CN105254037A - Method for improving activity of microorganisms in nano-silver-containing water body - Google Patents
Method for improving activity of microorganisms in nano-silver-containing water body Download PDFInfo
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- CN105254037A CN105254037A CN201510840027.6A CN201510840027A CN105254037A CN 105254037 A CN105254037 A CN 105254037A CN 201510840027 A CN201510840027 A CN 201510840027A CN 105254037 A CN105254037 A CN 105254037A
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Abstract
The invention discloses a method for improving the activity of microorganisms in a nano-silver-containing water body. The method includes the following steps that organisms and the nano-silver-containing water body are mixed, and mixed liquid is obtained; the microorganisms are added to be subjected to shake cultivation, and treatment of the microorganisms is completed. The method is easy and convenient to operate and high in practicability, damage of nano-silver in the water body to the microorganisms can be effectively suppressed, the cell activity of the microorganisms in the nano-silver-containing water body environment can be improved, and the method is of great significance in practical application to treating nano-silver-containing wastewater.
Description
Technical field
The invention belongs to nano-Ag particles to beneficial microorganism toxic action Application Areas, relate to a kind of method improved containing microorganism active in nanometer silver water body, be specifically related to a kind of organism that utilizes and improve the method containing microorganism active in nanometer silver water body.
Background technology
Nanometer silver (Silvernanoparticles) has good sterilization effect and the performance such as catalysis, photoelectricity, and size range is between 1 ~ 100nm.A large amount of uses of nanometer silver, make nanometer silver constantly be released in environment, add its potential risk to various biology, especially beneficial microorganism.After nanometer silver enters water surrounding, the impact of various factors can be subject to, comprise pH, temperature, dissolved oxygen, organism etc., the existence of these conditions or material can have a huge impact, thus affect the toxicity of nanometer silver to microorganism the migration of nanometer silver, conversion.
For Phanerochaete chrysosporium, Phanerochaete chrysosporium is widely applied in heavy metal process and composting technology, but when utilizing Phanerochaete chrysosporium process to contain the water body of nanometer silver, due to the toxic action of nanometer silver, make Phanerochaete chrysosporium receive damage to a certain degree, serious affect its processing efficiency to pollutent.
In view of the widespread use of nanometer silver, in actual environment, the exposed amount of nanometer silver also increases thereupon, therefore, improves beneficial microorganism active significant containing nanometer silver waste water glassware for drinking water to process.But in actual applications, lack the effective technology means improved containing microorganism active in nanometer silver waste water.
Summary of the invention
The technical problem to be solved in the present invention overcomes deficiency of the prior art, provide a kind of simple to operation, economical, feasible, practical, do not produce the method for other raisings of polluting containing microorganism active in nanometer silver water body.The method effectively reduces containing nanometer silver in nanometer silver waste water to the toxic action of microorganism by adding organism, thus improves containing microbial cell in nanometer silver waste water active, and then improves the efficiency of microbiological treatment pollutent.
For solving the problems of the technologies described above, the technical solution used in the present invention is:
Improve the method containing microorganism active in nanometer silver water body, comprise the following steps: by organism and containing nanometer silver water mixing, obtain mixed solution; Add microorganism and carry out shaking culture, complete the process to microorganism.
In above-mentioned method, preferably, described organism comprises one or more in halfcystine, humic acid, bovine serum albumin.
In above-mentioned method, preferably, in described mixed solution, the concentration of halfcystine is 1mg/L ~ 100mg/L.
In above-mentioned method, preferably, in described mixed solution, the concentration of humic acid is 1mg/L ~ 100mg/L.
In above-mentioned method, further preferably, in described mixed solution, the concentration of humic acid is 50mg/L ~ 100mg/L.
In above-mentioned method, preferably, in described mixed solution, the concentration of bovine serum albumin is 1mg/L ~ 50mg/L.
In above-mentioned method, preferably, described is 0.001mg/L ~ 2mg/L containing the concentration of nanometer silver in nanometer silver water body.
In above-mentioned method, preferably, described microorganism is Phanerochaete chrysosporium, blue-green algae or nitrobacteria.
In above-mentioned method, preferably, the temperature of described shaking culture is 36 DEG C ~ 38 DEG C, and rotating speed is 120rpm ~ 150rpm, and the time is 10h ~ 24h.
