.
The amorphous calcium phosphate and 350 ug/mL for the polyacrylic acid regulation and control synthesis that Fig. 6 is 350 ug/mL in embodiment 3,4
Polyacrylic acid and 10 Mm Glu regulation and control synthesis amorphous calcium phosphate particle repair demineralization dentine nano impress picture.
The demineralization dentine mechanics of 350 ug/mL polyacrylic acid and the amorphous calcium phosphate particle mineralising of 10 Mm Glu regulation and control synthesis
Performance is significantly strengthened than demineralization dentine, close to natural dentine.
Claims (1)
1. a kind of quick method for repairing demineralization dentine, this method synthesize amorphous calcium phosphate particle first, rush is then utilized
Mineralizer remineralization demineralization dentine, it is characterised in that：
Described synthesis amorphous calcium phosphate particle is specifically：
Promote by the use of 8~10 g/L polyacrylic acid as the stabilizer of amorphous calcium phosphate, 400 mM glutamic acid solutions as remineralization
Enter agent；At 36~38 DEG C, the disodium hydrogen phosphate aqueous solution and 0.2~0.3 M that 0.1~0.2 M, pH is 9.5 ± 0.1 are used
Calcium chloride water is made into mother liquor；Using 50 ml syringes in 30~40 minutes by calcium chloride water by volume 1：1 adds
Enter into the disodium hydrogen phosphate aqueous solution, magnetic stirrer is used in course of reaction, and use 0.1 M sodium hydroxide solutions
Regulation and control pH is maintained at 9.5 ± 0.5 sections；So that calcium ion ultimate density is 9.0~11.0 mM, phosphonium ion it is final dense
Spend for 5.0~7.0 mM, the ultimate density of glutamic acid is 10~100 mM；The ultimate density of polyacrylic acid is 300~1000 μ
g/mL；A diameter of 15~20 nm amorphous calcium phosphate particle is finally synthesized, is stored in the form of a solution after particle synthesis；
Described remineralization demineralization dentine is specifically：
Step 1. collects the Isolated Tooth of no dental caries, abrades enamel using turbine and is checked under Stereo microscope；At a slow speed
ISOMET diamonds slicer takes the dentine piece that thickness is 0.45~1mm perpendicular to long axis of tooth；
Above-mentioned tooth piece is put into the phosphoric acid that mass concentration is 30~35% by step 2., phosphoric acid magnetic stir bar in acid corrosion process
Stirring, takes out after 10 seconds, is rinsed with substantial amounts of redistilled water, obtains de- demineralization tooth of the seam thickness in 2~5 micrometer ranges
Intrinsic layer；
Step 3. takes the amorphous calcium phosphate particle solution of the above-mentioned synthesis of 20~50 ml to be placed in sample bottle, by demineralization dentine
It is placed in containing in amorphous calcium phosphate particle solution, blake bottle is sealed；
Above-mentioned sample bottle is placed in 35~38 DEG C of insulating boxs by step 4., is taken out after 2 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510730463.8A CN105267046B (en) | 2015-11-02 | 2015-11-02 | A kind of quick method for repairing demineralization dentine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510730463.8A CN105267046B (en) | 2015-11-02 | 2015-11-02 | A kind of quick method for repairing demineralization dentine |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105267046A CN105267046A (en) | 2016-01-27 |
| CN105267046B true CN105267046B (en) | 2018-03-30 |
Family
ID=55137455
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510730463.8A Active CN105267046B (en) | 2015-11-02 | 2015-11-02 | A kind of quick method for repairing demineralization dentine |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105267046B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4023204A1 (en) * | 2020-12-30 | 2022-07-06 | Stick Tech OY | A kit of parts for dental restoration |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106539693B (en) * | 2016-10-13 | 2019-10-29 | 浙江大学 | The method that macromolecule filming material loads the biomimetic mineralization pad pasting and its external evoked remineralization of ACP |
| CN108324578B (en) * | 2017-01-17 | 2020-02-14 | 武汉大学 | Liquid-phase mineralized precursor and method for repairing demineralized dentin |
| CN107028769A (en) * | 2017-04-20 | 2017-08-11 | 四川大学 | A kind of binding agent and its application for being used to simulate the pathologic oral environment of asialia |
| CN107184401A (en) * | 2017-06-02 | 2017-09-22 | 四川大学 | A kind of method of long-acting promotion demineralization dentine remineralization |
| CN109401157B (en) * | 2018-11-30 | 2021-02-09 | 中国科学院金属研究所 | Amorphous calcium phosphate-polyacrylic acid hybrid nano material and preparation method and application thereof |
| CN110693724B (en) * | 2019-11-05 | 2020-10-30 | 浙江大学 | Tooth mineralizing liquid and mineralizing method thereof |
