CN105169489A - Multi-layer nanofiber fabric bone tissue engineering scaffold material and preparation method thereof - Google Patents
Multi-layer nanofiber fabric bone tissue engineering scaffold material and preparation method thereof Download PDFInfo
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- CN105169489A CN105169489A CN201510546475.5A CN201510546475A CN105169489A CN 105169489 A CN105169489 A CN 105169489A CN 201510546475 A CN201510546475 A CN 201510546475A CN 105169489 A CN105169489 A CN 105169489A
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Abstract
The invention relates to a multi-layer nanofiber fabric bone tissue engineering scaffold material and a preparation method thereof. The scaffold material is prepared by preparing continuous nanofiber yarns through electrostatic spinning and weaving the yarns by virtue of machine weaving so as to obtain a multi-layer nanofiber fabric. Specifically, the preparation method comprises the following steps: dissolving silk fibroin and a polymer according to different mass ratios in hexafluoroisopropanol so as to obtain a spinning solution which is 6-8% in mass fraction, spinning continuous nanofiber yarns which are 60-500 microns in diameter through electrostatic spinning, and then weaving the yarns by virtue of machine weaving so as to obtain the multi-layer nanofiber fabric, wherein the layer number of the multi-layer nanofiber fabric is equal to or more than 2, a warp density is 50-150 warps/5cm, a weft density is 20-300 wefts/5cm and a porosity is 30-70%. The bone tissue engineering scaffold material disclosed by the invention is simple in preparation method and low in cost, and the scaffold material is excellent in biocompatibility and bone induction property; therefore, the scaffold material can be used as a repair or substitute material for bone tissues.
Description
Technical field
The invention belongs to biology medical material technical field, relate to a kind of multi-layer nano fabric bone tissue engineering stent material and preparation method thereof, be applied to Bone Defect Repari or bone alternate material.
Background technology
Because wound, tumor or congenital development deficiency etc. causes the phenomenon of body bone tissue defect very general, particularly along with China steps into old people society, the old sclerous tissues's pathological changes that easily causes is as osteoporosis.Thus, clinical treatment has huge demand for bone tissue engineer substitution material.Human bone has hierarchy, basic structure is the collagen fiber of height mineralising, the collagenous fiber bundle (or being called array) of further one-tenth mineralising arranged in parallel, the collagenous fiber bundle of these mineralisings forms different pattern of rows and columns in space, and the structure that composition is higher level further.Such as, the feature of lamellar bone is nanometre collagen fibre bundle one-tenth flaggy arranged in parallel, more vertically alternately forms Lamellated structure.Can obtain a kind of timbering material of desirable bionical natural extracellular matrix structure, be one of significant challenge of carrying out of bone tissue engineer.The method that the preparation of current bone tissue engineer substitution material is conventional is: biomineralization, the bionical mixing of composite, natural sponge tissue etc., but there is poor mechanical property, porosity is high, bionic structure is poor problem in the timbering material utilizing these methods to prepare.
Static Spinning is the attractive nanotechnology of a kind of most, is applicable to the nano-fiber material of the many bio-medical aspects of preparation.The orientation utilizing Static Spinning technology to prepare or random nanofiber mats build the application of biomimetic scaffolds in Various Tissues engineering and are well proved, because they can the collagen fento composition of bionic extracellular matrix, this collagen fento is present in various biological tissue cell.But no matter be the problem that orientation or randomly-oriented nanofiber mats all exist poor mechanical property and secondary operations difficulty, be difficult to form structural timbering material.If nanofiber spun yarn is woven into fabric, the problem of nonwoven nanofiber timbering material poor mechanical property and secondary operations difficulty can be solved, simultaneously can the collagen fiber matrix structure of classification orientation in bionical natural lamellar bone.
Summary of the invention
The object of this invention is to provide a kind of preparation method of multi-layer nano fabric bone tissue engineering stent material.Prepare by the method for electrostatic spinning the nano-fibre yams that continuous print is made up of fibroin and polymer, utilize woven method yarn to be woven into the fabric of multilamellar, obtain the support that can be applicable to bone tissue engineer.This bone tissue engineering scaffold not only simulates nature bone on composition, and the nanoscale structurally reached in nature bone and loose structure are conducive to adhesion and the growth of cell, there is excellent mechanical property, good biocompatibility and biological degradability.