Innovative point of the present invention is:
The present invention utilizes organism to mix with nanometer silver first, to be used for controlling in water body nanometer silver to the toxic effect of Phanerochaete chrysosporium.By adding organism, interact with nanometer silver, the stability of nanometer silver is changed, affect nanometer silver state in the solution, thus effectively reduce nanometer silver to the toxic action of Phanerochaete chrysosporium, containing the activity in nanometer silver water body environment to improve Phanerochaete chrysosporium.
Organism is extensively exist in natural water body, but the interaction of it and nanometer silver is also not very clear at present.In the present invention, organism can not only change surface charge and their stability of nanometer silver, can also be combined with the silver ions that nanometer silver discharges formation title complex, wherein organism can be combined by the silver ions in Nano silver solution, reduce nanometer silver to the toxicity of microbial cells, and organism also can with nanometer silver surface bonding, reduce nanometer silver release silver ions.For organism, different organism has different structures and composition, and these differences can produce different impacts to the effect between nanometer silver from Phanerochaete chrysosporium.Organism in the present invention comprise in halfcystine, humic acid, bovine serum albumin one or more, these three kinds of organism have different groups, and these groups have different impacts to nanometer silver, as sulfydryl, amino groups effectively in conjunction with silver ions, thus can affect the toxicity of nanometer silver.
Compared with prior art, the invention has the advantages that:
1, the invention provides a kind of method improved containing microorganism active in nanometer silver water body, by adding organism, can effectively suppress nanometer silver to the toxic action of microorganism, containing the activity in nanometer silver water body environment to improve microorganism.By controlling organic concentration, microorganism can be provided to contain the optimum growh state in nanometer silver water body environment.
2, method of the present invention is simple to operation, selected organism is widespread in nature, easy acquisition, therefore the present invention have economy, feasible, practical, do not produce the features such as other pollutions, effectively can reduce the harm of nanometer silver to microorganism, improve microbial cell in water body active, and then the efficiency improved containing microbiological treatment pollutent in nanometer silver water body, the antimicrobial treatment process containing nanometer silver water body can be widely used in, be significant in process is containing the application of nanometer silver water body.
Accompanying drawing explanation
For making the object of the embodiment of the present invention, technical scheme and advantage clearly, below in conjunction with the accompanying drawing in the embodiment of the present invention, clear, complete description is carried out to the technical scheme in the embodiment of the present invention.
Fig. 1 is the cytoactive schematic diagram of Phanerochaete chrysosporium in the mixed solution of the different semicystinol concentrations of the embodiment of the present invention 1.
Fig. 2 is the cytoactive schematic diagram of Phanerochaete chrysosporium in the mixed solution of the different humic acid concentration of the embodiment of the present invention 2.
Fig. 3 is the cytoactive schematic diagram of Phanerochaete chrysosporium in the mixed solution of the different bovine serum albumin concentration of the embodiment of the present invention 3.
Embodiment
Below in conjunction with Figure of description and concrete preferred embodiment, the invention will be further described, but protection domain not thereby limiting the invention.
The material adopted in following examples and instrument are commercially available.
embodiment 1
Raising of the present invention is containing a method for microorganism active in nanometer silver water body, and wherein microorganism is Phanerochaete chrysosporium, comprises the following steps:
(1) prepare Phanerochaete chrysosporium bacterium ball: be inoculated in by Phanerochaete chrysosporium BKM-F1767 in Kirk substratum, at 37 DEG C of temperature, cultivate 72h with 120rpm speed oscillation, obtain Phanerochaete chrysosporium bacterium ball.
(2) halfcystine is got respectively, with the concentration of nanometer silver be 1mg/L containing nanometer silver water mixing even, obtain the mixed solution of different semicystinol concentration, wherein in mixed solution, the concentration of halfcystine is respectively 0.1mg/L, 1mg/L, 5mg/L, 10mg/L, 20mg/L, 50mg/L, 100mg/L.
(3) the Phanerochaete chrysosporium bacterium ball in 0.2g step (1) is got respectively, join in the mixed solution of the different semicystinol concentrations of 5mL step (2), at temperature is 37 DEG C, cultivate 12 hours with 120rpm speed oscillation, complete the process to Phanerochaete chrysosporium.
