CN105219811B - Enzymatic fatty acid mixed synthesizes human milk fat substituted product and preparation method thereof in a kind of microwave - Google Patents
Enzymatic fatty acid mixed synthesizes human milk fat substituted product and preparation method thereof in a kind of microwave Download PDFInfo
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Abstract
Enzymatic fatty acid mixed synthesizes human milk fat substituted product and preparation method thereof in a kind of microwave, is respectively placed in molecular sieve and saturated salt solution in closed container to control water activity；The free fatty acid olive oil purified by urea adduct method, coconut oil and algae oil are mixed, with sweet three ester of palmitinic acid together as the substrate of lipase enzymic transesterification, mixing is placed in same reaction flask, and reaction flask and the filter paper for filling lipase are respectively put into each closed container and are balanced at room temperature；After upper step balance, take out substrate, that lipase is put into screw socket vial is closed, enzyme process ester exchange reaction is carried out in constant temperature waters shaking table and microwave reactor respectively, the obtained grease of reaction is isolated through vacuum distillation and refines to get human milk fat substituted object.Palmitic acid content in reacting final product of the present invention rich in DHA and on its position sn-2 is also relatively high, it is ensured that with palmitic acid content is suitable on the position sn-2 in natural human milk fat.
Description
Technical field
The present invention relates to biocatalysis fields, and in particular to a kind of free fatty acid of lipase-catalyzed miscella and palm
Sweet three ester (tripalmitin, PPP) of acid synthesizes human milk fat substituted product and preparation method thereof.
Background technique
Human milk fat is as the important nutrient during infant growth, its building to baby cells film, dimension
Absorption, energy supplement and the intellectual development of raw element have important role.It is needed after breast milk is insufficient or baby's wean
When increasing diatery supplement, human milk fat substituted product (Human milk fat subsititute, HMFS) are as the baby for replacing breast milk
Food is fed, structure function should be with natural human milk fat and its similar, the nutriment that can be provided in addition to providing breast milk
Except, it is necessary to give infant more and facilitate them body, the essential elements and organic matter of intellectual development.Add HMFS's
Formula milk is mainly absorbed in the form of sn-2 palmitic acid monoglyceride and free oleic acid in vivo, prevents free palm
Acid and Ga2+Forming insoluble soap calcium influences fatty acid and Ga2+Absorption, cause a large amount of calcareous loss (Lipid
Technology, 2010,22 (6): 126-129), so as to cause constipation of infantile and abdominal pain.
In addition, compared with the baby of feeding ordinary powdered milk, baby's bone of feeding high-content sn-2 palmitinic acid formula milk
The absorptivity of minerals is higher, to preferably support the natural growth of infant physique and bone.Therefore, the chemistry knot of HMFS
Structure and fatty acid composition are an important factor for influencing baby formula milk powder trophic function.
Currently, the raw material of the artificial synthesized HMFS on current market mainly has the animal oil such as fish oil, lard, butter fat,
The vegetable oil such as palm oil, rapeseed oil, although abundance, these substrates have the shortcomings that corresponding.As fish oil is oxidizable not
Stablize, excessive stearic acid is contained in lard, easily product is made to agglomerate, influences baby and absorb and can be limited by humane religion factor；
Cow's milk replaces breast milk directly to feed, it is difficult to be absorbed by baby, be eliminated by market；Although its composition and HMFS in vegetable oil
With similitude, but in fatty acid triglycercide distributed architecture difference (American Dairy Science
Association, 2007,90 (4): 2147-2154), it results in baby and is still difficult to absorb.To sum up, these raw material sources
All various limitations are suffered from, and nutritive value is also very different；Preparation process is cumbersome, the easy oxygen of unsaturated fatty acid
Change, high production cost；The limitation objective reality of the regional cultures such as country variant, area, nationality and culture background.Therefore, not
It is the optimum feed stock of artificial synthesized HMFS.
In recent years, with the exhaustion of land resources, promote the mankind to move towards ocean and seek novel resource, at the same time, easily
Culture, high grease, the appearance of microalgae rich in DHA, fatty acid composition and varying distribution bring mankind's unprecedented opportunities.