The technical scheme realizing the object of the invention is, a kind of multi-layer nano fabric bone tissue engineering stent material, and it is twisted by electrostatic spinning nano fiber to form nano-fibre yams, by woven method, yarns interwoven formed multi-layer nano fabric.
Described nanofiber is made up of fibroin albumen and polymer, and the mass ratio of fibroin albumen and polymer is 1:1-19, the diameter 200-900nm of nanofiber.Described fibroin albumen derives from Bombyxmori Linnaeus silkworm silk and Antherea pernyi Guerin-Meneville silkworm silk, and the intrinsic viscosity [η] of silk fibroin molecular is more than or equal to 0.50.Described polymer is one or more of polyethylene (PE), PGA (PGA), polylactic acid (PLA), Poly-L-lactic acid (PLLA) and Poly(D,L-lactide-co-glycolide (PLGA), and the molecular weight of polymer is more than or equal to 100000.The diameter of described nano-fibre yams is 60-500 μm.The number of plies of described nano fabrics is more than or equal to 2, the end count 100-500 root/5cm of nano fabrics, the 100-500 root/5cm of nano fabrics, the porosity 30-90% of nano fabrics.
The preparation method of multi-layer nano fabric bone tissue engineering stent material, adopts following steps:
(1) by fibroin albumen and polymer in mass ratio for 1:1-19 is dissolved in hexafluoroisopropanol, room temperature lower magnetic force stirs and within 1-5 days, obtains the spinning solution that mass fraction is 6-8%;
(2) electrostatic spinning apparatus is built according to diagram 1, spinning solution in step (1) is joined in syringe pump and prepares continuous print nano-fibre yams, electrostatic spinning voltage is 14-22kV, spinning solution total flow is 0.5-0.9mL/h, the diameter of metal loudspeaker is 10-20cm, the vertical dimension of metal loudspeaker and coiler device is 40-60cm, the vertical dimension of shower nozzle and metal loudspeaker is 4-8cm, the horizontal range of shower nozzle and metal loudspeaker is 3-5cm, the number of shower nozzle is 2-16, shower nozzle internal diameter 0.26-0.86mm, positive and negative shower nozzle liquid inventory compares 1:0.5-1, distance 13-17.5cm between positive and negative shower nozzle, winding speed 40-53mm/min,
(3) utilize woven method to be woven into the nano fabrics of multilamellar the nano-fibre yams of preparation in step (2), the number of plies of multi-layer nano fabric for being more than or equal to 2, end count 50-150 root/5cm, filling density 20-300 root/5cm, porosity 30-70%;
(4) the multi-layer nano fabric prepared in step (3) being immersed in mass fraction is 20-40min in the ethanol water of 80-90%, then dry at 50-60 DEG C in vacuum drying oven after fabric being cleaned 2-3 time in deionized water, obtain multi-layer nano fabric bone tissue engineering stent material.
Compared with existing bone tissue engineering stent material and preparation method thereof, the present invention has the following advantages:
(1) the present invention has prepared nano-fibre yams by the method for electrostatic spinning, utilizes woven method nano-fibre yams to be woven into the nano fabrics of multilamellar to build bone tissue engineering scaffold.The bone tissue engineering stent material of preparation is not only similar to nature bone on composition, and the nanometre collagen microfibrillar structure of classification orientation in structurally better bionical nature bone, has better biocompatibility and self-bone grafting performance;
(2) compare the nanofiber mats of nonwoven, this multi-layer nano fabric timbering material has better mechanical property, fully can meet the demand of Bone Defect Repari or bone grafting material;
(3) in multi-layer nano fabric bone tissue engineering stent material of the present invention, between fiber, between yarn and between tissue layer, or formation rule continuous print space between three, can be distinguished, be conducive to the transport of composition in the adhesion of cell and growth and tissue, the requirement to tissue engineering bracket type in bone collection process can be met.
Accompanying drawing explanation
Fig. 1 is electrostatic spinning apparatus schematic diagram, 1 coiler device, 2 shower nozzles, 3 syringe pumps, 4 metal loudspeaker, 5 high tension generators, 51 positive poles, 52 negative poles;
The SEM photo of the nano-fibre yams of preparation in Fig. 2 embodiment 1;
The photo in kind of the multi-layer nano fabric bone tissue engineering scaffold of preparation in Fig. 3 embodiment 1.
Detailed description of the invention
Below in conjunction with accompanying drawing, by example, the present invention is described in more detail.
A kind of multi-layer nano fabric bone tissue engineering stent material, it is twisted by electrostatic spinning nano fiber to form nano-fibre yams, by woven method, yarns interwoven formed multi-layer nano fabric.Described nanofiber is made up of fibroin albumen and polymer, and the mass ratio of fibroin albumen and polymer is 1:1-19, the diameter 200-900nm of nanofiber.Described fibroin albumen derives from Bombyxmori Linnaeus silkworm silk and Antherea pernyi Guerin-Meneville silkworm silk, and the intrinsic viscosity [η] of silk fibroin molecular is more than or equal to 0.50.Described polymer is one or more of polyethylene (PE), PGA (PGA), polylactic acid (PLA), Poly-L-lactic acid (PLLA) and Poly(D,L-lactide-co-glycolide (PLGA), and the molecular weight of polymer is more than or equal to 100000.The diameter of described nano-fibre yams is 60-500 μm.The number of plies of described nano fabrics is more than or equal to 2, the end count 100-500 root/5cm of nano fabrics, the 100-500 root/5cm of nano fabrics, the porosity 30-90% of nano fabrics.
embodiment 1
A method for multi-layer nano fabric bone tissue engineering stent material, adopts following steps:
The preparation method of multi-layer nano fabric bone tissue engineering stent material, its step is as follows:
(1) be dissolved in hexafluoroisopropanol by tussah silk fibroin and polylactic acid according to mass ratio 1:9, room temperature lower magnetic force stirs and within 2 days, obtains the spinning solution that mass fraction is 6%;
(2) build electrostatic spinning apparatus according to diagram 1, the spinning solution in step (1) is joined in syringe pump 3 and prepares continuous print nano-fibre yams.The spinning voltage of high tension generator 5 is 18kV, spinning solution total flow is 0.6mL/h, the diameter of metal loudspeaker 4 is 15cm, the vertical dimension of metal loudspeaker and coiler device 1 is 40cm, shower nozzle 2 is 5cm with the vertical dimension of metal loudspeaker, the horizontal range of shower nozzle and metal loudspeaker is 3cm, the number of shower nozzle is 4, shower nozzle internal diameter is 0.26mm, the positive pole 51 of high tension generator 5 is connected with positive and negative shower nozzle respectively with negative pole 52, positive and negative shower nozzle liquid inventory is than being 2:1, and the distance between positive and negative shower nozzle is 15cm, and winding speed is 50mm/min;
(3) utilize woven method to be woven into the nano fabrics of multilamellar the nano-fibre yams of preparation in step (2), the number of plies of multi-layer nano fabric is 4 layers, end count 120/5cm, filling density 230/5cm, porosity 60%;
(4) the multi-layer nano fabric prepared in step (3) being immersed in mass fraction is 20min in the ethanol water of 80%.Then dry at 50 DEG C in vacuum drying oven after fabric being cleaned 2 times in deionized water, obtain multi-layer nano fabric bone tissue engineering stent material.
Table 1 shows the mechanical property of multi-layer nano fabric bone tissue engineering stent material prepared by embodiment 1, and the average diameter of yarn and fiber.In multi-layer nano fabric bone tissue engineering scaffold, (in figure, yarn amplification is 500 times for SEM photo such as Fig. 2 of yarn, embedded picture amplification is 6000 times) shown in, as seen from Figure 2, the yarn evenness of bone biomimetic material inside prepared by the present invention is even, and filoplume is less.The photo in kind of multi-layer nano fabric bone tissue engineering scaffold as shown in Figure 3.
