CN105146614A

CN105146614A - Functional Chinese dwarf cherry seed ferment, ferment beverage and production method of ferment beverage

Info

Publication number: CN105146614A
Application number: CN201510464172.9A
Authority: CN
Inventors: 周学义; 朱文昭
Original assignee: Individual
Current assignee: Individual
Priority date: 2015-08-01
Filing date: 2015-08-01
Publication date: 2015-12-16
Anticipated expiration: 2035-08-01
Also published as: CN105146614B

Abstract

The invention discloses functional Chinese dwarf cherry seed ferment, a Chinese dwarf cherry seed ferment beverage and a production method of the Chinese dwarf cherry seed ferment beverage. The Chinese dwarf cherry seed ferment consists of the following raw materials in parts by weight: 1-10 parts of Chinese dwarf cherry seeds, 0-5 parts of Chinese wolfberries, 0-1 part of xanthophyll, 0-1 part of taurine, 1-10 parts of honey, 1-10 parts of high fructose corn syrup, 0-1 part of stevioside, and 0-3 parts of oligosaccarides. The production method comprises the following steps: cleaning the Chinese dwarf cherry seeds, pulping the cleaned Chinese dwarf cherry seeds to obtain pulp, and performing enzymatic hydrolysis so as to obtain Chinese dwarf cherry seed juice; pulping the cleaned Chinese wolfberries, performing enzymatic hydrolysis so as to obtain Chinese wolfberry juice; mixing the Chinese dwarf cherry seed juice and the Chinese wolfberry juice so as to obtain mixed juice; sterilizing the mixed juice, and fermenting the sterilized juice; and obtaining Chinese dwarf cherry seed ferment fluid, so that the Chinese dwarf cherry seed ferment beverage is compounded. The Chinese dwarf cherry seed juice ferment and the beverage thereof, disclosed by the invention, are rich in special ingredients, such as calcium, polyphenol, flavone, zeaxanthin, beta-carotene, polysaccharide and the like, and have positive effects of complementing the calcium, softening the blood vessels, improving microcirculation, improving antioxidant ability of organisms, delaying senescence and relieving asthenopia.

Description

A kind of functional calcium fruit ferment, enzyme beverage and production method thereof

Technical field

The invention belongs to biological fermentation field, specifically relate to a kind of functional calcium fruit ferment, enzyme beverage and production method thereof.

Background technology

Calcium fruit also known as Europe Lee, containing rich in protein mineral matter element, vitamin and amino acid.Every hectogram fresh fruit calcium iron content reaches 80 milligrams and 1.5 milligrams respectively, it is 6 times of apple, containing 17 seed amino acids in fruit, total amount is up to 338.3 ~ 451.7mg/100g, and particularly the content of vitamin C, B2 and E and the content of potassium, phosphorus, iron, zinc, selenium and lysine are all higher than existing common fruit variety.The high-class healthy fruit of children and old man pregnant woman.According to parties concerned's investigation, China has population over half to lack Cobastab and calcium.In order to make up the deficiency of this two classes nutriment, current effective way eats calcium fruit exactly, and organic calcium calcium being become easily be absorbed by the body also produces a large amount of Cobastabs.Fruit is called as " star of replenishing the calcium " calcium.

Application number be 200510104930.2 application for a patent for invention disclose a kind of humilis juice and processing method thereof, it adopts high-temp extracting method to extract calcium fruit juice, the nutritive value of calcium fruit and fragrance is run off serious.Application number be 200710062781.7 patent of invention disclose the patent of invention of a kind of cerasus humilis juice beverage and preparation method thereof, it adopts manual stoning, and production efficiency is low, and what produce is a kind of clear beverage.Application number is the patent of invention of 201310202825.7, it discloses Europe Lee's calcium fruit juice, it does not take off core in advance, direct making beating, part fruit stone can be made broken, owing to containing a large amount of amarogentin, fat oil and thick protein in the core of Europe Lee, if in the process of Europe Lee's crushing and beating, core is too much broken, directly will affect the quality of Europe Lee's wine.And at present calcium fruit is not carried out the invention of fermentation for ferment, do not prepare to have yet and replenish the calcium and the calcium fruit ferment of improving eyesight dual-use function and beverage.

At present, more with the patent document that matrimony vine, red date, other fruits and vegetables prepare ferment series products for raw material, such as: application number be 201410326646.9 Chinese patent disclose a kind of preparation method of matrimony vine ferment, with matrimony vine defect ware for raw material, get that juice, enzymolysis, hypoglycemic fermentation, lactobacillus-fermented, ultrasonic cryogenic are concentrated through being separated, allotment, dry, pulverizing and obtain, additive is few, noresidue, achieves the lifting of activity substance content in matrimony vine ferment product.Application number be 201110038192.1 Chinese patent disclose a kind of vinegar beverage and the production method thereof with nourishing qi and blood effect, the quality ratio of components of each material is: Chinese medicine solid particle 1 ~ 5, red date pulp 5 ~ 15, the fruit of Chinese wolfberry 5 ~ 15, sugar 10 ~ 20, vinegar 10 ~ 20; Ginseng, Radix Codonopsis, Chinese yam obtain Chinese medicine solid particle through immersions, infusion, filtration, vaporize draw, then carry out fermentation extraction with the red date pulp of stripping and slicing, the fruit of Chinese wolfberry, sugar, vinegar and obtain, and have effect of nourishing qi and blood, efficient more than 85%.Application number be 201010624400.1 Chinese patent disclose a kind of preparation method of golden jujube ferment, be that raw material obtains through jujube paste preparation, primary enzymolysis, secondary enzymolysis, allotment sterilization, fermentation, freeze drying with golden jujube, the golden jujube ferment of preparation contains abundant protease, lipase, superoxide dismutase, amylase, vitamin, mineral matter and the secondary metabolite such as phenols, flavonoids.Application number be 201410432581.6 Chinese patent disclose a kind of preparation method of environmental protection ferment, adopt raw material to be: apple, mushroom, carrot, hawthorn, the fruit of Chinese wolfberry, lemon and white granulated sugar; Through raw material cleaning, airtight spontaneous fermentation, get filtrate, pure-blood ferment, after-ripening obtain, the preparation method of this environmental protection ferment, change traditional many additives of ferment production and application etc., the ferment produced is not only useful for absorption of human body, and has obvious effect in health care etc.Application number is that the Chinese patent of 201210406990.X discloses a kind of apple ferment, and in mass fraction, it consists of: Radix Codonopsis 40-60 part, north stilbene 15-25 part, red date 30-60 part, fruit of Chinese wolfberry 30-80 part, green apple 400-600 part, oligosaccharides powder 80-100 part, nectar 200-300 part; Preparation method utilizes the plant of pure natural and fruit to produce ferment by spontaneous fermentation, has help and keeps fit, promotes function of immune system, stimulate circulation, and purify the blood whole intestines, accelerates metabolism, keeps the remarkable efficacy such as body stalwartness.As the patent of invention of application number 201310546788.1 " a kind of pectase and preparation method thereof ", disclose with apple, banana, Chinese date, grape, mango, hawthorn, pineapple, tangerine orange, strawberry, ripe gingko, onion as raw material, through cleaning, smash to pieces, enter altar spontaneous fermentation to form for 180 days, there is active material, but unsuitable without protecting.The patent of invention of " the prebiotic vegetable book on Chinese herbal medicine fermented beverage " of application number 201110151867, which disclose prebiotic vegetable book on Chinese herbal medicine fermented beverage and preparation method thereof, this beverage makes extract with red date, the fruit of Chinese wolfberry, cassia seed for raw material, granulated sugar, compound sugar is allocated in extract, and inoculate Lactobacillus plantarum, Pediococcus acidilactici two kinds of lactobacillus-fermenteds, obtain beverage; Wherein, in every 1000kg beverage, have with the red date of dioptrometer>=10%, the fruit of Chinese wolfberry, cassia seed extract 400kg, beverage pH is 3.5, and lactic acid bacteria adds up to 4.9 × 10 ⁸/ ml.

Above-mentioned disclosed patent main direction of studying concentrates in the change of raw material and fermentation mode, and main exist following a few point defect: 1) the spontaneous fermentation cycle is long, cost is high, fermentation pollution risk is large; 2) fermentation mode adopts the fermentation of traditional natural flora, modern composite pure-blood ferment or the combination of the two, too tradition, without innovation; 3) ferment biological Activity and stabill is poor, and the shelf-life is short; 4) mouthfeel and local flavor bad adaptability, appetite are low; 5) ferment with extract, have lost the nutritional labeling of raw material.

