CN105210974B - It is a kind of to be used for the device of online aquatic livestock Nutrition and Metabolism research immediately - Google Patents
It is a kind of to be used for the device of online aquatic livestock Nutrition and Metabolism research immediately Download PDFInfo
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- CN105210974B CN105210974B CN201510732293.7A CN201510732293A CN105210974B CN 105210974 B CN105210974 B CN 105210974B CN 201510732293 A CN201510732293 A CN 201510732293A CN 105210974 B CN105210974 B CN 105210974B
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- 244000144972 livestock Species 0.000 title claims abstract description 50
- 230000004060 metabolic process Effects 0.000 title claims abstract description 35
- 235000016709 nutrition Nutrition 0.000 title claims abstract description 32
- 230000035764 nutrition Effects 0.000 title claims abstract description 31
- 238000011160 research Methods 0.000 title claims abstract description 25
- 238000012258 culturing Methods 0.000 claims abstract description 46
- 238000003860 storage Methods 0.000 claims abstract description 45
- 238000000034 method Methods 0.000 claims abstract description 36
- 238000001514 detection method Methods 0.000 claims abstract description 34
- 230000008569 process Effects 0.000 claims abstract description 34
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 18
- 239000001301 oxygen Substances 0.000 claims abstract description 18
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 70
- 239000004744 fabric Substances 0.000 claims description 19
- 239000000706 filtrate Substances 0.000 claims description 17
- 238000007599 discharging Methods 0.000 claims description 10
- 238000007405 data analysis Methods 0.000 claims description 7
- 230000000694 effects Effects 0.000 claims description 4
- 238000012545 processing Methods 0.000 claims description 4
- 238000007789 sealing Methods 0.000 claims description 4
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 claims description 3
- 229910001220 stainless steel Inorganic materials 0.000 claims description 3
- 239000010935 stainless steel Substances 0.000 claims description 3
- 235000015097 nutrients Nutrition 0.000 abstract description 17
- 230000008859 change Effects 0.000 abstract description 13
- 230000007246 mechanism Effects 0.000 abstract description 5
- 238000011156 evaluation Methods 0.000 abstract description 4
- 238000000338 in vitro Methods 0.000 abstract description 4
- 230000002503 metabolic effect Effects 0.000 abstract description 3
- 239000000126 substance Substances 0.000 description 17
- 241000252233 Cyprinus carpio Species 0.000 description 13
- 241000157468 Reinhardtius hippoglossoides Species 0.000 description 13
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 11
- 238000009395 breeding Methods 0.000 description 6
- 230000001488 breeding effect Effects 0.000 description 6
- 230000000050 nutritive effect Effects 0.000 description 5
- 238000000926 separation method Methods 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 150000002500 ions Chemical class 0.000 description 4
- 230000001681 protective effect Effects 0.000 description 4
- 230000003044 adaptive effect Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 241000251468 Actinopterygii Species 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 238000010171 animal model Methods 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000037396 body weight Effects 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
- 238000004993 emission spectroscopy Methods 0.000 description 2
- 239000013505 freshwater Substances 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 230000001766 physiological effect Effects 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 230000006641 stabilisation Effects 0.000 description 2
- 238000011105 stabilization Methods 0.000 description 2
- IOVCWXUNBOPUCH-UHFFFAOYSA-N Nitrous acid Chemical compound ON=O IOVCWXUNBOPUCH-UHFFFAOYSA-N 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 235000019728 animal nutrition Nutrition 0.000 description 1
- 230000006399 behavior Effects 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000011981 development test Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002417 nutraceutical Substances 0.000 description 1
- 235000021436 nutraceutical agent Nutrition 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/003—Aquaria; Terraria
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/003—Aquaria; Terraria
- A01K63/006—Accessories for aquaria or terraria
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/04—Arrangements for treating water specially adapted to receptacles for live fish
- A01K63/042—Introducing gases into the water, e.g. aerators, air pumps
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Management, Administration, Business Operations System, And Electronic Commerce (AREA)
Abstract
It is used for the device of online aquatic livestock Nutrition and Metabolism research immediately the invention discloses a kind of, including culturing pool, excrement and feed collection device, oxygen supply device, on-line detecting system, holder device, external connection detection means and background process storage system, culturing pool is cylindrical type cylinder body, bottom is connection excrement and feed collection device below cone, cone;Oxygen supply device and on-line detecting system are located at culturing pool both sides respectively;Holder device is welded in culturing pool both sides;External connection detection means is connected by external connection detection device sensor interface with culturing pool;External connection detection means and on-line detecting system are connected with background process storage system.Apparatus of the present invention can continuously detect immediately aquatic livestock ingest after the change of various nutrients in vitro, so as to obtain the deep understanding for its metabolic mechanism;The reservation situation of each nutrient in the aquatic feeds that aquatic livestock ingests, the nutrient balance of the various aquatic feeds of rapid evaluation can be also detected immediately simultaneously.
