CN105168375B - Application of the thinfruit hypecoum herb extract in preparation antibacterials - Google Patents
Application of the thinfruit hypecoum herb extract in preparation antibacterials Download PDFInfo
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Abstract
The present invention relates to a kind of thinfruit hypecoum herb extract preparation antibacterials in application, it is characterised in that: the thinfruit hypecoum herb extract refer to from Papaveraceae erect hypecoum category thinfruit hypecoum herb (HypecoumleptocarpumHook.f.et Thoms.) drying herb in use alcohol extracting, macroreticular resin column purification to obtain total alkaloid content be 5 ~ 60% for 30 ~ 95%, general flavone content extract, in the various dosage forms of the antibacterials of chemical drugs or Chinese medicine or natural drug composition including at least there is the thinfruit hypecoum herb extract.The present invention is by vitro and in vivo it is demonstrated experimentally that thinfruit hypecoum herb extract can be used for preparing the drug of antibacterial.
Description
Technical field
The answering in preparation antibacterials the present invention relates to biomedicine field more particularly to thinfruit hypecoum herb extract
With.
Background technique
When whole body defense function reduces, external world intrusion bacterium or fungi are bred rapidly, easily cause infectious diseases.It is anti-
Raw element is the first choice of bacterial infection disease, and the application of initial antibiotic has many serious bacterial infections of the mankind
The control of effect, but the toxic side effect of antibiotic is quite big, and serious person can cause the side effects such as deafness；Meanwhile antibiosis is used for a long time
Element easily causes internal flora imbalance, induces the pathogenic bacteria of multidrug resistant, the suprainfection of fungi, increases the difficulty in treatment.
The compound with antibacterial action or single preparations of ephedrine being made of natural plants more and more obtain research and confirm,
Because its adverse reaction is relatively light and the advantages that being not likely to produce drug resistance gradually by the attention of scholars.Most of natural products
Antibacterial mechanisms be transfer body in antibacterial active factor and reduce bacterium toxicity and mitigate bacterium to histiocytic
The approach such as destruction play antibacterial action, and a small number of antibacterial mechanisms are that its effective component directly acts on thallus, play inhibition
And killing effect.
In recent years, with the increase of antibody-resistant bacterium, the abuse of antibiotic, antimicrobial natural medicine has become the hot spot of research.
Thinfruit hypecoum herb be Papaveraceae erect hypecoum category thinfruit hypecoum herb (Hypecoumleptocarpum Hook.f.et
Thoms. drying herb) is grown on 4300 meters of height above sea level grassland below, grassy marshland, on sandy ground." Jingzhubencao " records it
Bitter is cool in nature, removing heat from blood, warm and scorchingly hot；" often using Chinese herbal medicine in Tibet " claims it to have the effect of antipyretic-antalgic, eliminating inflammation and expelling toxin, can control
Cold, headache, extremities joint pain, cholecystitis, and solve food poisoning；Claim in " the sweet peaceful green Chinese herbal medicine choosing in Shan ": thin fruit angle
Fennel can control the diseases such as influenza, abscess of throat, hot eyes.
Existing document shows to mainly contain alkaloids and flavone compound in thinfruit hypecoum herb；Its ethyl alcohol extracts
Object can reduce the infiltration of lung tissue inflammatory reaction and inflammatory cell during endotoxin inflammation, reduce peripheral white blood cell, suppression
The release of TNF-α, IL-6 processed；Thinfruit hypecoum herb alkaloid is to Escherichia coli, staphylococcus aureus, bacillus subtilis etc.
Bacterial growth has certain inhibiting effect.
Summary of the invention
Technical problem to be solved by the invention is to provide a kind of thinfruit hypecoum herb extracts in preparation antibacterials
Using.
To solve the above problems, application of the thinfruit hypecoum herb extract of the present invention in preparation antibacterials,
Be characterized in that: the thinfruit hypecoum herb extract refer to from Papaveraceae erect hypecoum category thinfruit hypecoum herb (Hypecoumleptocarpum
Hook.f.et Thoms.) drying herb in use alcohol extracting, macroreticular resin column purification to obtain total alkaloid content for 30 ~
95%, the extract that general flavone content is 5 ~ 60%, it is various with the antibacterials of chemical drugs or Chinese medicine or natural drug composition
Including at least in dosage form has the thinfruit hypecoum herb extract.
The thinfruit hypecoum herb extract is made by following methods:
(1) thinfruit hypecoum herb medicinal material is smashed to 20 ~ 60 mesh, and the volumetric concentration that 6 ~ 15 times of its quality is added is 10 ~ 95%
Ethyl alcohol, at room temperature, extracting times be 2 ~ 4 times, every time 24 ~ 48 hours under conditions of extracted, obtain extracting solution, the extraction
Liquid recycles ethyl alcohol to liquid extract to get thinfruit hypecoum herb extraction through vacuum decompression under conditions of vacuum degree is 0 ~ -0.09MPa
Object；
The thinfruit hypecoum herb extract through macroporous resin column after purification, successively with the water and volume of 3 ~ 10 times of column volumes
Concentration be 60 ~ 95% ethanol elution it is colourless to collection liquid, collect alcohol eluen, the alcohol eluen vacuum degree be 0 ~-
Ethyl alcohol is recycled to dry to get thinfruit hypecoum herb extract through vacuum decompression under conditions of 0.09MPa.
Compared with the prior art, the present invention has the following advantages:
1, thinfruit hypecoum herb extract vitro antibacterial activity in the present invention:
Test strain:
(1) reference culture: 10 plants total
ATCC25922 plants of escherichia coli
ATCC25925 plants of staphylococcus aureus
ATCC27853 plants of Pseudomonas aeruginosa
32209 plants of α-hemolytic streptococcus
32210 plants of beta hemolytic streptococcus
32401 plants of pneumococcus
29010 plants of Neisseria meningitidis
58535 plants of haemophilus influenzae
46104 plants of Klebsiella Pneumoniae
85021 plants of saccharomyces albicans
The above reference culture freeze-drying bacterial strain is purchased from freeze-drying bacterial strain purchased from the offer of Institute of Microorganism, Academia Sinica's strain
The heart.
(2) clinical separation strain: 430 plants total
150 plants of staphylococcus aureus, number: SPC001-SPC150
80 plants of α-hemolytic streptococcus, number: AST001-AST080
80 plants of beta hemolytic streptococcus, number: BST001-BAT080
35 plants of pneumococcus, number: PSS001-PSS035
60 plants of Klebsiella Pneumoniae, number: KLB001-KLB060
25 plants of haemophilus influenzae, number: FLC001-FLC025
The above clinical separation strain is provided by Xi'an Jiaotong University Medical College, through II Full automatic digital Bacteria Identification of Take
Instrument identification.
It tests bacterium solution to prepare: escherichia coli, staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella Pneumoniae inoculation
In MH meat soup, sets 37 DEG C of standard incubator, cultivates within 24 hours；α-hemolytic streptococcus, beta hemolytic streptococcus, pneumonia ball
Bacterium, Neisseria meningitidis, haemophilus influenzae are inoculated in 5% calf serum meat soup, 5%CO237 DEG C of incubator is trained for 48 hours
It supports；Saccharomyces albicans are inoculated in husky guarantor's Ruo Shi meat soup, set 37 DEG C of standard incubator, cultivate within 48 hours；Turbidimetry carries out thin
Bacterium counts, transferred species viable count, i.e. Colony Forming Unit (CFU/ml) in plate measurement bacterium solution.It is deployed into dilution
106CFU/ml bacterium solution is spare.
Test apparatus: CO2Incubator, standard incubator, bacterial colony counting instrument etc..
Culture medium: 5% dextrose bouillon, MH culture medium, Sabouraud' smedium, 5% calf serum meat soup, blood plate, skilful
Gram force plating medium: it is prepared by " Modern microbiological culture medium and reagent handbook ".
1. the measurement of minimal inhibitory concentration (MIC) and minimum bactericidal concentration (MBC):
A measures MIC, MBC of escherichia coli, staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella Pneumoniae:
By thinfruit hypecoum herb extract with the dextrose bouillon containing phenolic red indicator by 20%(W/V) it is that starting point carries out continuously
Two times of gradient dilutions, are sequentially added in 96 porocyte culture plates, the hole 0.2ml/.Adding concentration is 106The test organisms of CFU/ml
The hole liquid 0.01ml/.Bacterium and culture medium control are set up simultaneously.It sets 4 DEG C to act on 12 hours, in 37 DEG C of standard incubator, 24 small
When cultivate, observe result.Minimum drug concentration contained by (culture solution color keeps red) is minimum antibacterial in no bacterial growth hole
Concentration (MIC).The culture for having no each hole of bacterial growth is successively drawn into 0.1ml dibbling in MH plate again, sets standard incubator
It cultivates for 37 DEG C, 24 hours, drug Cmin corresponding to the sterile dibbling region for being born long is minimum bactericidal concentration (MBC).
B is to A type, beta hemolytic streptococcus, MIC, MBC survey of pneumococcus, meningococcus, haemophilus influenzae
It is fixed:
By thinfruit hypecoum herb extract with the 5% calf serum meat soup containing phenolic red indicator by 20%(W/V) it is that starting point carries out
Continuous two times of gradient dilutions, are sequentially added in 96 porocyte culture plates, the hole 0.2ml/.Adding concentration is 106The examination of CFU/ml
Test the hole bacterium solution 0.01ml/.Bacterium and culture medium control are set up simultaneously.It sets 4 DEG C to act on 12 hours, sets 5%CO237 DEG C of incubator,
It cultivates within 48 hours, observes result.Minimum drug concentration contained by (culture solution color keeps red) is minimum in no bacterial growth hole
Mlc (MIC).Will without culture 0.1ml in bacterial growth hole successively dibbling to nutrition plate (α-hemolytic streptococcus,
Beta hemolytic streptococcus, pneumococcus, meningococcus, haemophilus influenzae are inoculated in chocolate plate), set 5%CO2
37 DEG C of incubator is cultivated for 48 hours, and drug Cmin corresponding to the sterile dibbling region for being born long is MBC.
C measures MIC, MBC of saccharomyces albicans:
By thinfruit hypecoum herb extract with husky Ruo Shi (Sabourraud SB) meat soup of protecting containing phenolic red indicator by 20%(W/
V the continuous two times of gradient dilutions of 0.2ml/0.2ml) are carried out in 96 porocyte culture plates for starting point, sequentially add 106CFU/ml's
Bacterium solution 0.01ml, while bacterium and culture medium control are set up, 4 DEG C act on 12 hours；It sets 37 DEG C of standard incubator, train within 48 hours
It supports, observes result.Drug Cmin contained in the hole of no bacterial growth is MIC.It will be without culture in bacterial growth hole
0.1ml successively set 37 DEG C of standard incubator to sand guarantor's Ruo Shi plate, cultivate within 48 hours, the sterile dibbling region for being born long by transferred species
Corresponding drug Cmin is MBC.
2. experimental result:
I thinfruit hypecoum herb extract is shown in Table 1 to MIC, MBC of 10 plants of reference cultures.
MIC and MBC of the 1 thinfruit hypecoum herb extract of table to reference culture
As seen from Table 1, the thinfruit hypecoum herb extract 10 plant reference cultures selected to experiment have obvious inhibition
And deactivation.
MIC, the MIC of II thinfruit hypecoum herb extract to 430 plants of Clinical isolations50、MIC90And MBC is shown in Table 2.
MIC, the MIC of 2 thinfruit hypecoum herb extract of table to 430 plants of Clinical isolations50、MIC90And MBC
Unit: mg/ml
As seen from Table 2, the thinfruit hypecoum herb extract 430 plant Clinical isolations selected to experiment have certain
Inhibit and deactivation, MBC are 2 ~ 4 times of MIC.
It is above-mentioned the experimental results showed that, the thinfruit hypecoum herb extract test strain selected to experiment has certain inhibition
And deactivation, it can be used for the preparation of antibacterials.
