Background technology
Root-knot nematode and Cyst nematode, be the two class nematodes the heaviest to the harm of agricultural production, the crop of main harm has cucumber, peanut, paddy rice, soybean, wheat, tobacco, potato, oranges and tangerines, corn etc.Nematode generally causes crop failure 10% ~ 40%, can cause the underproduction of 80%, even have no harvest time serious.
By being tackle the two large approach that root-knot nematode and Cyst nematode endanger with the cultural control method and chemical pesticide control method of non-host crop crop rotation or rice field-upland field rotation.Along with the phonamiphos of chemical pesticide, carbofuran, the contour toxicant of terbufos restriction or prohibit the use, Avermectin becomes the anti-medicament of master of current nematode, but due to long-term single use, nematode produces larger resistance to it.Therefore, a kind of high-efficiency low-toxicity nematoda control medicament is badly in need of to break away from the single situation of current medicament.
Fosthiazate, chemical name: O-ethyl-S-sec-butyl-2-oxo-1,3-thiazoles alkane-3-base Thiophosphonate, has and tag and systemic action, can be killed by plant roots absorption and invade the root-knot nematode that crop root forms root nodule.Fosthiazate toxicity is lower, meets export of farm produce standard, is mainly used in preventing and treating nematode, aphid etc., has special efficacy to root-knot nematode, pratylenchus, Cyst nematode.
Trichoderma harzianum is the microbial bactericide produced by Bai Wo limited company of the U.S., is mainly used in the fungal disease preventing and treating plant root.Trichoderma harzianum effectively can not only suppress the growth of germ, and can produce the compound of stimulating plant growth and inducing plant defense reaction, improves the microenvironment of root system, strengthens growing way and the resistance against diseases of plant, improves output and the income of crop.Trichoderma harzianum is applied to control root-knot nematode relevant report, " qualification of trichoderma harzianum strain TRI2 and the preventive and therapeutic effect to cucumber root-knot nematode thereof " delivered as Ma Jinhui, Zhu Pingping is pointed out, Trichoderma TRI2 zymotic fluid 100% can kill root-knot nematode when 48h, in pot experiment, make cucumber root knot decline rate reach 63.5%, in field, 72.2% is reached to the control efficiency of cucumber root-knot nematode, and to Avermectin, there is similar control efficiency in field.
At present, using fosthiazate, trichoderma harzianum as the also not relevant report of the granular formulation for killing nematode of active ingredient.
Summary of the invention
The object of the invention is to, a kind of granular formulation for killing nematode containing fosthiazate is provided, there is high-efficiency low-toxicity, the preventive effect of root-knot nematode, Cyst nematode can be significantly improved, reduce the advantage of agricultural chemicals mu input cost.
The technical solution used in the present invention is: a kind of granular formulation for killing nematode containing fosthiazate, active component is made up of fosthiazate and trichoderma harzianum.
Described active component, trichoderma harzianum is decided to be 100% female medicine with female medicine of 30,000,000,000 CFU/ grams, the weight ratio of fosthiazate and trichoderma harzianum is 5-90:1, fosthiazate content is in the composition 2.5% ~ 15%, and trichoderma harzianum content is in the composition 0.3 hundred million CFU/ grams ~ 1.5 hundred million CFU/ gram.
The adjuvant that the described granular formulation for killing nematode containing fosthiazate uses comprise filler, binding agent, dispersant, wetting agent, disintegrant, sustained release agent, stabilizing agent or other be of value to the active component material that plays of stable and drug effect in the formulation, be all conventional in agriculture field preparation or allow the various compositions that use.
The present invention has the following advantages and significant technique effect:
(1) medicament high-efficiency low-toxicity, compares carbofuran, and the toxicity of medicament to people and animals obviously reduces, and avoids the risk of production and application person poultry poisoning.
(2) active component fosthiazate and trichoderma harzianum Synergistic, use with in the reasonable scope, can reduce the amount of application of each single dose, is conducive to reducing drug cost and reducing environmental pollution.
(3) trichoderma harzianum can improve the microenvironment of soil, makes plant root growth more healthy, has good control efficiency to the root rot in soil-borne disease, southern blight, damping off, damping off.
Embodiment
Be described further technical scheme of the present invention and technique effect below by way of specific embodiment, percentage of the present invention is all weight percentage.
One, indoor joint toxicity measuring test
The indoor joint toxicity measuring test of fosthiazate, trichoderma harzianum and mixed agent thereof, 5 concentration are diluted by active constituent content gradient series with fosthiazate, trichoderma harzianum and mixed agent thereof, compare with sterile water, test with Meloidogyne incognita second instar larvae as examination worm, blank is set, fosthiazate, trichoderma harzianum single dose and mixture preparation thereof are adopted dip method, carries out toxicity test and do statistical analysis.