Summary of the invention

The object of the invention is to solve the deficiencies in the prior art, provide that a kind of biologically active is high, stability is strong, mouthfeel and raciness, health-care effect significant calcium fruit ferment, enzyme beverage and production method thereof.

Technical scheme of the present invention is: a kind of functional calcium fruit ferment, calcium fruit enzyme beverage and production method thereof, calcium fruit enzyme beverage with the product in calcium fruit ferment production process for raw material; The raw material of calcium fruit ferment consists of: calcium fruit 1-30 part, matrimony vine 0-5 part, lutein 0-1 part, taurine 0-1 part, compound sugar 0-3 part, the some parts of other auxiliary materials, and the production method of described calcium fruit ferment and beverage thereof comprises following steps:

1) calcium fruit cleaning, stoning, preheating, add 0-2 times of water and pull an oar, within 2 hours, calcium fruit juice is obtained at 45-50 DEG C of enzymolysis with pectase and cellulase, calcium fruit juice leaves standstill, and separate section supernatant (subsider juice) is for subsequent use, and its amount is the 0-50% of calcium fruit juice total amount;

2) add 5-10 water doubly after matrimony vine cleaning and carry out rehydration, making beating, then use pectase and cellulase 45-50 DEG C of enzymolysis 2 hours;

3) by step 1) remaining calcium fruit fruit juice and step 2) the wolfberry juice mixing that obtains, carry out ultramicronising process, adjustment mixed serum concentration, makes the soluble solid content of above-mentioned mixed serum reach 2-6%, adjust pH 7-8;

4) by step 3) slurries that obtain add sterilizing after compound sugar, be cooled to 30-40 DEG C, inoculation probio carries out logical oxygen heat-preservation fermentation, supplement HFCS between yeast phase in good time, maintenance soluble solid is stablized, centrifugation after 20-60 hour of fermenting obtains clear liquid and concentrate, and concentrate obtains calcium fruit ferment powder through low temperature drying;

5) clear liquid continues inoculation probio and carries out sealed fermenting, stops fermentation when zymotic fluid ph value reaches 3.0-4.0, obtains calcium fruit ferment stoste after room temperature after ferment 0-30 days;

6) by step 1) isolated calcium fruit supernatant and step 5) the calcium fruit ferment stoste mixing that obtains, then add the auxiliary material such as lutein, taurine, dissolve and mix and obtain a kind of functional calcium fruit ferment;

7) step 5 is got) calcium that obtains fruit ferment stoste 1-30 part; get step 1) isolated calcium fruit supernatant 1-20 part; get lutein 0.1-1 part, taurine 0.1-1 part, other right amount of auxiliary materials; pure water is appropriate, through allotment, homogeneous, degassed, sterilization, fillingly forms calcium really enzyme beverage.

A kind of functional calcium fruit ferment, it is characterized in that being made up of solid, liquid two parts, solid portion is above-mentioned obtained calcium fruit ferment powder, liquid part is above-mentioned calcium fruit ferment, its packaged form is that a flexible package punch is equipped with several solid inner wrappings and forms a complete calcium fruit ferment packaging, drinks time edible by after the solid mixing adding an inner wrapping after a certain amount of liquid diluting.

Described isolated calcium fruit supernatant carries out gelatin, egg-white powder or PVPP process.Gelatin or egg-white powder use amount are 50-100mg/L, are limited with inexcessive, after dissolving, add in calcium fruit supernatant and stir, leave standstill filtration in more than 10 hours; PVPP process consumption 1-5%, add after swelling with the water soakings of 20 times in calcium fruit supernatant and stir, leave standstill after 1 hour and be separated, PVPP can recycle.

A kind of calcium fruit ferment powder is in above-mentioned steps 4) in obtained calcium fruit ferment powder; Or formed through concentrated, drying by calcium fruit ferment stoste or calcium fruit ferment; Also, after can inoculating probiotics fermention by the concentrated calcium fruit juice of calcium fruit through concentrating gained after cleaning, de-core, preheating, making beating, concentrated after adding lutein, taurine, compound sugar, drying forms; Also can pull an oar the calcium fruit juice that obtains through concentrated after cleaning, de-core, preheating by calcium fruit, after adding lutein, taurine, compound sugar, drying forms; Also can by calcium fruit pull an oar after cleaning, de-core, preheating gained the sterilization of calcium fruit juice after inoculate probiotics fermention, concentrated after adding lutein, taurine, compound sugar after fermentation ends, drying forms; Also can by calcium fruit after cleaning, de-core, preheating through dry, pulverize after add lutein, taurine, compound sugar mix and form.

A kind of calcium chankings, is processed by calcium fruit ferment powder; Calcium chankings comprises effervescent tablet, chewable tablets, lozenge; Calcium fruit ferment powder also can be processed into the product form such as particle electuary, capsule.

Described probio is bifidobacterium adolescentis, animal bifidobacteria (bifidobacterium lactis), bifidobacterium bifidum, bifidobacterium breve, bifidobacterium infantis, bifidobacterium longum, bacillus coagulans, Lactobacillus casei, Lactobacillus crispatus, lactobacillus delbruockii subspecies bulgaricus (lactobacillus bulgaricus), bacillus cereus, Lactobacillus delbrueckii breast subspecies, lactobacillus fermenti, lactobacillus gasseri, Lactobacillus helveticus, Yue Shi lactobacillus, lactobacillus paraceasi, lactobacillus reuteri, Lactobacillus rhamnosus, bacillus subtilis, Lactobacillus salivarius, streptococcus thermophilus, Lactobacillus plantarum, bacillus licheniformis, lactobacillus paraceasi, Lactobacillus rhamnosus, lactobacillus acidophilus, leuconostoc mesenteroides subsp mesenteroides, kluyveromyces marxianus, Pediococcus acidilactici, one or more in Pediococcus pentosaceus.

Probio is not toxigenic bacterial strain that state approval can be used for food or medicine.

By calcium pulp through composition adjustment, sterilization, Vacuum Concentration, dry and the mixing powder containing a large amount of lactic acid thalline and metabolite thereof made after inoculating probiotics fermention, function of dominant type calcium fruit product, is rich in prebiotic rhzomorph, can makes health food or food additives.

Dry employing microwave drying, vacuum drying, vacuum freeze drying etc.

Calcium fruit ferment stoste or the concentrated cryogenic vacuum that adopts of calcium fruit ferment concentrate, baking temperature no more than 50 DEG C, the active component to greatest extent in reservation process material.

Above-mentioned compound sugar is one or more in xylo-oligosaccharide, FOS, oligoisomaltose, galactooligosaccharide, stachyose, soyabean oligosaccharides, gossypose, oligomeric maltose.

Granulated sugar, HFCS or honey is adopted during soluble solid adjustment.

In addition, fresh calcium fruit through cleaning, go out enzyme or the enzyme that do not go out, broken, keeps fruit stone complete, add pectase and cellulase insulation enzymolysis, period continuous extrusion stirring, at the end of enzymolysis, adds after 1-2 water doubly stirs and leaves standstill 30 minutes, add other raw material, through sterilizing after mixing, microbe inoculation ferments, and can isolate calcium fruit ferment Normal juice after fermentation ends.

In addition, fresh calcium fruit through cleaning, go out enzyme or the enzyme that do not go out, attrition crushing pulp, keep fruit stone not to be broken, spontaneous fermentation is mixed in sugaring thoroughly, is separated and obtains calcium fruit ferment Normal juice after fermentation ends.

Fresh calcium fruit is dried, and through cleaning when ferment is produced, rehydration, attrition crushing under the condition not destroying fruit stone, sterilizing, inoculation probio is fermented, and fermentation liquor centrifugation is sloughed fruit stone and obtained calcium fruit ferment Normal juice.

Calcium fruit ferment can be preserved in sterilizing.Also by adding HFCS or white granulated sugar, the soluble solid of calcium fruit ferment stoste can be adjusted to 50-70%, the prebiotic rhzomorph produced by hyperosmosis and probio suppresses microbial activity, makes calcium fruit ferment be able to long-term preservation.

Sterilizing after raw material pulping, miniaturization process, adjustment soluble solid content and Ph value, the probiotics fermentions such as inoculation bacillus coagulans, ferment after 20-40 days, through allotment, then with granulated sugar or HFCS, zymotic fluid soluble solid content is adjusted to about 60%, bottling is calcium fruit ferment.

Invention achieves following effect:

Because the fruit of Chinese wolfberry contains the required nutrition of the healthy eye such as calcium, iron such as abundant carrotene, zeaxanthin, physalin, retinol1, B1, B2, C, therefore be good at improving eyesight, so be commonly called as " bright eye ".Anthocyanidin in Combined with Calcium fruit, lutein, taurine, can play good improving eyesight function.