2, the present invention in thinfruit hypecoum herb extract vivo bacteria corrosion action:
Control drug: mirabilitum praeparatum buccal tablets, 0.5g/ piece.
Experimental animal: CIR closed colony healthy mice, 18 ± 2g of body, male and female dual-purpose.It is tried purchased from Xi'an Jiaotong University Medical College
Animal center offer is provided.
Test strain: 31001 plants of pneumococcus, 58535 plants of haemophilus influenzae: freeze-drying bacterial strain is micro- purchased from the Chinese Academy of Sciences
Biological study institute's strain provides center.
(1) experiment bacterium minimum lethal dose (MLD) measurement:
CIR mouse is grouped at random, every group 10, male and female dual-purpose.Will it has been determined that viable count (Colony Forming Unit/
Ml 24-48 hours cultures of experiment bacterium) carry out 10 times of continuous gradient dilutions, each dilution bacterium solution collunarium with physiological saline
Infect one group of mouse, infective dose 0.06ml/20g weight.Normal control simultaneously.Raising, observation, record each group dead mouse meticulously
Situation.When control group mice is completely normal, the whole dead smallest bacteria amounts of mouse are minimum lethal dose in cause group in 7 days
(MLD).
The MLD of 31001 plants of pneumococcus infection CIR mouse is 1.5 × 108CFU/kg。
The MLD of 58535 plants of haemophilus influenzae infection CIR mouse is 2.1 × 108CFU/kg。
(2) 0%(ED of the experimental drug to infecting mouse0) and 100%(ED100) the pre- measurement of protection:
CIR mouse is grouped at random, every group 10, male and female dual-purpose.It is small with the MLD amount collunarium infection of experiment bacterium respectively
Mouse.After infecting 12h, 10% medical fluid of thinfruit hypecoum herb extract is done into 5 times of continuous gradient dilutions, each dilution with physiological saline
One group of mouse, dosage 0.5ml/20g weight is injected intraperitoneally in medical fluid.By daily single, successive administration 7 days.It is normal simultaneously right
According to.Raising, observation, dead mouse situation in record 7 days meticulously.When the result of control group is set up completely, mouse is all dead
Maximum dosage-feeding be 0% protective number；The minimum dosage that mouse all survives is 100% protective number.
ED of the thinfruit hypecoum herb extract to 31001 plants of infecting mouses of pneumococcus100For 0.0499 g/kg, ED0For
0.0004 g/kg。
Thinfruit hypecoum herb extract is to 58535 plants of infecting mouse ED of haemophilus influenzae100For 0.0499 g/kg, ED0For
0.0004 g/kg。
(3) 50% protective number (ED50) measurement:
CIR mouse is grouped at random, every group 10, male and female dual-purpose.First with MLD amount test bacteria liquid collunarium infecting mouse, together
When set up positive drug (water melon frost) treatment group, infection control group and sterile infection Normal group, 12 mouse open after infection
Begin to be administered.
By thinfruit hypecoum herb extract 5 gradients of continuous normal saline gradient dilution, each dilution gradient medical fluid abdominal cavity
It is injected in each group mouse of pneumococcal infection, dosage 0.5ml/20g.Positive control drug water melon frost presses 5% suspension 0.5ml/
20g(1.25ml/kg) gastric infusion；Infection control group and Normal group give 0.5ml/20g physiological saline.It is administered daily 1
It is secondary, successive administration 7 days.Raising, observation, record each group dead mouse situation meticulously.Statistics arranges experimental data, calculates thin fruit
ED of the erect hypecoum extract to pneumococcus, haemophilus influenzae clinical separation strain infecting mouse50。
(4) experimental result:
ED of the thinfruit hypecoum herb extract to pneumococcus, acute Haemophilus influenzae infection CIR closed colony mouse50See the table below 3,
Table 4.
3 thinfruit hypecoum herb extract of table is to 31001 plants of infecting mouse In vivoprotective test results of pneumococcus
4 thinfruit hypecoum herb extract of table is to 58535 plants of infecting mouse In vivoprotective test results of haemophilus influenzae
Originally the experimental results showed that, thinfruit hypecoum herb extract is to 31001 plants of pneumococcus and 58535 plants of haemophilus influenzae
The CIR mouse of infection has significant protective effect, can be used for the preparation of antimicrobial.
Specific embodiment
Application of the thinfruit hypecoum herb extract in preparation antibacterials, the thinfruit hypecoum herb extract refer to from Papaveraceae
Erect hypecoum category thinfruit hypecoum herb (HypecoumleptocarpumHook.f.et Thoms.) drying herb in use alcohol
Mention, the total alkaloid content that macroreticular resin column purification obtains is extract that 30 ~ 95%, general flavone content is 5 ~ 60%, with change
It learns to include at least in the various dosage forms of the antibacterials of medicine or Chinese medicine or natural drug composition and has the thinfruit hypecoum herb extract.
Wherein: thinfruit hypecoum herb extract is made by following methods:
(1) thinfruit hypecoum herb medicinal material is smashed to 20 ~ 60 mesh, and the volumetric concentration that 6 ~ 15 times of its quality is added is 10 ~ 95%
Ethyl alcohol, at room temperature, extracting times be 2 ~ 4 times, every time 24 ~ 48 hours under conditions of extracted, obtain extracting solution, the extraction
Liquid recycles ethyl alcohol through vacuum decompression under conditions of vacuum degree is 0 ~ -0.09MPa and slightly mentions to liquid extract to get thinfruit hypecoum herb
Object.
(2) thinfruit hypecoum herb crude extract is through D101 type macroreticular resin column purification, successively with the water and volume of 3 ~ 10 times of column volumes
Concentration be 60 ~ 95% ethanol elution it is colourless to collection liquid, collect alcohol eluen, the alcohol eluen vacuum degree be 0 ~-
Ethyl alcohol is recycled to dry to get thinfruit hypecoum herb extract through vacuum decompression under conditions of 0.09MPa.
Embodiment 1 prepares single medicinal material antibacterial tablet
Thinfruit hypecoum herb medicinal material 1.5kg is taken, is smashed to 20 mesh, the second that the volumetric concentration of 8 times of its quality is 10% is added
Alcohol, at room temperature, extracting times be 3 times, every time 24 hours under conditions of extracted, obtain extracting solution, the extracting solution is true
Reciprocal of duty cycle be -0.09MPa under conditions of through vacuum decompression recycle ethyl alcohol to liquid extract to get thinfruit hypecoum herb crude extract.
Thinfruit hypecoum herb crude extract is through D101 type macroreticular resin column purification, successively with the water and volumetric concentration of 5 times of column volumes
It is colourless to collection liquid for 95% ethanol elution, alcohol eluen is collected, which passes through under conditions of vacuum degree is 0 MPa
Vacuum decompression recycles ethyl alcohol to dry to get thinfruit hypecoum herb extract.
Above-mentioned thinfruit hypecoum herb extract is taken, smashes, sieves with 100 mesh sieve, is 70% with 30% additive amount and total addition level
Excipient (starch, dextrin, sucrose, microcrystalline cellulose, ethyl alcohol, magnesium stearate etc.) mixes, and pelletizes, dry, and whole grain mixes, pressure
Piece is to get the tablet that can treat influenza.
Embodiment 2 prepares single medicinal material antibacterial capsule
Thinfruit hypecoum herb medicinal material 2.5kg is taken, is smashed to 40 mesh, the second that the volumetric concentration of 10 times of its quality is 50% is added
Alcohol, at room temperature, extracting times be 2 times, every time 36 hours under conditions of extracted, obtain extracting solution, the extracting solution is true
Reciprocal of duty cycle be -0.06MPa under conditions of through vacuum decompression recycle ethyl alcohol to liquid extract to get thinfruit hypecoum herb crude extract.
Thinfruit hypecoum herb crude extract is through D101 type macroreticular resin column purification, successively with the water and volumetric concentration of 5 times of column volumes
It is colourless to collection liquid for 70% ethanol elution, alcohol eluen is collected, the alcohol eluen is under conditions of vacuum degree is -0.04MPa
Ethyl alcohol is recycled to dry through vacuum decompression to get thinfruit hypecoum herb extract.