Evaluate the synergistic effect of pharmacy mix with the co-toxicity coefficient method (CTC) of the abundant method of Sun Yun, namely CTC≤80 are antagonism, and 80<CTC<120 is summation action, and CTC >=120 are synergistic effect.Statistics is as shown in table 1:
Table 1 fosthiazate, trichoderma harzianum and mixed agent thereof are to the Toxicity Determination table of cucumber root-knot nematode
The measurement result of indoor joint toxicity measuring test shows, when the mixed ratio of fosthiazate and trichoderma harzianum is 2:1, co-toxicity coefficient (CTC) is 110.6, and both mixtures are summation action; When the mixed ratio of fosthiazate and trichoderma harzianum is 1:5, co-toxicity coefficient (CTC) is less than 80, and both mixtures are antagonism; When the mixed ratio of fosthiazate and trichoderma harzianum is 5-90:1 scope, co-toxicity coefficient (CTC) is all greater than 120, and namely synergistic effect is obvious.
Two, product embodiments
Embodiment 1:2.5% fosthiazate 0.3 hundred million cfu/ gram of trichoderma harzianum granule
Formula: the weight ratio according to fosthiazate and trichoderma harzianum is 25:1, take the former medicine 2.63kg of 95% fosthiazate, 30000000000 cfu/ grams of Trichoderma harzianum starter medicine 0.1kg, fatty alcohol-polyoxyethylene ether 2.0kg, lauryl sodium sulfate 2.5kg, polyvinyl alcohol 1.5kg, carboxymethyl cellulose 2.5kg, kaolin complements to 100kg.
Embodiment 2:5.0% fosthiazate 0.5 hundred million cfu/ gram of trichoderma harzianum granule
Formula: the weight ratio according to fosthiazate and trichoderma harzianum is 30:1, take the former medicine 5.26kg of 95% fosthiazate, 30000000000 cfu/ grams of Trichoderma harzianum starter medicine 0.17kg, naphthalenesulfonate formaldehyde condensation compound 2.5kg, lauryl sodium sulfate 1.5kg, polyvinyl alcohol 2.5kg, attapulgite 5kg, bentonite complements to 100kg.
Embodiment 3:10.0% fosthiazate 0.5 hundred million cfu/ gram of trichoderma harzianum granule
Formula: the weight ratio according to fosthiazate and trichoderma harzianum is 60:1, take the former medicine 5.26kg of 95% fosthiazate, 30000000000 cfu/ grams of Trichoderma harzianum starter medicine 0.17kg, carboxymethyl cellulose 2.5kg, lauryl sodium sulfate 2.5kg, polyvinyl alcohol 1.5kg, attapulgite 15kg, kaolin complements to 100kg.
Embodiment 4:10.0% fosthiazate 1.5 hundred million cfu/ gram of trichoderma harzianum granule
Formula: the weight ratio according to fosthiazate and trichoderma harzianum is 20:1, takes the former medicine 10.53kg of 95% fosthiazate, 30,000,000,000 cfu/ grams of Trichoderma harzianum starter medicine 0.5kg, naphthalenesulfonate formaldehyde condensation compound 3.5kg, lauryl sodium sulfate 2.5kg, polyethylene glycol 2.0kg, bentonite supplies 100kg.
Embodiment 5:15.0% fosthiazate 1.0 hundred million cfu/ gram of trichoderma harzianum granule
Formula: the weight ratio according to fosthiazate and trichoderma harzianum is 45:1, takes the former medicine 15.79kg of 95% fosthiazate, 30,000,000,000 cfu/ grams of Trichoderma harzianum starter medicine 0.33kg, lauryl sodium sulfate 2.5kg, carboxymethyl cellulose 1.5kg, diatomite 15kg, bentonite supplies 100kg.
Preparation method: mixed by the recipe ingredient described in above-described embodiment 1-5, pulverizes, stirs, then use screw extruder pelletizer granulation after adding water-wet, and sieving after drying and obtaining containing active component is the granule of fosthiazate and trichoderma harzianum.
Three, products application embodiment
Embodiment 1 to embodiment 5 products obtained therefrom is carried out the control of cucumber root-knot nematode, test process arranges as follows:
Process 1:2.5% fosthiazate 0.3 hundred million cfu/ gram of trichoderma harzianum granule, amount of application is 30kg/ mu.
Process 2:5.0% fosthiazate 0.5 hundred million cfu/ gram of trichoderma harzianum granule, amount of application is 15kg/ mu.。
Process 3:10.0% fosthiazate 0.5 hundred million cfu/ gram of trichoderma harzianum granule, amount of application is 8kg/ mu.
Process 4:10.0% fosthiazate 1.5 hundred million cfu/ gram of trichoderma harzianum granule, amount of application is 8kg/ mu.
Process 5:15.0% fosthiazate 1.0 hundred million cfu/ gram of trichoderma harzianum granule, amount of application is 5kg/ mu.
Process 6:10.0% fosthiazate granule, amount of application is 15kg/ mu.
Process 7:5.0 hundred million cfu/ gram of trichoderma harzianum granule, amount of application is 45kg/ mu.
Process 8: blank.
Above-mentioned process is used cucumber vegetative growth phase, and within after medicine 30 days, 50 days, carry out root-knot nematode respectively and to cause harm investigation, carry out determination of yield during results, final statistics is as shown in the table: