CN105259241A - Multi-V-shaped pipe sample applicator for protein running gel - Google Patents
Multi-V-shaped pipe sample applicator for protein running gel Download PDFInfo
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- CN105259241A CN105259241A CN201510737574.1A CN201510737574A CN105259241A CN 105259241 A CN105259241 A CN 105259241A CN 201510737574 A CN201510737574 A CN 201510737574A CN 105259241 A CN105259241 A CN 105259241A
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Abstract
The invention discloses a multi-V-shaped pipe sample applicator for protein running gel. The multi-V-shaped pipe sample applicator is characterized by comprising a plurality of V-shaped pipes matched with SDS-PAGE (Sodium Dodecyl Sulfate -Polyacrylamide Gel Electrophoresis) film combed tooth sockets in quantity; the plurality of V-shaped pipes are communicated by a horizontal connecting pipe; a sample injection hole is formed at the V-shaped bottom of each V-shaped pipe; a sample adding inlet is formed in a communicating part of one side of a V-shaped opening of each V-shaped pipe and the horizontal connecting pipe; during sample addition, various V-shaped pipes are enabled to respectively align to corresponding combed tooth sockets of the SDS-PAGE film, and the V-shaped bottom of each V-shaped pipe extends into one corresponding combed tooth socket and is positioned.
Description
Technical field
The present invention relates to a kind of molecular biology experiment technology, particularly a kind of SDS-PAGE (polyacrylamide gel electrophoresis) runs the application of sample apparatus of protein sample in glue operating process.
Background technology
In molecular biology experiment technical field, modal is Westernblot experiment (western blot test), and whole experimentation comprises---manufacture SDS-PAGE film, loading, electrophoresis, transferring film, close, add antibody, luminescence etc.In glue loading process, the concentration of glue depends on destination protein molecular weight analyte size, and the size of applied sample amount depends on that the speed that the result of protein quantification, sample protein are run is by the pH value of electrolytic solution, and voltage and gravity determine jointly.When the specific electrolytic solution of use, and when maintaining relative constancy voltage, the speed that sample protein declines depends primarily on size and the weight (namely the larger race of molecular weight is slower) of protein molecular, the order of accuarcy of applied sample amount directly affects electrophoretic effects, therefore, glue and loading are most important in electrophoresis process.Current glue technology is mature technology, and loading process adopts sample loading gun that sample one lattice one lattice of different albumen are expelled to (feeding chamber in Fig. 1) in the teeth groove of pectination SDS-PAGE film 4.But, often there is following problem in use: 1. rifle head or syringe needle easily puncture gel, directly affect quality of experiments; 2. application of sample can make sample go out feeding chamber 2 too soon, if there is bubble that sample also may be made to overflow; 3. easily mix mutually between different sample between different feeding chamber, cannot cross pollution be avoided.Unnecessary error is caused to Coupon Testing Results.
Summary of the invention
The object of this invention is to provide that a kind of structure is simple, loading is accurate, convenient and time-saving, substantially can meet the race glue application of sample utensil that Westernblot tests various protein sample demand.
For reaching above object, the present invention takes following technical scheme to be achieved:
Run many V-arrangements pipe injector of glue for albumen, it is characterized in that, comprise the multiple V-arrangement pipes supporting with SDS-PAGE film pectination teeth groove quantity, be communicated with by horizontal connecting leg between the plurality of V-arrangement pipe, at the bottom of the V-arrangement of each V-arrangement pipe, be equipped with a note sample hole; V-arrangement opening side and the horizontal connecting leg connectivity part of each V-arrangement pipe are equipped with an adding mouth, during application of sample, each V-arrangement pipe are aimed at respectively the corresponding pectination teeth groove of SDS-PAGE film, all will to put in a pectination teeth groove and located at the bottom of each V-arrangement pipe V-arrangement.
In such scheme, in described multiple V-arrangement pipe, high order end is connected a ram's horn respectively with the V-arrangement pipe of low order end, these two symmetrical distributions of ram's horn, when two ram's horns are symmetrical be fixed on run on glue sampling device left and right sides column time, by all to put at the bottom of each V-arrangement pipe V-arrangement in a pectination teeth groove and to be located.
Described V-arrangement pipe and horizontal connecting leg are made up of stainless steel or organic material, and external diameter is not more than 0.75mm.
Described adding mouth is a funnel.This funnel can dock with 10 microlitre sample loading gun heads.