Above-mentioned thinfruit hypecoum herb extract is taken, smashes, sieves with 100 mesh sieve, is 60% with 40% additive amount and total addition level
Excipient (starch, dextrin, sucrose, microcrystalline cellulose, ethyl alcohol, magnesium stearate etc.) mixes, and pelletizes, dry, whole grain, and mixing is filled out
It fills to get the capsule of bacterial pneumonia can be treated.
Embodiment 3 prepares single medicinal material antimicrobial oral liquid
Thinfruit hypecoum herb medicinal material 5kg is taken, is smashed to 20 mesh, the ethyl alcohol that the volumetric concentration of 10 times of its quality is 65% is added,
At room temperature, extracting times are extracted under conditions of being 2 times, every time 36 hours, obtain extracting solution, the extracting solution is in vacuum degree
To recycle ethyl alcohol to liquid extract to get thinfruit hypecoum herb crude extract through vacuum decompression under conditions of -0.05MPa.
Thinfruit hypecoum herb extract is through D101 type macroreticular resin column purification, successively with the water and volumetric concentration of 5 times of column volumes
It is colourless to collection liquid for 95% ethanol elution, alcohol eluen is collected, the alcohol eluen is under conditions of vacuum degree is -0.03MPa
Ethyl alcohol is recycled through vacuum decompression, the liquid extract of relative density 1.2-1.3 is made to get thinfruit hypecoum herb crude extract in extract.
Above-mentioned thinfruit hypecoum herb extract is taken, the corrigent and purified water for being 80% with 20% additive amount and total addition level mix
It is even to get the oral solution that can treat influenza.
Embodiment 4 prepares two taste Chinese medicine antibacterial tablets
Thinfruit hypecoum herb medicinal material 1.5kg is taken, is smashed to 20 mesh, the second that the volumetric concentration of 8 times of its quality is 70% is added
Alcohol, at room temperature, extracting times be 3 times, every time 24 hours under conditions of extracted, obtain extracting solution, the extracting solution is true
Reciprocal of duty cycle be -0.05MPa under conditions of through vacuum decompression recycle ethyl alcohol to liquid extract to get thinfruit hypecoum herb crude extract.
Thinfruit hypecoum herb crude extract is through D101 type macroreticular resin column purification, successively with the water and volumetric concentration of 5 times of column volumes
It is colourless to collection liquid for 95% ethanol elution, alcohol eluen is collected, the alcohol eluen is under conditions of vacuum degree is -0.01MPa
Ethyl alcohol is recycled to dry through vacuum decompression to get thinfruit hypecoum herb extract.
Above-mentioned thinfruit hypecoum herb extract and licorice are taken, smashes, sieves with 100 mesh sieve respectively, mentioned with thinfruit hypecoum herb
Take object: licorice: excipient total amount be 4:2:4 ratio (mass ratio) mix, excipient include starch, dextrin, sucrose,
Microcrystalline cellulose, ethyl alcohol, magnesium stearate etc. are pelletized, dry, and whole grain, mixing, tabletting is to get the piece that can treat influenza
Agent.
Embodiment 5 prepares two taste Chinese medicine antibacterial capsules
Thinfruit hypecoum herb medicinal material 2.5kg is taken, is smashed to 40 mesh, the second that the volumetric concentration of 10 times of its quality is 50% is added
Alcohol, at room temperature, extracting times be 2 times, every time 36 hours under conditions of extracted, obtain extracting solution, the extracting solution is true
Reciprocal of duty cycle be -0.08MPa under conditions of through vacuum decompression recycle ethyl alcohol to liquid extract to get thinfruit hypecoum herb crude extract.
Thinfruit hypecoum herb crude extract is through D101 type macroreticular resin column purification, successively with the water and volumetric concentration of 5 times of column volumes
It is colourless to collection liquid for 70% ethanol elution, alcohol eluen is collected, the alcohol eluen is under conditions of vacuum degree is -0.04MPa
Ethyl alcohol is recycled to dry through vacuum decompression to get thinfruit hypecoum herb extract.
Above-mentioned thinfruit hypecoum herb extract and licorice are taken, smashes, sieves with 100 mesh sieve respectively, mentioned with thinfruit hypecoum herb
Take object: licorice: excipient total amount be 5:3:2 ratio (mass ratio) mix, excipient include starch, dextrin, sucrose,
Microcrystalline cellulose, ethyl alcohol, magnesium stearate etc. are pelletized, dry, whole grain, and mixing is filled to get the glue that can treat bacterial pneumonia
Wafer.
Embodiment 6 prepares two taste Chinese medicine antibacterial oral solutions
Thinfruit hypecoum herb medicinal material 5kg is taken, is smashed to 20 mesh, the ethyl alcohol that the volumetric concentration of 10 times of its quality is 95% is added,
At room temperature, extracting times are extracted under conditions of being 2 times, every time 36 hours, obtain extracting solution, the extracting solution is in vacuum degree
To recycle ethyl alcohol to liquid extract to get thinfruit hypecoum herb crude extract through vacuum decompression under conditions of 0 MPa.
Thinfruit hypecoum herb crude extract is through D101 type macroreticular resin column purification, successively with the water and volumetric concentration of 5 times of column volumes
It is colourless to collection liquid for 95% ethanol elution, alcohol eluen is collected, the alcohol eluen is in the case where vacuum degree is -0.01MPa through vacuum
Ethyl alcohol is recovered under reduced pressure, the liquid extract of relative density 1.2-1.3 is made to get thinfruit hypecoum herb extract in extract.
Above-mentioned thinfruit hypecoum herb extract and liquid extract of liquorice are taken, with thinfruit hypecoum herb extract: liquid extract of liquorice: auxiliary material
The ratio (mass ratio) that (including corrigent and purified water) is 4:1:5 mixes to get the oral solution that can treat influenza.
Above-described embodiment 1 ~ 6 is but not mean that any limitation of the invention to further description of the invention.
In the case where not departing from above-mentioned thought of the invention, the various replacements made according to ordinary skill knowledge and conventional means
Mode or change, are all contained within the present invention.
Claims (1)
1. application of the thinfruit hypecoum herb extract in preparation antibacterials, it is characterised in that: the thinfruit hypecoum herb extract is
Refer to the total alkaloid obtained from the drying herb of Papaveraceae erect hypecoum category thinfruit hypecoum herb using alcohol extracting, macroreticular resin column purification
Content is the extract that 30 ~ 95%, general flavone content is 5 ~ 60%, the antimicrobial formed with chemical drugs or Chinese medicine or natural drug
Including at least in the various dosage forms of object has the thinfruit hypecoum herb extract；
The thinfruit hypecoum herb extract is made by following methods:
(1) thinfruit hypecoum herb medicinal material is smashed to 20 ~ 60 mesh, and the ethyl alcohol that the volumetric concentration of 6 ~ 15 times of its quality is 10 ~ 95% is added,
At room temperature, extracting times are extracted under conditions of being 2 ~ 4 times, every time 24 ~ 48 hours, obtain extracting solution, which exists
Vacuum degree through vacuum decompression recycles ethyl alcohol to liquid extract under conditions of being 0 ~ -0.09MPa；
The liquid extract through macroporous resin column after purification, be successively 60 ~ 95% with the water of 3 ~ 10 times of column volumes and volumetric concentration
Ethanol elution is colourless to collection liquid, collects alcohol eluen, the alcohol eluen is under conditions of vacuum degree is 0 ~ -0.09MPa through true
Ethyl alcohol is recovered under reduced pressure to dry to get thinfruit hypecoum herb extract in sky.
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|---|---|---|---|---|
| CN1616002A (en) * | 2004-09-14 | 2005-05-18 | 马耀茹 | Oral administration or lozenge medicine for treating acute pharyngitis and acute tonsillitis |
| CN1686300A (en) * | 2005-04-11 | 2005-10-26 | 杨凌东科麦迪森制药有限公司 | Ready prepared Chinese medicine used for treating wind heat common cold |
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