CN105176851A - Culture method for candida tropicalis rich in sterol - Google Patents
Culture method for candida tropicalis rich in sterol Download PDFInfo
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Abstract
The invention relates to a culture method for candida tropicalis rich in sterol. The method comprises the following concrete steps: preparing a culture medium with free aliphatic acid or fat as a sole carbon source; subjecting the prepared culture medium to high temperature sterilization, cooling the culture medium, inoculating candida tropicalis and then carrying out ventilation and aerobic fermentation; and after completion of fermentation, centrifugally collecting thallus so as to obtain the candida tropicalis rich in sterol. The content of sterol in obtained candida tropicalis cells can reach 6.23 to 7.12%, much higher compared with a conventional method; and the produced sterol mainly comprises sitosterol and campesterol, and sitosterol and campesterol have wider purposes compared with ergosterol.
Description
Technical field
The invention belongs to fermentation engineering field, be specifically related to a kind of cultural method being rich in the candida tropicalis of sterol.
Background technology
Sterol is the one in steroide, and the basic framework (being called perhydrocyclopentanophenanthrene core) of molecule is made up of three six-rings and a five-ring.C-3 position is connected with a hydroxyl, C-17 position is connected with the side chain be made up of 8 ~ 10 carbon atoms, and most sterol C-5 position is double bond.Because the side chain on C-17 position is different different with the material of hydroxy combining on C-3 position, the kind of sterol is also different.
Sterol is natural organic-compound in organism, is extensively present in the tissue of animal, plant, microorganism.Based on cholesterol (also known as cholesterol) in animal tissues; In plant tissue (mainly seed), mainly β-sitosterol, Stigmasterol and campesterol; Mainly ergosterol in microorganism.Therefore, cholesterol is called as zoosterol; β-sitosterol, Stigmasterol and campesterol are called as plant sterol; Ergosterol is called as microorganism sterol.Plant sterol is mainly derived from vegetables oil, and its content is only 0.04% ~ 0.1%.Due to the moiety that sterol is eukaryotic cell membrane, therefore, all eukaryotes are all containing sterol, but the kind of sterol and content thereof have very large difference, wherein the sterol of yeast saccharomyces cerevisiae comparatively horn of plenty in Saccharomycodes.Yeast saccharomyces cerevisiae can synthesize about 20 kinds of sterols, and sterol total content is 4.6% (dry cell weight) to the maximum, and wherein ergosterol accounts for 90% of total sterol.
Sterol is described as " key of life ", has very important physiological function.β-sitosterol, Stigmasterol and campesterol have serum cholesterol-lowering, reducing blood-fat, antitumor, reduce heart trouble incidence, prevent prostatosis, and anticancer, regulate the growth of animal, anti-inflammatory, antiviral, the effect such as delay senility.β-sitosterol, Stigmasterol, campesterol and ergosterol are also the precursors of multiple hormone, vitamins D and steroide synthesis.Therefore, sterol is widely used in the industrial circles such as food, medicine, makeup.
Application number be 95114213.5 Chinese patent disclose a kind of manufacture method being rich in ergosterin yeast, be carbon source with molasses in substratum, nutrient media components also comprises appropriate urea, ammonium sulfate, phosphoric acid etc. and carrys out fermentation culture yeast, and in the yeast cell of gained, Quantitative Determination of Ergosterol can reach 1.4% ~ 1.6%.Application number be 201080055130.5 Chinese patent disclose a kind of manufacture method of the sterol by the non-yeast of yeast production, the yeast used can be yeast saccharomyces cerevisiae (Saccharomycescerevisiae), pichia spp (Pichiaspp), kluyveromyces (Klyuveromycesspp), debaryomyces hansenii (Hansenulaspp), fission yeast (Schizosaccharomycesspec.) conciliates fat yeast (Yarrowialipolytica), the sterol produced is 7-DHC, 25-hydroxyl-7-DHC and 25-hydroxyl ergosterol.Be that carbon source through fermentation is cultivated the method being rich in the candida tropicalis of sterol and is at home and abroad not reported in document with lipid acid.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of candida tropicalis cultural method being rich in sterol, the Candida tropicalis cells gathered in the crops is rich in sterol, its sterol content can reach 6.23% ~ 7.12%, and the sterol produced is based on Sitosterol and campesterol.
