CN105241853B - 一种全内反射荧光成像*** - Google Patents
一种全内反射荧光成像*** Download PDFInfo
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- CN105241853B CN105241853B CN201510562591.6A CN201510562591A CN105241853B CN 105241853 B CN105241853 B CN 105241853B CN 201510562591 A CN201510562591 A CN 201510562591A CN 105241853 B CN105241853 B CN 105241853B
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- dichroscope
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- 238000012632 fluorescent imaging Methods 0.000 title claims abstract description 25
- 238000003384 imaging method Methods 0.000 claims abstract description 45
- 230000003287 optical effect Effects 0.000 claims abstract description 41
- 238000002474 experimental method Methods 0.000 abstract description 5
- 230000035515 penetration Effects 0.000 abstract description 3
- 238000012163 sequencing technique Methods 0.000 description 12
- 238000005516 engineering process Methods 0.000 description 6
- 238000000034 method Methods 0.000 description 6
- 238000010586 diagram Methods 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 3
- 230000000295 complement effect Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 238000003752 polymerase chain reaction Methods 0.000 description 2
- 230000003321 amplification Effects 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 239000000571 coke Substances 0.000 description 1
- 238000007334 copolymerization reaction Methods 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000013401 experimental design Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000010287 polarization Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/648—Specially adapted constructive features of fluorimeters using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0052—Optical details of the image generation
- G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/18—Arrangements with more than one light path, e.g. for comparing two specimens
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/24—Base structure
- G02B21/241—Devices for focusing
- G02B21/245—Devices for focusing using auxiliary sources, detectors
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
- G02B27/10—Beam splitting or combining systems
- G02B27/14—Beam splitting or combining systems operating by reflection only
- G02B27/141—Beam splitting or combining systems operating by reflection only using dichroic mirrors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6417—Spectrofluorimetric devices
- G01N2021/6419—Excitation at two or more wavelengths
Landscapes
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Optics & Photonics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Microscoopes, Condenser (AREA)
Abstract
Description
Claims (7)
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510562591.6A CN105241853B (zh) | 2015-09-07 | 2015-09-07 | 一种全内反射荧光成像*** |
US15/752,417 US10481094B2 (en) | 2015-09-07 | 2016-09-06 | Total internal reflection fluorescence imaging system and sequencing device |
EP16843641.8A EP3348996B1 (en) | 2015-09-07 | 2016-09-06 | Total internal reflection fluorescence imaging system and sequencing device |
PCT/CN2016/098241 WO2017041703A1 (zh) | 2015-09-07 | 2016-09-06 | 全内反射荧光成像***及测序装置 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510562591.6A CN105241853B (zh) | 2015-09-07 | 2015-09-07 | 一种全内反射荧光成像*** |
Publications (2)
Publication Number | Publication Date |
---|---|
CN105241853A CN105241853A (zh) | 2016-01-13 |
CN105241853B true CN105241853B (zh) | 2019-05-07 |
Family
ID=55039575
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201510562591.6A Active CN105241853B (zh) | 2015-09-07 | 2015-09-07 | 一种全内反射荧光成像*** |
Country Status (4)
Country | Link |
---|---|
US (1) | US10481094B2 (zh) |
EP (1) | EP3348996B1 (zh) |
CN (1) | CN105241853B (zh) |
WO (1) | WO2017041703A1 (zh) |
Families Citing this family (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105241853B (zh) * | 2015-09-07 | 2019-05-07 | 深圳市瀚海基因生物科技有限公司 | 一种全内反射荧光成像*** |
CN105861293B (zh) * | 2016-04-06 | 2017-11-07 | 深圳市瀚海基因生物科技有限公司 | 单分子基因测序仪 |
CN107703614A (zh) * | 2016-08-08 | 2018-02-16 | 大连光耀辉科技有限公司 | 激光输出设备和荧光显微镜 |
CN106772975B (zh) * | 2017-01-19 | 2019-08-13 | 宁波纳美致生物科技有限公司 | 针对细胞或组织不同深度超分辨率显微成像的照明*** |
WO2019198553A1 (en) * | 2018-04-11 | 2019-10-17 | Sony Corporation | Microscope system and medical light source apparatus |
JP2019185002A (ja) | 2018-04-11 | 2019-10-24 | ソニー株式会社 | 顕微鏡システム及び医療用光源装置 |
CN111308726A (zh) * | 2018-12-12 | 2020-06-19 | 深圳市真迈生物科技有限公司 | 光学***、调校光学***的方法和测序*** |
CN109765213B (zh) * | 2019-03-27 | 2024-03-29 | 苏州威邦震电光电技术有限公司 | 相干反斯托克斯拉曼散射显微镜成像装置 |
CN110927948A (zh) * | 2019-12-05 | 2020-03-27 | 锘海生物科学仪器(上海)股份有限公司 | 具有固态光学***的荧光显微镜 |
CN113155826A (zh) * | 2020-01-07 | 2021-07-23 | 深圳华大智造科技有限公司 | 检测装置 |
US11320380B2 (en) * | 2020-04-21 | 2022-05-03 | Sartorius Bioanalytical Instruments, Inc. | Optical module with three or more color fluorescent light sources and methods for use thereof |
US11786116B2 (en) * | 2020-10-21 | 2023-10-17 | Excelitas Canada, Inc. | Multiple source endoscopy illumination system with adjustable angular distribution and wide field of view |
CN113758417B (zh) * | 2021-08-19 | 2024-02-23 | 大连工业大学 | 内窥式深孔内表面倍增成像装置 |
CN113866139A (zh) * | 2021-09-06 | 2021-12-31 | 北京谊安和景生物科技有限公司 | 一种高通量核酸检测光学*** |
CN114134025B (zh) * | 2021-11-19 | 2023-08-22 | 中国科学院长春光学精密机械与物理研究所 | 基因测序***及其测序方法 |
CN115820404A (zh) * | 2023-02-23 | 2023-03-21 | 深圳赛陆医疗科技有限公司 | 光学***、基因测序仪和基因测序*** |
CN116879240B (zh) * | 2023-09-07 | 2023-12-05 | 深圳市启扬光学科技有限公司 | 一种光学晶体的检测*** |
Citations (4)
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CN102405431A (zh) * | 2009-03-11 | 2012-04-04 | 美国樱花检验仪器株式会社 | 自动聚焦方法和自动聚焦设备 |
CN202975465U (zh) * | 2012-12-14 | 2013-06-05 | 王逸斐 | 一种光学显微镜自动聚焦检测装置 |
CN104316506A (zh) * | 2014-10-14 | 2015-01-28 | 上海交通大学 | 拉曼探头和可自动对焦的拉曼信号探测***以及方法 |
CN104568877A (zh) * | 2014-12-25 | 2015-04-29 | 中国科学院苏州生物医学工程技术研究所 | 基于led光源的随机光学重建显微成像***及方法 |
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JPH0769162B2 (ja) | 1990-04-23 | 1995-07-26 | 大日本スクリーン製造株式会社 | 光学的検査システムのための自動焦点合わせ装置 |
FI104097B1 (fi) | 1998-04-28 | 1999-11-15 | Valmet Corp | Paperikoneen puristinosa ja menetelmä sen puristuskudosten vaihdossa |
US6433929B1 (en) | 2000-06-12 | 2002-08-13 | Olympus Optical Co., Ltd. | Scanning optical microscope and method of acquiring image |
US6750457B2 (en) | 2001-08-29 | 2004-06-15 | Becton Dickinson And Company | System for high throughput analysis |
