CN105193387A - Method for detecting tooth-tattoo biosensor device for oral sanitation detection - Google Patents
Method for detecting tooth-tattoo biosensor device for oral sanitation detection Download PDFInfo
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- CN105193387A CN105193387A CN201510540625.1A CN201510540625A CN105193387A CN 105193387 A CN105193387 A CN 105193387A CN 201510540625 A CN201510540625 A CN 201510540625A CN 105193387 A CN105193387 A CN 105193387A
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Abstract
The invention discloses a method for detecting a tooth-tattoo biosensor device for oral sanitation detection. The method comprises the following steps: (1) adhering the tooth-tattoo biosensor on an incisor of a detected person; (2) making the detected person relax and move freely to normally salivate; (3) connecting a weak signal detection module with the contact of the tooth-tattoo biosensor after 20 minutes, and measuring the sensor signal; (4) measuring every 10 minutes for 3 times; (5) disconnecting the weak signal detection module with the contact of the sensor, and making the detected person to rapidly run for 5 miles with high strength; (6) connecting the weak signal detection module with the contact of the sensor, and measuring the sensor signal; (7) measuring every 10 minutes for 3 times; (8) allowing the detected person to take a rest for 2 hours, connecting the weak signal detection module with the sensor contact, and measuring the sensor signal; and (9) measuring every 10 minutes for 3 times. The device has the advantages of short detection time and modern appearance, is quantitative and accurate, and can be easily accepted by young people.
Description
Technical field
The present invention relates to field of biomedicine technology, be specifically related to a kind of detection method of oral hygiene detection stricture of vagina tooth (tooth is tatooed) biosensor arrangement.
Background technology
" tooth is tatooed " becomes the trend that smark set competitively imitates recently at home and abroad.A lot of people can select the pattern oneself liked to decorate the tooth of oneself, mark, the cartoon character of certain football team of state such as liked, or the symbol etc. that representative is liked.Tooth is tatooed has become the fashionable new lover leted others have a look at abroad, promotes gradually.
Oral health state is the mirror of reflection life and health quality, and the generation of human body diseases often produces the kind of biomolecule or the change of quantity in vivo, and these changes also can be reflected by oral secretion.Such as, in saliva of buccal cavity, the concentration of glucose and the concentration of glucose in blood are positively related under certain controlled condition, can be assessed the concentration of human body glucose in blood by the concentration detecting glucose in saliva to a certain extent.So the concentration detecting glucose in saliva may be used for glucose controlling extent in examination diabetes patient or assessment diabetes human body.Such as, oral cavity Tooth surface lactic acid content becomes certain relation with dental surface bacterial number, can infer Buccodental surface bacteria quantity by detecting dental surface lactic acid content.Also have, in saliva of buccal cavity, lactic acid content becomes positive correlation with lactic acid content in human body, human body lactic acid content reflection fatigue level of human body or cardiac status, the important indicator passing judgment on athlete's physique, so the physical state of experimenter can be assessed by detecting lactic acid content in saliva of buccal cavity.
Radioimmunoassays (RIAs), high pressure liquid chromatographic analysis method, protein analyzer, various diagnostic kit and immunity-chromatography test strip etc. are had clinically at present to the common detection methods of content of material in saliva, there is radioactive pollution, need large-scale instrument in these methods, length consuming time, cost are high, can not the shortcoming such as detection by quantitative, application clinically has certain limitation.
Summary of the invention
The object of the invention is for overcoming above-mentioned the deficiencies in the prior art, a kind of detection method of oral hygiene detection stricture of vagina tooth biosensor arrangement is provided, this detection method is short for detection time, can realize quantitatively accurately detecting, detecting instrument miniature portable, but also attractive in appearance modish, be easy to youngster and accept.