Probio during the fermentation, because microorganism promotes that raw material metabolism produces a large amount of elementary, secondary metabolite and as outside the organic active ingredients such as amino acid, vitamin, organic acid, various enzyme, aromatic compound, sugar alcohol, polyphenol, polysaccharide, small-molecular peptides and a large amount of compound sugar, but also produces the many valuable factor with health role to the mankind such as a large amount of probiotic cells, hepatocuprein (SOD), eicosapentaenoic acid (EPA), DHA (DHA) and flavone compound.Calcium fruit ferment produces a large amount of nutrition contained by the Small molecular functional components such as a large amount of lacteins and raw material self and functional component at lactic acid bacteria fermentation process, these beneficiating ingredients promote the increment of intestinal beneficial flora greatly, suppress harmful bacteria, thus reduce the generation of corrupt substance and noxious material, playing functional while, also play regulating intestinal canal colony balance, the effect strengthen immunity, promoting beauty treatment, promote sleep, delay senility.

The present invention is due to the biochemical reaction of raw material enzymolysis processing and multi-strain fermentation, and the anthocyanidin in matrimony vine raw material, zeaxanthin and ester class thereof are separated with pulp, and in free state, be conducive to human body and better absorb, improving eyesight function is better.

The present invention adopts multi-cultur es combined ferment to form, and produces a large amount of raw material metabolites and thalline autolysate and a large amount of somatic cells, has important function to raising immunity of organisms.

Of the present invention containing abundant soluble dietary fiber, compound sugar, containing a large amount of probios, makes product have whole intestines defaecation, the endotoxic good effects of purged body, long-term eating can reach lowering blood-fat and reducing weight, toxin-expelling and face nourishing, control colorectal cancer, the medical value preventing and treating heart disease and health-care effect.

The present invention contains nutrients such as enriching calcium, phosphorus, potassium, magnesium, iron, zinc, vitamin C, B family vitamin, has important function to maintenance body normal function.

The present invention also containing the abundant special composition such as polyphenol, flavones, zeaxanthin, anthocyanidin, beta carotene, polysaccharide, to softening blood vessel, improves microcirculation, improves antioxidant ability of organism, delays senility, alleviating asthenopia has positive role.

Ferment of the present invention and beverage all remain the fragrance of calcium fruit, and products taste and function all get a promotion.

The invention solves that the de-core efficiency of prior art calcium fruit is low, equipment investment large, realize calcium fruit ferment suitability for industrialized production.

With specific embodiment, technical scheme of the present invention is described further below.

Detailed description of the invention

Embodiment 1

A kind of raw material of functional calcium fruit ferment consists of: calcium fruit 30 parts, matrimony vine 5 parts, 1 part, lutein, taurine 1 part, honey 10 parts, HFCS 10 parts, granulated sugar 40 parts, xylo-oligosaccharide 3 parts.

The production method of functional calcium fruit ferment and enzyme beverage thereof comprises following steps:

1) calcium fruit cleaning, stoning, preheating, add the making beating of 2 times of water, with pectase and cellulase 45-50 DEG C of enzymolysis 2 hours calcium fruit juice, calcium fruit juice leaves standstill, and separate section supernatant (subsider juice) is for subsequent use, and its amount is 50% of calcium fruit juice total amount;

2) water adding 10 times after matrimony vine cleaning carries out rehydration, making beating, then uses pectase and cellulase 45-50 DEG C of enzymolysis 2 hours;

3) by step 1) really fruit juice and the wolfberry juice mixing of remaining calcium, carry out ultramicronising process, adjustment mixed serum concentration, make the soluble solid content of above-mentioned mixed serum reach 2%, adjust pH is 7;

4) by step 3) add compound sugar in the slurries that obtain, be cooled to 30 DEG C after sterilizing, inoculation probio carries out logical oxygen, heat-preservation fermentation, and the stage supplements HFCS, and maintaining solid content is 2%; Centrifugation after 20 hours of fermenting obtains clear liquid and concentrate, and concentrate obtains calcium fruit ferment powder through low temperature drying; Probio is bifidobacterium infantis, bifidobacterium longum, bacillus coagulans, lactobacillus fermenti, Yue Shi lactobacillus, bacillus licheniformis, lactobacillus acidophilus, Pediococcus acidilactici, and each bacterial classification equal proportion uses, and total consumption is 5/1000ths.

5) clear liquid continues inoculation probio and carries out sealed fermenting, stops fermentation when zymotic fluid ph value reaches 4.0, and after room temperature, ferment obtains calcium fruit ferment stoste after 30 days; Probio is bifidobacterium infantis, bifidobacterium longum, lactobacillus fermenti, Yue Shi lactobacillus, lactobacillus acidophilus, Pediococcus acidilactici, and each bacterial classification equal proportion uses, and total consumption is 5/1000ths.

6) in step 5) described in calcium fruit ferment stoste in add step 1) isolated calcium fruit supernatant, add lutein, taurine, stevioside, syrup, honey, carry out sterilization treatment after dissolving and namely obtain a kind of functional calcium fruit ferment.

7) step 5 is got) calcium that obtains fruit ferment stoste 30 parts, get step 1) isolated calcium fruit supernatant 20 parts, get 1 part, lutein, taurine 1 part, stevioside 1 part, oligoisomaltose 2 parts, honey is appropriate, HFCS is appropriate, stabilizing agent is appropriate, and pure water is appropriate; Through allotment, homogeneous, degassed, sterilization, fillingly form a kind of functional calcium fruit enzyme beverage.

Another kind of calcium fruit ferment is made up of solid, liquid two parts, solid portion is above-mentioned calcium fruit ferment powder, liquid part is above-mentioned calcium fruit ferment, its packaged form is that a flexible package punch is equipped with several solid inner wrappings composition calcium fruit ferment packaging, drinks after being mixed by the solid adding an inner wrapping time edible after a certain amount of liquid diluting.

Calcium fruit ferment stoste is filtered, then produces clarification calcium fruit ferment and clarification calcium fruit enzyme beverage.

Calcium fruit ferment stoste and calcium fruit ferment are formed calcium fruit ferment powder through concentrated, microwave drying respectively.

Also, after can inoculating above-mentioned probiotics fermention by the concentrated calcium fruit juice of calcium fruit through concentrating gained after cleaning, de-core, preheating, making beating, sterilization, concentrated after adding lutein, taurine, compound sugar, drying forms calcium fruit ferment powder.

Also can inoculate above-mentioned probiotics fermention by calcium fruit after the calcium fruit juice sterilization of cleaning, de-core, preheating, making beating gained, after adding lutein, taurine, compound sugar, form calcium fruit ferment powder through concentrated, microwave drying.

Process calcium chankings by above-mentioned five kinds of calcium fruit ferment powders respectively, calcium chankings comprises effervescent tablet, chewable tablets, lozenge.

Above-mentioned five kinds of calcium fruit ferment powder also can be processed into the product form such as particle electuary, capsule.

Embodiment 2

A kind of raw material of functional calcium fruit ferment consists of: calcium fruit 1 part, matrimony vine 0.5 part, 0.1 part, lutein, taurine 0.2 part, honey 1 part, HFCS 2 parts, granulated sugar 3 parts, oligoisomaltose 0.6 part.

The production method of functional calcium fruit ferment, enzyme beverage comprises following steps:

1) cleaning of calcium fruit, stoning, preheating, making beating, within 2 hours, obtain calcium fruit juice with pectase and cellulase at 45-50 DEG C of enzymolysis, calcium fruit juice leaves standstill, and separate section supernatant (subsider juice) is for subsequent use, and its amount is calcium fruit juice total amount 20%;

2) water adding 5 times after matrimony vine cleaning carries out rehydration, making beating, then uses pectase and cellulase 45-50 DEG C of enzymolysis 2 hours;

3) by step 1) really fruit juice and the wolfberry juice mixing of remaining calcium, carry out ultramicronising process, adjustment mixed serum concentration, make the soluble solid content of above-mentioned mixed serum reach 6%, adjust pH is 8;

4) by step 3) add compound sugar in the slurries that obtain, be cooled to 40 DEG C after sterilizing, inoculation probio carries out logical oxygen, heat-preservation fermentation, and the stage supplements HFCS, and maintaining solid content is 6%; Centrifugation after 60 hours of fermenting obtains clear liquid and concentrate, and concentrate obtains calcium fruit ferment powder through low temperature drying; Probio is bifidobacterium bifidum, bacillus coagulans, Lactobacillus casei, Lactobacillus helveticus, bacillus licheniformis, Lactobacillus rhamnosus, bacillus subtilis, Pediococcus acidilactici, Pediococcus pentosaceus, each bacterial classification equal proportion uses, and total consumption is 5 percent.

5) clear liquid continues inoculation probio and carries out sealed fermenting, stops fermentation when zymotic fluid ph value reaches 3.0, obtains calcium fruit ferment stoste; Probio is bifidobacterium bifidum, Lactobacillus casei, Lactobacillus rhamnosus, bacillus subtilis, Pediococcus acidilactici, Pediococcus pentosaceus, and each bacterial classification equal proportion uses, and total consumption is 5/1000ths.

6) in step 4) described in calcium fruit ferment stoste in add step 1) isolated calcium fruit supernatant, add lutein, taurine, syrup, honey and granulated sugar, after dissolving, namely obtain a kind of functional calcium fruit ferment.

7) get calcium fruit ferment stoste 1 part, isolated calcium fruit supernatant 1 part, 1 part, lutein, taurine 1 part, stevioside 1 part, oligoisomaltose 2 parts, honey is appropriate, HFCS is appropriate, and in right amount, pure water is appropriate for stabilizing agent; Through allotment, homogeneous, degassed, sterilization, fillingly form a kind of functional calcium fruit enzyme beverage.

Clarification calcium fruit enzyme beverage can be produced after calcium fruit ferment stoste being filtered.

Add gelatin or the egg-white powder solution of 80mg/L in isolated calcium fruit supernatant, stir, leave standstill filtration in more than 10 hours.

Calcium fruit ferment stoste and calcium fruit ferment form calcium fruit ferment powder through concentrated, vacuum freeze drying respectively, and calcium fruit ferment stoste and the concentrated cryogenic vacuum that all adopts of calcium fruit ferment concentrate, temperature no more than 50 DEG C, the active component to greatest extent in reservation process material.

Embodiment 3

A kind of raw material of functional calcium fruit ferment consists of: calcium fruit 30 parts, matrimony vine 0 part, 0 part, lutein, taurine 0 part, honey 2 parts, HFCS 2 parts, granulated sugar 30 parts, xylo-oligosaccharide 0 part.

1) calcium fruit cleaning, stoning, preheating, add the making beating of 1.5 times of water, with pectase and cellulase 45-50 DEG C of enzymolysis 2 hours calcium fruit juice, calcium fruit juice leaves standstill, and separate section supernatant (subsider juice) is for subsequent use, and its amount is 50% of calcium fruit juice total amount;

2) by step 1) remaining calcium fruit fruit juice carries out ultramicronising process, adjustment mixed serum concentration, and make the soluble solid content of above-mentioned mixed serum reach 4%, adjust pH is 7.5;

3) by step 2) add xylo-oligosaccharide in the slurries that obtain, be cooled to 37 DEG C after sterilizing, inoculation probio carries out logical oxygen, heat-preservation fermentation, and the stage supplements HFCS, and maintaining solid content is 4%; Centrifugation after 40 hours of fermenting obtains clear liquid and concentrate, and concentrate obtains calcium fruit ferment powder through low temperature drying; Probio is bifidobacterium bifidum, bacillus coagulans, Lactobacillus casei, Lactobacillus helveticus, bacillus licheniformis, Lactobacillus rhamnosus, bacillus subtilis, Pediococcus acidilactici, Pediococcus pentosaceus, each bacterial classification equal proportion uses, and total consumption is 5 percent.

4) clear liquid continues inoculation probio and carries out sealed fermenting, stops fermentation when zymotic fluid ph value reaches 3.5, and after room temperature, namely ferment obtains calcium fruit ferment stoste for 20 days; Probio is bifidobacterium bifidum, bacillus coagulans, Lactobacillus casei, Lactobacillus helveticus, bacillus licheniformis, Lactobacillus rhamnosus, bacillus subtilis, Pediococcus acidilactici, Pediococcus pentosaceus, each bacterial classification equal proportion uses, and total consumption is 10/1000ths.

5) in step 4) described in calcium fruit ferment stoste in add step 1) isolated calcium fruit supernatant, add syrup, honey and granulated sugar, after dissolving, namely obtain a kind of functional calcium fruit ferment.

6) get calcium fruit ferment stoste 30 parts, isolated calcium fruit supernatant 10 parts, 1 part, lutein, taurine 1 part, stevioside 1 part, xylo-oligosaccharide 2 parts, honey is appropriate, HFCS is appropriate, and in right amount, pure water is appropriate for stabilizing agent; Through allotment, homogeneous, degassed, sterilization, fillingly form a kind of functional calcium fruit enzyme beverage.

Calcium fruit ferment stoste and calcium fruit ferment form calcium fruit ferment powder through concentrated, vacuum drying respectively, and calcium fruit ferment stoste and the concentrated cryogenic vacuum that all adopts of calcium fruit ferment concentrate, temperature no more than 50 DEG C, the active component to greatest extent in reservation process material.

Embodiment 4

A kind of raw material of functional calcium fruit ferment consists of: calcium fruit 20 parts, matrimony vine 2 parts, 0.2 part, lutein, taurine 0.3 part, honey 2 parts, HFCS 1 part, granulated sugar 20 parts, FOS 0.4 part;

1) calcium fruit cleaning, stoning, preheating, add the making beating of 1 times of water, with pectase and cellulase 45-50 DEG C of enzymolysis 2 hours calcium fruit juice, calcium fruit juice is standing;

2) water adding 8 times after matrimony vine cleaning carries out rehydration, making beating, then uses pectase and cellulase 45-50 DEG C of enzymolysis 2 hours;

3) by step 1) calcium fruit fruit juice and step 2) wolfberry juice mixing, carry out ultramicronising process, adjustment mixed serum concentration, make the soluble solid content of above-mentioned mixed serum reach 3%, adjust pH is 7;

4) by step 3) add FOS in the slurries that obtain, be cooled to 35 DEG C after sterilizing, inoculation probio carries out logical oxygen, heat-preservation fermentation, and stage interval supplements HFCS, and maintaining solid content is 3%; Centrifugation after 30 hours of fermenting obtains clear liquid and concentrate, and concentrate obtains calcium fruit ferment powder through low temperature drying; Probio is bifidobacterium bifidum, bifidobacterium infantis, Lactobacillus crispatus, bacillus coagulans, Yue Shi lactobacillus, Lactobacillus rhamnosus, Lactobacillus salivarius, lactobacillus paraceasi, bacillus licheniformis, lactobacillus acidophilus, each bacterial classification equal proportion uses, and total consumption is 2 percent.

5) clear liquid continues inoculation probio and carries out sealed fermenting, stops fermentation when zymotic fluid ph value reaches 3.5, and after room temperature, namely ferment obtains calcium fruit ferment stoste for 10 days; Probio is bifidobacterium bifidum, bifidobacterium infantis, Lactobacillus crispatus, bacillus coagulans, Yue Shi lactobacillus, Lactobacillus rhamnosus, Lactobacillus salivarius, lactobacillus paraceasi, bacillus licheniformis, lactobacillus acidophilus, each bacterial classification equal proportion uses, and total consumption is 2/1000ths.

6) step 1 is added in calcium fruit ferment stoste) isolated calcium fruit supernatant, then add lutein, taurine, granulated sugar, syrup, honey, dissolve, obtain a kind of functional calcium fruit ferment.

7) get calcium fruit ferment stoste 10 parts, 1 part, lutein, taurine 1 part, stevioside 1 part, FOS 2 parts, honey is appropriate, HFCS is appropriate, and in right amount, pure water is appropriate for stabilizing agent; Through allotment, homogeneous, degassed, sterilization, fillingly form a kind of functional calcium fruit enzyme beverage.

Clarification enzyme beverage can be produced after calcium fruit ferment stoste being filtered.

Embodiment 5

A kind of raw material of functional calcium fruit ferment consists of: calcium fruit 5 parts, matrimony vine 2 parts, 0.2 part, lutein, taurine 0.3 part, honey 2 parts, HFCS 1 part, granulated sugar 10 parts, FOS 0.4 part;

1) calcium fruit cleaning, stoning, preheating, add the making beating of 1 times of water, with pectase and cellulase 45-50 DEG C of enzymolysis 2 hours calcium fruit juice, calcium fruit juice leaves standstill, and filters and to obtain calcium fruit subsider juice, the filtration in 1 hour of the PVPP process with 3%;

4) by step 3) add FOS in the slurries that obtain, be cooled to 35 DEG C after sterilizing, inoculation probio carries out logical oxygen, heat-preservation fermentation, and stage interval supplements HFCS, and maintaining solid content is 3%; Probio is animal bifidobacteria (bifidobacterium lactis), bifidobacterium breve, bacillus licheniformis, lactobacillus gasseri Lactobacillus salivarius, Lactobacillus plantarum, bacillus coagulans, Lactobacillus rhamnosus, bacillus subtilis, each bacterial classification equal proportion uses, and total consumption is 3 percent.

5) stop fermentation after fermenting 30 hours, obtain calcium fruit ferment stoste;

6) in calcium fruit ferment stoste, add lutein, taurine, honey, adjust soluble solid content to be 62% with syrup, granulated sugar, obtain a kind of functional calcium fruit ferment.

7) get calcium fruit ferment stoste 15 parts, calcium fruit subsider juice 5 parts, 1 part, lutein, taurine 1 part, stevioside 1 part, FOS 2 parts, honey is appropriate, HFCS is appropriate, and in right amount, pure water is appropriate for stabilizing agent; Through allotment, homogeneous, degassed, sterilization, fillingly form a kind of functional calcium fruit enzyme beverage.

Process calcium chankings by above-mentioned four kinds of calcium fruit ferment powders respectively, calcium chankings comprises effervescent tablet, chewable tablets, lozenge.

Above-mentioned four kinds of calcium fruit ferment powder also can be processed into the product form such as particle electuary, capsule.

Embodiment 6

A kind of raw material of functional calcium fruit ferment consists of: calcium fruit 15 parts, matrimony vine 2 parts, 0.15 part, lutein, taurine 0.25 part, honey 1 part, HFCS 2 parts, granulated sugar 15 parts, galactooligosaccharide 0.6 part.

1) calcium fruit cleaning, stoning, preheating, add the making beating of 2 times of water, with pectase and cellulase 45-50 DEG C of enzymolysis 2 hours calcium fruit juice, calcium fruit juice leaves standstill, and separate section supernatant (subsider juice) is for subsequent use, and its amount is 20% of calcium fruit juice total amount;

3) by step 1) really fruit juice and the wolfberry juice mixing of remaining calcium, carry out ultramicronising process, adjustment mixed serum concentration, make the soluble solid content of above-mentioned mixed serum reach 5%, adjust pH is 7.5;

4) by step 3) add galactooligosaccharide in the slurries that obtain, be cooled to 37 DEG C after sterilizing, inoculation probio carries out logical oxygen, heat-preservation fermentation, and the stage supplements HFCS, and maintaining solid content is 5%; Centrifugation after 40 hours of fermenting obtains clear liquid and concentrate, and concentrate obtains calcium fruit ferment powder through low temperature drying; Probio is bifidobacterium adolescentis, bacillus coagulans, Lactobacillus casei, bacillus cereus, bacillus licheniformis, streptococcus thermophilus, Pediococcus pentosaceus, and each bacterial classification equal proportion uses, and total consumption is one of percentage.

5) in step 4) supernatant of gained inoculation probio continues fermentation, and stop fermentation when zymotic fluid ph value reaches 3.0, after room temperature, namely ferment obtains calcium really ferment stoste for 15 days; Probio is bifidobacterium breve, Lactobacillus plantarum, Lactobacillus casei, lactobacillus acidophilus, and each bacterial classification equal proportion uses, and total consumption is 5/1000ths.

6) in step 5) add step 1 in the calcium that obtains fruit ferment stoste) isolated calcium fruit supernatant; then galactooligosaccharide, lutein, taurine is added; add granulated sugar, syrup, honey, after dissolving, bottling sterilization, namely obtain a kind of functional calcium fruit ferment.

7) get calcium fruit ferment stoste 10 parts, isolated calcium fruit supernatant 5 parts, 1 part, lutein, taurine 1 part, stevioside 1 part, oligoisomaltose 2 parts, honey is appropriate, HFCS is appropriate, and in right amount, pure water is appropriate for stabilizing agent; Through allotment, homogeneous, degassed, sterilization, fillingly form a kind of functional calcium fruit enzyme beverage.

Get the PVPP of 4%, add after swelling with the water soakings of 20 times isolated calcium fruit supernatant in stir, leave standstill after 1 hour after separating and filtering for Beverage Service, PVPP can recycle.

Embodiment 7

A kind of raw material of functional calcium fruit ferment consists of: calcium fruit 10 parts, matrimony vine 1 part, 0.15 part, lutein, taurine 0.15 part, honey 1 part, HFCS 2 parts, granulated sugar 12 parts, soyabean oligosaccharides 0.6 part.

3) by step 1) remaining calcium fruit fruit juice and step 2) the wolfberry juice mixing that obtains, carry out ultramicronising process, adjustment mixed serum concentration, make the soluble solid content of above-mentioned mixed serum reach 2%, adjust pH is 7.5;

4) by step 3) add soyabean oligosaccharides in the slurries that obtain, be cooled to 37 DEG C after sterilizing, inoculation probio carries out logical oxygen, heat-preservation fermentation, and the stage supplements HFCS, and maintaining solid content is 2%; Centrifugation after 30 hours of fermenting obtains clear liquid and concentrate, and concentrate obtains calcium fruit ferment powder through low temperature drying; Probio is bacillus coagulans, and consumption is 2 percent.

5) clear liquid continues inoculation probio and carries out sealed fermenting, stops fermentation when zymotic fluid ph value reaches 3.5, and after room temperature, namely ferment obtains calcium fruit ferment stoste for 20 days; Probio is bifidobacterium bifidum, bacillus coagulans, Lactobacillus casei, Lactobacillus helveticus, Lactobacillus rhamnosus, Pediococcus acidilactici, Pediococcus pentosaceus, and each bacterial classification equal proportion uses, and total consumption is 10/1000ths.

6) in step 5) described in calcium fruit ferment stoste in; add step 1) isolated calcium fruit supernatant; add lutein, taurine, honey, reach 62% with syrup, granulated sugar adjustment soluble solid content, obtain a kind of functional calcium fruit ferment.

7) get calcium fruit ferment stoste 10 parts, isolated calcium fruit supernatant 5 parts, 1 part, lutein, taurine 1 part, stevioside 1 part, soyabean oligosaccharides 2 parts, honey is appropriate, HFCS is appropriate, and in right amount, pure water is appropriate for stabilizing agent; Through allotment, homogeneous, degassed, sterilization, fillingly form a kind of functional calcium fruit enzyme beverage.

Embodiment 8

A kind of raw material of functional calcium fruit ferment consists of: calcium fruit 15 parts, matrimony vine 2 parts, 0.5 part, lutein, taurine 0.6 part, honey 1 part, HFCS 2 parts, granulated sugar 15 parts, xylo-oligosaccharide 0.5 part.

1) fresh calcium fruit through cleaning, go out enzyme or the enzyme that do not go out, broken, adds pectase and cellulase insulation enzymolysis, at the end of enzymolysis, add 1-2 water doubly, leave standstill after stirring, separate section supernatant (subsider juice) is for subsequent use, and its amount is 20% of calcium fruit juice total amount;

3) by step 1) remaining calcium fruit fruit juice and step 2) the wolfberry juice mixing that obtains, adjustment mixed serum concentration, makes the soluble solid content of above-mentioned mixed serum reach 5%, adjust pH 7.0;

4) by step 3) add xylo-oligosaccharide in the slurries that obtain, be cooled to 38 DEG C after sterilizing, inoculation probio carries out logical oxygen, heat-preservation fermentation, and interval supplements HFCS, and maintaining solid content is 5%; Centrifugal filtration after 30 hours of fermenting is removed slag and is obtained calcium fruit ferment stoste; Probio is bacillus coagulans, bacillus licheniformis, and two bacterial strain equal proportions use, and consumption is 2 percent.

5) in calcium fruit ferment stoste, step 1 is added) isolated calcium fruit subsider juice, add lutein, taurine, honey, reach 60% with granulated sugar, syrup adjustment ferment stoste soluble solid content, obtain a kind of functional calcium fruit ferment.

6) get calcium fruit ferment stoste 10 parts, calcium fruit supernatant 5 parts, 1 part, lutein, taurine 1 part, stevioside 1 part, xylo-oligosaccharide 2 parts, honey is appropriate, HFCS is appropriate, and in right amount, pure water is appropriate for stabilizing agent; Through allotment, homogeneous, degassed, sterilization, fillingly form a kind of functional calcium fruit enzyme beverage.

Get the PVPP of 4%, add after swelling with the water soakings of 20 times in isolated calcium fruit supernatant and stir, leave standstill and also can be used for Beverage Service after separating and filtering after 1 hour, PVPP can recycle.

Embodiment 9

A kind of raw material of functional calcium fruit ferment consists of: calcium fruit 10 parts, matrimony vine 1 part, 0.1 part, lutein, taurine 0.4 part, honey 1 part, HFCS 2 parts, granulated sugar 11 parts, xylo-oligosaccharide 0.3 part.

1) fresh calcium fruit through cleaning, go out enzyme or the enzyme that do not go out, broken, keeps fruit stone complete, add pectase and cellulase insulation enzymolysis 3 hours, be separated and remove fruit stone;

3) by step 1) remaining calcium fruit fruit juice and step 2) the wolfberry juice mixing that obtains, adjustment mixed serum concentration, makes the soluble solid content of above-mentioned mixed serum reach 2%, adjust pH 7.5;

4) by step 3) add xylo-oligosaccharide in the slurries that obtain, be cooled to 37 DEG C after sterilizing, inoculation probio carries out logical oxygen, heat-preservation fermentation, and the stage supplements HFCS, and maintaining solid content is 2%; Centrifugation after 30 hours of fermenting obtains clear liquid and concentrate, and concentrate obtains calcium fruit ferment powder through low temperature drying; Probio is bacillus coagulans, and consumption is 2 percent.

5) clear liquid continues inoculation probio and carries out sealed fermenting, stops fermentation when zymotic fluid ph value reaches 3.0, and after room temperature, namely ferment obtains calcium fruit ferment stoste for 30 days; Probio is bifidobacterium bifidum, Lactobacillus casei, Lactobacillus rhamnosus, and each bacterial classification equal proportion uses, and total consumption is 10/1000ths.

6) in calcium fruit ferment stoste, add lutein, taurine, honey, add granulated sugar, syrup makes soluble solid content reach 62%, obtain a kind of functional calcium fruit ferment.

7) get calcium fruit ferment stoste 10 parts, 1 part, lutein, taurine 1 part, stevioside 1 part, xylo-oligosaccharide 1 part, honey is appropriate, HFCS is appropriate, and in right amount, pure water is appropriate for stabilizing agent; Through allotment, homogeneous, degassed, sterilization, fillingly form a kind of functional calcium fruit enzyme beverage.

Embodiment 10

A kind of raw material of functional calcium fruit ferment powder consists of: calcium fruit 15 parts, matrimony vine 2 parts, 0.5 part, lutein, taurine 0.6 part, honey 1 part, HFCS 2 parts, stevioside 0.4 part, compound sugar 0.5 part.

The production method of functional calcium fruit ferment powder comprises following steps:

1) calcium fruit is through cleaning, stoning, broken, making beating;

2) water adding 5 times after matrimony vine cleaning carries out rehydration, making beating;

3) by calcium fruit pulp and wolfberry juice mixing, then use pectase and cellulase 45-50 DEG C of enzymolysis 2 hours, Vacuum Concentration one times, adds honey, HFCS, lutein, taurine, compound sugar, sterilizing;

4) be cooled to 30 DEG C after sterilizing, rear inoculation probio carries out logical oxygen, heat-preservation fermentation, and probio is bifidobacterium longum, Lactobacillus casei, bacillus coagulans, streptococcus thermophilus, Lactobacillus plantarum, and each bacterial classification equal proportion uses, and total consumption is 5 percent;

5) 25 little terminations constantly of fermenting are fermented, and add stevioside, and vacuum drying or vacuum freeze drying become calcium fruit ferment powder;

6) process calcium chankings by above-mentioned calcium fruit ferment powder, calcium chankings comprises effervescent tablet, chewable tablets, lozenge;

7) above-mentioned calcium fruit ferment powder also can be processed into the product form such as particle electuary, capsule.

Embodiment 11

A kind of raw material of functional calcium fruit ferment powder consists of: calcium fruit 15 parts, matrimony vine 2 parts, 0.5 part, lutein, taurine 0.6 part, honey 1 part, stevioside 0.4 part, compound sugar 0.5 part.

1) calcium fruit is through cleaning, stoning, broken, making beating;

3) by calcium pulp and wolfberry fruit syrup mixing, add honey, lutein, taurine, compound sugar, the drying of stevioside final vacuum or vacuum freeze drying and become calcium fruit ferment powder;

4) process calcium chankings by above-mentioned calcium fruit ferment powder, calcium chankings comprises effervescent tablet, chewable tablets, lozenge;

5) above-mentioned calcium fruit ferment powder also can be processed into the product form such as particle electuary, capsule.

Embodiment 12

A kind of raw material of functional calcium fruit ferment powder consists of: calcium fruit 15 parts, matrimony vine 2 parts, 0.5 part, lutein, taurine 0.6 part, 1 part, granulated sugar powder, stevioside 0.4 part, compound sugar 0.5 part.

1) calcium fruit is through cleaning, stoning, broken, making beating;

3) by calcium pulp and wolfberry fruit syrup mixing, vacuum drying or vacuum freeze drying, mix after adding granulated sugar powder, lutein, taurine, compound sugar, stevioside calcium fruit ferment powder;

Above-described embodiment is only used for further illustrating technical scheme of the present invention; but not be limited; the present invention is not limited to embodiment; every above embodiment is done according to technical spirit of the present invention any simple amendment, replacement, equivalent variations and modification, all fall into the protection domain of technical solution of the present invention.

Embodiment 13

Detect the calcium fruit ferment of embodiment 1 gained, testing result is as shown in the table:

The testing result of other embodiment testing results and embodiment 1 is more or less the same.

The calcium content of calcium fruit ferment, up to 43.5mg/100g, is 10 times of apple, and is biological active calcium, be more conducive to absorption of human body.

Within 1985, American scholar people from Bonn pigment existing for the stereoscopic nethike embrane macula area of reference such as (Bone) is the mixture of lutein and luteole, does not detect other carotenoid.Scientific worker other afterwards analyzes the carotenoid in lens, proves also only to detect zeaxanthin in crystalline lens, does not also detect other carotenoid.

A large amount of research reports is by the absorption of lutein and zeaxanthin dietary supplements, and in blood, the concentration of corresponding composition can raise, and the lutein in macula retinae district and zeaxanthin concentration also can increase, and namely macular pigment optical density (MPOD) raises.So the lutein of needed by human body and zeaxanthin can be supplemented by meals.Multinomial research also shows, negative correlation is there is between the nutrition condition of human body lutein and zeaxanthin and age related eye diseases (as AMD) risk, thus specify that lutein and zeaxanthin play a part sight protectio composition, further demonstrate that the intake improving lutein and zeaxanthin effectively can reduce the developing risk of the illness in eye such as AMD, the research being wherein subject to most extensive concern is that the age related eye diseases of 5 years by a definite date carried out in 2006 by American National institute of ophthalmology (NEI) studies 2 (Age-relatedeyediseasestudy2, AREDS2).This research is that its experimenter is more than 4000, and the age is between 50-85 year, and most of experimenter suffers from moderate AMD across the whole America, a random experiment carrying out in more than 80 places.AREDS2 is the largest clinical experimental study of validity by the end of to test lutein, zeaxanthin so far for AMD crowd, object is to analyze supplementary lutein and zeaxanthin for the effect reducing AMD and cataract risk further, and the supplementary intake of its Lutein and zeaxanthin is respectively 10mg/ days and 2mg/ days.In calcium fruit ferment, the content of zeaxanthin and lutein reaches 18.6mg/100g and 33.4mg/100g, can be used as effective meal supplement source of item functional component.

The probiotic test of embodiment 14 calcium of the present invention fruit ferment powder

The calcium fruit ferment powder embodiment of the present invention 1 prepared, being prepared into viable count with sterilized water is 2 × 10 ¹⁰the probiotic solution of CFU/mL, saves backup in 4 DEG C;

1, the 10mL probiotic solution kept of going bail for is injected in test tube 1, adopts ten times of stepwise dilutions to 10 ^-8, get 1mL dilution on flat board, the MRS agar medium being cooled to 45 DEG C poured in dull and stereotyped (sterilizing), shake up rapidly after sterilizing.Again the test tube 2 that 10mL probiotic solution is housed is placed in 70-80 DEG C of water-bath and heats 15-25min, get the probiotic solution after heating and carry out ten times of stepwise dilutions to 10 ^-8, get 1mL dilution on flat board, the MRS agar medium being cooled to 45 DEG C poured in dull and stereotyped (sterilizing) upper and shake up rapidly after sterilizing.Finally the flat board before heating and after heating is all cultivated 24h under 35 DEG C of conditions, calculate the quantity before and after heating.

Result shows, Viable detection reaches 98%.