JP4792207B2 (ja) | 2004-06-09 | 2011-10-12 | オリンパス株式会社 | 走査型レーザ観察装置、観察方法及び観察プログラム |
US8443171B2 (en) * | 2004-07-30 | 2013-05-14 | Hewlett-Packard Development Company, L.P. | Run-time updating of prediction hint instructions |
WO2006055521A2 (en) * | 2004-11-16 | 2006-05-26 | Helicos Biosciences Corporation | Tirf single molecule analysis and method of sequencing nucleic acids |
WO2009042862A1 (en) * | 2007-09-28 | 2009-04-02 | Illumina, Inc | Fluorescence excitation and detection system and method |
JP2009198903A (ja) * | 2008-02-22 | 2009-09-03 | Olympus Corp | 光学機器 |
JP5386940B2 (ja) * | 2008-11-10 | 2014-01-15 | 株式会社ニコン | 顕微鏡 |
US20110294116A1 (en) * | 2008-12-11 | 2011-12-01 | The Regents of the Unicersity of California | Methods and systems for direct sequencing of single dna molecules |
JP2012003198A (ja) * | 2010-06-21 | 2012-01-05 | Olympus Corp | 顕微鏡 |
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CN104293648B (zh) * | 2014-09-29 | 2016-08-24 | 大族激光科技产业集团股份有限公司 | 基因测序光路*** |
CN105241853B (zh) * | 2015-09-07 | 2019-05-07 | 深圳市瀚海基因生物科技有限公司 | 一种全内反射荧光成像*** |
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2015
- 2015-09-07 CN CN201510562591.6A patent/CN105241853B/zh active Active
-
2016
- 2016-09-06 WO PCT/CN2016/098241 patent/WO2017041703A1/zh active Application Filing
- 2016-09-06 US US15/752,417 patent/US10481094B2/en active Active
- 2016-09-06 EP EP16843641.8A patent/EP3348996B1/en active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
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CN102405431A (zh) * | 2009-03-11 | 2012-04-04 | 美国樱花检验仪器株式会社 | 自动聚焦方法和自动聚焦设备 |
CN202975465U (zh) * | 2012-12-14 | 2013-06-05 | 王逸斐 | 一种光学显微镜自动聚焦检测装置 |
CN104316506A (zh) * | 2014-10-14 | 2015-01-28 | 上海交通大学 | 拉曼探头和可自动对焦的拉曼信号探测***以及方法 |
CN104568877A (zh) * | 2014-12-25 | 2015-04-29 | 中国科学院苏州生物医学工程技术研究所 | 基于led光源的随机光学重建显微成像***及方法 |
Also Published As
Publication number | Publication date |
---|---|
EP3348996A4 (en) | 2019-04-17 |
US20190011365A1 (en) | 2019-01-10 |
EP3348996A1 (en) | 2018-07-18 |
US10481094B2 (en) | 2019-11-19 |
EP3348996B1 (en) | 2020-06-24 |
CN105241853A (zh) | 2016-01-13 |
WO2017041703A1 (zh) | 2017-03-16 |
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Address after: 518000 Shenye Jinyuan Building, No. 116 Qingshuihe Road, Qingshuihe Street, Luohu District, Shenzhen City, Guangdong Province, 2 5th and 6th floors Patentee after: Shenzhen Zhenmai Biotechnology Co.,Ltd. Address before: 518000 National Supercomputing Shenzhen Center Research Building, No. 9 Tuxue Road, Xili University Town, Nanshan District, Shenzhen City, Guangdong Province, 10th Floor Patentee before: DIRECT GENOMICS Co.,Ltd. |
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Address after: 518000 podium 502A and 502B, podium 602, Luohu investment holding building, No. 112, Qingshuihe 1st Road, Qingshuihe community, Qingshuihe street, Luohu District, Shenzhen, Guangdong Patentee after: Shenzhen Zhenmai Biotechnology Co.,Ltd. Country or region after: China Address before: 518000 5th and 6th floors, block 2, Shenye Jinyuan Building, No.116, Qingshuihe 1st Road, Qingshuihe street, Luohu District, Shenzhen City, Guangdong Province Patentee before: Shenzhen Zhenmai Biotechnology Co.,Ltd. Country or region before: China |