For achieving the above object, the present invention adopts following technical proposals:
A kind of detection method of oral hygiene detection stricture of vagina tooth biosensor arrangement, described stricture of vagina tooth biosensor arrangement, comprise stricture of vagina tooth biosensor, communication module and Detection of Weak Signals module, communication module is connected with Detection of Weak Signals module with the contact of stricture of vagina tooth biosensor respectively, obtain the output signal of stricture of vagina tooth biosensor, and by Signal transmissions to Detection of Weak Signals module; Described stricture of vagina tooth biosensor comprises sensitive material fixing on substrate, stricture of vagina tooth image, three electrodes combined with stricture of vagina tooth image and electrode, described three electrodes are fixed on the diverse location of the same face on substrate, three electrodes are connected with three contacts being positioned at substrate one edge independent of each other respectively by different contact conductors, that face of substrate that contact conductor is set is coated with stricture of vagina tooth image layer, and three electrodes and three contacts are exposed to outside stricture of vagina tooth image layer;
Described detection method is as follows:
1) the stricture of vagina tooth biosensor prepared carefully is attached on the front tooth of detected person;
2) before ensureing to measure, allow test substance (as lactic acid) in body be in a metastable state, allow detected person loosen, arbitrarily walk about, normal secretions saliva;
3) after 20 minutes, the contact of Detection of Weak Signals module with stricture of vagina tooth biosensor is connected, measuring transducer signal, this signal is initial basic point signal;
4) for preventing exceptional value, measuring once every 10 minutes, measuring 3 times;
5) disconnect the connection of Detection of Weak Signals module and sensor contact, allow detected person's high strength run 5 kilometers fast;
6) then Detection of Weak Signals module is connected with sensor contact, measuring transducer signal, this signal be high-intensity exercise after test substance (as lactic acid) content in saliva;
7) for preventing exceptional value, measuring once every 10 minutes, measuring 3 times;
8) detected person is allowed to have a rest 2 hours, again Detection of Weak Signals module is connected with sensor contact, measuring transducer signal, this signal is a comparison signal, should be lower than test substance after high-intensity exercise (as lactic acid) content for test substance (as lactic acid) content after contrasting rest;
9) for preventing exceptional value, measuring once every 10 minutes, measuring 3 times.
Communication module in the present invention is wirelessly can have two kinds of communication modes, a kind of is by wireless form, the contact of communication module with stricture of vagina tooth biosensor is connected, obtain the output signal of stricture of vagina tooth biosensor, and signal is spread out of in the mode of wireless telecommunications, receiving system obtains the output signal of sensor; Another kind is wired form, is connected the contact of Detection of Weak Signals module with stricture of vagina tooth biosensor, directly obtains the output signal of stricture of vagina tooth biosensor.
Wireless communication mode is measured, and measured arbitrarily can walk about within the specific limits and speak, and does not affect normal activity; Wire communication mode, because have wire by oral cavity by stricture of vagina tooth biosensor and Detection of Weak Signals model calling, possible some effects measured is movable or speak.
Described wireless communication mode is bluetooth, infrared light or radio frequency.
Described wireless communication module is the microstructure that plant teeth crevice is carried with.
Described substrate employing one is harmless and Undec non-biocompatible thin film's material is prepared from oral cavity, such as: nitrocellulose, collagen, gelatin, chitosan, hyaluronate sodium, eggshell membrane, tetrafluoroethene or polylactic acid.
Described three electrodes are that gold or carbon inactive, conductive material are prepared from, and three electrodes are respectively working electrode, reference electrode and the 3rd electrode.
Described contact conductor and contact are made by silver, gold, carbon or silver-colored silver chloride material.
Described sensitive material is formed by the solidification of enzyme, electron mediator and CNT.The enzyme class of fixing have relation with the composition that will survey, such as: measure lactic acid and fix Lactate Oxidase, measure glucose fixing glucose oxidase.
Stricture of vagina tooth biosensor can measure the composition of many kinds of substance in oral cavity, measure different compositions, different enzymes is fixed when making biosensor, such as, utilize the lactic acid content in stricture of vagina tooth biosensor measurement oral cavity, so need fixing Lactate Oxidase when making stricture of vagina tooth sensor; The content of glucose in oral cavity to be measured for another example, so need fixing glucose oxidase when making stricture of vagina tooth sensor.
Described Detection of Weak Signals module comprises central processing element, pin-saving chip, Signal Collection and amplify chip, communication chip, sensor contact interface and peripheral circuit thereof, and pin-saving chip, Signal Collection and amplify chip, communication chip, sensor contact interface and peripheral circuit thereof all communicate with central processing element;
Described central processing element stores the performing a programme of detection module, and performs corresponding instruction and action according to procedure order;
Described pin-saving chip is for storing the measurement result of the stricture of vagina tooth biosensor of detection module acquisition;
Described Signal Collection and amplify chip for gather stricture of vagina tooth biosensor small-signal and by signal processing and amplifying;
Described communication chip is for realizing the connecting communication of Detection of Weak Signals module and host computer;
Described sensor contact interface is connected with the contact of stricture of vagina tooth biosensor, provides voltage and measuring transducer output current to sensor, and wherein the concentration of the size sensor test substance of current output sensor is correlated with.
The present invention caters to youthful trend, a kind of stricture of vagina tooth (tooth is tatooed) biosensor arrangement detected for oral hygiene is disclosed, to detect in saliva compared with material concentration with the large-scale biochemistry analyzer of tradition, this device can overcome problems of the prior art, can realize detecting oral hygiene, do not need large-scale instrument again, detection time of the present invention is short, can realize quantitatively accurately detecting, detecting instrument miniature portable, but also attractive in appearance modish, being easy to youngster and accepting, is a kind of newest form of oral hygiene checkout gear.
Accompanying drawing explanation
Fig. 1 is a stricture of vagina tooth image;
Fig. 2 is three electrodes combined with stricture of vagina tooth image;
Fig. 3 be combined with stricture of vagina tooth image before stricture of vagina tooth biosensor structure schematic diagram;
Fig. 4 is wireless communication module schematic diagram;
Fig. 5 is Detection of Weak Signals module diagram;
Fig. 6 is examples measure result figure;
Wherein, 1. the 3rd electrode, 2. working electrode, 3. reference electrode, 4. contact, 5. contact conductor, 6. substrate, 7. central processing element, 8. pin-saving chip, 9. Signal Collection and amplify chip, 10. communication chip, 11. sensor contact interfaces, 12. peripheral circuits.
Detailed description of the invention
Below in conjunction with drawings and Examples, the present invention is further described.
Lactic acid is a kind of important substance of human body, and human body lactic acid content is relevant with many factors, such as moves, breathes, degree of fatigue, is also the important indicator reflecting cardiac function.After human motion, in saliva of buccal cavity, in lactic acid content and body, lactic acid content has higher dependency.This example measure motion with the present invention after in saliva of buccal cavity the example that is changed to of lactic acid content detected.
The change detecting method that employing the present invention carries out lactic acid content is as follows:
The male detected person that prior invitation one is healthy, the detecting step measuring its oral cavity lactic acid change with the present invention is as follows:
1) the stricture of vagina tooth lactic acid sensor previously prepared carefully is attached on the front tooth of detected person;
2) before ensureing to measure, allow lactic acid in body be in a metastable state, allow detected person loosen, arbitrarily walk about, normal secretions saliva;
3) after 20 minutes, Detection of Weak Signals module is connected with sensor contact, measuring transducer signal, this signal is initial basic point signal;
4) for preventing exceptional value, measuring once every 10 minutes, measuring 3 times;
5) disconnect the connection of Detection of Weak Signals module and sensor contact, allow detected person's high strength run 5 kilometers fast;
6) then Detection of Weak Signals module is connected with sensor contact, measuring transducer signal, this signal be high-intensity exercise after lactic acid content in saliva;
7) for preventing exceptional value, measuring once every 10 minutes, measuring 3 times;
8) allow detected person have a rest 2 hours, then Detection of Weak Signals module be connected with sensor contact, measuring transducer signal, this signal is a comparison signal, should be lower than enzyme content to be measured after high-intensity exercise for enzyme content to be measured after contrasting rest;
9) for preventing exceptional value, measuring once every 10 minutes, measuring 3 times.
Measurement result as shown in Figure 6, the current signal reflection that sensor exports be the content of lactic acid in subjects saliva, as can be seen from Figure 6, after vigorous exercise, in subjects saliva, lactic acid content obviously increases, and after rest after a while, lactic acid content declines again.This is consistent with the result detected by other method.
Stricture of vagina tooth biosensor can measure the composition of many kinds of substance in oral cavity, measure different compositions, different enzymes is fixed when making biosensor, such as, utilize the lactic acid content in stricture of vagina tooth biosensor measurement oral cavity, so need fixing Lactate Oxidase when making stricture of vagina tooth sensor; The content of glucose in oral cavity to be measured for another example, so need fixing glucose oxidase when making stricture of vagina tooth sensor.
The content measuring glucose in oral cavity is identical with the lactic acid content technique that above-described embodiment is measured in oral cavity, and just detected object changes, and does not repeat them here.
The stricture of vagina tooth biosensor arrangement detected for oral hygiene has two kinds of structures, and one is as follows:
As Figure 1-Figure 4, for the stricture of vagina tooth biosensor arrangement that oral hygiene detects, comprise stricture of vagina tooth biosensor, wireless communication module and Detection of Weak Signals module, described wireless communication module has two groups, one group is connected with the contact 4 of stricture of vagina tooth biosensor, obtain the output signal of stricture of vagina tooth biosensor, and signal is spread out of in the mode of wireless telecommunications, another group is connected with Detection of Weak Signals module, identifies the output signal of reception stricture of vagina tooth biosensor and is transferred to Detection of Weak Signals module; As shown in Figure 4.Wireless communication module is bluetooth, infrared light or radio frequency.Wireless communication module is the microstructure that plant teeth crevice is carried with, and does not affect oral cavity normal function.
As shown in Figure 3, stricture of vagina tooth biosensor comprises sensitive material fixing on substrate 6, stricture of vagina tooth image, three electrodes combined with stricture of vagina tooth image and electrode, described three electrodes are fixed on the diverse location of the same face on substrate 6, three electrodes are connected with three contacts 4 being positioned at substrate 6 one edge independent of each other respectively by different contact conductors 5, that face of substrate 6 that lead-in wire is set is coated with stricture of vagina tooth image layer, and three electrodes and three contacts 4 are exposed to outside stricture of vagina tooth image layer.
As shown in Figure 1, what stricture of vagina tooth image adopted is a kind of harmless, not decompose in oral cavity and to keep the material of its CF, this kind of material is existing technology, all can buy in the market, belongs to known technology, does not repeat them here.
Substrate 6 adopts a kind of harmless and Undec non-biocompatible thin film's material is prepared from oral cavity, such as: nitrocellulose, collagen, gelatin, chitosan, hyaluronate sodium, eggshell membrane, tetrafluoroethene or polylactic acid.
Three electrodes are that gold or carbon inactive, conductive material are prepared from, and three electrodes are respectively working electrode 2, reference electrode 3 and the 3rd electrode 1.As shown in Figure 2.
Contact conductor 5 and contact are made by silver, gold, carbon or silver-colored silver chloride material.Manufacture method is sputtering, evaporation or printing.
Sensitive material is formed by the solidification of enzyme, electron mediator and CNT.Electrode fixing sensitive material, such as enzyme, is to make sensor have specificity, only reacts with a kind of composition (such as lactic acid) in oral cavity or only detects concentration (such as oral cavity bacterium quantity) that is a kind of or a class material; Fixing nano-particle is the sensitivity in order to increase sensor, improves the sensitivity detected.
Detection of Weak Signals module comprises central processing element 7, pin-saving chip 8, Signal Collection and amplify chip 9, communication chip 10, sensor contact interface 11 and peripheral circuit 12 thereof; Pin-saving chip 8, Signal Collection and amplify chip 9, communication chip 10, sensor contact interface 11 and peripheral circuit 12 thereof all communicate with central processing element 7;
Central processing element 7 stores the performing a programme of detection module, and performs corresponding instruction and action according to procedure order;
Pin-saving chip 8 is for storing the measurement result of the stricture of vagina tooth biosensor of detection module acquisition;
Signal Collection and amplify chip 9 for gather stricture of vagina tooth biosensor small-signal and by signal processing and amplifying;
Communication chip 10 is for realizing the connecting communication of Detection of Weak Signals module and host computer;
Sensor contact interface 11 is connected with the contact 4 of stricture of vagina tooth biosensor, provides voltage and measuring transducer output current to sensor, and wherein the concentration of the size sensor test substance of current output sensor is correlated with.
Its two structure for the stricture of vagina tooth biosensor arrangement of oral hygiene detection is as follows:
As shown in Fig. 1-Fig. 3, Fig. 5, for the stricture of vagina tooth biosensor arrangement that oral hygiene detects, comprise stricture of vagina tooth biosensor, communication module and Detection of Weak Signals module, communication module adopts wired connection mode, as shown in Figure 5, be directly connected with Detection of Weak Signals module by the contact of data wire by stricture of vagina tooth biosensor.
As shown in Figure 3, stricture of vagina tooth biosensor comprises sensitive material fixing on substrate 6, stricture of vagina tooth image, three electrodes combined with stricture of vagina tooth image and electrode, described three electrodes are fixed on the diverse location of the same face on substrate 6, three electrodes are connected with three contacts 4 being positioned at substrate 6 one edge independent of each other respectively by different contact conductors 5, that face of substrate 6 that lead-in wire is set is coated with stricture of vagina tooth image layer, and three electrodes and three contacts 4 are exposed to outside stricture of vagina tooth image layer.
As shown in Figure 1, what stricture of vagina tooth image adopted is a kind of harmless, not decompose in oral cavity and to keep the material of its CF, this kind of material is existing technology, all can buy in the market, belongs to known technology, does not repeat them here.
Substrate 6 adopts a kind of harmless and Undec non-biocompatible thin film's material is prepared from oral cavity, such as: nitrocellulose, collagen, gelatin, chitosan, hyaluronate sodium, eggshell membrane, tetrafluoroethene or polylactic acid.
Three electrodes are that gold or carbon inactive, conductive material are prepared from, and three electrodes are respectively working electrode 2, reference electrode 3 and the 3rd electrode 1.As shown in Figure 2.
Contact conductor 5 and contact are made by silver, gold, carbon or silver-colored silver chloride material.Manufacture method is sputtering, evaporation or printing.
Sensitive material is formed by the solidification of enzyme, electron mediator and CNT.Electrode fixing sensitive material, such as enzyme, is to make sensor have specificity, only reacts with a kind of composition (such as lactic acid) in oral cavity or only detects concentration (such as oral cavity bacterium quantity) that is a kind of or a class material; Fixing nano-particle is the sensitivity in order to increase sensor, improves the sensitivity detected.Sensitive material the enzyme class of fixing have relation with the composition that will survey, such as: measure lactic acid and fix Lactate Oxidase, measure glucose fixing glucose oxidase.
Detection of Weak Signals module comprises central processing element 7, pin-saving chip 8, Signal Collection and amplify chip 9, communication chip 10, sensor contact interface 11 and peripheral circuit 12 thereof; Pin-saving chip 8, Signal Collection and amplify chip 9, communication chip 10, sensor contact interface 11 and peripheral circuit 12 thereof all communicate with central processing element 7;
Central processing element 7 stores the performing a programme of detection module, and performs corresponding instruction and action according to procedure order;
Pin-saving chip 8 is for storing the measurement result of the stricture of vagina tooth biosensor of detection module acquisition;
Signal Collection and amplify chip 9 for gather stricture of vagina tooth biosensor small-signal and by signal processing and amplifying;
Communication chip 10 is for realizing the connecting communication of Detection of Weak Signals module and host computer;
Sensor contact interface 11 is connected with the contact 4 of stricture of vagina tooth biosensor, provides voltage and measuring transducer output current to sensor, and wherein the concentration of the size sensor test substance of current output sensor is correlated with.
For the preparation method of stricture of vagina tooth biosensor arrangement that oral hygiene detects, have two kinds, the first adopts silk screen factor technology, and it two is utilize sputtering technology, specific as follows:
The technique of the stricture of vagina tooth biosensor one, utilizing screen printing technique to detect for the preparation of oral hygiene is as follows:
1) at the upper silver of non-biocompatible thin film's material (i.e. substrate 6) or silver-colored silver chloride slurry printed electrode contact 4, contact conductor 5 and three electrodes, and dried;
2) on working electrode 2 and reference electrode 3, carbon paste layer is printed, and dried;
3) at thin-film material (substrate 6) upper printing stricture of vagina tooth image, note making the contact 4 of 3 electrodes and sensor expose, can not be covered by stricture of vagina tooth image.Drying also solidifies stricture of vagina tooth image;
4) immobilized enzyme, electron mediator and CNT sensitive material on three electrodes exposed, and solidify, put 4 DEG C of Refrigerator stores.
5) prepare wireless communication module or data wire, conveniently stricture of vagina tooth biosensor is connected with Detection of Weak Signals module, carries out data communication.
The technique of the stricture of vagina tooth biosensor two, utilizing sputtering technology to detect for the preparation of oral hygiene is as follows:
1) on non-biocompatible thin film's material (i.e. substrate 6), make mask, mask covers the place not needing to make electrode, contact conductor and electrode contacts, and the place needing to make electrode, contact conductor and electrode contacts is exposed;
2) in sputtering stove, conveniently sputtering technology sputters thin film gold on the thin-film material being coated with mask, and thickness is about 100-200 dust;
3) tear mask, non-biocompatible thin film's material is formed electrode, contact conductor and electrode contacts 4;
4) at thin-film material (substrate 6) upper printing stricture of vagina tooth image, attention must make electrode and electrode contacts 4 expose, and can not be covered by stricture of vagina tooth image, and stricture of vagina tooth image can coated electrode lead-in wire 5.Drying also solidifies stricture of vagina tooth image;
5) immobilized enzyme, electron mediator and CNT sensitive material on three electrodes exposed, and solidify, put 4 DEG C of Refrigerator stores.
6) prepare wireless communication module or data wire, conveniently stricture of vagina tooth biosensor is connected with Detection of Weak Signals module, carries out data communication.
By reference to the accompanying drawings the specific embodiment of the present invention is described although above-mentioned; but not limiting the scope of the invention; one of ordinary skill in the art should be understood that; on the basis of technical scheme of the present invention, those skilled in the art do not need to pay various amendment or distortion that creative work can make still within protection scope of the present invention.
Claims (1)
1. an oral hygiene detects the detection method using stricture of vagina tooth biosensor arrangement, it is characterized in that, described stricture of vagina tooth biosensor arrangement, comprise stricture of vagina tooth biosensor, communication module and Detection of Weak Signals module, communication module is connected with Detection of Weak Signals module with the contact of stricture of vagina tooth biosensor respectively, obtain the output signal of stricture of vagina tooth biosensor, and by Signal transmissions to Detection of Weak Signals module; Described stricture of vagina tooth biosensor comprises sensitive material fixing on substrate, stricture of vagina tooth image, three electrodes combined with stricture of vagina tooth image and electrode, described three electrodes are fixed on the diverse location of the same face on substrate, three electrodes are connected with three contacts being positioned at substrate one edge independent of each other respectively by different contact conductors, that face of substrate that contact conductor is set is coated with stricture of vagina tooth image layer, and three electrodes and three contacts are exposed to outside stricture of vagina tooth image layer;
Described detection method is as follows:
1) the stricture of vagina tooth biosensor prepared carefully is attached on the front tooth of detected person;
2) in order to allow test substance in body before ensureing to measure) be in a metastable state, allow detected person loosen, arbitrarily walk about, normal secretions saliva;
3) after 20 minutes, the contact of Detection of Weak Signals module with stricture of vagina tooth biosensor is connected, measuring transducer signal, this signal is initial basic point signal;
4) for preventing exceptional value, measuring once every 10 minutes, measuring 3 times;
5) disconnect the connection of Detection of Weak Signals module and sensor contact, allow detected person's high strength run 5 kilometers fast;
6) then Detection of Weak Signals module is connected with sensor contact, measuring transducer signal, this signal be high-intensity exercise after test substance content in saliva;
7) for preventing exceptional value, measuring once every 10 minutes, measuring 3 times;
8) detected person is allowed to have a rest 2 hours, again Detection of Weak Signals module is connected with sensor contact, measuring transducer signal, this signal is a comparison signal, should be lower than test substance content after high-intensity exercise for test substance content after contrasting rest;
9) for preventing exceptional value, measuring once every 10 minutes, measuring 3 times.
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| CN1692278A (en) * | 2002-12-20 | 2005-11-02 | 松下电器产业株式会社 | Biosensor |
| JP2005192938A (en) * | 2004-01-09 | 2005-07-21 | Horiba Ltd | Method and system for analyzing intraoral health state |
| CN101339155A (en) * | 2007-07-04 | 2009-01-07 | 株式会社船井电机新应用技术研究所 | Enzyme electrode and enzyme sensor |
| WO2015112638A1 (en) * | 2014-01-21 | 2015-07-30 | The Regents Of The University Of California | Salivary biosensors and biofuel cells |
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