Control group: compared with the present embodiment 1, difference is only: do not add halfcystine.
Fig. 1 is the cytoactive schematic diagram of Phanerochaete chrysosporium in the mixed solution of the different semicystinol concentration of the present embodiment.As shown in Figure 1, containing adding after concentration is the halfcystine of 1 ~ 100mg/L mixed solution in nanometer silver water body, can effectively to suppress in water body nanometer silver to the toxic action of Phanerochaete chrysosporium, significantly improve Phanerochaete chrysosporium and contain the activity in nanometer silver water body, and along with the increase of semicystinol concentration, the cytoactive of Phanerochaete chrysosporium also increases accordingly.When semicystinol concentration is too low, the cytoactive of Phanerochaete chrysosporium can decline to some extent.
embodiment 2
Raising of the present invention is containing a method for microorganism active in nanometer silver water body, and wherein microorganism is Phanerochaete chrysosporium, comprises the following steps:
(1) Phanerochaete chrysosporium bacterium ball is prepared: with embodiment 1.
(2) humic acid is got respectively, with the concentration of nanometer silver be 1mg/L containing nanometer silver water mixing even, obtain the mixed solution of different humic acid concentration, wherein in mixed solution, the concentration of humic acid is respectively 1mg/L, 2mg/L, 5mg/L, 10mg/L, 20mg/L, 50mg/L, 100mg/L.
(3) the Phanerochaete chrysosporium bacterium ball in 0.2g step (1) is got respectively, join in the mixed solution of different humic acid concentration of 5mL step (2), at temperature is 37 DEG C, cultivate 12 hours with the speed oscillation of 120rpm, complete the process to Phanerochaete chrysosporium.
Control group: compared with the present embodiment 2, difference is only: do not add humic acid.
Fig. 2 is the cytoactive schematic diagram of Phanerochaete chrysosporium in the mixed solution of the different humic acid concentration of the present embodiment.As shown in Figure 2, containing adding after concentration is the humic acid of 1 ~ 100mg/L mixed solution in nanometer silver water body, Phanerochaete chrysosporium can be improved to a certain extent containing the activity in nanometer silver water body, and along with the increase of humic acid concentration, the cytoactive raising among a small circle of Phanerochaete chrysosporium.
embodiment 3
Raising of the present invention is containing a method for microorganism active in nanometer silver water body, and wherein microorganism is Phanerochaete chrysosporium, comprises the following steps:
(1) Phanerochaete chrysosporium bacterium ball is prepared: with embodiment 1.
(2) bovine serum albumin is got respectively, with the concentration of nanometer silver be 1mg/L containing nanometer silver water mixing even, obtain the mixed solution of different bovine serum albumin concentration, wherein in mixed solution, the concentration of bovine serum albumin is respectively 1mg/L, 5mg/L, 10mg/L, 50mg/L, 100mg/L, 200mg/L, 500mg/L.
(3) the Phanerochaete chrysosporium bacterium ball in 0.2g step (1) is got respectively, join in the mixed solution of different bovine serum albumin concentration of 5mL step (2), at temperature is 37 DEG C, cultivate 12 hours with the speed oscillation of 120rpm, complete the process to Phanerochaete chrysosporium.
Control group: compared with the present embodiment 3, difference is only: do not add bovine serum albumin.
Fig. 3 is the cytoactive schematic diagram of Phanerochaete chrysosporium in the mixed solution of the different bovine serum albumin concentration of the present embodiment.As shown in Figure 3, the concentration of bovine serum albumin is when 1 ~ 50mg/L mixed solution, and Phanerochaete chrysosporium is being improved containing the activity in nanometer silver water body, and wherein when 5mg/L, cytoactive is the highest, and when the concentration of bovine serum albumin is higher than 50mg/L, cytoactive declines.Therefore, control the concentration of bovine serum albumin, effectively can control the toxicity of nanometer silver to Phanerochaete chrysosporium, wherein when the concentration of bovine serum albumin is 5mg/L, cytoactive is the highest, toxicity inhibition best results.