Studies have shown that algae oil is as a kind of emerging pure natural grease, total content of polyunsaturated fatty acid is very high (50% or more), contains
There is the omega-3 unsaturated fatty acids such as DHA, DPA abundant (European Journal of Lipid Science and
Technology, 2013,115 (9): 965-976), and have many advantages, such as that safe and pollution-free, nutritive value is high, theoretically
Prepare the quality raw materials of functionality HMFS.But the own characteristic due to fatty acid composition in algae oil triglycerides and being distributed,
Sn-1,3 contain a certain amount of palmitinic acid, are unfavorable for the digestion and absorption of infant, cannot be directly used as HFMS.Therefore, this is special
Natural algae oil is carried out reasonable enzyme process structure of modification by benefit selection, by its with rich in short carbon chain coconut oil, rich in single insatiable hunger
It is prepared into acry radical donor according to corresponding ratio with the olive oil of fatty acid and the algae oil rich in DHA, more with PPP enzyme' s catalysis
The breast milk HMFS for meeting infant nutrient demand is a kind of very reasonable and practicable approach.
Enzyme process prepares human milk fat substituted product, and there are also biocatalysis other than the R and D of above-mentioned enzyme and raw material
The improvement of technology.Since grease viscosity is higher, negative impact is brought to the mass-and heat-transfer effect of reaction process, however, in recent years
The mass transfer for enhancing liquid-liquid heterogeneous system using low-temperature microwave technology to develop is a kind of effective solution grease mobility
The means (Bioresource Technology, 2011,102 (11): 6617-6620) of problem.It is micro- compared with traditional reactor
Wave field strengthens the field-effect that can enhance esters synthetic reaction, promotes enzymatic reaction efficiently to carry out, substantially increases target product
Space-time yield, shorten the reaction time, improve enzyme stability (Bioresource Technology, 2013,149:367-
374) the advantages that, is widely used in biocatalysis, especially in biocatalysis in non-conventional media.
" microwave radiation-enzyme coupling and catalyzing technology " has been widely used in experimental study (Bioresource at present
Technology, 2010,101 (13): 4851-4861), constant reaction temperature is combined with constant microwave irradiation power, is made
The nonthermal effect of microwave is significantly strengthened.However, so far, synthesizing human milk fat substituted product using microwave-assisted enzymatic
Document report it is less, still, it is contemplated that the field of microwave causes characteristic, its application in HMFS synthesis will be gradually expanded.Cause
This, investigated herein lipase-catalyzed mixing free fatty acid reacted with PPP preparation HMFS while, by control water activity come
Attempt to improve efficiency of pcr product, low-temperature microwave technical application is illustrated micro- to the transesterification preparation of enzymatic rich on the HMFS of DHA respectively
The influence transesterification to enzymatic of water, microwave.
Summary of the invention
The technical issues of solution: the present invention is directed to the correlation of existing HMFS for infant formula and preparation method thereof
Problem with the olive oil of rich oil palmitinic acid (C16:0), contains the short carbon chains fatty acid such as sad (C8:0), lauric acid (C10:0)
Coconut oil, and the microalgae rich in DHA are raw material, provide enzymatic fatty acid mixed in a kind of microwave synthesize human milk fat substituted product and
Preparation method, sn-1 in selective enrichment triglycerides, the content of the unsaturated fatty acids such as 3 upper DHA, the HMFS prepared
Without additionally adding DHA again.A process for preparing HMFS improve the content of palmitinic acid on the position sn-2, can and human milk fat
Sn-2 are consistent, i.e., and 60% or more.Have very great help for the function raising of HMFS.
Technical solution: enzymatic fatty acid mixed synthesizes the preparation method of human milk fat substituted product, step in a kind of microwave
Are as follows: 1) control method of water activity: respectively willMolecular sieve and LiBr, LiCl, CH3COOK、Mg(NO3)2·6H2O、NaCl、
KCl、K2Cr2O7It is configured to saturated solution to be placed in closed container, at room temperatureMolecular sieve is for controlling water activity <
0.01, the water activity that each saturated solution is controlled is respectively 0.05,0.11,0.23,0.54,0.75,0.85,0.95；It will lead to
It is 70:15:15~98 that free fatty acid olive oil, coconut oil and algae oil that urea adduct method purifies, which are crossed, according to mass ratio:
1:1 mixing, with sweet three ester of palmitinic acid together as the substrate of lipase enzymic transesterification, and by free fatty acid mixture and palm fibre
The sweet three esters mixing of palmitic acid acid is placed in same reaction flask, and reaction flask and the filter paper for filling lipase are respectively put into each closed appearance
2-3d is balanced in device at room temperature；2) lipase-catalyzed acidolysis: after upper step balance, substrate is taken out, lipase is put into screw socket
Vial is closed, carries out enzyme process ester exchange reaction, the palmitinic acid sweet three in constant temperature waters shaking table and microwave reactor respectively
Ester is 1:1~1:15 with the mixing mass ratio for mixing free fatty acid, after ester exchange reaction, isolates reaction institute through vacuum distillation
Obtained grease simultaneously refines to get human milk fat substituted object.
The lipase be Lipozyme RM IM, Lipozyme IM60, Lipozyme IM20, Lipase SP435,
Lipase SP382、Candida rugosa lipase、Lipase MC7、Lipozyme TL IM、Novozym 435、
Candida antarctica lipase B, R275A lipase or porcine pancreatic lipase.
The solvent for being configured to saturated solution is ethyl alcohol, n-butanol, n-hexane, hexamethylene, ethyl acetate or acetic acid fourth
Ester.
The mass ratio that the lipase accounts for reaction system is 1%~15%, the reaction temperature of the enzyme process ester exchange reaction
Degree is 30~80 DEG C, and the reaction time is 15~120min.
The human milk fat substituted product that the above method is prepared.
The utility model has the advantages that the present invention is using sn-1,3 specific lipase catalytic mixing fatty acid and PPP controlled syntheses
HMFS.It is also relatively high rich in the palmitic acid content on DHA and its position sn-2 in reacting final product, it is ensured that with natural breast milk
Palmitic acid content is suitable on the position sn-2 in fat, that is, reaches 70% or so.Importantly, by low-temperature microwave technical application to enzyme
Promote transesterification to be combined in HMFS, provide fundamental basis for prepare with scale HMFS from now on and technical support, foreign countries' monopolization is produced
There is positive reality to refer to for the production domesticization of product, the resource utilization for improving oil-rich microalgae, the industrial chain for extending China's dairy industry
Lead meaning.
Specific embodiment
Present invention will be further explained below with reference to specific examples.It should be understood that these embodiments are merely to illustrate the present invention
Rather than it limits the scope of the invention.In addition, it should also be understood that, after reading the content taught by the present invention, those skilled in the art
Member can make various changes or modifications the present invention, and such equivalent forms equally fall within the application the appended claims and limited
Range.
The method of qualitative and quantitative analysis for fatty acid is high resolution gas chromatography, condition are as follows: uses Agilent
6820 gas chromatographs, model HP-INNOWAX, column length 30m, outer diameter 0.25mm, 0.25 μm of internal diameter, using gradient increased temperature
Mode, 80 DEG C of initial temperature, 250 DEG C of post case maximum temperature, 250 DEG C of rear injection port maximum temperature, rear detector maximum temperature 280
DEG C, 43min is amounted to, sample volume is 1 μ L.
Embodiment 1
This example demonstrates that the fatty acid compositional analysis of substrate used in transesterification.
It purifies olive oil, coconut oil, algae oil to obtain free fatty acid by urea adduct method respectively, by three kinds of oily trips
It is surveyed after 70:15:15~98:1:1 (olive oil: coconut oil: algae oil) mixing by gas-chromatography from fatty acid according to mass ratio
It is fixed, obtain in olive oil, coconut oil and algae oil and mixing free fatty acid (with quality than olive oil: coconut oil: algae oil=
For 90:5:5) content of each fatty acid see the table below 1.Free fatty acid is mixed used in subsequent embodiment is with the present embodiment
Raw material.
Each rouge in 1 olive oil of table, coconut oil and algae oil and miscella (olive oil: coconut oil: algae oil=90:5:5)
The content of fat acid
A: it is not detected
Following embodiment illustrates the process for making catalyst esterification with lipase.
Embodiment 2
By PPP (0.62g) with mix free fatty acid (1.88g) molar ratio 1:9 mix after, be added sn-1,3 specificity
Lipozyme RM IM 10% (0.25g) is put into 60 DEG C of shaking baths and reacts 3h.
Take out 500 μ L after reaction, 60mg porcine pancreatic lipase be added and hydrolyzes 3min, is extracted with ether, take organic layer into
Row thin-layer chromatography scrapes sn-2 monoglyceride bands.2mL n-hexane and 2mL 0.5mol KOH- methanol solution is added at 65 DEG C
60min is reacted in shaking bath and carries out esterification, is then carried out gas chromatographic detection, is measured each content of fatty acid in product grease
Such as the following table 2.
Embodiment 3
After PPP (0.74g) is mixed with mixed free fatty acid (1.76g) molar ratio 1:7, sn-1 is added, 3 special
Property Lipozyme RM IM 6% (0.15g), is put into 60 DEG C of shaking baths and reacts 3h.
Take out 500 μ L after reaction, 60mg porcine pancreatic lipase be added and hydrolyzes 3min, is extracted with ether, take organic layer into
Row thin-layer chromatography scrapes sn-2 monoglyceride bands.2mL n-hexane and 2mL 0.5mol KOH- methanol solution is added at 65 DEG C
60min is reacted in shaking bath and carries out esterification, is then carried out gas chromatographic detection, is measured each content of fatty acid in product grease
Such as the following table 3.
Embodiment 4
After PPP (0.74g) is mixed with mixed free fatty acid (1.76g) molar ratio 1:7, sn-1 is added, 3 special
Property Lipozyme RM IM 10% (0.25g), is put into 70 DEG C of shaking baths and reacts 3h.
Take out 500 μ L after reaction, 60mg porcine pancreatic lipase be added and hydrolyzes 3min, is extracted with ether, take organic layer into
Row thin-layer chromatography scrapes sn-2 monoglyceride bands.2mL n-hexane and 2mL 0.5mol KOH- methanol solution is added at 65 DEG C
60min is reacted in shaking bath and carries out esterification, is then carried out gas chromatographic detection, is measured each content of fatty acid in product grease
Such as the following table 4.
Embodiment 5
After PPP (0.93g) is mixed with mixed free fatty acid (1.57g) molar ratio 1:5, sn-1 is added, 3 special
Property Lipozyme RM IM 10% (0.25g), reaction temperature be 60 DEG C, microwave reinforced power be 100W, the time
60min。
Take out 500 μ L after reaction, 60mg porcine pancreatic lipase be added and hydrolyzes 3min, is extracted with ether, take organic layer into
Row thin-layer chromatography scrapes sn-2 monoglyceride bands.2mL n-hexane and 2mL 0.5mol KOH- methanol solution is added at 65 DEG C
60min is reacted in shaking bath and carries out esterification, is then carried out gas chromatographic detection, is measured each content of fatty acid in product grease
Such as the following table 5.
A: it is not detected
Embodiment 6
After PPP (0.62g) is mixed with mixed free fatty acid (1.88g) molar ratio 1:9, sn-1 is added, 3 special
Property Lipozyme RM IM 8% (0.20g), reaction temperature be 50 DEG C, microwave reinforced power be 100W, the time
60min。
Take out 500 μ L after reaction, 60mg porcine pancreatic lipase be added and hydrolyzes 3min, is extracted with ether, take organic layer into
Row thin-layer chromatography scrapes sn-2 monoglyceride bands.2mL n-hexane and 2mL 0.5mol KOH- methanol solution is added at 65 DEG C
60min is reacted in shaking bath and carries out esterification, is then carried out gas chromatographic detection, is measured each content of fatty acid in product grease
Such as the following table 6.
A: it is not detected
Embodiment 7
After PPP (0.74g) is mixed with mixed free fatty acid (1.76g) molar ratio 1:7, sn-1 is added, 3 special
Property Lipozyme RM IM 10% (0.25g), reaction temperature be 50 DEG C, microwave reinforced power be 100W, the time
30min。
Take out 500 μ L after reaction, 60mg porcine pancreatic lipase be added and hydrolyzes 3min, is extracted with ether, take organic layer into
Row thin-layer chromatography scrapes sn-2 monoglyceride bands.2mL n-hexane and 2mL 0.5mol KOH- methanol solution is added at 65 DEG C
60min is reacted in shaking bath and carries out esterification, is then carried out gas chromatographic detection, is measured each content of fatty acid in product grease
Such as the following table 7.
A: it is not detected
Embodiment 8
After PPP (0.74g) is mixed with mixed free fatty acid (1.76g) molar ratio 1:7, sn-1 is added, 3 special
Property Lipozyme RM IM 10% (0.25g), reaction temperature be 30 DEG C, microwave reinforced power be 100W, the time
60min。
Take out 500 μ L after reaction, 60mg porcine pancreatic lipase be added and hydrolyzes 3min, is extracted with ether, take organic layer into
Row thin-layer chromatography scrapes sn-2 monoglyceride bands.2mL n-hexane and 2mL 0.5mol KOH- methanol solution is added at 65 DEG C
60min is reacted in shaking bath and carries out esterification, is then carried out gas chromatographic detection, is measured each content of fatty acid in product grease
Such as the following table 8.
A: it is not detected
Embodiment 9
After PPP (0.74g) is mixed with mixed free fatty acid (1.76g) molar ratio 1:7, with sn-1,3 specificity
Lipozyme RM IM 10% (0.25g), is individually placed to fillThe closed glass container of molecular sieve (aw < 0.01)
It is interior, it takes out and mixes after balancing 3 days at room temperature.Reaction temperature is 50 DEG C, and microwave reinforced power is 100W, time 60min.
Take out 500 μ L after reaction, 60mg porcine pancreatic lipase be added and hydrolyzes 3min, is extracted with ether, take organic layer into
Row thin-layer chromatography scrapes sn-2 monoglyceride bands.2mL n-hexane and 2mL 0.5mol KOH- methanol solution is added at 65 DEG C
60min is reacted in shaking bath and carries out esterification, is then carried out gas chromatographic detection, is measured each content of fatty acid in product grease
Such as the following table 9
A: it is not detected
Embodiment 10
After PPP (0.74g) is mixed with mixed free fatty acid (1.76g) molar ratio 1:7, with sn-1,3 specificity
Lipozyme RM IM 10% (0.25g) is individually placed to fill the closed glass of saturation NaCl solution (aw=0.75)
In container, takes out and mix after balancing 3 days at room temperature.Reaction temperature is 50 DEG C, and microwave reinforced power is 100W, time 60min.
Take out 500 μ L after reaction, 60mg porcine pancreatic lipase be added and hydrolyzes 3min, is extracted with ether, take organic layer into
Row thin-layer chromatography scrapes sn-2 monoglyceride bands.2mL n-hexane and 2mL 0.5mol KOH- methanol solution is added at 65 DEG C
60min is reacted in shaking bath and carries out esterification, is then carried out gas chromatographic detection, is measured each content of fatty acid in product grease
Such as the following table 10.
A: it is not detected
Embodiment 11
Total fatty acids and the comparative situation of sn-2 content of fatty acid and human milk fat in the HMFS of this method controlled syntheses
As shown in table 11.Compared with human milk fat, in reacting final product made from this method oleic acid Percentage bound be up to 54.40 ±
1.76%, it is rich in short carbon chain fatty acid, and its sn-2 palmitic acid content is relatively high, the digestion that may advantageously facilitate infant is inhaled
It receives.
Total fatty acids and sn-2 fatty acid and human milk fat compare in the HMFS of 11. this method of table preparation
A: it is not detected；b:Journal of Agriculturaland Food Chemistry.2012,61(1):
167-175.
Note: the specific data corresponding embodiment 10 of HMFS described in table 11.
Claims (1)
1. enzymatic fatty acid mixed synthesizes the preparation method of human milk fat substituted product in a kind of microwave, it is characterised in that its step
Are as follows: 1) control method of water activity: respectively willMolecular sieve and NaCl are configured to saturated solution and are placed in closed container,
At room temperatureMolecular sieve is 0.75 for controlling water activity < 0.01, the water activity that saturated solution is controlled；Urea packet will be passed through
Free fatty acid olive oil, coconut oil and the algae oil that legal purifying obtains are 90:5:5 mixing according to mass ratio；2) lipase is urged
Change acidolysis: after the free fatty acid that sweet three ester of 0.74g palmitinic acid is mixed with 1.76g mixes, with 0.25g sn-1,3 special
Property Lipozyme RM IM, in the closed glass container for being individually placed to fill saturation NaCl solution, balance 3 days at room temperature
After take out and mix, reaction temperature is 50 DEG C, and microwave reinforced power is 100W, time 60min, isolates reaction through vacuum distillation
Obtained grease simultaneously refines to get human milk fat substituted object.
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| CN103667379A (en) * | 2013-12-17 | 2014-03-26 | 江苏科技大学 | Method for preparing breast milk fat substitute through lipase-catalyzed acidolysis of algae oil |
| CN103689101A (en) * | 2013-12-17 | 2014-04-02 | 江苏科技大学 | Preparation method of synthetic human milk fat substitute (HMFS) of chrysalis oil source |
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| CN103667379A (en) * | 2013-12-17 | 2014-03-26 | 江苏科技大学 | Method for preparing breast milk fat substitute through lipase-catalyzed acidolysis of algae oil |
| CN103689101A (en) * | 2013-12-17 | 2014-04-02 | 江苏科技大学 | Preparation method of synthetic human milk fat substitute (HMFS) of chrysalis oil source |
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Application publication date: 20160106 Assignee: Jiangsu runmeng Ecological Agriculture Development Co., Ltd Assignor: JIANGSU University OF SCIENCE AND TECHNOLOGY Contract record no.: X2020980007223 Denomination of invention: Synthesis of breast milk fat substitute by enzyme catalyzed mixed fatty acids in microwave and its preparation method Granted publication date: 20190118 License type: Common License Record date: 20201029 |
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