Principle of the present invention: candida tropicalis has and can carry out the characteristic of growth and breeding using free fatty acids and grease as carbon source.The substratum that preparation is sole carbon source with free fatty acids or fat; Substratum is after high-temperature sterilization, cooling, and inoculation candida tropicalis carries out ventilation aerobic fermentation; After fermentation ends, collected by centrifugation thalline, namely obtains the Candida tropicalis cells being rich in sterol.
For solving the problems of the technologies described above, the present invention is implemented by the following technical solutions:
Be rich in a cultural method for the candida tropicalis of sterol, comprise the following steps:
The preparation of a, seed culture medium and sterilizing: by proportioning in 1L water, add 20 ~ 30g glucose, 10 ~ 20g peptone, 5 ~ 10g yeast powder, according to described proportioning, add in triangular flask after various composition is weighed, stirred at ambient temperature is even, obtained seed culture medium; Put into high-pressure sterilizing pot with after 8 layers of gauze sealing, in 115 DEG C ~ 121 DEG C sterilizing 15 ~ 20min, after sterilizing, triangular flask is cooled to room temperature;
The preparation of b, seed liquor: slant preservation bacterial classification 2 ~ 3 ring of picking candida tropicalis, is inoculated in the seed culture medium described in step a, in 30 DEG C, 180 ~ 200rpm shaking table shaking culture 48h, for subsequent use;
The preparation of c, fermention medium and sterilizing: in 1L water, add 40 ~ 50g free fatty acids or grease (described grease is soybean oil, peanut oil, rapeseed oil, cottonseed wet goods), 1.0 ~ 2.0g yeast powder, 0.1 ~ 0.2g magnesium sulfate, 0.1 ~ 0.2g dipotassium hydrogen phosphate, 0.1 ~ 0.2g potassium primary phosphate; According to described proportioning, add in mechanical agitation type fermentor tank after being weighed by various composition, stirred at ambient temperature is even, and by 0.5M sodium hydroxide solution adjust ph 6.8 ~ 7.0, obtains fermention medium; Then pass into steam to fermentor tank and start sterilizing, treat that tank temperature rises to 115 DEG C ~ 121 DEG C, insulation 30min, after sterilizing terminates, introduce water coolant and fermentation medium temperature is down to 25 DEG C ~ 30 DEG C;
D, fermentation: Candida tropicalis seed liquor described in step b is inoculated among the substratum in fermentor tank described in step c with 8% ~ 10% inoculum size, control leavening temperature 28 DEG C ~ 30 DEG C, mechanical stirring rotating speed 220rpm ~ 240rpm, air flow 1.0 ~ 1.2vvm, pH value 6.8 ~ 7.0, fermentation 96 ~ 108h.
E, centrifugal, washing: the fermented liquid described in steps d is positioned in whizzer, centrifugal 15 ~ the 20min of 5000 ~ 5500rpm, abandon the remaining free fatty acids in upper strata or grease and clear liquid, the bacterium mud (wet thallus) of collecting precipitation bottom whizzer, add 50 DEG C ~ 60 DEG C deionized waters according to the ratio of 1:50 ~ 1:80 and make bacteria suspension, stir 5 ~ 8min, and then carry out centrifugal, washing like this 3 times, obtains candida tropicalis wet thallus;
F, drying: the candida tropicalis wet thallus described in step e is carried out 40 DEG C ~ 50 DEG C cold air dryings, namely obtain the candida tropicalis dry mycelium being rich in sterol.