The present invention, by adding organism, can effectively reduce the toxic action of nanometer silver to Phanerochaete chrysosporium, is containing the activity in nanometer silver waste water to improve Phanerochaete chrysosporium; By controlling organic concentration, effectively can control the toxic action of nanometer silver to Phanerochaete chrysosporium, to determine that Phanerochaete chrysosporium is containing the optimum growh state in nanometer silver waste water.The inventive method is simple to operation, economical, feasible, practical, do not produce other pollutes, effectively can reduce the harm of nanometer silver to Phanerochaete chrysosporium, improve Phanerochaete chrysosporium and contain the cytoactive in nanometer silver water body, and then improve the efficiency of Phanerochaete chrysosporium process pollutent, the Phanerochaete chrysosporium treating processes containing nanometer silver waste water can be widely used in, in process is containing the practical application of nanometer silver waste water, there is great practical significance.
The above is only the preferred embodiment of the present invention, and protection scope of the present invention is also not only confined to above-described embodiment.All technical schemes belonged under thinking of the present invention all belong to protection scope of the present invention.It is noted that for those skilled in the art, improvements and modifications under the premise without departing from the principles of the invention, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (9)
1. improve the method containing microorganism active in nanometer silver water body, it is characterized in that, comprise the following steps: by organism and containing nanometer silver water mixing, obtain mixed solution; Add microorganism and carry out shaking culture, complete the process to microorganism.
2. method according to claim 1, is characterized in that, described organism comprise in halfcystine, humic acid, bovine serum albumin one or more.
3. method according to claim 2, is characterized in that, in described mixed solution, the concentration of halfcystine is 1mg/L ~ 100mg/L.
4. method according to claim 2, is characterized in that, in described mixed solution, the concentration of humic acid is 1mg/L ~ 100mg/L.
5. method according to claim 4, is characterized in that, in described mixed solution, the concentration of humic acid is 50mg/L ~ 100mg/L.
6. method according to claim 2, is characterized in that, in described mixed solution, the concentration of bovine serum albumin is 1mg/L ~ 50mg/L.
7. method according to any one of claim 1 to 6, is characterized in that, described is 0.001mg/L ~ 2mg/L containing the concentration of nanometer silver in nanometer silver water body.
8. method according to any one of claim 1 to 6, is characterized in that, described microorganism is Phanerochaete chrysosporium, blue-green algae or nitrobacteria.
9. method according to any one of claim 1 to 6, is characterized in that, the temperature of described shaking culture is 36 DEG C ~ 38 DEG C, and rotating speed is 120rpm ~ 150rpm, and the time is 10h ~ 24h.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106248451A (en) * | 2016-07-08 | 2016-12-21 | 湖南大学 | The method improving river sand Heavy Metal In Sediments dissolution |
| CN106747734A (en) * | 2016-12-29 | 2017-05-31 | 湖南大学 | The method that nitrogen loss in composting process is reduced using Nano Silver |
| CN108911050A (en) * | 2018-07-26 | 2018-11-30 | 湖南大学 | A method of reducing nano silver toxicity |
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| CN104193012A (en) * | 2014-09-11 | 2014-12-10 | 湖南大学 | Method for promoting phanerochaete chrysosporium to remove heavy metal cadmium by utilizing silver nanoparticles |
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2015
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| CN104193012A (en) * | 2014-09-11 | 2014-12-10 | 湖南大学 | Method for promoting phanerochaete chrysosporium to remove heavy metal cadmium by utilizing silver nanoparticles |
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| ANN-KATHRIN OSTERMEYER ET AL.: ""Influence of Bovine Serum Albumin and Alginate on Silver Nanoparticle Dissolution and Toxicity to Nitrosomonas europaea"", 《ENVIRONMENTAL SCIENCE & TECHNOLOGY》 * |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106248451A (en) * | 2016-07-08 | 2016-12-21 | 湖南大学 | The method improving river sand Heavy Metal In Sediments dissolution |
| CN106248451B (en) * | 2016-07-08 | 2018-12-18 | 湖南大学 | The method for improving the dissolution of river sand Heavy Metal In Sediments |
| CN106747734A (en) * | 2016-12-29 | 2017-05-31 | 湖南大学 | The method that nitrogen loss in composting process is reduced using Nano Silver |
| CN108911050A (en) * | 2018-07-26 | 2018-11-30 | 湖南大学 | A method of reducing nano silver toxicity |
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