Description
Technical field
The present invention relates to a kind of device for being used to study aquatic livestock Nutrition and Metabolism, and in particular to one kind is used for immediately online
The device of aquatic livestock Nutrition and Metabolism research, belongs to aquatic livestock nutrient research equipment technical field.
Background technology
Because aquatic animal is compared with terrestrial animal, all exist in terms of living environment, behaviors and habits and physiological property
Larger difference, thus it is more difficult for its nutrient research, and this has become one of factor of obstruction aquatic animal nutrition research.
Conventional Nutrition and Metabolism research it is general by feeding aquatic livestock after, by measuring the metabolism such as its breathing, excrement
Data, obtain model to assess the mechanism of its Nutrition and Metabolism by data, but these data for obtaining, it is typically all several or
The data at multiple time points, can not represent the continuous metabolism status of aquatic livestock, different researcher's selections complete and accurate
Time point it is different, result of study is often widely different or even on the contrary, therefore aquatic livestock metabolic condition can be by one kind
When the research equipment that shows online will bring the visual field and the open thinking that our Nutrition and Metabolism research becomes apparent from,
It can be good at assessing condition of the aquatic feeds in aquatic animal body intracellular metabolite simultaneously, so as to assess the nutrient balance of aquatic feeds
Property, feed formula level is lifted, feed resource is saved, with huge Social benefit and economic benefit.
The content of the invention
The purpose of the present invention is to overcome the deficiencies in the prior art, and provides a kind of for online aquatic livestock immediately
Nutrition and Metabolism research device, can continuously detect immediately aquatic livestock ingest after the change of various nutrients in vitro so that
Obtain for its metabolic mechanism deeper into understanding;The present invention can also detect that the aquatic products that aquatic livestock ingests is raised immediately simultaneously
The reservation situation of each nutrient in material, the nutrient balance of the various aquatic feeds of rapid evaluation.
The present invention is achieved through the following technical solutions:
It is a kind of to be used for the device of online aquatic livestock Nutrition and Metabolism research immediately, including culturing pool, excrement and feed collection
Device, oxygen supply device, on-line detecting system, holder device, external connection detection means and background process storage system, its feature
It is, the culturing pool is cylindrical type cylinder body, bottom is cone, and bottom cone bottom is connected with excrement and feed is collected
Device;Described oxygen supply device and on-line detecting system are located at the both sides of culturing pool cylindrical type cylinder body respectively;Described branch
Rack device is welded in the both sides of culturing pool cylindrical type cylinder body, and props up the effect that support fixation is played on ground of standing on;Described is outer
Connection detection means is connected by the external connection detection device sensor interface on culturing pool cylindrical type cylinder body with culturing pool;Described is outer
Connection detection means and on-line detecting system are connected with background process storage system.
It is preferred that, described culturing pool, the radius of its cylindrical type cylinder body is 0.5-1m, and a height of 0.5-1m, volume is 0.39-
3.14m3;A height of 0.1m of bottom cone, volume is 0.03-0.21m3;The volume of described culturing pool is 0.42-3.35m3。
It is preferred that, the top of described culturing pool cylindrical type cylinder body includes a pressure buffer hole and input hole;The gas
Pressure cushion hole is round, a diameter of 5mm;The input hole is round and equipped with lid, and lid is furnished with sealing ring, the radius of lid
For 0.15m, the function in input hole is input water, delivers feed and be put into experimental animal and filtrate.
It is preferred that, described excrement and feed collection device are that cone type groove, i.e. top are that cylindrical body, bottom are taper
Body;The inlet valve communicated with culturing pool 1 is installed, middle part, which is provided with, intercepts excrement and residual bait above described cylindrical body
Screen cloth, lower section is provided with water discharging valve, and filtrate collecting pit is placed in the lower section of water discharging valve;Described screen cloth is installed on by support
In cylindrical body, its upper and lower is provided with sealing ring.Excrement and feed collection device are collected separately after can water excrement mixture be separated
Excrement.
It is preferred that, described excrement and the cylindrical body of feed collection device, radius is 75mm, is highly 0.2m, bottom cone
The a height of 0.1m of body;The height of described screen portion is 0.05m, and the mesh number of screen cloth is 60 mesh;The filtrate collecting pit is circle
Shape or square, circular radius are 0.1m, and the square length of side is 0.15m.
It is preferred that, described on-line detecting system is intelligent digital sensing system, is the set of multiple on-line sensors,
Be configured with the detection sensor interface of 5~10 kinds of indexs, and can access the sensing equipment of different bores, sensor with it is described after
Platform processing storage system is connected.
It is preferred that, described oxygen supply device is pure oxygen inflator pump, can control oxygen flow size.
It is preferred that, described holder device is stainless steel cylinder or triangular supports;Described cylinder bracket, radius
For 20mm, triangle straight flange is 55mm, is highly 0.5m.
It is preferred that, described external connection detection means is sample collection tube, can connect large-scale detection device such as Amino acid score
The precision detecting instruments such as analyzer, protein analyzer, ion emission spectroscopy instrument, Ions Absorption spectrometer.
It is preferred that, described background process storage system include culturing pool water quality achievement data analyze and process with storing,
Line detects Data Analysis Services and storage, feed nutrition Data Analysis Services and storage, aquatic livestock Nutrition and Metabolism delta data
Analyzing and processing and storage system etc..
The present invention also provides a kind of application method of the above-mentioned device for being used for instant online aquatic livestock Nutrition and Metabolism research:
(1) appropriate treated breeding water is added into device by putting into hole, and opens oxygen supply device
And on-line detecting system, run 10 minutes, device is in even running state, and the water body in device is in equilibrium-like
State, the sensor of on-line detecting system detects to water body, the various physics of background process storage system instant playback water body
Chemical index.
(2) aquatic livestock for needing to study is put into device by putting into hole, is needed to run certain time according to research,
Make the breeding environment in aquatic livestock adaptive device, the sensor of on-line detecting system detects that background process is deposited to water body
The various physical and chemical index of storage system instant playback water body, draw the basic metabolism data of aquatic livestock.
(3) a certain amount of feed of precise is fed aquatic livestock, input is made as far as possible out of input hole feeding device
Feed can be ingested completely by aquatic livestock completely, the sensor of on-line detecting system is detected to water body, background process
The change of the various physical and chemical index of storage system instant playback water body.
(4) according to different aquatic livestocks, after the regular hour, aquatic livestock starts to discharge excrement, excrement and not
The feed ingested is pooled to the bottom of culturing pool, can be discharged into by the valve of excrement and feed collection device in cylindrical body, excrement
After being intercepted with feed by screen cloth, inlet valve is closed, water discharging valve is opened, remaining water is then discharged into filtrate collecting pit, by screen cloth
The excrement and residual bait of upper separation can carry out the detection of various nutritive indexs, testing result input with freezen protective or directly after taking out
Background process storage system, the water of separation is recovered in culturing pool 1 by putting into hole again after flowing into filtrate collecting pit.
(5) trophonemata in the physical and chemical index and feed that are obtained to background process storage system and in excrement is passed through
Target is analyzed and researched, and obtains the data of aquatic livestock change of various nutriments after feed of ingesting.
The present invention has the following effects that compared with prior art:Dress for instant online aquatic livestock Nutrition and Metabolism research
Put, can continuously detect immediately aquatic livestock ingest after the change of various nutrients in vitro, so as to obtain for its metabolism machine
Reason deeper into understanding.Simultaneously the present invention can also at-once monitor aquatic livestock ingest after aquatic feeds each nutrient in vivo
Reservation situation, the nutrient balance of the various aquatic feeds of rapid evaluation.
Brief description of the drawings
Fig. 1:The present invention is used for the planar structure schematic diagram of the device of online aquatic livestock Nutrition and Metabolism research immediately;
Fig. 2:The present invention is used for the dimensional structure diagram of the device of online aquatic livestock Nutrition and Metabolism research immediately;
Fig. 3:The overall structure diagram of excrement and feed collection device of the present invention;
Wherein:1st, culturing pool;2nd, on-line detecting system;3rd, external connection detection device sensor interface;4th, external connection detection means;
5th, background process storage system;6th, hole is put into;7th, oxygen supply device;8th, pressure buffer hole;9th, lid;10th, excrement and feed
Collection device;11st, holder device;12nd, inlet valve;13rd, cylindrical body;14th, screen cloth;15th, water discharging valve;16th, filtrate collecting pit;
17th, support.
Embodiment
The structure for being used for the device of online aquatic livestock Nutrition and Metabolism research immediately to the present invention is entered below in conjunction with the accompanying drawings
The detailed description of row.
It is a kind of to be used for the device of online aquatic livestock Nutrition and Metabolism research immediately, as depicted in figs. 1 and 2, including culturing pool
1st, excrement and feed collection device 10, oxygen supply device 7, on-line detecting system 2, holder device 11, the and of external connection detection means 4
Background process storage system 5:Culturing pool 1 is cylindrical type cylinder body, and bottom is cone, and bottom cone bottom is connected with excrement
And feed collection device;Oxygen supply device 7 and on-line detecting system 2 are located at the both sides of the cylindrical type cylinder body of culturing pool 1 respectively;Branch
Rack device 11 is welded in the both sides of culturing pool cylindrical type cylinder body, and props up the effect that support fixation is played on ground of standing on;Outer joint inspection
Device 4 is surveyed by the external connection detection device sensor interface 3 on culturing pool cylindrical type cylinder body with culturing pool to be connected;Described external connection
Detection means and on-line detecting system are connected with background process storage system.
The radius of the cylindrical type cylinder body of culturing pool 1 is 0.5-1m, and a height of 0.5-1m, volume is 0.39-3.14m3;Justify bottom
A height of 0.1m of cone, volume is 0.03-0.21m3;The volume of culturing pool is 0.42-3.35m3.The cylindrical type cylinder body of culturing pool
Top include pressure buffer hole 8 and input hole 6, pressure buffer hole is round, a diameter of 5mm;Put into hole for it is round simultaneously
Equipped with lid 9, radius is 0.15m, and the function in input hole is input water, delivers feed and be put into experimental animal and filtrate.
Excrement is collected separately after can water excrement mixture be separated in excrement and feed collection device 10, excrement and feed collection device
Just, as shown in Figure 3:It is that cylindrical body 13, bottom are bullet for cone type groove, i.e. top, the radius of cylindrical body is 75mm, height
For 0.2m, a height of 0.1m of bottom pyramidal body;The inlet valve 12 communicated with culturing pool 1 is installed, middle part is installed above cylindrical body
There is the screen cloth 14 for intercepting excrement and residual bait, lower section is provided with water discharging valve 15, and filtrate collecting pit is placed in the lower section of water discharging valve
16;Screen cloth is installed in cylindrical body by support 17.The height of screen portion is 0.05m, and the mesh number of screen cloth is 60 mesh, and filtrate is received
It is circular or square to collect pond 16, and circular radius is 0.1m, and the square length of side is 0.15m.
On-line detecting system 2 is intelligent digital sensing system, is the set of multiple on-line sensors, is configured with 5~10
The detection sensor interface of index is planted, and can access the sensing equipment of different bores, sensor is stored with described background process
System 5 is connected.Oxygen supply device 7 is pure oxygen inflator pump, can control oxygen flow size.Holder device 11 is stainless steel cylinder
Body or triangular supports;Cylinder bracket, radius is 20mm, and triangle straight flange is 55mm, is highly 0.5m.External connection detection means
4 be sample collection tube, can connect large-scale detection device for example amino-acid analyzer, protein analyzer, ion emission spectroscopy instrument,
The precision detecting instruments such as Ions Absorption spectrometer.Background process storage system 5 is analyzed and processed including culturing pool water quality achievement data
With storage, on-line checking Data Analysis Services and storage, feed nutrition Data Analysis Services and storage, aquatic livestock Nutrition and Metabolism
Delta data analyzing and processing and storage system etc..
The present invention is lived in water according to aquatic livestock, the characteristics of various nutriments are uniformly distributed in water, with reference to water
The Nutrition and Metabolism moment of animal is produced in the physiological property of change, passes through various nutrition in the instant on-line checking water body of biology sensor
The changes of contents of material, the change of various nutrients in vitro after reflection aquatic livestock ingests immediately, so as to obtain for its generation
Thank mechanism deeper into understanding;The reservation of each nutrient in the aquatic feeds that aquatic livestock ingests can also be detected immediately simultaneously
Situation, the nutrient balance of the various aquatic feeds of rapid evaluation.On-line detecting system 2 has 5-10 interface, can be flexible
Selection needs the index detected, and science is again convenient;External connection detection means 4 can be connected with large-scale precision instrument, and future can enter
Row device upgrade, sophisticated equipment is selectively accessed according to different research purposes, plays bigger value.Excrement and feed are received
Collecting system 10 be cone groove, be conducive to the concentration of excrement and residual bait, be discharged into by inlet valve 12 in cylindrical body 13, excrement and
After residual bait is intercepted by screen cloth 14, inlet valve 12 is closed, water discharging valve 15 is opened, remaining water is then discharged into filtrate collecting pit 16,
The detection of various nutritive indexs, detection knot can be carried out after the excrement separated on screen cloth and residual bait are taken out with freezen protective or directly
Fruit input background process storage system 5, the water of separation is recovered to culturing pool by putting into hole 6 again after flowing into filtrate collecting pit 16
In 1, it is ensured that nutriment does not lose.Background process storage system 5 is mainly analyzed and processed to data, forms intuitively chart
And statistical result, facilitate researcher to use, while can also be connected with internet, meet user to information-based demand.
With reference to specific embodiment to the device for being used for instant online aquatic livestock Nutrition and Metabolism research of the invention
Aquatic livestock application method of various nutriment changes after feed of ingesting is estimated to be described in detail.
Embodiment 1:
By taking nutrient N change as an example, using apparatus of the present invention after cultured freshwater fish carp feeds feed its body
Interior N retains the calculating of situation and assesses the utilization rate of feed.
(1) appropriate treated cultivation fresh water 100L is added into device by putting into hole 6, and opens oxygen confession
Device 7 and on-line detecting system 2 are answered, is run 10 minutes, device is in even running state, and be in the water body in device
Poised state, the sensor of on-line detecting system detects to water body, the instant playback water body of background process storage system 5 it is each
Plant physical and chemical index;After stable, computer shows the every chemical index of water body, and wherein water body background ammonia nitrogen (Na0) is 0.25mg/
L, background cultured water (Nb0) is 0.02mg/L.
(2) 10 body weight about 50g carp is put into device by putting into hole, the timing of operation one is needed according to research
Between, make the breeding environment in aquatic livestock adaptive device, the sensor of on-line detecting system is detected to water body, background process
The various physical and chemical index of storage system instant playback water body, draw the basic metabolism data of aquatic livestock;Through in half an hour
Stabilization after, computer show carp be put into device water body ammonia nitrogen (Na1) and cultured water (Nb1) after half an hour value difference
For:0.26mg/L and 0.021mg/L.The basic metabolism ammonia nitrogen of Na1 and Na0 difference is carp in device half an hour
(Na):The basic metabolism cultured water (Nb) of 0.01mg/L, Nb1 and Nb0 difference are carp in device half an hour:
0.001mg/L。
(3) by the 7g carp commercial particle feeds of precise out of input hole feeding device, the feed put into 5 minutes
Ingested completely by carp, the sensor of on-line detecting system is detected to water body, background process storage system instant playback water
The change of the various physical and chemical index of body;According to detection data display, carp is after feeding 2 hours, and water body items chemistry refers to
Mark is stable, and excrement is drained, the ammonia nitrogen (Naw) and nitrous acid of data display now, i.e. carp in water body after feeding 2 hours
The value of nitrogen (Nbw) is respectively:0.9mg/L and 0.075mg/L.
(4) carp discharges excrement after ingesting 2 hours, and excrement is pooled to the bottom of culturing pool, can be received by excrement and feed
The valve 12 of acquisition means 10 is discharged into cylindrical body 13, after excrement and feed are intercepted by screen cloth 14, closes inlet valve 12, the row of opening
Penstock 15, remaining water is then discharged into filtrate collecting pit 16, and freezen protective is to concentrate inspection after the excrement separated on screen cloth is taken out
Survey every nutritive index, testing result input background process storage system, the N content (Nf) as in excrement, the current of separation
It is recovered to again by putting into hole in culturing pool 1 after entering in filtrate collecting pit;By detection, the No (Nf) in excrement is 0.182g,
N (NF) is 0.3584g in feed, and is inputted background process storage system (5).
(5) trophonemata in the physical and chemical index and feed that are obtained to background process storage system and in excrement is passed through
Target is analyzed and researched, and obtains the data of aquatic livestock change of various nutriments after feed of ingesting.N reservation in carp body
Measuring (NT) calculation formula is:NT=V*Na0+V*Nb0+NF-Nf-V*Naw-V*Nbw+V*Na*2*h+V*Nb*2*h.(V:Device
Interior water volume;h:From fish is begun place into the hourage for having collected excrement)
In the present embodiment, in carp body N reserved (unit g):NT=V*Na0/1000+V*Nb0/1000+NF-
Nf-V*Naw/1000-V*Nbw/1000+V*Na*2*h/1000+V*Nb*2*h/1000=100 * 0.25/1000+100*0.02/
1000+0.3584-0.182-100*0.9/1000-100*0.075/1000+100*0.01*2*2.5/1000+100*0.001*
2*2.5/1000=0.1114g
In the present embodiment, utilization rate (unit %)=100*NT/NF=of the carp to the N of the commercial feed of experiment
100*0.1114/0.3584=31.08%.According to DIFFERENT FEED N utilization rate, the good job of feed nutrition can be evaluated.
Embodiment 2:
By taking nutrient N change as an example, using apparatus of the present invention after marine fish turbot feeds feed its
Internal N retains the calculating and assessment of situation.
(1) appropriate treated sea area for aquatic breeding water 100L is added into device by putting into hole, and opens oxygen confession
Device and on-line detecting system are answered, is run 10 minutes, device is in even running state, and it is flat to be in the water body in device
Weighing apparatus state, the sensor of on-line detecting system detects to water body, background process storage system instant playback water body it is various
Physical and chemical index;After stable, computer shows the every chemical index of water body, and wherein water body background ammonia nitrogen (Na0) is 0.167mg/
L, background cultured water (Nb0) is 0.013mg/L.
(2) 10 body weight about 50g turbot is put into device by putting into hole, the timing of operation one is needed according to research
Between, make the breeding environment in aquatic livestock adaptive device, the sensor of on-line detecting system is detected to water body, background process
The various physical and chemical index of storage system instant playback water body, draw the basic metabolism data of aquatic livestock;Through in half an hour
Stabilization after, computer show turbot be put into device water body ammonia nitrogen (Na1) and cultured water (Nb1) after half an hour value divide
It is not:0.179mg/L and 0.014mg/L.The basic metabolism ammonia of Na1 and Na0 difference is turbot in device half an hour
Nitrogen (Na):The basic metabolism cultured water (Nb) of 0.012mg/L, Nb1 and Nb0 difference are turbot in device half an hour:
0.001mg/L。
(3) by the 7g turbot commercial particle feeds of precise out of input hole feeding device, the feeding put into 5 minutes
Material is ingested by turbot completely, and the sensor of on-line detecting system is detected to water body, and background process storage system shows immediately
Show the change of the various physical and chemical index of water body;According to detection data display, turbot is after feeding 4 hours, and water body is every
Chemical index is stable, and excrement is drained, the ammonia nitrogen (Naw) of data display now, i.e. turbot in water body after feeding 4 hours
Value with cultured water (Nbw) is respectively:0.69mg/L and 0.055mg/L.
(4) turbot discharges excrement after ingesting 4 hours, and excrement is pooled to the bottom of culturing pool, can pass through excrement and feed
The valve of collection device is discharged into cylindrical body, after excrement and feed are intercepted by screen cloth, closes inlet valve, opens water discharging valve,
Remaining water is then discharged into filtrate collecting pit, and freezen protective is with the every trophonemata of centralized detecting after the excrement separated on screen cloth is taken out
Mark, testing result input background process storage system, the N content (Nf) as in excrement, the water of separation flows into filtrate collecting pit
In after be recovered to again by putting into hole in culturing pool 1.By detection, the No (Nf) in excrement is that N (NF) is in 0.326g, feed
0.5936g, and it is inputted background process storage system.
(5) trophonemata in the physical and chemical index and feed that are obtained to background process storage system and in excrement is passed through
Target is analyzed and researched, and obtains the data of aquatic livestock change of various nutriments after feed of ingesting.N guarantor in turbot body
Allowance (NT) calculation formula is:NT=V*Na0+V*Nb0+NF-Nf-V*Naw-V*Nbw+V*Na*2*h+V*Nb*2*h.(V:Dress
Put interior water volume;h:From fish is begun place into the hourage for having collected excrement)
In the present embodiment, in turbot body N reserved (unit g):NT=V*Na0/1000+V*Nb0/1000+NF-
Nf-V*Naw/1000-V*Nbw/1000+V*Na*2*h/1000+V*Nb*2*h/1000=100 * 0.167/1000+100*
0.013/1000+0.5936-0.326-100*0.69/1000-100*0.055/1000+100*0.012*2*4.5/1000+
100*0.001*2*4.5/1000=0.2228g
In the present embodiment, utilization rate (unit %)=100*NT/NF=of the turbot to the N of the commercial feed of experiment
100*0.2228/0.5936=37.53%.According to DIFFERENT FEED N utilization rate, the good job of feed nutrition can be evaluated.
Examined by development test, when this device for being used for online aquatic livestock Nutrition and Metabolism research immediately obtains data
Between it is short, the related nutritional data of aquatic livestock can be obtained during on-test, it is to avoid long-term cultivation examination in conventional nutraceutical experiment
Test when could obtain sample after end and tested and analyzed, nutritive loss and analytical error that sample long-time storage is brought,
Decrease the error that the selection of the different sampling time points of different researchers is caused.The data obtained continuity is strong, can see constantly
The progress of experiment is observed, Analysis of Nutritive Status of the aquatic livestock in breeding process is fully understanded, analyzes its Nutrition and Metabolism
Mechanism.During system operation, physical index and chemical index can be detected simultaneously, and various physical and chemical indexs pair can be presented immediately
The influence of aquatic livestock Nutrition and Metabolism, scientific basis is provided for production practices.
Examples detailed above is technical concept and technical characterstic to illustrate the invention, can not limit the present invention's with this
Protection domain.Equivalent transformation or modification that all essence according to the present invention is done, should all cover in protection scope of the present invention
Within.
Claims (4)
1. a kind of be used for the device of online aquatic livestock Nutrition and Metabolism research immediately, including culturing pool (1), excrement and feed are collected
At device (10), oxygen supply device (7), on-line detecting system (2), holder device (11), external connection detection means (4) and backstage
Manage storage system (5), it is characterised in that the culturing pool is cylindrical type cylinder body, and bottom is cone, bottom cone bottom
It is connected with excrement and feed collection device;Described oxygen supply device (7) and on-line detecting system are located at culturing pool respectively to be justified
The both sides of column type cylinder body;Described holder device is welded in the both sides of culturing pool cylindrical type cylinder body, and branch is stood on ground and played
Support fixed effect;Described external connection detection means (4) is sensed by the external connection detection device on culturing pool cylindrical type cylinder body
Device interface (3) is connected with culturing pool;Described external connection detection means and on-line detecting system with background process storage system phase
Even;
The top of described culturing pool (1) cylindrical type cylinder body includes a pressure buffer hole (8) and input hole (6);The air pressure
Cushion hole is round, a diameter of 5mm;The input hole is round and equipped with lid (9), and lid is furnished with sealing ring, the half of lid
Footpath is 0.15m;
Described excrement and feed collection device (10) are that cone type groove, i.e. top are that cylindrical body (13), bottom are bullet;Institute
The inlet valve (12) communicated with culturing pool 1 is installed, middle part, which is provided with, intercepts excrement and residual bait above the cylindrical body stated
Screen cloth (14), lower section is provided with water discharging valve (15), and filtrate collecting pit (16) is placed in the lower section of water discharging valve;Described screen cloth
(14) it is installed on by support (17) in cylindrical body, its upper and lower is provided with sealing ring;
Described on-line detecting system (2) is intelligent digital sensing system, is the set of multiple on-line sensors, is configured with 5
The detection sensor interface of~10 kinds of indexs, and can access the sensing equipment of different bores, sensor and described background process
Storage system (5) is connected;
Described oxygen supply device (7) is pure oxygen inflator pump, can control oxygen flow size;
Described holder device (11) is stainless steel cylinder or triangular supports;Described cylinder bracket, radius is 20mm,
Triangle straight flange is 55mm, is highly 0.5m;
Described external connection detection means (4) is sample collection tube, connects large-scale detection device.
2. device according to claim 1, it is characterised in that described culturing pool (1), the radius of its cylindrical type cylinder body is
0.5-1m, a height of 0.5-1m, volume is 0.39-3.14m3;A height of 0.1m of bottom cone, volume is 0.03-0.21m3;Institute
The volume for the culturing pool stated is 0.42-3.35m 3.
3. device according to claim 1, it is characterised in that described excrement and the cylindrical body of feed collection device (10)
(13), radius is 75mm, is highly 0.2m, a height of 0.1m of bottom pyramidal body;The height of described screen cloth (14) part is
0.05m, the mesh number of screen cloth is 60 mesh;The filtrate collecting pit (16) is circular or square, and circular radius is 0.1m, square
The length of side is 0.15m.
4. device according to claim 1, it is characterised in that described background process storage system (5) includes culturing pool
Water quality index Data Analysis Services and storage, on-line checking Data Analysis Services and storage, feed nutrition Data Analysis Services and
Storage, aquatic livestock Nutrition and Metabolism delta data analyzing and processing and storage system.
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Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5081954A (en) * | 1991-07-26 | 1992-01-21 | Alfred R. Priest | Method of raising fish |
CN2527099Y (en) * | 2002-01-30 | 2002-12-25 | 中国科学院水生生物研究所 | Fish breathe analyzer |
CN2850275Y (en) * | 2005-12-12 | 2006-12-27 | 重庆师范大学 | Respiratory chamber with bait-feeding hole and feces drain hole |
CN205727642U (en) * | 2015-10-30 | 2016-11-30 | 中国水产科学研究院黄海水产研究所 | A kind of device for instant online aquatic animal Nutrition and Metabolism research |
-
2015
- 2015-10-30 CN CN201510732293.7A patent/CN105210974B/en active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5081954A (en) * | 1991-07-26 | 1992-01-21 | Alfred R. Priest | Method of raising fish |
CN2527099Y (en) * | 2002-01-30 | 2002-12-25 | 中国科学院水生生物研究所 | Fish breathe analyzer |
CN2850275Y (en) * | 2005-12-12 | 2006-12-27 | 重庆师范大学 | Respiratory chamber with bait-feeding hole and feces drain hole |
CN205727642U (en) * | 2015-10-30 | 2016-11-30 | 中国水产科学研究院黄海水产研究所 | A kind of device for instant online aquatic animal Nutrition and Metabolism research |
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