Compared with prior art, conveniently moving of the present invention, accurate positioning, rational in infrastructure, and, facilitate, save time, economy, science, meet the demand of current various race glue loading.
Accompanying drawing explanation
Fig. 1 is SDS-PAGE film structure figure in existing race glue sampling device.
Fig. 2 is erietiform injector structural representation of the present invention.
Fig. 3 is I enlarged drawing in Fig. 2.
Fig. 4 is the schematic diagram of SDS-PAGE film pectination teeth groove Fig. 2 injector being placed in Fig. 1 device.
In Fig. 1 ~ Fig. 4: 1, electrode terminal; 2, feeding chamber; 3, rear plate plane; 4, SDS-PAGE film; 5, front plate plane; 6, column; 7, horizontal connecting leg; 8, V-arrangement pipe; 9, adding mouth; 10, ram's horn; 11, funnel; 12, sample hole is noted.
Embodiment
With reference to figure 1, in existing race glue sampling device, adopt forward and backward clamping plate (transparent glass sheet, not shown in FIG.) in the gap that it is parallel, first prepare SDS-PAGE film 4, insert the mould of a pectination in film in forward and backward clamping plate upper end before solidifying, after film solidifies, extract pectination mould, thus make to form multiple teeth groove between film upper end and forward and backward clamping plate plane 5,3, be also feeding chamber 2.Existing loading methods adopts sample loading gun (entry needle) to be expelled in different feeding chambers respectively by the sample of different albumen, as described in background technology, there is many defects.
With reference to figure 2, Fig. 4, the present invention devises a kind of many V-arrangements pipe injector, comprise the multiple V-arrangement pipes 8 supporting with SDS-PAGE film 4 teeth groove, be communicated with by horizontal connecting leg 7 between multiple V-arrangement pipe, each V-arrangement pipe V-arrangement opening side and horizontal connecting leg connectivity part are equipped with an adding mouth 9, V-arrangement pipe and horizontal connecting leg are made up of stainless steel or organic material, and external diameter is not more than 0.75mm.In the structure of multiple V-arrangement pipe 8 be communicated with by horizontal connecting leg, the V-arrangement pipe of high order end connects a ram's horn 10, and the V-arrangement pipe of low order end connects another ram's horn 10, these two symmetrical distributions of ram's horn.During application of sample, each V-arrangement pipe of Fig. 2 erietiform injector is aimed at respectively the corresponding pectination teeth groove (Fig. 4) of SDS-PAGE film 4, two ram's horns are hung on the upper end that Fig. 1 runs the corresponding column 6 of glue sampling device respectively, thus make each V-arrangement Guan Jun of injector stretch into a feeding chamber 2 and be located.
With reference to figure 3, adding mouth 9 is a funnel 11, and this funnel can dock with 10 microlitre sample loading gun heads, and when can avoid application of sample, sample leaks outside.Be equipped with a note sample hole 12 at the bottom of each V-arrangement pipe V-arrangement, sample can accurately add in corresponding feeding chamber (track) by it, overcomes the maloperation that in the past clearly cannot distinguish swimming lane.
This note sample hole is also communicated with the adding mouth of adjacent V-arrangement pipe outside being communicated with the adding mouth 9 of this V-arrangement pipe, like this, can prevent back flow of sample on the one hand, the bubble added in feeding chamber sample can be discharged on the other hand, prevent the pollution to adjacent feeding chamber sample.
Claims (4)
1. run many V-arrangements pipe injector of glue for albumen, it is characterized in that, comprise the multiple V-arrangement pipes supporting with SDS-PAGE film pectination teeth groove quantity, be communicated with by horizontal connecting leg between the plurality of V-arrangement pipe, at the bottom of the V-arrangement of each V-arrangement pipe, be equipped with a note sample hole; V-arrangement opening side and the horizontal connecting leg connectivity part of each V-arrangement pipe are equipped with an adding mouth, during application of sample, each V-arrangement pipe are aimed at respectively the corresponding pectination teeth groove of SDS-PAGE film, all will to put in a pectination teeth groove and located at the bottom of each V-arrangement pipe V-arrangement.
2. many V-arrangements pipe injector of glue is run as claimed in claim 1 for albumen, it is characterized in that, in described multiple V-arrangement pipe, high order end is connected a ram's horn respectively with the V-arrangement pipe of low order end, these two symmetrical distributions of ram's horn, when two ram's horns are symmetrical be fixed on run on glue sampling device left and right sides column time, by all to put at the bottom of each V-arrangement pipe V-arrangement in a pectination teeth groove and to be located.