In described step f, its total sterol content is 6.23% ~ 7.12%.Wherein Sitosterol accounts for 53.4% of total sterol; Campesterol accounts for 37.9% of total sterol.
Preferably, in described step a, the material of seed culture medium consists of: 20g/L glucose, 20g/L peptone and 10g/L yeast powder.
Preferably, the consisting of of fermention medium in described step c: in 1L water, add 50g soybean oil, 2.0g yeast powder, 0.1g magnesium sulfate, 0.1g dipotassium hydrogen phosphate, 0.1g potassium primary phosphate.
Preferably, in described step c, grease is the one in soybean oil, peanut oil, rapeseed oil, Oleum Gossypii semen.
Preferably, a kind of cultural method being rich in the candida tropicalis of sterol, comprises following concrete steps:
The preparation of a, seed culture medium and sterilizing: in 1L water, add 30g glucose, 10g peptone, 5g yeast powder, according to described proportioning, adds in triangular flask after being weighed by various composition, and stirred at ambient temperature is even, obtained seed culture medium; Put into high-pressure sterilizing pot with after 8 layers of gauze sealing, 121 DEG C of sterilizing 20min, are cooled to room temperature triangular flask after sterilizing;
The preparation of b, seed liquor: slant preservation bacterial classification 2 ~ 3 ring of picking candida tropicalis, is inoculated in the seed culture medium described in step a, 30 DEG C, 200rpm shaking table shaking culture 48h;
The preparation of c, fermention medium and sterilizing: in 1L water, add 50g soybean oil, 2.0g yeast powder, 0.1g magnesium sulfate, 0.1g dipotassium hydrogen phosphate, 0.1g potassium primary phosphate, according to described proportioning, add after various composition is weighed in mechanical agitation type fermentor tank, stirred at ambient temperature is even, and by 0.5M sodium hydroxide solution adjust ph 7.0, obtain fermention medium; Then pass into steam to fermentor tank and start sterilizing, treat that tank temperature rises to 121 DEG C, insulation 30min, after sterilizing terminates, introduce water coolant and fermentation medium temperature is down to 25 DEG C ~ 30 DEG C;
D, fermentation: Candida tropicalis seed liquor described in step b be inoculated among the substratum in fermentor tank described in step c with 10% inoculum size, control leavening temperature 30 DEG C, mechanical stirring rotating speed 240rpm, air flow 1.2vvm, pH value 7.0, fermentation 108h;
E, centrifugal, washing: the fermented liquid described in steps d is positioned in whizzer, the centrifugal 20min of 5500rpm, abandons the remaining soybean oil in upper strata and clear liquid.The bacterium mud (wet thallus) of collecting precipitation bottom whizzer, adds 60 DEG C of deionized waters according to the ratio of 1:80 and makes bacteria suspension, stirs 5 ~ 8min, and then carries out centrifugal, and so washing 3 times, obtains candida tropicalis wet thallus;
F, drying: the candida tropicalis wet thallus described in step e is carried out 40 DEG C ~ 50 DEG C cold air dryings, namely obtain the candida tropicalis dry mycelium being rich in sterol.
In described step f, its total sterol content is 6.95%, and in produced total sterol, Sitosterol accounts for 53.4%, and campesterol accounts for 37.9%.
The beneficial effect that the present invention compared with prior art has is:
1. obtained Candida tropicalis cells sterol content reaches 6.23% ~ 7.12%, far away higher than general
Sterol content (1.4% ~ 4.6%) in the yeast cell that circulation method is cultivated.
2. the sterol that obtained Candida tropicalis cells produces is based on Sitosterol and campesterol, and the sterol in the yeast cell that usual way is cultivated is based on ergosterol.Compare with ergosterol, Sitosterol and campesterol purposes more extensive.
3. fermentable is adopted to produce plant sterol in a large number: Sitosterol and campesterol.
Embodiment
Below in conjunction with embodiment, the present invention is further illustrated.
Embodiment one
Be rich in the candida tropicalis cultural method of sterol: take oleic acid as sole carbon source.
