CN105203538B - Method and detection pipe based on gold nano-material Visual retrieval freshness of meat - Google Patents
Method and detection pipe based on gold nano-material Visual retrieval freshness of meat Download PDFInfo
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Abstract
The invention discloses a kind of method based on gold nano-material Visual retrieval freshness of meat, it is that the different biogenic amine energy reductive hydrolysis agent presomas discharged using the meat of different freshness generate different reducing substanceses, so as under conditions of silver ion presence, reducing substances is carried out chromogenic reaction with gold nano-material, realize the Visual retrieval of freshness of meat;On this basis, present invention also offers a kind of detection pipe of Visual retrieval freshness of meat, it, which includes being provided with gel, gel in transparent tube body and the transparent lid being connected on body, its lid, contains gold nano-material, reducing agent presoma and silver nitrate;The detection pipe can be used for the conventional quick inspection of meat products freshness or the freshness for indicating meat in shelf life in real time.It can realize that freshness of meat is quick, accurate, detect in real time using the inventive method and detection pipe, and its testing cost is low, easy to operate, and be easy to carry and preserve.
Description
Technical field
The invention belongs to meat products detection technique field, and in particular to one kind is based on gold nano-material Visual retrieval meat
The method and its detection pipe of freshness.
Background technology
The corruption of meat is often as during butchering, processing and circulating etc. being polluted and enzyme by external microbe
Caused by effect.Not only in organoleptic properties, color, elasticity, smell etc. serious change occurs for the meat of corruption, and destroys meat
Nutritional ingredient, simultaneously because microbial life metabolism can produce noxious material, the meat after edible corruption can cause food poisoning.
It is that meat production department and consumer provide a kind of instruction food spoilage from health and the consideration of economic and environment-friendly angle is ensured
The instrument tool of degree is of great significance.
In the related invention patent that is detectable or indicating freshness of meat announced at present, a kind of cold fresh meat is fresh
The detection method of degree(CN 104568933A), it is a kind of based on olfaction visualization detect fish freshness method and device(CN
101936912A), it is a kind of can sensing food freshness packaging material manufacture method(CN 102050273A), a kind of food
Freshness indicator(CN 1662813A)It is that VBN is produced by cold fresh meat(TVB-N)Indicator color is changed
Become, to judge the freshness of cold fresh meat, reach the purpose for predicting cold fresh meat shelf life;Estimation of The Fish Freshness Test paper(CN
104297235A)The principle changed colour also with pH, by detecting fish epidermal mucus pH change, then with standard color comparison card ratio
Compared with judging Estimation of The Fish Freshness;It is a kind of to indicate CO2It is characterized the food freshness instruction card and preparation method of gas(CN
104048958A)It is that CO is absorbed by instruction card2Change colour to judge food freshness.Above patent of invention is all based on traditional
PH Cleaning Principles, prediction food is realized by traditional indicator discoloration such as Bromothymol blue, methyl red, dibromophenolphthalein, bromocresol purple
The purpose of product freshness, but the change of its colourity is narrower, and stability of material is poor.Also some patents of invention provide special survey
Determine instrument, such as a kind of method recognized with predicting freshness of meat(CN 102297930A), a kind of near-infrared spectrum technique quickly examines
The method of measuring tape fish freshness(CN 103424374), a kind of quick, sensitive detection freshness of meat method(CN
104458888A), fresh-water fishes freshness ultrasound measuring instrument and detection method(CN 103983693A), it is a kind of based on EO-1 hyperion into
The fillet Noninvasive Measuring Method of Freshness of picture(CN 103439285A), but these methods be required for use special instrument, by special
Personnel realize detection with the operative skill of specialty, are unfavorable for being widely applied and promote.
The content of the invention
It is an object of the invention to provide a kind of method based on gold nano-material Visual retrieval freshness of meat and its
Detection pipe, it can realize that freshness of meat is quick, accurate, detect in real time, and its testing cost is low, easy to operate, and be easy to
Carry and preserve.
To achieve the above object, the present invention is adopted the following technical scheme that:
A kind of method based on gold nano-material Visual retrieval freshness of meat, it is the meat using different freshness
The different biogenic amine energy reductive hydrolysis agent presomas discharged generate different reducing substanceses, so that the bar existed in silver ion
Under part, reducing substances is set to carry out the golden contracted payment particle of chromogenic reaction, generation different wave length or different-thickness with gold nano-material
And color change is produced, realize the Visual retrieval of freshness of meat.
The meat includes beef, the flesh of fish, chicken, duck and seafood products;
The reducing agent presoma is resorcinol acetate(No. CAS:102-29-4;Molecular structural formula:), acetic acid -4- hydroxylphenyl esters(No. CAS:3233-32-7;Molecular structural formula:)、acetylorcinol(No. CAS:889140-51-6;Molecular structural formula:)Or it is right
Diacetic ester(No. CAS:1205-91-0;Molecular structural formula:);
The gold nano-material is gold nanorods or gold nano grain.
The preparation method of the gold nanorods comprises the following steps:
1)The synthesis of crystal seed:By gold chloride and sodium borohydride solution under cetyl trimethylammonium bromide existence condition it is anti-
30s is answered, then in precipitation 1h at 30 DEG C, crystal seed is obtained;
2)The synthesis of gold nanorods:Under cetyl trimethylammonium bromide existence condition, by gold chloride, silver nitrate, salt
After acid, ascorbic acid are mixed, step 1 is added)Prepared crystal seed, lucifuge stands rear centrifugation overnight, and gained precipitation is disperseed again
In cetyl trimethylammonium bromide solution, gold nanorods solution is obtained;
Step 1)The mol ratio of middle gold chloride and sodium borohydride solution is 1:1-10;
Step 2)Middle gold chloride, silver nitrate solution, hydrochloric acid and ascorbic acid mol ratio are 5:1:200:8;Every 2 mmol salt
The volume range of sour crystal seed used is 0.5-5.0 mL.
The preparation method of the gold nano grain is:Add after gold chloride, be heated under the conditions of magnetic agitation in water
Boiling, is rapidly added citric acid three sodium solution, when solution colour is changed into peony, continues to heat 15-20min, then stops
Heating, naturally cools to room temperature, obtains the gold nano grain solution;
The mol ratio of the trisodium citrate and gold chloride is 2-5:1.
A kind of detection pipe based on gold nano-material Visual retrieval freshness of meat includes transparent tube body and is connected to pipe
It is provided with gel, the gel and contains in the cavity of transparent lid on body, body and lid one closing of formation, the lid
Gold nano-material, reducing agent presoma and silver nitrate.
The preparation method of the detection pipe comprises the following steps:
1)After gold nano-material ultrasonic disperse, it is added in gel solution, mixes jointly with reducing agent presoma, silver nitrate
It is even;
2)Take the gel solution of mixing to move on in lid, post package storage is cooled and shaped at room temperature, the detection pipe is obtained.
The gel is Ago-Gel, aquagel or polyvinyl alcohol gel;
Step 2)Middle reducing agent presoma, silver nitrate, the mol ratio of gel solution are 10-120:1:32;Every 32 μm of ol coagulate
The amount of gold nano-material used in sol solution is 20-300 μ L.
The detection pipe can be used for the conventional quick inspection of meat products freshness or for indicating meat in shelf life in real time
The freshness of class product.
The specific method of the conventional quick detection is:Pipe is closed after adding meat leachate in the body of detection pipe
Lid, or encapsulated together with meat after detection pipe is uncapped, in being placed under room temperature or refrigerated condition after 1-5h, by being coagulated in detection pipe
The change of glue color is to judge the freshness of meat.
The specific method that indicates in real time is:In meat products production process, it is total to after detection pipe is uncapped with meat
With encapsulating, dispatch from the factory, transporting, storing, by the change of gel color in detection pipe to reflect in real time in shelf life during this
The freshness of meat.
The remarkable advantage of the present invention is:The present invention, as developer, can make gel film face using novel nano-material
Color change is more rich, and measurement result is more accurate, it is not necessary to expensive instrument detection, and the fresh of meat is can determine whether by naked eyes
Degree, can be achieved that freshness of meat is quick, accurate, detection, and its testing cost is low, easy to operate in real time, and be easy to carry and
Preserve.
Embodiment
A kind of method based on gold nano-material Visual retrieval freshness of meat, it is the meat using different freshness
The biogenic amine of release is different, and energy reductive hydrolysis agent presoma generates different reducing substanceses, so that in silver ion existence condition
Under, reducing substances and gold nano-material is carried out chromogenic reaction, the golden contracted payment particle of generation different wave length or different-thickness and
Color change is produced, the Visual retrieval of freshness of meat is realized.
The meat includes beef, the flesh of fish, chicken, duck and seafood products;
The reducing agent presoma is resorcinol acetate(No. CAS:102-29-4;Molecular structural formula:), acetic acid -4- hydroxylphenyl esters(No. CAS:3233-32-7;Molecular structural formula:)、
acetylorcinol(No. CAS:889140-51-6;Molecular structural formula:)Or hydroquinone diaeetate(CAS
Number:1205-91-0;Molecular structural formula:);
The gold nano-material is gold nanorods or gold nano grain.
The preparation method of the gold nanorods comprises the following steps:
1)The synthesis of crystal seed:By gold chloride and sodium borohydride solution under cetyl trimethylammonium bromide existence condition it is anti-
30s is answered, then in precipitation 1h at 30 DEG C, crystal seed is obtained;
2)The synthesis of gold nanorods:Under cetyl trimethylammonium bromide existence condition, by gold chloride, silver nitrate, salt
After acid, ascorbic acid are mixed, step 1 is added)Prepared crystal seed, lucifuge stands rear centrifugation overnight, and gained precipitation is disperseed again
In cetyl trimethylammonium bromide solution, gold nanorods solution is obtained.
Step 1)The mol ratio of middle gold chloride and sodium borohydride solution is 1:1-10;
Step 2)Middle gold chloride, silver nitrate solution, hydrochloric acid and ascorbic acid mol ratio are 5:1:200:8;Every 2 mmol salt
The volume range of sour crystal seed used is 0.5-5.0 mL.
The preparation method of the gold nano grain is:Add after gold chloride, be heated under the conditions of magnetic agitation in water
Boiling, is rapidly added citric acid three sodium solution, when solution colour is changed into peony, continues to heat 15-20min, then stops
Heating, naturally cools to room temperature, obtains gold nano grain solution;
The mol ratio of the trisodium citrate and gold chloride is 2-5:1.
A kind of detection pipe based on gold nano-material Visual retrieval freshness of meat includes transparent tube body and is connected to pipe
It is provided with gel, the gel and contains in the cavity of transparent lid on body, body and lid one closing of formation, the lid
Gold nano-material, reducing agent presoma and silver nitrate.
The preparation method of the detection pipe comprises the following steps:
1)After gold nano-material ultrasonic disperse, it is added in gel solution, mixes jointly with reducing agent presoma, silver nitrate
It is even;
2)Take the gel solution of mixing to move on in lid, post package storage is cooled and shaped at room temperature, the detection pipe is obtained.
The gel is Ago-Gel, aquagel or polyvinyl alcohol gel;
Step 2)Middle reducing agent presoma, silver nitrate, the mol ratio of gel solution are 10-120:1:32;Every 32 μm of ol coagulate
The amount of gold nano-material used in sol solution is 20-300 μ L.
The detection pipe can be used for the conventional quick inspection of meat products freshness or for indicating meat in shelf life in real time
The freshness of class product.
The specific method of the conventional quick detection is:Pipe is closed after adding meat leachate in the body of detection pipe
Lid, or encapsulated together with meat after detection pipe is uncapped, in being placed under room temperature or refrigerated condition after 1-5h, by by detection pipe
Gel color and colorimetric card contrast, so as to judge the freshness of meat.
The specific method that indicates in real time is:In meat products production process, it is total to after detection pipe is uncapped with meat
With encapsulating, dispatching from the factory, transporting, storing, by the way that the gel color in detection pipe and colorimetric card are contrasted during this, so that anti-in real time
Mirror the freshness of meat in shelf life.
The preparation method of the colorimetric card is:By the detection pipe made respectively with fresh beef, the flesh of fish, chicken, duck,
Seafood etc. is sealed up for safekeeping together, in being stored under room temperature or 4 DEG C of refrigerated conditions, is taken pictures afterwards at regular intervals, is drawn gel color at any time
Between the colorimetric card that changes;Simultaneously using GB/T 5009.44-2003《Meat and the analysis method of meat products sanitary standard》In it is half micro-
The content that nitriding determines TVB-N under different time is measured, to determine freshness of meat under the corresponding time, so as to obtain different gels
The colorimetric card of color correspondence freshness of meat.
In order that content of the present invention easily facilitates understanding, with reference to embodiment to of the present invention
Technical scheme is described further, but the present invention is not limited only to this.
Embodiment one
The synthesis of gold nanorods:
1)The synthesis of crystal seed:In 9.75 mL cetyl trimethylammonium bromides(0.10 mol/L)In, add 0.25 mL
Gold chloride(9.71 mmol/L), then add 0.3 mL sodium borohydride solutions(0.01 mol/L), stir after 30s, at 30 DEG C
Lower precipitation 1h, obtains the crystal seed of dark brown;
2)The synthesis of gold nanorods:In 100 mL cetyl trimethylammonium bromides(0.10 mol/L)In, add 5.15
ML gold chlorides(9.7 mmol/L), 0.1 mL silver nitrate solutiones(0.10 mol/L), 2 mL hydrochloric acid solutions(1.00 mol/L)、
0.8 mL ascorbic acid(0.10 mol/L), 0.5 mL crystal seeds are added after mixing, lucifuge stands rear centrifugation overnight(12000 rpm,
20min), gained precipitation be dispersed in 150.00 mL cetyl trimethylammonium bromide solutions again(33.00 mmol/L)In, obtain
Gold nanorods solution.
Embodiment two
The synthesis of gold nanorods:
1)The synthesis of crystal seed:In 9.75 mL cetyl trimethylammonium bromides(0.10 mol/L)In, add 0.25 mL
Gold chloride(9.71 mmol/L), then add 1.2 mL sodium borohydride solutions(0.02 mol/L), stir after 30s, at 30 DEG C
Lower precipitation 1h, obtains the crystal seed of dark brown;
2)The synthesis of gold nanorods:In 100 mL cetyl trimethylammonium bromides(0.10 mol/L)In, add 5.15
ML gold chlorides(9.71 mmol/L), 0.1 mL silver nitrate solutiones(0.10 mol/L), 2 mL hydrochloric acid solutions(1.00 mol/L)、
0.8 mL ascorbic acid(0.10 mol/L), 0.5 mL crystal seeds are added after mixing, lucifuge stands rear centrifugation overnight(12000 rpm,
20min), gained precipitation be dispersed in 150.00 mL cetyl trimethylammonium bromide solutions again(33.00 mmol/L)In, obtain
Gold nanorods solution.
Embodiment three
The synthesis of gold nano grain:
By 1 mL gold chlorides(24.4 mmol/L)It is added in the conical flask equipped with 100 mL water, adds under magnetic stirring
Heat is rapidly added 2.5 mL citric acid three sodium solution to seething with excitement(20.00 mmol/L), now the color of solution can occur soon
Speed change, to colourless peony is finally presented by faint yellow again to purple, then proceedes to stop heating after heating 15min, nature
Room temperature is cooled to, gold nano grain solution is obtained.
Example IV
The synthesis of gold nano grain:
By 1 mL gold chlorides(24.4 mmol/L)It is added in the conical flask equipped with 100 mL water, adds under magnetic stirring
Heat is rapidly added 2.5 mL citric acid three sodium solution to seething with excitement(48.00 mmol/L), now the color of solution can occur soon
Speed change, to colourless peony is finally presented by faint yellow again to purple, then proceedes to stop heating after heating 15min, nature
Room temperature is cooled to, gold nano grain solution is obtained.
Embodiment five
Response of the gold nanorods to various common biogenic amines:
The μ L of gold nanorods solution 20 prepared by Example one, add 3 μ L silver nitrate solutiones(0.05 mol/L), then
Add 25 μ L resorcinol acetate solution(0.06 mol/L)Mix, be eventually adding 1.80 different mmol/L of 300 μ L
Alkamines(Blank sample, tyrasamine, histamine, spermidine, spermine, ethylenediamine, tryptamines, β-phenyl ethylamine, cadaverine, trimethylamine), place
After 2h, solution colour is respectively:Pink, yellow, light yellow, turquoise, large red, light green-gray in color, light yellow, yellow green,
It is celadon, light yellow.
Embodiment six
Response of the gold nanorods to various common biogenic amines:
The μ L of gold nanorods solution 100 prepared by Example two, add 3 μ L silver nitrate solutiones(0.05 mol/L), so
After add 25 μ L acetylorcinol solution(0.40 mol/L)Mix, be eventually adding 1.80 different mmol/L of 300 μ L
Alkamines(Blank sample, tyrasamine, histamine, spermidine, spermine, ethylenediamine, tryptamines, β-phenyl ethylamine, cadaverine, trimethylamine), place
After 4h, solution colour is respectively:Pink, yellow green, yellow, ashy green color, large red, kermesinus, yellow, green, eucalyptus
Color, yellow green.
Embodiment seven
Response of the gold nano grain to various common biogenic amines:
The μ L of gold nano grain solution 200 prepared by Example three, add 3 μ L silver nitrate solutiones(0.05 mol/L),
Then 25 μ L acetic acid -4- hydroxylphenyl ester solution are added(0.40 mol/L)Mix, be eventually adding 300 μ L different 1.80
Mmol/L alkamineses(Blank sample, tyrasamine, histamine, spermidine, spermine, ethylenediamine, tryptamines, β-phenyl ethylamine, cadaverine, front three
Amine), place after 1h, solution colour be respectively pink, orange red, orange-yellow, yellow, buff, yellow, orange-yellow, yellow,
It is yellow, orange red.
Embodiment eight
Response of the gold nano grain to various common biogenic amines:
The μ L of gold nano grain solution 300 prepared by Example four, add 3 μ L silver nitrate solutiones(0.05 mol/L),
Then 25 μ L hydroquinone diaeetate's solution are added(0.72 mol/L)Mix, be eventually adding 300 μ L different 1.80
Mmol/L alkamineses(Blank sample, tyrasamine, histamine, spermidine, spermine, ethylenediamine, tryptamines, β-phenyl ethylamine, cadaverine, front three
Amine), place 3h after, solution colour be respectively pink, yellow, yellow, buff, buff, buff, yellow, buff,
Buff, yellow.
Embodiment nine
The preparation of detection pipe based on gold nanorods:
1)Gold nanorods are pre-processed:It is standby after gold nanorods solution ultrasound prepared by embodiment one;
2)The assembling of detection pipe:Gold nanorods solution obtained by taking 200 μ L, the agarose for being added to 2 mL water-bath temperature controls coagulates
Glue(15.86 mmol/L)In, 5 μ L resorcinol acetate solution are added after mixing(2.00 mol/L), add 20 μ L
Silver nitrate solution(0.05 mol/L)Mix;
3)The shaping of detection pipe:Take 100 μ L mixed liquors to move on in lid, 5min is placed at room temperature and is allowed to cool after shaping
Encapsulation is produced, then by it in storage at 4 DEG C.
Embodiment ten
The preparation of detection pipe based on gold nano grain:
1)Gold nano grain is pre-processed:It is standby after gold nano grain solution ultrasound prepared by embodiment two;
2)The assembling of detection pipe:Gold nano grain solution obtained by taking 200 μ L, is added to the chitosan of 2 mL water-bath temperature controls
Gel(15.86 mmol/L)In, 30 μ L acetylorcinol solution are added after mixing(2.00 mol/L), add 20 μ
L silver nitrate solutiones(0.05 mol/L)Mix;
3)The shaping of detection pipe:Take 100 μ L mixed liquors to move on in lid, 5min is placed at room temperature and is allowed to cool after shaping
Encapsulation is produced, then by it in storage at 4 DEG C.
Embodiment 11
The preparation of detection pipe based on gold nanorods:
1)Gold nanorods are pre-processed:It is standby after gold nanorods solution ultrasound prepared by embodiment one;
2)The assembling of detection pipe:Gold nanorods solution obtained by taking 200 μ L, is added to the polyvinyl alcohol of 2 mL water-bath temperature controls
In gel(15.86 mmol/L), 60 μ L hydroquinone diaeetate's solution are added after mixing(2.00 mol/L), add 20
μ L silver nitrate solutiones(0.05 mol/L)Mix;
3)The shaping of detection pipe:Take 100 μ L mixed liquors to move on in lid, 5min is placed at room temperature and is allowed to cool after shaping
Encapsulation is produced, then by it in storage at 4 DEG C.
Embodiment 12
The preparation of detection pipe based on gold nanorods:
1)Gold nanorods are pre-processed:It is standby after gold nanorods solution ultrasound prepared by embodiment two;
2)The assembling of detection pipe:Gold nanorods solution obtained by taking 200 μ L, is added to the polyvinyl alcohol of 2 mL water-bath temperature controls
Gel(15.86 mmol/L)In, 30 μ L acetic acid -4- hydroxylphenyl ester solution are added after mixing(2.00 mol/L), add 20
μ L silver nitrate solutiones(0.05 mol/L)Mix;
3)The shaping of detection pipe:Take 100 μ L mixed liquors to move on in lid, 5min is placed at room temperature and is allowed to cool after shaping
Encapsulation is produced, then by it in storage at 4 DEG C.
Embodiment 13
Detection pipe 2 prepared by Example nine, preservative film encapsulation is reinstated after uncapping with 2 parts of beef one of grade weight respectively,
Then 2 parts of beef are recorded into the gel in detection pipe respectively at being placed under room temperature and 4 DEG C of refrigerated conditions when placement 1,2,4,7d
Color, and judge according to the colorimetric card of preparation the freshness of beef, its result is shown in Tables 1 and 2 respectively.
Freshness result after the beef room temperature of table 1 storage different time
Freshness result after the beef of table 2 refrigeration storage different time
Embodiment 14
Detection pipe 2 prepared by Example ten, 2 portions of flesh of fish one after uncapping respectively with grade weight reinstate preservative film encapsulation,
Then 2 portions of flesh of fish are recorded into the gel in detection pipe respectively at being placed under room temperature and 4 DEG C of refrigerated conditions when placement 1,2,4,7d
Color, and the freshness for judging to oppress according to the colorimetric card of preparation, its result are shown in Table 3 and table 4 respectively.
Freshness result after the flesh of fish room temperature storage different time of table 3
Freshness result after the flesh of fish refrigeration storage different time of table 4
Embodiment 15
Detection pipe 8 prepared by Example 11,8 portions of chicken one after uncapping respectively with the equivalent of storage different number of days
Preservative film encapsulation is reinstated, chicken is then recorded into detection respectively at taking out detection pipe after placement 2h under room temperature and 4 DEG C of refrigerated conditions
Gel color in pipe, and judge according to the colorimetric card of preparation the freshness of chicken, its result is shown in Table 5 and table 6 respectively.
Freshness result after the chicken room temperature of table 5 storage different time
Freshness result after the chicken of table 6 refrigeration storage different time
Embodiment 16
Detection pipe 8 prepared by Example 12,8 portions of ducks one after uncapping respectively with the equivalent of storage different number of days
Preservative film encapsulation is reinstated, duck is then recorded into detection respectively at taking out detection pipe after placement 3h under room temperature and 4 DEG C of refrigerated conditions
Gel color in pipe, and judge according to the colorimetric card of preparation the freshness of duck, its result is shown in Table 7 and table 8 respectively.
Freshness result after the duck room temperature of table 7 storage different time
Freshness result after the duck of table 8 refrigeration storage different time
The method and detection pipe that the present invention is provided can realize that freshness of meat is quick, accurate, detect in real time, and it is detected
Cost is low, easy to operate, and is easy to carry and preserves.
The foregoing is only presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with
Modification, should all belong to the covering scope of the present invention.
Claims (10)
1. a kind of method based on gold nano-material Visual retrieval freshness of meat, it is characterised in that:Utilize different freshness
The different biogenic amine energy reductive hydrolysis agent presomas that are discharged of meat generate different reducing substanceses, so as to be deposited in silver ion
Under the conditions, reducing substances is carried out chromogenic reaction with gold nano-material, realize the Visual retrieval of freshness of meat;
The reducing agent presoma is、、Or。
2. the method according to claim 1 based on gold nano-material Visual retrieval freshness of meat, it is characterised in that:Institute
Stating meat includes beef, the flesh of fish, chicken, duck and seafood products;
The gold nano-material is gold nanorods or gold nano grain.
3. the method according to claim 2 based on gold nano-material Visual retrieval freshness of meat, it is characterised in that:Institute
The preparation method for stating gold nanorods comprises the following steps:
1)The synthesis of crystal seed:Gold chloride and sodium borohydride solution are reacted under cetyl trimethylammonium bromide existence condition
30s, then in precipitation 1h at 30 DEG C, obtains crystal seed;
2)The synthesis of gold nanorods:Under cetyl trimethylammonium bromide existence condition, by gold chloride, silver nitrate, hydrochloric acid, anti-
After bad hematic acid is mixed, step 1 is added)Prepared crystal seed, lucifuge stands rear centrifugation overnight, and gained precipitation is dispersed in 16 again
In alkyl trimethyl ammonium bromide solution, gold nanorods solution is obtained;
Step 1)In the mol ratio of used gold chloride and sodium borohydride be 1:1-10;
Step 2)Middle gold chloride, silver nitrate, the mol ratio of hydrochloric acid and ascorbic acid are 5:1:200:8;Every 2 mmol hydrochloric acid is added
The amount of crystal seed is 0.5-5.0 mL.
4. the method according to claim 2 based on gold nano-material Visual retrieval freshness of meat, it is characterised in that:Institute
The preparation method for stating gold nano grain is:Added in water after gold chloride, boiling is heated under the conditions of magnetic agitation, it is rapid to add
Enter citric acid three sodium solution, when solution colour is changed into peony, continue to heat 15-20min, then stop heating, it is naturally cold
But to room temperature, gold nano grain solution is obtained;
The mol ratio of trisodium citrate and gold chloride used is 2-5:1.
5. a kind of detection pipe prepared according to detection method described in claim 1, it is characterised in that:The detection pipe includes transparent
Gel is provided with the cavity of body and the transparent lid being connected on body, body and lid one closing of formation, the lid,
Contain gold nano-material, reducing agent presoma and silver nitrate in the gel.
6. detection pipe according to claim 5, it is characterised in that:Its preparation method comprises the following steps:
1)After gold nano-material ultrasonic disperse, it is added in gel solution, mixes jointly with reducing agent presoma, silver nitrate;
2)Take the gel solution of mixing to move on in lid, post package storage is cooled and shaped at room temperature, the detection pipe is obtained.
7. detection pipe according to claim 6, it is characterised in that:The gel is Ago-Gel, aquagel
Or polyvinyl alcohol gel;
Step 2)Middle reducing agent presoma, silver nitrate, the mol ratio of gel solution are 10-120:1:32;Every 32 μm of ol gels are molten
The amount of gold nano-material used in liquid is 20-300 μ L.
8. a kind of application of detection pipe as claimed in claim 5, it is characterised in that:For the conventional quick of meat products freshness
Examine or the freshness for meat products in instruction shelf life in real time.
9. the application of detection pipe according to claim 8, it is characterised in that:The specific method of the conventional quick detection is:
Added in the body of detection pipe and lid is closed after meat sample or its leachate, or sealed together with meat after detection pipe is uncapped
Dress, in being placed under room temperature or refrigerated condition after 1-5h, by the change of gel color in detection pipe to judge the freshness of meat.
10. the application of detection pipe according to claim 8, it is characterised in that:The specific method that indicates in real time is:In meat
In class process of producing product, encapsulate, dispatch from the factory, transport, store jointly with meat after detection pipe is uncapped, pass through detection during this
The change of gel color to reflect the freshness of meat products in shelf life in real time in pipe.
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