CN105175561B - A kind of preparation method of anti-oxidant straw mushroom polysaccharide - Google Patents
A kind of preparation method of anti-oxidant straw mushroom polysaccharide Download PDFInfo
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Abstract
The invention discloses a kind of preparation method of anti-oxidant straw mushroom polysaccharide, this method includes straw mushroom pretreatment, crushing, Microwave Extraction, centrifugation or filtering, concentration, alcohol precipitation separation, desalination, is dried to obtain anti-oxidant straw mushroom polysaccharide.The multistage microwave amplifier alcohol precipitation straw mushroom polysaccharide process of the present invention, Polyose extraction efficiency high, antioxidation activity is strong, easily operated, and equipment investment is few, and process route is simple, is adapted to industrialized production；By the extraction of anti-oxidant straw mushroom polysaccharide, solve straw mushroom and be not easy fresh-retaining preserving, extension industry chain, expand the straw mushroom market demand.
Description
Technical field
The invention belongs to the technical field of agricultural byproducts intensive processing, more particularly to a kind of preparation of anti-oxidant straw mushroom polysaccharide
Method.
Background technology
Straw mushroom has good health value, invigorates the spleen and benefits qi, clearing away summerheat, improves immunity, accelerating wound healing etc.
Effect, it also has the reduction effect such as cholesterol and anticancer, is the nutritional health food of excellent food medicine dual-purpose type.
To the oxidation-resistant active ingredient in straw mushroom, there are reports.The researchs such as Li Le have shown that the thick of the first alcohol and water of straw mushroom
Extract has the function that radicals scavenging and suppresses Rat Erythrocytes haemolysis.Sun Yanfang etc. is using microwave radiation exaraction to straw mushroom
Polyphenol content and antioxidation activity are studied, the results showed that straw mushroom polyphenol is respectively provided with very strong oxidation resistance.Cheung etc.
The methanolic extract of straw mushroom and the antioxidation activity of water extract are determined using 3 kinds of distinct methods, is found total in water extract
Phenol content is higher than methanolic extract, and its antioxidation activity is also above methanolic extract.Zhao Junxia etc. is with different organic solvents to grass
Metabolite in mushroom nutrient solution and mycelium carries out separation and Extraction, and metabolism extract has higher DPPH clearance rates, i.e.,
Higher antioxidation activity, its active ingredient contain thick triterpene and Flavonoid substances.Polysaccharide be pharmacology important in edible mushroom into
/ mono-.Research in recent years shows that polysaccharide also has in addition to the function such as anti-aging, antiviral, anticancer, immunological regulation
Wheat Protein.The research of straw mushroom polysaccharide anti-oxidative activity yet there are no report.
Microwave auxiliary extraction technology is a kind of method that new development in recent years is got up, with traditional extracting method as backflow carries
Take, organic solvent extraction the methods of compare, microwave extraction technology have the advantages that it is easy to operate, rapidly and efficiently, energy-conservation, it is time saving.
Straw mushroom is the mushroom class that fresh-retaining preserving is most not easy in edible mushroom, the afterripening after adopting strongly, and picking time
Temperature is higher, can not store at a lower temperature again, and these factors constrain the circulation and large-scale production of fresh straw mushroom.
With the raising of Volvaria volvacea cultivation technology and the quick increase of yield, strengthen developing the deep processed product of series, change
Become single product structure, extension industry chain, meet the different consumer groups, expand the demand in market.
The content of the invention
Goal of the invention：It is an object of the invention to provide a kind of preparation method of anti-oxidant straw mushroom polysaccharide.
Technical scheme：A kind of preparation method of anti-oxidant straw mushroom polysaccharide provided by the invention, this method are located in advance including straw mushroom
Reason, crushing, Microwave Extraction, centrifugation or filtering, concentration, alcohol precipitation separation, desalination, it is dried to obtain anti-oxidant straw mushroom polysaccharide.
