CN105213442A - A kind of Fructus Nitraria schoberi extractive of general flavone and preparation method thereof - Google Patents
A kind of Fructus Nitraria schoberi extractive of general flavone and preparation method thereof Download PDFInfo
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Abstract
The present invention relates to a kind of Fructus Nitraria schoberi extractive of general flavone and preparation method thereof, the Fructus Nitraria schoberi obtained by preparation methoies such as cleaning, drying, fragmentation, remove impurity, extraction, purification has slow down aging, blood sugar lowering, antilipemic healthy function, Fructus Nitraria schoberi extractive of general flavone preparation method technique of the present invention is simple simultaneously, be easy to operation, yield is higher, quality is good, is conducive to realizing industrialized production and processing.
Description
Technical field
The present invention relates to plant extract field, be specially a kind of extract and preparation method thereof with slow down aging, blood sugar lowering, antilipemic healthy function Fructus Nitraria schoberi.
Background technology
It is fat that Fructus Nitraria schoberi (NitrariatangutoumBor) has another name called acid, there is the title of " Fructus seu semen pruni szechuanicae ", that Herba pteridii latiusculi Chenopodiaceae (Zygophyllceae) Fructus Nitraria schoberi belongs to (Nitearia) machaka, Berry-like drupe, 7 ~ August is ripe, is mainly distributed in the ground such as the Inner Mongol, Ningxia, Gansu, Xinjiang, Qinghai.Cortex Acanthopanacis Radicis has higher medical value, is used as medicine with a long history in northwest minority areas.Cortex Acanthopanacis Radicis, Ancient Chinese is called Dong Se, husky fluffy rice, popular name western sand cherry.Supplement to the Herbal: “ Dong Se, sweet in the mouth is flat, nontoxic, and QI invigorating is made light of one's life by commiting suicide, and clothes are not hungry for a long time, hard muscles and bones ".Describe in Compendium of Material Medica, " abnormal smells from the patient is pungent, cold, nontoxic for Cortex Acanthopanacis Radicis.Cure mainly angina pectoris, the swollen pus of bursting of pain, pain relieving.Treat husband deficient, sexual impotence essence goes out certainly, the kidney invigorating gas, beneficial marrow etc. ".Cortex Acanthopanacis Radicis is the endemic species of China, containing abundant nutritional labeling and active substance in its fruit, can be used as medicine, have regulating menstruation and activating blood, the effects such as digestion-promoting spleen-invigorating.Cortex Acanthopanacis Radicis is called Herba Carlinae biebersteinii in " Chinese medicine voluminous dictionary ", gathers, dry and be used as medicine time ripe with fruit.There is the function of strengthening the spleen and stomach, strengthening by means of tonics, regulating menstruation and activating blood, cure mainly asthenic body, deficiency of both QI and blood, incoordination between the spleen and stomach, dyspepsia, menoxenia, deficiency and coldness lumbago.For medicine is commonly used by the Uygur nationality, claim Acker sheep Plutarch crow to draw lid, there is the effects such as strengthening the spleen and stomach, blood pressure lowering, regulating menstruation and activating blood, lactogenic.Local ethnic groups disease such as Fructus Nitraria schoberi treatment dyspepsia and hypertension dizziness etc., evident in efficacy.Modern medicine study is thought, the sweet acid of Cortex Acanthopanacis Radicis nature and flavor, the diseases such as micro-salty temperature, cures mainly physical weakness, deficiency of both QI and blood, menoxenia, waist abdomen pain.There is Adjust-blood lipid, blood pressure lowering, blood sugar lowering and antioxidation.
Containing various active composition in berry of nitraria tangutorum bobr.Except aminoacid, flavone is a topmost active component in Cortex Acanthopanacis Radicis, is the basis that Fructus Nitraria schoberi possesses the effects such as Adjust-blood lipid antioxidation, blood pressure lowering and blood sugar lowering.
The general fashion that currently available technology prepares Fructus Nitraria schoberi extractive of general flavone is simple alcohol reflux, and the not high flavone yield that causes of the extraction efficiency of this extracting method is lower, and organic solvent reclaims not exclusively, and extraction cost is higher etc.
Summary of the invention
The object of this invention is to provide the extracting method of the Fructus Nitraria schoberi total flavones that a kind of loss of effective components is few, quality is high, extraction cost is low.The Fructus Nitraria schoberi total flavones that the method extracts possesses the effects such as Adjust-blood lipid, antioxidation, blood pressure lowering, slow down aging and blood sugar lowering.
Concrete, extracting method of the present invention is as follows:
An extracting method for Fructus Nitraria schoberi total flavones, is characterized in that, adopts Fructus Nitraria schoberi, after fragmentation, adopts 80% alcohol reflux.
An extracting method for Fructus Nitraria schoberi total flavones, is characterized in that comprising the following steps:
1, clear up: select ripe Fructus Nitraria schoberi, screen out silt, clean with clear water;
2, dry: by the Fructus Nitraria schoberi cold drying 10 ~ 24h at 5 ~ 30 DEG C cleaned up;
3, broken: Fructus Nitraria schoberi step 2 obtained carries out micronizing, and obtaining particle size range is 1 ~ 75 μm of powder;
4, extract: 50% ethanol ~ 80% ethanol adding 5 ~ 8 times amount in Fructus Nitraria schoberi powder step 3 obtained carries out supersound extraction 0.5h ~ 1.5h, period stirs with agitator, filtrate I and filtering residue I is obtained after filtration, 50% ethanol ~ 80% ethanol filtering residue I being reused 5 ~ 8 times amount carries out reflux, extract, 0.5h ~ 1.5h, filtrate II and filtering residue II is obtained after filtration, filtrate I and filtrate II are merged, after concentrating under reduced pressure, obtains the total flavones crude extract part of Fructus Nitraria schoberi;
5, purification: will through pretreated polyamide wet method dress post, get above-mentioned crude extract in the different in flow rate loading of 0.2 ~ 0.8ml/min, with the ethanol of 70% concentration with the different in flow rate eluting of 0.5 ~ 1.5ml/min, eluent is merged, concentrated, be drying to obtain the flavone extract that purity is higher.
This extracting method be through a large amount of experiment of inventor determine and screen obtain, can preparation process be reduced, and the Fructus Nitraria schoberi extractive of general flavone that loss of effective components is few, quality is high, cost is low can be obtained.
In the first step, if carefully do not clean, the silt dead leaf etc. of doping can affect extraction efficiency, even causes mechanical breakdown and accident.
In second step, under low temperature, carry out drying, the loss of flavone effective ingredient can be ensured.
In 3rd step, superfine communication technique can make powder diameter little (generally below 15 μm) and be evenly distributed, ball degree and homogeneity obviously improve, bulk density and specific surface significantly improve, plant cell wall breaking rate is high, dissolving and the release of the effective ingredient (particularly refractory components) in Fructus Nitraria schoberi are accelerated, and the dissolution rate of effective ingredient is accelerated.
In 4th step, have employed two kinds of extracting modes, first supersound extraction is carried out, hyperacoustic sound wave cavitation makes solid sample disperse, increase the contact area between sample and extractant, improve object transfers to liquid phase mass transfer speed from solid phase, period also carries out stirring with agitator and can reduce powder, adds the contact area of medical material and solvent.Next adopts reflux, extract, and extract solid-liquid ratio used by optimizing, extraction time, Extracting temperature and concentration of alcohol can increase substantially the general flavone content of Fructus Nitraria schoberi.Said extracted process only has two steps, simplifies the process of preparation, can not only substantially increase the efficiency of extraction step, and can reduce the loss of preparation process.
In 5th step, the separation and purification of polyamide column chromatography can obtain purer flavone extract.
In addition, for the consideration of controlling cost, the cold drying in each step of the present invention, backflow, ultrasonic, etc. are all ripe technology, simple to operate, easy left-hand seat.But compared with prior art, simplify leaching process and but obtain higher yield and the good product of quality.
Preferably, a kind of extracting method of Fructus Nitraria schoberi total flavones, is characterized in that comprising the following steps:
1, clear up: select ripe Fructus Nitraria schoberi, screen out silt, clean with clear water;
2, dry: by the Fructus Nitraria schoberi cold drying 15h at 15 DEG C cleaned up;
3, broken: Fructus Nitraria schoberi step 2 obtained carries out micronizing, obtains Fructus Nitraria schoberi powder;
4, extract: 50% ethanol adding 5 times amount in Fructus Nitraria schoberi powder step 3 obtained carries out supersound extraction 0.5h, period stirs with agitator, filtrate I and filtering residue I is obtained after filtration, 60% ethanol filtering residue I being reused 7 times amount carries out reflux, extract, 1h, filtrate II and filtering residue II is obtained after filtration, filtrate I and filtrate II are merged, after concentrating under reduced pressure, obtains the thick Extraction parts of total flavones of Fructus Nitraria schoberi;
5, purification: will through pretreated polyamide wet method dress post, get above-mentioned crude extract in the flow velocity loading of 0.3ml/min, with the ethanol of 70% concentration with the different in flow rate eluting of 1.5ml/min, eluent is merged, concentrated, be drying to obtain the flavone extract that purity is higher.
Preferably, a kind of extracting method of Fructus Nitraria schoberi total flavones, is characterized in that comprising the following steps:
1, clear up: select ripe Fructus Nitraria schoberi, screen out silt, clean with clear water;
2, dry: by the Fructus Nitraria schoberi cold drying 20h at 20 DEG C cleaned up;
3, broken: Fructus Nitraria schoberi step 2 obtained carries out micronizing, obtains Fructus Nitraria schoberi powder;
4, extract: 50% ethanol adding 8 times amount in Fructus Nitraria schoberi powder step 3 obtained carries out supersound extraction 0.5h, period stirs with agitator, filtrate I and filtering residue I is obtained after filtration, 60% ethanol filtering residue I being reused 7 times amount carries out reflux, extract, 1h, filtrate II and filtering residue II is obtained after filtration, filtrate I and filtrate II are merged, after concentrating under reduced pressure, obtains the thick Extraction parts of total flavones of Fructus Nitraria schoberi.
5, purification: will through pretreated polyamide wet method dress post, get above-mentioned crude extract in the flow velocity loading of 0.5ml/min, with the ethanol of 70% concentration with the different in flow rate eluting of 1.0ml/min, eluent is merged, concentrated, be drying to obtain the flavone extract that purity is higher.
Preferably, a kind of extracting method of Fructus Nitraria schoberi total flavones, is characterized in that comprising the following steps:
1, clear up: select ripe Fructus Nitraria schoberi, screen out silt, clean with clear water;
2, dry: by the Fructus Nitraria schoberi cold drying 15h at 15 DEG C cleaned up;
3, broken: micronizing to be carried out to Fructus Nitraria schoberi, obtains Fructus Nitraria schoberi powder;
4, extract: 50% ethanol adding 5 times amount in Fructus Nitraria schoberi powder is carried out supersound extraction 0.5h, period stirs with agitator, filtrate I and filtering residue I is obtained after filtration, 60% ethanol filtering residue I being reused 7 times amount carries out reflux, extract, 1h, filtrate II and filtering residue II is obtained after filtration, filtrate I and filtrate II are merged, after concentrating under reduced pressure, obtains the thick Extraction parts of total flavones of Fructus Nitraria schoberi;
5, purification: will through pretreated polyamide wet method dress post, get above-mentioned crude extract in the flow velocity loading of 0.8ml/min, with the ethanol of 70% concentration with the different in flow rate eluting of 1.ml/min, eluent is merged, concentrated, be drying to obtain the flavone extract that purity is higher.
Detailed description of the invention
Embodiment 1
1, clear up: select ripe Fructus Nitraria schoberi 10kg, screen out silt, clean with clear water;
2, dry: by the Fructus Nitraria schoberi cold drying 15h at 15 DEG C cleaned up;
3, broken: Fructus Nitraria schoberi step 2 obtained carries out micronizing, obtains Fructus Nitraria schoberi powder;
4, extract: 70% ethanol adding 5 times amount in Fructus Nitraria schoberi powder step 3 obtained carries out supersound extraction 1h, period stirs with agitator, filtrate I and filtering residue I is obtained after filtration, 70% ethanol filtering residue I being reused 7 times amount carries out reflux, extract, 1h, filtrate II and filtering residue II is obtained after filtration, filtrate I and filtrate II are merged, after concentrating under reduced pressure, obtains the thick Extraction parts of total flavones of Fructus Nitraria schoberi;
5, purification: will through pretreated polyamide wet method dress post, get above-mentioned crude extract in the flow velocity loading of 0.8ml/min, with the ethanol of 70% concentration with the different in flow rate eluting of 1.0ml/min, eluent is merged, concentrated, be drying to obtain the flavone extract that purity is higher.
After testing, the extraction ratio of the Fructus Nitraria schoberi total flavones that embodiment 1 is extracted is 1.977% far above 1.104% of prior art, and therefore the present invention has higher beneficial effect for the extraction of Fructus Nitraria schoberi total flavones.
Embodiment 2
1, clear up: select ripe Fructus Nitraria schoberi 10kg, screen out silt, clean with clear water;
2, dry: by the Fructus Nitraria schoberi cold drying 15h at 20 DEG C cleaned up;
3, broken: Fructus Nitraria schoberi step 2 obtained carries out micronizing, obtains Fructus Nitraria schoberi powder;
4, extract: 70% ethanol adding 8 times amount in Fructus Nitraria schoberi powder step 3 obtained carries out supersound extraction 1h, period stirs with agitator, filtrate I and filtering residue I is obtained after filtration, 80% ethanol filtering residue I being reused 6 times amount carries out reflux, extract, 1h, filtrate II and filtering residue II is obtained after filtration, filtrate I and filtrate II are merged, after concentrating under reduced pressure, obtains the thick Extraction parts of total flavones of Fructus Nitraria schoberi;
5, purification: will through pretreated polyamide wet method dress post, get above-mentioned crude extract in the flow velocity loading of 0.7ml/min, with the ethanol of 70% concentration with the different in flow rate eluting of 1.5ml/min, eluent is merged, concentrated, be drying to obtain the flavone extract that purity is higher.
After testing, the extraction ratio of the Fructus Nitraria schoberi total flavones that embodiment 2 is extracted is 1.87% far above 1.104% of prior art, and therefore the present invention has higher beneficial effect for the extraction of Fructus Nitraria schoberi total flavones.
Embodiment 3
1, clear up: select ripe Fructus Nitraria schoberi 10kg, screen out silt, clean with clear water;
2, dry: by the Fructus Nitraria schoberi cold drying 20h at 15 DEG C cleaned up;
3, broken: micronizing to be carried out to the Fructus Nitraria schoberi that step 2 obtains, obtains Fructus Nitraria schoberi powder;
4, extract: 70% ethanol adding 5 times amount in Fructus Nitraria schoberi powder step 3 obtained carries out supersound extraction 1.5h, period stirs with agitator, filtrate I and filtering residue I is obtained after filtration, 65% ethanol filtering residue I being reused 7 times amount carries out reflux, extract, 1.5h, filtrate II and filtering residue II is obtained after filtration, filtrate I and filtrate II are merged, after concentrating under reduced pressure, obtains the thick Extraction parts of total flavones of Fructus Nitraria schoberi;
5, purification: will through pretreated Buddhist nun's human relations polyamide wet method dress post, get above-mentioned crude extract in the flow velocity loading of 0.6ml/min, with the ethanol of 70% concentration with the different in flow rate eluting of 0.8ml/min, eluent is merged, concentrated, be drying to obtain the flavone extract that purity is higher.
After testing, the extraction ratio of the Fructus Nitraria schoberi total flavones that embodiment 3 is extracted is 1.88% far above 1.104% of prior art, and therefore the present invention has higher beneficial effect for the extraction of Fructus Nitraria schoberi total flavones.
Embodiment 4
The extraction effect contrast of the total flavones of dissimilar chromatogram purification
1, the preparation of standard solution
Precision takes control substance of Rutin 0.025g, dissolves with absolute methanol, and being settled to 100ml, to be made into concentration be 250mg/l, and draw the dilution of this solution 10.0ml absolute methanol and be settled to 100ml, being made into concentration is 25mg/l.
2, the drafting of standard curve and sample tests
The rutin contrast solution 0.00 of accurate absorption 25mg/l, 1.00,2.00,3.00,4.00,5.00,6.00ml is placed in 10ml color comparison tube absolute methanol and is settled to scale, in the uv absorption of 357nm place bioassay standard solution and sample, obtain standard curve regression equation.General flavone content in sample is gone out by regression equation calculation.
3, preparation to be measured
Extractive of general flavone to be measured all adopts embodiment 1 method to prepare except chromatographic column type difference.
4, experimental result
As shown in table 1, the polyamide type that the present invention adopts can improve the extraction efficiency of total flavones effectively
The dissimilar chromatographic column of table 1 is to the content balance of purification total flavones
| Chromatographic column type | Silica gel | Sephadax～G | Sephadax～LH20 | Polyamide |
| Absorbance | 0.251 | 0.249 | 0.340 | 0.463 |
| Account for raw medicinal herbs content (%) | 1.102 | 1.097 | 1.212 | 1.977 |
The extraction effect contrast of the total flavones that embodiment 5 Different Extraction Method obtains
1, the preparation of standard solution
Precision takes control substance of Rutin 0.025g, and dissolve with absolute methanol, be settled to 100ml, being made into concentration is 250mg/l, draws this solution 10.0ml, and be settled to 100ml with absolute methanol dilution, being made into concentration is 25mg/l.
The drafting of 2 standard curves and sample tests
The rutin contrast solution 0.00 of accurate absorption 25mg/l, 1.00,2.00,3.00,4.00,5.00,6.00ml is placed in 10ml color comparison tube absolute methanol and is settled to scale, in the uv absorption of 357nm place bioassay standard solution and sample, obtain standard curve regression equation.General flavone content in sample is gone out by regression equation calculation.
The preparation of 3 products to be tested
Extractive of general flavone to be measured all adopts embodiment 1 method to prepare except extracting method difference.
4 experimental results
As shown in table 2, the total flavones that the present invention prepares is far above the extraction effect of prior art.
The contrast of the general flavone content that table 2 Different Extraction Method obtains
The foregoing is only preferred embodiment of the present invention, and be not used to limit substantial technological context of the present invention, substantial technological content of the present invention is broadly defined in the right of application, any technology entities that other people complete or method, if with application right define identical, also or a kind of change of equivalence, be all covered by being regarded as among this right.
Claims (6)
1. an extracting method for Fructus Nitraria schoberi extractive of general flavone, is characterized in that, adopts Fructus Nitraria schoberi, after fragmentation, adopts 80% alcohol reflux.
2. an extracting method for Fructus Nitraria schoberi extractive of general flavone, is characterized in that comprising the following steps:
1) select ripe Fructus Nitraria schoberi, screen out silt, clean with clear water;
2) the Fructus Nitraria schoberi cold drying 10 ~ 24h at 5 ~ 30 DEG C will cleaned up;
3) to step 2) Fructus Nitraria schoberi that obtains carries out micronizing, and obtaining particle size range is 1 ~ 75 μm of powder;
4) 50% ~ 80% ethanol adding 5 ~ 8 times amount in Fructus Nitraria schoberi powder step 3) obtained carries out supersound extraction 0.5h ~ 1.5h, period stirs with agitator, filtrate I and filtering residue I is obtained after filtration, 50% ethanol ~ 80% ethanol filtering residue I being reused 5 ~ 8 times amount carries out reflux, extract, 0.5h ~ 1.5h, filtrate II and filtering residue II is obtained after filtration, filtrate I and filtrate II are merged, after concentrating under reduced pressure, obtains the total flavones crude extract part of Fructus Nitraria schoberi;
5) will through pretreated polyamide wet method dress post, get above-mentioned crude extract in the different in flow rate loading of 0.2 ~ 0.8ml/min, with the ethanol of 70% concentration with the different in flow rate eluting of 0.5 ~ 1.5ml/min, eluent is merged, concentrated, be drying to obtain described Fructus Nitraria schoberi extractive of general flavone.
3. the preparation method of Fructus Nitraria schoberi extract according to claim 2, its feature, the preparation method of described Fructus Nitraria schoberi extract is:
1) select ripe Fructus Nitraria schoberi 10kg, screen out silt, clean with clear water;
2) the Fructus Nitraria schoberi cold drying 15h at 15 DEG C will cleaned up;
3) by step 2) Fructus Nitraria schoberi that obtains carries out micronizing, obtains Fructus Nitraria schoberi powder;
4) 70% ethanol adding 5 times amount in Fructus Nitraria schoberi powder step 3) obtained carries out supersound extraction 1h, period stirs with agitator, filtrate I and filtering residue I is obtained after filtration, 70% ethanol filtering residue I being reused 7 times amount carries out reflux, extract, 1h, filtrate II and filtering residue II is obtained after filtration, filtrate I and filtrate II are merged, after concentrating under reduced pressure, obtains the thick Extraction parts of total flavones of Fructus Nitraria schoberi;
5) through pretreated polyamide wet method dress post, above-mentioned crude extract will be got in the flow velocity loading of 0.8ml/min, and with the ethanol of 70% concentration with the different in flow rate eluting of 1.0ml/min, eluent be merged, concentrates, be drying to obtain the extractive of general flavone that purity is higher.
4. the preparation method of Fructus Nitraria schoberi extract according to claim 2, its feature, the preparation method of described Fructus Nitraria schoberi extract is:
1) select ripe Fructus Nitraria schoberi 10kg, screen out silt, clean with clear water;
2) the Fructus Nitraria schoberi cold drying 14h at 16 DEG C will cleaned up;
3) by step 2) Fructus Nitraria schoberi that obtains carries out micronizing, obtains Fructus Nitraria schoberi powder;
4) 70% ethanol adding 6 times amount in Fructus Nitraria schoberi powder step 3) obtained carries out supersound extraction 1.5h, period stirs with agitator, filtrate I and filtering residue I is obtained after filtration, 70% ethanol filtering residue I being reused 8 times amount carries out reflux, extract, 1h, filtrate II and filtering residue II is obtained after filtration, filtrate I and filtrate II are merged, after concentrating under reduced pressure, obtains the thick Extraction parts of total flavones of Fructus Nitraria schoberi;
5) through pretreated polyamide wet method dress post, above-mentioned crude extract will be got in the flow velocity loading of 0.75ml/min, and with the ethanol of 70% concentration with the different in flow rate eluting of 0.9ml/min, eluent be merged, concentrates, be drying to obtain the flavone extract that purity is higher.
5. the preparation method of Fructus Nitraria schoberi extract according to claim 2, its feature, the preparation method of described Fructus Nitraria schoberi extract is:
1) select ripe Fructus Nitraria schoberi 10kg, screen out silt, clean with clear water;
2) the Fructus Nitraria schoberi cold drying 13h at 17 DEG C will cleaned up;
3) to step 2) Fructus Nitraria schoberi that obtains carries out micronizing, obtains Fructus Nitraria schoberi powder;
4) 70% ethanol adding 7 times amount in Fructus Nitraria schoberi powder step 3) obtained carries out supersound extraction 1.2h, period stirs with agitator, filtrate I and filtering residue I is obtained after filtration, 70% ethanol filtering residue I being reused 7 times amount carries out reflux, extract, 1.1h, filtrate II and filtering residue II is obtained after filtration, filtrate I and filtrate II are merged, after concentrating under reduced pressure, obtains the thick Extraction parts of total flavones of Fructus Nitraria schoberi;
5) will through pretreated polyamide wet method dress post, get above-mentioned crude extract in the flow velocity loading of 0.8ml/min, with the ethanol of 70% concentration with the different in flow rate eluting of 0.85ml/min, eluent is merged, concentrated, be drying to obtain the extractive of general flavone that purity is higher.
6. have a Fructus Nitraria schoberi extract for slow down aging, blood sugar lowering, antilipemic healthy function, its special type is, described Fructus Nitraria schoberi method for preparing extractive comprises the following steps:
1) select ripe Fructus Nitraria schoberi, screen out silt, clean with clear water;
2) the Fructus Nitraria schoberi cold drying 10 ~ 24h at 5 ~ 30 DEG C will cleaned up;
3) to step 2) Fructus Nitraria schoberi that obtains carries out micronizing, and obtaining particle size range is 1 ~ 75 μm of powder;
4) 50% ethanol ~ 80% ethanol adding 5 ~ 8 times amount in Fructus Nitraria schoberi powder step 3) obtained carries out supersound extraction 0.5h ~ 1.5h, period stirs with agitator, filtrate I and filtering residue I is obtained after filtration, 50% ethanol ~ 80% ethanol filtering residue I being reused 5 ~ 8 times amount carries out reflux, extract, 0.5h ~ 1.5h, filtrate II and filtering residue II is obtained after filtration, filtrate I and filtrate II are merged, after concentrating under reduced pressure, obtains Fructus Nitraria schoberi crude extract part;
5) will through pretreated polyamide wet method dress post, get above-mentioned crude extract in the different in flow rate loading of 0.2 ~ 0.8ml/min, with the ethanol of 70% concentration with the different in flow rate eluting of 0.5 ~ 1.5ml/min, eluent is merged, concentrated, be drying to obtain the Fructus Nitraria schoberi extractive of general flavone that purity is higher.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103181961A (en) * | 2011-12-30 | 2013-07-03 | 北华大学 | Preparation method of flavonoid medicine for treating frostbite |
| CN104855850A (en) * | 2015-04-28 | 2015-08-26 | 青海省轻工业研究所有限责任公司 | Nitraria tangutorum skin residue extract with anti-fatigue effect and preparation method thereof |
-
2015
- 2015-10-28 CN CN201510710584.6A patent/CN105213442A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103181961A (en) * | 2011-12-30 | 2013-07-03 | 北华大学 | Preparation method of flavonoid medicine for treating frostbite |
| CN104855850A (en) * | 2015-04-28 | 2015-08-26 | 青海省轻工业研究所有限责任公司 | Nitraria tangutorum skin residue extract with anti-fatigue effect and preparation method thereof |
Non-Patent Citations (7)
| Title |
|---|
| 朱景郊等: "《承德赤峰贫困山区开发治理与试验研究》", 30 September 1994 * |
| 樊莲莲: "白刺黄酮的提取分离及有效部位抗氧化作用的研究", 《中国优秀硕士学位论文全文数据库 医药卫生科技辑》 * |
| 樊莲莲等: "维药白刺总黄酮对亚急性衰老模型小鼠抗氧化作用的研究", 《时珍国医国药》 * |
| 王建中等: "《中国野生果树物质资源调查与研究》", 30 April 2015 * |
| 王文等: "唐古特白刺中黄酮类化合物正交提取工艺与体外抗菌活性研究", 《中兽医医药杂志》 * |
| 蔡光先等: "《单味中药超微饮片的质量标准研究》", 31 August 2007 * |
| 黄雨三: "《食品安全评价与流通领域检测体系标准化制度化建设及最新政策法规解读 中》", 30 April 2005 * |
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| CN110693924A (en) * | 2019-10-10 | 2020-01-17 | 南京益唯森生物科技有限公司 | Microwave-assisted extraction process of nitraria tangutorum bobr pulp |
| CN112293735A (en) * | 2020-11-11 | 2021-02-02 | 内蒙古曼德拉生物科技有限公司 | Prinsepia utilis royle granules capable of benefiting smooth and preparation method thereof |
| CN116726060A (en) * | 2023-08-08 | 2023-09-12 | 吉林大学 | Application of nitraria tangutorum bobr and extract thereof in liver-protecting and anti-alcohol products |
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