embodiment 2
The preparation method of multi-layer nano fabric bone tissue engineering stent material, its step is as follows:
(1) be dissolved in hexafluoroisopropanol by tussah silk fibroin and Poly-L-lactic acid according to mass ratio 1:19, room temperature lower magnetic force stirs and within 3 days, obtains the spinning solution that mass fraction is 7%;
(2) build electrostatic spinning apparatus according to diagram 1, the spinning solution in step (1) is joined in syringe pump and prepares continuous print nano-fibre yams.Spinning voltage is 20kV, spinning solution total flow is 0.9mL/h, the diameter of metal loudspeaker is 10cm, and the vertical dimension of metal loudspeaker and coiler device is 45cm, and the vertical dimension of shower nozzle and metal loudspeaker is 4cm, the horizontal range of shower nozzle and metal loudspeaker is 3cm, the number of shower nozzle is 6, and shower nozzle internal diameter is 0.36mm, and positive and negative shower nozzle liquid inventory is than being 2:1, distance between positive and negative shower nozzle is 16cm, and winding speed is 40mm/min;
(3) utilize woven method to be woven into the nano fabrics of multilamellar the nano-fibre yams of preparation in step (2), the number of plies of multi-layer nano fabric is 2 layers, end count 150/5cm, filling density 250/5cm, porosity 40%;
(4) the multi-layer nano fabric prepared in step (3) being immersed in mass fraction is 10min in the ethanol water of 85%.Then dry at 55 DEG C in vacuum drying oven after fabric being cleaned 2 times in deionized water, obtain multi-layer nano fabric bone tissue engineering stent material.Table 1 shows the mechanical property of multi-layer nano fabric bone tissue engineering stent material prepared by embodiment 2, and the average diameter of yarn and fiber.
embodiment 3
The preparation method of multi-layer nano fabric bone tissue engineering stent material, its step is as follows:
(1) be dissolved in hexafluoroisopropanol by Bombyx mori silk fibroin and Poly(D,L-lactide-co-glycolide according to mass ratio 15:85, room temperature lower magnetic force stirs and within 4 days, obtains the spinning solution that mass fraction is 8%;
(2) build electrostatic spinning apparatus according to diagram 1, the spinning solution in step (1) is joined in syringe pump and prepares continuous print nano-fibre yams.Spinning voltage is 16kV, spinning solution total flow is 0.6mL/h, the diameter of metal loudspeaker is 15cm, and the vertical dimension of metal loudspeaker and coiler device is 40cm, and the vertical dimension of shower nozzle and metal loudspeaker is 3cm, the horizontal range of shower nozzle and metal loudspeaker is 4cm, the number of shower nozzle is 8, and shower nozzle internal diameter is 0.7mm, and positive and negative shower nozzle liquid inventory is than being 1:1, distance between positive and negative shower nozzle is 14cm, and winding speed is 60mm/min;
(3) utilize woven method to be woven into the nano fabrics of multilamellar the nano-fibre yams of preparation in step (2), the number of plies of multi-layer nano fabric is 6 layers, end count 100/5cm, filling density 200/5cm, porosity 70%;
(4) the multi-layer nano fabric prepared in step (3) being immersed in mass fraction is 25min in the ethanol water of 87%.Then dry at 60 DEG C in vacuum drying oven after fabric being cleaned 3 times in deionized water, obtain multi-layer nano fabric bone tissue engineering stent material.Table 1 shows the mechanical property of multi-layer nano fabric bone tissue engineering stent material prepared by embodiment 3, and the average diameter of yarn and fiber.
The performance of a table 1 multi-layer nano fabric bone tissue engineering stent material
Therefore, the multi-layer nano fabric bone tissue engineering stent material that prepared by the present invention has the advantages that fibre orientation is good, yarn evenness is even, fabric mechanics is excellent.The bone tissue engineering stent material of preparation all has good biocompatibility on composition, and the collagen fento matrix structure of classification orientation in structurally bionical nature bone, in timbering material, regular continuous print space is conducive to the transport of composition in the adhesion of cell and growth and tissue.Bone tissue engineering stent material preparation method of the present invention is simple, with low cost, has good biocompatibility and self-bone grafting performance, can as the bone renovating bracket material of embedded type.
Claims (6)
1. a multi-layer nano fabric bone tissue engineering stent material, is characterized in that it prepares continuous print nano-fibre yams by electrostatic spinning, recycles woven method braiding formation multi-layer nano fabric and is prepared from.
2. a kind of multi-layer nano fabric bone tissue engineering stent material according to claim 1, it is characterized in that described nanofiber is made up of fibroin albumen and polymer, fibroin albumen and polymer quality are than being 1:1-19, the diameter 200-900nm of nanofiber, the diameter of described nano-fibre yams is 60-500 μm.
3. a kind of multi-layer nano fabric bone tissue engineering stent material according to claim 1 and 2, it is characterized in that described fibroin albumen derives from Bombyxmori Linnaeus silkworm silk and Antherea pernyi Guerin-Meneville silkworm silk, the intrinsic viscosity of silk fibroin molecular is more than or equal to 0.50.
4. the diameter of the nano-fibre yams described in is 60-500 μm, and described polymer is one or more of polyethylene, PGA, polylactic acid, Poly-L-lactic acid and Poly(D,L-lactide-co-glycolide, and the molecular weight of polymer is more than or equal to 100000.
5. a kind of multi-layer nano fabric bone tissue engineering stent material according to claim 1, it is characterized in that the number of plies of described nano fabrics is more than or equal to 2, the end count 100-500 root/5cm of nano fabrics, 100-500 root/the 5cm of nano fabrics, the porosity 30-90% of nano fabrics.
6. the preparation method of a kind of multi-layer nano fabric bone tissue engineering stent material as claimed in claim 1, is characterized in that carrying out according to the following steps:
(1) by fibroin albumen and polymer in mass ratio for 1:1-19 is dissolved in hexafluoroisopropanol, room temperature lower magnetic force stirs and within 1-5 days, obtains the spinning solution that mass fraction is 6-8%;
(2) electrostatic spinning apparatus is built, spinning solution in step (1) is joined in syringe pump and prepares continuous print nano-fibre yams, electrostatic spinning voltage is 14-22KV, spinning solution total flow is 0.5-0.9ml/h, the diameter of metal loudspeaker is 10-20cm, the vertical dimension of metal loudspeaker and coiler device is 40-60cm, the vertical dimension of shower nozzle and metal loudspeaker is 4-8cm, the horizontal range of shower nozzle and metal loudspeaker is 3-5cm, the number of shower nozzle is 2-16, shower nozzle internal diameter 0.26-0.86mm, positive and negative shower nozzle liquid inventory compares 1:0.5-1, distance 13-17.5cm between positive and negative shower nozzle, winding speed 40-53mm/min,
(3) utilize woven method to be woven into the nano fabrics of multilamellar the nano-fibre yams of preparation in step (2), the number of plies of multi-layer nano fabric is at least 2 layers, end count 50-150 root/5cm, filling density 20-300 root/5cm, porosity 30-70%;
(4) the multi-layer nano fabric prepared in step (3) being immersed in mass fraction is 20-40min in the ethanol water of 80-90%, then dry at 50-60 DEG C in vacuum drying oven after fabric being cleaned 2-3 time in deionized water, obtain multi-layer nano fabric bone tissue engineering stent material.
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| CN105734693A (en) * | 2016-02-02 | 2016-07-06 | 嘉兴学院 | Double-electrode electrostatic spinning device and using method thereof |
| CN106975102A (en) * | 2017-03-08 | 2017-07-25 | 中原工学院 | A kind of bone bionic composite material with negative poisson's ratio structure and preparation method thereof |
| CN107233074A (en) * | 2017-05-11 | 2017-10-10 | 中原工学院 | A kind of flexible wearable nano fabrics sensor and preparation method thereof |
| CN109172871A (en) * | 2018-09-10 | 2019-01-11 | 中原工学院 | A kind of small-bore nano fibrous tissue engineering blood vessel bracket of bilayer and preparation method thereof |
| CN110585487A (en) * | 2019-09-23 | 2019-12-20 | 湖南大学 | Medical periosteum scaffold loaded with ions and geometric pattern signals and construction method thereof |
| CN113388950A (en) * | 2021-06-15 | 2021-09-14 | 青岛大学 | Absorbable high-performance nanofiber woven tendon patch and preparation method thereof |
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| CN109172871A (en) * | 2018-09-10 | 2019-01-11 | 中原工学院 | A kind of small-bore nano fibrous tissue engineering blood vessel bracket of bilayer and preparation method thereof |
| CN110585487A (en) * | 2019-09-23 | 2019-12-20 | 湖南大学 | Medical periosteum scaffold loaded with ions and geometric pattern signals and construction method thereof |
| CN113388950A (en) * | 2021-06-15 | 2021-09-14 | 青岛大学 | Absorbable high-performance nanofiber woven tendon patch and preparation method thereof |
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Application publication date: 20151223 |