3. run many V-arrangements pipe injector of glue as claimed in claim 1 for albumen, it is characterized in that, described V-arrangement pipe and horizontal connecting leg are made up of stainless steel or organic material, and external diameter is not more than 0.75mm.
4. run many V-arrangements pipe injector of glue as claimed in claim 1 for albumen, it is characterized in that, described adding mouth is a funnel, and this funnel can dock with 10 microlitre sample loading gun heads.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510737574.1A CN105259241B (en) | 2015-11-02 | 2015-11-02 | A kind of more V-arrangement pipe injectors that glue is run for albumen |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510737574.1A CN105259241B (en) | 2015-11-02 | 2015-11-02 | A kind of more V-arrangement pipe injectors that glue is run for albumen |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105259241A true CN105259241A (en) | 2016-01-20 |
| CN105259241B CN105259241B (en) | 2018-01-30 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510737574.1A Expired - Fee Related CN105259241B (en) | 2015-11-02 | 2015-11-02 | A kind of more V-arrangement pipe injectors that glue is run for albumen |
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| CN (1) | CN105259241B (en) |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5217591A (en) * | 1990-05-14 | 1993-06-08 | Labintelligence, Inc. | Gel electrophoresis sample applicator/retriever |
| US5304292A (en) * | 1992-10-29 | 1994-04-19 | Jule, Inc. | Electrophoresis gels |
| WO2002042758A1 (en) * | 2000-11-27 | 2002-05-30 | Large Scale Proteomics Corporation | Method and device for forming wells in an electrophoresis gel slab |
| CN201004058Y (en) * | 2007-01-09 | 2008-01-09 | 山西大学 | Novel sampling gun group for electrophoresis experiment |
| CN103033551A (en) * | 2012-12-03 | 2013-04-10 | 杭州吉来生物技术有限公司 | Sample loading guide for gel electrophoresis system |
| WO2014128956A1 (en) * | 2013-02-25 | 2014-08-28 | 株式会社島津製作所 | Capillary unit for electrophoresis and electrophoresis device comprising same capillary unit |
| CN203849203U (en) * | 2014-05-27 | 2014-09-24 | 李水清 | Sampler for agarose gel electrophoresis |
| CN204154730U (en) * | 2014-09-05 | 2015-02-11 | 黑龙江省恒生干细胞工程有限公司 | A kind of Multi needle microsyringe |
| CN205175959U (en) * | 2015-11-02 | 2016-04-20 | 中国人民解放军第四军医大学 | A many V -arrangements pipe is an appearance ware upward for albumen runs glues |
-
2015
- 2015-11-02 CN CN201510737574.1A patent/CN105259241B/en not_active Expired - Fee Related
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5217591A (en) * | 1990-05-14 | 1993-06-08 | Labintelligence, Inc. | Gel electrophoresis sample applicator/retriever |
| US5304292A (en) * | 1992-10-29 | 1994-04-19 | Jule, Inc. | Electrophoresis gels |
| WO2002042758A1 (en) * | 2000-11-27 | 2002-05-30 | Large Scale Proteomics Corporation | Method and device for forming wells in an electrophoresis gel slab |
| CN201004058Y (en) * | 2007-01-09 | 2008-01-09 | 山西大学 | Novel sampling gun group for electrophoresis experiment |
| CN103033551A (en) * | 2012-12-03 | 2013-04-10 | 杭州吉来生物技术有限公司 | Sample loading guide for gel electrophoresis system |
| WO2014128956A1 (en) * | 2013-02-25 | 2014-08-28 | 株式会社島津製作所 | Capillary unit for electrophoresis and electrophoresis device comprising same capillary unit |
| CN203849203U (en) * | 2014-05-27 | 2014-09-24 | 李水清 | Sampler for agarose gel electrophoresis |
| CN204154730U (en) * | 2014-09-05 | 2015-02-11 | 黑龙江省恒生干细胞工程有限公司 | A kind of Multi needle microsyringe |
| CN205175959U (en) * | 2015-11-02 | 2016-04-20 | 中国人民解放军第四军医大学 | A many V -arrangements pipe is an appearance ware upward for albumen runs glues |
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| Publication number | Publication date |
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| CN105259241B (en) | 2018-01-30 |
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Granted publication date: 20180130 Termination date: 20181102 |