| CN111714385A (en) * | 2020-06-03 | 2020-09-29 | 温州医科大学 | Formula and application of composite micro-nano particles |
| CN114939068B (en) * | 2022-06-28 | 2023-04-21 | 四川大学 | Amorphous calcium phosphate composite material, preparation method thereof and application thereof in oral cavity |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6117409A (en) * | 1984-07-02 | 1986-01-25 | Meishin Toryo Kk | Preparation of amorphous calcium phosphate and bio-compatible composition composed mainly thereof |
| WO2013034421A2 (en) * | 2011-09-08 | 2013-03-14 | Unilever N.V. | Tooth remineralizing dentifrice |
| CN103027845A (en) * | 2013-01-06 | 2013-04-10 | 浙江大学 | Method for repairing demineralized dentin |
| CN104555966A (en) * | 2014-12-10 | 2015-04-29 | 华南理工大学 | Organic biomolecular composite calcium phosphate nanoparticles and preparation method thereof |
| CN104983583A (en) * | 2015-07-27 | 2015-10-21 | 天津医科大学口腔医院 | Directional orderly mineralized material for tooth enamel and preparation method thereof |
-
2015
- 2015-11-02 CN CN201510730463.8A patent/CN105267046B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6117409A (en) * | 1984-07-02 | 1986-01-25 | Meishin Toryo Kk | Preparation of amorphous calcium phosphate and bio-compatible composition composed mainly thereof |
| WO2013034421A2 (en) * | 2011-09-08 | 2013-03-14 | Unilever N.V. | Tooth remineralizing dentifrice |
| CN103027845A (en) * | 2013-01-06 | 2013-04-10 | 浙江大学 | Method for repairing demineralized dentin |
| CN104555966A (en) * | 2014-12-10 | 2015-04-29 | 华南理工大学 | Organic biomolecular composite calcium phosphate nanoparticles and preparation method thereof |
| CN104983583A (en) * | 2015-07-27 | 2015-10-21 | 天津医科大学口腔医院 | Directional orderly mineralized material for tooth enamel and preparation method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4023204A1 (en) * | 2020-12-30 | 2022-07-06 | Stick Tech OY | A kit of parts for dental restoration |
| WO2022144492A1 (en) * | 2020-12-30 | 2022-07-07 | Stick Tech Oy | A kit of parts for dental restoration |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105267046A (en) | 2016-01-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105267046B (en) | A kind of quick method for repairing demineralization dentine | |
| Busch et al. | Morphogenesis and structure of human teeth in relation to biomimetically grown fluorapatite− gelatine composites | |
| Parker et al. | Measurement of the efficacy of calcium silicate for the protection and repair of dental enamel | |
| Niu et al. | Biomimetic remineralization of dentin | |
| Chen et al. | Acellular synthesis of a human enamel-like microstructure | |
| Wang et al. | Calcium orthophosphates: crystallization and dissolution | |
| Zhang et al. | Synthesis of dental enamel-like hydroxyapaptite through solution mediated solid-state conversion | |
| Adams et al. | Bioactive glass 45S5 from diatom biosilica | |
| CN106473933A (en) | The biomineralization material of bonding auxiliary and its application in biomimetic mineralization | |
| CN102631702B (en) | The complex of mineralized collagen and bioceramic and manufacture method thereof | |
| CN109718249A (en) | A kind of calcium phosphate nano bunch and its preparation method and application | |
| CN101786615B (en) | Hydroxyapatite nanotube and preparation and application thereof in bone repair | |
| Clift | Artificial methods for the remineralization of hydroxyapatite in enamel | |
| CN103520779A (en) | Porous composite biological material containing multiple active ions and preparation method thereof | |
| CN103027845A (en) | Method for repairing demineralized dentin | |
| CN106074184B (en) | A kind of remineralization material and preparation method thereof for demineralization enamel surface | |
| CN104983583B (en) | One kind is used for oriented and ordered mineralizing material of enamel and preparation method thereof | |
| CN104163622B (en) | Preparation method and application of laponite bioceramics | |
| Jia et al. | Adhesion of Streptococcus mutans on remineralized enamel surface induced by poly (amido amine) dendrimers | |
| CN106432420A (en) | Polypeptide modified tannic acid and preparation method and application thereof | |
| CN105497986A (en) | Synthesis method of graphene-hydroxyapatite composite material | |
| CN102491300A (en) | Preparation method of amphiphilic monodisperse hydroxyapatite monocrystal nanorod | |
| Hanafy et al. | Biomimetic chitosan against bioinspired nanohydroxyapatite for repairing enamel surfaces | |
| Koroleva et al. | Biomaterial based on doped calcium carbonate-phosphate for active osteogenesis | |
| Qin et al. | Facet-specific dissolution–precipitation at struvite–water interfaces |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |