CN105193859A - Application of natural oral bacterium transplanting in oral disease treatment - Google Patents
Application of natural oral bacterium transplanting in oral disease treatment Download PDFInfo
- Publication number
- CN105193859A CN105193859A CN201510619689.0A CN201510619689A CN105193859A CN 105193859 A CN105193859 A CN 105193859A CN 201510619689 A CN201510619689 A CN 201510619689A CN 105193859 A CN105193859 A CN 105193859A
- Authority
- CN
- China
- Prior art keywords
- oral
- oral cavity
- bacterium
- spray
- disease
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicinal Preparation (AREA)
Abstract
Provided is an application of natural oral bacterium transplanting in oral disease treatment. Bacterium samples in the oral cavities of healthy people screened through multiple conditions are collected, and probiotics obtained through centrifuging are prepared into a spray and used for treating oral diseases. Antagonism among bacteria is adopted for replacing antibiotics and other drugs, and oral microecological balance is restored to achieve the effect of treating and preventing the oral diseases. Meanwhile, due to the fact that original oral bacterium samples include multiple beneficial bacteria which cannot be cultured, rich flora is achieved, and diversification of oral micro-ecology is facilitated better. The treatment form of the spray is simple and safe, and is suitable for various patients with the oral diseases, and the functions of preventing and treating pulpitis, tooth periapical peridentitis, gingivitis, periodontitis, acute necrotic ulcerative periodontitis, stomatitis and other oral diseases can be achieved.
Description
Technical field
The present invention relates to a kind of natural oral cavity antibacterial and be implanted in application in oral disease therapeutic, by the method for fungus treatment, realize treatment and the protection of oral disease.
Background technology
The change of Oral health behaviours may cause the generation of various oral disease, is the main inducing of pulpitis, tooth periapical periodontitis, gingivitis, periodontitis, acute necrotizing ulcerative periodontitis, stomatitis etc.In the past, considered that Streptococcus mutans was often separated from dental caries patient diseased region and obtained, and thus manage to use antibiotic to be removed the object reaching anticaries, but result produced little effect.Tracing it to its cause is because Streptococcus mutans belongs to one of intraoral normal flora member, wants thoroughly to remove it, and one is that operation easier is large, the original microecological balance in destruction oral cavity that the two can be serious, thus causes dysbacteriosis.Therefore; dental caries and periodontal disease theory are because oral cavity flora imbalance that is normal, symbiosis causes; therefore under the prerequisite of protection Oral health behaviours ecological balance, should carry out treating and taking preventive measures, make it recover the normal importance and functions in biome in treatment.
Antibiotic abuse, likely causes the imbalance of original flora system, thus causes even more serious consequence, cause the appearance of Resistant strain while killing and wounding Streptococcus mutans.Therefore, attempt utilizing probiotics preparation to recover and regulate Oral health behaviours to balance a kind of selection well of can yet be regarded as.And to reach with the fungi-proofing object of bacterium, first will understand distribution and the quantitative transformations of flora in Oral health behaviours system, for the exploitation of later stage Oral health behaviours preparation and the control of opportunist provide basic data.
At present, viable bacteria technology is widely used in the field such as food, medical science as a kind of common technology, but this technology resolution is low, poor repeatability, depend on cultivation, therefore requires that harsher microorganism is difficult to monitor to some growths.Therefore, viable count method is planned in this research and high throughput sequencing technologies combines, from Microflora and kind, the microorganism in normal person oral cavity and patient's mouth is furtherd investigate, for the research and development of the prevention of later stage oral cavity class disease, treatment and mouth cavity medicine provide theoretical foundation.
Summary of the invention
The object of the present invention is to provide a kind of natural oral cavity antibacterial to be implanted in application in oral disease therapeutic, by regulating and recovering human oral cavity Tiny ecosystem, the treatment of multiple oral disease can be realized, and the generation of prevention oral disease that can be permanently effective.
Application process of the present invention comprises the following steps:
1, buccal sample collection and screening conditions: adopt the mode that normal saline is gargled, gather the oral cavity bacterium sample of healthy population, centrifugal 10000rpm/min3min, collect thalline and suspend with normal saline; To oral cavity bacterium picker screening conditions be:
There is not diarrhoea in a, at least closely 3-6 month and do not use antibiotic;
B, without oral disease;
C, bad sexual life is not had to have a liking for;
D, non-communicable disease, as HIV, hepatitis B, hepatitis C etc.;
2, lyophilized powder preparation: by the primitive oral cavity probiotic bacteria of centrifugal acquisition, centrifugal 10000rpm/min3min, brine 5 times, adopts freeze dryer to make lyophilized powder, retains for subsequent use.
3, the preparation of spray: got by oral cavity Freeze-dry Powder of Probioctics and be dissolved in normal saline in right amount, make spray, repeatedly spray into probiotics preparation, be conducive to the propagation of oral cavity probiotic bacteria, recovers Oral health behaviours balance, reaches the effect for the treatment of and prevention oral disease.
Beneficial effect of the present invention: adopt the antagonism substitute antibiotics between antibacterial and other drug, reaches the effect for the treatment of and prevention oral disease by recovering Oral health behaviours balance; Meanwhile, because original oral cavity bacterium sample, containing the multiple beneficial bacteria that cannot cultivate, flora enriches, and is more conducive to the variation of Oral health behaviours; And the form of therapy of spray is simple, safety, is applicable to all kinds of suffering from oral disease, can plays the effect of prevention and therapy as oral diseases such as pulpitis, tooth periapical periodontitis, gingivitis, periodontitis, acute necrotizing ulcerative periodontitis, stomatitis.
Detailed description of the invention
Application process of the present invention comprises the following steps:
1, buccal sample collection and screening conditions: adopt the mode that normal saline is gargled, gather the oral cavity bacterium sample of healthy population, centrifugal 10000rpm/min3min, collect thalline and suspend with normal saline; To oral cavity bacterium picker screening conditions be:
There is not diarrhoea in a, at least closely 3-6 month and do not use antibiotic;
B, without oral disease;
C, bad sexual life is not had to have a liking for;
D, non-communicable disease, as HIV, hepatitis B, hepatitis C etc.;
2, lyophilized powder preparation: by the primitive oral cavity probiotic bacteria of centrifugal acquisition, centrifugal 10000rpm/min3min, brine 5 times, adopts freeze dryer to make lyophilized powder, retains for subsequent use.
3, the preparation of spray: got by oral cavity Freeze-dry Powder of Probioctics and be dissolved in normal saline in right amount, make spray, repeatedly spray into probiotics preparation, be conducive to the propagation of oral cavity probiotic bacteria, recovers Oral health behaviours balance, reaches the effect for the treatment of and prevention oral disease.
Claims (1)
1. natural oral cavity antibacterial is implanted in the application in oral disease therapeutic, it is characterized in that:
(1), buccal sample collection and screening conditions: adopt the mode that normal saline is gargled, gather the oral cavity bacterium sample of healthy population, centrifugal 10000rpm/min3min, collect thalline and suspend with normal saline;
To oral cavity bacterium picker screening conditions be:
There is not diarrhoea in a, at least closely 3-6 month and do not use antibiotic;
B, without oral disease;
C, bad sexual life is not had to have a liking for;
D, non-communicable disease, as HIV, hepatitis B, hepatitis C;
(2), lyophilized powder preparation: by the primitive oral cavity probiotic bacteria of centrifugal acquisition, centrifugal 10000rpm/min3min, brine 5 times, adopt freeze dryer make lyophilized powder, retain for subsequent use;
(3), the preparation of spray: oral cavity Freeze-dry Powder of Probioctics is got and is dissolved in normal saline in right amount, make spray, repeatedly spray into probiotics preparation, be conducive to the propagation of oral cavity probiotic bacteria, recovery Oral health behaviours balances, and reaches the effect for the treatment of and prevention oral disease.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510619689.0A CN105193859A (en) | 2015-09-25 | 2015-09-25 | Application of natural oral bacterium transplanting in oral disease treatment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510619689.0A CN105193859A (en) | 2015-09-25 | 2015-09-25 | Application of natural oral bacterium transplanting in oral disease treatment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105193859A true CN105193859A (en) | 2015-12-30 |
Family
ID=54942049
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510619689.0A Pending CN105193859A (en) | 2015-09-25 | 2015-09-25 | Application of natural oral bacterium transplanting in oral disease treatment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105193859A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102245191A (en) * | 2008-12-16 | 2011-11-16 | 雀巢产品技术援助有限公司 | Compositions and methods for improved oral health |
| CN103119153A (en) * | 2010-08-31 | 2013-05-22 | 公共健康高级研究中心 | Anticaries compositions and probiotics/prebiotics |
| CN103298924A (en) * | 2010-08-18 | 2013-09-11 | Ab－生物学有限公司 | Probiotic composition for oral health |
| CN104825380A (en) * | 2015-04-21 | 2015-08-12 | 溧水区人民医院 | Oral normal flora rapid recovery nursing liquid and preparation method thereof |
-
2015
- 2015-09-25 CN CN201510619689.0A patent/CN105193859A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102245191A (en) * | 2008-12-16 | 2011-11-16 | 雀巢产品技术援助有限公司 | Compositions and methods for improved oral health |
| CN103298924A (en) * | 2010-08-18 | 2013-09-11 | Ab－生物学有限公司 | Probiotic composition for oral health |
| CN103119153A (en) * | 2010-08-31 | 2013-05-22 | 公共健康高级研究中心 | Anticaries compositions and probiotics/prebiotics |
| CN104825380A (en) * | 2015-04-21 | 2015-08-12 | 溧水区人民医院 | Oral normal flora rapid recovery nursing liquid and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| MEURMAN JH ET AL: "Probiotics: do they have a role in oral medicine and dentistry?", 《EUR J ORAL SCI》 * |
| 杨娟: "口腔益生乳酸杆菌的筛选及特性研究", 《中国优秀硕士学位论文全文数据库 医药卫生科技辑》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Maltz et al. | Conventional caries removal and sealed caries in permanent teeth: a microbiological evaluation | |
| Baysan et al. | Antimicrobial effect of a novel ozone–generating device on micro–organisms associated with primary root carious lesions in vitro | |
| CN105685342A (en) | Probiotic buccal tablet and preparation method thereof | |
| El Sherbiny | Control of growth Streptococcus mutans isolated from saliva and dental caries | |
| Bhateja | The miraculous healing therapy–“Ozone therapy” in dentistry | |
| CN105310960B (en) | A kind of novel polysaccharide antimicrobial mouthwash and preparation method thereof | |
| Kramer et al. | The immediate and long-term effects of invasive and noninvasive pit and fissure sealing techniques on the microflora in occlusal fissures of human teeth | |
| CN104244912A (en) | Natural antibacterial toothpaste composition having effects of alleviating oral pain, killing oral bacteria, and calming nerves | |
| CN111000874A (en) | Lactobacillus plantarum with dental caries prevention and treatment effect | |
| CN114606164A (en) | Lactobacillus paracasei Probio-01 and application thereof in preparing probiotic oral preparation | |
| CN109090148A (en) | A kind of refreshment and tranquilization health perfume (or spice) and preparation method thereof | |
| CN107753578A (en) | A kind of quick compound probiotic antibacterial liquid for killing oral cavity virus | |
| CN105193859A (en) | Application of natural oral bacterium transplanting in oral disease treatment | |
| CN102784209B (en) | Traditional Chinese medicine composition for preventing and treating tooth sensitivity, toothpaste and preparation method thereof | |
| CN102727803B (en) | Medicine for treating periodontitis | |
| CN105193857A (en) | Application of transplantation of artificially-cultured oral bacteria in treatment of oral diseases | |
| CN107648136A (en) | Nurse the mouthwash in oral cavity and preparation method thereof | |
| CN106236645A (en) | A kind of natural child's collutory | |
| CN102836277B (en) | Medicine composition for treating periodontal diseases as well as preparation method and application thereof | |
| CN1876030A (en) | A method for preparing toothache-relieving medicine | |
| Farrokhnia et al. | Comparison of the effect of fluorin total mouthwash with chlorhexidine on the growth rate of streptococcus mutans | |
| HARTZELL et al. | The dental path: its importance as an avenue to infection | |
| CN108403753A (en) | The fermented tcm and preparation method thereof of prevention blue otopathy and circovirus | |
| Kitasako et al. | Growth‐Inhibitory Effect of Antibacterial Self‐Etching Primer on Mutans Streptococci Obtained from Arrested Carious Lesions | |
| CN106236648A (en) | A kind of natural child's collutory |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20151230 |