CN105247076A - 使用拼装序列扩增片段化的目标核酸的方法 - Google Patents
使用拼装序列扩增片段化的目标核酸的方法 Download PDFInfo
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- CN105247076A CN105247076A CN201480027411.8A CN201480027411A CN105247076A CN 105247076 A CN105247076 A CN 105247076A CN 201480027411 A CN201480027411 A CN 201480027411A CN 105247076 A CN105247076 A CN 105247076A
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- nucleic acid
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
Claims (3)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201361798984P | 2013-03-15 | 2013-03-15 | |
US61/798,984 | 2013-03-15 | ||
PCT/US2014/029893 WO2014145176A1 (en) | 2013-03-15 | 2014-03-15 | Methods for amplifying fragmented target nucleic acids utilizing an assembler sequence |
Publications (2)
Publication Number | Publication Date |
---|---|
CN105247076A true CN105247076A (zh) | 2016-01-13 |
CN105247076B CN105247076B (zh) | 2021-06-18 |
Family
ID=51537894
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201480027411.8A Active CN105247076B (zh) | 2013-03-15 | 2014-03-15 | 使用拼装序列扩增片段化的目标核酸的方法 |
Country Status (6)
Country | Link |
---|---|
EP (1) | EP2971134B1 (zh) |
JP (2) | JP2016512696A (zh) |
CN (1) | CN105247076B (zh) |
CA (1) | CA2904863C (zh) |
HK (1) | HK1219117A1 (zh) |
WO (1) | WO2014145176A1 (zh) |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5871911A (en) * | 1992-12-07 | 1999-02-16 | Wisconsin Alumni Research Foundation | Method of site-specific nucleic acid cleavage |
US20060057567A1 (en) * | 2001-05-23 | 2006-03-16 | Hong Soon G | Method for constructing a chimeric dna library using a single strand speific dnase |
US20100151477A1 (en) * | 2002-01-31 | 2010-06-17 | Richard Cawthon | Reducing non-target nucleic acid dependent amplifications: amplifying repetitive nucleic acid sequences |
WO2010080910A1 (en) * | 2009-01-08 | 2010-07-15 | Bio-Rad Laboratories, Inc. | Methods and compositions for improving efficiency of nucleic acids amplification reactions |
CN101889096A (zh) * | 2007-10-04 | 2010-11-17 | 联邦科学及工业研究组织 | 核酸扩增 |
Family Cites Families (14)
Publication number | Priority date | Publication date | Assignee | Title |
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US5585481A (en) | 1987-09-21 | 1996-12-17 | Gen-Probe Incorporated | Linking reagents for nucleotide probes |
US5378825A (en) | 1990-07-27 | 1995-01-03 | Isis Pharmaceuticals, Inc. | Backbone modified oligonucleotide analogs |
ATE515510T1 (de) | 1991-12-24 | 2011-07-15 | Isis Pharmaceuticals Inc | Durch dna-abschnitte unterbrochene modifizierte oligonukleotide |
US5928905A (en) * | 1995-04-18 | 1999-07-27 | Glaxo Group Limited | End-complementary polymerase reaction |
US6169169B1 (en) | 1994-05-19 | 2001-01-02 | Dako A/S | PNA probes for detection of Neisseria gonorrhoeae and Chlamydia trachomatis |
US6153410A (en) * | 1997-03-25 | 2000-11-28 | California Institute Of Technology | Recombination of polynucleotide sequences using random or defined primers |
US6949367B1 (en) | 1998-04-03 | 2005-09-27 | Epoch Pharmaceuticals, Inc. | Modified oligonucleotides for mismatch discrimination |
WO2002086121A1 (en) * | 2001-04-25 | 2002-10-31 | Proteus | Template-mediated, ligation-oriented method of nonrandomly shuffling polynucleotides |
US20040241732A1 (en) * | 2003-04-11 | 2004-12-02 | Chu-An Chang | Method of generating long nucleic acid molecules of defined sequence |
US6878531B1 (en) * | 2003-11-10 | 2005-04-12 | Medical College Of Georgia Research Institute | Method for multiple site-directed mutagenesis |
GB0500703D0 (en) * | 2005-01-14 | 2005-02-23 | Bioinvent Int Ab | Molecular biology method |
EP2430180A4 (en) * | 2009-05-11 | 2012-11-07 | Agency Science Tech & Res | gene synthesis |
CN101899434A (zh) * | 2010-06-18 | 2010-12-01 | 南方医科大学 | 一种可连接多片段的重叠延伸pcr方法 |
KR102252447B1 (ko) | 2011-05-04 | 2021-05-17 | 바이오셉트 인코포레이티드 | 핵산 서열 변이체를 검출하는 방법 |
-
2014
- 2014-03-15 JP JP2016503272A patent/JP2016512696A/ja active Pending
- 2014-03-15 EP EP14765053.5A patent/EP2971134B1/en active Active
- 2014-03-15 CN CN201480027411.8A patent/CN105247076B/zh active Active
- 2014-03-15 WO PCT/US2014/029893 patent/WO2014145176A1/en active Application Filing
- 2014-03-15 CA CA2904863A patent/CA2904863C/en active Active
-
2016
- 2016-06-21 HK HK16107182.5A patent/HK1219117A1/zh unknown
-
2019
- 2019-05-22 JP JP2019095840A patent/JP2019176860A/ja active Pending
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5871911A (en) * | 1992-12-07 | 1999-02-16 | Wisconsin Alumni Research Foundation | Method of site-specific nucleic acid cleavage |
US20060057567A1 (en) * | 2001-05-23 | 2006-03-16 | Hong Soon G | Method for constructing a chimeric dna library using a single strand speific dnase |
US20100151477A1 (en) * | 2002-01-31 | 2010-06-17 | Richard Cawthon | Reducing non-target nucleic acid dependent amplifications: amplifying repetitive nucleic acid sequences |
CN101889096A (zh) * | 2007-10-04 | 2010-11-17 | 联邦科学及工业研究组织 | 核酸扩增 |
WO2010080910A1 (en) * | 2009-01-08 | 2010-07-15 | Bio-Rad Laboratories, Inc. | Methods and compositions for improving efficiency of nucleic acids amplification reactions |
Non-Patent Citations (1)
Title |
---|
I NASIDZE ET AL.: "Construction of Larger- Size Sequencing Templates from Degraded DNA", 《BIOTECHNIQUES》 * |
Also Published As
Publication number | Publication date |
---|---|
CA2904863C (en) | 2022-12-13 |
EP2971134A4 (en) | 2017-01-18 |
WO2014145176A1 (en) | 2014-09-18 |
CA2904863A1 (en) | 2014-09-18 |
EP2971134A1 (en) | 2016-01-20 |
JP2016512696A (ja) | 2016-05-09 |
EP2971134B1 (en) | 2023-10-25 |
CN105247076B (zh) | 2021-06-18 |
HK1219117A1 (zh) | 2017-03-24 |
JP2019176860A (ja) | 2019-10-17 |
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