2, the resistance test of SGF and intestinal juice: the hydrochloric acid 16.4mL adding distil water dilution of getting 100g/L, pH value is made to be respectively 1.5,2.5 and 3.5, get 100mL dilute hydrochloric acid solution, add 1g pepsin respectively, it is made fully to dissolve, obtain SGF, miillpore filter degerming (0.22 μm) is for subsequent use.Get potassium dihydrogen phosphate 6.8g, the 500mL that adds water makes dissolving, by 0.1moL/L sodium hydroxide solution adjust ph to 6.8; Separately get trypsase 10g, the 100mL that adds water makes dissolving, and after two liquid mixing, be diluted with water to 1000ml, obtain simulated intestinal fluid, miillpore filter degerming (0.22 μm) is for subsequent use.Get the probiotic solution that 1mL keeps and join (i.e. ten times of stepwise dilutions) in the SGF of 9mL, and fully mix on the oscillator rapidly, be then placed in 30-45 DEG C of quiescent culture 2-4h.Take out nutrient solution when 1h, 2h, 3h, 4h respectively and count remaining viable count immediately, compare with former viable count, result shows, Viable detection is 98%.Then each 1mL of the nutrient solution digesting different time is taken in simulated gastric fluid, being inoculated in 9mLpH value is respectively in the simulated intestinal fluid of 6.8, be placed in 30-45 DEG C of quiescent culture 2-4h, and respectively 0,3,6,24h sampling, measure its viable count, compare with former viable count, result shows that Viable detection is 99%.

3, simulating the resistance test of cholate: the solution making 1g/L with pancreatin, and the pig cholate adding 0.8% in the solution, is 8.0 with the NaOH adjustment pH of 10%, then uses 0.45 μm of micro-filtrate membrane filtration and degerming.The probiotic solution kept by 0.5mL is inoculated into 4.5mL and simulates in cholate, obtains nutrient solution, count remaining viable count after cultivating 24h.By nutrient solution ten times of stepwise dilutions to 10 in sterile saline ^-8, and pour into MRS, be then placed in 35 DEG C of quiescent culture 24h.Result shows that Viable detection is 99%.

Above result of the test shows, the probio probiotic (heat resistance, resistance to pH, bile tolerance) in calcium fruit ferment powder of the present invention is comparatively strong, and be very applicable to human intestines and stomach's environment, in simulated gastrointestinal environments, survival rate is large, effectively can improve gastrointestinal function.

It should be noted that: calcium prepared by embodiment of the present invention 2-10 fruit ferment powder and solid thereof have above-mentioned experiment effect equally, between each embodiment and little with above-mentioned experiment effect otherness.

Embodiment 15 calcium fruit of the present invention ferment is on the impact of immunity of organisms

1 experiment purpose

By exercise tolerance test (mouse forced swimming), verify raising immunity, the antifatigue effect of calcium of the present invention fruit ferment.

2 experiment materials and reagent

2.1 test sample samples:

Commercially available ferment (G1); Commercially available fruit ferment (G2); Calcium fruit ferment (G3-G12) prepared by embodiment of the present invention 1-9.

2.2 reagent:

Liver/muscle glycogen testing cassete, builds up institute of biological products purchased from Nanjing; The concentrated sulfuric acid (AR), Nanjing Chemistry Reagent Co., Ltd.; Physiological saline, Shangdong Changfu Jiejing Pharmaceutical Industry Co., Ltd..

3. animal used as test

ICR mouse, ♂, cleaning grade, body weight 18-22g, is provided by Ningxia Medical University's comparative medicine center, the free diet of experimental session mouse.

4. key instrument

Aluminum swimming trunk (50cm × 50cm × 40cm), galvanized wire, low-temperature and high-speed centrifuge: 5804R type, Eppendrof company; Water-bath: DK-S26 type, the grand experimental facilities Co., Ltd of upper Nereid; Electronic scale: BS224S type, Sartorius company; Stopwatch, thermometer

5. experiment grouping

5.1 dosage groupings and given the test agent give the time: at random mouse is divided into 13 groups, often organize 10,1st group to the 12nd group respectively to the sample specimens of G1 ~ G12,13rd group is blank group, give isopyknic distilled water, the often every average daily gavage of group 1 time, gavage volume is 0.2ml/10g, gives given the test agent continuously 30 days.

5.2 sample preparations: the 1st group to the 12nd group: take 2.25g sample specimens sample, be assigned to 150ml with distilled water; Blank group (the 13rd group): distilled water 150ml.

6. experimental technique

After 6.1 swimming with a load attached to the body experiment last administration 30min, put mouse in swimming trunk, the depth of water is no less than 30cm, water temperature 25 ± 1 DEG C, the sheet lead of mouse root of the tail portion load 5% body weight, and the swimming of record mouse starts to the dead time, as mouse swimming time.

After 6.2 mice serum urea measure last administration 30min, be not swimming with a load attached to the body 90min in the water of 30 DEG C in temperature, eyeball blood sampling 0.5mL (not adding anti-coagulants) is plucked after rest 60min, put 4 DEG C of refrigerator 3h, the centrifugal 15min of 2000r/min after blood clotting, gets serum and send clinical laboratory of Affiliated Hospital of Ningxia Medical University to detect.

After the mensuration last administration 30min of 6.3 hepatic glycogen, be not swimming with a load attached to the body 90min in the water of 25 ± 1 DEG C in temperature, cervical dislocation puts to death mouse, clean with physiological saline, and with after filter paper suck dry moisture, accurately taking liver 100mg, hepatic glycogen detection kit detects Mouse Liver glycogen content.

Take a blood sample after the mensuration last administration 30min of 6.4 blood lactase acid, then do not bear a heavy burden stops after temperature is the water went swimming 10min of 30 DEG C.Lactic acid instrument assay method: after rest 20min, each blood sampling 20 μ L add in 40 μ L rupture of membranes liquid before swimming, after swimming, after swimming respectively, the smudge cells lactic acid instrument that fully vibrates immediately measures.(blood lactase acid TG-AUC=5 × (the blood lactase acid value of the rear 20min of blood lactase acid value+2 × swimming of the rear 0min of front blood lactase acid value+3 × swimming that swims)

7. observation index walking weight load, blood lactase acid, urea, glycogen initial value

8. statistical method experimental data is used represent, employing t inspection is compared between organizing

9. experimental result

9.1 calcium fruit ferment of the present invention are on the impact of Mouse Weight

Each group of mouse is after giving G1 ~ G12 sample specimens, before, in, shown in post-weight sees the following form respectively, the original body mass of each group of mouse and increase weight body weight and the more equal no difference of science of statistics of control group (P > 0.05), show G1 ~ G12 sample specimens all without obvious toxicity.Experimental result refers to table 5.

The original body mass of table 5 swimming with a load attached to the body experiment mice, mid-term body weight and terminate body weight ( )

9.2 calcium fruit ferment of the present invention are on the impact of mice burden swimming time

After per os gives mouse G1 ~ G12 sample specimens, G1 ~ G2 sample specimens compares with blank group, can obviously extend the mice burden swimming time, there is significant difference (P < 0.05), calcium fruit ferment G3 ~ G12 sample specimens of the present invention compares with blank group, can the significant prolongation mice burden swimming time, there is pole significant difference (P < 0.01), and be obviously better than G1 ~ G2 sample specimens.The results detailed in Table 6.

Table 6 calcium fruit ferment on the impact of mice burden swimming time ( )

" * " p<0.05vs blank;

" * * " p<0.01vs blank;

9.3 calcium fruit ferment of the present invention are on the impact of blood lactase acid before and after mouse movement

After per os gives mouse calcium fruit of the present invention ferment, calcium of the present invention fruit ferment G3 ~ G12 sample specimens to compare with control group blood lactase acid TG-AUC after mouse movement significant difference (P < 0.05), decrease though G1 ~ G2 sample specimens group Mouse Blood lactic acid TG-AUC compares with control group, and no difference of science of statistics (P > 0.05).The results are shown in Table 7.

Table 7 calcium of the present invention fruit ferment on the impact of blood lactase acid level before and after mouse movement ( )

" * " p<0.05vs blank;

9.4 calcium fruit ferment of the present invention are on the impact of Mouse Liver glycogen

After per os gives mouse G1 ~ G12 sample specimens, G1 ~ G2 sample specimens compares with blank group, Mouse Liver glycogen content all has obvious rising, there is significant difference (P < 0.05), calcium fruit ferment G3 ~ G12 sample specimens of the present invention compares with blank group, Mouse Liver glycogen content all has obvious rising, has pole significant difference (P < 0.01), and is obviously better than G1 ~ G2 sample specimens.The results detailed in Table 8.

Table 8 calcium of the present invention fruit ferment on the impact of Mouse Liver glycogen content ( )

" * " p<0.05vs blank;

" * * " p<0.01vs blank;

9.5 calcium fruit ferment of the present invention are on the impact of mice serum urea

After per os gives mouse G1 ~ G12 sample specimens, G1 ~ G2 sample specimens group compares with blank group, after mouse movement, serum urea content all has obvious reduction, there is significant difference (P < 0.05), calcium fruit ferment G3 ~ G12 sample specimens of the present invention compares with blank group, after mouse movement, serum urea content all has obvious reduction, there is pole significant difference (P < 0.01), and be obviously better than G1 ~ G2 sample specimens.The results detailed in Table 9.

Table 9 calcium of the present invention fruit ferment on the impact of mice serum urea content ( )

" * " p<0.05vs blank;

" * * " p<0.01vs blank;

10. experiment conclusion

This experiment is mainly through mice burden swimming experiment, and the deposit simultaneously detecting Mouse Liver glycogen observes the effect that calcium of the present invention fruit ferment improves immunity, antifatigue.Preliminary Results shows below:

1, G3 ~ G12 calcium fruit ferment of the present invention all can extend the mice burden swimming time (P < 0.01), and successful is better than the calcium fruit ferment of other G1 ~ G2.

2, biochemistry detection aspect display, the lactic acid content that after each dosage group of G3 ~ G12 calcium fruit ferment of the present invention all can reduce motion, in mice serum, glucose anerobic glycolysis produces, compare with control group and have significant difference (P < 0.05), and although the calcium of other G1 ~ G2 fruit ferment also can reduce the lactic acid content that in the rear mice serum of motion, glucose anerobic glycolysis produces, but compare with control group, no difference of science of statistics (P > 0.05);

3, each dosage group of G3 ~ G12 calcium fruit ferment of the present invention all can significantly improve the deposit (P < 0.01) of glycogen in mouse liver, and successful is better than the calcium fruit ferment of other G1 ~ G2;

4, high lithemia model finds, G3 ~ G12 calcium fruit ferment of the present invention significantly can reduce the content (P < 0.01) of the rear urea in serum of mouse swimming, and successful is better than other G1 ~ G2 calcium fruit ferment;

11. conclusions

Above-mentioned experiment proves that calcium of the present invention fruit ferment can significantly improve immunity of organisms, improve muscle power and the endurance of mouse, the content of urea in serum and lactic acid after reduction mouse movement, and the deposit of glycogen in mouse liver can be significantly improved, contribute to the fatigue that alleviation exercise load causes; The time that mice burden swimming to power exhausts can be extended.

Embodiment 16 calcium fruit of the present invention ferment mouse intestinal performance test

The calcium fruit ferment prepared by the embodiment of the present invention 2 is tested, and carries out dilution 5 times before experiment to sample.

Choose common Kunming small white mouse 60, male and female half and half, 18-20g, conventional word is supported.Therefrom random choose 40,9:00 gavage Lincomycin Hydrochloride 0.2mL every morning (20mg)/only, other as a control group, every day same time gavage equivalent sterile saline, continuous one week, prepares the mouse model of intestinal bacilli illness.Model group mouse diet declines, and do not occur dead and phenomenon of significantly suffering from diarrhoea, arrange soft excrement, profile normal aqueous divides more, and bedding and padding are moist.By 40 intestinal bacilli illness mouse, be divided into 2 groups at random, one group 20 only as treatment group, the probiotic solution 0.5ml (2 × 10 that every day, gavage was kept ¹⁰cfu/ml)/only, another 20 as natural recovering group, every day same time gavage equivalent sterile saline, continuous two weeks.21 days whole experimental periods, observe growth and the defecation situation of small white mouse every day, weighed in the 8th, 21 day to calcium fruit ferment treatment group and the mouse of natural recovering group, calculate each group of weight average growth rate, result is as table 2; Within every 5 days, survey each group of stool in mice Escherichia coli quantity, calculate average, result is as table 3.Get stool in mice and be about 0.1g, in aseptic operating platform, add 3 beades (adding 0.5mL dilution with 0.1g excrement sample), dilute and inoculate maconkey agar culture medium, calculate every gram of coliform count wet just.

Table 2 mouse Gain weight

Grouping	Average starting weight (g/ only)	Average end heavy (g/ only)	Average growth rate (%)
				Natural recovering group	19.72±1.53	26.41±1.51	33.91 ^a
Treatment group	19.78±1.32	34.58±1.60	74.82 ^b

The situation of coliform count in table 3 stool in mice

Calcium fruit ferment treatment group Mouse Weight average growth rate (74.82%) is significantly higher than natural recovering group (33.91%); Solution rear intestinal Escherichia coli quantity of feeding significantly declines, reduce by 83.89%, be significantly higher than natural recovering group (32.21%), show the probio rapid field planting in small white mouse enteron aisle in calcium of the present invention fruit ferment, form dominant microflora, and effectively suppress the growth and breeding of the pathogens such as Escherichia coli, and resident time is long, continues, effectively improve enteron aisle performance.

It should be noted that: calcium prepared by embodiment of the present invention 1-9 fruit ferment and ferment powder have above-mentioned experiment effect equally, between each embodiment and little with above-mentioned experiment effect otherness.

Claims

1. functional calcium fruit ferment, a calcium fruit enzyme beverage and production method thereof, calcium fruit enzyme beverage with the product in calcium fruit ferment production process for raw material; The raw material of calcium fruit ferment consists of: calcium fruit 1-30 part, matrimony vine 0-5 part, lutein 0-1 part, taurine 0-1 part, compound sugar 0-3 part, the some parts of other auxiliary materials, and the production method of described calcium fruit ferment and beverage thereof comprises following steps:

6) by step 1) isolated calcium fruit supernatant and step 5) the calcium fruit ferment stoste mixing that obtains, then add the auxiliary material such as lutein, taurine, dissolve and mix and obtain functional calcium fruit ferment;

2. a functional calcium fruit ferment, it is characterized in that being made up of solid, liquid two parts, solid portion is the step 4 of claim 1) obtained calcium fruit ferment powder, liquid part is the step 6 of claim 1) calcium that obtains fruit ferment, flexible package punch is equipped with several solid inner wrappings and forms a complete calcium fruit ferment packaging, drinks time edible by after the solid mixing adding an inner wrapping after a certain amount of liquid diluting.

3. the production method of functional calcium fruit ferment according to claim 1 and calcium fruit enzyme beverage thereof, is characterized in that described isolated calcium fruit supernatant carries out gelatin, egg-white powder or PVPP process.

4. a calcium fruit ferment powder, is characterized in that described calcium fruit ferment powder is step 4 in claim 1) in obtained calcium fruit ferment powder; Or formed through concentrated, drying by calcium fruit ferment stoste according to claim 1 or calcium fruit ferment; Also can by step 1 according to claim 1) in calcium fruit after cleaning, de-core, preheating, making beating after the concentrated calcium fruit juice inoculation probiotics fermention of concentrated gained, concentrate after adding lutein, taurine, compound sugar, drying form; Also can by step 1 according to claim 1) in calcium fruit after cleaning, de-core, preheating, pull an oar the calcium fruit juice that obtains through concentrated, after adding lutein, taurine, compound sugar, drying forms; Also can by step 1 according to claim 1) in calcium fruit pull an oar after cleaning, de-core, preheating gained the sterilization of calcium fruit juice after inoculate probiotics fermention, concentrated after adding lutein, taurine, compound sugar after fermentation ends, drying forms; Also can by step 1 according to claim 1) in calcium fruit after cleaning, de-core, preheating through dry, pulverize after add lutein, taurine, compound sugar mix and form.

5. a calcium chankings, is characterized in that described calcium chankings is processed by calcium fruit powder according to claim 4; Described calcium chankings comprises effervescent tablet, chewable tablets, lozenge; This calcium fruit powder also can be processed into the product form such as particle electuary, capsule.

6. according to claim 1, functional calcium fruit ferment described in 4, calcium fruit enzyme beverage and production method and calcium fruit ferment powder, it is characterized in that described probio is bifidobacterium adolescentis, animal bifidobacteria (bifidobacterium lactis), bifidobacterium bifidum, bifidobacterium breve, bifidobacterium infantis, bifidobacterium longum, bacillus coagulans, Lactobacillus casei, Lactobacillus crispatus, lactobacillus delbruockii subspecies bulgaricus (lactobacillus bulgaricus), bacillus cereus, Lactobacillus delbrueckii breast subspecies, lactobacillus fermenti, lactobacillus gasseri, Lactobacillus helveticus, Yue Shi lactobacillus, lactobacillus paraceasi, lactobacillus reuteri, Lactobacillus rhamnosus, bacillus subtilis, Lactobacillus salivarius, streptococcus thermophilus, Lactobacillus plantarum, bacillus licheniformis, lactobacillus paraceasi, Lactobacillus rhamnosus, lactobacillus acidophilus, leuconostoc mesenteroides subsp mesenteroides, kluyveromyces marxianus, Pediococcus acidilactici, one or more in Pediococcus pentosaceus.