The preparation of a, seed culture medium and sterilizing: in 1L water, add 30g glucose, 10g peptone, 10g yeast powder.According to described proportioning, add in triangular flask after being weighed by various composition, stirred at ambient temperature is even, obtained seed culture medium; High-pressure sterilizing pot is put into, 115 DEG C of sterilizing 15min with after 8 layers of gauze sealing.After sterilizing, triangular flask is cooled to room temperature.
The preparation of b, seed liquor: slant preservation bacterial classification 2 ~ 3 ring of picking candida tropicalis, is inoculated in the seed culture medium described in step a, 30 DEG C, 200rpm shaking table shaking culture 48h.
The preparation of c, fermention medium and sterilizing: in 1L water, add 40g oleic acid, 2.0g yeast powder, 0.1g magnesium sulfate, 0.1g dipotassium hydrogen phosphate, 0.1g potassium primary phosphate.According to described proportioning, add in mechanical agitation type fermentor tank after being weighed by various composition, stirred at ambient temperature is even, and by 0.5M sodium hydroxide solution adjust ph 7.0, obtains fermention medium; Then pass into steam to fermentor tank and start sterilizing, treat that tank temperature rises to 121 DEG C, insulation 30min, after sterilizing terminates, introduce water coolant and fermentation medium temperature is down to 25 DEG C ~ 30 DEG C.
D, fermentation: Candida tropicalis seed liquor described in step b be inoculated among the substratum in fermentor tank described in step c with 10% inoculum size, control leavening temperature 30 DEG C, mechanical stirring rotating speed 220rpm, air flow 1.2vvm, pH value 7.0, fermentation 108h.
E, centrifugal, washing: the fermented liquid described in steps d is positioned in whizzer, the centrifugal 20min of 5000rpm, abandons the remaining oleic acid in upper strata and clear liquid.The bacterium mud (wet thallus) of collecting precipitation bottom whizzer, adds 50 DEG C of deionized waters according to the ratio of 1:50 and makes bacteria suspension, stirs 5 ~ 8min, and then carries out centrifugal, so washing 3 times, final obtains candida tropicalis wet thallus.
F, drying: the candida tropicalis wet thallus described in step e is carried out 40 DEG C ~ 50 DEG C cold air dryings, namely obtain the candida tropicalis dry mycelium being rich in sterol, its sterol content is 7.12%.The sterol that the candida tropicalis obtained produces is based on Sitosterol (56.7%) and campesterol (38.2%).
Embodiment two
Be rich in the candida tropicalis cultural method of sterol: take linolic acid as sole carbon source.
The preparation of a, seed culture medium and sterilizing: in 1L water, add 20g glucose, 20g peptone, 10g yeast powder.According to described proportioning, add in triangular flask after being weighed by various composition, stirred at ambient temperature is even, obtained seed culture medium; High-pressure sterilizing pot is put into, 115 DEG C of sterilizing 15min with after 8 layers of gauze sealing.After sterilizing, triangular flask is cooled to room temperature.
The preparation of b, seed liquor: slant preservation bacterial classification 2 ~ 3 ring of picking candida tropicalis, is inoculated in the seed culture medium described in step a, 30 DEG C, 180rpm shaking table shaking culture 48h.
The preparation of c, fermention medium and sterilizing: in 1L water, add 40g linolic acid, 2.0g yeast powder, 0.1g magnesium sulfate, 0.2g dipotassium hydrogen phosphate, 0.2g potassium primary phosphate.According to described proportioning, add in mechanical agitation type fermentor tank after being weighed by various composition, stirred at ambient temperature is even, and by 0.5M sodium hydroxide solution adjust ph 6.8, obtains fermention medium; Then pass into steam to fermentor tank and start sterilizing, treat that tank temperature rises to 121 DEG C, insulation 30min, after sterilizing terminates, introduce water coolant and fermentation medium temperature is down to 25 DEG C ~ 30 DEG C.
D, fermentation: Candida tropicalis seed liquor described in step b be inoculated among the substratum in fermentor tank described in step c with 8% inoculum size, control leavening temperature 30 DEG C, mechanical stirring rotating speed 220rpm, air flow 1.0vvm, pH value 7.0, fermentation 96h.
E, centrifugal, washing: the fermented liquid described in steps d is positioned in whizzer, the centrifugal 20min of 5500rpm, abandons the remaining linolic acid in upper strata and clear liquid.The bacterium mud (wet thallus) of collecting precipitation bottom whizzer, adds 50 DEG C of deionized waters according to the ratio of 1:50 and makes bacteria suspension, stirs 5 ~ 8min, and then carries out centrifugal, so washing 3 times, final obtains candida tropicalis wet thallus.
F, drying: the candida tropicalis wet thallus described in step e is carried out 40 DEG C ~ 50 DEG C cold air dryings, namely obtain the candida tropicalis dry mycelium being rich in sterol, its sterol content is 6.23%.The sterol that the candida tropicalis obtained produces is based on Sitosterol (50.1%) and campesterol (40.3%).
Embodiment three
Be rich in the candida tropicalis cultural method of sterol: take soybean oil as sole carbon source.
The preparation of a, seed culture medium and sterilizing: in 1L water, add 30g glucose, 10g peptone, 5g yeast powder.According to described proportioning, add in triangular flask after being weighed by various composition, stirred at ambient temperature is even, obtained seed culture medium; High-pressure sterilizing pot is put into, 121 DEG C of sterilizing 20min with after 8 layers of gauze sealing.After sterilizing, triangular flask is cooled to room temperature.
The preparation of b, seed liquor: slant preservation bacterial classification 2 ~ 3 ring of picking candida tropicalis, is inoculated in the seed culture medium described in step a, 30 DEG C, 200rpm shaking table shaking culture 48h.
The preparation of c, fermention medium and sterilizing: in 1L water, add 50g soybean oil, 2.0g yeast powder, 0.1g magnesium sulfate, 0.1g dipotassium hydrogen phosphate, 0.1g potassium primary phosphate.According to described proportioning, add in mechanical agitation type fermentor tank after being weighed by various composition, stirred at ambient temperature is even, and by 0.5M sodium hydroxide solution adjust ph 7.0, obtains fermention medium; Then pass into steam to fermentor tank and start sterilizing, treat that tank temperature rises to 121 DEG C, insulation 30min, after sterilizing terminates, introduce water coolant and fermentation medium temperature is down to 25 DEG C ~ 30 DEG C.
D, fermentation: Candida tropicalis seed liquor described in step b be inoculated among the substratum in fermentor tank described in step c with 10% inoculum size, control leavening temperature 30 DEG C, mechanical stirring rotating speed 240rpm, air flow 1.2vvm, pH value 7.0, fermentation 108h.
E, centrifugal, washing: the fermented liquid described in steps d is positioned in whizzer, the centrifugal 20min of 5500rpm, abandons the remaining soybean oil in upper strata and clear liquid.The bacterium mud (wet thallus) of collecting precipitation bottom whizzer, adds 60 DEG C of deionized waters according to the ratio of 1:80 and makes bacteria suspension, stirs 5 ~ 8min, and then carries out centrifugal, so washing 3 times, final obtains candida tropicalis wet thallus.
F, drying: the candida tropicalis wet thallus described in step e is carried out 40 DEG C ~ 50 DEG C cold air dryings, namely obtain the candida tropicalis dry mycelium being rich in sterol, its sterol content is 6.95%.The sterol that the candida tropicalis obtained produces is based on Sitosterol (53.4%) and campesterol (37.9%).
Claims (8)
1. be rich in a cultural method for the candida tropicalis of sterol, it is characterized in that, comprise the following steps:
The preparation of a, seed culture medium and sterilizing: by proportioning in 1L water, add 20 ~ 30g glucose, 10 ~ 20g peptone, 5 ~ 10g yeast powder, according to described proportioning, add in triangular flask after various composition is weighed, stirred at ambient temperature is even, obtained seed culture medium; Put into high-pressure sterilizing pot with after 8 layers of gauze sealing, in 115 DEG C ~ 121 DEG C sterilizing 15 ~ 20min, after sterilizing, triangular flask is cooled to room temperature;
The preparation of b, seed liquor: slant preservation bacterial classification 2 ~ 3 ring of picking candida tropicalis, is inoculated in the seed culture medium described in step a, in 30 DEG C, 180 ~ 200rpm shaking table shaking culture 48h, for subsequent use;
The preparation of c, fermention medium and sterilizing: in 1L water, add 40 ~ 50g free fatty acids or grease (described grease is soybean oil, peanut oil, rapeseed oil, cottonseed wet goods), 1.0 ~ 2.0g yeast powder, 0.1 ~ 0.2g magnesium sulfate, 0.1 ~ 0.2g dipotassium hydrogen phosphate, 0.1 ~ 0.2g potassium primary phosphate; According to described proportioning, add in mechanical agitation type fermentor tank after being weighed by various composition, stirred at ambient temperature is even, and by 0.5M sodium hydroxide solution adjust ph 6.8 ~ 7.0, obtains fermention medium; Then pass into steam to fermentor tank and start sterilizing, treat that tank temperature rises to 115 DEG C ~ 121 DEG C, insulation 30min, after sterilizing terminates, introduce water coolant and fermentation medium temperature is down to 25 DEG C ~ 30 DEG C;
D, fermentation: Candida tropicalis seed liquor described in step b is inoculated among the substratum in fermentor tank described in step c with 8% ~ 10% inoculum size, control leavening temperature 28 DEG C ~ 30 DEG C, mechanical stirring rotating speed 220rpm ~ 240rpm, air flow 1.0 ~ 1.2vvm, pH value 6.8 ~ 7.0, fermentation 96 ~ 108h.
E, centrifugal, washing: the fermented liquid described in steps d is positioned in whizzer, centrifugal 15 ~ the 20min of 5000 ~ 5500rpm, abandon the remaining free fatty acids in upper strata or grease and clear liquid, the bacterium mud (wet thallus) of collecting precipitation bottom whizzer, add 50 DEG C ~ 60 DEG C deionized waters according to the ratio of 1:50 ~ 1:80 and make bacteria suspension, stir 5 ~ 8min, and then carry out centrifugal, washing like this 3 times, obtains candida tropicalis wet thallus;
F, drying: the candida tropicalis wet thallus described in step e is carried out 40 DEG C ~ 50 DEG C cold air dryings, namely obtain the candida tropicalis dry mycelium being rich in sterol.
2. a kind of cultural method being rich in the candida tropicalis of sterol as claimed in claim 1, it is characterized in that: in described step f, its total sterol content is 6.23% ~ 7.12%.
3. a kind of cultural method being rich in the candida tropicalis of sterol as claimed in claim 2, is characterized in that: wherein Sitosterol accounts for 53.4% of total sterol; Campesterol accounts for 37.9% of total sterol.
4. a kind of cultural method being rich in the candida tropicalis of sterol as claimed in claim 1, is characterized in that: in described step a, the material of seed culture medium consists of: 20g/L glucose, 20g/L peptone and 10g/L yeast powder.
5. a kind of cultural method being rich in the candida tropicalis of sterol as claimed in claim 1, is characterized in that:
The consisting of of fermention medium in described step c: in 1L water, add 50g soybean oil, 2.0g yeast powder, 0.1g magnesium sulfate, 0.1g dipotassium hydrogen phosphate, 0.1g potassium primary phosphate.
6. a kind of cultural method being rich in the candida tropicalis of sterol as claimed in claim 1, is characterized in that:
In described step c, grease is the one in soybean oil, peanut oil, rapeseed oil, Oleum Gossypii semen.
7. a kind of cultural method being rich in the candida tropicalis of sterol as claimed in claim 1, is characterized in that: comprise following concrete steps:
The preparation of a, seed culture medium and sterilizing: in 1L water, add 30g glucose, 10g peptone, 5g yeast powder, according to described proportioning, adds in triangular flask after being weighed by various composition, and stirred at ambient temperature is even, obtained seed culture medium; Put into high-pressure sterilizing pot with after 8 layers of gauze sealing, 121 DEG C of sterilizing 20min, are cooled to room temperature triangular flask after sterilizing;
The preparation of b, seed liquor: slant preservation bacterial classification 2 ~ 3 ring of picking candida tropicalis, is inoculated in the seed culture medium described in step a, 30 DEG C, 200rpm shaking table shaking culture 48h;
The preparation of c, fermention medium and sterilizing: in 1L water, add 50g soybean oil, 2.0g yeast powder, 0.1g magnesium sulfate, 0.1g dipotassium hydrogen phosphate, 0.1g potassium primary phosphate, according to described proportioning, add after various composition is weighed in mechanical agitation type fermentor tank, stirred at ambient temperature is even, and by 0.5M sodium hydroxide solution adjust ph 7.0, obtain fermention medium; Then pass into steam to fermentor tank and start sterilizing, treat that tank temperature rises to 121 DEG C, insulation 30min, after sterilizing terminates, introduce water coolant and fermentation medium temperature is down to 25 DEG C ~ 30 DEG C;
D, fermentation: Candida tropicalis seed liquor described in step b be inoculated among the substratum in fermentor tank described in step c with 10% inoculum size, control leavening temperature 30 DEG C, mechanical stirring rotating speed 240rpm, air flow 1.2vvm, pH value 7.0, fermentation 108h;
E, centrifugal, washing: the fermented liquid described in steps d is positioned in whizzer, the centrifugal 20min of 5500rpm, abandons the remaining soybean oil in upper strata and clear liquid.The bacterium mud (wet thallus) of collecting precipitation bottom whizzer, adds 60 DEG C of deionized waters according to the ratio of 1:80 and makes bacteria suspension, stirs 5 ~ 8min, and then carries out centrifugal, and so washing 3 times, obtains candida tropicalis wet thallus;
F, drying: the candida tropicalis wet thallus described in step e is carried out 40 DEG C ~ 50 DEG C cold air dryings, namely obtain the candida tropicalis dry mycelium being rich in sterol.
8. a kind of cultural method being rich in the candida tropicalis of sterol as claimed in claim 7, it is characterized in that: in described step f, its total sterol content is 6.95%, and in produced total sterol, Sitosterol accounts for 53.4%, and campesterol accounts for 37.9%.
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102352400A (en) * | 2011-10-14 | 2012-02-15 | 河北科技大学 | Method for producing phytosterol from deodorized distillate of vegetable fat obtained by microbial fermentation |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102352400A (en) * | 2011-10-14 | 2012-02-15 | 河北科技大学 | Method for producing phytosterol from deodorized distillate of vegetable fat obtained by microbial fermentation |
Non-Patent Citations (3)
| Title |
|---|
| GUOQUN ZHAO ET AL.: "Fermentation of soybean oil deodorizer distillate with Candida tropicalis to concentrate phytosterols and to produce sterols-rich yeast cells", 《JOURNAL OF INDUSTRIAL MICROBIOLOGY&BIOTECHNOLOGY》 * |
| MEINRAD BOLL ET AL.: "Effect of unsaturated fatty acids on sterol biosynthesis in yeast.", 《BIOCHIMICA ET BIOPHYSICA ACTA(BBA)-LIPIDS AND LIPID METABOLISM》 * |
| 李洁莉等: "猴头菌醇提浸膏和水提浸膏甾醇类化合物的比较研究", 《中国中药杂志》 * |
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