This method comprises the following steps：
1) straw mushroom pre-processes：The straw mushroom of non-self-dissolving is selected, clean, impurity elimination, section, 105 DEG C of inactivation 10-15min；
2) crush：Pretreatment straw mushroom in step 1) is added into agitator, adds the water of 5-10 times of straw mushroom weight, it is fully even
Slurry；
3) multistage microwave amplifier；
4) centrifuge or filter：Material in step 3) is cooled to 20-50 DEG C, centrifugal rotational speed 3000-4000r/min, the time
For 5-10min, or filtering, take clear liquid standby；
5) concentrate：Clear liquid described in step 4) is concentrated, ultrafiltration, normal pressure can be used or concentration is evaporated under reduced pressure, make to carry
Take liquid to be concentrated into original volume 1/5-1/10, be cooled to room temperature, there is precipitation to be centrifuged, rotating speed 3000-4000r/min, from
Heart time 5-10min, abandons precipitation, obtains straw mushroom polysaccharide and slightly carries concentrate；
6) alcohol precipitation separates：In concentrate, 95% ethanol will be slowly added into described in step 5) to concentration of alcohol up to 30%,
Adding in ethanol process to be stirred continuously, after staticly settling 6-24 hours, siphon supernatant to original volume 1/5-1/10, and bottom liquid
Body is centrifuged, rotating speed 3500-4000r/min, centrifugation time 10-15min, will continue to add in the supernatant after centrifugation
Enter 95% ethanol, to concentration of alcohol up to 80%, stand overnight, centrifuge, obtain two parts precipitation；
7) desalination：Add a small amount of water fully to dissolve the precipitation described in step 6), carry out ultrafiltration or dialysis, molecule interception are
5-10kD, obtain trapped fluid；
8) dry：By the precipitation drying described in step 6), 50-70 DEG C of oven for drying；Or by the trapped fluid described in step 7)
Freeze-drying or 50-60 DEG C of vacuum drying chamber drying, dried object crush, and water content is less than 4%, produces anti-oxidant straw mushroom polysaccharide.
Step 3) the Microwave Extraction parameter is：The water of 20-25 times of straw mushroom weight is added in straw mushroom homogenate, in normal temperature and pressure
Lower carry out Microwave Extraction, microwave power 210-490w, Microwave Extraction total time are 12-18min, interval 1 time, the intermittent time
10-20min, obtained material is standby.
Being concentrated under reduced pressure in the step 5), vacuum 0.07-0.095MPa, temperature are 60-80 DEG C；Atmospheric evaporation is dense
Contracting temperature is 90-100 DEG C.
Ultrafiltration described in the step 5) and step 7), ultrafiltration membrane molecule interception 5kD, at 25 DEG C, inlet pressure 2~
5kg/m2, gateway pressure difference do not exceed 1.5kg/m2。
Beneficial effect：Compare and prior art, the present invention have advantages below：
1) multistage microwave amplifier alcohol precipitation straw mushroom polysaccharide process, Polyose extraction efficiency high, antioxidation activity is strong, easily operated, if
Standby less investment, process route is simple, is adapted to industrialized production；
2) by the extraction of anti-oxidant straw mushroom polysaccharide, solve straw mushroom and be not easy fresh-retaining preserving, extension industry chain, expand grass
The mushroom market demand；
3) active strong, the extraction efficiency height of the Microwave Extraction straw mushroom polysaccharide anti-oxidative that the present invention designs.Microwave Extraction straw mushroom is more
Sugared extraction time control substantially reduces extraction time in 1h.Microwave Extraction straw mushroom polysaccharide yield is 15.56%, slightly above
Hot water carries polysaccharide 15.00%.Microwave Extraction straw mushroom polysaccharide has stronger scavenging action and reducing power to OH and DPPH,
Polysaccharide is carried higher than hot water.In the range of 0.1~5mg/mL of mass concentration, multistage microwave amplifier straw mushroom polysaccharide to OH, DPPH most
Major clean-up operation rate is respectively 100%, 76.15%.Microwave Extraction straw mushroom polysaccharide has stronger reducing power, reflects that its is anti-oxidant
Ability is stronger；
4) the multistage microwave amplifier straw mushroom polysaccharide optimize technique antioxidation activity that designs of the present invention is strong, recovery rate is high.Microwave carries
The parameter is taken to be：The water of 20-25 times of straw mushroom weight is added in straw mushroom homogenate, carries out Microwave Extraction, microwave power at normal temperatures and pressures
For 210-350w, Microwave Extraction total time is 15-18min, interval 1 time, intermittent time 10-20min.Straw mushroom polysaccharide extract rate is
15.01-15.56%, when polysaccharide concentration is 2.5mg/mL, multistage microwave amplifier straw mushroom polysaccharide is 56.42- to DPPH clearance rates
75.67%；
5) the ethanol precipitation separation straw mushroom polysaccharide process equipment investment that the present invention designs is few, and process route is simple, easily
In operation, it is adapted to large-scale production.Oh resistant ability can be strengthened using ethanol precipitation heat of dissociation water extraction straw mushroom polysaccharide,
And there are stronger DPPH scavenging capacities.80% classification alcohol precipitation straw mushroom polysaccharide is higher than to OH scavenging capacities is not classified alcohol precipitation straw mushroom
Polysaccharide, it is 71.77-72.26% to OH clearance rates when polysaccharide concentration is 2.5mg/mL.30% classification alcohol precipitation straw mushroom polysaccharide
Stronger to DPPH scavenging capacities, when polysaccharide concentration is 2.5mg/mL, clearance rate is 73.36%.
Brief description of the drawings
Fig. 1 present invention extraction straw mushroom polysaccharide compares OH scavenging capacity；
Fig. 2 present invention extraction straw mushroom polysaccharide compares DPPH scavenging capacity；
The comparison of Fig. 3 present invention extraction straw mushroom polysaccharide reducing powers；
Fig. 4 alcohol precipitation straw mushroom polysaccharide oh resistant activity；
The anti-DPPH activity of Fig. 5 alcohol precipitation straw mushroom polysaccharide；
Fig. 6 straw mushroom polysaccharide ethanol precipitation process route charts.
Embodiment
According to following embodiments, the present invention may be better understood.It is however, as it will be easily appreciated by one skilled in the art that real
Apply specific material proportion, process conditions and its result described by example and be merely to illustrate the present invention, without that will not also should limit
The present invention described in detail in claims processed.
Embodiment 1
1) pre-process：The straw mushroom of the non-parachute-opening of selection, clean, impurity elimination, section, 105 DEG C of inactivation 10min.
2) crush：Pretreatment straw mushroom in step 1) is added into agitator, adds the water of 10 times of straw mushroom weight, fully homogenate,
There can not be block.
3) multistage microwave amplifier：Straw mushroom homogenate in step 2) is added to the water of 20 times of straw mushroom weight, carried out at normal temperatures and pressures
Microwave Extraction, microwave power 210w, Microwave Extraction total time are 18min, interval 1 time, intermittent time 10min, by what is obtained
Material is standby.
4) centrifuge：Material in step 3) is cooled to 30 DEG C, centrifugal rotational speed 4000r/min, time 10min, takes clear liquid
It is standby.
5) concentrate：Filtered fluid described in step 4) is concentrated, using ultrafiltration concentration, extract solution is concentrated into original volume
1/10.Ultrafiltration membrane molecule interception 5kD, at 25 DEG C, 2~5kg/m of inlet pressure2, gateway pressure difference do not exceed 1.5kg/m2,
Obtain concentrate.
6) alcohol precipitation separates：In concentrate, 95% ethanol will be slowly added into described in step 5) to concentration of alcohol up to 30%,
Adding in ethanol process to be stirred continuously, and after staticly settling 24 hours, siphon supernatant to original volume 1/5, lower liquid is carried out
Centrifuge, rotating speed 4000r/min, centrifugation time 10min, 95% ethanol will be continuously added in the supernatant after centrifugation, extremely
Concentration of alcohol stands overnight, centrifuged, obtain two parts precipitation up to 80%.
7) dry：In the precipitation drying described in step 6), 60 DEG C of oven for drying, crush, water content is less than 4%, produces anti-
Aoxidize straw mushroom polysaccharide.
8) multistage microwave amplifier straw mushroom polysaccharide has stronger scavenging action and reducing power to OH and DPPH, is above heat
The straw mushroom polysaccharide of water extraction method extraction.Oh resistant ability, Yi Jiyou can be strengthened using ethanol precipitation separation straw mushroom polysaccharide
Stronger DPPH scavenging capacities.
The influence of different time to straw mushroom polysaccharide extract rate under 210w microwave powers of table 1
| Time (min) | 15 | 18 | 21 | 24 | 27 |
| Recovery rate (%) | 12.74 | 15.45 | 14.52 | 10.36 | 6.11 |
| Clearance rate (%) | 58.11 | 56.42 | 54.23 | 52.12 | 50.23 |
Embodiment 2
1) pre-process：The straw mushroom of the non-parachute-opening of selection, clean, impurity elimination, section, 105 DEG C of inactivation 15min.
2) crush：Pretreatment straw mushroom in step 1) is added into agitator, adds the water of 8 times of straw mushroom weight, fully homogenate, no
There can be block.
3) multistage microwave amplifier：Straw mushroom homogenate in step 2) is added to the water of 22 times of straw mushroom weight, carried out at normal temperatures and pressures
Microwave Extraction, microwave power 350w, Microwave Extraction total time are 15min, interval 1 time, intermittent time 15min, by what is obtained
Material is standby.
4) filter：Material in step 3) is cooled to 40 DEG C, filtering, takes filtrate standby.
5) concentrate：Filtered fluid described in step 4) is concentrated, concentrated using being evaporated under reduced pressure, vacuum 0.09MPa,
Temperature is 70 DEG C, extract solution is concentrated into original volume 1/8, carries out centrifugation impurity elimination, rotating speed 3000r/min, centrifugation time
10min, precipitation is abandoned, obtain straw mushroom polysaccharide and slightly carry concentrate.
6) alcohol precipitation separates：In concentrate, 95% ethanol will be slowly added into described in step 5) to concentration of alcohol up to 30%,
Adding in ethanol process to be stirred continuously, and after staticly settling 20 hours, siphon supernatant to original volume 1/8, lower liquid is carried out
Centrifuge, rotating speed 3500r/min, centrifugation time 12min, 95% ethanol will be continuously added in the supernatant after centrifugation, extremely
Concentration of alcohol stands overnight, centrifuged, obtain two parts precipitation up to 80%.
7) desalination：Add a small amount of water fully to dissolve the precipitation described in step 6), carry out ultrafiltration, ultrafiltration membrane molecule interception
5kD, at 25 DEG C, 2~5kg/m of inlet pressure2, gateway pressure difference do not exceed 1.5kg/m2, obtain trapped fluid.
8) dry：By 50 DEG C of vacuum drying chamber drying of trapped fluid described in step 7).Dried object crushes, and water content is less than
4%, produce anti-oxidant straw mushroom polysaccharide.
9) multistage microwave amplifier straw mushroom polysaccharide has stronger scavenging action and reducing power to OH and DPPH, is above heat
The straw mushroom polysaccharide of water extraction method extraction.Oh resistant ability, Yi Jiyou can be strengthened using ethanol precipitation separation straw mushroom polysaccharide
Stronger DPPH scavenging capacities.
The influence of different time to straw mushroom polysaccharide extract rate under 350w microwave powers of table 2
| Time (min) | 12 | 15 | 18 | 21 | 24 |
| Recovery rate (%) | 14.11 | 14.36 | 9.66 | 9.18 | 8.18 |
| Clearance rate (%) | 75.71 | 75.29 | 70.60 | 69.12 | 63.72 |
Embodiment 3
1) pre-process：The straw mushroom of the non-parachute-opening of selection, clean, impurity elimination, section, 105 DEG C of inactivation 10min.
2) crush：Pretreatment straw mushroom in step 1) is added into agitator, adds the water of 5 times of straw mushroom weight, fully homogenate, no
There can be block.
3) multistage microwave amplifier：Straw mushroom homogenate in step 2) is added to the water of 25 times of straw mushroom weight, carried out at normal temperatures and pressures
Microwave Extraction, microwave power 490w, Microwave Extraction total time are 12min, interval 1 time, intermittent time 20min, by what is obtained
Material is standby.
4) filter：Material in step 3) is cooled to 50 DEG C, filtering, takes filtrate standby.
5) concentrate：Filtered fluid described in step 4) is concentrated, concentrated using atmospheric evaporation, temperature is 100 DEG C, makes to carry
Take liquid to be concentrated into original volume 1/5, be cooled to room temperature, carry out centrifugation impurity elimination, rotating speed 4000r/min, centrifugation time 5min, it is heavy to abandon
Form sediment, obtain straw mushroom polysaccharide and slightly carry concentrate.
6) alcohol precipitation separates：Partial concentration liquid in concentrate, will be taken out described in step 5) and is slowly added into 95% ethanol extremely
Concentration is added in ethanol process and is stirred continuously up to 80%, after staticly settling 16 hours, siphon supernatant to original volume 1/10, under
Portion's liquid is centrifuged, rotating speed 3500r/min, centrifugation time 10min, sediment fraction WPF.Another part concentrate
95% ethanol is slowly added into concentration of alcohol up to 30%, after staticly settling 16 hours, is centrifuged, sediment fraction VWPF30.Will be from
95% ethanol is continuously added in supernatant after the heart, to concentration of alcohol up to 60%, after staticly settling 16 hours, is centrifuged, precipitation portion
It is divided into VWPF60.95% ethanol will be continuously added in supernatant after centrifugation, to concentration of alcohol up to 80%, staticly settled 16 hours
Afterwards, centrifuge, sediment fraction VWPF80.
7) desalination：Adding a small amount of water fully to dissolve the precipitation described in step 6), dialysed, molecule interception is 10kD,
Obtain trapped fluid.
8) dry：Trapped fluid described in step 7) is freeze-dried.Dried object crushes, and water content is less than 4%, produces antioxygen
Change straw mushroom polysaccharide.
9) multistage microwave amplifier straw mushroom polysaccharide has stronger scavenging action and reducing power to OH and DPPH, is above heat
The straw mushroom polysaccharide of water extraction method extraction.Oh resistant ability, Yi Jiyou can be strengthened using ethanol precipitation separation straw mushroom polysaccharide
Stronger DPPH scavenging capacities.
The measure knot of antioxidation activity of the alcohol precipitation separating polyose of the present embodiment with doing positive control with VC (ascorbic acid)
Fruit is referring to Fig. 4,5.As seen from Figure 4, when mass concentration is 2.5mg/mL, the removing OH abilities of alcohol precipitation straw mushroom polysaccharide
It is different notable.Ethanol fractional precipitation straw mushroom polysaccharide VWPF60, VWPF80 is respectively 72.26%, 71.77% to OH scavenging capacities,
It is above non-substep alcohol precipitation straw mushroom polysaccharide WPF 59.40%.As seen from Figure 5, when mass concentration is 2.5mg/mL, alcohol precipitation
The removing DPPH capacity variances of straw mushroom polysaccharide are notable.Ethanol fractional precipitation straw mushroom polysaccharide VWPF30, VWPF60, VWPF80 couple
DPPH scavenging capacities are respectively 73.36%, 56.96%, 64.01%.Ethanol fractional precipitation straw mushroom polysaccharide is removed to DPPH
Activity is slightly below non-substep alcohol precipitation straw mushroom polysaccharide WPF82.71%.
The influence of different time to straw mushroom polysaccharide extract rate under 490w microwave powers of table 3
| Time (min) | 9 | 12 | 15 | 18 | 21 |
| Recovery rate (%) | 6.88 | 12.41 | 11.41 | 8.51 | 8.15 |
| Clearance rate (%) | 76.38 | 79.81 | 76.96 | 75.08 | 59.94 |
Experimental example：
1 hot water extracts straw mushroom polysaccharide method
The straw mushroom of the non-parachute-opening of selection, clean, impurity elimination, section, 105 DEG C of inactivation 10min.The straw mushroom of inactivation is added into stirring
Device, add the water of 10 times of straw mushroom weight, fully homogenate.Straw mushroom homogenate is taken to add the water of 20 times of straw mushroom weight, extracting in boiling water 1h, mistake
Filter.Extracted altogether 2 times by this step, merge extract solution.
The concentrate drying of 2 straw mushroom polysaccharide
Using atmospheric evaporation concentrated extracting solution, temperature is 100 DEG C, extract solution is concentrated into original volume 1/10, is cooled to room
Temperature, centrifugation impurity elimination is carried out, rotating speed 4000r/min, centrifugation time 5min, abandons precipitation.95% ethanol is slowly added into concentrate
To concentration up to 80%, stand overnight, centrifuge (3000r/min, 15min).Precipitation plus a small amount of water are fully dissolved, dialysed
(molecule interception is 10kD), trapped fluid is freeze-dried, hot water is obtained and carries straw mushroom polysaccharide.
Hot water carries straw mushroom polysaccharide yield as 15.07%.
The measure of 3 antioxidation activities
The measure of 3.1OH scavenging capacities
Absorption 0.1,0.5,1.0,1.5,2.0,2.5,5.0mg/mL sample liquid 1.0mL respectively, sequentially add 2mmol/L
FeSO4Solution 3.0mL, 1mmol/LH2O2Solution 3.0mL, stand 10min.Add 6moL/L salicylic acid solutions 3mL, 37 DEG C of water
30min is bathed, absorbance is determined at 510nm, with VC(ascorbic acid) does positive control.Clearance rate calculation formula：Clearance rate
(%)=[1- (Ai-Aj)/A0] × 100%.In formula：AiFor the trap under different polysaccharide concentrations；AjTo replace water with distilled water
The different polysaccharide concentration background absorbances measured during poplar acid；A0Replaced measuring blank control absorbance during polysaccharide sample with water.Often
The parallel average value for doing 3 times, seeking clearance rate of individual concentration.The microwave puies forward polysaccharide sample liquid and extracted using the method in embodiment 1
's.Referring to Fig. 1.
The straw mushroom polysaccharide of table 4 removes OH activity
In the range of 0.1~5mg/mL of mass concentration, hot water extraction straw mushroom polysaccharide is to OH maximal clearances
62.49%.3.2DPPH scavenging capacities determine
Absorption 0.1,0.5,1.0,1.5,2.0,2.5, each 4.0mL of 5.0mg/mL and 0.2mmol/L DPPH solution respectively,
Add in tool plug test tube and shake up, lower 37 DEG C of placements 30min, the 3000r/min centrifugation 10min of dark condition, take supernatant in 517nm
Place surveys absorbance, with VC(ascorbic acid) does positive control.Clearance rate calculation formula：Clearance rate (%)=[1- (Ai-Aj)/A0]
× 100%.In formula：AiFor the trap under polysaccharide concentration；AjTo replace the not homopolysaccharide that is measured during DPPH solution with absolute ethyl alcohol
Concentration background absorbance；A0To be replaced measuring blank control absorbance during polysaccharide sample with water.Each concentration is parallel does 3 times, asks
The average value of clearance rate.The microwave carries what polysaccharide sample liquid was extracted using the method in embodiment 2.Referring to Fig. 2.
The straw mushroom polysaccharide scavenging activity on DPPH of table 5
In the range of 0.1~5mg/mL of mass concentration, hot water extraction straw mushroom polysaccharide is to DPPH maximal clearances
82.71%.
3 reducing powers determine
Take 0.1,0.5,1.0,1.5,2.0,2.5,5.0mg/mL sample liquid 2.5mL, successively plus 0.2moL/L phosphate-buffereds
Solution (pH6.6), 1%K3Fe(CN)6Each 2.5mL of solution, 50 DEG C of water-bath 20min after mixing.It is whole to add 10%TCA solution 2.5mL
Only react, 3000r/min centrifugations 10min.Supernatant 5.0mL is taken, is separately added into distilled water 5.0mL, 0.1%FeCl31.0mL
10min is stood, absorbance is determined at 700nm, each concentration is parallel to be done 3 times, and positive control is done with EDTA.The Microwave Extraction
Polysaccharide sample liquid is extracted using the method in embodiment 3.Referring to Fig. 3.
The straw mushroom polysaccharide reducing power of table 6 measure compares
Straw mushroom polysaccharide has certain reducing power, in the range of 0.1~5mg/mL of mass concentration, 2 kinds of distinct methods extractions
Straw mushroom polysaccharide increases and reducing power increase with mass concentration, and has obvious dose-effect relationship.The reducing power of straw mushroom polysaccharide
There is obvious correlation between its antioxidation activity, the height of reducing power can reflect the strong of oxidation resistance indirectly
It is weak.
Claims (3)
1. a kind of preparation method of anti-oxidant straw mushroom polysaccharide, it is characterised in that this method comprises the following steps：
1）Straw mushroom pre-processes：The straw mushroom of non-self-dissolving is selected, clean, impurity elimination, section, 105 DEG C of inactivation 10-15min；
2）Crush：By step 1）Middle pretreatment straw mushroom adds agitator, adds the water of 5-10 times of straw mushroom weight, fully homogenate；
3）Multistage microwave amplifier；Microwave Extraction total time is 12-18min；
4）Centrifugation or filtering：By step 3）Middle material is cooled to 20-50 DEG C, centrifugal rotational speed 3000-4000r/min, time 5-
10min, or filtering, take clear liquid standby；
5）Concentration：By step 4）Described clear liquid is concentrated, and using ultrafiltration, normal pressure or concentration is evaporated under reduced pressure, makes extract solution dense
Original volume 1/5-1/10 is reduced to, is cooled to room temperature, has precipitation to be centrifuged, rotating speed 3000-4000r/min, centrifugation time
5-10min, precipitation is abandoned, obtain straw mushroom polysaccharide and slightly carry concentrate；It is described that concentration, vacuum 0.07-0.095MPa, temperature is evaporated under reduced pressure
Spend for 60-80 DEG C；Atmospheric evaporation thickening temperature is 90-100 DEG C；
6）Alcohol precipitation separates：In step 5）In described concentrate, 95% ethanol is slowly added into concentration of alcohol up to 30%, adds ethanol
During should be stirred continuously, after staticly settling 6-24 hours, siphon supernatant to original volume 1/5-1/10, lower liquid carry out from
The heart separates, and rotating speed 3500-4000r/min, centrifugation time 10-15min, 95% second will be continuously added in the supernatant after centrifugation
Alcohol, to concentration of alcohol up to 60%, after staticly settling 16 hours, centrifuge, sediment fraction VWPF60；By in the supernatant after centrifugation
95% ethanol is continuously added, to concentration of alcohol up to 80%, after staticly settling 16 hours, is centrifuged, sediment fraction VWPF80；
7）Desalination：By step 6）Described precipitation adds a small amount of water fully to dissolve respectively, carries out ultrafiltration or dialysis, molecule interception are
5-10kD, obtain trapped fluid；
8）Dry：By step 7）Described trapped fluid freeze-drying or 50-60 DEG C of vacuum drying chamber drying, dried object is crushed, contained
Water is less than 4%, and the VWPF60 and VWPF80 obtained after desalination is dried is anti-oxidant straw mushroom polysaccharide.
A kind of 2. preparation method of anti-oxidant straw mushroom polysaccharide according to claim 1, it is characterised in that the step 3）It is micro-
Ripple extracting parameter is：The water of 20-25 times of straw mushroom weight is added in straw mushroom homogenate, carries out Microwave Extraction, microwave at normal temperatures and pressures
Power is 210-490W, and Microwave Extraction total time is 12-18min, interval 1 time, intermittent time 10-20min, the material that will be obtained
It is standby.
A kind of 3. preparation method of anti-oxidant straw mushroom polysaccharide according to claim 1, it is characterised in that the step 5）With
Step 7）Described ultrafiltration, ultrafiltration membrane molecule interception 5kD, at 25 DEG C, 2~5kg/m of inlet pressure2, gateway pressure difference should not
More than 1.5kg/m2。
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